Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011557

Physical Location & Seq

Genomic Coordinates Reverse (-)

8974817 .. 8978742

Gene Structure 3926 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1124200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGCGTCTCCGACCAACTTCGCCTCAGCAGTGACTATACTTCACAACGCTACCATCGTCACCATGGATTCCGACAGCCGCGTCTTCCGCAACGGCGGGATCGTCATCGAGCGAGACGCGATCAGAGCCGTCGGTCAGTCTTCCGATATACTCCGCCAGTTCTCCGCCCTCGCCCACCGGGTTGTCGATCTCAACGGCCAAATCGTCCTCCCTGGACTGATCAATACGCACGTGCACACCTCGCAGCAACTGGGAAGAGGGATTGCAGATGACGTGGATTTGATGACGTGGCTGCACGATCGGATTTGGCCCTACGAGTCCAACATGAGCGAGGAAGATTCGTACATTTCCACCTTGCTTTGTGGCATAGAGCTCATTCACTCTGGTGTAACTTGCTTTGCTGAAGCAGGAGGGCAACATGTGTCGGGAATGGCGAGGGCGGTGGAGTTGCTGGGGTTAAGGGCGTGTTTGGCTGAGTCCACCATGGACTCAGGTGACGGCCTGCCCAAGTCTTGGGCAGGTCGGAGCGCCGGTGATTGCATCCAGTCTCAGAAAGAGCTTTACAAGAAGCACCATAACACCGGAGACGGGCGCATCAGAGTGTGGCTAGGAATTAGGCAGATTATGAATGCAACTGATGATTTGCTGCTTGCAACAAGGGACACTGCCAAGGAACTGAAAACTGGAATCCACATGCATGTTGCAGAGATAGCATACGAGAATCAATTTGTGACAGAGACGCGAAAAGTTGACCATGGAACGATAACACACTTGGAGAATATACAGTTCCTCCAGAACAATTTGCTAGCTGCCCACACAGTTTGGGTTAATCCTTCTCAGATTGATTGTCTTTCAAGGGGCGGGGTCAAAGTATCTCATTGCCCTGCCGCTGCGATGCGAATGCTTGGATTTGCACCTATTAGAGAGATGCTGGATGCTGGCATCTGTGTCTCTTTGGGAACAGATGGGGCACCCTCGAATAACAGAATGAGCATAGTTGATGAGATGTACCTGGCTTCTCTGATTAACAAAGGACGGGAAGTTCATACAAATGGAACTACTGATCCTACTGCTCTTCCTGCTGAAACAGTTCTTGAGTTGGTGACTATAAATGGTGCAAAATCAGTACTTTGGGATAATGAAATAGGATCCCTTGAGGTCGGCAAAAAGGCTGACTTGGTTGTTATCAATCCTTCCTCTTGGACCATGGTTCCTCTCCATGACAGCATTTCCAGCCTTGTGTACAGTATGCGAACCGAAAATGTAGTCTCTGTAATGTGCAATGGTCTGTGGATTATGAAGGATAAGAAGATTTTGAACGTGGATGAGGAAGAAGTTCTTGCAAAGGCAAAGCAAGCTTCCGCAGAAATTCTGAAGCGGGCAGGGATCAAAATACCAAGTAGAATGAATTTTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidohydro_1

Protein Analysis

473

Amino Acids

51.57

Weight (kDa)

5.86

Isoelectric Point (pI)

