Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015974

Physical Location & Seq

Genomic Coordinates Forward (+)

13165142 .. 13171503

Gene Structure 6362 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1089000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGCTTCTGCGCAGAGCTCCAGAGCTCTCACCCTCCACTCTCTCTCTAACCCACTCCTCCTCCTTCTTCTCCACTCTCTGTTTCTCTAACCCTAATCTCCTCTCCATGCGCTCTTCACTCTTCCCTCCTCTCTCTCTCCTCCGTTCCCTCACTCTCTGTCTCTCTCACAACCAACACCACCACCCTCATACCCATCCATTCTCCAGGCCCATGTCCAGTTTTCCGCTCTCCACCACCAAACCCTTCCGGTTCCACGCCCTTCCCTCTTCCAGTACTTCCGTTGAACACCTTGCAGGCAAGGCCTCGGACCCAGAGGAGAATTGGGATTTGAGCAATGTCGCGCAGAGCGAGGTTTTCGATTTTGAGAATTGCTTTGGTTCCGCCGACTTGAAGCACCTGGGGGCGCCGAAGCTTGAGGTTCCAGAGCTGGAGGAGCTCCCGGAGCAGTGGCGGAGGTCGAAGCTGGCGTGGCTGTGTAAGGAACTGCCGGCGCATAAGGCCGGTACCTTGAGTCGTATACTCAATGCGCAGAAGAAGTGGATAAGGCAAGAAGATGCCACTTATGTTGCCGTGCATTGTATGCGGATTCGGGAAAATGATGTCGGGTTTCGGGTTTACAAATGGATGATGCAACAACACTGGTATCGTTTTGATTTTGCTCTTGCTACTAAGTTAGCGGATTACATGGGTAAAGAGCGAAAATTCTCGAAATGTAGGGATATATTTGATGATATAATTAATCAGGGGCGTGTTCCTAGTGAATCTACATTTCATATATTGGTTGTTGCATATCTTAGTGCCCCGGTTCAAGGTTGCTTGGAGGAAGCATGTGGCATTTACAATCGTATGATTCAGCTAGGAGGTTATCAGCCCCGGCTTCACTTGCACAATTCTCTCTTCAAAGCCCTTGTCAGCCAACCCGGAACCTCTTCGAAACACTACCTTAAACAGGCTGAGTTTATTTTTCATAATTTGGTAACAACTGGACTCGAAATTCACAAGGATATTTATAGTGGATTAATTTGGCTACATAGCTATCAGGATACAATAGATAAAGAAAGGATGGCATTGTTAAGAAAAGAAATGCAACAGGCTGGAATTGAGGAGGGCAGAGATGTGCTTGTGTCTCTCTTGAGAGCTTGCTCAAAAGAGGGGGATGTGGAGGGAGCAGAAAGTACTTGGCTCAGACTTTGCGATCTTGAAGTTGGTCTCCCACCTCAGGCATATGTTTATAAAATGGAAGCCTATTCAAAGGCTGGAGTGCCTTTGAGATCCCTGGAGATATTCAGGGAGATGCAAGAGCAGTTGGGTTCGTCCAACGCTGTCGCATATCATAAAGTCATAGAGGTCTTATGTAAGGCTCAAGAAGTTGAACGTGTCGAGTCCCTCATGACAGAATTCATCAACACTGGTTTGAAGAATTTCATGCCGTCTTATATTGATCTAATGAACATGTACTTCAACTTAGGCTTACATGACAAACTGGAATCAGCCTTCTCCCAGTGCCTTGAGAGATGCCGACCCAGCCGTACTATTTGTTCTATATACTTGGATTCTTTGGTGAAAGTTGGTAATCTTGCTAAAGCTGAGGAGATCTTTGGCCTAATGCAGAGTGATGCGGCAATCGGTGTTAACGCTCGGTCCTGCAACACCATATTAAGTGGATACCTGTCATCTGGAGATTATGTCAAGGCAGAAAACATATTTGACCTGATGTGCCAGAAGAAGTATGACGTTGAACCACCATTGATGGAAAAGATTGATTATATCTTGAGCTTGAGCAGAAAGGTAGTCAAGAAACCTGTAAGCCTGAAGCTGAGCAAAGAACAACGAGAGATTCTAGTAGGTCTTTTGTTGGGTGGTTTGCAGATCGAATCCGATGAAGACTGGAAAAATCACATGATCCGGTTCGAGTTCAGTGAGAGTTCTAGCACACATTCTTTGTTAAAGAGGCATATATTTGATCAATACCATGAGTGGCTACATCCTTCTTGCAAGTCCAGTGAGAGTACGGAGGACATACCATATAAGTTCTCAACCATCTCACATTCGTATTTTGCTTTCTATGCGGAGCAGTTTTGGCCGAAAGGCCGAAGAATGATACCAAAGCTAATCCATCGATGGTTGTCACCATGTGCGCTTGCATACTGGTACATGTACGGGGGCCACAGTACTTCATCAGGAGACATATTGTTGAAGATAAAGGGTAGTGAAGAGGGTGTTGAGAAGATTGTCAAATCCTTAAGGGCAAAGTCCCTTGATTGCAAGGTGAAGAGGAAAGGGAGAGTGTTTTGGATAGGTTTTCTGGGAAGTAGTTCTACCTGGTTCTGGAAATTAGTAGAACCTTATATTCTAGATGACTTGAAGTATGCTCTAAAAGGTGGCCAAATCTCAGATAACAGCATGGTTGAAACCGAAAACGTCAACTTTGGTAGTGGGTCTGATACTAATGAAAATGCTTCTGATTACAGTGATAATGATAATAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015979 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0019684 GO:0022607 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045292 GO:0046483 GO:0048564 GO:0065003 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LAGLIDADG_2 PPR

Protein Analysis

831

Amino Acids

94.81

Weight (kDa)

6.88

Isoelectric Point (pI)

52.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LAGLIDADG_2	PF03161	611 - 776	1.6e-52	LAGLIDADG DNA endonuclease family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15820
fragaria_vesca	FvH4_2g06900
malus_domestica	MD10G1089000.v1.1
prunus_persica	Prupe.8G116000_v2.0.a1
pyrus_communis	pycom10g07190
rosa_chinensis	RchiOBHm_Chr6g0259211
rosa_laevigata	RLG00000014548
rosa_multiflora	Rmu_ssc0000050.1_g000094
rosa_roxburghii	Rroxscaffold_7G00206520
rosa_rugosa	Rorug05G0586000
rosa_samantha	Rh6AG102900 Rh6BG095200 Rh6CG092100 Rh6DG086100
rosa_wichuraiana	Rw6G008880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1233
Acc16I	TGCGCA	2 cut(s)	11, 528
Acc65I	GGTACC	1 cut(s)	503
AccB1I	GGYRCC	2 cut(s)	403, 503
AccBSI	CCGCTC	1 cut(s)	226
AccI	GTMKAC	1 cut(s)	517
AccII	CGCG	1 cut(s)	341
AciI	CCGC	7 cut(s)	224, 381, 451, 583, 677, 1619, 2069
AclWI	GGATC	2 cut(s)	1266, 1897
AcoI	YGGCCR	2 cut(s)	2081, 2383
AcsI	RAATTY	4 cut(s)	701, 993, 1397, 1420
AcuI	CTGAAG	1 cut(s)	1832
AcyI	GRCGYC	1 cut(s)	404
AdeI	CACNNNGTG	1 cut(s)	2135
AfaI	GTAC	9 cut(s)	274, 505, 1177, 1457, 1531, 2011, 2153, 2159, 2173
AfiI	CCNNNNNNNGG	4 cut(s)	809, 949, 1219, 2328
AflII	CTTAAG	1 cut(s)	2242
AflIII	ACRYGT	3 cut(s)	1375, 1452, 2154
AjnI	CCWGG	4 cut(s)	203, 396, 1275, 2321
