Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0011167

Physical Location & Seq

Genomic Coordinates Forward (+)

32795856 .. 32798906

Gene Structure 3051 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1231200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGAATAGTGAGCTCCGATCCAGCAAAGCCAAGAGGACTGCCAAACCCTCCTCTCCTTCTTCCACCAACTCCCCCCATGCCCGCCGCCGCCGCCAAATCCTAACCCAATTCCTCTCAATGGCGCGAAAATCCTCGCTCCTCAAGCAATCCCTCGCCACCATCGTCGGCGTCCTCGTCCTCTACGCCTTCCTCAGCACCTTCCTCACTCCCGCCGCCACTTCCAAGCTCGAGAACGCCTTCCCCTCCTTCTCCTCCGCCGCTTCCAATTCGATCTCCAACGAGTTTGTGTCCCGGGAAAATCAGCTTAATTTTCCCGGAAAGCCCGTCAAAGTGTATCTCTACGATCTCCCGAAGCGGTTCACTTACGGAGTCATCAAGCATCACTCGTTGGCCCGCGGCGGCCGGCCTGACGACGACGTTTTGAAGCTCAAGTACCCCGGTCACCAGCACATGGGCGAGTGGTACCTGTTCCAGGACCTGCTCAAACCCGATTCGGAACGGGTCGGGTCTCCAGTCGAGAATATATTGGATCCGGAAGAAGCAGACTTGTTCTACGTGCCGTTCTTCTCGTCATTGAGCCTGATTGTTAACCAGGCCCGACCGGCCTCCGGGTCGGAGAAGCCGTTGTACAGCGACGAGGAGAATCAGGTGGCGTTGATTGAGTGGCTGGAGGCGCAGGAGTACTGGAAGAGGAACAATGGCCGAGACCATGTGATCATGGCTTCGGACCCCAATGCTCTGTACAAAGTGATTGACAGAGTGAAAAACTGTGTGTTGCTTGTTTGCGATTTCGGGCGGTTGAAGGAGGACCAAGGATCGCTTGTGAAGGACGTGATCGTGCCTTACTCTCACCGCATCAACACTTACACCGGGGATATCAGCGTCGAAAATCGCAACACCTTGTTGTTCTTCATGGGCAACCGCTTCCGAAAAGAGGGCGGAAAGATACGTGATTTACTTTTCCAATTACTTGAAAATGAAGAGGATGTTATAGTAAAGCATGGAACACAATCCAGAGTGAGTCGACGGGCTGCTACACAGGGGATGCATATGTCGAAGTTCTGTTTGAATCCTGCTGGTGATACTCCATCAGCCTGCCGACTTTTTGATTCTATAGTTAGCATGTGCGTGCCTGTGATAGTCAGCGATAGCATTGAGCTGCCCTTCGAAGATGTGATAGACTACAGAAGGATAGCAATATTTGTAGAATCCAATGCAGCTTTAAAGCCAGGATTCTTAGTCTCAATGCTGAGAGGAATCCCCACTGAGAGAATTCTGGAATACCAGACAGAGCTGAATGAGGTGAAGCATTATTTTCAATATGGAGAACCAAATGGAACAGTAAATGAAATCTGGCGCCAGATCGCACAGAAGCTCCCTTTTATCAAATTGACAATCAACCGCAACAGGCGACTTGTCAAGAGGGATTCGAATGTGCGAGACTGCTCTTGTCTCTGCTCAAACCAGACCGGGATTATAACTAGCCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010393 GO:0010410 GO:0010413 GO:0010417 GO:0012505 GO:0015020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016763 GO:0017144 GO:0031221 GO:0031410 GO:0031982 GO:0031984 GO:0033692 GO:0034637 GO:0034645 GO:0035884 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045488 GO:0045489 GO:0045491 GO:0045492 GO:0047517 GO:0070589 GO:0070592 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0080116 GO:0097708 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

497

Amino Acids

56.27

Weight (kDa)

9.0

Isoelectric Point (pI)

