Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0011557

Physical Location & Seq

Genomic Coordinates Forward (+)

51440665 .. 51443676

Gene Structure 3012 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G025380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGGCCGTTCGTCAGACCAACGCAACGGTGACTATACTTCACAACGCCGTCATCGTCACTGTGGACCCCGACGGCCGGGTCTTCCGCGACGGCGCCGTCGTCATCGAGCATGACTCTATCAGAGCCGTTGGTCAATCGTCGGCTATTCTTCACCAGTTCTCTGCTCAAGCCCATCAGCTTCTCAATCTGAACGGCCAAATCATACTTCCTGGATTCATCAACACACACGTACACACATCTCAACAACTAGCGAGGGGGATAGCCGATGACGTCGATTTGATGACGTGGCTGAAGATTCATACATTTCTACTCTGCTTTGTGGAATTGAGCTCATTCACTCTGGTAACTTGCTTTGCTGAAGCAGGAGGGCAACATGTGTCAGGAATGGCGAGGGCGGTTGAGTTACTGGGTATACGGGCATGTTTAGCCGAGTCCATCATGGACTCTGGCGACGGCCTGCCCACAACTTGGGCTGCTCGGAGTACTGATGATTGTATTCAGTCTCAGAAAGAGCTTTACAAGAAGCACCATAATACAGGTGATGGGCGCATCAGAGTGTGGTTAGGAATTAGGCAGATCATGAATGCAACTGATGGTTTACTACTTGCAACAAGGGACATTGCCAAAAAACTCGAAACTGGAATCCACATGCATGTTGCAGAGATAGCATATGAGAATCAGTTTGTGACAGATACACGAAAAGTTAACCATGGAACAGTCACACACTTGGAGCAGATAGATTTTCTCCATAACAATTTGCTAGCAGCTCATACTGTTTGGGTCAACGATTCTGAGATTGATTGTCTTTCAAGGGCTGAGGTAAAAGTATCTCACTGTCCTGCTGCTGCAATGCGAATGCTTGGATTTGCTCCTATTAGAGAGATGATTGGTGCTGGAATCTGTGTCTCCTTGGGAACAGATGGGGCCCCATCAAATAATAGAATGAGCTTAGTTGATGAGATGTACCTGGCCTCTCTGATTAACAAAGGACGGGAAGTTTACAAGAATGGAACCACTGATCCTACTGCTCTTCCTTCTGAAACAGTTCTCAAGATGGCGACTATAAATGGTGCAAAATCTGTACTCTGGGATAATGAAATAGGGTCCCTTGAAGTTGGGAAAAAGGCTGACCTGGTCGTTATCAATCCTTCCACTTGGACAATGATTCCTCTCCATGACTGCATTTCGAGCCTTGTGTATTCTATGCGAACTGAAAATATCGTCTCTGTAATGTGCAATGGCAAGTGGATTATGCAGGATAAGAAGATTCTGAATGTGGATGAGGAACACGTTATTTCAATGGCAAAGCAAGCTTCAGCGGAAATTGTAAAGAGAGCAGGCATCAAAATACCAGACAGGATGAACTTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidohydro_1

Protein Analysis

457

Amino Acids

50.16

Weight (kDa)

6.16

Isoelectric Point (pI)

