RLG00000012487

Piriformospora indica-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011930
Physical Location & Seq
Reverse (-)
19208559 .. 19209953
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012487

Sequence Viewer

Length: 1395 bp
ATGGCTCAGCTCCACCCAATTTCCACATTCACCCTCCTCTTCCTCACCACCCTCCTGTCCTCTTTCGTCATTTCCCACCCCCAGCCCCTCCTCAGCCCCGCCGAGCAGGCCTCCGTCTACCGCGTCCTCGACTCCCTCAACCCCGACGTCCCCTGGCGCACCCTCTTCCCCGACGACCTCTGCTCCTACGGCCCCCACGGCGTCGTCTGCGACTTCTTCGCCAACGACACTGATCCCAGCGTAGTAACCGTCCACGTCTCCGAGCTCAGCTTCGGCTACGTCTCCGACTACACCCCCAACCCGCCGTGCTCCTCCACCGCCACCCTCAGCCCGCTCCTCTTCTCCGCCTTCCCTTACCTCCGCAAGCTCTTCTTCTACCGCTGCTTCAACGAAACGACGGTCGTTTCGGTCCCTGATATTCCGGCGAGTTTCGGGTCGGGTCTGGAGGAGCTCGCCTTTATAGACAACCCCTCTCTTGTTTCCCCGCTCAGTGGCATTCTCCGTAATTTCACCAACTTAAGAAGGGCGGTTCTGACCGGGAACGGGTTTTACGGCAACGTACCGGATTCTGTTGCCGAGATGGTCAACCTGGAAGAGTTGACTCTCTCCGGAAACCAACTCGGTGGAGAGATTCCGGTGAGTTTTGAAAAACTGAAGAAGCTGAAGGTTCTCGACCTGGGCGGCAACTCCTTCGCAGGAAAAGTTCCGGAATCGGTGGGGAGCCTGTCGGAGCTGTTGAAGCTTGACCTGAGCTCCAATGGGTTTTCCGGCGAAATCCCGGAGAGCCTCAAAAACTTGCGGAGGCTGGAGCTGTTGGACCTGAGCTACAACCAGTTCAACAACTCCGGCGTGCCTCTGTTCTTGTCGGAGATGACACAGCTGAGAACGGTGTCTCTGAGCGGGAATTTGCTAGGAGGGCAGATTCCGGAGATATGGAGCAAGCTCGGGGGCATTTTGGGAATTGGGCTTTCTAGGATGGGACTGGTTGGGAAAATCCCAGCTTCAATGGGGGTGTATTTGAGAAATGTGAGGTACCTTGGGCTTGACAACAACAAACTGGAAGGGACAGTGCCTCAGGAGTTTGGTCTTTTGGAATCTGTGAATGAGATTAATTTGGAGAACAATAGCTTAAGTGGGAGGGTCTCATTTTCTTCAGCTAAATTTGGGCACAAGATGAAGTTGTATGGAAACCCACAGCTCTGTGTTGACGATAAGGGTTTAAGGTTTGCAAAGGGTTTAGGGTTTGAGGAGTTGAAGGTGTGCAGCAAACAGCAGAAACCCAATTCTGCCCATTTCACAATGGATTCTTCTTCTTCTCCAGCAGTGCAGGTTGCTGTTCCATCCATGGTAATGGTTTGTGGGTTTTTGGTTATGATTGTGCTTATGCTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

465

Amino Acids

50.42

Weight (kDa)

