Rh6DG053000
ERF Family

Interferon-related developmental regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008115
Physical Location & Seq
Reverse (-)
5554796 .. 5558666
3871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG053000.1

Sequence Viewer

Length: 1386 bp
ATGAGTAGTCGTCGCGGGTTGTCGAACATGAGAACAAGAGGTAGTCTTCATCCTCCAAATGCCATGAGGAAAAAAGGCAAACAAAAAGCTGGAGGAAGAACTGTTGCTGATGAGTATGAGGATGAGTTACCGGAAAAACTGACTCAGGTTCCTCGTCCCTGCCCCTCGGGTCCAATTTCTACTTACTTAGTAGAAGGGTTATATGAGAAAAGGGGAGATAAGCGAGAGGAGACACTAATCTTTATGATTGAAGGCTTAAATAGAAACCTAAGCAAAAACTTGCTGGAAGAGAAGTTTGCTACCTTTCTATACAGATGCTTGAACTCTGTGAAGAAAGGTTCTACCAAGGAGGTGAATAATTCATTTCATGTGATAGGCTTGCTGGCAACAATAGTTGGTTGTGAAGATAAACTCTCTGAAATATATAGAGAGTTGCTTCCTGTACTTTCCGGAGCTCTGAAATCTGGGTCCACAACAATAAAGATATTGGATTGCTTGGCTATTGTTGCTTTCTTTGGTGCAACCAATCCAGATGAAACAGAGAATGCCATGCATATTATATGGGAGTACCTCAATCCCGAGTTGGGTTATGATGTCAACACAAAAAATAATTCAGCAGAGTTACTAGTTGCTGCAGTATATGCTTGGTTGTTTCTTCTTTCAAGCATGGAGGGATTGAGACTCAGCTACACCTACTGGAAAGGGGCCATTTCATACTTCTCAAATCTACTAGATCACGACAACAAATTAGTATGTGGAGCAGCTAGTGAAGCATTAGCTTTAATTTTTGAAACTGGCTCTCTTGAAAAGTTCTGGAATGAAGAAAATGGTTCAAGCCCCAGTTATTCACAAATGCAACAATCACTTGGAGCCCATCTTTTAAAGAAAGTTAAATGTGCTTACAAAGATCTACGAACTGAGAATAATGCAGACAAAATCCGCAATGTTGTAAGATATTTTGAGAGTTCTCATGTCCTGGCAGAATCTTTAGTGGTCAAAGGGAAAGTACTAACGTTGTCATCATGGCATCAAATAATTCAGCTGCAGTTTTTGAAGAGGTTTCTAGCTGATGGATTTAAAAAACATATGGAGGAAAATGAGAAGCTTCAGAATTTGTTCGAGTTCGATCCTCGTAAAAGCAAGAATTTAGGTCCAGAGTTGTATGTAGCTACTACTGATAAGATCTTTGTGCGCTTCTTTATGCCTGAAGAAAGAGATCAATACATGCTAACGAAGGAACAAAGAAAGAAAGAAAGGCTTAGGAGGAGCTCTATTGTAGACAAAGCCCAGACACAATTGATGACAAAACATCGTTTGTTGTCGAAGGAACTAAAACGCTGCCACTATGATTTTGAAAGTGAACAACAATCAAGATCACCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IFRD IFRD_C

Protein Analysis

461

Amino Acids

52.8

Weight (kDa)

