Rh6DG254300

CONTAINS InterPro DOMAIN s WD40 repeat-like (InterPro IPR011046), WD40 repeat, region (InterPro IPR017986), WD40 repeat (InterPro IPR001680), WD40 YVTN repeat-like (InterPro IPR015943)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008060
Physical Location & Seq
Reverse (-)
44454326 .. 44526578
72253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG254300.1

Sequence Viewer

Length: 4200 bp
ATGTTGCGGTTAAGAGCTTTCCGGCCGACGAACGACAAGATCGTCAAGATTCAGTTGCATCCAACGCATCCATGGCTCGTCACCGCCGATGCCTCCGATCACGTCTCCGTTTGGAATTGGGAACATCGCCAGGTGATTTACGAGCTCAAAGCTGGCGGTGTCGATCACCGGCGTTTGGTCGGAGCCAAGTTGGAGAAGCTCGCCGAGGGTGAAACAGACTCCAAGGGGAAACCCACTGAAGCCATTCGTGGAGGGAGTGTTAAGCAGGTAAACTTCTATGATGATGACGTGCGCTTTTGGCAACTTTGGCGTAACCGTTCTGCAGCTGCTGAGGCTCCATCGGCTGTCAATAATGTAGCATCACCATTTAATTCTCCTGCTCCAACAACCAAAGGAAGACACTTTCTTGTCATCTGTTGTGAAAACAAAGCCATATTTTTGGACTTGGTGACAATGCGTGGCCGTGATGTACCAAAGCAGGAACTTGACAACAAATCACTTCTCTGTATGGAATTTCTCTCTAGATCTGCTGTTGGTGATGTTCCTCTTGTTGCTTTTGGTGGATCAGATGGTGTTATTAGAGTACTTTCAATGATGACATGGAAGCTTGTAAGAAGATATACCGGAGGTCATAAAGGATCCATAGCTTGTTTGATGACATTTGTGTCCTCTTCTGGTGAGGCTCTTTTGGTCTCGGGGGGTAATGATGGTTTGCTTGTAGTTTGGAGTGCAGATCATGCTCAAGATTCAAGAGAACTCGTACCCAAGCTCAGTATAAAGGCACATGATGGTGGGGTTGTCGCTGTTGAACTTTCTAGAGTGATCGGAGCAGCTCCTCAGCTTATCTCAATTGGTGCAGATAAGACATTAGCTATATGGGACACAATTTCTTTTAAGGAGCTGCGGCGCATTAAACCTGTTCCAAAAATGGCTTGCCATAGTGTAGCATCCTGGTGCCACCCTCGTGCTCCAAACCTTGATATACTGACTTGCGTTAAAGATTCCAATATATGGGCCATCGAACACCCAACTTACTCTGCTCTGACAAGGCCGTTGTGTGAACTTTCTTCTCTGATCCCTCCACATGTGATCGCTCCCAATAAGAAAATTAGGGTTTACTGTATGGTTGCACATCCTTTACAGCCTCACCTGGTTGCTACTGGATCCAATGTCGGTATCATTATCAGTGAATTTGACCCCAGATCTCTTCCAGCTGTAGCTCCCCTACTAACACCATCAGGAAGTCGAGAGCATTCTGCTGTGTATGTTATTGAAAGGGAACTGAAGCTGCTAAACTTTCAGTTATCCCAGACAGCAAATCCAACTCTTGGAAATAATGCTTCTTTGTCGGAGACTGGAAGGCTTAGGGGAGATTCACTTGAAACATTACATGTCAAACAGATTAAGAAGCACATTAGTACGCCTGTTCCACATGATTCATATTCAATTCTTTCTGTAAGCAGTTCGGGAAAGTATCTTGCGATTGTGTGGCCAGATATCCCTTACTTCTCTATTTACAAAGTTAGTGACTGGTCCATCGTTGATTCAGGCAGTGCAAGGCTTTTGGCTTGGGATACATGCCGTGATAGATTTGCCATACTGGAATCGTCATTGCCTCCTCGAATTCCTGTAGTTCCGAAGGGCAGTTCATCGAGAAAAGCAAGAGAAGCAGCTGCAGCTGCAGCACAAGCTGCTGCTGCAGCTGCATCAGCTGCTTCTGCAGCCAATGTACAAGTTCGTATCTTGCTGGATGATGGCACCTCAAATATTTTAATGAGATCCATAGGTGGTCGCAGTGAACCGGTCATTGGCTTGCACGGAGGGGCACTTCTTGGTGTTGCATATCGAACATCTCGGAGAATTAGCCCAGTTGCTGCAACTGCTATTTCAACAATTCAATCTATGCCCCTGTCTGGATTTGGAAGTGCTGGTGTTTCTTCTTTTTCCACTTTTGACGATGGCTTTTCTTCTCACAAGTCTCCAGCTGAGGCTACACCCCAAAACTTTCAGCTATATAGTTGGGAAACTTTTCAACCTGTTGGTGGTCTACTTCCTCAGCCAGAATGGACAGCATGGGATCAAACGGTTGAGTATTGCGCTTTTGCATATCAGAAATACATCGTCATATCTTCTTTGCGCCCACAATATAGATACCTGGGCGATGTTGCAATTCCTTATGCTACTGGTGCTGTATGGCATCGGCGACAGTTGTTTGTTGTTACCCCAACCACCATCGAATGTGTTTTTGTGGATGCTGGAGTTGCAGCGATTGATATCGAAACAAAAAAGAGGAAAGAGGAGATGATGCTGAAGGAGGTACAAGCAAAAGCCCTTGCTGAGCATGGTGATTTGGCACTAATTGCGGTTGATGGTCCTCAATCTGCTACCCAGGAAAGGATAGCATTGAGGCCCCCAATGCTACAGGTGGTGCGATTAGCCTCATTTCAGCATGCTCCATCTGTGCCCCCATTCTTAACCTTGTCAAAGCAATCTAGAGTTGATGGGGATGATTCTGGGATTGCAGAAGAAAGAAAGGTTAACGAGGTGGCTGTTGGTGGTGGAGGGGTCTCAGTTGCAGTTACTCGTTTTCCATTAGAGCAGAAACGACCTGTAGGACCTCTGGTTGTGGTGGGTGTCAAAGATGGTGTTCTTTGGCTAATTGATAGATACATGAGTGCTCATGCCTTATCCCTCAGCCACCCCGGTATCCGCTGCCGGTGCCTTGCTGCTTATGGAGATGCTGTTAGTGCTGTGAAATGGGCAAGTAGGCTTGGTAGAGAACATCATGATGATTTAGCCCAGTTTCTGCTAGGGATGGGTTATGCTACTGAGGCACTTCATTTGCCTGGAATCTCTAAGAGGTTGGAGTTTGATTTGGCCATGCAGAGCAGTGATTTGAAAAGAGCTCTCCAGTGCCTTCTCACAATGAGCAACAGCAGGGACTTGGGGCAAGATAATTCAGGATTTGATTTGAAGGACATTCTTACTATAACAACTAAGAAAGAGAATATATTGGAAGCTGTCCAAGGAATAGTGAAATTTACAAAGGAGTTTTTGGATCTTATTGATGCCGCAGATGCTACTGGACAGGCTGAAATTGCTCGGGAGGCTCTTAAGAGGTTGGCTGCTGCGGCTTCTGTGAAAGGAGCTTTACAGGGTCATGAATTAAGAGGACAAGCTCTTCGGTTAGCCAATCATGGAGAGTTGACACGGCTTAGTAATTTGGTAAACAATTTAATATCCATTGGTGCTGGACGGGAAGCAGCATTCGCAGCTGCAGTTTTGGGAGACAATGCCCTCATGGAGAAGGCTTGGCAGGATACTGGAATGCTTGCTGAGGCTGTGCTTCATGCCCATGCTCATGGGAGACCAACATTGAAGAACTTGGTTCAGGCATGGAATAAAATGCTGCAAAAGGAGGTTGAGCACACCCCATTAGAAAAAACGGATGCTGCTGCAGCATTTTTGGCTTCTCTGGAGGAACCCAAGCTTACAAGTTTAGCAGATGCTGCCAAGAAACCACCAATTGAAATTCTTCCTCCAGGGATGCCATCTCTTACAGTTGCTATGGCTGTTCAAAAGAAACCGCCTCCAGGGGCCCAGAATTCACAGCAGCAACCGGGCAAGCCACTGCTGTTAGAAGCAGCCCCTGCTACCACACCAGCCCCTTCAAGTCTGCCACAACAATCTGAATCTGGTGAACCAACTTCAGACAATAATCCCCCTGTTTCATCAACAGACAGTAATCCAGCTCCAGTTGCTTCTGGAGAAAGTGTCCTGGAAAATTCTAAGAATGATGTGGCACTATCAGACACGCCATCGGATGCGCCACCTCAAACCTCACAATCTGATGCCGCATCCCAAGGCCCACAACCTGATGCAACCCCATCCGAAGCGCCACAACCTGATGTCCCATCCCAAGCGCCGCAACCTGATGCCCCATCCCAAGCGCCGCAACCTGATACCCCATCCCAGGCACCGCCACCAGAAGCCCCATCCCAGGTGCCGCCACCAGAAGCCCCATCCCAAGCTCCGCAACCAGAGGCCTCATCCCTGGCGCCGCAACCAGAGGCCTCATCTCAAGTGCCACAACCACAGGTCCCAATCCAGACACCACCATTACAAACCCAACCCCAGGTGCCAGCGATTCAGGAAACCAAACAAGCAAGATTGCCAATAAGTGATGATTGGCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1399

