MD10G1105600.v1.1
ERF Family

Interferon-related developmental regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008115
Physical Location & Seq
Reverse (-)
17260386 .. 17264864
4479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1105600.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGAGTTCTCACATGCTTTCGAACATGATGAGAAGAGGCAGAGCTGCTCCGCCTTCAGCCGCCCGAAAGCGCGGCGGCGAAAGAAAAACTGGTAGAAGACCTGGTGCAGTTGCAGCTGACGAGTCTACGGACAATGAACTGGTGATTCGGCCTCCAAAACTGAAGACACTTTCCTTTTTGTTAGAAGGGTTGTTTGAGAAAAGGGGATCCGACCGAGAGGAGGCATTGGCATCAATAATTGAGGGATTAAATCGTAAACTAGACCATGAGTTTCTGGAAAACAATTTTGCTACTTTTCTATTCAGAAGCTTGAATTGCCTAAAAAAGGGTTCTAACAGGGAGAAGCAGCTATCACTTCATGTCATTGGCTTGCTGGCCATAGTCATTTGTTGCGAGGATAAGTTGTCCGAAATATACAAAGAGTTGCTTCCTGTATTTTCCAAAGTTCTGAAATCTGGACCCACGACCTTAAAGATATTGGATTGTTTGGCTATTGTGGCTTTCTTTGGTGCAACTAACTCGGAGGAGACAGAAAGTGCAATGCAAATTATTTGGAAGTTCATTAATCCTGAGTCTGCCTCAGATGTCAACACCCAAAACCATTCGGCAGAGATCCTAGTTGCTGCAGTATATGCTTGGTTGTTTCTTCTTACAAGTATGGAGGGATGGAGACTCAGCCACAACTATTGGAATGGGGCCATTTCTTACTTCTCAAACCTATTAGAGCACGATAATAAATTAGTCCGCATAGCAGCCAGTGAAGCGCTGGCTTTGATATTTGAAACTGGTAGTCTGGAGAAGTTCTGGAGTGAAGCAAAGGATCCCAGCCTCACAAAATATTCACACATGCAACTATTACTGAAAGAGAATGTCTTAAAGAAGTTAAACTGTCTTTATTCGGACATAAGAAATGAGAATGTTGCAAACAAAGTCCGTGAGGTTGTGAAATATTTTGAGAGTTCTCGTCCGTCAGAATTTGTTCTAACAGTCAATGGAAAAGGACTAAAGTTATCATCATGGTATCAAATTATTCAGTTACAGTTTTTGAAGAGTTTTTTAGCTGGCGGATTTGAGAACCACATGAAGGAAAATGAGAAACTACAGAATTTGTTCGAGTTTAATCCACACAGAAACAAAAATTTAGGCCCAGAATTGTATGAATCTACCACTGATAAGATTACTGTCTGTTTCTTTGTACCTGAAGAAAGAGATCCAGATAAACTGACAAAGGAAGATAGAAAGAAAGAGAGGGTTTGGAGGAATGCACTATTAGACAAGGCAAGAACACAACTAATGAGAAAACATCGGCTAATGTCACAGGAAATGAATAGCTGCTGCTACGATTTTGAACAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IFRD IFRD_C

Protein Analysis

453

Amino Acids

51.9

Weight (kDa)