24.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_1	PF01979	67 - 417	3.8e-49	Amidohydrolase family
Amidohydro_3	PF07969	208 - 384	1.8e-12	Amidohydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011557)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g23250 FvH4_4g23250 FvH4_4g23250 FvH4_4g23250
malus_domestica	MD10G1063400.v1.1 MD13G1123400.v1.1 MD16G1124200.v1.1
prunus_persica	Prupe.1G221400_v2.0.a1 Prupe.4G226800_v2.0.a1
pyrus_communis	pycom16g10610
rosa_chinensis	RchiOBHm_Chr4g0429611
rosa_laevigata	RLG00000007058
rosa_multiflora	Rmu_sc0006590.1_g000013 Rmu_sc0013109.1_g000001 Rmu_sc0017032.1_g000003
rosa_roxburghii	Rroxscaffold_5G00371300
rosa_rugosa	Rorug04G0237300
rosa_samantha	Rh4BG298900 Rh4CG314500
rosa_wichuraiana	Rw4G025380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	77, 95
AatII	GACGTC	1 cut(s)	273
AccB1I	GGYRCC	1 cut(s)	92
AccB7I	CCANNNNNTGG	1 cut(s)	468
AccI	GTMKAC	1 cut(s)	412
AccII	CGCG	1 cut(s)	87
AciI	CCGC	3 cut(s)	85, 395, 1319
AclWI	GGATC	1 cut(s)	1013
AcoI	YGGCCR	3 cut(s)	3, 73, 193
AcuI	CTGAAG	3 cut(s)	311, 378, 1299
AcyI	GRCGYC	2 cut(s)	93, 270
AfaI	GTAC	4 cut(s)	231, 484, 965, 1083
AfiI	CCNNNNNNNGG	2 cut(s)	75, 468
AflIII	ACRYGT	3 cut(s)	226, 373, 1288
AgsI	TTSAA	3 cut(s)	810, 1112, 1299
AjiI	CACGTC	1 cut(s)	285
AjnI	CCWGG	3 cut(s)	208, 966, 1131
AjuI	GAANNNNNNNTTGG	2 cut(s)	189, 221
AloI	GAACNNNNNNTCC	2 cut(s)	1003, 1035
AluBI	AGCT	6 cut(s)	178, 330, 514, 767, 948, 1313
AluI	AGCT	6 cut(s)	178, 330, 514, 767, 948, 1313
Alw21I	GWGCWC	1 cut(s)	332
Alw26I	GTCTC	3 cut(s)	507, 910, 1228
AlwI	GGATC	1 cut(s)	1013
AoxI	GGCC	6 cut(s)	3, 73, 193, 454, 924, 969
ApaI	GGGCCC	1 cut(s)	928
ApeKI	GCWGC	4 cut(s)	473, 764, 842, 845
Asp700I	GAANNNNTTC	1 cut(s)	1044
AspLEI	GCGC	2 cut(s)	95, 549
AspS9I	GGNCC	4 cut(s)	64, 924, 925, 1104
AsuC2I	CCSGG	1 cut(s)	77
AsuHPI	GGTGA	3 cut(s)	40, 143, 551
AsuNHI	GCTAGC	1 cut(s)	760
AvaII	GGWCC	2 cut(s)	64, 1104
BaeGI	GKGCMC	1 cut(s)	928
BanI	GGYRCC	1 cut(s)	92
BanII	GRGCYC	2 cut(s)	332, 928
BbsI	GAAGAC	1 cut(s)	73
Bbv12I	GWGCWC	1 cut(s)	332
BbvCI	CCTCAGC	1 cut(s)	816