Alw21I	GWGCWC	3 cut(s)	19, 27, 438
Alw26I	GTCTC	4 cut(s)	164, 1131, 1214, 2178
AlwI	GGATC	2 cut(s)	1266, 1897
AoxI	GGCC	8 cut(s)	206, 300, 498, 1600, 2081, 2089, 2164, 2383
ApoI	RAATTY	4 cut(s)	701, 993, 1397, 1420
ArsI	GACNNNNNNTTYG	2 cut(s)	894, 926
AseI	ATTAAT	2 cut(s)	738, 1019
Asp700I	GAANNNNTTC	3 cut(s)	928, 1421, 2314
Asp718I	GGTACC	1 cut(s)	503
AspLEI	GCGC	7 cut(s)	12, 111, 343, 406, 493, 529, 2140
AspS9I	GGNCC	4 cut(s)	207, 307, 1641, 2164
AsuC2I	CCSGG	4 cut(s)	440, 803, 874, 921
AsuHPI	GGTGA	4 cut(s)	21, 1573, 2121, 2281
AsuII	TTCGAA	1 cut(s)	932
AvaII	GGWCC	2 cut(s)	307, 1641
AxyI	CCTNAGG	1 cut(s)	1218
BaeGI	GKGCMC	1 cut(s)	802
BaeI	ACNNNNGTAYC	2 cut(s)	626, 659
BalI	TGGCCA	1 cut(s)	2385
BanI	GGYRCC	2 cut(s)	403, 503
BanII	GRGCYC	3 cut(s)	19, 27, 438
BarI	GAAGNNNNNNTAC	2 cut(s)	2302, 2334
BbsI	GAAGAC	1 cut(s)	1890
Bbv12I	GWGCWC	3 cut(s)	19, 27, 438
BbvCI	CCTCAGC	1 cut(s)	1587
BccI	CCATC	6 cut(s)	201, 1057, 1744, 2048, 2115, 2124
BceAI	ACGGC	3 cut(s)	554, 1414, 1512
BciT130I	CCWGG	4 cut(s)	205, 398, 1277, 2323
BciVI	GTATCC	2 cut(s)	1036, 1658
BclI	TGATCA	1 cut(s)	1963
BcnI	CCSGG	4 cut(s)	440, 803, 874, 921
BcoDI	GTCTC	4 cut(s)	164, 1131, 1214, 2178
BfaI	CTAG	5 cut(s)	756, 857, 1841, 1929, 2354
BfoI	RGCGCY	1 cut(s)	407
BfrI	CTTAAG	1 cut(s)	2242
BfuI	GTATCC	2 cut(s)	1036, 1658
BglII	AGATCT	1 cut(s)	1593
BisI	GCNGC	1 cut(s)	1620
BlpI	GCTNAGC	1 cut(s)	1817
BlsI	GCNGC	1 cut(s)	1621
BmcAI	AGTACT	3 cut(s)	274, 1177, 2173
Bme1390I	CCNGG	8 cut(s)	205, 398, 440, 803, 874, 921, 1277, 2323
Bme18I	GGWCC	2 cut(s)	307, 1641
BmgT120I	GGNCC	4 cut(s)	207, 307, 1641, 2164
BmiI	GGNNCC	8 cut(s)	251, 309, 379, 405, 420, 505, 925, 2165
BmrFI	CCNGG	8 cut(s)	205, 398, 440, 803, 874, 921, 1277, 2323
BmrI	ACTGGG	1 cut(s)	1495
BmsI	GCATC	5 cut(s)	544, 618, 1284, 1505, 1606
BmuI	ACTGGG	1 cut(s)	1495
BpiI	GAAGAC	1 cut(s)	1890
BpmI	CTGGAG	5 cut(s)	187, 449, 1278, 1298, 1698
Bpu10I	CCTNAGC	1 cut(s)	1587
Bpu1102I	GCTNAGC	1 cut(s)	1817
Bpu14I	TTCGAA	1 cut(s)	932
BpuEI	CTTGAG	7 cut(s)	434, 529, 1153, 1347, 1529, 1792, 1798
BpuMI	CCSGG	4 cut(s)	440, 803, 874, 921
Bsa29I	ATCGAT	1 cut(s)	2119
BsaHI	GRCGYC	1 cut(s)	404
BsaI	GGTCTC	1 cut(s)	1214
BsaJI	CCNNGG	5 cut(s)	303, 397, 801, 872, 1275