8.71

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IFRD PF05004 55 - 300 1.8e-50 Interferon-related developmental regulator (IFRD)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 125
AccII CGCG 1 cut(s) 72
AciI CCGC 6 cut(s) 50, 60, 72, 75, 745, 1065
AclWI GGATC 6 cut(s) 201, 214, 607, 815, 828, 1205
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 3 cut(s) 974, 1105, 1138
AcuI CTGAAG 3 cut(s) 39, 182, 1221
AfaI GTAC 1 cut(s) 1197
AfeI AGCGCT 1 cut(s) 765
AfiI CCNNNNNNNGG 3 cut(s) 220, 325, 1084
AgsI TTSAA 4 cut(s) 313, 782, 1048, 1349
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 6 cut(s) 44, 116, 309, 349, 1061, 1332
AluI AGCT 6 cut(s) 44, 116, 309, 349, 1061, 1332
Alw21I GWGCWC 1 cut(s) 729
Alw26I GTCTC 2 cut(s) 521, 664
AlwI GGATC 6 cut(s) 201, 214, 607, 815, 828, 1205
Aor51HI AGCGCT 1 cut(s) 765
AoxI GGCC 4 cut(s) 149, 375, 696, 1144
ApeKI GCWGC 7 cut(s) 44, 113, 346, 623, 752, 1332, 1335
ApoI RAATTY 3 cut(s) 974, 1105, 1138
AseI ATTAAT 1 cut(s) 564
Asp700I GAANNNNTTC 2 cut(s) 978, 1109
AspLEI GCGC 2 cut(s) 72, 766
AspS9I GGNCC 3 cut(s) 458, 696, 1145
AsuHPI GGTGA 1 cut(s) 154
AsuII TTCGAA 1 cut(s) 20
AvaII GGWCC 1 cut(s) 458
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 2 cut(s) 206, 820
BbsI GAAGAC 2 cut(s) 103, 170
Bbv12I GWGCWC 1 cut(s) 729
BbvI GCAGC 7 cut(s) 31, 125, 358, 610, 764, 1319, 1322
BccI CCATC 1 cut(s) 660
BcgI CGANNNNNNTGC 2 cut(s) 501, 535
BciT130I CCWGG 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 521, 664
BfaI CTAG 2 cut(s) 260, 617
BfmI CTRYAG 2 cut(s) 624, 1100
BfoI RGCGCY 1 cut(s) 767
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 3 cut(s) 458, 696, 1145
BmiI GGNNCC 4 cut(s) 208, 460, 697, 822
BmrFI CCNGG 1 cut(s) 102
BmsI GCATC 1 cut(s) 239
BpiI GAAGAC 2 cut(s) 103, 170
BplI GAGNNNNNCTC 2 cut(s) 563, 595
BpmI CTGGAG 2 cut(s) 815, 826
Bpu14I TTCGAA 1 cut(s) 20
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 325, 1084
Bse1I ACTGG 4 cut(s) 94, 144, 756, 790
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 3 cut(s) 220, 325, 1084
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 3 cut(s) 561, 594, 688
BseNI ACTGG 4 cut(s) 94, 144, 756, 790
BseRI GAGGAG 2 cut(s) 233, 539
BseXI GCAGC 7 cut(s) 31, 125, 358, 610, 764, 1319, 1322
BseYI CCCAGC 1 cut(s) 824
BsgI GTGCAG 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 72
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 151, 377, 698, 1146
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 729
BslI CCNNNNNNNGG 3 cut(s) 220, 325, 1084
BsmAI GTCTC 2 cut(s) 521, 664
BsmI GAATGC 1 cut(s) 1267
BsnI GGCC 4 cut(s) 151, 377, 698, 1146
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 729
Bsp143I GATC 4 cut(s) 206, 612, 820, 1210
BspACI CCGC 6 cut(s) 50, 60, 72, 75, 745, 1065
BspANI GGCC 4 cut(s) 151, 377, 698, 1146
BspCNI CTCAG 3 cut(s) 562, 593, 687
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 4 cut(s) 208, 460, 697, 822
BspMAI CTGCAG 1 cut(s) 628
BspPI GGATC 6 cut(s) 201, 214, 607, 815, 828, 1205