Amino Acids

150.34

Weight (kDa)

6.54

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 181 - 296 2.9e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 182 - 312 7.2e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 189 - 293 3.3e-06 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 228 - 305 9e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 41, 664
Acc36I ACCTGC 1 cut(s) 256
AccB1I GGYRCC 7 cut(s) 954, 1757, 2718, 3976, 4003, 4057, 4138
AccB7I CCANNNNNTGG 2 cut(s) 1011, 1328
AccI GTMKAC 1 cut(s) 2047
AclWI GGATC 9 cut(s) 571, 633, 646, 1069, 1158, 1171, 1773, 2085, 3066
AcoI YGGCCR 4 cut(s) 23, 460, 1490, 2877
AcsI RAATTY 7 cut(s) 512, 1190, 1623, 3038, 3531, 3604, 3784
AcuI CTGAAG 4 cut(s) 258, 1304, 2330, 3693
AcyI GRCGYC 1 cut(s) 4058
AfaI GTAC 6 cut(s) 471, 585, 762, 1420, 1731, 2319
AflII CTTAAG 1 cut(s) 3113
AflIII ACRYGT 2 cut(s) 1084, 1390
AgeI ACCGGT 1 cut(s) 1801
AjiI CACGTC 2 cut(s) 103, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 1791, 1823
AleI CACNNNNGTG 1 cut(s) 963
Alw21I GWGCWC 5 cut(s) 147, 970, 2680, 2908, 3429
Alw26I GTCTC 7 cut(s) 109, 697, 1346, 1983, 2572, 3282, 3361
AlwI GGATC 9 cut(s) 571, 633, 646, 1069, 1158, 1171, 1773, 2085, 3066
AlwNI CAGNNNCTG 5 cut(s) 329, 1874, 2806, 3509, 3650
Ama87I CYCGRG 2 cut(s) 694, 3102
ApaI GGGCCC 1 cut(s) 3601
ApoI RAATTY 7 cut(s) 512, 1190, 1623, 3038, 3531, 3604, 3784
ArsI GACNNNNNNTTYG 2 cut(s) 3165, 3197
AsiGI ACCGGT 1 cut(s) 1801
Asp700I GAANNNNTTC 3 cut(s) 243, 2028, 3534
AspLEI GCGC 9 cut(s) 294, 909, 2099, 2139, 3829, 3898, 3925, 3952, 4060
AspS9I GGNCC 9 cut(s) 1014, 1533, 2372, 2409, 2615, 3597, 3598, 3866, 4099
AsuC2I CCSGG 2 cut(s) 2703, 3621
AvaI CYCGRG 2 cut(s) 694, 3102
AvaII GGWCC 4 cut(s) 1533, 2372, 2615, 4099
BaeGI GKGCMC 3 cut(s) 1828, 2466, 3601
BaeI ACNNNNGTAYC 4 cut(s) 2659, 2689, 2692, 2722
BalI TGGCCA 2 cut(s) 1492, 2879
BamHI GGATCC 2 cut(s) 638, 1163
BanI GGYRCC 7 cut(s) 954, 1757, 2718, 3976, 4003, 4057, 4138