BsaWI	WCCGGW	2 cut(s)	246, 1905
BsaXI	ACNNNNNCTCC	4 cut(s)	44, 74, 1671, 1701
Bsc4I	CCNNNNNNNGG	4 cut(s)	809, 949, 1219, 2328
Bse118I	RCCGGY	2 cut(s)	487, 500
Bse21I	CCTNAGG	1 cut(s)	1218
Bse3DI	GCAATG	1 cut(s)	340
BseBI	CCWGG	4 cut(s)	205, 398, 1277, 2323
BseCI	ATCGAT	1 cut(s)	2119
BseDI	CCNNGG	5 cut(s)	303, 397, 801, 872, 1275
BseGI	GGATG	5 cut(s)	193, 630, 1068, 1162, 1984
BseLI	CCNNNNNNNGG	4 cut(s)	809, 949, 1219, 2328
BseMI	GCAATG	1 cut(s)	340
BseMII	CTCAG	6 cut(s)	945, 1198, 1232, 1578, 1808, 2406
BseRI	GAGGAG	9 cut(s)	46, 49, 89, 117, 128, 329, 446, 1118, 1604
BseSI	GKGCMC	1 cut(s)	802
BseYI	CCCAGC	1 cut(s)	1523
Bsh1236I	CGCG	1 cut(s)	341
BshFI	GGCC	8 cut(s)	208, 302, 500, 1602, 2083, 2091, 2166, 2385
BshNI	GGYRCC	2 cut(s)	403, 503
BshVI	ATCGAT	1 cut(s)	2119
BsiHKAI	GWGCWC	3 cut(s)	19, 27, 438
BsiSI	CCGG	8 cut(s)	247, 440, 488, 501, 803, 874, 921, 1906
BslFI	GGGAC	2 cut(s)	1369, 2239
BslI	CCNNNNNNNGG	4 cut(s)	809, 949, 1219, 2328
BsmAI	GTCTC	4 cut(s)	164, 1131, 1214, 2178
BsmFI	GGGAC	2 cut(s)	1369, 2239
BsnI	GGCC	8 cut(s)	208, 302, 500, 1602, 2083, 2091, 2166, 2385
Bso31I	GGTCTC	1 cut(s)	1214
Bsp119I	TTCGAA	1 cut(s)	932
Bsp1286I	GDGCHC	4 cut(s)	19, 27, 438, 802
Bsp143I	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
Bsp1720I	GCTNAGC	1 cut(s)	1817
BspACI	CCGC	7 cut(s)	224, 381, 451, 583, 677, 1619, 2069
BspANI	GGCC	8 cut(s)	208, 302, 500, 1602, 2083, 2091, 2166, 2385
BspCNI	CTCAG	6 cut(s)	946, 1197, 1231, 1579, 1809, 2405
BspDI	ATCGAT	1 cut(s)	2119
BspFNI	CGCG	1 cut(s)	341
BspHI	TCATGA	1 cut(s)	1389
BspLI	GGNNCC	8 cut(s)	251, 309, 379, 405, 420, 505, 925, 2165
BspPI	GGATC	2 cut(s)	1266, 1897
BspQI	GCTCTTC	1 cut(s)	118
BspT104I	TTCGAA	1 cut(s)	932
BspT107I	GGYRCC	2 cut(s)	403, 503
BspTI	CTTAAG	1 cut(s)	2242
BspTNI	GGTCTC	1 cut(s)	1214
BsrBI	CCGCTC	1 cut(s)	226
BsrDI	GCAATG	1 cut(s)	340
BsrFI	RCCGGY	2 cut(s)	487, 500
BssAI	RCCGGY	2 cut(s)	487, 500
BssECI	CCNNGG	5 cut(s)	303, 397, 801, 872, 1275
BssMI	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
BssNAI	GTATAC	1 cut(s)	518
BssNI	GRCGYC	1 cut(s)	404
Bst1107I	GTATAC	1 cut(s)	518
Bst2UI	CCWGG	4 cut(s)	205, 398, 1277, 2323
Bst4CI	ACNGT	2 cut(s)	2171, 2471
Bst6I	CTCTTC	7 cut(s)	118, 125, 271, 902, 934, 2208, 2267