BbvI	GCAGC	4 cut(s)	460, 776, 829, 832
BccI	CCATC	7 cut(s)	180, 443, 536, 587, 914, 937, 1048
BceAI	ACGGC	7 cut(s)	32, 80, 88, 106, 110, 208, 469
BciT130I	CCWGG	3 cut(s)	210, 968, 1133
BcnI	CCSGG	1 cut(s)	77
BcoDI	GTCTC	3 cut(s)	507, 910, 1228
BfaI	CTAG	2 cut(s)	248, 761
BfoI	RGCGCY	1 cut(s)	96
BisI	GCNGC	4 cut(s)	474, 765, 843, 846
BlsI	GCNGC	4 cut(s)	475, 766, 844, 847
BmcAI	AGTACT	1 cut(s)	484
Bme1390I	CCNGG	4 cut(s)	77, 210, 968, 1133
Bme18I	GGWCC	2 cut(s)	64, 1104
BmgBI	CACGTC	1 cut(s)	285
BmgT120I	GGNCC	4 cut(s)	64, 924, 925, 1104
BmiI	GGNNCC	8 cut(s)	66, 94, 925, 926, 927, 1012, 1105, 1106
BmrFI	CCNGG	4 cut(s)	77, 210, 968, 1133
BmrI	ACTGGG	1 cut(s)	416
BmsI	GCATC	2 cut(s)	558, 1350
BmtI	GCTAGC	1 cut(s)	764
BmuI	ACTGGG	1 cut(s)	416
BpiI	GAAGAC	1 cut(s)	73
BplI	GAGNNNNNCTC	2 cut(s)	98, 130
Bpu10I	CCTNAGC	1 cut(s)	816
BpuEI	CTTGAG	2 cut(s)	150, 1034
BpuMI	CCSGG	1 cut(s)	77
BsaAI	YACGTR	1 cut(s)	229
BsaHI	GRCGYC	2 cut(s)	93, 270
BsaJI	CCNNGG	2 cut(s)	709, 909
Bsc4I	CCNNNNNNNGG	2 cut(s)	75, 468
Bse1I	ACTGG	3 cut(s)	154, 411, 643
Bse3DI	GCAATG	3 cut(s)	618, 855, 1243
BseBI	CCWGG	3 cut(s)	210, 968, 1133
BseDI	CCNNGG	2 cut(s)	709, 909
BseGI	GGATG	2 cut(s)	1285, 1365
BseLI	CCNNNNNNNGG	2 cut(s)	75, 468
BseMI	GCAATG	3 cut(s)	618, 855, 1243
BseMII	CTCAG	3 cut(s)	518, 783, 807
BseNI	ACTGG	3 cut(s)	154, 411, 643
BseSI	GKGCMC	1 cut(s)	928
BseX3I	CGGCCG	1 cut(s)	73
BseXI	GCAGC	4 cut(s)	460, 776, 829, 832
Bsh1236I	CGCG	1 cut(s)	87
Bsh1285I	CGRYCG	1 cut(s)	76
BshFI	GGCC	6 cut(s)	5, 75, 195, 456, 926, 971
BshNI	GGYRCC	1 cut(s)	92
BsiEI	CGRYCG	1 cut(s)	76
BsiHKAI	GWGCWC	1 cut(s)	332
BsiSI	CCGG	1 cut(s)	76
BslFI	GGGAC	2 cut(s)	629, 1090
BslI	CCNNNNNNNGG	2 cut(s)	75, 468
BsmAI	GTCTC	3 cut(s)	507, 910, 1228
BsmBI	CGTCTC	1 cut(s)	1228
BsmFI	GGGAC	2 cut(s)	629, 1090
BsmI	GAATGC	2 cut(s)	589, 861
BsnI	GGCC	6 cut(s)	5, 75, 195, 456, 926, 971
Bsp120I	GGGCCC	1 cut(s)	924
Bsp1286I	GDGCHC	2 cut(s)	332, 928
Bsp143I	GATC	2 cut(s)	576, 1018
Bsp19I	CCATGG	1 cut(s)	709
BspACI	CCGC	3 cut(s)	85, 395, 1319
BspANI	GGCC	6 cut(s)	5, 75, 195, 456, 926, 971
