Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0015974

Physical Location & Seq

Genomic Coordinates Forward (+)

8399440 .. 8402106

Gene Structure 2667 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g07190.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGCTTCTGTGCAGAGCTCCAGAGCTCTCACCCTCCACTCTCTCTCTAATCCACTCCTCCTTCTTCTCCACTATCTGTTTCTCTAACCCTAATCTCCTCTCCATGCGCTCTTCACTCTTCCCTCCTCTCTCTCTCCTCCGCTCCCTCACTCTCTGTCTCTCTCACAACCAACACCACCACCCTCCTACCCACCCATTCTCCAGGCACATATCCAGTTTTCCGCTCTCCACCACCAAACCCTGCCGGTTCCACGCCCTTCCCTCTTCCAGTACTTCCGTTGAACACGTTGCAGGCAAGGCCTCGGACCCAGAGGAGAATTGGGATTTGAGCAATGTCGCGCAGAGCGAGGTTTTCGATGTTGAGAAGTGCTTTGCTTCCGCCGACTTGAAGCACCTGGGGGCGCCGAAGCTTGAGGTTTCAGAGCTGGAGGAGCTCCCGGAGCAGTGGCGGAGGTCGAAGCTGGCGTGGCTGTGTAAGGAACTGCCGGCGCATAAGGCCGGTACCTTGAGTCGTATACTCAATGCGCAGAAGAAATGGATAAGGCAAGAAGATGCCACTTATGTTGCCGTGCATTGTATGCGGATTCGGGAAAATGATGTCGGGTTTCGGGTTTACAAATGGATGATGCAACAACACTGGTATCGTTTTGATTTTGCTCTTGCTACTAAGTTAGCGGATTACATGGGTAAAGAGCGAAAATTCTCGAAATGTAGGGATATATTTGATGATACAATTAATCAGGGGCGTGTTCCTAGCGAATCTACATTTCATATATTGGTTGTAGCATATCTCAGTGCCCCAGTTCAAGGTTGCTTGGAGGAAGCATGTGGCATTTACAATCGTATGATTCAGCTAGGAGGTTATCAGCCCCGGCTTCACTTGCACAATTCTCTCTTCAAAGCCCTTGTCAGCCAACCTGGAACCTCTTCGAAACACTACCTTAAACAGGCTGAGTTTATTTTTCATAATTTGGTAACAACTGGACTCGAAATTCACAAGGATATTTATAGTGGATTAATTTGGCTACATAGCTATCAGGATACAATAGATAAAGAGAGGGTGGCATTGTTAAGAAAAGAAATGCAACAGGCTGGAATTGAGGAGGGCAGAGATGTGCTTGTGTCTCTCTTGAGAGCTTGCTCAAAAGAGGGGGACGTGGAGGGAGCAGAAAGTACTTGGCTCAGACTTTGCGATCTTGAAGTTGGTCTCCCACCTCAGGCATATGTTTATAAAATGGAAGCCTATTCAAGGGCTGGAGTGCCTTTGAGAGCCCTAGAGATATTCAGGGAGATGCAAGAGCAGTTGGGTTCGTCCAATGCTGTCGCATATCATAAAGTCATAGAGGTCTTATGTAAGGCTCAAGAAGTTGAACGTGTCGAGTCCCTCATGACAGAATTCATCAACACTGGTTTGAAGAATTTCATGCCGTCTTATATTGATCTAATGAACATGTACTTCAACTTAGGCTTACATGACAAACTGGAATCAGCCTTCTCTCAGTGCCTTGAGAGATGCCGACCCAGCCGTACTATTTGTTCTATATACTTGGATTCTTTGGTGAAAGTTGGTAATCTTGCTAGAGCTGAGGAGATCTTTGGCTTAATGCAGAGTGATGCGGCAATCGGTGTTAATGCTCGGTCCTGCAACACCATATTAAGTGGATACCTGTCATCTGGAGATTATGTCAAGGCAGAAAACATATTTGACCTGATGTGCCAGAAGAAGTATGACGTTGAACCACCATTGATGGAAAATATTGATTATATCTTGAGCTTGAGCAGAAAGGTGGTCAAGAAACCTGTAAGCCTGAAGCTGAGCAAAGAACAACGAGAGATTCTAGTAGGTCTTTTGTTGGGTGGTTTGCAGATCGAATCTGATGAAGACTGGAAAAATCACATGATCCGGTTTGAGTTCAGTGAGAATTCTAGCACACATTCTTTGTTAAAGAGGCATATATTTGATCAATACCATGAGTGGCTACATCCTTCTTGCAAGTCCAGCGAGAGTACAGAGGACATACCATATAAGTTCTCAACCATCTCACATTCGTATTTTGCTTTCTATGCGGAGCAGTTTTGGCCAAAAGGCCGAAGAATGATACCAAAGCTAATCCATCGATGGTTGTCACCATGTGCGCTTGCATACTGGTACATGTACGGGGGCCACAAGACTTCATCAGGAGACGTATTGTTGAAGATAAAGGGTAGTGAGGAGGGTGTTGAGAAGATTGTCAAATCCTTAAAGGCAAAGTCCCTGGATTGCAAGGTGAAGAGGAAAGGGAGAGTGTTTTGGATAGGTTTTCTGGGAAGTAGTTCTACCTGGTTCTGGAAATTAGTAGAACCTTATATTCTAGATGACTTGAAGTATGCTCTAAAAGGTGGCCAAATCTCAGATAACAGCAGGGTTGAAACCGAAAACGTCAACTTTGGTAGTGGGTCTGATACTAATGAAAATGCTTCTGATTACAGTGATGATGATAATAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015979 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0019684 GO:0022607 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045292 GO:0046483 GO:0048564 GO:0065003 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LAGLIDADG_2 PPR

Protein Analysis

830

Amino Acids

94.61

Weight (kDa)

6.88

Isoelectric Point (pI)

50.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LAGLIDADG_2	PF03161	610 - 775	8.3e-53	LAGLIDADG DNA endonuclease family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15820
fragaria_vesca	FvH4_2g06900
malus_domestica	MD10G1089000.v1.1
prunus_persica	Prupe.8G116000_v2.0.a1
pyrus_communis	pycom10g07190
rosa_chinensis	RchiOBHm_Chr6g0259211
rosa_laevigata	RLG00000014548
rosa_multiflora	Rmu_ssc0000050.1_g000094
rosa_roxburghii	Rroxscaffold_7G00206520
rosa_rugosa	Rorug05G0586000
rosa_samantha	Rh6AG102900 Rh6BG095200 Rh6CG092100 Rh6DG086100
rosa_wichuraiana	Rw6G008880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1230
Acc16I	TGCGCA	1 cut(s)	525
Acc65I	GGTACC	1 cut(s)	500
AccB1I	GGYRCC	2 cut(s)	400, 500
AccBSI	CCGCTC	2 cut(s)	141, 223
AccI	GTMKAC	1 cut(s)	514
AccII	CGCG	1 cut(s)	338
AciI	CCGC	8 cut(s)	139, 221, 378, 448, 580, 674, 1616, 2066
AclWI	GGATC	1 cut(s)	1894
AcoI	YGGCCR	2 cut(s)	2078, 2380
AcsI	RAATTY	5 cut(s)	698, 990, 1394, 1417, 1921
AcuI	CTGAAG	1 cut(s)	1829
AcyI	GRCGYC	1 cut(s)	401
AdeI	CACNNNGTG	1 cut(s)	2132
AfaI	GTAC	8 cut(s)	271, 502, 1174, 1454, 1528, 2008, 2150, 2156
AfiI	CCNNNNNNNGG	5 cut(s)	806, 946, 1216, 1248, 2325
AflIII	ACRYGT	4 cut(s)	283, 1372, 1449, 2151