BstACI	GRCGYC	1 cut(s)	404
BstAFI	CTTAAG	1 cut(s)	2242
BstAPI	GCANNNNNTGC	1 cut(s)	580
BstBI	TTCGAA	1 cut(s)	932
BstC8I	GCNNGC	5 cut(s)	295, 465, 489, 1141, 2142
BstDEI	CTNAG	9 cut(s)	669, 794, 954, 1184, 1218, 1465, 1587, 1817, 2392
BstENI	CCTNNNNNAGG	1 cut(s)	947
BstF5I	GGATG	5 cut(s)	193, 630, 1068, 1162, 1984
BstFNI	CGCG	1 cut(s)	341
BstH2I	RGCGCY	1 cut(s)	407
BstHHI	GCGC	7 cut(s)	12, 111, 343, 406, 493, 529, 2140
BstKTI	GATC	7 cut(s)	1198, 1274, 1444, 1596, 1872, 1905, 1966
BstMAI	GTCTC	4 cut(s)	164, 1131, 1214, 2178
BstMBI	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
BstNI	CCWGG	4 cut(s)	205, 398, 1277, 2323
BstNSI	RCATGY	3 cut(s)	831, 1456, 2158
BstSCI	CCNGG	8 cut(s)	203, 396, 438, 801, 872, 919, 1275, 2321
BstSLI	GKGCMC	1 cut(s)	802
BstUI	CGCG	1 cut(s)	341
BstV2I	GAAGAC	1 cut(s)	1890
BstX2I	RGATCY	2 cut(s)	1271, 1593
BstYI	RGATCY	2 cut(s)	1271, 1593
BstZ17I	GTATAC	1 cut(s)	518
Bsu15I	ATCGAT	1 cut(s)	2119
Bsu36I	CCTNAGG	1 cut(s)	1218
BsuI	GTATCC	2 cut(s)	1036, 1658
BsuRI	GGCC	8 cut(s)	208, 302, 500, 1602, 2083, 2091, 2166, 2385
BsuTUI	ATCGAT	1 cut(s)	2119
BtsCI	GGATG	5 cut(s)	193, 630, 1068, 1162, 1984
BtsI	GCAGTG	1 cut(s)	452
BtsIMutI	CAGTG	7 cut(s)	452, 637, 1407, 1508, 1924, 2008, 2476
Cac8I	GCNNGC	5 cut(s)	295, 465, 489, 1141, 2142
CciI	TCATGA	1 cut(s)	1389
CfoI	GCGC	7 cut(s)	12, 111, 343, 406, 493, 529, 2140
Cfr10I	RCCGGY	2 cut(s)	487, 500
Cfr13I	GGNCC	4 cut(s)	207, 307, 1641, 2164
ClaI	ATCGAT	1 cut(s)	2119
CsiI	ACCWGGT	1 cut(s)	2321
Csp6I	GTAC	9 cut(s)	273, 504, 1176, 1456, 1530, 2010, 2152, 2158, 2172
CspCI	CAANNNNNGTGG	2 cut(s)	547, 582
CviQI	GTAC	9 cut(s)	273, 504, 1176, 1456, 1530, 2010, 2152, 2158, 2172
DdeI	CTNAG	9 cut(s)	669, 794, 954, 1184, 1218, 1465, 1587, 1817, 2392
DinI	GGCGCC	1 cut(s)	405
DpnI	GATC	7 cut(s)	1197, 1273, 1443, 1595, 1871, 1904, 1965
DpnII	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
DraIII	CACNNNGTG	1 cut(s)	2135
EaeI	YGGCCR	2 cut(s)	2081, 2383
Eam1104I	CTCTTC	7 cut(s)	118, 125, 271, 902, 934, 2208, 2267
EarI	CTCTTC	7 cut(s)	118, 125, 271, 902, 934, 2208, 2267
EciI	GGCGGA	2 cut(s)	370, 466
Ecl136II	GAGCTC	3 cut(s)	17, 25, 436
Eco147I	AGGCCT	1 cut(s)	302
Eco24I	GRGCYC	3 cut(s)	19, 27, 438
Eco31I	GGTCTC	1 cut(s)	1214
