Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009171

Physical Location & Seq

Genomic Coordinates Reverse (-)

32162964 .. 32165924

Gene Structure 2961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1223700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 678 bp

ATGAAGCAGCAATCCAAGGACCCATTCGAAGCGGCGTTCGAGGAGCAAGAAGACGACTCACCGCCCGATTCTCCGGCCGTCGCTGAAGCCGCGCGCGCCGCCTTTGGTGCCCTAGAAGAGGACGACCCTCTTCTTGTTCCTGGCCCGTCATCTTCCTCCGCGCCATCAGCTGCAGCGCCGAAAGCAACAGCAGTTACCAGAACAAGAACAACGCCAACTGCTGCTGCGACGGCGCCCTCCGCCTCTGCTCGGCCCGCTGTCAAGAACAAGGATGACGACGACGATGACGACGAAGACAACATGGATGTCGAGCTCGGAAAGTTCTCCGCCACCGGTGATCCTGACAAAATGGCCAAGATGCAGGCTATTTTATCGCAATTCTCAGAGGAACAGATGAGTAGGTACGAGTCGTTTCGCAGAGCTGGATTTCAGAGAGCCAACATGAAAAGGTTGTTAACTAGCATCAGTGGAACTCCAAAAATATCTGCGCCAATGAATATTGTAGTGTCAGGGATAGCAAAGATGTTTGTTGGTGAACTTGTTGAGACAGCCAGAATAGTGATGTCAGAGAGGAAAGAGTCTGGGCCAATCAGGCCATGCCACCTTAGAGAAGCATACAGAAGGTTGAAGCTTGAAGGGAAGGTACCAAAGAGATCACAGTCTAGGCTCTTCCGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0006139 GO:0006351 GO:0006352 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010556 GO:0010557 GO:0010604 GO:0016043 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051123 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0060255 GO:0061695 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 map03022

Pfam Domains

Protein Families

TAFII28

Protein Analysis

226

Amino Acids

24.46

Weight (kDa)

5.68

Isoelectric Point (pI)