32.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_1	PF01979	68 - 430	2.7e-57	Amidohydrolase family
Amidohydro_3	PF07969	222 - 403	1.6e-13	Amidohydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011557)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g23250 FvH4_4g23250 FvH4_4g23250 FvH4_4g23250
malus_domestica	MD10G1063400.v1.1 MD13G1123400.v1.1 MD16G1124200.v1.1
prunus_persica	Prupe.1G221400_v2.0.a1 Prupe.4G226800_v2.0.a1
pyrus_communis	pycom16g10610
rosa_chinensis	RchiOBHm_Chr4g0429611
rosa_laevigata	RLG00000007058
rosa_multiflora	Rmu_sc0006590.1_g000013 Rmu_sc0013109.1_g000001 Rmu_sc0017032.1_g000003
rosa_roxburghii	Rroxscaffold_5G00371300
rosa_rugosa	Rorug04G0237300
rosa_samantha	Rh4BG298900 Rh4CG314500
rosa_wichuraiana	Rw4G025380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	80
Acc36I	ACCTGC	1 cut(s)	509
AccB1I	GGYRCC	1 cut(s)	970
AccB7I	CCANNNNNTGG	1 cut(s)	513
AccII	CGCG	3 cut(s)	81, 119, 742
AclWI	GGATC	5 cut(s)	107, 1058, 1143, 1156, 1394
AcoI	YGGCCR	1 cut(s)	196
AcsI	RAATTY	2 cut(s)	1368, 1408
AcuI	CTGAAG	2 cut(s)	423, 1394
AcvI	CACGTG	1 cut(s)	232
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	344, 1010, 1128, 1244
AfiI	CCNNNNNNNGG	2 cut(s)	513, 588
AflIII	ACRYGT	1 cut(s)	418
AgsI	TTSAA	2 cut(s)	855, 1318
AjiI	CACGTC	2 cut(s)	274, 288
AjnI	CCWGG	2 cut(s)	211, 1011
AleI	CACNNNNGTG	1 cut(s)	384
AloI	GAACNNNNNNTCC	4 cut(s)	1026, 1048, 1058, 1080
AluBI	AGCT	4 cut(s)	373, 559, 809, 1358
AluI	AGCT	4 cut(s)	373, 559, 809, 1358
Alw21I	GWGCWC	2 cut(s)	237, 375
Alw26I	GTCTC	7 cut(s)	12, 108, 552, 579, 731, 955, 1273
Alw44I	GTGCAC	1 cut(s)	233
AlwI	GGATC	5 cut(s)	107, 1058, 1143, 1156, 1394
AlwNI	CAGNNNCTG	1 cut(s)	250
AoxI	GGCC	3 cut(s)	196, 308, 499
ApaLI	GTGCAC	1 cut(s)	233
ApeKI	GCWGC	5 cut(s)	244, 292, 646, 809, 890
ApoI	RAATTY	2 cut(s)	1368, 1408
ArsI	GACNNNNNNTTYG	2 cut(s)	1251, 1283
Asp700I	GAANNNNTTC	2 cut(s)	1089, 1335
AspLEI	GCGC	2 cut(s)	530, 594
AspS9I	GGNCC	2 cut(s)	309, 1203
AsuC2I	CCSGG	1 cut(s)	179
AsuHPI	GGTGA	4 cut(s)	52, 506, 545, 1114
AsuNHI	GCTAGC	1 cut(s)	805
AvaII	GGWCC	1 cut(s)	1203
BaeGI	GKGCMC	2 cut(s)	237, 973
BamHI	GGATCC	1 cut(s)	1148
BanI	GGYRCC	1 cut(s)	970
BanII	GRGCYC	1 cut(s)	375
BbrPI	CACGTG	1 cut(s)	232
BbsI	GAAGAC	2 cut(s)	76, 132
Bbv12I	GWGCWC	2 cut(s)	237, 375