Eco47I	GGWCC	2 cut(s)	307, 1641
Eco53kI	GAGCTC	3 cut(s)	17, 25, 436
Eco57I	CTGAAG	1 cut(s)	1832
Eco81I	CCTNAGG	1 cut(s)	1218
EcoICRI	GAGCTC	3 cut(s)	17, 25, 436
EcoNI	CCTNNNNNAGG	1 cut(s)	947
EcoRI	GAATTC	1 cut(s)	1397
EcoRII	CCWGG	4 cut(s)	203, 396, 1275, 2321
EcoT38I	GRGCYC	3 cut(s)	19, 27, 438
EgeI	GGCGCC	1 cut(s)	405
EheI	GGCGCC	1 cut(s)	405
FaqI	GGGAC	2 cut(s)	1369, 2239
FauNDI	CATATG	1 cut(s)	1225
FbaI	TGATCA	1 cut(s)	1963
FblI	GTMKAC	1 cut(s)	517
Fnu4HI	GCNGC	1 cut(s)	1620
FokI	GGATG	5 cut(s)	180, 637, 1075, 1169, 1971
FriOI	GRGCYC	3 cut(s)	19, 27, 438
Fsp4HI	GCNGC	1 cut(s)	1620
FspBI	CTAG	5 cut(s)	756, 857, 1841, 1929, 2354
FspI	TGCGCA	2 cut(s)	11, 528
GlaI	GCGC	7 cut(s)	11, 110, 342, 405, 492, 528, 2139
GluI	GCNGC	1 cut(s)	1620
GsaI	CCCAGC	1 cut(s)	1527
GsuI	CTGGAG	5 cut(s)	187, 449, 1278, 1298, 1698
HaeII	RGCGCY	1 cut(s)	407
HaeIII	GGCC	8 cut(s)	208, 302, 500, 1602, 2083, 2091, 2166, 2385
HapII	CCGG	8 cut(s)	247, 440, 488, 501, 803, 874, 921, 1906
HhaI	GCGC	7 cut(s)	12, 111, 343, 406, 493, 529, 2140
Hin1I	GRCGYC	1 cut(s)	404
Hin6I	GCGC	7 cut(s)	10, 109, 341, 404, 491, 527, 2138
HinP1I	GCGC	7 cut(s)	10, 109, 341, 404, 491, 527, 2138
HincII	GTYRAC	2 cut(s)	1633, 2425
HindII	GTYRAC	2 cut(s)	1633, 2425
HindIII	AAGCTT	1 cut(s)	410
HpaI	GTTAAC	1 cut(s)	1633
HpaII	CCGG	8 cut(s)	247, 440, 488, 501, 803, 874, 921, 1906
HphI	GGTGA	4 cut(s)	21, 1573, 2121, 2281
Hpy166II	GTNNAC	4 cut(s)	518, 616, 1633, 2425
Hpy188I	TCNGA	6 cut(s)	307, 1187, 1879, 2395, 2443, 2464
Hpy8I	GTNNAC	4 cut(s)	518, 616, 1633, 2425
HpyAV	CCTTC	5 cut(s)	73, 253, 269, 1504, 1998
HpyCH4III	ACNGT	2 cut(s)	2171, 2471
HpyCH4IV	ACGT	3 cut(s)	1375, 1734, 2421
HpyF3I	CTNAG	9 cut(s)	669, 794, 954, 1184, 1218, 1465, 1587, 1817, 2392
HpySE526I	ACGT	3 cut(s)	1375, 1734, 2421
Hsp92I	GRCGYC	1 cut(s)	404
HspAI	GCGC	7 cut(s)	10, 109, 341, 404, 491, 527, 2138
KasI	GGCGCC	1 cut(s)	403
KpnI	GGTACC	1 cut(s)	507
KroI	GCCGGC	1 cut(s)	487
KroNI	GCCGGC	1 cut(s)	489
Ksp22I	TGATCA	1 cut(s)	1963
KspAI	GTTAAC	1 cut(s)	1633
Kzo9I	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
LguI	GCTCTTC	1 cut(s)	118
LmnI	GCTCC	6 cut(s)	22, 433, 441, 442, 1166, 2071
LweI	GCATC	5 cut(s)	544, 618, 1284, 1505, 1606
MabI	ACCWGGT	1 cut(s)	2321
