Rh6DG297400

Piriformospora indica-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011930
Physical Location & Seq
Forward (+)
49930023 .. 49931828
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG297400.1

Sequence Viewer

Length: 1044 bp
ATGGCTCAGCTCCACCCAATTTCCACATTCACCCTCCTCTTCCTCACAACCCTCCTGTCCTCTTTCGTCATTTCCCACTCCCAGCCCCTCCTCAGCCCGGCCGAGCAGGCCTCCGTCTACCGCGTCCTCGACTCCCTCAACCCCCACGTCCCCTGGCGCACCCTCTTCCCCGACGACCTCTGCTCCTACGGCCCCCACGGTGTCGTCTGCGACTTCTTCGCCAACGACACTGATCCCAGCGTCGTAACCGTCCACGTCTCCGAGCTCAGCTTCGGGTACGTCTCCGACTACACCCCCAACCCGCCCTGCTCCTCCACCGCCACTCTCAGCCCTCTCCTCTTCACTTCCTTCCCTTACCTCCGCAAGCTCTTCTTCTACCGCTGCTTCAACGAAACGACAGTCGTTTCGGTCCCTGATATTCCGGCGAGTTTCGGGTCGGGTCTGGAGGAGCTCGTCTTTATAGACAACCCCTCTCTTGTTTCCCCGCTCAGTGGCATTCTCCGTAATTTCACCAGCTTAAGAAGGGCAGTTCTGACCGGGAACGGGTTTTACGGCAACGTACCGGATTCCGTTGCCGAGATGGTCCACCTGGAAGAATTGACTCTCTCCGGAAACCAACTCGGTGGAGAGATTCCGGTGAGTTTTGAAAAACTGAAGAAGCTGAAGGTTCTCGACCTGGGCGGGAACTCCTTCGCCGGAAAAGTGCCGGACTCGGTGGGGAGCCTCTCGGAGCTGTTGAAGCTTGACCTGAGCTCCAATGGGTTTTCCGGCAAAATCCCGGAGAGCCTCAAAAACTTGCGGAGGCTGGAGCTGTTGGACCTGAGCTACAACCGGTTCAACAACTCCGGCGTGCCTCTGTTCTTGTCGGAGATGACACAGCTGAGAACGGTGTCTCTGAGTGGGAATTTGCTAGGAGGGCAGATTCCGGAAATATGGAGCAAGCTCGGGGGCATTTTGGGAATTGGGCTTTCTGGGATGGGACTGGTTGGGAAAATCCCAGCTTCAATGGGGGTGTATTTGAGAAATGTGAGAGATTCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

347

Amino Acids

37.61

Weight (kDa)