5.39

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 172 - 230 5.9e-06 Leucine rich repeat
LRR_14 PF23598 188 - 299 6e-10 Leucine-rich repeat region
LRR_8 PF13855 196 - 254 4.1e-08 Leucine rich repeat
LRR_4 PF12799 196 - 229 3.6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28560
fragaria_vesca FvH4_2g21070
malus_domestica MD10G1180100.v1.1
prunus_persica Prupe.8G220100_v2.0.a1
pyrus_communis pycom05g18060 pycom10g15600
rosa_chinensis RchiOBHm_Chr6g0287531
rosa_laevigata RLG00000012487
rosa_multiflora Rmu_sc0004974.1_g000008 Rmu_sc0007029.1_g000010
rosa_roxburghii Rroxscaffold_7G00180930 Rroxscaffold_7G00181400
rosa_rugosa Rorug06G0188700
rosa_samantha Rh6AG300200 Rh6BG305500 Rh6CG304000 Rh6CG314000 Rh6DG297400
rosa_wichuraiana Rw6G025880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
Acc36I ACCTGC 1 cut(s) 1318
Acc65I GGTACC 1 cut(s) 1032
AccB1I GGYRCC 1 cut(s) 1032
AccBSI CCGCTC 3 cut(s) 334, 487, 900
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 123
AccIII TCCGGA 3 cut(s) 608, 706, 925
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 2 cut(s) 904, 1160
AcuI CTGAAG 3 cut(s) 674, 683, 1137
AcyI GRCGYC 2 cut(s) 147, 201
AfaI GTAC 2 cut(s) 561, 1034
AfiI CCNNNNNNNGG 2 cut(s) 491, 543
AflII CTTAAG 2 cut(s) 517, 1129
AgsI TTSAA 6 cut(s) 388, 647, 739, 838, 1005, 1255
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 3 cut(s) 152, 588, 675
Alw21I GWGCWC 4 cut(s) 267, 311, 453, 755
Alw26I GTCTC 4 cut(s) 262, 286, 897, 1147
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 944
Aor13HI TCCGGA 3 cut(s) 608, 706, 925
AoxI GGCC 2 cut(s) 108, 190
ApeKI GCWGC 2 cut(s) 381, 1263
ApoI RAATTY 2 cut(s) 904, 1160
ArsI GACNNNNNNTTYG 4 cut(s) 384, 388, 416, 420
AseI ATTAAT 1 cut(s) 1110
Asp718I GGTACC 1 cut(s) 1032
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 3 cut(s) 191, 409, 817
AsuC2I CCSGG 2 cut(s) 538, 779
AsuHPI GGTGA 4 cut(s) 22, 37, 502, 649
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 2 cut(s) 409, 817
AxyI CCTNAGG 1 cut(s) 1074
BaeGI GKGCMC 1 cut(s) 1170
BanI GGYRCC 1 cut(s) 1032
BanII GRGCYC 3 cut(s) 267, 453, 755
Bbv12I GWGCWC 4 cut(s) 267, 311, 453, 755
BbvCI CCTCAGC 2 cut(s) 92, 326
BbvI GCAGC 2 cut(s) 368, 1275
BccI CCATC 3 cut(s) 574, 970, 1348
BceAI ACGGC 4 cut(s) 205, 214, 289, 568
BcgI CGANNNNNNTGC 2 cut(s) 673, 707
BciT130I CCWGG 3 cut(s) 154, 590, 677
BcnI CCSGG 2 cut(s) 538, 779
BcoDI GTCTC 4 cut(s) 262, 286, 897, 1147
BfaI CTAG 2 cut(s) 911, 972
BfrI CTTAAG 2 cut(s) 517, 1129
BfuAI ACCTGC 1 cut(s) 1318
BglI GCCNNNNNGGC 2 cut(s) 107, 198
BisI GCNGC 3 cut(s) 382, 682, 1264
BlpI GCTNAGC 2 cut(s) 6, 266
BlsI GCNGC 3 cut(s) 383, 683, 1265
Bme1390I CCNGG 5 cut(s) 154, 538, 590, 677, 779
Bme18I GGWCC 2 cut(s) 409, 817
BmeT110I CYCGRG 1 cut(s) 944
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 3 cut(s) 191, 409, 817
BmiI GGNNCC 4 cut(s) 193, 411, 722, 1034
BmrFI CCNGG 5 cut(s) 154, 538, 590, 677, 779
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 3 cut(s) 464, 827, 1302