MaeI	CTAG	5 cut(s)	756, 857, 1841, 1929, 2354
MaeII	ACGT	3 cut(s)	1375, 1734, 2421
MaeIII	GTNAC	2 cut(s)	976, 2127
MalI	GATC	7 cut(s)	1197, 1273, 1443, 1595, 1871, 1904, 1965
MbiI	CCGCTC	1 cut(s)	226
MboI	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
MflI	RGATCY	2 cut(s)	1271, 1593
MhlI	GDGCHC	4 cut(s)	19, 27, 438, 802
MlsI	TGGCCA	1 cut(s)	2385
MluNI	TGGCCA	1 cut(s)	2385
Mly113I	GGCGCC	1 cut(s)	404
MlyI	GAGTC	3 cut(s)	520, 981, 1391
MmeI	TCCRAC	1 cut(s)	1341
Mox20I	TGGCCA	1 cut(s)	2385
MroNI	GCCGGC	1 cut(s)	487
MroXI	GAANNNNTTC	3 cut(s)	928, 1421, 2314
MscI	TGGCCA	1 cut(s)	2385
MseI	TTAA	8 cut(s)	738, 945, 1019, 1073, 1632, 1658, 1946, 2243
Msp20I	TGGCCA	1 cut(s)	2385
MspCI	CTTAAG	1 cut(s)	2242
MspI	CCGG	8 cut(s)	247, 440, 488, 501, 803, 874, 921, 1906
MspR9I	CCNGG	8 cut(s)	205, 398, 440, 803, 874, 921, 1277, 2323
MvaI	CCWGG	4 cut(s)	205, 398, 1277, 2323
MvnI	CGCG	1 cut(s)	341
NaeI	GCCGGC	1 cut(s)	489
NarI	GGCGCC	1 cut(s)	404
NciI	CCSGG	4 cut(s)	440, 803, 874, 921
NdeI	CATATG	1 cut(s)	1225
NdeII	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
NgoMIV	GCCGGC	1 cut(s)	487
NlaIV	GGNNCC	8 cut(s)	251, 309, 379, 405, 420, 505, 925, 2165
NmuCI	GTSAC	1 cut(s)	2127
NsbI	TGCGCA	2 cut(s)	11, 528
NspI	RCATGY	3 cut(s)	831, 1456, 2158
NspV	TTCGAA	1 cut(s)	932
PagI	TCATGA	1 cut(s)	1389
PceI	AGGCCT	1 cut(s)	302
PciI	ACATGT	2 cut(s)	1452, 2154
PciSI	GCTCTTC	1 cut(s)	118
PcsI	WCGNNNNNNNCGW	2 cut(s)	345, 464
PdiI	GCCGGC	1 cut(s)	489
PdmI	GAANNNNTTC	3 cut(s)	928, 1421, 2314
PfeI	GAWTC	7 cut(s)	586, 761, 850, 1487, 1553, 1837, 1874
PfoI	TCCNGGA	1 cut(s)	438
PkrI	GCNGC	1 cut(s)	1621
PleI	GAGTC	3 cut(s)	519, 981, 1390
PluTI	GGCGCC	1 cut(s)	407
PpsI	GAGTC	3 cut(s)	519, 981, 1390
PscI	ACATGT	2 cut(s)	1452, 2154
PshBI	ATTAAT	2 cut(s)	738, 1019
PsiI	TTATAA	1 cut(s)	1233
Psp124BI	GAGCTC	3 cut(s)	19, 27, 438
Psp6I	CCWGG	4 cut(s)	203, 396, 1275, 2321
PspFI	CCCAGC	1 cut(s)	1523
PspGI	CCWGG	4 cut(s)	203, 396, 1275, 2321
PspN4I	GGNNCC	8 cut(s)	251, 309, 379, 405, 420, 505, 925, 2165
PspPI	GGNCC	4 cut(s)	207, 307, 1641, 2164
PsrI	GAACNNNNNNTAC	2 cut(s)	1522, 1554
PsuI	RGATCY	2 cut(s)	1271, 1593
RsaI	GTAC	9 cut(s)	274, 505, 1177, 1457, 1531, 2011, 2153, 2159, 2173
