Rh6BG305500

Piriformospora indica-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011930
Physical Location & Seq
Forward (+)
53522994 .. 53524851
1858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG305500.1

Sequence Viewer

Length: 1446 bp
ATGGCTCAGCTCCACCCAATTCCCACATTCACCCTCCTCTTCCTCACAACCCTCCTGTCCTCTTTCGTCATTTCCCACTCCCAGCCCCTCCTCAGCCCCGCCGAGCAGGCCTCCGTCTACCGCGTCCTCGACTCCCTCAACCCCCACGTCCCCTGGCGCACCCTCTTCCCCGACGACCTCTGCTCCTACGGCCCCCACGGCGTCGTCTGCGACTTCTTCGCCAACGACACTGATCCCAGCGTCGTAACCGTCCACGTCTCCGAGCTCAGCTTCGGGTACGTCTCCGACTACACCCCCAACCCGCCCTGCTCCTCCACCGCCGTTCTCAGCCCGCTCCTCTTCTCCGCCTTCCCTTACCTCCGCAAGCTCTTCTTCTACCGCTGCTTCAACGAAACGACAGTCGTTTCGGTCCCTGATATTCCGGCGAGTTTCGGGTCGGGTCTGGAGGAGCTCGTCTTTATAGACAACCCCTCTCTTGTTTCCCCGCTCAGTGGCATTCTCCGTAATTTCACCAGCTTAAGAAGGGCAGTTCTGACCGGGAACGGGTTTTACGGCAACGTACCGGATTCCGTTGCCGAGATGGTCAACCTGGACGAGTTGACTCTCTCCGGAAACCAACTCGGTGGAGAGATTCCGGTGAGTTTTGAAAAACTGAAGAAGCTGAAGGTTCTCGACCTGGGCGGGAACTCCTTCGCCGGAAAAGTGCCGGACTCGGTGGGGAGCCTCTCGGAGCTGTTGAAGCTTGACCTGAGCTCCAATGGGTTTTCCGGCGAAATCCCGGAGAGCCTCAAAAACTTGCGGAGGCTGGAGCTATTGGACCTGAGCTACAACAGGTTCAACAACTCCGGCGTGCCTCTGTTCTTGTCGGAGATGACACAGCTGAGAACGGTGTCTCTGAGTGGGAATATGCTGGGAGGGCAGATTCCGGAGATATGGAGCAAGCTCGGGGGCATTTTGGGAATTGGGCTTTCTGGGATGGGACTGGTTGGGAAAATCCCAGCTTCAATGGGGGTGTATTTGAGAAATGTGAGGTACCTTGGGCTTGACAATAACAAACTGGAAGGGGCAGTGCCTCAGGAGTTTGGTCTTTTGGAATCTGTGAATGAGATTAATTTAGAGAACAATAGCTTAAGTGGGAGGGTCTCATTTTCTTCAGCTAAATTTGGGCACAAGATGAAGTTGTATGGAAACCCACAGCTCTGTGTTGATGATAAGGGTTTAAGGTTTGCAAAGGGTTTGGGGTTTGAGGAGTTGAAGGTGTGCAGCAAACAGCAGAAACCCAATTCTGCCCATTTCACAATGGATTCTTCTTCTTCTTCTTCAGCAGTGCAGGTTGTTGTTCCATCCATGGTTATGCTTTTGCACATTTACCCAAAATTTACAGAGATTCTGTCTGATAGTGAAAGAATGGGAAATGTATCTGAAATTCTGAAGTGGGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

481

Amino Acids

52.4

Weight (kDa)