68.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TAFII28	PF04719	123 - 208	8.7e-33	hTAFII28-like protein conserved region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20000 AT4G20280
fragaria_vesca	FvH4_3g13420 FvH4_3g13420 FvH4_3g13420
malus_domestica	MD10G1223700.v1.1
prunus_persica	Prupe.4G121600_v2.0.a1
pyrus_communis	pycom10g18760
rosa_chinensis	RchiOBHm_Chr5g0022151
rosa_laevigata	RLG00000032648
rosa_multiflora	Rmu_co8448675.1_g000001 Rmu_sc0006966.1_g000013
rosa_roxburghii	Rroxscaffold_1G00056780
rosa_rugosa	Rorug05G0071600 Rorug05G0071700 Rorug05G0071800 Rorug05G0071900
rosa_samantha	Rh5AG159700 Rh5BG159200 Rh5CG173400 Rh5DG159200
rosa_wichuraiana	Rw5G014260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	643
AccB1I	GGYRCC	3 cut(s)	107, 232, 643
AccII	CGCG	4 cut(s)	92, 94, 96, 161
AciI	CCGC	8 cut(s)	32, 62, 90, 99, 159, 240, 255, 327
AclWI	GGATC	1 cut(s)	332
AcoI	YGGCCR	2 cut(s)	75, 351
AcuI	CTGAAG	1 cut(s)	105
AcyI	GRCGYC	1 cut(s)	233
AfaI	GTAC	2 cut(s)	404, 645
AfiI	CCNNNNNNNGG	2 cut(s)	118, 249
AgeI	ACCGGT	1 cut(s)	332
AgsI	TTSAA	2 cut(s)	628, 635
AjnI	CCWGG	1 cut(s)	139
AjuI	GAANNNNNNNTTGG	2 cut(s)	8, 40
AluBI	AGCT	4 cut(s)	170, 313, 422, 631
AluI	AGCT	4 cut(s)	170, 313, 422, 631
Alw21I	GWGCWC	1 cut(s)	315
Alw26I	GTCTC	1 cut(s)	539
AlwI	GGATC	1 cut(s)	332
AoxI	GGCC	6 cut(s)	75, 142, 251, 351, 584, 593
ApeKI	GCWGC	5 cut(s)	7, 170, 173, 221, 224
AsiGI	ACCGGT	1 cut(s)	332
Asp718I	GGTACC	1 cut(s)	643
AspLEI	GCGC	7 cut(s)	94, 96, 98, 163, 178, 235, 490
AspS9I	GGNCC	4 cut(s)	19, 143, 252, 584
AsuHPI	GGTGA	3 cut(s)	51, 347, 545
AsuII	TTCGAA	1 cut(s)	27
AvaII	GGWCC	1 cut(s)	19
BaeGI	GKGCMC	1 cut(s)	112
BalI	TGGCCA	1 cut(s)	353
BanI	GGYRCC	3 cut(s)	107, 232, 643
BanII	GRGCYC	1 cut(s)	315
BarI	GAAGNNNNNNTAC	2 cut(s)	627, 659
BbsI	GAAGAC	2 cut(s)	57, 300
Bbv12I	GWGCWC	1 cut(s)	315
BbvI	GCAGC	5 cut(s)	19, 157, 185, 208, 211
BccI	CCATC	1 cut(s)	172
BceAI	ACGGC	2 cut(s)	62, 246
BciT130I	CCWGG	1 cut(s)	141
BcoDI	GTCTC	1 cut(s)	539
BfaI	CTAG	3 cut(s)	113, 459, 663
BfmI	CTRYAG	1 cut(s)	171
BfoI	RGCGCY	2 cut(s)	179, 236
BglI	GCCNNNNNGGC	1 cut(s)	592
BisI	GCNGC	8 cut(s)	8, 33, 90, 99, 171, 174, 222, 225
BlsI	GCNGC	8 cut(s)	9, 34, 91, 100, 172, 175, 223, 226
Bme1390I	CCNGG	1 cut(s)	141
Bme18I	GGWCC	1 cut(s)	19
BmgT120I	GGNCC	4 cut(s)	19, 143, 252, 584
BmiI	GGNNCC	4 cut(s)	21, 109, 234, 645
BmrFI	CCNGG	1 cut(s)	141
BmsI	GCATC	2 cut(s)	348, 471
BpiI	GAAGAC	2 cut(s)	57, 300
Bpu14I	TTCGAA	1 cut(s)	27
BsaHI	GRCGYC	1 cut(s)	233
BsaJI	CCNNGG	1 cut(s)	15
BsaWI	WCCGGW	2 cut(s)	332, 672