AjiI	CACGTC	1 cut(s)	1156
AjnI	CCWGG	5 cut(s)	200, 393, 916, 2253, 2318
Alw21I	GWGCWC	3 cut(s)	19, 27, 435
Alw26I	GTCTC	4 cut(s)	161, 1128, 1211, 2175
AlwI	GGATC	1 cut(s)	1894
AoxI	GGCC	6 cut(s)	297, 495, 2078, 2086, 2161, 2380
ApoI	RAATTY	5 cut(s)	698, 990, 1394, 1417, 1921
ArsI	GACNNNNNNTTYG	2 cut(s)	891, 923
AseI	ATTAAT	2 cut(s)	735, 1016
Asp700I	GAANNNNTTC	3 cut(s)	925, 1418, 2311
Asp718I	GGTACC	1 cut(s)	500
AspLEI	GCGC	6 cut(s)	108, 340, 403, 490, 526, 2137
AspS9I	GGNCC	3 cut(s)	304, 1638, 2161
AsuC2I	CCSGG	2 cut(s)	437, 871
AsuHPI	GGTGA	4 cut(s)	21, 1570, 2118, 2278
AsuII	TTCGAA	1 cut(s)	929
AvaII	GGWCC	2 cut(s)	304, 1638
AxyI	CCTNAGG	1 cut(s)	1215
BaeGI	GKGCMC	1 cut(s)	799
BaeI	ACNNNNGTAYC	2 cut(s)	623, 656
BalI	TGGCCA	2 cut(s)	2080, 2382
BanI	GGYRCC	2 cut(s)	400, 500
BanII	GRGCYC	4 cut(s)	19, 27, 435, 1273
BarI	GAAGNNNNNNTAC	2 cut(s)	2299, 2331
BbsI	GAAGAC	1 cut(s)	1887
Bbv12I	GWGCWC	3 cut(s)	19, 27, 435
BbvCI	CCTCAGC	1 cut(s)	1584
BccI	CCATC	4 cut(s)	1741, 2045, 2112, 2121
BceAI	ACGGC	3 cut(s)	551, 1411, 1509
BciT130I	CCWGG	5 cut(s)	202, 395, 918, 2255, 2320
BciVI	GTATCC	2 cut(s)	1033, 1655
BclI	TGATCA	1 cut(s)	1960
BcnI	CCSGG	2 cut(s)	437, 871
BcoDI	GTCTC	4 cut(s)	161, 1128, 1211, 2175
BfaI	CTAG	7 cut(s)	753, 854, 1274, 1578, 1838, 1926, 2351
BfoI	RGCGCY	1 cut(s)	404
BfuI	GTATCC	2 cut(s)	1033, 1655
BglII	AGATCT	1 cut(s)	1590
BisI	GCNGC	1 cut(s)	1617
BlpI	GCTNAGC	1 cut(s)	1814
BlsI	GCNGC	1 cut(s)	1618
BmcAI	AGTACT	2 cut(s)	271, 1174
Bme1390I	CCNGG	7 cut(s)	202, 395, 437, 871, 918, 2255, 2320
Bme18I	GGWCC	2 cut(s)	304, 1638
BmgBI	CACGTC	1 cut(s)	1156
BmgT120I	GGNCC	3 cut(s)	304, 1638, 2161
BmiI	GGNNCC	6 cut(s)	248, 306, 402, 502, 922, 2162
BmrFI	CCNGG	7 cut(s)	202, 395, 437, 871, 918, 2255, 2320
BmrI	ACTGGG	1 cut(s)	794
BmsI	GCATC	5 cut(s)	541, 615, 1281, 1502, 1603
BmuI	ACTGGG	1 cut(s)	794
BpiI	GAAGAC	1 cut(s)	1887
BpmI	CTGGAG	4 cut(s)	184, 446, 1275, 1695
Bpu10I	CCTNAGC	1 cut(s)	1584
Bpu1102I	GCTNAGC	1 cut(s)	1814
Bpu14I	TTCGAA	1 cut(s)	929
BpuEI	CTTGAG	7 cut(s)	431, 526, 1150, 1344, 1526, 1789, 1795
BpuMI	CCSGG	2 cut(s)	437, 871
Bsa29I	ATCGAT	1 cut(s)	2116
BsaHI	GRCGYC	1 cut(s)	401
BsaI	GGTCTC	1 cut(s)	1211
BsaJI	CCNNGG	4 cut(s)	300, 394, 869, 2253