5.58

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 189 - 299 2.5e-09 Leucine-rich repeat region
LRR_8 PF13855 196 - 254 7.8e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28560
fragaria_vesca FvH4_2g21070
malus_domestica MD10G1180100.v1.1
prunus_persica Prupe.8G220100_v2.0.a1
pyrus_communis pycom05g18060 pycom10g15600
rosa_chinensis RchiOBHm_Chr6g0287531
rosa_laevigata RLG00000012487
rosa_multiflora Rmu_sc0004974.1_g000008 Rmu_sc0007029.1_g000010
rosa_roxburghii Rroxscaffold_7G00180930 Rroxscaffold_7G00181400
rosa_rugosa Rorug06G0188700
rosa_samantha Rh6AG300200 Rh6BG305500 Rh6CG304000 Rh6CG314000 Rh6DG297400
rosa_wichuraiana Rw6G025880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1321
Acc65I GGTACC 1 cut(s) 1032
AccB1I GGYRCC 1 cut(s) 1032
AccBSI CCGCTC 2 cut(s) 334, 487
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 123
AccIII TCCGGA 2 cut(s) 608, 925
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 3 cut(s) 1160, 1376, 1425
AcuI CTGAAG 4 cut(s) 674, 683, 1137, 1305
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 3 cut(s) 278, 561, 1034
AfiI CCNNNNNNNGG 2 cut(s) 491, 543
AflII CTTAAG 2 cut(s) 517, 1129
AgsI TTSAA 6 cut(s) 388, 647, 739, 838, 1005, 1255
AjiI CACGTC 2 cut(s) 148, 256
AjnI CCWGG 3 cut(s) 152, 588, 675
Alw21I GWGCWC 3 cut(s) 267, 453, 755
Alw26I GTCTC 4 cut(s) 262, 286, 897, 1147
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 944
Aor13HI TCCGGA 2 cut(s) 608, 925
AoxI GGCC 2 cut(s) 108, 190
ApeKI GCWGC 2 cut(s) 381, 1263
ApoI RAATTY 3 cut(s) 1160, 1376, 1425
ArsI GACNNNNNNTTYG 4 cut(s) 384, 388, 416, 420
AseI ATTAAT 1 cut(s) 1110
Asp700I GAANNNNTTC 1 cut(s) 689
Asp718I GGTACC 1 cut(s) 1032
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 3 cut(s) 191, 409, 817
AsuC2I CCSGG 2 cut(s) 538, 779
AsuHPI GGTGA 3 cut(s) 22, 502, 649
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 2 cut(s) 409, 817
AxyI CCTNAGG 1 cut(s) 1074
BaeGI GKGCMC 1 cut(s) 1170
BanI GGYRCC 1 cut(s) 1032
BanII GRGCYC 3 cut(s) 267, 453, 755
Bbv12I GWGCWC 3 cut(s) 267, 453, 755
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 368, 1275
BccI CCATC 3 cut(s) 574, 970, 1351
BceAI ACGGC 4 cut(s) 205, 214, 305, 568
BciT130I CCWGG 3 cut(s) 154, 590, 677
BcnI CCSGG 2 cut(s) 538, 779
BcoDI GTCTC 4 cut(s) 262, 286, 897, 1147
BfrI CTTAAG 2 cut(s) 517, 1129
BfuAI ACCTGC 1 cut(s) 1321
BglI GCCNNNNNGGC 2 cut(s) 107, 198
BisI GCNGC 2 cut(s) 382, 1264
BlpI GCTNAGC 2 cut(s) 6, 266
BlsI GCNGC 2 cut(s) 383, 1265
Bme1390I CCNGG 5 cut(s) 154, 538, 590, 677, 779
Bme18I GGWCC 2 cut(s) 409, 817
BmeT110I CYCGRG 1 cut(s) 944
BmgBI CACGTC 2 cut(s) 148, 256
BmgT120I GGNCC 3 cut(s) 191, 409, 817
BmiI GGNNCC 4 cut(s) 193, 411, 722, 1034
BmrFI CCNGG 5 cut(s) 154, 538, 590, 677, 779
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 2 cut(s) 464, 827
Bpu10I CCTNAGC 3 cut(s) 92, 749, 821