Bpu10I CCTNAGC 4 cut(s) 92, 326, 749, 821
Bpu1102I GCTNAGC 2 cut(s) 6, 266
BpuMI CCSGG 2 cut(s) 538, 779
BsaHI GRCGYC 2 cut(s) 147, 201
BsaI GGTCTC 1 cut(s) 1147
BsaJI CCNNGG 5 cut(s) 152, 196, 676, 1036, 1344
BsaWI WCCGGW 5 cut(s) 562, 608, 634, 706, 925
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 737, 767
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 543
Bse1I ACTGG 3 cut(s) 832, 987, 1062
Bse21I CCTNAGG 1 cut(s) 1074
BseAI TCCGGA 3 cut(s) 608, 706, 925
BseBI CCWGG 3 cut(s) 154, 590, 677
BseDI CCNNGG 5 cut(s) 152, 196, 676, 1036, 1344
BseGI GGATG 2 cut(s) 981, 1340
BseLI CCNNNNNNNGG 2 cut(s) 491, 543
BseNI ACTGG 3 cut(s) 832, 987, 1062
BseRI GAGGAG 6 cut(s) 26, 80, 301, 326, 461, 1262
BseSI GKGCMC 1 cut(s) 1170
BseXI GCAGC 2 cut(s) 368, 1275
BseYI CCCAGC 3 cut(s) 81, 236, 997
BsgI GTGCAG 2 cut(s) 1282, 1346
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 2 cut(s) 110, 192
BshNI GGYRCC 1 cut(s) 1032
BsiEI CGRYCG 1 cut(s) 402
BsiHKAI GWGCWC 4 cut(s) 267, 311, 453, 755
BsiHKCI CYCGRG 1 cut(s) 944
BslFI GGGAC 4 cut(s) 134, 395, 993, 1078
BslI CCNNNNNNNGG 2 cut(s) 491, 543
BsmAI GTCTC 4 cut(s) 262, 286, 897, 1147
BsmBI CGTCTC 2 cut(s) 262, 286
BsmFI GGGAC 4 cut(s) 134, 395, 993, 1078
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 2 cut(s) 110, 192
Bso31I GGTCTC 1 cut(s) 1147
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 5 cut(s) 267, 311, 453, 755, 1170
Bsp13I TCCGGA 3 cut(s) 608, 706, 925
Bsp143I GATC 1 cut(s) 232
Bsp1720I GCTNAGC 2 cut(s) 6, 266
Bsp19I CCATGG 1 cut(s) 1344
BspANI GGCC 2 cut(s) 110, 192
BspEI TCCGGA 3 cut(s) 608, 706, 925
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 4 cut(s) 193, 411, 722, 1034
BspMI ACCTGC 1 cut(s) 1318
BspPI GGATC 1 cut(s) 227
BspQI GCTCTTC 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 1032
BspTI CTTAAG 2 cut(s) 517, 1129
BspTNI GGTCTC 1 cut(s) 1147
BsrBI CCGCTC 3 cut(s) 334, 487, 900
BsrI ACTGG 3 cut(s) 832, 987, 1062
BssECI CCNNGG 5 cut(s) 152, 196, 676, 1036, 1344
BssMI GATC 1 cut(s) 232
BssNI GRCGYC 2 cut(s) 147, 201
BssT1I CCWWGG 2 cut(s) 1036, 1344
Bst2UI CCWGG 3 cut(s) 154, 590, 677
Bst4CI ACNGT 4 cut(s) 250, 400, 889, 1069
Bst6I CTCTTC 5 cut(s) 44, 170, 344, 374, 588
BstACI GRCGYC 2 cut(s) 147, 201
BstAFI CTTAAG 2 cut(s) 517, 1129
BstC8I GCNNGC 6 cut(s) 108, 332, 365, 453, 851, 941
BstDSI CCRYGG 2 cut(s) 196, 1344
BstF5I GGATG 2 cut(s) 981, 1340
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 1 cut(s) 235
BstMAI GTCTC 4 cut(s) 262, 286, 897, 1147
BstMBI GATC 1 cut(s) 232
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 6 cut(s) 107, 189, 198, 207, 739, 916
BstNI CCWGG 3 cut(s) 154, 590, 677
BstSCI CCNGG 5 cut(s) 152, 536, 588, 675, 777
BstSLI GKGCMC 1 cut(s) 1170
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 368, 1275
BstXI CCANNNNNNTGG 2 cut(s) 623, 1351
Bsu36I CCTNAGG 1 cut(s) 1074
BsuRI GGCC 2 cut(s) 110, 192
BtgI CCRYGG 2 cut(s) 196, 1344
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 2 cut(s) 981, 1340
