MD10G1180100.v1.1

Piriformospora indica-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011930
Physical Location & Seq
Forward (+)
27359345 .. 27360746
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1180100.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGCTCTGTTTCATCCTTTTTCCAAACTTACCCTCCTCCTCCTCCTCCTCCTCACAACCCTCCTGTCCATAGCCGTCATTTCACAACCCCTCCTCACCCCCGCCGAGCAAGACGCCGTCTACACAGTTCTTGGCTCCGTCAACCCCGACATCTCCTGGCGCTCCCTCTTCCCCGACGACCTCTGCCTCTCCGCCCCTCACGGTGTCGTCTGCGACTTCTTCTACGACAACGTTTCGTCCCCCGAACCGGTGGCGCACGTGACCGAGATGAATTTCGGGTACGTTTCGGACTACACCCCGAACCCGCCGTGCTCCGCCAACGCCACCTTCAGCCCGCTCCTCTTCACCTCCTTCAAGTACCTCCGCAAGCTCTTCTTCTACCGCTGCTTCACCGGAACGCGCGTTTCGTTCCCCGAAATCCCCAAGAGTTTCGGGTCCGGTTTGGAGGAGCTCGTTTTCATCGACAACCCGTCTCTGGTGGGCTCCCTCAGTGTAATAATCCGTAATTTCACCGACTTGAGAAGGGTGGTTCTGACCGGAAATGGGGTTTACGGGAACATCCCGGACGGCGTCGCCGACCTGGTTAACCTGGAGGAGCTGACTCTGTCTCGGAACCAACTCGGCGGCGAAATCCCCCTGATGAGCTTCTCGAAGCTGAAGAAGCTGAAGGTTCTTGATCTGGGTTACAATTATTTTTCCGGAACTGTCCCTGAATCGGTGGGAAATCTCTCGGAGCTGGTGAAGCTGGACCTGAGATCCAATGGGTTTAATGGTAAAATCCCAGAGAGCTTGAAGAACTTGAAAACTTTGGAGCTTCTGGACCTGAGCCGCAACAATTTCACCAACTATGGGGTTCCTCTGTTTCTGGGTGATATGCCAAGGCTGAAGCAATTAGATTTGAGCGGGAACTTGCTGGGAGGGCAGATTCCGAAAATTTGGGACAAGCTTGGGGGTATTCTAGGAATTGGGTTTTCAGAAATGGGGCTGGTTGGTGAAATTCCAGCTTCAATGGGGGTGCATTTGAAGAATCTAAGGTATTTAGGGCTTGATAACAACAAGCTTGAAGGGACGGTGCCTGAGGAGTTAGGGCTCTTGGAATCTGTGAGCGTGATTAATTTGCAGAACAATAACCTCAGTGGCAGAGTTTCATTTGGTGGTAGTAAATTTTCAGCAAAATTTGGGCAAAATTTGAAACTGGCTGGAAACCCTAATCTTTGTGCCGATGATAAGGCTTTGATCCTCTCCTCCAAGACCCAGCTGAAGGTCTGCAAGAAAACCCAGAAACCCAATTCTGCCCCTCTAACTCTGGACTCTTCAGTGCAAGTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

444

Amino Acids

48.3

Weight (kDa)

6.16

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 196 - 255 1.1e-08 Leucine rich repeat
LRR_4 PF12799 196 - 230 1e-05 Leucine Rich repeats (2 copies)
LRR_14 PF23598 202 - 300 3.8e-07 Leucine-rich repeat region
LRR_8 PF13855 245 - 305 1.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28560
fragaria_vesca FvH4_2g21070
malus_domestica MD10G1180100.v1.1
prunus_persica Prupe.8G220100_v2.0.a1
pyrus_communis pycom05g18060 pycom10g15600
rosa_chinensis RchiOBHm_Chr6g0287531
rosa_laevigata RLG00000012487
rosa_multiflora Rmu_sc0004974.1_g000008 Rmu_sc0007029.1_g000010
rosa_roxburghii Rroxscaffold_7G00180930 Rroxscaffold_7G00181400
rosa_rugosa Rorug06G0188700
rosa_samantha Rh6AG300200 Rh6BG305500 Rh6CG304000 Rh6CG314000 Rh6DG297400
rosa_wichuraiana Rw6G025880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1072
AccBSI CCGCTC 2 cut(s) 337, 903
AccI GTMKAC 1 cut(s) 120