5.54

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 173 - 230 8.3e-06 Leucine rich repeat
LRR_14 PF23598 186 - 299 4.9e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28560
fragaria_vesca FvH4_2g21070
malus_domestica MD10G1180100.v1.1
prunus_persica Prupe.8G220100_v2.0.a1
pyrus_communis pycom05g18060 pycom10g15600
rosa_chinensis RchiOBHm_Chr6g0287531
rosa_laevigata RLG00000012487
rosa_multiflora Rmu_sc0004974.1_g000008 Rmu_sc0007029.1_g000010
rosa_roxburghii Rroxscaffold_7G00180930 Rroxscaffold_7G00181400
rosa_rugosa Rorug06G0188700
rosa_samantha Rh6AG300200 Rh6BG305500 Rh6CG304000 Rh6CG314000 Rh6DG297400
rosa_wichuraiana Rw6G025880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 123
AccIII TCCGGA 2 cut(s) 608, 925
AciI CCGC 8 cut(s) 121, 302, 318, 361, 379, 485, 681, 799
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 1 cut(s) 904
AcuI CTGAAG 2 cut(s) 674, 683
AfaI GTAC 2 cut(s) 278, 561
AfiI CCNNNNNNNGG 3 cut(s) 97, 491, 543
AflII CTTAAG 1 cut(s) 517
AgeI ACCGGT 1 cut(s) 831
AgsI TTSAA 5 cut(s) 388, 647, 739, 838, 1005
AjiI CACGTC 2 cut(s) 148, 256
AjnI CCWGG 3 cut(s) 152, 588, 675
Alw21I GWGCWC 3 cut(s) 267, 453, 755
Alw26I GTCTC 3 cut(s) 262, 286, 897
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 944
Aor13HI TCCGGA 2 cut(s) 608, 925
AoxI GGCC 3 cut(s) 99, 108, 190
ApeKI GCWGC 1 cut(s) 381
ApoI RAATTY 1 cut(s) 904
ArsI GACNNNNNNTTYG 4 cut(s) 384, 388, 416, 420
AsiGI ACCGGT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 689
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 4 cut(s) 191, 409, 583, 817
AsuC2I CCSGG 3 cut(s) 98, 538, 779
AsuHPI GGTGA 3 cut(s) 22, 502, 649
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 3 cut(s) 409, 583, 817
BanII GRGCYC 3 cut(s) 267, 453, 755
Bbv12I GWGCWC 3 cut(s) 267, 453, 755
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 368
BccI CCATC 2 cut(s) 574, 970
BceAI ACGGC 2 cut(s) 205, 568
BciT130I CCWGG 3 cut(s) 154, 590, 677
BcnI CCSGG 3 cut(s) 98, 538, 779
BcoDI GTCTC 3 cut(s) 262, 286, 897
BfaI CTAG 1 cut(s) 911
BfrI CTTAAG 1 cut(s) 517
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 382
BlpI GCTNAGC 2 cut(s) 6, 266
BlsI GCNGC 1 cut(s) 383
Bme1390I CCNGG 6 cut(s) 98, 154, 538, 590, 677, 779
Bme18I GGWCC 3 cut(s) 409, 583, 817
BmeT110I CYCGRG 1 cut(s) 944
BmgBI CACGTC 2 cut(s) 148, 256
BmgT120I GGNCC 4 cut(s) 191, 409, 583, 817
BmiI GGNNCC 3 cut(s) 193, 411, 722
BmrFI CCNGG 6 cut(s) 98, 154, 538, 590, 677, 779
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 2 cut(s) 464, 827
Bpu10I CCTNAGC 3 cut(s) 92, 749, 821
Bpu1102I GCTNAGC 2 cut(s) 6, 266
BpuMI CCSGG 3 cut(s) 98, 538, 779
BsaJI CCNNGG 3 cut(s) 152, 196, 676
BsaWI WCCGGW 5 cut(s) 562, 608, 634, 831, 925
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 737, 767
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 491, 543
Bse118I RCCGGY 1 cut(s) 831
Bse1I ACTGG 1 cut(s) 987
BseAI TCCGGA 2 cut(s) 608, 925
BseBI CCWGG 3 cut(s) 154, 590, 677
BseDI CCNNGG 3 cut(s) 152, 196, 676
BseGI GGATG 1 cut(s) 981
BseLI CCNNNNNNNGG 3 cut(s) 97, 491, 543
BseMII CTCAG 9 cut(s) 20, 106, 280, 340, 502, 740, 812, 872, 887
BseNI ACTGG 1 cut(s) 987
BseRI GAGGAG 5 cut(s) 26, 80, 301, 326, 461
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 368
BseYI CCCAGC 3 cut(s) 81, 236, 997
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 3 cut(s) 101, 110, 192
BshTI ACCGGT 1 cut(s) 831
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 3 cut(s) 267, 453, 755