BanII GRGCYC 3 cut(s) 147, 2908, 3601
BauI CACGAG 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 5 cut(s) 147, 970, 2680, 2908, 3429
BbvCI CCTCAGC 6 cut(s) 330, 837, 1986, 2055, 2693, 3336
BceAI ACGGC 4 cut(s) 447, 1036, 1566, 3227
BciVI GTATCC 3 cut(s) 1567, 2717, 3313
BcnI CCSGG 2 cut(s) 2703, 3621
BcoDI GTCTC 7 cut(s) 109, 697, 1346, 1983, 2572, 3282, 3361
BfaI CTAG 4 cut(s) 522, 816, 2493, 2810
BfoI RGCGCY 4 cut(s) 3899, 3926, 3953, 4061
BfrI CTTAAG 1 cut(s) 3113
BfuAI ACCTGC 1 cut(s) 256
BfuI GTATCC 3 cut(s) 1567, 2717, 3313
BglII AGATCT 2 cut(s) 524, 1202
BlpI GCTNAGC 1 cut(s) 2337
BmcAI AGTACT 1 cut(s) 585
Bme18I GGWCC 4 cut(s) 1533, 2372, 2615, 4099
BmeT110I CYCGRG 2 cut(s) 694, 3102
BmgBI CACGTC 2 cut(s) 103, 289
BmgT120I GGNCC 9 cut(s) 1014, 1533, 2372, 2409, 2615, 3597, 3598, 3866, 4099
BmrI ACTGGG 2 cut(s) 1862, 2794
BmuI ACTGGG 2 cut(s) 1862, 2794
BpiI GAAGAC 1 cut(s) 403
BpmI CTGGAG 8 cut(s) 1965, 2277, 2894, 3497, 3525, 3576, 3738, 3786
Bpu10I CCTNAGC 7 cut(s) 330, 837, 1364, 1986, 2055, 2693, 3336
Bpu1102I GCTNAGC 1 cut(s) 2337
BpuEI CTTGAG 2 cut(s) 726, 4065
BpuMI CCSGG 2 cut(s) 2703, 3621
BsaBI GATNNNNATC 1 cut(s) 2273
BsaHI GRCGYC 1 cut(s) 4058
BsaI GGTCTC 3 cut(s) 697, 2572, 3361
BsaWI WCCGGW 2 cut(s) 623, 1801
BsaXI ACNNNNNCTCC 6 cut(s) 243, 273, 2727, 2757, 3759, 3789
Bse118I RCCGGY 3 cut(s) 168, 1801, 2715
Bse3DI GCAATG 1 cut(s) 1610
Bse8I GATNNNNATC 1 cut(s) 2273
BseJI GATNNNNATC 1 cut(s) 2273
BseMI GCAATG 1 cut(s) 1610
BseRI GAGGAG 3 cut(s) 825, 1608, 2312
BseSI GKGCMC 3 cut(s) 1828, 2466, 3601
BseX3I CGGCCG 1 cut(s) 23
BsgI GTGCAG 2 cut(s) 750, 876
Bsh1285I CGRYCG 1 cut(s) 26
BshNI GGYRCC 7 cut(s) 954, 1757, 2718, 3976, 4003, 4057, 4138
BshTI ACCGGT 1 cut(s) 1801
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 5 cut(s) 147, 970, 2680, 2908, 3429
BsiHKCI CYCGRG 2 cut(s) 694, 3102
BsiSI CCGG 7 cut(s) 22, 169, 624, 1802, 2703, 2716, 3620
BslFI GGGAC 4 cut(s) 893, 2954, 3896, 4085
BsmAI GTCTC 7 cut(s) 109, 697, 1346, 1983, 2572, 3282, 3361
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 4 cut(s) 893, 2954, 3896, 4085
BsmI GAATGC 3 cut(s) 1252, 3266, 3333
Bso31I GGTCTC 3 cut(s) 697, 2572, 3361
BsoBI CYCGRG 2 cut(s) 694, 3102