BspCNI	CTCAG	3 cut(s)	517, 784, 808
BspFNI	CGCG	1 cut(s)	87
BspHI	TCATGA	1 cut(s)	579
BspLI	GGNNCC	8 cut(s)	66, 94, 925, 926, 927, 1012, 1105, 1106
BspOI	GCTAGC	1 cut(s)	764
BspPI	GGATC	1 cut(s)	1013
BspQI	GCTCTTC	1 cut(s)	1035
BspT107I	GGYRCC	1 cut(s)	92
BsrDI	GCAATG	3 cut(s)	618, 855, 1243
BsrI	ACTGG	3 cut(s)	154, 411, 643
BssECI	CCNNGG	2 cut(s)	709, 909
BssMI	GATC	2 cut(s)	576, 1018
BssNAI	GTATAC	1 cut(s)	413
BssNI	GRCGYC	2 cut(s)	93, 270
BssT1I	CCWWGG	2 cut(s)	709, 909
Bst1107I	GTATAC	1 cut(s)	413
Bst2UI	CCWGG	3 cut(s)	210, 968, 1133
Bst4CI	ACNGT	6 cut(s)	28, 61, 718, 775, 836, 1045
Bst6I	CTCTTC	1 cut(s)	1035
BstACI	GRCGYC	2 cut(s)	93, 270
BstBAI	YACGTR	1 cut(s)	229
BstC8I	GCNNGC	4 cut(s)	458, 762, 1311, 1339
BstDEI	CTNAG	4 cut(s)	504, 792, 816, 949
BstDSI	CCRYGG	1 cut(s)	709
BstF5I	GGATG	2 cut(s)	1285, 1365
BstFNI	CGCG	1 cut(s)	87
BstH2I	RGCGCY	1 cut(s)	96
BstHHI	GCGC	2 cut(s)	95, 549
BstKTI	GATC	2 cut(s)	579, 1021
BstMAI	GTCTC	3 cut(s)	507, 910, 1228
BstMBI	GATC	2 cut(s)	576, 1018
BstMCI	CGRYCG	1 cut(s)	76
BstMWI	GCNNNNNNNGC	4 cut(s)	425, 665, 1188, 1310
BstNI	CCWGG	3 cut(s)	210, 968, 1133
BstNSI	RCATGY	4 cut(s)	377, 423, 652, 656
BstSCI	CCNGG	4 cut(s)	75, 208, 966, 1131
BstSLI	GKGCMC	1 cut(s)	928
BstUI	CGCG	1 cut(s)	87
BstV1I	GCAGC	4 cut(s)	460, 776, 829, 832
BstV2I	GAAGAC	1 cut(s)	73
BstZ17I	GTATAC	1 cut(s)	413
BstZI	CGGCCG	1 cut(s)	73
BsuRI	GGCC	6 cut(s)	5, 75, 195, 456, 926, 971
BtgI	CCRYGG	1 cut(s)	709
BtrI	CACGTC	1 cut(s)	285
BtsCI	GGATG	2 cut(s)	1285, 1365
BtsIMutI	CAGTG	3 cut(s)	57, 832, 1014
Cac8I	GCNNGC	4 cut(s)	458, 762, 1311, 1339
CciI	TCATGA	1 cut(s)	579
CfoI	GCGC	2 cut(s)	95, 549
Cfr13I	GGNCC	4 cut(s)	64, 924, 925, 1104
CsiI	ACCWGGT	1 cut(s)	1131
Csp6I	GTAC	4 cut(s)	230, 483, 964, 1082
CviAII	CATG	9 cut(s)	110, 374, 420, 439, 580, 649, 653, 710, 1175
CviQI	GTAC	4 cut(s)	230, 483, 964, 1082
DdeI	CTNAG	4 cut(s)	504, 792, 816, 949
DinI	GGCGCC	1 cut(s)	94
DpnI	GATC	2 cut(s)	578, 1020
DpnII	GATC	2 cut(s)	576, 1018
DrdI	GACNNNNNNGTC	2 cut(s)	77, 95
DseDI	GACNNNNNNGTC	2 cut(s)	77, 95
EaeI	YGGCCR	3 cut(s)	3, 73, 193
EagI	CGGCCG	1 cut(s)	73