BbvCI	CCTCAGC	1 cut(s)	25
BbvI	GCAGC	5 cut(s)	256, 279, 633, 796, 877
BccI	CCATC	2 cut(s)	62, 959
BceAI	ACGGC	4 cut(s)	109, 113, 211, 514
BcgI	CGANNNNNNTGC	2 cut(s)	883, 917
BciT130I	CCWGG	2 cut(s)	213, 1013
BclI	TGATCA	1 cut(s)	219
BcnI	CCSGG	1 cut(s)	179
BcoDI	GTCTC	7 cut(s)	12, 108, 552, 579, 731, 955, 1273
BfaI	CTAG	2 cut(s)	608, 806
BfoI	RGCGCY	1 cut(s)	531
BfuAI	ACCTGC	1 cut(s)	509
BisI	GCNGC	7 cut(s)	79, 245, 293, 647, 810, 888, 891
BlsI	GCNGC	7 cut(s)	80, 246, 294, 648, 811, 889, 892
BmcAI	AGTACT	1 cut(s)	1128
Bme1390I	CCNGG	3 cut(s)	179, 213, 1013
Bme18I	GGWCC	1 cut(s)	1203
BmgBI	CACGTC	2 cut(s)	274, 288
BmgT120I	GGNCC	2 cut(s)	309, 1203
BmiI	GGNNCC	3 cut(s)	972, 1150, 1212
BmrFI	CCNGG	3 cut(s)	179, 213, 1013
BmrI	ACTGGG	1 cut(s)	260
BmsI	GCATC	6 cut(s)	549, 603, 885, 918, 925, 951
BmtI	GCTAGC	1 cut(s)	809
BmuI	ACTGGG	1 cut(s)	260
BpiI	GAAGAC	2 cut(s)	76, 132
BpmI	CTGGAG	1 cut(s)	776
Bpu10I	CCTNAGC	1 cut(s)	25
BpuEI	CTTGAG	2 cut(s)	1115, 1175
BpuMI	CCSGG	1 cut(s)	179
BsaAI	YACGTR	1 cut(s)	232
BsaBI	GATNNNNATC	1 cut(s)	104
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	6 cut(s)	63, 211, 483, 669, 754, 1206
BsaWI	WCCGGW	1 cut(s)	581
BsaXI	ACNNNNNCTCC	4 cut(s)	517, 547, 774, 804
Bsc4I	CCNNNNNNNGG	2 cut(s)	513, 588
Bse118I	RCCGGY	1 cut(s)	530
Bse1I	ACTGG	4 cut(s)	157, 255, 544, 688
Bse3DI	GCAATG	2 cut(s)	877, 1288
Bse8I	GATNNNNATC	1 cut(s)	104
BseBI	CCWGG	2 cut(s)	213, 1013
BseDI	CCNNGG	6 cut(s)	63, 211, 483, 669, 754, 1206
BseGI	GGATG	3 cut(s)	540, 940, 1330
BseJI	GATNNNNATC	1 cut(s)	104
BseLI	CCNNNNNNNGG	2 cut(s)	513, 588
BseMI	GCAATG	2 cut(s)	877, 1288
BseMII	CTCAG	5 cut(s)	39, 465, 504, 563, 851
BseNI	ACTGG	4 cut(s)	157, 255, 544, 688
BseSI	GKGCMC	2 cut(s)	237, 973
BseXI	GCAGC	5 cut(s)	256, 279, 633, 796, 877
BseYI	CCCAGC	1 cut(s)	451
BsgI	GTGCAG	1 cut(s)	278
Bsh1236I	CGCG	3 cut(s)	81, 119, 742
Bsh1285I	CGRYCG	1 cut(s)	301
BshFI	GGCC	3 cut(s)	198, 310, 501
BshNI	GGYRCC	1 cut(s)	970
BsiEI	CGRYCG	1 cut(s)	301
BsiHKAI	GWGCWC	2 cut(s)	237, 375
BsiSI	CCGG	3 cut(s)	178, 531, 582
BslFI	GGGAC	1 cut(s)	674
BslI	CCNNNNNNNGG	2 cut(s)	513, 588
BsmAI	GTCTC	7 cut(s)	12, 108, 552, 579, 731, 955, 1273
BsmBI	CGTCTC	4 cut(s)	12, 108, 579, 731
BsmFI	GGGAC	1 cut(s)	674
BsmI	GAATGC	2 cut(s)	634, 906
BsnI	GGCC	3 cut(s)	198, 310, 501
