Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0017373

Physical Location & Seq

Genomic Coordinates Forward (+)

25157601 .. 25158370

Gene Structure 770 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1160800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 339 bp

ATGGATGATCAATCAGACGGCGGAGAAGGCAACGCCGGAAAAAACCCGAAGCTGCCGGTCCCTGGGAAGCGCAACATCCTCATCACAAGCGCCTTGCCCTATGTCAACAACATCCCTCGCCTTGGCACCATCAGTGGCTGTAAGTACATTGTGATGTACATATGTGGGACTGATGAGTATGGGACTGCAACTGAGACAAAAGCTAAGGACGAAAATTGTACTCCACAGCAAATTTGTGACAAGTTATATCATGAAATTCATAAACAGGTCTACCAGTGGTTCGATATAAGTTTTGATAAATTCGGAACCACGTCAACTCCTCAACATACTGAAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

tRNA-synt_1g tRNA_bind

Protein Analysis

113

Amino Acids

12.5

Weight (kDa)

5.5

Isoelectric Point (pI)

48.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	26 - 112	4.3e-19	tRNA synthetases class I (M)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017373)

Species	Orthologous Gene IDs
malus_domestica	MD10G1160800.v1.1
rosa_chinensis	RchiOBHm_Chr4g0414261 RchiOBHm_Chr4g0445731
rosa_laevigata	RLG00000005712
rosa_multiflora	Rmu_sc0003637.1_g000009 Rmu_sc0003637.1_g000010
rosa_roxburghii	Rroxscaffold_2G00121530
rosa_samantha	Rh4AG426200 Rh4CG453200 Rh4DG180400 Rh4DG181300 Rh4DG434400
rosa_wichuraiana	Rw4G036500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	125
AccI	GTMKAC	1 cut(s)	270
AciI	CCGC	1 cut(s)	21
AcsI	RAATTY	4 cut(s)	231, 255, 299, 333
AfaI	GTAC	3 cut(s)	146, 158, 220
AfiI	CCNNNNNNNGG	2 cut(s)	62, 122
AjiI	CACGTC	1 cut(s)	312
AjnI	CCWGG	1 cut(s)	61
AluBI	AGCT	2 cut(s)	52, 203
AluI	AGCT	2 cut(s)	52, 203
Alw26I	GTCTC	1 cut(s)	188
AlwNI	CAGNNNCTG	1 cut(s)	138
ApeKI	GCWGC	1 cut(s)	52
ApoI	RAATTY	4 cut(s)	231, 255, 299, 333
AspLEI	GCGC	2 cut(s)	72, 92
AspS9I	GGNCC	1 cut(s)	58
AvaII	GGWCC	1 cut(s)	58
BanI	GGYRCC	1 cut(s)	125
BbvI	GCAGC	1 cut(s)	39
BccI	CCATC	1 cut(s)	137
BceAI	ACGGC	1 cut(s)	34
BciT130I	CCWGG	1 cut(s)	63
BclI	TGATCA	1 cut(s)	7
BcoDI	GTCTC	1 cut(s)	188
BfoI	RGCGCY	1 cut(s)	93
BisI	GCNGC	1 cut(s)	53
BlsI	GCNGC	1 cut(s)	54
Bme1390I	CCNGG	1 cut(s)	63
Bme18I	GGWCC	1 cut(s)	58
BmgBI	CACGTC	1 cut(s)	312
BmgT120I	GGNCC	1 cut(s)	58
BmiI	GGNNCC	3 cut(s)	60, 127, 307
BmrFI	CCNGG	1 cut(s)	63
