MD10G1148800.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008060
Physical Location & Seq
Reverse (-)
23617942 .. 23624828
6887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1148800.v1.1.491

Sequence Viewer

Length: 2598 bp
ATGCATCTTGTGTACGATGTTGTCTTTGATCATCTTTCTAAGTATCTTGCAGTCGTGTGGCCTGATATCCCTTACTTCTCTATATACAAAGTTAGTGACTGGTCAATTGTTGATTCAGGCAGTGCAAGGCTTCTGGCTTGGGATACATGTCGTGACAGATTTGCTATACTAGAGTCTACATTACCACCTCGAATTCCTGTAATTCATAAGGGAGGATCATCGAGAAAAGCAAAAGAAGCTGCAGCAGCTGCGGCAGCTGCCTCTGCTGCTTCTGCAGCTAACGTACAAGTTCGCATCTTGCTGGATGATGGCACATCAAATATTTTAATGAGATCTATAGGTGGTCGCAGTGACCCGGTCATTGGCTTGCATGGAGGAGCACTTCTTGGTGTTGCATATCGAATATCTCGGAGAATTAGCCCTATCTCCGCAACAGCTATTTCAACAATTCAATCCATGCCCTTGTCTGGATTTGGAAGTGCTGGGCATTCTTCTTTTTCCACTTTCGATGATGGATTTTCTTCCCATATATCATCTTCTGCCGAGGCTGCACCACAAAACTTTCAGCTATACAGTTGGGAAACTTTTCAACCTGTTGGTGGTCTACTTCCTCAACCAGAATGGACAGCATGGGATCAAACGGTTGAGTATTGTGCTTTTGCATATCAGAAATACATTGTCATATCTTCTTTGCGCCCGCAATATAGATATCTGGGAGATGTCGCAATTCCTTACGCTACTGGTGCAGTTTGGCATAGGCGACAGTTATTTGTCGTTACACCAACCACAATTGAATGTGTTTTTGTGGATGCTGGAGTTGCACCTGTTGATATTGAAACCAGAAAGAGGAAAGAGGAAATGAAGCAAAAGGAGGCACAATCAAGAGCCCTTGCTGAGCATGGTGAACTAGCACTAATCGCAGTTGAAGGTCCGCAATCTGTTACACAAGAAAGGATAGCACTGAGGCCGCCGATGCTACAGGTGGTGCGATTAGCCTCATTTCAGCATGCTCCATCTGTGCCTCCTTTCTTAACATTATCGAAACAGTCTAGAAATGACGGGGACAGTTCAGGGATACTAAAAGAGTTTGAAGAAAGAAGGGTTAATGAGATAGCTGTTGGTGGTGGAGGGGTCTCAGTGGCAGTTACTCGTTTTCCAACAGAGCAGAAACGGCCTGTTGGGCCTCTAGTTGTGGTGGGTGTCAGAGATGGCGTTCTTTGGCTAATTGATAGATACATGAGTGCTCATGCCTTATCCCTTAGCCACCCTGGGATCCGTTGTCGGTGTCTTGCTGCTTATGGAGATGCAGTTAGTGCTGTCAAGTGGGCAAGTAGGCTTGGCAGAGAACATCATGATGATTTAGCACAGTTTTTGCTAGGCATGGGTTATGCTACTGAGGCACTTCATTTGCCTGGAATATCTAAGAGGTTGGAGTTTGATTTAGCCATGCAGAGCAGTGATTTGAAAAGAGCTCTTCAGTGCCTGCTTACAATGAGCAACAGCAGGGACATAGGCTGTCCAAGGAATAGTGAAGCTGAAATTGCTCGGGAAGCCCTTAAGAGGTTGGGTGCTGCAGCTTCTGTAAAGGGAGCTTTGCAGGGTCATGAATTAAGAGGACAAGCTCTGCGCTTAGCCAATCATGGAGAGTTGACGCGGCTAAGTAATTTGGTAAACAATTTAATATCAGTTGGCTCTGGACGTGAAGCAGCATTTGCAGCTGCAGTTTTGGGAGACAATGCCCTCATGGAGAAGGCGTGGCAGGAAACTGGAATGCTCGCTGAGGCTGTGCTTCATGCCCATGCTCATGGGCGACCAACATTGAAGAACTTGGTTCAGACTTGGAATAACATGCTACAGAAGGAGGTTGACCACACACCAACAACAAAAACGGATGCTGCTGCTGCATTTTTGGCTTCTCTGGAGGAACCCAAGCTTACAAGTTTAGCAGATGCTGCCAAGAAACCACCGATTGAAATTCTTCCTCCCGGGATGCCGTCTCTTTCAGCTCCTCCTATTTCTATGCCAAAGAAAACAGCTCCAGGGGCTCAGAATTCACTGCAGCAACCAGCCAAGCCGTTGATGTTAGAAGCAGCAGCCCCTACACCCACACCAGCACCTTCAGGTGCTCCACAAGCACAACAATCAGAATCAGGATCAGATGATAAGCCTCCCGCTTCATCATCAGAGAGTGATCCAGCTAATCCAGTTTCGGCTGCTTCGGGAGAAAGTGTCCCCGTAACTTCTACGGATAATGGAGCACCATTAGATGCCCCACCTCAAGCGCCCCAATCAGGGGTTCCATCCCAAGAGCCCCAATCAGAGGCCCCATCGCAAGCGCCCCAGTCAGAGGCTGCATCGCAAGCAACCCAGTCAGAGGCTTCTGCATCCCAAGCGCCCCAATCAGAGGCCAATCCGCCCCAAGCACCCCAATCTGAGGCACCGCCCCAGTCAGATGCCCCATTTCAAGCTCCACAACCGGAAGCACCCCAACCTGCCGTCCCAACCCAGACGACCTCATTACAACCCCAGGTGCCACCCGTTCAGCAAACCAATGCTCCAAAAAGATTGCCACTAGATGAGGATTTTTTCCGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

866

Amino Acids

92.32

Weight (kDa)

6.03

Isoelectric Point (pI)

61.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_COPA_B PF23953 435 - 614 1.6e-06 COPA/B TPR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2500
AccB1I GGYRCC 2 cut(s) 2437, 2530
AccI GTMKAC 2 cut(s) 176, 604
AccII CGCG 1 cut(s) 1654
AciI CCGC 9 cut(s) 251, 429, 698, 932, 968, 1654, 2172, 2414, 2441
AclWI GGATC 6 cut(s) 223, 642, 1267, 1280, 2161, 2186
AcsI RAATTY 3 cut(s) 192, 1974, 2050
AcuI CTGAAG 2 cut(s) 1460, 2103
AfaI GTAC 2 cut(s) 14, 285
AflII CTTAAG 1 cut(s) 1556
AflIII ACRYGT 1 cut(s) 146
AjiI CACGTC 1 cut(s) 1700
AjnI CCWGG 4 cut(s) 1267, 1411, 2038, 2526
AjuI GAANNNNNNNTTGG 2 cut(s) 2280, 2312
Alw21I GWGCWC 5 cut(s) 382, 1246, 1474, 2128, 2260
Alw26I GTCTC 3 cut(s) 1138, 1725, 2001
AlwI GGATC 6 cut(s) 223, 642, 1267, 1280, 2161, 2186
AlwNI CAGNNNCTG 5 cut(s) 248, 1483, 1580, 1952, 2351
Ama87I CYCGRG 2 cut(s) 1545, 1985
AoxI GGCC 6 cut(s) 59, 965, 1172, 1181, 2322, 2406
ApoI RAATTY 3 cut(s) 192, 1974, 2050
Asp700I GAANNNNTTC 2 cut(s) 585, 1977
AspLEI GCGC 5 cut(s) 696, 1629, 2284, 2338, 2395
AspS9I GGNCC 3 cut(s) 929, 1181, 2323
AsuC2I CCSGG 3 cut(s) 356, 1986, 1987
AsuHPI GGTGA 1 cut(s) 914
AvaI CYCGRG 2 cut(s) 1545, 1985
AvaII GGWCC 1 cut(s) 929
BaeI ACNNNNGTAYC 2 cut(s) 1225, 1258
BamHI GGATCC 1 cut(s) 1272
BanI GGYRCC 2 cut(s) 2437, 2530
BanII GRGCYC 4 cut(s) 889, 1474, 2047, 2313
Bbv12I GWGCWC 5 cut(s) 382, 1246, 1474, 2128, 2260
BbvCI CCTCAGC 1 cut(s) 1779
BccI CCATC 6 cut(s) 302, 506, 1021, 1202, 2308, 2335