Bsc4I	CCNNNNNNNGG	2 cut(s)	118, 249
Bse118I	RCCGGY	1 cut(s)	332
BseBI	CCWGG	1 cut(s)	141
BseDI	CCNNGG	1 cut(s)	15
BseGI	GGATG	2 cut(s)	277, 310
BseLI	CCNNNNNNNGG	2 cut(s)	118, 249
BseMII	CTCAG	1 cut(s)	396
BsePI	GCGCGC	2 cut(s)	92, 94
BseRI	GAGGAG	1 cut(s)	56
BseSI	GKGCMC	1 cut(s)	112
BseX3I	CGGCCG	1 cut(s)	75
BseXI	GCAGC	5 cut(s)	19, 157, 185, 208, 211
Bsh1236I	CGCG	4 cut(s)	92, 94, 96, 161
Bsh1285I	CGRYCG	1 cut(s)	78
BshFI	GGCC	6 cut(s)	77, 144, 253, 353, 586, 595
BshNI	GGYRCC	3 cut(s)	107, 232, 643
BshTI	ACCGGT	1 cut(s)	332
BsiEI	CGRYCG	1 cut(s)	78
BsiHKAI	GWGCWC	1 cut(s)	315
BsiSI	CCGG	3 cut(s)	74, 333, 673
BslI	CCNNNNNNNGG	2 cut(s)	118, 249
BsmAI	GTCTC	1 cut(s)	539
BsnI	GGCC	6 cut(s)	77, 144, 253, 353, 586, 595
Bsp119I	TTCGAA	1 cut(s)	27
Bsp1286I	GDGCHC	2 cut(s)	112, 315
Bsp143I	GATC	2 cut(s)	337, 653
BspACI	CCGC	8 cut(s)	32, 62, 90, 99, 159, 240, 255, 327
BspANI	GGCC	6 cut(s)	77, 144, 253, 353, 586, 595
BspCNI	CTCAG	1 cut(s)	395
BspFNI	CGCG	4 cut(s)	92, 94, 96, 161
BspLI	GGNNCC	4 cut(s)	21, 109, 234, 645
BspMAI	CTGCAG	1 cut(s)	175
BspPI	GGATC	1 cut(s)	332
BspQI	GCTCTTC	1 cut(s)	674
BspT104I	TTCGAA	1 cut(s)	27
BspT107I	GGYRCC	3 cut(s)	107, 232, 643
BsrFI	RCCGGY	1 cut(s)	332
BssAI	RCCGGY	1 cut(s)	332
BssECI	CCNNGG	1 cut(s)	15
BssHII	GCGCGC	2 cut(s)	92, 94
BssMI	GATC	2 cut(s)	337, 653
BssNI	GRCGYC	1 cut(s)	233
BssT1I	CCWWGG	1 cut(s)	15
Bst2UI	CCWGG	1 cut(s)	141
Bst4CI	ACNGT	1 cut(s)	660
Bst6I	CTCTTC	3 cut(s)	111, 135, 674
BstACI	GRCGYC	1 cut(s)	233
BstBI	TTCGAA	1 cut(s)	27
BstC8I	GCNNGC	4 cut(s)	94, 96, 255, 363
BstDEI	CTNAG	2 cut(s)	382, 605
BstENI	CCTNNNNNAGG	1 cut(s)	116
BstF5I	GGATG	2 cut(s)	277, 310
BstFNI	CGCG	4 cut(s)	92, 94, 96, 161
BstH2I	RGCGCY	2 cut(s)	179, 236
BstHHI	GCGC	7 cut(s)	94, 96, 98, 163, 178, 235, 490
BstKTI	GATC	2 cut(s)	340, 656
BstMAI	GTCTC	1 cut(s)	539
BstMBI	GATC	2 cut(s)	337, 653
BstMCI	CGRYCG	1 cut(s)	78
BstNI	CCWGG	1 cut(s)	141
BstSCI	CCNGG	1 cut(s)	139
BstSFI	CTRYAG	1 cut(s)	171
BstSLI	GKGCMC	1 cut(s)	112
BstUI	CGCG	4 cut(s)	92, 94, 96, 161
BstV1I	GCAGC	5 cut(s)	19, 157, 185, 208, 211
BstV2I	GAAGAC	2 cut(s)	57, 300
BstZI	CGGCCG	1 cut(s)	75
BsuRI	GGCC	6 cut(s)	77, 144, 253, 353, 586, 595
BtsCI	GGATG	2 cut(s)	277, 310
BtsIMutI	CAGTG	1 cut(s)	472
Cac8I	GCNNGC	4 cut(s)	94, 96, 255, 363
CfoI	GCGC	7 cut(s)	94, 96, 98, 163, 178, 235, 490
Cfr10I	RCCGGY	1 cut(s)	332
Cfr13I	GGNCC	4 cut(s)	19, 143, 252, 584
Csp6I	GTAC	2 cut(s)	403, 644
CspAI	ACCGGT	1 cut(s)	332
CviAII	CATG	3 cut(s)	301, 442, 597
CviQI	GTAC	2 cut(s)	403, 644
DdeI	CTNAG	2 cut(s)	382, 605