BspT104I TTCGAA 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 4 cut(s) 94, 144, 756, 790
BssMI GATC 4 cut(s) 206, 612, 820, 1210
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 5 cut(s) 890, 988, 1041, 1183, 1353
Bst6I CTCTTC 2 cut(s) 28, 1043
BstAPI GCANNNNNTGC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 4 cut(s) 371, 375, 768, 1063
BstDEI CTNAG 3 cut(s) 570, 580, 674
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 671
BstFNI CGCG 1 cut(s) 72
BstH2I RGCGCY 1 cut(s) 767
BstHHI GCGC 2 cut(s) 72, 766
BstKTI GATC 4 cut(s) 209, 615, 823, 1213
BstMAI GTCTC 2 cut(s) 521, 664
BstMBI GATC 4 cut(s) 206, 612, 820, 1210
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 5 cut(s) 113, 315, 497, 632, 761
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 2 cut(s) 16, 850
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 2 cut(s) 624, 1100
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 7 cut(s) 31, 125, 358, 610, 764, 1319, 1322
BstV2I GAAGAC 2 cut(s) 103, 170
BstX2I RGATCY 4 cut(s) 206, 612, 820, 1210
BstYI RGATCY 4 cut(s) 206, 612, 820, 1210
BsuRI GGCC 4 cut(s) 151, 377, 698, 1146
BtsCI GGATG 1 cut(s) 671
BtsIMutI CAGTG 2 cut(s) 763, 1167
Cac8I GCNNGC 4 cut(s) 371, 375, 768, 1063
CfoI GCGC 2 cut(s) 72, 766
Cfr13I GGNCC 3 cut(s) 458, 696, 1145
CsiI ACCWGGT 1 cut(s) 100
Csp6I GTAC 1 cut(s) 1196
CspCI CAANNNNNGTGG 2 cut(s) 668, 703
CviAII CATG 7 cut(s) 13, 25, 266, 359, 847, 1017, 1081
CviQI GTAC 1 cut(s) 1196
DdeI CTNAG 3 cut(s) 570, 580, 674
DpnI GATC 4 cut(s) 208, 614, 822, 1212
DpnII GATC 4 cut(s) 206, 612, 820, 1210
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 2 cut(s) 28, 1043
EarI CTCTTC 2 cut(s) 28, 1043
EciI GGCGGA 2 cut(s) 39, 1080
Eco47I GGWCC 1 cut(s) 458
Eco47III AGCGCT 1 cut(s) 765
Eco57I CTGAAG 3 cut(s) 39, 182, 1221
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 100
FaeI CATG 7 cut(s) 16, 28, 269, 362, 850, 1020, 1084
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 7 cut(s) 12, 24, 265, 358, 846, 1016, 1080
FblI GTMKAC 1 cut(s) 125
FokI GGATG 1 cut(s) 678
FspBI CTAG 2 cut(s) 260, 617
GlaI GCGC 2 cut(s) 71, 765
GsaI CCCAGC 1 cut(s) 828
GsuI CTGGAG 2 cut(s) 815, 826
HaeII RGCGCY 1 cut(s) 767
HaeIII GGCC 4 cut(s) 151, 377, 698, 1146
HhaI GCGC 2 cut(s) 72, 766
Hin1II CATG 7 cut(s) 16, 28, 269, 362, 850, 1020, 1084
Hin6I GCGC 2 cut(s) 70, 764
HinP1I GCGC 2 cut(s) 70, 764
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 307
HinfI GANTC 5 cut(s) 122, 145, 572, 672, 1160
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 3 cut(s) 126, 257, 589
Hpy188I TCNGA 8 cut(s) 211, 305, 409, 450, 523, 583, 901, 973
Hpy188III TCNNGA 6 cut(s) 275, 456, 569, 794, 805, 1214
Hpy8I GTNNAC 3 cut(s) 126, 257, 589
HpyAV CCTTC 3 cut(s) 63, 179, 1078
HpyCH4III ACNGT 5 cut(s) 890, 988, 1041, 1183, 1353
HpyCH4V TGCA 9 cut(s) 107, 113, 512, 539, 544, 626, 850, 923, 1265
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 315, 497, 632, 761
HpyF3I CTNAG 3 cut(s) 570, 580, 674