Bpu1102I GCTNAGC 2 cut(s) 6, 266
BpuMI CCSGG 2 cut(s) 538, 779
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 1147
BsaJI CCNNGG 5 cut(s) 152, 196, 676, 1036, 1347
BsaWI WCCGGW 4 cut(s) 562, 608, 634, 925
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 737, 767
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 543
Bse1I ACTGG 2 cut(s) 987, 1062
Bse21I CCTNAGG 1 cut(s) 1074
BseAI TCCGGA 2 cut(s) 608, 925
BseBI CCWGG 3 cut(s) 154, 590, 677
BseDI CCNNGG 5 cut(s) 152, 196, 676, 1036, 1347
BseGI GGATG 2 cut(s) 981, 1343
BseLI CCNNNNNNNGG 2 cut(s) 491, 543
BseNI ACTGG 2 cut(s) 987, 1062
BseRI GAGGAG 6 cut(s) 26, 80, 301, 326, 461, 1262
BseSI GKGCMC 1 cut(s) 1170
BseXI GCAGC 2 cut(s) 368, 1275
BseYI CCCAGC 4 cut(s) 81, 236, 910, 997
BsgI GTGCAG 2 cut(s) 1282, 1349
Bsh1236I CGCG 1 cut(s) 123
BshFI GGCC 2 cut(s) 110, 192
BshNI GGYRCC 1 cut(s) 1032
BsiHKAI GWGCWC 3 cut(s) 267, 453, 755
BsiHKCI CYCGRG 1 cut(s) 944
BslFI GGGAC 3 cut(s) 134, 395, 993
BslI CCNNNNNNNGG 2 cut(s) 491, 543
BsmAI GTCTC 4 cut(s) 262, 286, 897, 1147
BsmBI CGTCTC 2 cut(s) 262, 286
BsmFI GGGAC 3 cut(s) 134, 395, 993
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 2 cut(s) 110, 192
Bso31I GGTCTC 1 cut(s) 1147
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 4 cut(s) 267, 453, 755, 1170
Bsp13I TCCGGA 2 cut(s) 608, 925
Bsp143I GATC 1 cut(s) 232
Bsp1720I GCTNAGC 2 cut(s) 6, 266
Bsp19I CCATGG 1 cut(s) 1347
BspANI GGCC 2 cut(s) 110, 192
BspEI TCCGGA 2 cut(s) 608, 925
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 4 cut(s) 193, 411, 722, 1034
BspMI ACCTGC 1 cut(s) 1321
BspPI GGATC 1 cut(s) 227
BspQI GCTCTTC 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 1032
BspTI CTTAAG 2 cut(s) 517, 1129
BspTNI GGTCTC 1 cut(s) 1147
BsrBI CCGCTC 2 cut(s) 334, 487
BsrI ACTGG 2 cut(s) 987, 1062
BssECI CCNNGG 5 cut(s) 152, 196, 676, 1036, 1347
BssMI GATC 1 cut(s) 232
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 1036, 1347
Bst2UI CCWGG 3 cut(s) 154, 590, 677
Bst4CI ACNGT 3 cut(s) 250, 400, 889
Bst6I CTCTTC 4 cut(s) 44, 170, 344, 374
BstACI GRCGYC 1 cut(s) 201
BstAFI CTTAAG 2 cut(s) 517, 1129
BstC8I GCNNGC 5 cut(s) 108, 332, 365, 851, 941
BstDSI CCRYGG 2 cut(s) 196, 1347
BstF5I GGATG 2 cut(s) 981, 1343
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 1 cut(s) 235
BstMAI GTCTC 4 cut(s) 262, 286, 897, 1147
BstMBI GATC 1 cut(s) 232
BstMWI GCNNNNNNNGC 6 cut(s) 107, 189, 198, 207, 739, 916
BstNI CCWGG 3 cut(s) 154, 590, 677
BstSCI CCNGG 5 cut(s) 152, 536, 588, 675, 777
BstSLI GKGCMC 1 cut(s) 1170
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 368, 1275
BstXI CCANNNNNNTGG 1 cut(s) 623
Bsu36I CCTNAGG 1 cut(s) 1074
BsuRI GGCC 2 cut(s) 110, 192
BtgI CCRYGG 2 cut(s) 196, 1347
BtrI CACGTC 2 cut(s) 148, 256
BtsCI GGATG 2 cut(s) 981, 1343
BtsI GCAGTG 2 cut(s) 1074, 1332
BtsIMutI CAGTG 4 cut(s) 228, 496, 1074, 1332