BceAI ACGGC 4 cut(s) 1187, 1978, 2059, 2480
BciT130I CCWGG 4 cut(s) 1269, 1413, 2040, 2528
BciVI GTATCC 2 cut(s) 136, 1068
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 3 cut(s) 356, 1986, 1987
BcoDI GTCTC 3 cut(s) 1138, 1725, 2001
BfaI CTAG 6 cut(s) 170, 908, 1050, 1187, 1376, 2573
BfmI CTRYAG 8 cut(s) 240, 273, 336, 977, 1572, 1719, 1853, 2057
BfoI RGCGCY 3 cut(s) 2285, 2339, 2396
BfrI CTTAAG 1 cut(s) 1556
BfuAI ACCTGC 1 cut(s) 2500
BfuI GTATCC 2 cut(s) 136, 1068
BglI GCCNNNNNGGC 1 cut(s) 1180
BglII AGATCT 1 cut(s) 332
BlpI GCTNAGC 2 cut(s) 894, 1630
Bme1390I CCNGG 7 cut(s) 356, 1269, 1413, 1986, 1987, 2040, 2528
Bme18I GGWCC 1 cut(s) 929
BmeT110I CYCGRG 2 cut(s) 1545, 1985
BmgBI CACGTC 1 cut(s) 1700
BmgT120I GGNCC 3 cut(s) 929, 1181, 2323
BmiI GGNNCC 6 cut(s) 1274, 1926, 2298, 2325, 2439, 2532
BmrFI CCNGG 7 cut(s) 356, 1269, 1413, 1986, 1987, 2040, 2528
BmrI ACTGGG 3 cut(s) 2335, 2362, 2440
BmuI ACTGGG 3 cut(s) 2335, 2362, 2440
BpmI CTGGAG 3 cut(s) 834, 1940, 2022
Bpu10I CCTNAGC 2 cut(s) 1259, 1779
Bpu1102I GCTNAGC 2 cut(s) 894, 1630
BpuEI CTTGAG 1 cut(s) 2262
BpuMI CCSGG 3 cut(s) 356, 1986, 1987
BsaI GGTCTC 1 cut(s) 1138
BsaJI CCNNGG 7 cut(s) 543, 1267, 1268, 1520, 1985, 2039, 2526
BsaWI WCCGGW 1 cut(s) 2476
BsaXI ACNNNNNCTCC 8 cut(s) 1293, 1323, 1739, 1769, 2214, 2244, 2539, 2569
Bse1I ACTGG 7 cut(s) 104, 745, 1771, 2204, 2341, 2368, 2446
BseBI CCWGG 4 cut(s) 1269, 1413, 2040, 2528
BseDI CCNNGG 7 cut(s) 543, 1267, 1268, 1520, 1985, 2039, 2526
BseGI GGATG 6 cut(s) 310, 814, 1897, 1995, 2300, 2384
BseMII CTCAG 7 cut(s) 885, 953, 1149, 1386, 1770, 2060, 2424
BseNI ACTGG 7 cut(s) 104, 745, 1771, 2204, 2341, 2368, 2446
BseRI GAGGAG 2 cut(s) 390, 1998
BseYI CCCAGC 1 cut(s) 482
BsgI GTGCAG 2 cut(s) 534, 765
Bsh1236I CGCG 1 cut(s) 1654
BshFI GGCC 6 cut(s) 61, 967, 1174, 1183, 2324, 2408
BshNI GGYRCC 2 cut(s) 2437, 2530
BsiHKAI GWGCWC 5 cut(s) 382, 1246, 1474, 2128, 2260
BsiHKCI CYCGRG 2 cut(s) 1545, 1985
BsiSI CCGG 3 cut(s) 356, 1986, 2477
BslFI GGGAC 4 cut(s) 1076, 1520, 2216, 2483
BsmAI GTCTC 3 cut(s) 1138, 1725, 2001
BsmBI CGTCTC 1 cut(s) 2001
BsmFI GGGAC 4 cut(s) 1076, 1520, 2216, 2483
BsmI GAATGC 2 cut(s) 487, 1776
BsnI GGCC 6 cut(s) 61, 967, 1174, 1183, 2324, 2408