BsaWI	WCCGGW	1 cut(s)	1902
BsaXI	ACNNNNNCTCC	4 cut(s)	182, 212, 1668, 1698
Bsc4I	CCNNNNNNNGG	5 cut(s)	806, 946, 1216, 1248, 2325
Bse118I	RCCGGY	3 cut(s)	243, 484, 497
Bse1I	ACTGG	9 cut(s)	213, 267, 641, 800, 985, 1411, 1485, 1889, 2150
Bse21I	CCTNAGG	1 cut(s)	1215
Bse3DI	GCAATG	1 cut(s)	337
BseBI	CCWGG	5 cut(s)	202, 395, 918, 2255, 2320
BseCI	ATCGAT	1 cut(s)	2116
BseDI	CCNNGG	4 cut(s)	300, 394, 869, 2253
BseGI	GGATG	2 cut(s)	627, 1981
BseLI	CCNNNNNNNGG	5 cut(s)	806, 946, 1216, 1248, 2325
BseMI	GCAATG	1 cut(s)	337
BseMII	CTCAG	8 cut(s)	805, 942, 1195, 1229, 1511, 1575, 1805, 2403
BseNI	ACTGG	9 cut(s)	213, 267, 641, 800, 985, 1411, 1485, 1889, 2150
BseRI	GAGGAG	9 cut(s)	46, 86, 114, 125, 326, 443, 1115, 1601, 2225
BseSI	GKGCMC	1 cut(s)	799
BseYI	CCCAGC	1 cut(s)	1520
BsgI	GTGCAG	1 cut(s)	31
Bsh1236I	CGCG	1 cut(s)	338
BshFI	GGCC	6 cut(s)	299, 497, 2080, 2088, 2163, 2382
BshNI	GGYRCC	2 cut(s)	400, 500
BshVI	ATCGAT	1 cut(s)	2116
BsiHKAI	GWGCWC	3 cut(s)	19, 27, 435
BsiSI	CCGG	6 cut(s)	244, 437, 485, 498, 871, 1903
BslFI	GGGAC	3 cut(s)	1166, 1366, 2236
BslI	CCNNNNNNNGG	5 cut(s)	806, 946, 1216, 1248, 2325
BsmAI	GTCTC	4 cut(s)	161, 1128, 1211, 2175
BsmBI	CGTCTC	1 cut(s)	2175
BsmFI	GGGAC	3 cut(s)	1166, 1366, 2236
BsnI	GGCC	6 cut(s)	299, 497, 2080, 2088, 2163, 2382
Bso31I	GGTCTC	1 cut(s)	1211
Bsp119I	TTCGAA	1 cut(s)	929
Bsp1286I	GDGCHC	5 cut(s)	19, 27, 435, 799, 1273
Bsp143I	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
Bsp1720I	GCTNAGC	1 cut(s)	1814
BspACI	CCGC	8 cut(s)	139, 221, 378, 448, 580, 674, 1616, 2066
BspANI	GGCC	6 cut(s)	299, 497, 2080, 2088, 2163, 2382
BspCNI	CTCAG	8 cut(s)	804, 943, 1194, 1228, 1510, 1576, 1806, 2402
BspDI	ATCGAT	1 cut(s)	2116
BspFNI	CGCG	1 cut(s)	338
BspHI	TCATGA	1 cut(s)	1386
BspLI	GGNNCC	6 cut(s)	248, 306, 402, 502, 922, 2162
BspPI	GGATC	1 cut(s)	1894
BspQI	GCTCTTC	1 cut(s)	115
BspT104I	TTCGAA	1 cut(s)	929
BspT107I	GGYRCC	2 cut(s)	400, 500
BspTNI	GGTCTC	1 cut(s)	1211
BsrBI	CCGCTC	2 cut(s)	141, 223
BsrDI	GCAATG	1 cut(s)	337
BsrFI	RCCGGY	3 cut(s)	243, 484, 497
BsrI	ACTGG	9 cut(s)	213, 267, 641, 800, 985, 1411, 1485, 1889, 2150
BssAI	RCCGGY	3 cut(s)	243, 484, 497
BssECI	CCNNGG	4 cut(s)	300, 394, 869, 2253
