MD10G1102200.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015087
Physical Location & Seq
Reverse (-)
16677010 .. 16677789
780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1102200.v1.1.491

Sequence Viewer

Length: 417 bp
ATGCTGATGGCCATGGCAATTAGAGGCGGGGTTGCCAAATCGAAGATGTTTATGCTTAGTTCATATTGCAGTGAAGCTAAGCAAGCGCAAGGAGATCATCGACGCCATTTCAGCAGCAAGCTGAGGGGAGCTGCAGAGAACATATCAAGCAGAATGGTTCAAAGTGATAGTGAGGATACTGACATGATGGAAAGGGAGACTGGCAGTAATAGTTCATCGTCATGGAGGTGTAGCTGGGTGCCGCATCCAAAAACAGGAATATATGTTCCGAAAGGGCATGAAAAGGTGATGGATGATGTGCCTAAGGGAGCTGCTTCCTTTAACCAGACTTTCTGGCTCCGGAATGTCGACGGAGTACTTGAAAAACCCGATCCGGATACTCCTCCCGAGCATTACTATCACTACATGCATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_3

Protein Analysis

139

Amino Acids

15.65

Weight (kDa)

7.06

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3a PF03242 20 - 99 7e-08 Late embryogenesis abundant protein (LEA_3a subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19550
fragaria_vesca FvH4_2g04480
malus_domestica MD05G1096000.v1.1 MD10G1102200.v1.1
prunus_persica Prupe.8G140400_v2.0.a1
pyrus_communis pycom05g09470
rosa_chinensis RchiOBHm_Chr6g0253971
rosa_laevigata RLG00000014952
rosa_multiflora Rmu_sc0001716.1_g000012
rosa_roxburghii Rroxscaffold_7G00211360
rosa_rugosa Rorug05G0548700
rosa_samantha Rh6AG065000 Rh6BG058100 Rh6CG058100 Rh6DG055000
rosa_wichuraiana Rw6G005730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 348
AccIII TCCGGA 2 cut(s) 339, 373
AciI CCGC 2 cut(s) 27, 242
AclWI GGATC 1 cut(s) 365
AcoI YGGCCR 1 cut(s) 9
AcyI GRCGYC 1 cut(s) 103
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 2 cut(s) 161, 362
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
AluBI AGCT 5 cut(s) 77, 121, 131, 234, 311
AluI AGCT 5 cut(s) 77, 121, 131, 234, 311
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 365
Ama87I CYCGRG 1 cut(s) 386
Aor13HI TCCGGA 2 cut(s) 339, 373
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 3 cut(s) 114, 131, 311
AspLEI GCGC 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 298
AvaI CYCGRG 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 303
BalI TGGCCA 1 cut(s) 11
BanI GGYRCC 1 cut(s) 238
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 3 cut(s) 118, 126, 298
BccI CCATC 2 cut(s) 181, 283
BciVI GTATCC 2 cut(s) 169, 370
BcoDI GTCTC 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 132
BfuI GTATCC 2 cut(s) 169, 370
BisI GCNGC 4 cut(s) 115, 132, 242, 312
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 4 cut(s) 116, 133, 243, 313
BmcAI AGTACT 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 386
BmiI GGNNCC 2 cut(s) 240, 338
BmsI GCATC 1 cut(s) 253
Bpu10I CCTNAGC 1 cut(s) 122
Bpu1102I GCTNAGC 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 2 cut(s) 339, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 205
Bse21I CCTNAGG 1 cut(s) 303
BseAI TCCGGA 2 cut(s) 339, 373
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 2 cut(s) 244, 298
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 3 cut(s) 118, 126, 298
BseYI CCCAGC 1 cut(s) 234
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 2 cut(s) 340, 374
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 191
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 386
Bsp13I TCCGGA 2 cut(s) 339, 373
Bsp143I GATC 2 cut(s) 94, 370
Bsp1720I GCTNAGC 1 cut(s) 78
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 2 cut(s) 27, 242
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 114
BspEI TCCGGA 2 cut(s) 339, 373
BspLI GGNNCC 2 cut(s) 240, 338
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 1 cut(s) 365
BspT107I GGYRCC 1 cut(s) 238
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 2 cut(s) 94, 370
BssNI GRCGYC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 84, 119
BstDEI CTNAG 4 cut(s) 56, 78, 122, 303
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 244, 298
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 2 cut(s) 97, 373
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 2 cut(s) 94, 370
BstMWI GCNNNNNNNGC 2 cut(s) 83, 111
BstNSI RCATGY 1 cut(s) 409
BstSFI CTRYAG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 118, 126, 298
Bsu36I CCTNAGG 1 cut(s) 303
BsuI GTATCC 2 cut(s) 169, 370
BsuRI GGCC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 244, 298
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 2 cut(s) 84, 119
CfoI GCGC 1 cut(s) 88
CseI GACGC 1 cut(s) 111
Csp6I GTAC 1 cut(s) 356
CviAII CATG 5 cut(s) 13, 184, 222, 278, 406
CviJI RGCY 7 cut(s) 11, 77, 121, 131, 234, 311, 337
CviKI_1 RGCY 7 cut(s) 11, 77, 121, 131, 234, 311, 337
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 4 cut(s) 56, 78, 122, 303
DpnI GATC 2 cut(s) 96, 372
DpnII GATC 2 cut(s) 94, 370
EaeI YGGCCR 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 12
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 12
EcoT22I ATGCAT 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 5 cut(s) 16, 187, 225, 281, 409
FatI CATG 5 cut(s) 12, 183, 221, 277, 405
FauI CCCGC 1 cut(s) 20
FauNDI CATATG 1 cut(s) 411
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 4 cut(s) 115, 132, 242, 312
FokI GGATG 2 cut(s) 231, 305
Fsp4HI GCNGC 4 cut(s) 115, 132, 242, 312
GlaI GCGC 1 cut(s) 87
GluI GCNGC 4 cut(s) 115, 132, 242, 312
GsaI CCCAGC 1 cut(s) 238
HaeIII GGCC 1 cut(s) 11
HapII CCGG 2 cut(s) 340, 374
HgaI GACGC 1 cut(s) 111
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 103
Hin1II CATG 5 cut(s) 16, 187, 225, 281, 409
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HpaII CCGG 2 cut(s) 340, 374
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 3 cut(s) 340, 374, 386
Hpy8I GTNNAC 1 cut(s) 349
Hpy99I CGWCG 2 cut(s) 105, 353
HpyCH4V TGCA 3 cut(s) 69, 134, 409
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 111
HpyF3I CTNAG 4 cut(s) 56, 78, 122, 303
Hsp92I GRCGYC 1 cut(s) 103
Hsp92II CATG 5 cut(s) 16, 187, 225, 281, 409
HspAI GCGC 1 cut(s) 86
Kpn2I TCCGGA 2 cut(s) 339, 373
Kzo9I GATC 2 cut(s) 94, 370
LmnI GCTCC 3 cut(s) 128, 308, 342
LpnPI CCDG 7 cut(s) 186, 220, 240, 319, 338, 353, 387
Lsp1109I GCAGC 3 cut(s) 118, 126, 298
LweI GCATC 1 cut(s) 253
MalI GATC 2 cut(s) 96, 372
MboI GATC 2 cut(s) 94, 370
MboII GAAGA 1 cut(s) 55
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 1 cut(s) 18
MluNI TGGCCA 1 cut(s) 11
MnlI CCTC 5 cut(s) 17, 117, 166, 219, 393
Mox20I TGGCCA 1 cut(s) 11
Mph1103I ATGCAT 1 cut(s) 411
MroI TCCGGA 2 cut(s) 339, 373
MscI TGGCCA 1 cut(s) 11
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 3 cut(s) 220, 226, 410
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 2 cut(s) 340, 374
MwoI GCNNNNNNNGC 2 cut(s) 83, 111
NcoI CCATGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 411
NdeII GATC 2 cut(s) 94, 370
NlaIII CATG 5 cut(s) 16, 187, 225, 281, 409
NlaIV GGNNCC 2 cut(s) 240, 338
NsiI ATGCAT 1 cut(s) 411
NspI RCATGY 1 cut(s) 409
PkrI GCNGC 4 cut(s) 116, 133, 243, 313
PspFI CCCAGC 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 240, 338
PstI CTGCAG 1 cut(s) 136
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 3 cut(s) 220, 226, 410
SalI GTCGAC 1 cut(s) 347
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 4 cut(s) 115, 132, 242, 312
Sau3AI GATC 2 cut(s) 94, 370
ScaI AGTACT 1 cut(s) 357
SetI ASST 7 cut(s) 79, 123, 133, 230, 236, 288, 313
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 132
SmiMI CAYNNNNRTG 3 cut(s) 220, 226, 410
Sse9I AATT 1 cut(s) 18
SsiI CCGC 2 cut(s) 27, 242
StyI CCWWGG 1 cut(s) 12
TaqI TCGA 3 cut(s) 41, 100, 348
TasI AATT 1 cut(s) 18
TatI WGTACW 1 cut(s) 355
TauI GCSGC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 76
TseI GCWGC 3 cut(s) 114, 131, 311
TspDTI ATGAA 3 cut(s) 51, 204, 294
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 76
XceI RCATGY 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 348
ZrmI AGTACT 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.