Bsp1286I	GDGCHC	3 cut(s)	237, 375, 973
Bsp1407I	TGTACA	1 cut(s)	1242
Bsp143I	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
Bsp19I	CCATGG	4 cut(s)	63, 483, 754, 1206
BspANI	GGCC	3 cut(s)	198, 310, 501
BspCNI	CTCAG	5 cut(s)	38, 466, 503, 562, 850
BspFNI	CGCG	3 cut(s)	81, 119, 742
BspLI	GGNNCC	3 cut(s)	972, 1150, 1212
BspMI	ACCTGC	1 cut(s)	509
BspOI	GCTAGC	1 cut(s)	809
BspPI	GGATC	5 cut(s)	107, 1058, 1143, 1156, 1394
BspQI	GCTCTTC	1 cut(s)	1080
BspT107I	GGYRCC	1 cut(s)	970
BsrDI	GCAATG	2 cut(s)	877, 1288
BsrFI	RCCGGY	1 cut(s)	530
BsrGI	TGTACA	1 cut(s)	1242
BsrI	ACTGG	4 cut(s)	157, 255, 544, 688
BssAI	RCCGGY	1 cut(s)	530
BssECI	CCNNGG	6 cut(s)	63, 211, 483, 669, 754, 1206
BssMI	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	5 cut(s)	63, 483, 669, 754, 1206
Bst2UI	CCWGG	2 cut(s)	213, 1013
Bst4CI	ACNGT	4 cut(s)	786, 820, 1090, 1247
Bst6I	CTCTTC	2 cut(s)	250, 1080
BstACI	GRCGYC	1 cut(s)	5
BstAUI	TGTACA	1 cut(s)	1242
BstBAI	YACGTR	1 cut(s)	232
BstC8I	GCNNGC	6 cut(s)	503, 651, 807, 940, 1356, 1380
BstDEI	CTNAG	5 cut(s)	25, 474, 490, 549, 837
BstDSI	CCRYGG	4 cut(s)	63, 483, 754, 1206
BstF5I	GGATG	3 cut(s)	540, 940, 1330
BstFNI	CGCG	3 cut(s)	81, 119, 742
BstH2I	RGCGCY	1 cut(s)	531
BstHHI	GCGC	2 cut(s)	530, 594
BstKTI	GATC	8 cut(s)	102, 123, 190, 222, 301, 1066, 1151, 1389
BstMAI	GTCTC	7 cut(s)	12, 108, 552, 579, 731, 955, 1273
BstMBI	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
BstMCI	CGRYCG	1 cut(s)	301
BstMWI	GCNNNNNNNGC	8 cut(s)	87, 125, 241, 470, 710, 1079, 1233, 1355
BstNI	CCWGG	2 cut(s)	213, 1013
BstNSI	RCATGY	3 cut(s)	422, 697, 701
BstSCI	CCNGG	3 cut(s)	177, 211, 1011
BstSLI	GKGCMC	2 cut(s)	237, 973
BstUI	CGCG	3 cut(s)	81, 119, 742
BstV1I	GCAGC	5 cut(s)	256, 279, 633, 796, 877
BstV2I	GAAGAC	2 cut(s)	76, 132
BstX2I	RGATCY	1 cut(s)	1148
BstYI	RGATCY	1 cut(s)	1148
BsuRI	GGCC	3 cut(s)	198, 310, 501
BtgI	CCRYGG	4 cut(s)	63, 483, 754, 1206
BtgZI	GCGATG	1 cut(s)	908
BtrI	CACGTC	2 cut(s)	274, 288
BtsCI	GGATG	3 cut(s)	540, 940, 1330
BtsI	GCAGTG	2 cut(s)	36, 663
BtsIMutI	CAGTG	2 cut(s)	36, 663
BveI	ACCTGC	1 cut(s)	509
Cac8I	GCNNGC	6 cut(s)	503, 651, 807, 940, 1356, 1380
CaiI	CAGNNNCTG	1 cut(s)	250
CfoI	GCGC	2 cut(s)	530, 594
Cfr10I	RCCGGY	1 cut(s)	530
Cfr13I	GGNCC	2 cut(s)	309, 1203
CseI	GACGC	3 cut(s)	70, 125, 748