BtsI GCAGTG 1 cut(s) 1329
BtsIMutI CAGTG 4 cut(s) 228, 496, 1074, 1329
BveI ACCTGC 1 cut(s) 1318
Cac8I GCNNGC 6 cut(s) 108, 332, 365, 453, 851, 941
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 3 cut(s) 191, 409, 817
CseI GACGC 2 cut(s) 112, 190
Csp6I GTAC 2 cut(s) 560, 1033
CviAII CATG 1 cut(s) 1345
CviQI GTAC 2 cut(s) 560, 1033
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
Eam1104I CTCTTC 5 cut(s) 44, 170, 344, 374, 588
EarI CTCTTC 5 cut(s) 44, 170, 344, 374, 588
EciI GGCGGA 1 cut(s) 334
Ecl136II GAGCTC 3 cut(s) 265, 451, 753
Eco130I CCWWGG 2 cut(s) 1036, 1344
Eco147I AGGCCT 1 cut(s) 110
Eco24I GRGCYC 3 cut(s) 267, 453, 755
Eco31I GGTCTC 1 cut(s) 1147
Eco47I GGWCC 2 cut(s) 409, 817
Eco53kI GAGCTC 3 cut(s) 265, 451, 753
Eco57I CTGAAG 3 cut(s) 674, 683, 1137
Eco81I CCTNAGG 1 cut(s) 1074
Eco88I CYCGRG 1 cut(s) 944
EcoICRI GAGCTC 3 cut(s) 265, 451, 753
EcoRII CCWGG 3 cut(s) 152, 588, 675
EcoT14I CCWWGG 2 cut(s) 1036, 1344
EcoT38I GRGCYC 3 cut(s) 267, 453, 755
ErhI CCWWGG 2 cut(s) 1036, 1344
Esp3I CGTCTC 2 cut(s) 262, 286
FaeI CATG 1 cut(s) 1348
FaiI YATR 6 cut(s) 461, 934, 1185, 1346, 1373, 1385
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 4 cut(s) 134, 395, 993, 1078
FatI CATG 1 cut(s) 1344
FauI CCCGC 5 cut(s) 106, 309, 339, 492, 893
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 3 cut(s) 382, 682, 1264
FokI GGATG 2 cut(s) 988, 1327
FriOI GRGCYC 3 cut(s) 267, 453, 755
Fsp4HI GCNGC 3 cut(s) 382, 682, 1264
FspBI CTAG 2 cut(s) 911, 972
GlaI GCGC 1 cut(s) 158
GluI GCNGC 3 cut(s) 382, 682, 1264
GsaI CCCAGC 3 cut(s) 85, 240, 1001
GsuI CTGGAG 3 cut(s) 464, 827, 1302
HaeIII GGCC 2 cut(s) 110, 192
HgaI GACGC 2 cut(s) 112, 190
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 2 cut(s) 147, 201
Hin1II CATG 1 cut(s) 1348
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 3 cut(s) 586, 600, 1207
HindII GTYRAC 3 cut(s) 586, 600, 1207
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 8 cut(s) 131, 566, 601, 631, 710, 922, 1094, 1304
HphI GGTGA 4 cut(s) 22, 37, 502, 649
Hpy166II GTNNAC 5 cut(s) 118, 253, 586, 600, 1207
Hpy188I TCNGA 6 cut(s) 262, 286, 534, 730, 868, 897
Hpy188III TCNNGA 6 cut(s) 443, 609, 671, 707, 926, 1076
Hpy8I GTNNAC 5 cut(s) 118, 253, 586, 600, 1207
Hpy99I CGWCG 4 cut(s) 149, 176, 206, 400
HpyAV CCTTC 6 cut(s) 358, 516, 658, 700, 1055, 1249
HpyCH4III ACNGT 4 cut(s) 250, 400, 889, 1069
HpyCH4IV ACGT 4 cut(s) 147, 255, 279, 558
HpyCH4V TGCA 3 cut(s) 1229, 1263, 1327
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 189, 198, 207, 739, 916
HpySE526I ACGT 4 cut(s) 147, 255, 279, 558
Hsp92I GRCGYC 2 cut(s) 147, 201
Hsp92II CATG 1 cut(s) 1348
HspAI GCGC 1 cut(s) 157
Kpn2I TCCGGA 3 cut(s) 608, 706, 925
KpnI GGTACC 1 cut(s) 1036
Kzo9I GATC 1 cut(s) 232
LguI GCTCTTC 1 cut(s) 374
Lsp1109I GCAGC 2 cut(s) 368, 1275
MaeI CTAG 2 cut(s) 911, 972
MaeII ACGT 4 cut(s) 147, 255, 279, 558
MaeIII GTNAC 1 cut(s) 244
MalI GATC 1 cut(s) 234
MbiI CCGCTC 3 cut(s) 334, 487, 900