AccII CGCG 2 cut(s) 400, 402
AccIII TCCGGA 1 cut(s) 698
AclI AACGTT 1 cut(s) 231
AclWI GGATC 2 cut(s) 750, 1231
AcsI RAATTY 6 cut(s) 271, 933, 996, 1163, 1175, 1186
AcuI CTGAAG 6 cut(s) 313, 677, 686, 905, 1280, 1299
AcvI CACGTG 1 cut(s) 259
AcyI GRCGYC 2 cut(s) 114, 570
AfaI GTAC 2 cut(s) 281, 359
AfiI CCNNNNNNNGG 6 cut(s) 247, 475, 543, 715, 849, 1261
AgeI ACCGGT 1 cut(s) 247
AgsI TTSAA 7 cut(s) 355, 793, 802, 1008, 1024, 1064, 1192
AhdI GACNNNNNGTC 1 cut(s) 469
AjnI CCWGG 3 cut(s) 155, 579, 588
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
Alw21I GWGCWC 2 cut(s) 314, 453
Alw26I GTCTC 2 cut(s) 477, 612
AlwI GGATC 2 cut(s) 750, 1231
Aor13HI TCCGGA 1 cut(s) 698
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 6 cut(s) 271, 933, 996, 1163, 1175, 1186
AseI ATTAAT 1 cut(s) 1113
AsiGI ACCGGT 1 cut(s) 247
AspLEI GCGC 3 cut(s) 162, 256, 402
AspS9I GGNCC 3 cut(s) 435, 748, 820
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 8 cut(s) 88, 337, 382, 502, 751, 832, 881, 1004
AvaII GGWCC 3 cut(s) 435, 748, 820
AxyI CCTNAGG 1 cut(s) 1077
BanI GGYRCC 1 cut(s) 1072
BanII GRGCYC 3 cut(s) 453, 485, 1092
BbrPI CACGTG 1 cut(s) 259
Bbv12I GWGCWC 2 cut(s) 314, 453
BbvI GCAGC 1 cut(s) 371
BceAI ACGGC 4 cut(s) 59, 101, 292, 583
BciT130I CCWGG 3 cut(s) 157, 581, 590
BcnI CCSGG 1 cut(s) 563
BcoDI GTCTC 2 cut(s) 477, 612
BfaI CTAG 1 cut(s) 959
BfoI RGCGCY 1 cut(s) 163
BisI GCNGC 3 cut(s) 385, 625, 829
BlsI GCNGC 3 cut(s) 386, 626, 830
Bme1390I CCNGG 4 cut(s) 157, 563, 581, 590
Bme18I GGWCC 3 cut(s) 435, 748, 820
BmeRI GACNNNNNGTC 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 435, 748, 820
BmiI GGNNCC 6 cut(s) 136, 436, 484, 614, 855, 1074
BmrFI CCNGG 4 cut(s) 157, 563, 581, 590
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 824
BpuEI CTTGAG 1 cut(s) 538
BpuMI CCSGG 1 cut(s) 563
BsaAI YACGTR 1 cut(s) 259
BsaHI GRCGYC 2 cut(s) 114, 570
BsaJI CCNNGG 1 cut(s) 878
BsaWI WCCGGW 5 cut(s) 247, 392, 437, 536, 698
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 75, 105
Bsc4I CCNNNNNNNGG 6 cut(s) 247, 475, 543, 715, 849, 1261
Bse118I RCCGGY 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 1200
Bse21I CCTNAGG 1 cut(s) 1077
BseAI TCCGGA 1 cut(s) 698
BseBI CCWGG 3 cut(s) 157, 581, 590
BseDI CCNNGG 1 cut(s) 878
BseGI GGATG 2 cut(s) 13, 558
BseLI CCNNNNNNNGG 6 cut(s) 247, 475, 543, 715, 849, 1261
BseMII CTCAG 5 cut(s) 502, 743, 815, 1068, 1147
BseNI ACTGG 1 cut(s) 1200
BseXI GCAGC 1 cut(s) 371
BseYI CCCAGC 2 cut(s) 913, 1254
Bsh1236I CGCG 2 cut(s) 400, 402
BshNI GGYRCC 1 cut(s) 1072
BshTI ACCGGT 1 cut(s) 247