8.88

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IFRD PF05004 64 - 299 1.1e-42 Interferon-related developmental regulator (IFRD)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1278
AccII CGCG 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 449
AciI CCGC 2 cut(s) 15, 940
AclI AACGTT 1 cut(s) 1013
AclWI GGATC 1 cut(s) 1121
AcsI RAATTY 2 cut(s) 1111, 1144
AcuI CTGAAG 2 cut(s) 1091, 1227
AfaI GTAC 3 cut(s) 444, 569, 1008
AfiI CCNNNNNNNGG 1 cut(s) 584
AgsI TTSAA 8 cut(s) 251, 322, 663, 791, 806, 834, 1054, 1355
AhlI ACTAGT 1 cut(s) 625
AjnI CCWGG 1 cut(s) 975
Alw21I GWGCWC 2 cut(s) 457, 1271
Alw26I GTCTC 2 cut(s) 224, 673
AlwI GGATC 1 cut(s) 1121
Ama87I CYCGRG 2 cut(s) 166, 578
Aor13HI TCCGGA 1 cut(s) 449
AoxI GGCC 1 cut(s) 705
ApeKI GCWGC 4 cut(s) 632, 761, 1042, 1338
ApoI RAATTY 2 cut(s) 1111, 1144
Asp700I GAANNNNTTC 1 cut(s) 1115
AspLEI GCGC 1 cut(s) 1194
AspS9I GGNCC 4 cut(s) 170, 468, 705, 1151
AsuHPI GGTGA 2 cut(s) 364, 1368
AvaI CYCGRG 2 cut(s) 166, 578
AvaII GGWCC 3 cut(s) 170, 468, 1151
BanII GRGCYC 3 cut(s) 457, 874, 1271
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 457, 1271
BbvI GCAGC 4 cut(s) 619, 773, 1029, 1325
BccI CCATC 2 cut(s) 882, 1064
BciT130I CCWGG 1 cut(s) 977
BcoDI GTCTC 2 cut(s) 224, 673
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 5 cut(s) 626, 731, 765, 1064, 1384
BfmI CTRYAG 2 cut(s) 633, 1043
BglII AGATCT 2 cut(s) 907, 1182
BisI GCNGC 4 cut(s) 633, 762, 1043, 1339
BlsI GCNGC 4 cut(s) 634, 763, 1044, 1340
BmcAI AGTACT 1 cut(s) 1008
Bme1390I CCNGG 1 cut(s) 977
Bme18I GGWCC 3 cut(s) 170, 468, 1151
BmeT110I CYCGRG 2 cut(s) 166, 578
BmgT120I GGNCC 4 cut(s) 170, 468, 705, 1151
BmiI GGNNCC 5 cut(s) 150, 171, 469, 706, 871
BmrFI CCNGG 1 cut(s) 977
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 2 cut(s) 305, 1036
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 111
Bpu10I CCTNAGC 2 cut(s) 269, 1259
BsaJI CCNNGG 2 cut(s) 165, 345
BsaWI WCCGGW 2 cut(s) 130, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 3 cut(s) 701, 799, 840
Bse3DI GCAATG 1 cut(s) 949
BseAI TCCGGA 1 cut(s) 449
BseBI CCWGG 1 cut(s) 977
BseDI CCNNGG 2 cut(s) 165, 345
BseGI GGATG 2 cut(s) 49, 127
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMI GCAATG 1 cut(s) 949
BseMII CTCAG 3 cut(s) 158, 697, 909
BseNI ACTGG 3 cut(s) 701, 799, 840
BseRI GAGGAG 2 cut(s) 242, 1279
BseXI GCAGC 4 cut(s) 619, 773, 1029, 1325
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 1 cut(s) 707
BsiHKAI GWGCWC 2 cut(s) 457, 1271
BsiHKCI CYCGRG 2 cut(s) 166, 578
BsiSI CCGG 2 cut(s) 131, 450
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 2 cut(s) 224, 673
BsmFI GGGAC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 1 cut(s) 707
BsoBI CYCGRG 2 cut(s) 166, 578
Bsp1286I GDGCHC 3 cut(s) 457, 874, 1271
Bsp13I TCCGGA 1 cut(s) 449
Bsp143I GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
BspACI CCGC 2 cut(s) 15, 940
BspANI GGCC 1 cut(s) 707
BspCNI CTCAG 3 cut(s) 157, 696, 910
BspEI TCCGGA 1 cut(s) 449
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 5 cut(s) 150, 171, 469, 706, 871
BspMAI CTGCAG 2 cut(s) 637, 1047
BspPI GGATC 1 cut(s) 1121
BsrDI GCAATG 1 cut(s) 949
BsrI ACTGG 3 cut(s) 701, 799, 840
BssECI CCNNGG 2 cut(s) 165, 345