Bso31I GGTCTC 1 cut(s) 1138
BsoBI CYCGRG 2 cut(s) 1545, 1985
Bsp1286I GDGCHC 8 cut(s) 382, 889, 1246, 1474, 2047, 2128, 2260, 2313
Bsp143I GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
Bsp1720I GCTNAGC 2 cut(s) 894, 1630
BspACI CCGC 9 cut(s) 251, 429, 698, 932, 968, 1654, 2172, 2414, 2441
BspANI GGCC 6 cut(s) 61, 967, 1174, 1183, 2324, 2408
BspCNI CTCAG 7 cut(s) 886, 954, 1148, 1387, 1771, 2059, 2425
BspFNI CGCG 1 cut(s) 1654
BspHI TCATGA 2 cut(s) 1351, 1603
BspLI GGNNCC 6 cut(s) 1274, 1926, 2298, 2325, 2439, 2532
BspMAI CTGCAG 5 cut(s) 244, 277, 1576, 1723, 2061
BspMI ACCTGC 1 cut(s) 2500
BspPI GGATC 6 cut(s) 223, 642, 1267, 1280, 2161, 2186
BspQI GCTCTTC 1 cut(s) 1479
BspT107I GGYRCC 2 cut(s) 2437, 2530
BspTI CTTAAG 1 cut(s) 1556
BspTNI GGTCTC 1 cut(s) 1138
BsrI ACTGG 7 cut(s) 104, 745, 1771, 2204, 2341, 2368, 2446
BssECI CCNNGG 7 cut(s) 543, 1267, 1268, 1520, 1985, 2039, 2526
BssMI GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
BssT1I CCWWGG 1 cut(s) 1520
Bst2UI CCWGG 4 cut(s) 1269, 1413, 2040, 2528
Bst4CI ACNGT 6 cut(s) 575, 643, 765, 1047, 1067, 1368
Bst6I CTCTTC 1 cut(s) 1479
BstAFI CTTAAG 1 cut(s) 1556
BstAPI GCANNNNNTGC 4 cut(s) 248, 1313, 1712, 1952
BstC8I GCNNGC 7 cut(s) 368, 698, 1008, 1484, 1776, 2334, 2361
BstF5I GGATG 6 cut(s) 310, 814, 1897, 1995, 2300, 2384
BstFNI CGCG 1 cut(s) 1654
BstH2I RGCGCY 3 cut(s) 2285, 2339, 2396
BstHHI GCGC 5 cut(s) 696, 1629, 2284, 2338, 2395
BstKTI GATC 7 cut(s) 31, 218, 335, 637, 1275, 2156, 2194
BstMAI GTCTC 3 cut(s) 1138, 1725, 2001
BstMBI GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
BstNI CCWGG 4 cut(s) 1269, 1413, 2040, 2528
BstNSI RCATGY 3 cut(s) 150, 1010, 1852
BstSCI CCNGG 7 cut(s) 354, 1267, 1411, 1984, 1985, 2038, 2526
BstSFI CTRYAG 8 cut(s) 240, 273, 336, 977, 1572, 1719, 1853, 2057
BstUI CGCG 1 cut(s) 1654
BstX2I RGATCY 2 cut(s) 332, 1272
BstXI CCANNNNNNTGG 1 cut(s) 1805
BstYI RGATCY 2 cut(s) 332, 1272
BsuI GTATCC 2 cut(s) 136, 1068
BsuRI GGCC 6 cut(s) 61, 967, 1174, 1183, 2324, 2408
BtgZI GCGATG 2 cut(s) 2313, 2340
BtrI CACGTC 1 cut(s) 1700
BtsCI GGATG 6 cut(s) 310, 814, 1897, 1995, 2300, 2384
BtsI GCAGTG 4 cut(s) 127, 355, 1462, 2054
BtsIMutI CAGTG 7 cut(s) 127, 355, 959, 1143, 1462, 1484, 2054