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 5 cut(s) 108, 332, 365, 851, 941
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 3 cut(s) 191, 409, 817
CseI GACGC 3 cut(s) 112, 190, 229
Csp6I GTAC 3 cut(s) 277, 560, 1033
CviAII CATG 1 cut(s) 1348
CviQI GTAC 3 cut(s) 277, 560, 1033
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
Eam1104I CTCTTC 4 cut(s) 44, 170, 344, 374
EarI CTCTTC 4 cut(s) 44, 170, 344, 374
EciI GGCGGA 1 cut(s) 334
Ecl136II GAGCTC 3 cut(s) 265, 451, 753
Eco130I CCWWGG 2 cut(s) 1036, 1347
Eco147I AGGCCT 1 cut(s) 110
Eco24I GRGCYC 3 cut(s) 267, 453, 755
Eco31I GGTCTC 1 cut(s) 1147
Eco47I GGWCC 2 cut(s) 409, 817
Eco53kI GAGCTC 3 cut(s) 265, 451, 753
Eco57I CTGAAG 4 cut(s) 674, 683, 1137, 1305
Eco81I CCTNAGG 1 cut(s) 1074
Eco88I CYCGRG 1 cut(s) 944
EcoICRI GAGCTC 3 cut(s) 265, 451, 753
EcoRII CCWGG 3 cut(s) 152, 588, 675
EcoT14I CCWWGG 2 cut(s) 1036, 1347
EcoT38I GRGCYC 3 cut(s) 267, 453, 755
ErhI CCWWGG 2 cut(s) 1036, 1347
Esp3I CGTCTC 2 cut(s) 262, 286
FaeI CATG 1 cut(s) 1351
FaiI YATR 6 cut(s) 461, 908, 934, 1185, 1349, 1355
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 3 cut(s) 134, 395, 993
FatI CATG 1 cut(s) 1347
FauI CCCGC 5 cut(s) 106, 309, 339, 492, 674
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 382, 1264
FokI GGATG 2 cut(s) 988, 1330
FriOI GRGCYC 3 cut(s) 267, 453, 755
Fsp4HI GCNGC 2 cut(s) 382, 1264
GlaI GCGC 1 cut(s) 158
GluI GCNGC 2 cut(s) 382, 1264
GsaI CCCAGC 4 cut(s) 85, 240, 914, 1001
GsuI CTGGAG 2 cut(s) 464, 827
HaeIII GGCC 2 cut(s) 110, 192
HgaI GACGC 3 cut(s) 112, 190, 229
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 1 cut(s) 1351
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 2 cut(s) 586, 600
HindII GTYRAC 2 cut(s) 586, 600
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 9 cut(s) 131, 566, 601, 631, 710, 922, 1094, 1304, 1387
HphI GGTGA 3 cut(s) 22, 502, 649
Hpy166II GTNNAC 4 cut(s) 118, 253, 586, 600
Hpy188I TCNGA 9 cut(s) 262, 286, 534, 730, 868, 897, 1396, 1423, 1431
Hpy188III TCNNGA 5 cut(s) 443, 609, 671, 926, 1076
Hpy8I GTNNAC 4 cut(s) 118, 253, 586, 600
Hpy99I CGWCG 3 cut(s) 176, 206, 245
HpyAV CCTTC 6 cut(s) 358, 516, 658, 700, 1055, 1249
HpyCH4III ACNGT 3 cut(s) 250, 400, 889
HpyCH4IV ACGT 4 cut(s) 147, 255, 279, 558
HpyCH4V TGCA 4 cut(s) 1229, 1263, 1330, 1363
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 189, 198, 207, 739, 916
HpySE526I ACGT 4 cut(s) 147, 255, 279, 558
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 1 cut(s) 1351
HspAI GCGC 1 cut(s) 157
Kpn2I TCCGGA 2 cut(s) 608, 925
KpnI GGTACC 1 cut(s) 1036
Kzo9I GATC 1 cut(s) 232
LguI GCTCTTC 1 cut(s) 374
Lsp1109I GCAGC 2 cut(s) 368, 1275
MaeII ACGT 4 cut(s) 147, 255, 279, 558
MaeIII GTNAC 1 cut(s) 244
MalI GATC 1 cut(s) 234
MbiI CCGCTC 2 cut(s) 334, 487
MboI GATC 1 cut(s) 232
MhlI GDGCHC 4 cut(s) 267, 453, 755, 1170