BssMI GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 977
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 2 cut(s) 282, 1049
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 2 cut(s) 380, 384
BstDEI CTNAG 6 cut(s) 144, 187, 269, 683, 918, 1259
BstF5I GGATG 2 cut(s) 49, 127
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 1194
BstKTI GATC 6 cut(s) 736, 910, 1129, 1185, 1219, 1376
BstMAI GTCTC 2 cut(s) 224, 673
BstMBI GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
BstMWI GCNNNNNNNGC 3 cut(s) 506, 641, 770
BstNI CCWGG 1 cut(s) 977
BstNSI RCATGY 1 cut(s) 1228
BstSCI CCNGG 1 cut(s) 975
BstSFI CTRYAG 2 cut(s) 633, 1043
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 4 cut(s) 619, 773, 1029, 1325
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 2 cut(s) 907, 1182
BstYI RGATCY 2 cut(s) 907, 1182
BsuRI GGCC 1 cut(s) 707
BtsCI GGATG 2 cut(s) 49, 127
Cac8I GCNNGC 2 cut(s) 380, 384
CfoI GCGC 1 cut(s) 1194
Cfr13I GGNCC 4 cut(s) 170, 468, 705, 1151
Csp6I GTAC 3 cut(s) 443, 568, 1007
CviAII CATG 8 cut(s) 28, 64, 368, 550, 667, 971, 1023, 1225
CviQI GTAC 3 cut(s) 443, 568, 1007
DdeI CTNAG 6 cut(s) 144, 187, 269, 683, 918, 1259
DpnI GATC 6 cut(s) 735, 909, 1128, 1184, 1218, 1375
DpnII GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
DraI TTTAAA 2 cut(s) 882, 1078
Eam1104I CTCTTC 2 cut(s) 282, 1049
EarI CTCTTC 2 cut(s) 282, 1049
Ecl136II GAGCTC 2 cut(s) 455, 1269
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 3 cut(s) 457, 874, 1271
Eco47I GGWCC 3 cut(s) 170, 468, 1151
Eco53kI GAGCTC 2 cut(s) 455, 1269
Eco57I CTGAAG 2 cut(s) 1091, 1227
Eco88I CYCGRG 2 cut(s) 166, 578
EcoICRI GAGCTC 2 cut(s) 455, 1269
EcoRII CCWGG 1 cut(s) 975
EcoT14I CCWWGG 1 cut(s) 345
EcoT22I ATGCAT 1 cut(s) 555
EcoT38I GRGCYC 3 cut(s) 457, 874, 1271
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
FalI AAGNNNNNCTT 2 cut(s) 1242, 1274
FaqI GGGAC 1 cut(s) 141
FatI CATG 8 cut(s) 27, 63, 367, 549, 666, 970, 1022, 1224
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 1 cut(s) 1086
FblI GTMKAC 1 cut(s) 1278
Fnu4HI GCNGC 4 cut(s) 633, 762, 1043, 1339
FokI GGATG 2 cut(s) 36, 134
FriOI GRGCYC 3 cut(s) 457, 874, 1271
Fsp4HI GCNGC 4 cut(s) 633, 762, 1043, 1339
FspBI CTAG 5 cut(s) 626, 731, 765, 1064, 1384
GlaI GCGC 1 cut(s) 1193
GluI GCNGC 4 cut(s) 633, 762, 1043, 1339
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 1 cut(s) 707
HapII CCGG 2 cut(s) 131, 450
HhaI GCGC 1 cut(s) 1194
Hin1II CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
Hin6I GCGC 1 cut(s) 1192
HinP1I GCGC 1 cut(s) 1192
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 3 cut(s) 142, 681, 983
HpaII CCGG 2 cut(s) 131, 450
HphI GGTGA 2 cut(s) 364, 1368
Hpy166II GTNNAC 4 cut(s) 471, 598, 1279, 1361
Hpy188I TCNGA 3 cut(s) 418, 459, 1110
Hpy188III TCNNGA 8 cut(s) 450, 530, 578, 737, 803, 814, 1154, 1371
Hpy8I GTNNAC 4 cut(s) 471, 598, 1279, 1361
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 4 cut(s) 188, 245, 1228, 1318
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 1013
HpyCH4V TGCA 6 cut(s) 521, 553, 635, 856, 929, 1045
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 641, 770
HpyF3I CTNAG 6 cut(s) 144, 187, 269, 683, 918, 1259
HpySE526I ACGT 1 cut(s) 1013
Hsp92II CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