BsiHKAI GWGCWC 2 cut(s) 314, 453
BsiSI CCGG 6 cut(s) 248, 393, 438, 537, 563, 699
BslFI GGGAC 4 cut(s) 223, 692, 953, 1081
BslI CCNNNNNNNGG 6 cut(s) 247, 475, 543, 715, 849, 1261
BsmAI GTCTC 2 cut(s) 477, 612
BsmBI CGTCTC 1 cut(s) 477
BsmFI GGGAC 4 cut(s) 223, 692, 953, 1081
Bsp1286I GDGCHC 4 cut(s) 314, 453, 485, 1092
Bsp13I TCCGGA 1 cut(s) 698
Bsp143I GATC 3 cut(s) 676, 755, 1236
BspCNI CTCAG 5 cut(s) 501, 744, 816, 1069, 1146
BspEI TCCGGA 1 cut(s) 698
BspFNI CGCG 2 cut(s) 400, 402
BspLI GGNNCC 6 cut(s) 136, 436, 484, 614, 855, 1074
BspPI GGATC 2 cut(s) 750, 1231
BspQI GCTCTTC 1 cut(s) 377
BspT107I GGYRCC 1 cut(s) 1072
BsrBI CCGCTC 2 cut(s) 337, 903
BsrFI RCCGGY 1 cut(s) 247
BsrI ACTGG 1 cut(s) 1200
BssAI RCCGGY 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 878
BssMI GATC 3 cut(s) 676, 755, 1236
BssNI GRCGYC 2 cut(s) 114, 570
BssT1I CCWWGG 1 cut(s) 878
Bst2UI CCWGG 3 cut(s) 157, 581, 590
Bst4CI ACNGT 4 cut(s) 127, 203, 706, 1072
Bst6I CTCTTC 4 cut(s) 173, 347, 377, 1318
BstACI GRCGYC 2 cut(s) 114, 570
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 335, 368
BstDEI CTNAG 6 cut(s) 488, 752, 824, 1031, 1077, 1133
BstF5I GGATG 2 cut(s) 13, 558
BstFNI CGCG 2 cut(s) 400, 402
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 3 cut(s) 162, 256, 402
BstKTI GATC 3 cut(s) 679, 758, 1239
BstMAI GTCTC 2 cut(s) 477, 612
BstMBI GATC 3 cut(s) 676, 755, 1236
BstMWI GCNNNNNNNGC 3 cut(s) 661, 742, 919
BstNI CCWGG 3 cut(s) 157, 581, 590
BstSCI CCNGG 4 cut(s) 155, 561, 579, 588
BstUI CGCG 2 cut(s) 400, 402
BstV1I GCAGC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
Bsu36I CCTNAGG 1 cut(s) 1077
BtsCI GGATG 2 cut(s) 13, 558
BtsIMutI CAGTG 3 cut(s) 496, 1141, 1323
Cac8I GCNNGC 2 cut(s) 335, 368
CfoI GCGC 3 cut(s) 162, 256, 402
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 435, 748, 820
CseI GACGC 2 cut(s) 122, 559
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 2 cut(s) 280, 358
CspAI ACCGGT 1 cut(s) 247
CviQI GTAC 2 cut(s) 280, 358
DdeI CTNAG 6 cut(s) 488, 752, 824, 1031, 1077, 1133
DpnI GATC 3 cut(s) 678, 757, 1238
DpnII GATC 3 cut(s) 676, 755, 1236
DriI GACNNNNNGTC 1 cut(s) 469
Eam1104I CTCTTC 4 cut(s) 173, 347, 377, 1318
Eam1105I GACNNNNNGTC 1 cut(s) 469
EarI CTCTTC 4 cut(s) 173, 347, 377, 1318
EciI GGCGGA 2 cut(s) 181, 304
Ecl136II GAGCTC 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 878
Eco24I GRGCYC 3 cut(s) 453, 485, 1092
Eco47I GGWCC 3 cut(s) 435, 748, 820
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 6 cut(s) 313, 677, 686, 905, 1280, 1299
Eco72I CACGTG 1 cut(s) 259
Eco81I CCTNAGG 1 cut(s) 1077