MluCI AATT 8 cut(s) 18, 505, 960, 1111, 1160, 1282, 1376, 1425
MlyI GAGTC 3 cut(s) 125, 595, 704
MmeI TCCRAC 2 cut(s) 309, 846
MroI TCCGGA 2 cut(s) 608, 925
MroXI GAANNNNTTC 1 cut(s) 689
MseI TTAA 4 cut(s) 518, 1110, 1130, 1220
MslI CAYNNNNRTG 1 cut(s) 1352
MspA1I CMGCKG 2 cut(s) 381, 880
MspCI CTTAAG 2 cut(s) 517, 1129
MspR9I CCNGG 5 cut(s) 154, 538, 590, 677, 779
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 3 cut(s) 154, 590, 677
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 6 cut(s) 107, 189, 198, 207, 739, 916
NciI CCSGG 2 cut(s) 538, 779
NcoI CCATGG 1 cut(s) 1347
NdeII GATC 1 cut(s) 232
NlaIII CATG 1 cut(s) 1351
NlaIV GGNNCC 4 cut(s) 193, 411, 722, 1034
NmeAIII GCCGAG 2 cut(s) 127, 601
PceI AGGCCT 1 cut(s) 110
PciSI GCTCTTC 1 cut(s) 374
PctI GAATGC 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 689
PfeI GAWTC 6 cut(s) 566, 631, 922, 1094, 1304, 1387
PfoI TCCNGGA 1 cut(s) 777
PkrI GCNGC 2 cut(s) 383, 1265
PleI GAGTC 3 cut(s) 125, 595, 704
PpsI GAGTC 3 cut(s) 125, 595, 704
PshBI ATTAAT 1 cut(s) 1110
Psp124BI GAGCTC 3 cut(s) 267, 453, 755
Psp6I CCWGG 3 cut(s) 152, 588, 675
PspFI CCCAGC 4 cut(s) 81, 236, 910, 997
PspGI CCWGG 3 cut(s) 152, 588, 675
PspN4I GGNNCC 4 cut(s) 193, 411, 722, 1034
PspPI GGNCC 3 cut(s) 191, 409, 817
PsrI GAACNNNNNNTAC 2 cut(s) 533, 565
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 3 cut(s) 278, 561, 1034
RsaNI GTAC 3 cut(s) 277, 560, 1033
RseI CAYNNNNRTG 1 cut(s) 1352
SacI GAGCTC 3 cut(s) 267, 453, 755
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 4 cut(s) 518, 1110, 1130, 1220
SatI GCNGC 2 cut(s) 382, 1264
Sau3AI GATC 1 cut(s) 232
Sau96I GGNCC 3 cut(s) 191, 409, 817
SchI GAGTC 3 cut(s) 125, 595, 704
ScrFI CCNGG 5 cut(s) 154, 538, 590, 677, 779
SduI GDGCHC 4 cut(s) 267, 453, 755, 1170
SinI GGWCC 2 cut(s) 409, 817
SmiMI CAYNNNNRTG 1 cut(s) 1352
SmlI CTYRAG 2 cut(s) 517, 1129
SmoI CTYRAG 2 cut(s) 517, 1129
Sse9I AATT 8 cut(s) 18, 505, 960, 1111, 1160, 1282, 1376, 1425
SseBI AGGCCT 1 cut(s) 110
SstI GAGCTC 3 cut(s) 267, 453, 755
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 5 cut(s) 152, 536, 588, 675, 777
StyI CCWWGG 2 cut(s) 1036, 1347
TaaI ACNGT 3 cut(s) 250, 400, 889
TaiI ACGT 4 cut(s) 150, 258, 282, 561
TaqI TCGA 2 cut(s) 129, 672
TaqII GACCGA 1 cut(s) 397
TasI AATT 8 cut(s) 18, 505, 960, 1111, 1160, 1282, 1376, 1425
TfiI GAWTC 6 cut(s) 566, 631, 922, 1094, 1304, 1387
Tru1I TTAA 4 cut(s) 518, 1110, 1130, 1220
Tru9I TTAA 4 cut(s) 518, 1110, 1130, 1220
TscAI CASTG 4 cut(s) 235, 496, 1074, 1332
TseI GCWGC 2 cut(s) 381, 1263
TspDTI ATGAA 1 cut(s) 1190
TspGWI ACGGA 3 cut(s) 103, 491, 559
TspRI CASTG 4 cut(s) 235, 496, 1074, 1332
Vha464I CTTAAG 2 cut(s) 517, 1129
VpaK11BI GGWCC 2 cut(s) 409, 817
VspI ATTAAT 1 cut(s) 1110
XapI RAATTY 3 cut(s) 1160, 1376, 1425
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.