BssMI	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
BssNAI	GTATAC	1 cut(s)	515
BssNI	GRCGYC	1 cut(s)	401
Bst1107I	GTATAC	1 cut(s)	515
Bst2UI	CCWGG	5 cut(s)	202, 395, 918, 2255, 2320
Bst4CI	ACNGT	1 cut(s)	2468
Bst6I	CTCTTC	6 cut(s)	115, 122, 268, 899, 931, 2264
BstACI	GRCGYC	1 cut(s)	401
BstAPI	GCANNNNNTGC	1 cut(s)	577
BstBI	TTCGAA	1 cut(s)	929
BstC8I	GCNNGC	5 cut(s)	292, 462, 486, 1138, 2139
BstENI	CCTNNNNNAGG	2 cut(s)	944, 1246
BstF5I	GGATG	2 cut(s)	627, 1981
BstFNI	CGCG	1 cut(s)	338
BstH2I	RGCGCY	1 cut(s)	404
BstHHI	GCGC	6 cut(s)	108, 340, 403, 490, 526, 2137
BstKTI	GATC	6 cut(s)	1195, 1441, 1593, 1869, 1902, 1963
BstMAI	GTCTC	4 cut(s)	161, 1128, 1211, 2175
BstMBI	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
BstNI	CCWGG	5 cut(s)	202, 395, 918, 2255, 2320
BstNSI	RCATGY	3 cut(s)	828, 1453, 2155
BstSCI	CCNGG	7 cut(s)	200, 393, 435, 869, 916, 2253, 2318
BstSLI	GKGCMC	1 cut(s)	799
BstUI	CGCG	1 cut(s)	338
BstV2I	GAAGAC	1 cut(s)	1887
BstX2I	RGATCY	1 cut(s)	1590
BstYI	RGATCY	1 cut(s)	1590
BstZ17I	GTATAC	1 cut(s)	515
Bsu15I	ATCGAT	1 cut(s)	2116
Bsu36I	CCTNAGG	1 cut(s)	1215
BsuI	GTATCC	2 cut(s)	1033, 1655
BsuRI	GGCC	6 cut(s)	299, 497, 2080, 2088, 2163, 2382
BsuTUI	ATCGAT	1 cut(s)	2116
BtrI	CACGTC	1 cut(s)	1156
BtsCI	GGATG	2 cut(s)	627, 1981
BtsI	GCAGTG	1 cut(s)	449
BtsIMutI	CAGTG	7 cut(s)	449, 634, 799, 1404, 1505, 1921, 2473
Cac8I	GCNNGC	5 cut(s)	292, 462, 486, 1138, 2139
CciI	TCATGA	1 cut(s)	1386
CfoI	GCGC	6 cut(s)	108, 340, 403, 490, 526, 2137
Cfr10I	RCCGGY	3 cut(s)	243, 484, 497
Cfr13I	GGNCC	3 cut(s)	304, 1638, 2161
ClaI	ATCGAT	1 cut(s)	2116
CsiI	ACCWGGT	1 cut(s)	2318
Csp6I	GTAC	8 cut(s)	270, 501, 1173, 1453, 1527, 2007, 2149, 2155
CspCI	CAANNNNNGTGG	2 cut(s)	544, 579
CviQI	GTAC	8 cut(s)	270, 501, 1173, 1453, 1527, 2007, 2149, 2155
DinI	GGCGCC	1 cut(s)	402
DpnI	GATC	6 cut(s)	1194, 1440, 1592, 1868, 1901, 1962
DpnII	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
DraIII	CACNNNGTG	1 cut(s)	2132
EaeI	YGGCCR	2 cut(s)	2078, 2380
Eam1104I	CTCTTC	6 cut(s)	115, 122, 268, 899, 931, 2264
EarI	CTCTTC	6 cut(s)	115, 122, 268, 899, 931, 2264
EciI	GGCGGA	2 cut(s)	367, 463
Ecl136II	GAGCTC	3 cut(s)	17, 25, 433
Eco147I	AGGCCT	1 cut(s)	299