BveI ACCTGC 1 cut(s) 2500
Cac8I GCNNGC 7 cut(s) 368, 698, 1008, 1484, 1776, 2334, 2361
CaiI CAGNNNCTG 5 cut(s) 248, 1483, 1580, 1952, 2351
CciI TCATGA 2 cut(s) 1351, 1603
CfoI GCGC 5 cut(s) 696, 1629, 2284, 2338, 2395
Cfr13I GGNCC 3 cut(s) 929, 1181, 2323
Cfr9I CCCGGG 1 cut(s) 1985
CseI GACGC 1 cut(s) 1660
Csp6I GTAC 2 cut(s) 13, 284
CviQI GTAC 2 cut(s) 13, 284
DpnI GATC 7 cut(s) 30, 217, 334, 636, 1274, 2155, 2193
DpnII GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
Eam1104I CTCTTC 1 cut(s) 1479
EarI CTCTTC 1 cut(s) 1479
EciI GGCGGA 1 cut(s) 2403
Ecl136II GAGCTC 1 cut(s) 1472
Eco130I CCWWGG 1 cut(s) 1520
Eco24I GRGCYC 4 cut(s) 889, 1474, 2047, 2313
Eco31I GGTCTC 1 cut(s) 1138
Eco32I GATATC 2 cut(s) 67, 710
Eco47I GGWCC 1 cut(s) 929
Eco53kI GAGCTC 1 cut(s) 1472
Eco57I CTGAAG 2 cut(s) 1460, 2103
Eco88I CYCGRG 2 cut(s) 1545, 1985
EcoICRI GAGCTC 1 cut(s) 1472
EcoO109I RGGNCCY 1 cut(s) 2323
EcoRI GAATTC 2 cut(s) 192, 2050
EcoRII CCWGG 4 cut(s) 1267, 1411, 2038, 2526
EcoRV GATATC 2 cut(s) 67, 710
EcoT14I CCWWGG 1 cut(s) 1520
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 4 cut(s) 889, 1474, 2047, 2313
ErhI CCWWGG 1 cut(s) 1520
Esp3I CGTCTC 1 cut(s) 2001
FaqI GGGAC 4 cut(s) 1076, 1520, 2216, 2483
FauI CCCGC 2 cut(s) 705, 2179
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 2 cut(s) 176, 604
FokI GGATG 6 cut(s) 317, 821, 1904, 2002, 2287, 2371
FriOI GRGCYC 4 cut(s) 889, 1474, 2047, 2313
FspBI CTAG 6 cut(s) 170, 908, 1050, 1187, 1376, 2573
GlaI GCGC 5 cut(s) 695, 1628, 2283, 2337, 2394
GsaI CCCAGC 1 cut(s) 486
GsuI CTGGAG 3 cut(s) 834, 1940, 2022
HaeII RGCGCY 3 cut(s) 2285, 2339, 2396
HaeIII GGCC 6 cut(s) 61, 967, 1174, 1183, 2324, 2408
HapII CCGG 3 cut(s) 356, 1986, 2477
HgaI GACGC 1 cut(s) 1660
HhaI GCGC 5 cut(s) 696, 1629, 2284, 2338, 2395
Hin6I GCGC 5 cut(s) 694, 1627, 2282, 2336, 2393
HinP1I GCGC 5 cut(s) 694, 1627, 2282, 2336, 2393
HincII GTYRAC 2 cut(s) 1650, 1867
HindII GTYRAC 2 cut(s) 1650, 1867
HindIII AAGCTT 1 cut(s) 1931
HinfI GANTC 3 cut(s) 113, 173, 2147
HpaII CCGG 3 cut(s) 356, 1986, 2477
HphI GGTGA 1 cut(s) 914
Hpy166II GTNNAC 7 cut(s) 13, 177, 605, 905, 1650, 1672, 1867
Hpy8I GTNNAC 7 cut(s) 13, 177, 605, 905, 1650, 1672, 1867