RsaNI	GTAC	9 cut(s)	273, 504, 1176, 1456, 1530, 2010, 2152, 2158, 2172
SacI	GAGCTC	3 cut(s)	19, 27, 438
SapI	GCTCTTC	1 cut(s)	118
SaqAI	TTAA	8 cut(s)	738, 945, 1019, 1073, 1632, 1658, 1946, 2243
SatI	GCNGC	1 cut(s)	1620
Sau3AI	GATC	7 cut(s)	1195, 1271, 1441, 1593, 1869, 1902, 1963
Sau96I	GGNCC	4 cut(s)	207, 307, 1641, 2164
ScaI	AGTACT	3 cut(s)	274, 1177, 2173
SchI	GAGTC	3 cut(s)	520, 981, 1391
ScrFI	CCNGG	8 cut(s)	205, 398, 440, 803, 874, 921, 1277, 2323
SduI	GDGCHC	4 cut(s)	19, 27, 438, 802
SexAI	ACCWGGT	1 cut(s)	2321
SfaNI	GCATC	5 cut(s)	544, 618, 1284, 1505, 1606
SfoI	GGCGCC	1 cut(s)	405
SfuI	TTCGAA	1 cut(s)	932
SinI	GGWCC	2 cut(s)	307, 1641
SmlI	CTYRAG	8 cut(s)	413, 508, 1132, 1362, 1508, 1771, 1777, 2242
SmoI	CTYRAG	8 cut(s)	413, 508, 1132, 1362, 1508, 1771, 1777, 2242
SseBI	AGGCCT	1 cut(s)	302
SsiI	CCGC	7 cut(s)	224, 381, 451, 583, 677, 1619, 2069
SspDI	GGCGCC	1 cut(s)	403
SspMI	CTAG	5 cut(s)	756, 857, 1841, 1929, 2354
SstI	GAGCTC	3 cut(s)	19, 27, 438
StuI	AGGCCT	1 cut(s)	302
StyD4I	CCNGG	8 cut(s)	203, 396, 438, 801, 872, 919, 1275, 2321
TaaI	ACNGT	2 cut(s)	2171, 2471
TaiI	ACGT	3 cut(s)	1378, 1737, 2424
TaqI	TCGA	9 cut(s)	357, 458, 707, 932, 990, 1380, 1872, 1911, 2119
TaqII	GACCGA	1 cut(s)	1629
TatI	WGTACW	4 cut(s)	272, 1175, 1455, 2171
TauI	GCSGC	1 cut(s)	1622
TfiI	GAWTC	7 cut(s)	586, 761, 850, 1487, 1553, 1837, 1874
Tru1I	TTAA	8 cut(s)	738, 945, 1019, 1073, 1632, 1658, 1946, 2243
Tru9I	TTAA	8 cut(s)	738, 945, 1019, 1073, 1632, 1658, 1946, 2243
TscAI	CASTG	7 cut(s)	452, 644, 1414, 1508, 1924, 2008, 2476
TseFI	GTSAC	1 cut(s)	2127
Tsp45I	GTSAC	1 cut(s)	2127
TspDTI	ATGAA	8 cut(s)	761, 956, 1390, 1414, 1463, 1896, 2166, 2466
TspGWI	ACGGA	3 cut(s)	131, 268, 2027
TspRI	CASTG	7 cut(s)	452, 644, 1414, 1508, 1924, 2008, 2476
Vha464I	CTTAAG	1 cut(s)	2242
VpaK11BI	GGWCC	2 cut(s)	307, 1641
VspI	ATTAAT	2 cut(s)	738, 1019
XagI	CCTNNNNNAGG	1 cut(s)	947
XapI	RAATTY	4 cut(s)	701, 993, 1397, 1420
XbaI	TCTAGA	1 cut(s)	2353
XceI	RCATGY	3 cut(s)	831, 1456, 2158
XcmI	CCANNNNNNNNNTGG	1 cut(s)	318
XmiI	GTMKAC	1 cut(s)	517
XmnI	GAANNNNTTC	3 cut(s)	928, 1421, 2314
XspI	CTAG	5 cut(s)	756, 857, 1841, 1929, 2354
ZrmI	AGTACT	3 cut(s)	274, 1177, 2173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.