Csp6I	GTAC	4 cut(s)	343, 1009, 1127, 1243
CspCI	CAANNNNNGTGG	2 cut(s)	803, 838
CviAII	CATG	9 cut(s)	64, 325, 419, 484, 694, 698, 755, 1207, 1220
CviQI	GTAC	4 cut(s)	343, 1009, 1127, 1243
DdeI	CTNAG	5 cut(s)	25, 474, 490, 549, 837
DpnI	GATC	8 cut(s)	101, 122, 189, 221, 300, 1065, 1150, 1388
DpnII	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
DrdI	GACNNNNNNGTC	1 cut(s)	80
DseDI	GACNNNNNNGTC	1 cut(s)	80
EaeI	YGGCCR	1 cut(s)	196
Eam1104I	CTCTTC	2 cut(s)	250, 1080
EarI	CTCTTC	2 cut(s)	250, 1080
EciI	GGCGGA	2 cut(s)	143, 154
Ecl136II	GAGCTC	1 cut(s)	373
Eco130I	CCWWGG	5 cut(s)	63, 483, 669, 754, 1206
Eco24I	GRGCYC	1 cut(s)	375
Eco47I	GGWCC	1 cut(s)	1203
Eco53kI	GAGCTC	1 cut(s)	373
Eco57I	CTGAAG	2 cut(s)	423, 1394
Eco72I	CACGTG	1 cut(s)	232
EcoICRI	GAGCTC	1 cut(s)	373
EcoRII	CCWGG	2 cut(s)	211, 1011
EcoT14I	CCWWGG	5 cut(s)	63, 483, 669, 754, 1206
EcoT22I	ATGCAT	1 cut(s)	699
EcoT38I	GRGCYC	1 cut(s)	375
ErhI	CCWWGG	5 cut(s)	63, 483, 669, 754, 1206
Esp3I	CGTCTC	4 cut(s)	12, 108, 579, 731
FaeI	CATG	9 cut(s)	67, 328, 422, 487, 697, 701, 758, 1210, 1223
FalI	AAGNNNNNCTT	4 cut(s)	1160, 1192, 1323, 1355
FaqI	GGGAC	1 cut(s)	674
FatI	CATG	9 cut(s)	63, 324, 418, 483, 693, 697, 754, 1206, 1219
FauI	CCCGC	3 cut(s)	89, 854, 1371
FbaI	TGATCA	1 cut(s)	219
Fnu4HI	GCNGC	7 cut(s)	79, 245, 293, 647, 810, 888, 891
FokI	GGATG	3 cut(s)	527, 947, 1337
FriOI	GRGCYC	1 cut(s)	375
Fsp4HI	GCNGC	7 cut(s)	79, 245, 293, 647, 810, 888, 891
FspBI	CTAG	2 cut(s)	608, 806
GlaI	GCGC	2 cut(s)	529, 593
GluI	GCNGC	7 cut(s)	79, 245, 293, 647, 810, 888, 891
GsaI	CCCAGC	1 cut(s)	455
GsuI	CTGGAG	1 cut(s)	776
HaeII	RGCGCY	1 cut(s)	531
HaeIII	GGCC	3 cut(s)	198, 310, 501
HapII	CCGG	3 cut(s)	178, 531, 582
HgaI	GACGC	3 cut(s)	70, 125, 748
HhaI	GCGC	2 cut(s)	530, 594
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	9 cut(s)	67, 328, 422, 487, 697, 701, 758, 1210, 1223
Hin6I	GCGC	2 cut(s)	528, 592
HinP1I	GCGC	2 cut(s)	528, 592
HincII	GTYRAC	1 cut(s)	751
HindII	GTYRAC	1 cut(s)	751
HindIII	AAGCTT	1 cut(s)	1356
HinfI	GANTC	7 cut(s)	68, 317, 338, 476, 488, 687, 721
HpaII	CCGG	3 cut(s)	178, 531, 582
HphI	GGTGA	4 cut(s)	52, 506, 545, 1114
Hpy166II	GTNNAC	4 cut(s)	235, 480, 751, 1243
Hpy188III	TCNNGA	3 cut(s)	426, 793, 1094
Hpy8I	GTNNAC	4 cut(s)	235, 480, 751, 1243
Hpy99I	CGWCG	1 cut(s)	134