Bsp120I GGGCCC 1 cut(s) 3597
Bsp1286I GDGCHC 8 cut(s) 147, 970, 1828, 2466, 2680, 2908, 3429, 3601
Bsp1407I TGTACA 1 cut(s) 1729
Bsp1720I GCTNAGC 1 cut(s) 2337
Bsp19I CCATGG 1 cut(s) 71
BspHI TCATGA 2 cut(s) 2785, 3160
BspMAI CTGCAG 7 cut(s) 325, 1678, 1684, 1702, 1723, 3280, 3460
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 9 cut(s) 571, 633, 646, 1069, 1158, 1171, 1773, 2085, 3066
BspQI GCTCTTC 1 cut(s) 3186
BspT107I GGYRCC 7 cut(s) 954, 1757, 2718, 3976, 4003, 4057, 4138
BspTI CTTAAG 1 cut(s) 3113
BspTNI GGTCTC 3 cut(s) 697, 2572, 3361
BsrDI GCAATG 1 cut(s) 1610
BsrFI RCCGGY 3 cut(s) 168, 1801, 2715
BsrGI TGTACA 1 cut(s) 1729
BssAI RCCGGY 3 cut(s) 168, 1801, 2715
BssNI GRCGYC 1 cut(s) 4058
BssSI CACGAG 1 cut(s) 963
BssT1I CCWWGG 4 cut(s) 71, 222, 3025, 3862
Bst2BI CACGAG 1 cut(s) 963
Bst4CI ACNGT 6 cut(s) 317, 1121, 2086, 2208, 3562, 3743
Bst6I CTCTTC 3 cut(s) 676, 1212, 3186
BstACI GRCGYC 1 cut(s) 4058
BstAFI CTTAAG 1 cut(s) 3113
BstAPI GCANNNNNTGC 6 cut(s) 737, 1691, 1712, 2360, 3509, 3650
BstAUI TGTACA 1 cut(s) 1729
BstC8I GCNNGC 8 cut(s) 154, 201, 934, 1814, 2451, 3333, 3626, 4143
BstDSI CCRYGG 1 cut(s) 71
BstH2I RGCGCY 4 cut(s) 3899, 3926, 3953, 4061
BstHHI GCGC 9 cut(s) 294, 909, 2099, 2139, 3829, 3898, 3925, 3952, 4060
BstMAI GTCTC 7 cut(s) 109, 697, 1346, 1983, 2572, 3282, 3361
BstMCI CGRYCG 1 cut(s) 26
BstNSI RCATGY 4 cut(s) 1088, 1394, 1581, 2453
BstSLI GKGCMC 3 cut(s) 1828, 2466, 3601
BstV2I GAAGAC 1 cut(s) 403
BstX2I RGATCY 6 cut(s) 524, 638, 1163, 1202, 1778, 3058
BstXI CCANNNNNNTGG 2 cut(s) 439, 3362
BstYI RGATCY 6 cut(s) 524, 638, 1163, 1202, 1778, 3058
BstZI CGGCCG 1 cut(s) 23
BsuI GTATCC 3 cut(s) 1567, 2717, 3313
BtgI CCRYGG 1 cut(s) 71
BtgZI GCGATG 2 cut(s) 110, 2175
BtrI CACGTC 2 cut(s) 103, 289
BtsI GCAGTG 4 cut(s) 1558, 1801, 2896, 3629
BtsIMutI CAGTG 7 cut(s) 234, 1192, 1558, 1801, 2896, 2918, 3629
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 8 cut(s) 154, 201, 934, 1814, 2451, 3333, 3626, 4143
CaiI CAGNNNCTG 5 cut(s) 329, 1874, 2806, 3509, 3650
CciI TCATGA 2 cut(s) 2785, 3160
CfoI GCGC 9 cut(s) 294, 909, 2099, 2139, 3829, 3898, 3925, 3952, 4060
Cfr10I RCCGGY 3 cut(s) 168, 1801, 2715
Cfr13I GGNCC 9 cut(s) 1014, 1533, 2372, 2409, 2615, 3597, 3598, 3866, 4099