Bpu10I	CCTNAGC	1 cut(s)	204
BsaJI	CCNNGG	3 cut(s)	61, 62, 121
BsaXI	ACNNNNNCTCC	2 cut(s)	301, 331
Bsc4I	CCNNNNNNNGG	2 cut(s)	62, 122
Bse118I	RCCGGY	1 cut(s)	55
Bse1I	ACTGG	1 cut(s)	274
BseBI	CCWGG	1 cut(s)	63
BseDI	CCNNGG	3 cut(s)	61, 62, 121
BseGI	GGATG	3 cut(s)	10, 75, 111
BseLI	CCNNNNNNNGG	2 cut(s)	62, 122
BseMII	CTCAG	1 cut(s)	183
BseNI	ACTGG	1 cut(s)	274
BseRI	GAGGAG	1 cut(s)	309
BseXI	GCAGC	1 cut(s)	39
BshNI	GGYRCC	1 cut(s)	125
BsiSI	CCGG	2 cut(s)	36, 56
BslFI	GGGAC	3 cut(s)	44, 181, 196
BslI	CCNNNNNNNGG	2 cut(s)	62, 122
BsmAI	GTCTC	1 cut(s)	188
BsmFI	GGGAC	3 cut(s)	44, 181, 196
Bsp1407I	TGTACA	1 cut(s)	156
Bsp143I	GATC	1 cut(s)	7
BspACI	CCGC	1 cut(s)	21
BspCNI	CTCAG	1 cut(s)	184
BspHI	TCATGA	1 cut(s)	250
BspLI	GGNNCC	3 cut(s)	60, 127, 307
BspT107I	GGYRCC	1 cut(s)	125
BsrFI	RCCGGY	1 cut(s)	55
BsrGI	TGTACA	1 cut(s)	156
BsrI	ACTGG	1 cut(s)	274
BssAI	RCCGGY	1 cut(s)	55
BssECI	CCNNGG	3 cut(s)	61, 62, 121
BssMI	GATC	1 cut(s)	7
BssT1I	CCWWGG	1 cut(s)	121
Bst2UI	CCWGG	1 cut(s)	63
BstAUI	TGTACA	1 cut(s)	156
BstDEI	CTNAG	2 cut(s)	192, 204
BstF5I	GGATG	3 cut(s)	10, 75, 111
BstH2I	RGCGCY	1 cut(s)	93
BstHHI	GCGC	2 cut(s)	72, 92
BstKTI	GATC	1 cut(s)	10
BstMAI	GTCTC	1 cut(s)	188
BstMBI	GATC	1 cut(s)	7
BstMWI	GCNNNNNNNGC	1 cut(s)	27
BstNI	CCWGG	1 cut(s)	63
BstSCI	CCNGG	1 cut(s)	61
BstV1I	GCAGC	1 cut(s)	39
BtrI	CACGTC	1 cut(s)	312
BtsCI	GGATG	3 cut(s)	10, 75, 111
BtsIMutI	CAGTG	2 cut(s)	139, 281
CaiI	CAGNNNCTG	1 cut(s)	138
CciI	TCATGA	1 cut(s)	250
CfoI	GCGC	2 cut(s)	72, 92
Cfr10I	RCCGGY	1 cut(s)	55
Cfr13I	GGNCC	1 cut(s)	58
Csp6I	GTAC	3 cut(s)	145, 157, 219
CviAII	CATG	1 cut(s)	251
CviJI	RGCY	3 cut(s)	52, 138, 203
CviKI_1	RGCY	3 cut(s)	52, 138, 203
CviQI	GTAC	3 cut(s)	145, 157, 219
DdeI	CTNAG	2 cut(s)	192, 204
DpnI	GATC	1 cut(s)	9
DpnII	GATC	1 cut(s)	7
EciI	GGCGGA	1 cut(s)	36
Eco130I	CCWWGG	1 cut(s)	121
Eco47I	GGWCC	1 cut(s)	58
EcoRII	CCWGG	1 cut(s)	61
EcoT14I	CCWWGG	1 cut(s)	121
ErhI	CCWWGG	1 cut(s)	121
FaeI	CATG	1 cut(s)	254
FaiI	YATR	9 cut(s)	102, 161, 163, 180, 247, 252, 261, 287, 327
FaqI	GGGAC	3 cut(s)	44, 181, 196
FatI	CATG	1 cut(s)	250
FauNDI	CATATG	1 cut(s)	161
FbaI	TGATCA	1 cut(s)	7
FblI	GTMKAC	1 cut(s)	270
Fnu4HI	GCNGC	1 cut(s)	53
FokI	GGATG	3 cut(s)	17, 62, 98
Fsp4HI	GCNGC	1 cut(s)	53
GlaI	GCGC	2 cut(s)	71, 91