Eam1104I	CTCTTC	1 cut(s)	1035
EarI	CTCTTC	1 cut(s)	1035
Ecl136II	GAGCTC	1 cut(s)	330
EclXI	CGGCCG	1 cut(s)	73
Eco130I	CCWWGG	2 cut(s)	709, 909
Eco24I	GRGCYC	2 cut(s)	332, 928
Eco47I	GGWCC	2 cut(s)	64, 1104
Eco52I	CGGCCG	1 cut(s)	73
Eco53kI	GAGCTC	1 cut(s)	330
Eco57I	CTGAAG	3 cut(s)	311, 378, 1299
EcoICRI	GAGCTC	1 cut(s)	330
EcoO109I	RGGNCCY	3 cut(s)	924, 925, 1104
EcoRII	CCWGG	3 cut(s)	208, 966, 1131
EcoT14I	CCWWGG	2 cut(s)	709, 909
EcoT22I	ATGCAT	1 cut(s)	654
EcoT38I	GRGCYC	2 cut(s)	332, 928
EgeI	GGCGCC	1 cut(s)	94
EheI	GGCGCC	1 cut(s)	94
ErhI	CCWWGG	2 cut(s)	709, 909
Esp3I	CGTCTC	1 cut(s)	1228
FaeI	CATG	9 cut(s)	113, 377, 423, 442, 583, 652, 656, 713, 1178
FaqI	GGGAC	2 cut(s)	629, 1090
FatI	CATG	9 cut(s)	109, 373, 419, 438, 579, 648, 652, 709, 1174
FauNDI	CATATG	1 cut(s)	670
FblI	GTMKAC	1 cut(s)	412
Fnu4HI	GCNGC	4 cut(s)	474, 765, 843, 846
FokI	GGATG	1 cut(s)	1292
FriOI	GRGCYC	2 cut(s)	332, 928
Fsp4HI	GCNGC	4 cut(s)	474, 765, 843, 846
FspBI	CTAG	2 cut(s)	248, 761
GlaI	GCGC	2 cut(s)	94, 548
GluI	GCNGC	4 cut(s)	474, 765, 843, 846
HaeII	RGCGCY	1 cut(s)	96
HaeIII	GGCC	6 cut(s)	5, 75, 195, 456, 926, 971
HapII	CCGG	1 cut(s)	76
HhaI	GCGC	2 cut(s)	95, 549
Hin1I	GRCGYC	2 cut(s)	93, 270
Hin1II	CATG	9 cut(s)	113, 377, 423, 442, 583, 652, 656, 713, 1178
Hin6I	GCGC	2 cut(s)	93, 547
HinP1I	GCGC	2 cut(s)	93, 547
HincII	GTYRAC	2 cut(s)	706, 784
HindII	GTYRAC	2 cut(s)	706, 784
HindIII	AAGCTT	1 cut(s)	1311
HpaI	GTTAAC	1 cut(s)	706
HpaII	CCGG	1 cut(s)	76
HphI	GGTGA	3 cut(s)	40, 143, 551
Hpy166II	GTNNAC	7 cut(s)	64, 232, 413, 599, 706, 784, 1000
Hpy188III	TCNNGA	3 cut(s)	381, 580, 1051
Hpy8I	GTNNAC	7 cut(s)	64, 232, 413, 599, 706, 784, 1000
Hpy99I	CGWCG	6 cut(s)	74, 92, 101, 142, 275, 455
HpyAV	CCTTC	2 cut(s)	1044, 1158
HpyCH4III	ACNGT	6 cut(s)	28, 61, 718, 775, 836, 1045
HpyCH4IV	ACGT	4 cut(s)	228, 270, 284, 1290
HpyCH4V	TGCA	9 cut(s)	587, 608, 652, 659, 848, 1073, 1182, 1236, 1255
HpyF10VI	GCNNNNNNNGC	4 cut(s)	425, 665, 1188, 1310
HpyF3I	CTNAG	4 cut(s)	504, 792, 816, 949
HpySE526I	ACGT	4 cut(s)	228, 270, 284, 1290
Hsp92I	GRCGYC	2 cut(s)	93, 270
