MD10G1065500.v1.1

RNA recognition motif 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011943
Physical Location & Seq
Reverse (-)
8946772 .. 8948299
1528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1065500.v1.1.491

Sequence Viewer

Length: 516 bp
ATGATTTTGTTATTGACGAGAAACATACTAGCGCTTTGTTTCTTGGTTTTGAGATTTTGGTTGATAATTGCGTTCTTGAGTTCATCTAATTGGTGCACCCGGGATATGCTGATGGCGTTTCTGGATGCCCATTGTGCTGAGGAGAATGATAAACCGGATGTAGGAGATGAGATTTCGTCCTATGATTTCTTATATCTGCCTATAGACTTCAATACTGGGTTTAACAAAGGGTATGCGTTCGTCAACTTCACCAACAGCAAGGCAGTCTGGAAATTTTCTGAAGGGACTGGTGGGAAGACTTGGGATCTTTTCTACTCTTCAAAGAAACGTGAAATTGCCTACGCCAAAATCCAGGGCAAGAAGGAGCTGGTAAGTCATTTCGAGACGATGAGGTTTCCTTGTGCATCAGAGGACGTGCTGCCGGTGTGTTTCGACCCACCTCGAGACGGTTCCAGGATGTCTGTTCAGATCTCGACTGTAGGCCATGCAGTGGATCTTAATCGACATGACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_2

Protein Analysis

172

Amino Acids

19.54

Weight (kDa)