HspAI GCGC 1 cut(s) 1192
Kpn2I TCCGGA 1 cut(s) 449
Kzo9I GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
LmnI GCTCC 4 cut(s) 452, 758, 869, 1266
Lsp1109I GCAGC 4 cut(s) 619, 773, 1029, 1325
LweI GCATC 2 cut(s) 305, 1036
MaeI CTAG 5 cut(s) 626, 731, 765, 1064, 1384
MaeII ACGT 1 cut(s) 1013
MaeIII GTNAC 2 cut(s) 126, 621
MalI GATC 6 cut(s) 735, 909, 1128, 1184, 1218, 1375
MboI GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
MboII GAAGA 9 cut(s) 38, 108, 299, 343, 416, 647, 833, 1066, 1220
MfeI CAATTG 1 cut(s) 1295
MflI RGATCY 2 cut(s) 907, 1182
MhlI GDGCHC 3 cut(s) 457, 874, 1271
MluCI AATT 9 cut(s) 174, 358, 610, 746, 783, 1035, 1111, 1144, 1295
MlyI GAGTC 2 cut(s) 136, 675
Mph1103I ATGCAT 1 cut(s) 555
MroI TCCGGA 1 cut(s) 449
MroXI GAANNNNTTC 1 cut(s) 1115
MseI TTAA 5 cut(s) 257, 782, 881, 891, 1077
MspA1I CMGCKG 1 cut(s) 1042
MspI CCGG 2 cut(s) 131, 450
MspR9I CCNGG 1 cut(s) 977
MunI CAATTG 1 cut(s) 1295
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 1 cut(s) 977
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 506, 641, 770
NdeI CATATG 1 cut(s) 1086
NdeII GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
NlaIII CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
NlaIV GGNNCC 5 cut(s) 150, 171, 469, 706, 871
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 1 cut(s) 1228
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 1115
PfeI GAWTC 1 cut(s) 983
PkrI GCNGC 4 cut(s) 634, 763, 1044, 1340
PleI GAGTC 2 cut(s) 136, 675
PpsI GAGTC 2 cut(s) 136, 675
Psp124BI GAGCTC 2 cut(s) 457, 1271
Psp1406I AACGTT 1 cut(s) 1013
Psp6I CCWGG 1 cut(s) 975
PspGI CCWGG 1 cut(s) 975
PspN4I GGNNCC 5 cut(s) 150, 171, 469, 706, 871
PspPI GGNCC 4 cut(s) 170, 468, 705, 1151
PstI CTGCAG 2 cut(s) 637, 1047
PsuI RGATCY 2 cut(s) 907, 1182
PvuII CAGCTG 1 cut(s) 1042
RsaI GTAC 3 cut(s) 444, 569, 1008
RsaNI GTAC 3 cut(s) 443, 568, 1007
SacI GAGCTC 2 cut(s) 457, 1271
SaqAI TTAA 5 cut(s) 257, 782, 881, 891, 1077
SatI GCNGC 4 cut(s) 633, 762, 1043, 1339
Sau3AI GATC 6 cut(s) 733, 907, 1126, 1182, 1216, 1373
Sau96I GGNCC 4 cut(s) 170, 468, 705, 1151
ScaI AGTACT 1 cut(s) 1008
SchI GAGTC 2 cut(s) 136, 675
ScrFI CCNGG 1 cut(s) 977
SduI GDGCHC 3 cut(s) 457, 874, 1271
SfaNI GCATC 2 cut(s) 305, 1036
SfcI CTRYAG 2 cut(s) 633, 1043
SinI GGWCC 3 cut(s) 170, 468, 1151
SpeI ACTAGT 1 cut(s) 625
Sse9I AATT 9 cut(s) 174, 358, 610, 746, 783, 1035, 1111, 1144, 1295
SsiI CCGC 2 cut(s) 15, 940
SspMI CTAG 5 cut(s) 626, 731, 765, 1064, 1384
SstI GAGCTC 2 cut(s) 457, 1271
StyD4I CCNGG 1 cut(s) 975
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 1 cut(s) 1016
TaqI TCGA 4 cut(s) 23, 1119, 1125, 1322
TasI AATT 9 cut(s) 174, 358, 610, 746, 783, 1035, 1111, 1144, 1295
TatI WGTACW 2 cut(s) 442, 1006
TfiI GAWTC 1 cut(s) 983
Tru1I TTAA 5 cut(s) 257, 782, 881, 891, 1077
Tru9I TTAA 5 cut(s) 257, 782, 881, 891, 1077
TseI GCWGC 4 cut(s) 632, 761, 1042, 1338
TspDTI ATGAA 6 cut(s) 38, 351, 356, 549, 702, 834
VpaK11BI GGWCC 3 cut(s) 170, 468, 1151
XapI RAATTY 2 cut(s) 1111, 1144
XceI RCATGY 1 cut(s) 1228
XmiI GTMKAC 1 cut(s) 1278
XmnI GAANNNNTTC 1 cut(s) 1115
XspI CTAG 5 cut(s) 626, 731, 765, 1064, 1384
ZrmI AGTACT 1 cut(s) 1008
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.