BsiHKCI CYCGRG 1 cut(s) 944
BslFI GGGAC 3 cut(s) 134, 395, 993
BslI CCNNNNNNNGG 3 cut(s) 97, 491, 543
BsmAI GTCTC 3 cut(s) 262, 286, 897
BsmBI CGTCTC 2 cut(s) 262, 286
BsmFI GGGAC 3 cut(s) 134, 395, 993
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 3 cut(s) 101, 110, 192
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 3 cut(s) 267, 453, 755
Bsp13I TCCGGA 2 cut(s) 608, 925
Bsp143I GATC 1 cut(s) 232
Bsp1720I GCTNAGC 2 cut(s) 6, 266
BspACI CCGC 8 cut(s) 121, 302, 318, 361, 379, 485, 681, 799
BspANI GGCC 3 cut(s) 101, 110, 192
BspCNI CTCAG 9 cut(s) 19, 105, 279, 339, 501, 741, 813, 873, 888
BspEI TCCGGA 2 cut(s) 608, 925
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 193, 411, 722
BspPI GGATC 1 cut(s) 227
BspQI GCTCTTC 1 cut(s) 374
BspTI CTTAAG 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 831
BsrI ACTGG 1 cut(s) 987
BssAI RCCGGY 1 cut(s) 831
BssECI CCNNGG 3 cut(s) 152, 196, 676
BssMI GATC 1 cut(s) 232
Bst2UI CCWGG 3 cut(s) 154, 590, 677
Bst4CI ACNGT 4 cut(s) 200, 250, 400, 889
Bst6I CTCTTC 4 cut(s) 44, 170, 344, 374
BstAFI CTTAAG 1 cut(s) 517
BstC8I GCNNGC 4 cut(s) 108, 365, 851, 941
BstDEI CTNAG 9 cut(s) 6, 92, 266, 326, 488, 749, 821, 881, 896
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 1 cut(s) 981
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 1 cut(s) 235
BstMAI GTCTC 3 cut(s) 262, 286, 897
BstMBI GATC 1 cut(s) 232
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 4 cut(s) 107, 189, 739, 916
BstNI CCWGG 3 cut(s) 154, 590, 677
BstSCI CCNGG 6 cut(s) 96, 152, 536, 588, 675, 777
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 368
BstXI CCANNNNNNTGG 1 cut(s) 623
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 3 cut(s) 101, 110, 192
BtgI CCRYGG 1 cut(s) 196
BtrI CACGTC 2 cut(s) 148, 256
BtsCI GGATG 1 cut(s) 981
BtsIMutI CAGTG 2 cut(s) 228, 496
Cac8I GCNNGC 4 cut(s) 108, 365, 851, 941
CfoI GCGC 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 831
Cfr13I GGNCC 4 cut(s) 191, 409, 583, 817
CseI GACGC 2 cut(s) 112, 229
Csp6I GTAC 2 cut(s) 277, 560
CspAI ACCGGT 1 cut(s) 831
CviQI GTAC 2 cut(s) 277, 560
DdeI CTNAG 9 cut(s) 6, 92, 266, 326, 488, 749, 821, 881, 896
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 99
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 4 cut(s) 44, 170, 344, 374
EarI CTCTTC 4 cut(s) 44, 170, 344, 374
Ecl136II GAGCTC 3 cut(s) 265, 451, 753
EclXI CGGCCG 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 110
Eco24I GRGCYC 3 cut(s) 267, 453, 755
Eco47I GGWCC 3 cut(s) 409, 583, 817
Eco52I CGGCCG 1 cut(s) 99
Eco53kI GAGCTC 3 cut(s) 265, 451, 753
Eco57I CTGAAG 2 cut(s) 674, 683
Eco88I CYCGRG 1 cut(s) 944
EcoICRI GAGCTC 3 cut(s) 265, 451, 753
EcoRII CCWGG 3 cut(s) 152, 588, 675
EcoT38I GRGCYC 3 cut(s) 267, 453, 755
Esp3I CGTCTC 2 cut(s) 262, 286
FaiI YATR 2 cut(s) 461, 934
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 3 cut(s) 134, 395, 993
FauI CCCGC 3 cut(s) 309, 492, 674
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 1 cut(s) 382
FokI GGATG 1 cut(s) 988
FriOI GRGCYC 3 cut(s) 267, 453, 755
Fsp4HI GCNGC 1 cut(s) 382
FspBI CTAG 1 cut(s) 911
GlaI GCGC 1 cut(s) 158
GluI GCNGC 1 cut(s) 382
GsaI CCCAGC 3 cut(s) 85, 240, 1001
GsuI CTGGAG 2 cut(s) 464, 827
HaeIII GGCC 3 cut(s) 101, 110, 192
HgaI GACGC 2 cut(s) 112, 229
HhaI GCGC 1 cut(s) 159
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 7 cut(s) 131, 566, 601, 631, 710, 922, 1034
HphI GGTGA 3 cut(s) 22, 502, 649
Hpy166II GTNNAC 3 cut(s) 118, 253, 586