5.78

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_2 PF04059 59 - 127 2e-20 RNA recognition motif 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07930 AT5G07930 AT5G07930
fragaria_vesca FvH4_2g10100 FvH4_2g10100
malus_domestica MD10G1065500.v1.1
prunus_persica Prupe.8G087500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0267061
rosa_laevigata RLG00000014045
rosa_multiflora Rmu_co8325855.1_g000001 Rmu_co8394061.1_g000001 Rmu_sc0013804.1_g000004
rosa_roxburghii Rroxscaffold_7G00201180
rosa_rugosa Rorug06G0028800
rosa_samantha Rh6AG150300 Rh6BG151200 Rh6CG146600 Rh6DG135300
rosa_wichuraiana Rw6G013030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 490
AclWI GGATC 2 cut(s) 312, 501
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 1 cut(s) 300
AfeI AGCGCT 1 cut(s) 33
AfiI CCNNNNNNNGG 3 cut(s) 161, 446, 490
AgsI TTSAA 2 cut(s) 211, 321
AjiI CACGTC 1 cut(s) 415
AjnI CCWGG 2 cut(s) 351, 452
AluBI AGCT 1 cut(s) 367
AluI AGCT 1 cut(s) 367
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 2 cut(s) 377, 438
Alw44I GTGCAC 1 cut(s) 94
AlwI GGATC 2 cut(s) 312, 501
Ama87I CYCGRG 2 cut(s) 99, 441
Aor51HI AGCGCT 1 cut(s) 33
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 94
ApeKI GCWGC 1 cut(s) 418
ApoI RAATTY 1 cut(s) 272
AspLEI GCGC 1 cut(s) 34
AsuC2I CCSGG 2 cut(s) 100, 101
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 2 cut(s) 99, 441
BaeGI GKGCMC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 98
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 405
BccI CCATC 1 cut(s) 106
BciT130I CCWGG 2 cut(s) 353, 454
BcnI CCSGG 2 cut(s) 100, 101
BcoDI GTCTC 2 cut(s) 377, 438
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 2 cut(s) 201, 477
BfoI RGCGCY 1 cut(s) 35
BglII AGATCT 1 cut(s) 468
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
Bme1390I CCNGG 4 cut(s) 100, 101, 353, 454
BmeT110I CYCGRG 2 cut(s) 99, 441
BmgBI CACGTC 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 451
BmrFI CCNGG 4 cut(s) 100, 101, 353, 454
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 2 cut(s) 115, 413
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 302
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 97
BpuMI CCSGG 2 cut(s) 100, 101
BsaBI GATNNNNATC 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 99, 352
BsaWI WCCGGW 1 cut(s) 154
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 446, 490
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 220, 292
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 2 cut(s) 353, 454
BseDI CCNNGG 2 cut(s) 99, 352
BseGI GGATG 3 cut(s) 130, 163, 462
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 3 cut(s) 161, 446, 490
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 2 cut(s) 220, 292
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 98
BseXI GCAGC 1 cut(s) 405
BshFI GGCC 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 2 cut(s) 99, 441
BsiSI CCGG 3 cut(s) 100, 155, 422
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 161, 446, 490
BsmAI GTCTC 2 cut(s) 377, 438
BsmBI CGTCTC 2 cut(s) 377, 438
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 1 cut(s) 483
BsoBI CYCGRG 2 cut(s) 99, 441
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 3 cut(s) 304, 468, 493
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 451
BspPI GGATC 2 cut(s) 312, 501
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 2 cut(s) 220, 292
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 2 cut(s) 99, 352
BssMI GATC 3 cut(s) 304, 468, 493
Bst2UI CCWGG 2 cut(s) 353, 454
Bst4CI ACNGT 2 cut(s) 449, 478
Bst6I CTCTTC 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 138
BstF5I GGATG 3 cut(s) 130, 163, 462
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 3 cut(s) 307, 471, 496
BstMAI GTCTC 2 cut(s) 377, 438
BstMBI GATC 3 cut(s) 304, 468, 493
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 2 cut(s) 353, 454
BstSCI CCNGG 4 cut(s) 98, 99, 351, 452
BstSFI CTRYAG 2 cut(s) 201, 477
BstSLI GKGCMC 1 cut(s) 98
BstV1I GCAGC 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 302
BstX2I RGATCY 3 cut(s) 304, 468, 493
BstYI RGATCY 3 cut(s) 304, 468, 493
BsuRI GGCC 1 cut(s) 483
BtrI CACGTC 1 cut(s) 415
BtsCI GGATG 3 cut(s) 130, 163, 462
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 495
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 421
Cfr9I CCCGGG 1 cut(s) 99
CviAII CATG 2 cut(s) 485, 506
CviJI RGCY 2 cut(s) 367, 483
CviKI_1 RGCY 2 cut(s) 367, 483
DdeI CTNAG 1 cut(s) 138
DpnI GATC 3 cut(s) 306, 470, 495
DpnII GATC 3 cut(s) 304, 468, 493
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Eco47III AGCGCT 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 300
Eco88I CYCGRG 2 cut(s) 99, 441
EcoRII CCWGG 2 cut(s) 351, 452
Esp3I CGTCTC 2 cut(s) 377, 438
FaeI CATG 2 cut(s) 488, 509
FaiI YATR 8 cut(s) 26, 107, 183, 193, 203, 234, 486, 507
FaqI GGGAC 1 cut(s) 298
FatI CATG 2 cut(s) 484, 505
Fnu4HI GCNGC 1 cut(s) 419
FokI GGATG 3 cut(s) 137, 170, 469
Fsp4HI GCNGC 1 cut(s) 419
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 419
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 1 cut(s) 483
HapII CCGG 3 cut(s) 100, 155, 422
HhaI GCGC 1 cut(s) 34
Hin1II CATG 2 cut(s) 488, 509
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HpaII CCGG 3 cut(s) 100, 155, 422
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 2 cut(s) 96, 244
Hpy188I TCNGA 3 cut(s) 280, 409, 468
Hpy188III TCNNGA 6 cut(s) 76, 122, 268, 382, 443, 472
Hpy8I GTNNAC 2 cut(s) 96, 244
HpyAV CCTTC 2 cut(s) 275, 355
HpyCH4III ACNGT 2 cut(s) 449, 478
HpyCH4IV ACGT 2 cut(s) 328, 414
HpyCH4V TGCA 3 cut(s) 96, 404, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 2 cut(s) 328, 414
Hsp92II CATG 2 cut(s) 488, 509
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 3 cut(s) 304, 468, 493
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 2 cut(s) 115, 413
MaeI CTAG 1 cut(s) 29
MaeII ACGT 2 cut(s) 328, 414
MalI GATC 3 cut(s) 306, 470, 495
MboI GATC 3 cut(s) 304, 468, 493
MboII GAAGA 2 cut(s) 307, 309
MflI RGATCY 3 cut(s) 304, 468, 493
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 5 cut(s) 66, 88, 272, 333, 511
MnlI CCTC 4 cut(s) 133, 384, 403, 450
MseI TTAA 3 cut(s) 222, 498, 514
MspI CCGG 3 cut(s) 100, 155, 422
MspR9I CCNGG 4 cut(s) 100, 101, 353, 454
MvaI CCWGG 2 cut(s) 353, 454
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 2 cut(s) 100, 101
NdeII GATC 3 cut(s) 304, 468, 493
NlaIII CATG 2 cut(s) 488, 509
NlaIV GGNNCC 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 441
PflMI CCANNNNNTGG 1 cut(s) 490
PfoI TCCNGGA 1 cut(s) 452
PkrI GCNGC 1 cut(s) 420
Psp6I CCWGG 2 cut(s) 351, 452
PspGI CCWGG 2 cut(s) 351, 452
PspN4I GGNNCC 1 cut(s) 451
PsuI RGATCY 3 cut(s) 304, 468, 493
SaqAI TTAA 3 cut(s) 222, 498, 514
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 3 cut(s) 304, 468, 493
ScrFI CCNGG 4 cut(s) 100, 101, 353, 454
SduI GDGCHC 1 cut(s) 98
SetI ASST 5 cut(s) 331, 369, 395, 417, 442
SfaNI GCATC 2 cut(s) 115, 413
SfcI CTRYAG 2 cut(s) 201, 477
Sfr274I CTCGAG 1 cut(s) 441
SlaI CTCGAG 1 cut(s) 441
SmaI CCCGGG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 76, 441
SmoI CTYRAG 2 cut(s) 76, 441
Sse9I AATT 5 cut(s) 66, 88, 272, 333, 511
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 4 cut(s) 98, 99, 351, 452
TaaI ACNGT 2 cut(s) 449, 478
TaiI ACGT 2 cut(s) 331, 417
TaqI TCGA 5 cut(s) 381, 432, 442, 473, 502
TasI AATT 5 cut(s) 66, 88, 272, 333, 511
Tru1I TTAA 3 cut(s) 222, 498, 514
Tru9I TTAA 3 cut(s) 222, 498, 514
TscAI CASTG 1 cut(s) 495
TseI GCWGC 1 cut(s) 418
TspDTI ATGAA 1 cut(s) 72
TspMI CCCGGG 1 cut(s) 99
TspRI CASTG 1 cut(s) 495
Van91I CCANNNNNTGG 1 cut(s) 490
VneI GTGCAC 1 cut(s) 94
XapI RAATTY 1 cut(s) 272
XhoI CTCGAG 1 cut(s) 441
XmaI CCCGGG 1 cut(s) 99
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.