DinI	GGCGCC	1 cut(s)	234
DpnI	GATC	2 cut(s)	339, 655
DpnII	GATC	2 cut(s)	337, 653
EaeI	YGGCCR	2 cut(s)	75, 351
EagI	CGGCCG	1 cut(s)	75
Eam1104I	CTCTTC	3 cut(s)	111, 135, 674
EarI	CTCTTC	3 cut(s)	111, 135, 674
EciI	GGCGGA	2 cut(s)	229, 316
Ecl136II	GAGCTC	1 cut(s)	313
EclXI	CGGCCG	1 cut(s)	75
Eco130I	CCWWGG	1 cut(s)	15
Eco24I	GRGCYC	1 cut(s)	315
Eco47I	GGWCC	1 cut(s)	19
Eco52I	CGGCCG	1 cut(s)	75
Eco53kI	GAGCTC	1 cut(s)	313
Eco57I	CTGAAG	1 cut(s)	105
EcoICRI	GAGCTC	1 cut(s)	313
EcoNI	CCTNNNNNAGG	1 cut(s)	116
EcoO109I	RGGNCCY	1 cut(s)	19
EcoRII	CCWGG	1 cut(s)	139
EcoT14I	CCWWGG	1 cut(s)	15
EcoT38I	GRGCYC	1 cut(s)	315
EgeI	GGCGCC	1 cut(s)	234
EheI	GGCGCC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	15
FaeI	CATG	3 cut(s)	304, 445, 600
FaiI	YATR	4 cut(s)	302, 443, 598, 616
FatI	CATG	3 cut(s)	300, 441, 596
FauI	CCCGC	1 cut(s)	262
Fnu4HI	GCNGC	8 cut(s)	8, 33, 90, 99, 171, 174, 222, 225
FokI	GGATG	2 cut(s)	284, 317
FriOI	GRGCYC	1 cut(s)	315
Fsp4HI	GCNGC	8 cut(s)	8, 33, 90, 99, 171, 174, 222, 225
FspBI	CTAG	3 cut(s)	113, 459, 663
GlaI	GCGC	7 cut(s)	93, 95, 97, 162, 177, 234, 489
GluI	GCNGC	8 cut(s)	8, 33, 90, 99, 171, 174, 222, 225
HaeII	RGCGCY	2 cut(s)	179, 236
HaeIII	GGCC	6 cut(s)	77, 144, 253, 353, 586, 595
HapII	CCGG	3 cut(s)	74, 333, 673
HhaI	GCGC	7 cut(s)	94, 96, 98, 163, 178, 235, 490
Hin1I	GRCGYC	1 cut(s)	233
Hin1II	CATG	3 cut(s)	304, 445, 600
Hin6I	GCGC	7 cut(s)	92, 94, 96, 161, 176, 233, 488
HinP1I	GCGC	7 cut(s)	92, 94, 96, 161, 176, 233, 488
HincII	GTYRAC	1 cut(s)	456
HindII	GTYRAC	1 cut(s)	456
HindIII	AAGCTT	1 cut(s)	629
HinfI	GANTC	4 cut(s)	56, 68, 407, 578
HpaI	GTTAAC	1 cut(s)	456
HpaII	CCGG	3 cut(s)	74, 333, 673
HphI	GGTGA	3 cut(s)	51, 347, 545
Hpy166II	GTNNAC	2 cut(s)	456, 536
Hpy188I	TCNGA	4 cut(s)	317, 385, 432, 568
Hpy188III	TCNNGA	2 cut(s)	262, 341
Hpy8I	GTNNAC	2 cut(s)	456, 536
Hpy99I	CGWCG	5 cut(s)	83, 232, 281, 284, 293
HpyAV	CCTTC	3 cut(s)	615, 629, 634
HpyCH4III	ACNGT	1 cut(s)	660
HpyCH4V	TGCA	2 cut(s)	173, 361
HpyF3I	CTNAG	2 cut(s)	382, 605
Hsp92I	GRCGYC	1 cut(s)	233
Hsp92II	CATG	3 cut(s)	304, 445, 600
HspAI	GCGC	7 cut(s)	92, 94, 96, 161, 176, 233, 488
KasI	GGCGCC	1 cut(s)	232
KpnI	GGTACC	1 cut(s)	647
KspAI	GTTAAC	1 cut(s)	456
Kzo9I	GATC	2 cut(s)	337, 653
LguI	GCTCTTC	1 cut(s)	674
LmnI	GCTCC	1 cut(s)	43
Lsp1109I	GCAGC	5 cut(s)	19, 157, 185, 208, 211
LweI	GCATC	2 cut(s)	348, 471
MaeI	CTAG	3 cut(s)	113, 459, 663
MaeIII	GTNAC	1 cut(s)	193
MalI	GATC	2 cut(s)	339, 655
MauBI	CGCGCGCG	1 cut(s)	92
MboI	GATC	2 cut(s)	337, 653
MboII	GAAGA	6 cut(s)	62, 122, 128, 144, 305, 661