Hpy188I TCNGA 7 cut(s) 262, 286, 534, 730, 868, 897, 1043
Hpy188III TCNNGA 4 cut(s) 443, 609, 671, 926
Hpy8I GTNNAC 3 cut(s) 118, 253, 586
Hpy99I CGWCG 2 cut(s) 176, 245
HpyAV CCTTC 4 cut(s) 358, 516, 658, 700
HpyCH4III ACNGT 4 cut(s) 200, 250, 400, 889
HpyCH4IV ACGT 4 cut(s) 147, 255, 279, 558
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 189, 739, 916
HpyF3I CTNAG 9 cut(s) 6, 92, 266, 326, 488, 749, 821, 881, 896
HpySE526I ACGT 4 cut(s) 147, 255, 279, 558
HspAI GCGC 1 cut(s) 157
Kpn2I TCCGGA 2 cut(s) 608, 925
Kzo9I GATC 1 cut(s) 232
LguI GCTCTTC 1 cut(s) 374
LmnI GCTCC 9 cut(s) 15, 188, 314, 448, 720, 730, 758, 808, 936
Lsp1109I GCAGC 1 cut(s) 368
MaeI CTAG 1 cut(s) 911
MaeII ACGT 4 cut(s) 147, 255, 279, 558
MaeIII GTNAC 1 cut(s) 244
MalI GATC 1 cut(s) 234
MbiI CCGCTC 1 cut(s) 487
MboI GATC 1 cut(s) 232
MboII GAAGA 8 cut(s) 31, 157, 208, 331, 361, 364, 605, 667
MhlI GDGCHC 3 cut(s) 267, 453, 755
MluCI AATT 5 cut(s) 18, 505, 596, 904, 960
MlyI GAGTC 3 cut(s) 125, 595, 704
MmeI TCCRAC 3 cut(s) 309, 795, 846
MroI TCCGGA 2 cut(s) 608, 925
MroXI GAANNNNTTC 1 cut(s) 689
MseI TTAA 1 cut(s) 518
MspA1I CMGCKG 2 cut(s) 381, 880
MspCI CTTAAG 1 cut(s) 517
MspR9I CCNGG 6 cut(s) 98, 154, 538, 590, 677, 779
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 3 cut(s) 154, 590, 677
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 4 cut(s) 107, 189, 739, 916
NciI CCSGG 3 cut(s) 98, 538, 779
NdeII GATC 1 cut(s) 232
NlaIV GGNNCC 3 cut(s) 193, 411, 722
NmeAIII GCCGAG 2 cut(s) 127, 601
PceI AGGCCT 1 cut(s) 110
PciSI GCTCTTC 1 cut(s) 374
PctI GAATGC 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 689
PfeI GAWTC 4 cut(s) 566, 631, 922, 1034
PfoI TCCNGGA 1 cut(s) 777
PinAI ACCGGT 1 cut(s) 831
PkrI GCNGC 1 cut(s) 383
PleI GAGTC 3 cut(s) 125, 595, 704
PpsI GAGTC 3 cut(s) 125, 595, 704
Psp124BI GAGCTC 3 cut(s) 267, 453, 755
Psp6I CCWGG 3 cut(s) 152, 588, 675
PspFI CCCAGC 3 cut(s) 81, 236, 997
PspGI CCWGG 3 cut(s) 152, 588, 675
PspN4I GGNNCC 3 cut(s) 193, 411, 722
PspPI GGNCC 4 cut(s) 191, 409, 583, 817
PsrI GAACNNNNNNTAC 2 cut(s) 533, 565
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 2 cut(s) 278, 561
RsaNI GTAC 2 cut(s) 277, 560
SacI GAGCTC 3 cut(s) 267, 453, 755
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 1 cut(s) 382
Sau3AI GATC 1 cut(s) 232
Sau96I GGNCC 4 cut(s) 191, 409, 583, 817
SchI GAGTC 3 cut(s) 125, 595, 704
ScrFI CCNGG 6 cut(s) 98, 154, 538, 590, 677, 779
SduI GDGCHC 3 cut(s) 267, 453, 755
SfiI GGCCNNNNNGGCC 1 cut(s) 107
SinI GGWCC 3 cut(s) 409, 583, 817
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
Sse9I AATT 5 cut(s) 18, 505, 596, 904, 960
SseBI AGGCCT 1 cut(s) 110
SsiI CCGC 8 cut(s) 121, 302, 318, 361, 379, 485, 681, 799
SspMI CTAG 1 cut(s) 911
SstI GAGCTC 3 cut(s) 267, 453, 755
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 6 cut(s) 96, 152, 536, 588, 675, 777
TaaI ACNGT 4 cut(s) 200, 250, 400, 889
TaiI ACGT 4 cut(s) 150, 258, 282, 561
TaqI TCGA 2 cut(s) 129, 672
TaqII GACCGA 1 cut(s) 397
TasI AATT 5 cut(s) 18, 505, 596, 904, 960
TfiI GAWTC 4 cut(s) 566, 631, 922, 1034
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TscAI CASTG 2 cut(s) 235, 496
TseI GCWGC 1 cut(s) 381
TspGWI ACGGA 3 cut(s) 103, 491, 559
TspRI CASTG 2 cut(s) 235, 496
Vha464I CTTAAG 1 cut(s) 517
VpaK11BI GGWCC 3 cut(s) 409, 583, 817
XapI RAATTY 1 cut(s) 904
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 1 cut(s) 689
XspI CTAG 1 cut(s) 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.