Eco24I	GRGCYC	4 cut(s)	19, 27, 435, 1273
Eco31I	GGTCTC	1 cut(s)	1211
Eco47I	GGWCC	2 cut(s)	304, 1638
Eco53kI	GAGCTC	3 cut(s)	17, 25, 433
Eco57I	CTGAAG	1 cut(s)	1829
Eco81I	CCTNAGG	1 cut(s)	1215
EcoICRI	GAGCTC	3 cut(s)	17, 25, 433
EcoNI	CCTNNNNNAGG	2 cut(s)	944, 1246
EcoRI	GAATTC	2 cut(s)	1394, 1921
EcoRII	CCWGG	5 cut(s)	200, 393, 916, 2253, 2318
EcoT38I	GRGCYC	4 cut(s)	19, 27, 435, 1273
EgeI	GGCGCC	1 cut(s)	402
EheI	GGCGCC	1 cut(s)	402
Esp3I	CGTCTC	1 cut(s)	2175
FaqI	GGGAC	3 cut(s)	1166, 1366, 2236
FauNDI	CATATG	1 cut(s)	1222
FbaI	TGATCA	1 cut(s)	1960
FblI	GTMKAC	1 cut(s)	514
Fnu4HI	GCNGC	1 cut(s)	1617
FokI	GGATG	2 cut(s)	634, 1968
FriOI	GRGCYC	4 cut(s)	19, 27, 435, 1273
Fsp4HI	GCNGC	1 cut(s)	1617
FspBI	CTAG	7 cut(s)	753, 854, 1274, 1578, 1838, 1926, 2351
FspI	TGCGCA	1 cut(s)	525
GlaI	GCGC	6 cut(s)	107, 339, 402, 489, 525, 2136
GluI	GCNGC	1 cut(s)	1617
GsaI	CCCAGC	1 cut(s)	1524
GsuI	CTGGAG	4 cut(s)	184, 446, 1275, 1695
HaeII	RGCGCY	1 cut(s)	404
HaeIII	GGCC	6 cut(s)	299, 497, 2080, 2088, 2163, 2382
HapII	CCGG	6 cut(s)	244, 437, 485, 498, 871, 1903
HhaI	GCGC	6 cut(s)	108, 340, 403, 490, 526, 2137
Hin1I	GRCGYC	1 cut(s)	401
Hin6I	GCGC	6 cut(s)	106, 338, 401, 488, 524, 2135
HinP1I	GCGC	6 cut(s)	106, 338, 401, 488, 524, 2135
HincII	GTYRAC	1 cut(s)	2422
HindII	GTYRAC	1 cut(s)	2422
HindIII	AAGCTT	1 cut(s)	407
HpaII	CCGG	6 cut(s)	244, 437, 485, 498, 871, 1903
HphI	GGTGA	4 cut(s)	21, 1570, 2118, 2278
Hpy166II	GTNNAC	3 cut(s)	515, 613, 2422
Hpy188I	TCNGA	7 cut(s)	304, 421, 1184, 1876, 2392, 2440, 2461
Hpy8I	GTNNAC	3 cut(s)	515, 613, 2422
HpyAV	CCTTC	4 cut(s)	70, 266, 1501, 1995
HpyCH4III	ACNGT	1 cut(s)	2468
HpyCH4IV	ACGT	6 cut(s)	285, 1155, 1372, 1731, 2184, 2418
HpySE526I	ACGT	6 cut(s)	285, 1155, 1372, 1731, 2184, 2418
Hsp92I	GRCGYC	1 cut(s)	401
HspAI	GCGC	6 cut(s)	106, 338, 401, 488, 524, 2135
KasI	GGCGCC	1 cut(s)	400
KpnI	GGTACC	1 cut(s)	504
KroI	GCCGGC	1 cut(s)	484
KroNI	GCCGGC	1 cut(s)	486
Ksp22I	TGATCA	1 cut(s)	1960
Kzo9I	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
LguI	GCTCTTC	1 cut(s)	115
LmnI	GCTCC	7 cut(s)	22, 146, 430, 438, 439, 1163, 2068
LweI	GCATC	5 cut(s)	541, 615, 1281, 1502, 1603
MabI	ACCWGGT	1 cut(s)	2318