EcoICRI GAGCTC 1 cut(s) 451
EcoRII CCWGG 3 cut(s) 155, 579, 588
EcoT14I CCWWGG 1 cut(s) 878
EcoT38I GRGCYC 3 cut(s) 453, 485, 1092
ErhI CCWWGG 1 cut(s) 878
Esp3I CGTCTC 1 cut(s) 477
FaiI YATR 3 cut(s) 71, 849, 875
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 4 cut(s) 223, 692, 953, 1081
FauI CCCGC 4 cut(s) 109, 312, 342, 896
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 3 cut(s) 385, 625, 829
FokI GGATG 1 cut(s) 545
FriOI GRGCYC 3 cut(s) 453, 485, 1092
Fsp4HI GCNGC 3 cut(s) 385, 625, 829
FspBI CTAG 1 cut(s) 959
GlaI GCGC 3 cut(s) 161, 255, 401
GluI GCNGC 3 cut(s) 385, 625, 829
GsaI CCCAGC 2 cut(s) 917, 1258
GsuI CTGGAG 1 cut(s) 611
HaeII RGCGCY 1 cut(s) 163
HapII CCGG 6 cut(s) 248, 393, 438, 537, 563, 699
HgaI GACGC 2 cut(s) 122, 559
HhaI GCGC 3 cut(s) 162, 256, 402
Hin1I GRCGYC 2 cut(s) 114, 570
Hin6I GCGC 3 cut(s) 160, 254, 400
HinP1I GCGC 3 cut(s) 160, 254, 400
HincII GTYRAC 2 cut(s) 142, 586
HindII GTYRAC 2 cut(s) 142, 586
HindIII AAGCTT 2 cut(s) 944, 1058
HinfI GANTC 6 cut(s) 601, 713, 925, 1027, 1097, 1310
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 6 cut(s) 248, 393, 438, 537, 563, 699
HphI GGTGA 8 cut(s) 88, 337, 382, 502, 751, 832, 881, 1004
Hpy166II GTNNAC 4 cut(s) 121, 142, 550, 586
Hpy188I TCNGA 6 cut(s) 289, 534, 612, 733, 930, 976
Hpy188III TCNNGA 5 cut(s) 649, 674, 699, 818, 1307
Hpy8I GTNNAC 4 cut(s) 121, 142, 550, 586
Hpy99I CGWCG 2 cut(s) 179, 575
HpyAV CCTTC 6 cut(s) 337, 361, 516, 661, 1058, 1255
HpyCH4III ACNGT 4 cut(s) 127, 203, 706, 1072
HpyCH4IV ACGT 3 cut(s) 231, 258, 282
HpyCH4V TGCA 4 cut(s) 1018, 1120, 1269, 1321
HpyF10VI GCNNNNNNNGC 3 cut(s) 661, 742, 919
HpyF3I CTNAG 6 cut(s) 488, 752, 824, 1031, 1077, 1133
HpySE526I ACGT 3 cut(s) 231, 258, 282
Hsp92I GRCGYC 2 cut(s) 114, 570
HspAI GCGC 3 cut(s) 160, 254, 400
Kpn2I TCCGGA 1 cut(s) 698
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 3 cut(s) 676, 755, 1236
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 9 cut(s) 140, 167, 317, 342, 448, 488, 595, 733, 811
Lsp1109I GCAGC 1 cut(s) 371
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 1 cut(s) 959
MaeII ACGT 3 cut(s) 231, 258, 282
MaeIII GTNAC 2 cut(s) 259, 683
MalI GATC 3 cut(s) 678, 757, 1238
MbiI CCGCTC 2 cut(s) 337, 903
MboI GATC 3 cut(s) 676, 755, 1236
MboII GAAGA 9 cut(s) 160, 211, 334, 364, 367, 670, 805, 1036, 1305
MflI RGATCY 1 cut(s) 755
MhlI GDGCHC 4 cut(s) 314, 453, 485, 1092
MlyI GAGTC 2 cut(s) 595, 1304
MroI TCCGGA 1 cut(s) 698
MseI TTAA 4 cut(s) 585, 768, 1113, 1330
MspA1I CMGCKG 2 cut(s) 384, 1258
MspI CCGG 6 cut(s) 248, 393, 438, 537, 563, 699