MhlI	GDGCHC	2 cut(s)	112, 315
MlsI	TGGCCA	1 cut(s)	353
MluCI	AATT	1 cut(s)	377
MluNI	TGGCCA	1 cut(s)	353
Mly113I	GGCGCC	1 cut(s)	233
MlyI	GAGTC	3 cut(s)	50, 416, 587
MnlI	CCTC	8 cut(s)	34, 112, 138, 166, 247, 253, 379, 564
Mox20I	TGGCCA	1 cut(s)	353
MscI	TGGCCA	1 cut(s)	353
MseI	TTAA	1 cut(s)	455
Msp20I	TGGCCA	1 cut(s)	353
MspA1I	CMGCKG	2 cut(s)	170, 257
MspI	CCGG	3 cut(s)	74, 333, 673
MspR9I	CCNGG	1 cut(s)	141
MvaI	CCWGG	1 cut(s)	141
MvnI	CGCG	4 cut(s)	92, 94, 96, 161
NarI	GGCGCC	1 cut(s)	233
NdeII	GATC	2 cut(s)	337, 653
NlaIII	CATG	3 cut(s)	304, 445, 600
NlaIV	GGNNCC	4 cut(s)	21, 109, 234, 645
NmeAIII	GCCGAG	1 cut(s)	229
NspV	TTCGAA	1 cut(s)	27
PauI	GCGCGC	2 cut(s)	92, 94
PciSI	GCTCTTC	1 cut(s)	674
PcsI	WCGNNNNNNNCGW	2 cut(s)	285, 288
PfeI	GAWTC	1 cut(s)	68
PinAI	ACCGGT	1 cut(s)	332
PkrI	GCNGC	8 cut(s)	9, 34, 91, 100, 172, 175, 223, 226
PleI	GAGTC	3 cut(s)	50, 415, 586
PluTI	GGCGCC	1 cut(s)	236
PpsI	GAGTC	3 cut(s)	50, 415, 586
PpuMI	RGGWCCY	1 cut(s)	19
Psp124BI	GAGCTC	1 cut(s)	315
Psp5II	RGGWCCY	1 cut(s)	19
Psp6I	CCWGG	1 cut(s)	139
PspGI	CCWGG	1 cut(s)	139
PspN4I	GGNNCC	4 cut(s)	21, 109, 234, 645
PspPI	GGNCC	4 cut(s)	19, 143, 252, 584
PspPPI	RGGWCCY	1 cut(s)	19
PstI	CTGCAG	1 cut(s)	175
PteI	GCGCGC	2 cut(s)	92, 94
PvuII	CAGCTG	1 cut(s)	170
RsaI	GTAC	2 cut(s)	404, 645
RsaNI	GTAC	2 cut(s)	403, 644
SacI	GAGCTC	1 cut(s)	315
SapI	GCTCTTC	1 cut(s)	674
SaqAI	TTAA	1 cut(s)	455
SatI	GCNGC	8 cut(s)	8, 33, 90, 99, 171, 174, 222, 225
Sau3AI	GATC	2 cut(s)	337, 653
Sau96I	GGNCC	4 cut(s)	19, 143, 252, 584
SchI	GAGTC	3 cut(s)	50, 416, 587
ScrFI	CCNGG	1 cut(s)	141
SduI	GDGCHC	2 cut(s)	112, 315
SetI	ASST	9 cut(s)	172, 315, 404, 424, 452, 606, 626, 633, 645
SfaNI	GCATC	2 cut(s)	348, 471
SfcI	CTRYAG	1 cut(s)	171
SfiI	GGCCNNNNNGGCC	1 cut(s)	592
SfoI	GGCGCC	1 cut(s)	234
SfuI	TTCGAA	1 cut(s)	27
SgrAI	CRCCGGYG	1 cut(s)	332
SinI	GGWCC	1 cut(s)	19
Sse9I	AATT	1 cut(s)	377
SsiI	CCGC	8 cut(s)	32, 62, 90, 99, 159, 240, 255, 327
SspDI	GGCGCC	1 cut(s)	232
SspI	AATATT	1 cut(s)	499
SspMI	CTAG	3 cut(s)	113, 459, 663
SstI	GAGCTC	1 cut(s)	315
StyD4I	CCNGG	1 cut(s)	139
StyI	CCWWGG	1 cut(s)	15
TaaI	ACNGT	1 cut(s)	660
TaqI	TCGA	3 cut(s)	27, 39, 309
TasI	AATT	1 cut(s)	377
TauI	GCSGC	3 cut(s)	35, 92, 101
TfiI	GAWTC	1 cut(s)	68
Tru1I	TTAA	1 cut(s)	455
Tru9I	TTAA	1 cut(s)	455
TscAI	CASTG	1 cut(s)	472
TseI	GCWGC	5 cut(s)	7, 170, 173, 221, 224
TspDTI	ATGAA	3 cut(s)	17, 458, 509
TspRI	CASTG	1 cut(s)	472
VpaK11BI	GGWCC	1 cut(s)	19
XagI	CCTNNNNNAGG	1 cut(s)	116
XspI	CTAG	3 cut(s)	113, 459, 663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.