CsiI ACCWGGT 1 cut(s) 1149
Csp6I GTAC 6 cut(s) 470, 584, 761, 1419, 1730, 2318
CspAI ACCGGT 1 cut(s) 1801
CviQI GTAC 6 cut(s) 470, 584, 761, 1419, 1730, 2318
DinI GGCGCC 1 cut(s) 4059
DrdI GACNNNNNNGTC 2 cut(s) 41, 664
DseDI GACNNNNNNGTC 2 cut(s) 41, 664
EaeI YGGCCR 4 cut(s) 23, 460, 1490, 2877
EagI CGGCCG 1 cut(s) 23
Eam1104I CTCTTC 3 cut(s) 676, 1212, 3186
EarI CTCTTC 3 cut(s) 676, 1212, 3186
Ecl136II GAGCTC 2 cut(s) 145, 2906
EclXI CGGCCG 1 cut(s) 23
Eco130I CCWWGG 4 cut(s) 71, 222, 3025, 3862
Eco147I AGGCCT 2 cut(s) 4046, 4073
Eco24I GRGCYC 3 cut(s) 147, 2908, 3601
Eco31I GGTCTC 3 cut(s) 697, 2572, 3361
Eco32I GATATC 2 cut(s) 1498, 2275
Eco47I GGWCC 4 cut(s) 1533, 2372, 2615, 4099
Eco52I CGGCCG 1 cut(s) 23
Eco53kI GAGCTC 2 cut(s) 145, 2906
Eco57I CTGAAG 4 cut(s) 258, 1304, 2330, 3693
Eco88I CYCGRG 2 cut(s) 694, 3102
EcoICRI GAGCTC 2 cut(s) 145, 2906
EcoO109I RGGNCCY 4 cut(s) 2409, 2615, 3597, 4099
EcoRI GAATTC 2 cut(s) 1623, 3604
EcoRV GATATC 2 cut(s) 1498, 2275
EcoT14I CCWWGG 4 cut(s) 71, 222, 3025, 3862
EcoT38I GRGCYC 3 cut(s) 147, 2908, 3601
EgeI GGCGCC 1 cut(s) 4059
EheI GGCGCC 1 cut(s) 4059
ErhI CCWWGG 4 cut(s) 71, 222, 3025, 3862
Esp3I CGTCTC 1 cut(s) 109
FaqI GGGAC 4 cut(s) 893, 2954, 3896, 4085
FblI GTMKAC 1 cut(s) 2047
FriOI GRGCYC 3 cut(s) 147, 2908, 3601
FspBI CTAG 4 cut(s) 522, 816, 2493, 2810
GlaI GCGC 9 cut(s) 293, 908, 2098, 2138, 3828, 3897, 3924, 3951, 4059
GsuI CTGGAG 8 cut(s) 1965, 2277, 2894, 3497, 3525, 3576, 3738, 3786
HaeII RGCGCY 4 cut(s) 3899, 3926, 3953, 4061
HapII CCGG 7 cut(s) 22, 169, 624, 1802, 2703, 2716, 3620
HhaI GCGC 9 cut(s) 294, 909, 2099, 2139, 3829, 3898, 3925, 3952, 4060
Hin1I GRCGYC 1 cut(s) 4058
Hin6I GCGC 9 cut(s) 292, 907, 2097, 2137, 3827, 3896, 3923, 3950, 4058
HinP1I GCGC 9 cut(s) 292, 907, 2097, 2137, 3827, 3896, 3923, 3950, 4058
HincII GTYRAC 2 cut(s) 2539, 3207
HindII GTYRAC 2 cut(s) 2539, 3207
HindIII AAGCTT 2 cut(s) 605, 3488
HpaI GTTAAC 1 cut(s) 2539
HpaII CCGG 7 cut(s) 22, 169, 624, 1802, 2703, 2716, 3620
Hpy166II GTNNAC 9 cut(s) 271, 1061, 1117, 1799, 2048, 2539, 3207, 3229, 3701
Hpy8I GTNNAC 9 cut(s) 271, 1061, 1117, 1799, 2048, 2539, 3207, 3229, 3701