HpyAV	CCTTC	3 cut(s)	843, 1203, 1294
HpyCH4III	ACNGT	4 cut(s)	786, 820, 1090, 1247
HpyCH4IV	ACGT	4 cut(s)	231, 273, 287, 1320
HpyF10VI	GCNNNNNNNGC	8 cut(s)	87, 125, 241, 470, 710, 1079, 1233, 1355
HpyF3I	CTNAG	5 cut(s)	25, 474, 490, 549, 837
HpySE526I	ACGT	4 cut(s)	231, 273, 287, 1320
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	9 cut(s)	67, 328, 422, 487, 697, 701, 758, 1210, 1223
HspAI	GCGC	2 cut(s)	528, 592
Ksp22I	TGATCA	1 cut(s)	219
Kzo9I	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
LguI	GCTCTTC	1 cut(s)	1080
LmnI	GCTCC	1 cut(s)	525
Lsp1109I	GCAGC	5 cut(s)	256, 279, 633, 796, 877
LweI	GCATC	6 cut(s)	549, 603, 885, 918, 925, 951
MaeI	CTAG	2 cut(s)	608, 806
MaeII	ACGT	4 cut(s)	231, 273, 287, 1320
MaeIII	GTNAC	6 cut(s)	31, 58, 388, 494, 730, 1102
MalI	GATC	8 cut(s)	101, 122, 189, 221, 300, 1065, 1150, 1388
MboI	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
MboII	GAAGA	7 cut(s)	76, 132, 267, 347, 1067, 1321, 1343
MflI	RGATCY	1 cut(s)	1148
MhlI	GDGCHC	3 cut(s)	237, 375, 973
MluCI	AATT	5 cut(s)	612, 725, 799, 1368, 1408
MlyI	GAGTC	3 cut(s)	326, 482, 485
MmeI	TCCRAC	4 cut(s)	35, 96, 345, 503
Mph1103I	ATGCAT	1 cut(s)	699
MroXI	GAANNNNTTC	2 cut(s)	1089, 1335
MseI	TTAA	3 cut(s)	458, 828, 1026
MslI	CAYNNNNRTG	4 cut(s)	384, 599, 696, 1050
MspA1I	CMGCKG	1 cut(s)	890
MspI	CCGG	3 cut(s)	178, 531, 582
MspR9I	CCNGG	3 cut(s)	179, 213, 1013
Mva1269I	GAATGC	2 cut(s)	634, 906
MvaI	CCWGG	2 cut(s)	213, 1013
MvnI	CGCG	3 cut(s)	81, 119, 742
MwoI	GCNNNNNNNGC	8 cut(s)	87, 125, 241, 470, 710, 1079, 1233, 1355
NciI	CCSGG	1 cut(s)	179
NcoI	CCATGG	4 cut(s)	63, 483, 754, 1206
NdeII	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
NheI	GCTAGC	1 cut(s)	805
NlaIII	CATG	9 cut(s)	67, 328, 422, 487, 697, 701, 758, 1210, 1223
NlaIV	GGNNCC	3 cut(s)	972, 1150, 1212
NmuCI	GTSAC	5 cut(s)	31, 58, 494, 730, 1102
NsiI	ATGCAT	1 cut(s)	699
NspI	RCATGY	3 cut(s)	422, 697, 701
OliI	CACNNNNGTG	1 cut(s)	384
PciI	ACATGT	1 cut(s)	418
PciSI	GCTCTTC	1 cut(s)	1080
PcsI	WCGNNNNNNNCGW	4 cut(s)	54, 99, 201, 431
PctI	GAATGC	2 cut(s)	634, 906
PdmI	GAANNNNTTC	2 cut(s)	1089, 1335
PfeI	GAWTC	4 cut(s)	68, 338, 687, 721
PflMI	CCANNNNNTGG	1 cut(s)	513
PkrI	GCNGC	7 cut(s)	80, 246, 294, 648, 811, 889, 892
Ple19I	CGATCG	1 cut(s)	301
PleI	GAGTC	3 cut(s)	325, 482, 484