MboI GATC 1 cut(s) 232
MhlI GDGCHC 5 cut(s) 267, 311, 453, 755, 1170
MluCI AATT 8 cut(s) 18, 505, 904, 960, 1111, 1160, 1282, 1390
MlyI GAGTC 2 cut(s) 125, 595
MmeI TCCRAC 4 cut(s) 309, 708, 795, 846
MroI TCCGGA 3 cut(s) 608, 706, 925
MseI TTAA 5 cut(s) 518, 1110, 1130, 1220, 1389
MslI CAYNNNNRTG 1 cut(s) 1349
MspA1I CMGCKG 2 cut(s) 381, 880
MspCI CTTAAG 2 cut(s) 517, 1129
MspR9I CCNGG 5 cut(s) 154, 538, 590, 677, 779
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 3 cut(s) 154, 590, 677
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 6 cut(s) 107, 189, 198, 207, 739, 916
NciI CCSGG 2 cut(s) 538, 779
NcoI CCATGG 1 cut(s) 1344
NdeII GATC 1 cut(s) 232
NlaIII CATG 1 cut(s) 1348
NlaIV GGNNCC 4 cut(s) 193, 411, 722, 1034
NmeAIII GCCGAG 2 cut(s) 127, 601
PceI AGGCCT 1 cut(s) 110
PciSI GCTCTTC 1 cut(s) 374
PctI GAATGC 1 cut(s) 495
PfeI GAWTC 6 cut(s) 566, 631, 710, 922, 1094, 1304
PfoI TCCNGGA 1 cut(s) 777
PkrI GCNGC 3 cut(s) 383, 683, 1265
PleI GAGTC 2 cut(s) 125, 595
PpsI GAGTC 2 cut(s) 125, 595
PshBI ATTAAT 1 cut(s) 1110
Psp124BI GAGCTC 3 cut(s) 267, 453, 755
Psp6I CCWGG 3 cut(s) 152, 588, 675
PspFI CCCAGC 3 cut(s) 81, 236, 997
PspGI CCWGG 3 cut(s) 152, 588, 675
PspN4I GGNNCC 4 cut(s) 193, 411, 722, 1034
PspPI GGNCC 3 cut(s) 191, 409, 817
PsrI GAACNNNNNNTAC 2 cut(s) 533, 565
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 2 cut(s) 561, 1034
RsaNI GTAC 2 cut(s) 560, 1033
RseI CAYNNNNRTG 1 cut(s) 1349
SacI GAGCTC 3 cut(s) 267, 453, 755
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 5 cut(s) 518, 1110, 1130, 1220, 1389
SatI GCNGC 3 cut(s) 382, 682, 1264
Sau3AI GATC 1 cut(s) 232
Sau96I GGNCC 3 cut(s) 191, 409, 817
SchI GAGTC 2 cut(s) 125, 595
ScrFI CCNGG 5 cut(s) 154, 538, 590, 677, 779
SduI GDGCHC 5 cut(s) 267, 311, 453, 755, 1170
SinI GGWCC 2 cut(s) 409, 817
SmiMI CAYNNNNRTG 1 cut(s) 1349
SmlI CTYRAG 2 cut(s) 517, 1129
SmoI CTYRAG 2 cut(s) 517, 1129
Sse9I AATT 8 cut(s) 18, 505, 904, 960, 1111, 1160, 1282, 1390
SseBI AGGCCT 1 cut(s) 110
SspMI CTAG 2 cut(s) 911, 972
SstI GAGCTC 3 cut(s) 267, 453, 755
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 5 cut(s) 152, 536, 588, 675, 777
StyI CCWWGG 2 cut(s) 1036, 1344
TaaI ACNGT 4 cut(s) 250, 400, 889, 1069
TaiI ACGT 4 cut(s) 150, 258, 282, 561
TaqI TCGA 2 cut(s) 129, 672
TaqII GACCGA 1 cut(s) 397
TasI AATT 8 cut(s) 18, 505, 904, 960, 1111, 1160, 1282, 1390
TauI GCSGC 1 cut(s) 684
TfiI GAWTC 6 cut(s) 566, 631, 710, 922, 1094, 1304
Tru1I TTAA 5 cut(s) 518, 1110, 1130, 1220, 1389
Tru9I TTAA 5 cut(s) 518, 1110, 1130, 1220, 1389
TscAI CASTG 4 cut(s) 235, 496, 1074, 1329
TseI GCWGC 2 cut(s) 381, 1263
TspDTI ATGAA 1 cut(s) 1190
TspGWI ACGGA 2 cut(s) 103, 491
TspRI CASTG 4 cut(s) 235, 496, 1074, 1329
Vha464I CTTAAG 2 cut(s) 517, 1129
VpaK11BI GGWCC 2 cut(s) 409, 817
VspI ATTAAT 1 cut(s) 1110
XapI RAATTY 2 cut(s) 904, 1160
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 2 cut(s) 911, 972
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.