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 7 cut(s) 1353, 1633, 2305, 2927, 2968, 3301, 3678
HpyCH4III ACNGT 6 cut(s) 317, 1121, 2086, 2208, 3562, 3743
HpyCH4IV ACGT 2 cut(s) 102, 288
HpySE526I ACGT 2 cut(s) 102, 288
Hsp92I GRCGYC 1 cut(s) 4058
HspAI GCGC 9 cut(s) 292, 907, 2097, 2137, 3827, 3896, 3923, 3950, 4058
KasI GGCGCC 1 cut(s) 4057
KspAI GTTAAC 1 cut(s) 2539
LguI GCTCTTC 1 cut(s) 3186
MabI ACCWGGT 1 cut(s) 1149
MaeI CTAG 4 cut(s) 522, 816, 2493, 2810
MaeII ACGT 2 cut(s) 102, 288
MaeIII GTNAC 6 cut(s) 79, 311, 448, 1526, 2218, 2578
MfeI CAATTG 2 cut(s) 849, 3525
MflI RGATCY 6 cut(s) 524, 638, 1163, 1202, 1778, 3058
MhlI GDGCHC 8 cut(s) 147, 970, 1828, 2466, 2680, 2908, 3429, 3601
MlsI TGGCCA 2 cut(s) 1492, 2879
MluNI TGGCCA 2 cut(s) 1492, 2879
Mly113I GGCGCC 1 cut(s) 4058
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 7 cut(s) 86, 160, 171, 407, 1329, 1346, 2844
Mox20I TGGCCA 2 cut(s) 1492, 2879
MroXI GAANNNNTTC 3 cut(s) 243, 2028, 3534
MscI TGGCCA 2 cut(s) 1492, 2879
MslI CAYNNNNRTG 6 cut(s) 789, 952, 963, 2787, 3354, 3360
Msp20I TGGCCA 2 cut(s) 1492, 2879
MspA1I CMGCKG 9 cut(s) 326, 1214, 1673, 1679, 1703, 1712, 1985, 2712, 3275
MspCI CTTAAG 1 cut(s) 3113
MspI CCGG 7 cut(s) 22, 169, 624, 1802, 2703, 2716, 3620
MunI CAATTG 2 cut(s) 849, 3525
Mva1269I GAATGC 3 cut(s) 1252, 3266, 3333
NarI GGCGCC 1 cut(s) 4058
NciI CCSGG 2 cut(s) 2703, 3621
NcoI CCATGG 1 cut(s) 71
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 3 cut(s) 79, 448, 1526
NspI RCATGY 4 cut(s) 1088, 1394, 1581, 2453
OliI CACNNNNGTG 1 cut(s) 963
PacI TTAATTAA 1 cut(s) 4198
PaeI GCATGC 1 cut(s) 2453
PagI TCATGA 2 cut(s) 2785, 3160
PceI AGGCCT 2 cut(s) 4046, 4073
PciI ACATGT 2 cut(s) 1084, 1390
PciSI GCTCTTC 1 cut(s) 3186
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 84
PctI GAATGC 3 cut(s) 1252, 3266, 3333
PdmI GAANNNNTTC 3 cut(s) 243, 2028, 3534
PflMI CCANNNNNTGG 2 cut(s) 1011, 1328
PfoI TCCNGGA 1 cut(s) 3777
PinAI ACCGGT 1 cut(s) 1801
PleI GAGTC 1 cut(s) 212
PluTI GGCGCC 1 cut(s) 4061
PpsI GAGTC 1 cut(s) 212
PpuMI RGGWCCY 2 cut(s) 2615, 4099
PscI ACATGT 2 cut(s) 1084, 1390
Psp124BI GAGCTC 2 cut(s) 147, 2908
Psp5II RGGWCCY 2 cut(s) 2615, 4099
PspOMI GGGCCC 1 cut(s) 3597
PspPI GGNCC 9 cut(s) 1014, 1533, 2372, 2409, 2615, 3597, 3598, 3866, 4099