PmaCI	CACGTG	1 cut(s)	232
PmlI	CACGTG	1 cut(s)	232
PpsI	GAGTC	3 cut(s)	325, 482, 484
Ppu21I	YACGTR	1 cut(s)	232
PscI	ACATGT	1 cut(s)	418
Psp124BI	GAGCTC	1 cut(s)	375
Psp6I	CCWGG	2 cut(s)	211, 1011
PspCI	CACGTG	1 cut(s)	232
PspFI	CCCAGC	1 cut(s)	451
PspGI	CCWGG	2 cut(s)	211, 1011
PspN4I	GGNNCC	3 cut(s)	972, 1150, 1212
PspPI	GGNCC	2 cut(s)	309, 1203
PstNI	CAGNNNCTG	1 cut(s)	250
PsuI	RGATCY	1 cut(s)	1148
PvuI	CGATCG	1 cut(s)	301
RsaI	GTAC	4 cut(s)	344, 1010, 1128, 1244
RsaNI	GTAC	4 cut(s)	343, 1009, 1127, 1243
RseI	CAYNNNNRTG	4 cut(s)	384, 599, 696, 1050
SacI	GAGCTC	1 cut(s)	375
SapI	GCTCTTC	1 cut(s)	1080
SaqAI	TTAA	3 cut(s)	458, 828, 1026
SatI	GCNGC	7 cut(s)	79, 245, 293, 647, 810, 888, 891
Sau3AI	GATC	8 cut(s)	99, 120, 187, 219, 298, 1063, 1148, 1386
Sau96I	GGNCC	2 cut(s)	309, 1203
ScaI	AGTACT	1 cut(s)	1128
SchI	GAGTC	3 cut(s)	326, 482, 485
ScrFI	CCNGG	3 cut(s)	179, 213, 1013
SduI	GDGCHC	3 cut(s)	237, 375, 973
SfaNI	GCATC	6 cut(s)	549, 603, 885, 918, 925, 951
SgrAI	CRCCGGYG	1 cut(s)	530
SinI	GGWCC	1 cut(s)	1203
SmiMI	CAYNNNNRTG	4 cut(s)	384, 599, 696, 1050
SmlI	CTYRAG	2 cut(s)	1094, 1154
SmoI	CTYRAG	2 cut(s)	1094, 1154
Sse9I	AATT	5 cut(s)	612, 725, 799, 1368, 1408
SspMI	CTAG	2 cut(s)	608, 806
SstI	GAGCTC	1 cut(s)	375
StyD4I	CCNGG	3 cut(s)	177, 211, 1011
StyI	CCWWGG	5 cut(s)	63, 483, 669, 754, 1206
TaaI	ACNGT	4 cut(s)	786, 820, 1090, 1247
TaiI	ACGT	4 cut(s)	234, 276, 290, 1323
TaqI	TCGA	3 cut(s)	108, 186, 977
TaqII	GACCGA	1 cut(s)	122
TasI	AATT	5 cut(s)	612, 725, 799, 1368, 1408
TatI	WGTACW	2 cut(s)	1126, 1242
TauI	GCSGC	2 cut(s)	81, 890
TfiI	GAWTC	4 cut(s)	68, 338, 687, 721
Tru1I	TTAA	3 cut(s)	458, 828, 1026
Tru9I	TTAA	3 cut(s)	458, 828, 1026
TscAI	CASTG	2 cut(s)	36, 670
TseFI	GTSAC	5 cut(s)	31, 58, 494, 730, 1102
TseI	GCWGC	5 cut(s)	244, 292, 646, 809, 890
Tsp45I	GTSAC	5 cut(s)	31, 58, 494, 730, 1102
TspDTI	ATGAA	4 cut(s)	641, 1034, 1155, 1313
TspRI	CASTG	2 cut(s)	36, 670
Van91I	CCANNNNNTGG	1 cut(s)	513
VneI	GTGCAC	1 cut(s)	233
VpaK11BI	GGWCC	1 cut(s)	1203
XapI	RAATTY	2 cut(s)	1368, 1408
XceI	RCATGY	3 cut(s)	422, 697, 701
XcmI	CCANNNNNNNNNTGG	1 cut(s)	61
XmnI	GAANNNNTTC	2 cut(s)	1089, 1335
XspI	CTAG	2 cut(s)	608, 806
ZrmI	AGTACT	1 cut(s)	1128
Zsp2I	ATGCAT	1 cut(s)	699

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.