GluI	GCNGC	1 cut(s)	53
HaeII	RGCGCY	1 cut(s)	93
HapII	CCGG	2 cut(s)	36, 56
HhaI	GCGC	2 cut(s)	72, 92
Hin1II	CATG	1 cut(s)	254
Hin6I	GCGC	2 cut(s)	70, 90
HinP1I	GCGC	2 cut(s)	70, 90
HincII	GTYRAC	2 cut(s)	106, 315
HindII	GTYRAC	2 cut(s)	106, 315
HpaII	CCGG	2 cut(s)	36, 56
Hpy166II	GTNNAC	3 cut(s)	106, 271, 315
Hpy188I	TCNGA	2 cut(s)	16, 305
Hpy188III	TCNNGA	1 cut(s)	251
Hpy8I	GTNNAC	3 cut(s)	106, 271, 315
HpyAV	CCTTC	1 cut(s)	20
HpyCH4IV	ACGT	1 cut(s)	311
HpyCH4V	TGCA	1 cut(s)	188
HpyF10VI	GCNNNNNNNGC	1 cut(s)	27
HpyF3I	CTNAG	2 cut(s)	192, 204
HpySE526I	ACGT	1 cut(s)	311
Hsp92II	CATG	1 cut(s)	254
HspAI	GCGC	2 cut(s)	70, 90
Ksp22I	TGATCA	1 cut(s)	7
Kzo9I	GATC	1 cut(s)	7
LpnPI	CCDG	6 cut(s)	48, 49, 69, 75, 251, 287
Lsp1109I	GCAGC	1 cut(s)	39
MaeII	ACGT	1 cut(s)	311
MaeIII	GTNAC	1 cut(s)	236
MalI	GATC	1 cut(s)	9
MboI	GATC	1 cut(s)	7
MluCI	AATT	5 cut(s)	214, 231, 255, 299, 333
MnlI	CCTC	3 cut(s)	89, 126, 330
MslI	CAYNNNNRTG	1 cut(s)	152
MspI	CCGG	2 cut(s)	36, 56
MspR9I	CCNGG	1 cut(s)	63
MvaI	CCWGG	1 cut(s)	63
MwoI	GCNNNNNNNGC	1 cut(s)	27
NdeI	CATATG	1 cut(s)	161
NdeII	GATC	1 cut(s)	7
NlaIII	CATG	1 cut(s)	254
NlaIV	GGNNCC	3 cut(s)	60, 127, 307
NmuCI	GTSAC	1 cut(s)	236
PagI	TCATGA	1 cut(s)	250
PasI	CCCWGGG	1 cut(s)	62
PkrI	GCNGC	1 cut(s)	54
Psp6I	CCWGG	1 cut(s)	61
PspGI	CCWGG	1 cut(s)	61
PspN4I	GGNNCC	3 cut(s)	60, 127, 307
PspPI	GGNCC	1 cut(s)	58
PstNI	CAGNNNCTG	1 cut(s)	138
RsaI	GTAC	3 cut(s)	146, 158, 220
RsaNI	GTAC	3 cut(s)	145, 157, 219
RseI	CAYNNNNRTG	1 cut(s)	152
SatI	GCNGC	1 cut(s)	53
Sau3AI	GATC	1 cut(s)	7
Sau96I	GGNCC	1 cut(s)	58
ScrFI	CCNGG	1 cut(s)	63
SetI	ASST	4 cut(s)	54, 205, 270, 314
SinI	GGWCC	1 cut(s)	58
SmiMI	CAYNNNNRTG	1 cut(s)	152
Sse9I	AATT	5 cut(s)	214, 231, 255, 299, 333
SsiI	CCGC	1 cut(s)	21
StyD4I	CCNGG	1 cut(s)	61
StyI	CCWWGG	1 cut(s)	121
TaiI	ACGT	1 cut(s)	314
TaqI	TCGA	1 cut(s)	282
TasI	AATT	5 cut(s)	214, 231, 255, 299, 333
TatI	WGTACW	3 cut(s)	144, 156, 218
TscAI	CASTG	2 cut(s)	139, 281
TseFI	GTSAC	1 cut(s)	236
TseI	GCWGC	1 cut(s)	52
Tsp45I	GTSAC	1 cut(s)	236
TspDTI	ATGAA	2 cut(s)	248, 267
TspRI	CASTG	2 cut(s)	139, 281
VpaK11BI	GGWCC	1 cut(s)	58
XapI	RAATTY	4 cut(s)	231, 255, 299, 333
XmiI	GTMKAC	1 cut(s)	270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.