MaeI	CTAG	7 cut(s)	753, 854, 1274, 1578, 1838, 1926, 2351
MaeII	ACGT	6 cut(s)	285, 1155, 1372, 1731, 2184, 2418
MaeIII	GTNAC	2 cut(s)	973, 2124
MalI	GATC	6 cut(s)	1194, 1440, 1592, 1868, 1901, 1962
MbiI	CCGCTC	2 cut(s)	141, 223
MboI	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
MflI	RGATCY	1 cut(s)	1590
MhlI	GDGCHC	5 cut(s)	19, 27, 435, 799, 1273
MlsI	TGGCCA	2 cut(s)	2080, 2382
MluNI	TGGCCA	2 cut(s)	2080, 2382
Mly113I	GGCGCC	1 cut(s)	401
MlyI	GAGTC	3 cut(s)	517, 978, 1388
Mox20I	TGGCCA	2 cut(s)	2080, 2382
MroNI	GCCGGC	1 cut(s)	484
MroXI	GAANNNNTTC	3 cut(s)	925, 1418, 2311
MscI	TGGCCA	2 cut(s)	2080, 2382
MseI	TTAA	9 cut(s)	735, 942, 1016, 1070, 1601, 1629, 1655, 1943, 2240
Msp20I	TGGCCA	2 cut(s)	2080, 2382
MspI	CCGG	6 cut(s)	244, 437, 485, 498, 871, 1903
MspR9I	CCNGG	7 cut(s)	202, 395, 437, 871, 918, 2255, 2320
MvaI	CCWGG	5 cut(s)	202, 395, 918, 2255, 2320
MvnI	CGCG	1 cut(s)	338
NaeI	GCCGGC	1 cut(s)	486
NarI	GGCGCC	1 cut(s)	401
NciI	CCSGG	2 cut(s)	437, 871
NdeI	CATATG	1 cut(s)	1222
NdeII	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
NgoMIV	GCCGGC	1 cut(s)	484
NlaIV	GGNNCC	6 cut(s)	248, 306, 402, 502, 922, 2162
NmuCI	GTSAC	1 cut(s)	2124
NsbI	TGCGCA	1 cut(s)	525
NspI	RCATGY	3 cut(s)	828, 1453, 2155
NspV	TTCGAA	1 cut(s)	929
PagI	TCATGA	1 cut(s)	1386
PceI	AGGCCT	1 cut(s)	299
PciI	ACATGT	2 cut(s)	1449, 2151
PciSI	GCTCTTC	1 cut(s)	115
PcsI	WCGNNNNNNNCGW	2 cut(s)	342, 461
PdiI	GCCGGC	1 cut(s)	486
PdmI	GAANNNNTTC	3 cut(s)	925, 1418, 2311
PfeI	GAWTC	7 cut(s)	583, 758, 847, 1484, 1550, 1834, 1871
PfoI	TCCNGGA	1 cut(s)	435
PkrI	GCNGC	1 cut(s)	1618
PleI	GAGTC	3 cut(s)	516, 978, 1387
PluTI	GGCGCC	1 cut(s)	404
PpsI	GAGTC	3 cut(s)	516, 978, 1387
PscI	ACATGT	2 cut(s)	1449, 2151
PshBI	ATTAAT	2 cut(s)	735, 1016
PsiI	TTATAA	1 cut(s)	1230
Psp124BI	GAGCTC	3 cut(s)	19, 27, 435
Psp6I	CCWGG	5 cut(s)	200, 393, 916, 2253, 2318
PspFI	CCCAGC	1 cut(s)	1520
PspGI	CCWGG	5 cut(s)	200, 393, 916, 2253, 2318
PspN4I	GGNNCC	6 cut(s)	248, 306, 402, 502, 922, 2162
PspPI	GGNCC	3 cut(s)	304, 1638, 2161
PsrI	GAACNNNNNNTAC	2 cut(s)	1519, 1551
PsuI	RGATCY	1 cut(s)	1590
RsaI	GTAC	8 cut(s)	271, 502, 1174, 1454, 1528, 2008, 2150, 2156
RsaNI	GTAC	8 cut(s)	270, 501, 1173, 1453, 1527, 2007, 2149, 2155