HpyAV CCTTC 5 cut(s) 920, 1092, 1744, 1852, 2127
HpyCH4III ACNGT 6 cut(s) 575, 643, 765, 1047, 1067, 1368
HpyCH4IV ACGT 2 cut(s) 282, 1699
HpySE526I ACGT 2 cut(s) 282, 1699
HspAI GCGC 5 cut(s) 694, 1627, 2282, 2336, 2393
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
LguI GCTCTTC 1 cut(s) 1479
LmnI GCTCC 9 cut(s) 377, 1015, 1589, 2011, 2041, 2131, 2255, 2473, 2560
MaeI CTAG 6 cut(s) 170, 908, 1050, 1187, 1376, 2573
MaeII ACGT 2 cut(s) 282, 1699
MaeIII GTNAC 7 cut(s) 95, 152, 350, 775, 940, 1144, 2236
MalI GATC 7 cut(s) 30, 217, 334, 636, 1274, 2155, 2193
MboI GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
MboII GAAGA 8 cut(s) 483, 513, 528, 678, 1103, 1466, 1834, 1970
MfeI CAATTG 2 cut(s) 105, 789
MflI RGATCY 2 cut(s) 332, 1272
MhlI GDGCHC 8 cut(s) 382, 889, 1246, 1474, 2047, 2128, 2260, 2313
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 1181, 1410
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 585, 1977
MseI TTAA 6 cut(s) 326, 1031, 1104, 1557, 1610, 1679
MslI CAYNNNNRTG 3 cut(s) 1353, 1797, 1803
MspA1I CMGCKG 3 cut(s) 248, 257, 1718
MspCI CTTAAG 1 cut(s) 1556
MspI CCGG 3 cut(s) 356, 1986, 2477
MspR9I CCNGG 7 cut(s) 356, 1269, 1413, 1986, 1987, 2040, 2528
MunI CAATTG 2 cut(s) 105, 789
Mva1269I GAATGC 2 cut(s) 487, 1776
MvaI CCWGG 4 cut(s) 1269, 1413, 2040, 2528
MvnI CGCG 1 cut(s) 1654
NciI CCSGG 3 cut(s) 356, 1986, 1987
NdeII GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
NlaIV GGNNCC 6 cut(s) 1274, 1926, 2298, 2325, 2439, 2532
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 3 cut(s) 95, 152, 350
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 150, 1010, 1852
PaeI GCATGC 1 cut(s) 1010
PagI TCATGA 2 cut(s) 1351, 1603
PasI CCCWGGG 1 cut(s) 1268
PciI ACATGT 1 cut(s) 146
PciSI GCTCTTC 1 cut(s) 1479
PctI GAATGC 2 cut(s) 487, 1776
PdmI GAANNNNTTC 2 cut(s) 585, 1977
PfeI GAWTC 2 cut(s) 113, 2147
PflFI GACNNNGTC 1 cut(s) 356
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PscI ACATGT 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 1474
Psp6I CCWGG 4 cut(s) 1267, 1411, 2038, 2526
PspFI CCCAGC 1 cut(s) 482
PspGI CCWGG 4 cut(s) 1267, 1411, 2038, 2526
PspN4I GGNNCC 6 cut(s) 1274, 1926, 2298, 2325, 2439, 2532
PspPI GGNCC 3 cut(s) 929, 1181, 2323