Hsp92II	CATG	9 cut(s)	113, 377, 423, 442, 583, 652, 656, 713, 1178
HspAI	GCGC	2 cut(s)	93, 547
KasI	GGCGCC	1 cut(s)	92
KflI	GGGWCCC	1 cut(s)	1104
KspAI	GTTAAC	1 cut(s)	706
Kzo9I	GATC	2 cut(s)	576, 1018
LguI	GCTCTTC	1 cut(s)	1035
LmnI	GCTCC	2 cut(s)	730, 874
Lsp1109I	GCAGC	4 cut(s)	460, 776, 829, 832
LweI	GCATC	2 cut(s)	558, 1350
MabI	ACCWGGT	1 cut(s)	1131
MaeI	CTAG	2 cut(s)	248, 761
MaeII	ACGT	4 cut(s)	228, 270, 284, 1290
MaeIII	GTNAC	6 cut(s)	28, 55, 343, 402, 685, 718
MalI	GATC	2 cut(s)	578, 1020
MboI	GATC	2 cut(s)	576, 1018
MboII	GAAGA	5 cut(s)	73, 140, 304, 1022, 1276
MhlI	GDGCHC	2 cut(s)	332, 928
MluCI	AATT	4 cut(s)	323, 567, 754, 1323
Mly113I	GGCGCC	1 cut(s)	93
MlyI	GAGTC	3 cut(s)	107, 437, 440
MnlI	CCTC	7 cut(s)	246, 359, 384, 811, 982, 1179, 1276
Mph1103I	ATGCAT	1 cut(s)	654
MroXI	GAANNNNTTC	1 cut(s)	1044
MseI	TTAA	2 cut(s)	705, 981
MslI	CAYNNNNRTG	2 cut(s)	554, 651
MspA1I	CMGCKG	1 cut(s)	1319
MspI	CCGG	1 cut(s)	76
MspR9I	CCNGG	4 cut(s)	77, 210, 968, 1133
Mva1269I	GAATGC	2 cut(s)	589, 861
MvaI	CCWGG	3 cut(s)	210, 968, 1133
MvnI	CGCG	1 cut(s)	87
MwoI	GCNNNNNNNGC	4 cut(s)	425, 665, 1188, 1310
NarI	GGCGCC	1 cut(s)	93
NciI	CCSGG	1 cut(s)	77
NcoI	CCATGG	1 cut(s)	709
NdeI	CATATG	1 cut(s)	670
NdeII	GATC	2 cut(s)	576, 1018
NheI	GCTAGC	1 cut(s)	760
NlaIII	CATG	9 cut(s)	113, 377, 423, 442, 583, 652, 656, 713, 1178
NlaIV	GGNNCC	8 cut(s)	66, 94, 925, 926, 927, 1012, 1105, 1106
NmeAIII	GCCGAG	1 cut(s)	454
NmuCI	GTSAC	4 cut(s)	28, 55, 685, 718
NsiI	ATGCAT	1 cut(s)	654
NspI	RCATGY	4 cut(s)	377, 423, 652, 656
PagI	TCATGA	1 cut(s)	579
PciI	ACATGT	1 cut(s)	373
PciSI	GCTCTTC	1 cut(s)	1035
PcsI	WCGNNNNNNNCGW	2 cut(s)	51, 96
PctI	GAATGC	2 cut(s)	589, 861
PdmI	GAANNNNTTC	1 cut(s)	1044
PfeI	GAWTC	8 cut(s)	213, 295, 642, 676, 788, 897, 1165, 1267
PflFI	GACNNNGTC	1 cut(s)	1133
PflMI	CCANNNNNTGG	1 cut(s)	468
PfoI	TCCNGGA	1 cut(s)	208
PkrI	GCNGC	4 cut(s)	475, 766, 844, 847
PleI	GAGTC	3 cut(s)	107, 437, 439
PluTI	GGCGCC	1 cut(s)	96
PpsI	GAGTC	3 cut(s)	107, 437, 439
Ppu21I	YACGTR	1 cut(s)	229
PpuMI	RGGWCCY	1 cut(s)	1104
PscI	ACATGT	1 cut(s)	373