MspR9I CCNGG 4 cut(s) 157, 563, 581, 590
MvaI CCWGG 3 cut(s) 157, 581, 590
MvnI CGCG 2 cut(s) 400, 402
MwoI GCNNNNNNNGC 3 cut(s) 661, 742, 919
NciI CCSGG 1 cut(s) 563
NdeII GATC 3 cut(s) 676, 755, 1236
NlaIV GGNNCC 6 cut(s) 136, 436, 484, 614, 855, 1074
NmeAIII GCCGAG 2 cut(s) 130, 600
NmuCI GTSAC 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 377
PcsI WCGNNNNNNNCGW 3 cut(s) 404, 459, 573
PfeI GAWTC 4 cut(s) 713, 925, 1027, 1097
PflFI GACNNNGTC 3 cut(s) 116, 569, 604
PfoI TCCNGGA 1 cut(s) 561
PinAI ACCGGT 1 cut(s) 247
PkrI GCNGC 3 cut(s) 386, 626, 830
PleI GAGTC 2 cut(s) 595, 1304
PmaCI CACGTG 1 cut(s) 259
PmlI CACGTG 1 cut(s) 259
PpsI GAGTC 2 cut(s) 595, 1304
Ppu21I YACGTR 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 1113
Psp124BI GAGCTC 1 cut(s) 453
Psp1406I AACGTT 1 cut(s) 231
Psp6I CCWGG 3 cut(s) 155, 579, 588
PspCI CACGTG 1 cut(s) 259
PspFI CCCAGC 2 cut(s) 913, 1254
PspGI CCWGG 3 cut(s) 155, 579, 588
PspN4I GGNNCC 6 cut(s) 136, 436, 484, 614, 855, 1074
PspPI GGNCC 3 cut(s) 435, 748, 820
PsuI RGATCY 1 cut(s) 755
PsyI GACNNNGTC 3 cut(s) 116, 569, 604
PvuII CAGCTG 1 cut(s) 1258
RsaI GTAC 2 cut(s) 281, 359
RsaNI GTAC 2 cut(s) 280, 358
SacI GAGCTC 1 cut(s) 453
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 4 cut(s) 585, 768, 1113, 1330
SatI GCNGC 3 cut(s) 385, 625, 829
Sau3AI GATC 3 cut(s) 676, 755, 1236
Sau96I GGNCC 3 cut(s) 435, 748, 820
SchI GAGTC 2 cut(s) 595, 1304
ScrFI CCNGG 4 cut(s) 157, 563, 581, 590
SduI GDGCHC 4 cut(s) 314, 453, 485, 1092
SexAI ACCWGGT 1 cut(s) 579
SinI GGWCC 3 cut(s) 435, 748, 820
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
SspMI CTAG 1 cut(s) 959
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 4 cut(s) 155, 561, 579, 588
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 4 cut(s) 127, 203, 706, 1072
TaiI ACGT 3 cut(s) 234, 261, 285
TaqI TCGA 2 cut(s) 462, 650
TaqII GACCGA 1 cut(s) 278
TauI GCSGC 2 cut(s) 627, 831
TfiI GAWTC 4 cut(s) 713, 925, 1027, 1097
Tru1I TTAA 4 cut(s) 585, 768, 1113, 1330
Tru9I TTAA 4 cut(s) 585, 768, 1113, 1330
TscAI CASTG 3 cut(s) 496, 1141, 1323
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 1 cut(s) 384
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 3 cut(s) 284, 448, 1137
TspGWI ACGGA 2 cut(s) 127, 491
TspRI CASTG 3 cut(s) 496, 1141, 1323
Tth111I GACNNNGTC 3 cut(s) 116, 569, 604
VpaK11BI GGWCC 3 cut(s) 435, 748, 820
VspI ATTAAT 1 cut(s) 1113
XapI RAATTY 6 cut(s) 271, 933, 996, 1163, 1175, 1186
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 1 cut(s) 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.