PspPPI RGGWCCY 2 cut(s) 2615, 4099
PstI CTGCAG 7 cut(s) 325, 1678, 1684, 1702, 1723, 3280, 3460
PstNI CAGNNNCTG 5 cut(s) 329, 1874, 2806, 3509, 3650
PsuI RGATCY 6 cut(s) 524, 638, 1163, 1202, 1778, 3058
PvuII CAGCTG 8 cut(s) 326, 1214, 1673, 1679, 1703, 1712, 1985, 3275
RsaI GTAC 6 cut(s) 471, 585, 762, 1420, 1731, 2319
RsaNI GTAC 6 cut(s) 470, 584, 761, 1419, 1730, 2318
RseI CAYNNNNRTG 6 cut(s) 789, 952, 963, 2787, 3354, 3360
SacI GAGCTC 2 cut(s) 147, 2908
SapI GCTCTTC 1 cut(s) 3186
Sau96I GGNCC 9 cut(s) 1014, 1533, 2372, 2409, 2615, 3597, 3598, 3866, 4099
ScaI AGTACT 1 cut(s) 585
SchI GAGTC 1 cut(s) 212
SduI GDGCHC 8 cut(s) 147, 970, 1828, 2466, 2680, 2908, 3429, 3601
SexAI ACCWGGT 1 cut(s) 1149
SfoI GGCGCC 1 cut(s) 4059
SgrAI CRCCGGYG 1 cut(s) 168
SinI GGWCC 4 cut(s) 1533, 2372, 2615, 4099
SmiMI CAYNNNNRTG 6 cut(s) 789, 952, 963, 2787, 3354, 3360
SmlI CTYRAG 3 cut(s) 741, 3113, 4080
SmoI CTYRAG 3 cut(s) 741, 3113, 4080
SphI GCATGC 1 cut(s) 2453
SseBI AGGCCT 2 cut(s) 4046, 4073
SspDI GGCGCC 1 cut(s) 4057
SspI AATATT 1 cut(s) 1768
SspMI CTAG 4 cut(s) 522, 816, 2493, 2810
SstI GAGCTC 2 cut(s) 147, 2908
StuI AGGCCT 2 cut(s) 4046, 4073
StyI CCWWGG 4 cut(s) 71, 222, 3025, 3862
TaaI ACNGT 6 cut(s) 317, 1121, 2086, 2208, 3562, 3743
TaiI ACGT 2 cut(s) 105, 291
TaqI TCGA 8 cut(s) 162, 1020, 1246, 1621, 1652, 1846, 2235, 2277
TatI WGTACW 2 cut(s) 583, 1729
TauI GCSGC 8 cut(s) 907, 3074, 3134, 3857, 3928, 3955, 4009, 4063
TscAI CASTG 7 cut(s) 241, 1192, 1558, 1801, 2896, 2918, 3636
TseFI GTSAC 3 cut(s) 79, 448, 1526
Tsp45I GTSAC 3 cut(s) 79, 448, 1526
TspDTI ATGAA 6 cut(s) 1428, 1638, 2828, 3177, 3338, 3720
TspGWI ACGGA 3 cut(s) 97, 1833, 3461
TspRI CASTG 7 cut(s) 241, 1192, 1558, 1801, 2896, 2918, 3636
Van91I CCANNNNNTGG 2 cut(s) 1011, 1328
Vha464I CTTAAG 1 cut(s) 3113
VpaK11BI GGWCC 4 cut(s) 1533, 2372, 2615, 4099
XapI RAATTY 7 cut(s) 512, 1190, 1623, 3038, 3531, 3604, 3784
XbaI TCTAGA 3 cut(s) 521, 815, 2492
XceI RCATGY 4 cut(s) 1088, 1394, 1581, 2453
XcmI CCANNNNNNNNNTGG 1 cut(s) 69
XmiI GTMKAC 1 cut(s) 2047
XmnI GAANNNNTTC 3 cut(s) 243, 2028, 3534
XspI CTAG 4 cut(s) 522, 816, 2493, 2810
ZrmI AGTACT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.