PstI CTGCAG 5 cut(s) 244, 277, 1576, 1723, 2061
PstNI CAGNNNCTG 5 cut(s) 248, 1483, 1580, 1952, 2351
PsuI RGATCY 2 cut(s) 332, 1272
PsyI GACNNNGTC 1 cut(s) 356
PvuII CAGCTG 3 cut(s) 248, 257, 1718
RsaI GTAC 2 cut(s) 14, 285
RsaNI GTAC 2 cut(s) 13, 284
RseI CAYNNNNRTG 3 cut(s) 1353, 1797, 1803
SacI GAGCTC 1 cut(s) 1474
SapI GCTCTTC 1 cut(s) 1479
SaqAI TTAA 6 cut(s) 326, 1031, 1104, 1557, 1610, 1679
Sau3AI GATC 7 cut(s) 28, 215, 332, 634, 1272, 2153, 2191
Sau96I GGNCC 3 cut(s) 929, 1181, 2323
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 7 cut(s) 356, 1269, 1413, 1986, 1987, 2040, 2528
SduI GDGCHC 8 cut(s) 382, 889, 1246, 1474, 2047, 2128, 2260, 2313
SfcI CTRYAG 8 cut(s) 240, 273, 336, 977, 1572, 1719, 1853, 2057
SfiI GGCCNNNNNGGCC 1 cut(s) 1180
SinI GGWCC 1 cut(s) 929
SmaI CCCGGG 1 cut(s) 1987
SmiMI CAYNNNNRTG 3 cut(s) 1353, 1797, 1803
SmlI CTYRAG 2 cut(s) 1556, 2277
SmoI CTYRAG 2 cut(s) 1556, 2277
SphI GCATGC 1 cut(s) 1010
SsiI CCGC 9 cut(s) 251, 429, 698, 932, 968, 1654, 2172, 2414, 2441
SspI AATATT 1 cut(s) 322
SspMI CTAG 6 cut(s) 170, 908, 1050, 1187, 1376, 2573
SstI GAGCTC 1 cut(s) 1474
StyD4I CCNGG 7 cut(s) 354, 1267, 1411, 1984, 1985, 2038, 2526
StyI CCWWGG 1 cut(s) 1520
TaaI ACNGT 6 cut(s) 575, 643, 765, 1047, 1067, 1368
TaiI ACGT 2 cut(s) 285, 1702
TaqI TCGA 5 cut(s) 190, 221, 400, 507, 1040
TauI GCSGC 3 cut(s) 254, 970, 1657
TfiI GAWTC 2 cut(s) 113, 2147
Tru1I TTAA 6 cut(s) 326, 1031, 1104, 1557, 1610, 1679
Tru9I TTAA 6 cut(s) 326, 1031, 1104, 1557, 1610, 1679
TscAI CASTG 7 cut(s) 127, 355, 966, 1143, 1462, 1484, 2061
TseFI GTSAC 3 cut(s) 95, 152, 350
Tsp45I GTSAC 3 cut(s) 95, 152, 350
TspDTI ATGAA 6 cut(s) 194, 875, 1394, 1620, 1781, 2166
TspGWI ACGGA 3 cut(s) 1265, 1904, 2261
TspMI CCCGGG 1 cut(s) 1985
TspRI CASTG 7 cut(s) 127, 355, 966, 1143, 1462, 1484, 2061
Tth111I GACNNNGTC 1 cut(s) 356
Vha464I CTTAAG 1 cut(s) 1556
VpaK11BI GGWCC 1 cut(s) 929
XapI RAATTY 3 cut(s) 192, 1974, 2050
XbaI TCTAGA 1 cut(s) 1049
XceI RCATGY 3 cut(s) 150, 1010, 1852
XmaI CCCGGG 1 cut(s) 1985
XmiI GTMKAC 2 cut(s) 176, 604
XmnI GAANNNNTTC 2 cut(s) 585, 1977
XspI CTAG 6 cut(s) 170, 908, 1050, 1187, 1376, 2573
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.