SacI	GAGCTC	3 cut(s)	19, 27, 435
SapI	GCTCTTC	1 cut(s)	115
SaqAI	TTAA	9 cut(s)	735, 942, 1016, 1070, 1601, 1629, 1655, 1943, 2240
SatI	GCNGC	1 cut(s)	1617
Sau3AI	GATC	6 cut(s)	1192, 1438, 1590, 1866, 1899, 1960
Sau96I	GGNCC	3 cut(s)	304, 1638, 2161
ScaI	AGTACT	2 cut(s)	271, 1174
SchI	GAGTC	3 cut(s)	517, 978, 1388
ScrFI	CCNGG	7 cut(s)	202, 395, 437, 871, 918, 2255, 2320
SduI	GDGCHC	5 cut(s)	19, 27, 435, 799, 1273
SexAI	ACCWGGT	1 cut(s)	2318
SfaNI	GCATC	5 cut(s)	541, 615, 1281, 1502, 1603
SfoI	GGCGCC	1 cut(s)	402
SfuI	TTCGAA	1 cut(s)	929
SinI	GGWCC	2 cut(s)	304, 1638
SmlI	CTYRAG	7 cut(s)	410, 505, 1129, 1359, 1505, 1768, 1774
SmoI	CTYRAG	7 cut(s)	410, 505, 1129, 1359, 1505, 1768, 1774
SseBI	AGGCCT	1 cut(s)	299
SsiI	CCGC	8 cut(s)	139, 221, 378, 448, 580, 674, 1616, 2066
SspDI	GGCGCC	1 cut(s)	400
SspI	AATATT	1 cut(s)	1756
SspMI	CTAG	7 cut(s)	753, 854, 1274, 1578, 1838, 1926, 2351
SstI	GAGCTC	3 cut(s)	19, 27, 435
StuI	AGGCCT	1 cut(s)	299
StyD4I	CCNGG	7 cut(s)	200, 393, 435, 869, 916, 2253, 2318
TaaI	ACNGT	1 cut(s)	2468
TaiI	ACGT	6 cut(s)	288, 1158, 1375, 1734, 2187, 2421
TaqI	TCGA	8 cut(s)	354, 455, 704, 929, 987, 1377, 1869, 2116
TaqII	GACCGA	1 cut(s)	1626
TatI	WGTACW	4 cut(s)	269, 1172, 1452, 2006
TauI	GCSGC	1 cut(s)	1619
TfiI	GAWTC	7 cut(s)	583, 758, 847, 1484, 1550, 1834, 1871
Tru1I	TTAA	9 cut(s)	735, 942, 1016, 1070, 1601, 1629, 1655, 1943, 2240
Tru9I	TTAA	9 cut(s)	735, 942, 1016, 1070, 1601, 1629, 1655, 1943, 2240
TscAI	CASTG	7 cut(s)	449, 641, 799, 1411, 1505, 1921, 2473
TseFI	GTSAC	1 cut(s)	2124
Tsp45I	GTSAC	1 cut(s)	2124
TspDTI	ATGAA	8 cut(s)	758, 953, 1387, 1411, 1460, 1893, 2163, 2463
TspGWI	ACGGA	1 cut(s)	265
TspRI	CASTG	7 cut(s)	449, 641, 799, 1411, 1505, 1921, 2473
VpaK11BI	GGWCC	2 cut(s)	304, 1638
VspI	ATTAAT	2 cut(s)	735, 1016
XagI	CCTNNNNNAGG	2 cut(s)	944, 1246
XapI	RAATTY	5 cut(s)	698, 990, 1394, 1417, 1921
XbaI	TCTAGA	1 cut(s)	2350
XceI	RCATGY	3 cut(s)	828, 1453, 2155
XcmI	CCANNNNNNNNNTGG	1 cut(s)	315
XmiI	GTMKAC	1 cut(s)	514
XmnI	GAANNNNTTC	3 cut(s)	925, 1418, 2311
XspI	CTAG	7 cut(s)	753, 854, 1274, 1578, 1838, 1926, 2351
ZrmI	AGTACT	2 cut(s)	271, 1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.