Psp124BI	GAGCTC	1 cut(s)	332
Psp5II	RGGWCCY	1 cut(s)	1104
Psp6I	CCWGG	3 cut(s)	208, 966, 1131
PspGI	CCWGG	3 cut(s)	208, 966, 1131
PspN4I	GGNNCC	8 cut(s)	66, 94, 925, 926, 927, 1012, 1105, 1106
PspOMI	GGGCCC	1 cut(s)	924
PspPI	GGNCC	4 cut(s)	64, 924, 925, 1104
PspPPI	RGGWCCY	1 cut(s)	1104
PsyI	GACNNNGTC	1 cut(s)	1133
RsaI	GTAC	4 cut(s)	231, 484, 965, 1083
RsaNI	GTAC	4 cut(s)	230, 483, 964, 1082
RseI	CAYNNNNRTG	2 cut(s)	554, 651
SacI	GAGCTC	1 cut(s)	332
SapI	GCTCTTC	1 cut(s)	1035
SaqAI	TTAA	2 cut(s)	705, 981
SatI	GCNGC	4 cut(s)	474, 765, 843, 846
Sau3AI	GATC	2 cut(s)	576, 1018
Sau96I	GGNCC	4 cut(s)	64, 924, 925, 1104
ScaI	AGTACT	1 cut(s)	484
SchI	GAGTC	3 cut(s)	107, 437, 440
ScrFI	CCNGG	4 cut(s)	77, 210, 968, 1133
SduI	GDGCHC	2 cut(s)	332, 928
SexAI	ACCWGGT	1 cut(s)	1131
SfaNI	GCATC	2 cut(s)	558, 1350
SfoI	GGCGCC	1 cut(s)	94
SinI	GGWCC	2 cut(s)	64, 1104
SmiMI	CAYNNNNRTG	2 cut(s)	554, 651
SmlI	CTYRAG	2 cut(s)	165, 1049
SmoI	CTYRAG	2 cut(s)	165, 1049
Sse9I	AATT	4 cut(s)	323, 567, 754, 1323
SsiI	CCGC	3 cut(s)	85, 395, 1319
SspDI	GGCGCC	1 cut(s)	92
SspMI	CTAG	2 cut(s)	248, 761
SstI	GAGCTC	1 cut(s)	332
StyD4I	CCNGG	4 cut(s)	75, 208, 966, 1131
StyI	CCWWGG	2 cut(s)	709, 909
TaaI	ACNGT	6 cut(s)	28, 61, 718, 775, 836, 1045
TaiI	ACGT	4 cut(s)	231, 273, 287, 1293
TaqI	TCGA	4 cut(s)	105, 273, 633, 1187
TasI	AATT	4 cut(s)	323, 567, 754, 1323
TatI	WGTACW	2 cut(s)	482, 1081
TfiI	GAWTC	8 cut(s)	213, 295, 642, 676, 788, 897, 1165, 1267
Tru1I	TTAA	2 cut(s)	705, 981
Tru9I	TTAA	2 cut(s)	705, 981
TscAI	CASTG	3 cut(s)	64, 839, 1021
TseFI	GTSAC	4 cut(s)	28, 55, 685, 718
TseI	GCWGC	4 cut(s)	473, 764, 842, 845
Tsp45I	GTSAC	4 cut(s)	28, 55, 685, 718
TspDTI	ATGAA	4 cut(s)	205, 287, 596, 1110
TspRI	CASTG	3 cut(s)	64, 839, 1021
Tth111I	GACNNNGTC	1 cut(s)	1133
Van91I	CCANNNNNTGG	1 cut(s)	468
VpaK11BI	GGWCC	2 cut(s)	64, 1104
XceI	RCATGY	4 cut(s)	377, 423, 652, 656
XmiI	GTMKAC	1 cut(s)	412
XmnI	GAANNNNTTC	1 cut(s)	1044
XspI	CTAG	2 cut(s)	248, 761
ZraI	GACGTC	1 cut(s)	271
ZrmI	AGTACT	1 cut(s)	484
Zsp2I	ATGCAT	1 cut(s)	654

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.