Hsp92II CATG 7 cut(s) 16, 28, 269, 362, 850, 1020, 1084
HspAI GCGC 2 cut(s) 70, 764
Kzo9I GATC 4 cut(s) 206, 612, 820, 1210
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 7 cut(s) 31, 125, 358, 610, 764, 1319, 1322
LweI GCATC 1 cut(s) 239
MabI ACCWGGT 1 cut(s) 100
MaeI CTAG 2 cut(s) 260, 617
MaeIII GTNAC 2 cut(s) 1035, 1314
MalI GATC 4 cut(s) 208, 614, 822, 1212
MboI GATC 4 cut(s) 206, 612, 820, 1210
MboII GAAGA 7 cut(s) 45, 108, 175, 638, 1060, 1214, 1244
MflI RGATCY 4 cut(s) 206, 612, 820, 1210
MhlI GDGCHC 1 cut(s) 729
MlsI TGGCCA 1 cut(s) 377
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 3 cut(s) 131, 581, 666
MmeI TCCRAC 1 cut(s) 234
Mox20I TGGCCA 1 cut(s) 377
MroXI GAANNNNTTC 2 cut(s) 978, 1109
MscI TGGCCA 1 cut(s) 377
MseI TTAA 7 cut(s) 248, 470, 564, 875, 884, 1119, 1357
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 1267
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 5 cut(s) 113, 315, 497, 632, 761
NdeII GATC 4 cut(s) 206, 612, 820, 1210
NlaIII CATG 7 cut(s) 16, 28, 269, 362, 850, 1020, 1084
NlaIV GGNNCC 4 cut(s) 208, 460, 697, 822
NmuCI GTSAC 1 cut(s) 1314
NspI RCATGY 2 cut(s) 16, 850
NspV TTCGAA 1 cut(s) 20
PctI GAATGC 1 cut(s) 1267
PdmI GAANNNNTTC 2 cut(s) 978, 1109
PfeI GAWTC 2 cut(s) 145, 1160
PleI GAGTC 3 cut(s) 130, 580, 666
PpsI GAGTC 3 cut(s) 130, 580, 666
PshBI ATTAAT 1 cut(s) 564
Psp6I CCWGG 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 4 cut(s) 208, 460, 697, 822
PspPI GGNCC 3 cut(s) 458, 696, 1145
PstI CTGCAG 1 cut(s) 628
PsuI RGATCY 4 cut(s) 206, 612, 820, 1210
PvuII CAGCTG 1 cut(s) 116
RsaI GTAC 1 cut(s) 1197
RsaNI GTAC 1 cut(s) 1196
SaqAI TTAA 7 cut(s) 248, 470, 564, 875, 884, 1119, 1357
Sau3AI GATC 4 cut(s) 206, 612, 820, 1210
Sau96I GGNCC 3 cut(s) 458, 696, 1145
SchI GAGTC 3 cut(s) 131, 581, 666
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 1 cut(s) 729
SexAI ACCWGGT 1 cut(s) 100
SfaNI GCATC 1 cut(s) 239
SfcI CTRYAG 2 cut(s) 624, 1100
SfuI TTCGAA 1 cut(s) 20
SinI GGWCC 1 cut(s) 458
SsiI CCGC 6 cut(s) 50, 60, 72, 75, 745, 1065
SspI AATATT 2 cut(s) 839, 950
SspMI CTAG 2 cut(s) 260, 617
StyD4I CCNGG 1 cut(s) 100
TaaI ACNGT 5 cut(s) 890, 988, 1041, 1183, 1353
TaqI TCGA 2 cut(s) 20, 1113
TaqII GACCGA 1 cut(s) 228
TauI GCSGC 3 cut(s) 62, 75, 78
TfiI GAWTC 2 cut(s) 145, 1160
Tru1I TTAA 7 cut(s) 248, 470, 564, 875, 884, 1119, 1357
Tru9I TTAA 7 cut(s) 248, 470, 564, 875, 884, 1119, 1357
TscAI CASTG 2 cut(s) 763, 1174
TseFI GTSAC 1 cut(s) 1314
TseI GCWGC 7 cut(s) 44, 113, 346, 623, 752, 1332, 1335
Tsp45I GTSAC 1 cut(s) 1314
TspDTI ATGAA 6 cut(s) 150, 347, 550, 1097, 1173, 1340
TspGWI ACGGA 3 cut(s) 143, 923, 957
TspRI CASTG 2 cut(s) 763, 1174
VpaK11BI GGWCC 1 cut(s) 458
VspI ATTAAT 1 cut(s) 564
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 3 cut(s) 974, 1105, 1138
XceI RCATGY 2 cut(s) 16, 850
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
XmiI GTMKAC 1 cut(s) 125
XmnI GAANNNNTTC 2 cut(s) 978, 1109
XspI CTAG 2 cut(s) 260, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.