48.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	107 - 416	7.7e-74	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35100
fragaria_vesca	FvH4_3g12450 FvH4_3g12450
malus_domestica	MD05G1250200.v1.1 MD10G1231200.v1.1
prunus_persica	Prupe.4G113400_v2.0.a1
pyrus_communis	pycom03g23690 pycom05g22820 pycom10g19440
rosa_chinensis	RchiOBHm_Chr5g0020381
rosa_laevigata	RLG00000032518
rosa_multiflora	Rmu_sc0002087.1_g000001 Rmu_sc0007157.1_g000001
rosa_roxburghii	Rroxscaffold_1G00057880
rosa_rugosa	Rorug05G0058900
rosa_samantha	Rh5AG149100 Rh5BG148800 Rh5CG160400 Rh5DG147500
rosa_wichuraiana	Rw5G013220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1478
Acc36I	ACCTGC	1 cut(s)	486
Acc65I	GGTACC	1 cut(s)	462
AccB1I	GGYRCC	2 cut(s)	462, 1356
AccB7I	CCANNNNNTGG	1 cut(s)	451
AccI	GTMKAC	1 cut(s)	1024
AccII	CGCG	2 cut(s)	124, 396
AccIII	TCCGGA	1 cut(s)	532
AclWI	GGATC	4 cut(s)	12, 524, 537, 823
AcoI	YGGCCR	2 cut(s)	400, 700
AcsI	RAATTY	1 cut(s)	1272
AcyI	GRCGYC	2 cut(s)	168, 1357
AfaI	GTAC	5 cut(s)	434, 464, 629, 683, 743
AfiI	CCNNNNNNNGG	5 cut(s)	118, 451, 472, 608, 934
AgsI	TTSAA	5 cut(s)	424, 802, 974, 1069, 1319
AjiI	CACGTC	1 cut(s)	832
AjnI	CCWGG	3 cut(s)	471, 591, 1228
AluBI	AGCT	8 cut(s)	13, 226, 304, 427, 1159, 1220, 1294, 1375
AluI	AGCT	8 cut(s)	13, 226, 304, 427, 1159, 1220, 1294, 1375
Alw21I	GWGCWC	1 cut(s)	15
Alw26I	GTCTC	5 cut(s)	513, 699, 1246, 1434, 1457
AlwI	GGATC	4 cut(s)	12, 524, 537, 823
AlwNI	CAGNNNCTG	1 cut(s)	478
Ama87I	CYCGRG	2 cut(s)	227, 291
Aor13HI	TCCGGA	1 cut(s)	532
AoxI	GGCC	6 cut(s)	390, 400, 404, 594, 603, 700
ApeKI	GCWGC	3 cut(s)	1031, 1159, 1217
ApoI	RAATTY	1 cut(s)	1272
Asp700I	GAANNNNTTC	2 cut(s)	236, 356
Asp718I	GGTACC	1 cut(s)	462
AspLEI	GCGC	3 cut(s)	124, 676, 1359
AspS9I	GGNCC	5 cut(s)	391, 475, 595, 727, 808
AsuC2I	CCSGG	7 cut(s)	292, 293, 315, 438, 610, 871, 1471
AsuHPI	GGTGA	4 cut(s)	434, 842, 1091, 1315
AsuII	TTCGAA	2 cut(s)	1167, 1430
AvaI	CYCGRG	2 cut(s)	227, 291
AvaII	GGWCC	3 cut(s)	475, 727, 808
BamHI	GGATCC	1 cut(s)	529
BanI	GGYRCC	2 cut(s)	462, 1356
BanII	GRGCYC	1 cut(s)	15
BarI	GAAGNNNNNNTAC	4 cut(s)	416, 448, 611, 643
Bbv12I	GWGCWC	1 cut(s)	15
BbvCI	CCTCAGC	1 cut(s)	191
BbvI	GCAGC	3 cut(s)	1018, 1146, 1229
BccI	CCATC	2 cut(s)	167, 1096
BceAI	ACGGC	2 cut(s)	544, 607
BciT130I	CCWGG	3 cut(s)	473, 593, 1230
BclI	TGATCA	1 cut(s)	714
BcnI	CCSGG	7 cut(s)	292, 293, 315, 438, 610, 871, 1471
BcoDI	GTCTC	5 cut(s)	513, 699, 1246, 1434, 1457
BfaI	CTAG	1 cut(s)	1482
BfmI	CTRYAG	2 cut(s)	1113, 1183
BfoI	RGCGCY	1 cut(s)	1360
BfuAI	ACCTGC	1 cut(s)	486
BglI	GCCNNNNNGGC	1 cut(s)	602
BmcAI	AGTACT	1 cut(s)	683
Bme18I	GGWCC	3 cut(s)	475, 727, 808
BmeT110I	CYCGRG	2 cut(s)	227, 291
BmgBI	CACGTC	1 cut(s)	832
BmgT120I	GGNCC	5 cut(s)	391, 475, 595, 727, 808
BmiI	GGNNCC	4 cut(s)	464, 531, 729, 1358
BmsI	GCATC	3 cut(s)	388, 864, 1035
BpmI	CTGGAG	2 cut(s)	495, 689
Bpu10I	CCTNAGC	1 cut(s)	191
Bpu14I	TTCGAA	2 cut(s)	1167, 1430
BpuEI	CTTGAG	2 cut(s)	125, 413
BpuMI	CCSGG	7 cut(s)	292, 293, 315, 438, 610, 871, 1471
BsaAI	YACGTR	2 cut(s)	556, 950
BsaHI	GRCGYC	2 cut(s)	168, 1357
BsaI	GGTCTC	2 cut(s)	513, 699
BsaJI	CCNNGG	5 cut(s)	291, 394, 436, 811, 870
BsaWI	WCCGGW	1 cut(s)	532
BsaXI	ACNNNNNCTCC	4 cut(s)	608, 638, 1359, 1389
Bsc4I	CCNNNNNNNGG	5 cut(s)	118, 451, 472, 608, 934
Bse118I	RCCGGY	2 cut(s)	402, 601
Bse1I	ACTGG	2 cut(s)	512, 689
BseAI	TCCGGA	1 cut(s)	532
BseBI	CCWGG	3 cut(s)	473, 593, 1230
BseDI	CCNNGG	5 cut(s)	291, 394, 436, 811, 870
BseGI	GGATG	2 cut(s)	991, 1050
BseLI	CCNNNNNNNGG	5 cut(s)	118, 451, 472, 608, 934
BseMII	CTCAG	3 cut(s)	205, 1241, 1257
BseNI	ACTGG	2 cut(s)	512, 689
BseRI	GAGGAG	4 cut(s)	40, 128, 241, 653
BseX3I	CGGCCG	1 cut(s)	400
BseXI	GCAGC	3 cut(s)	1018, 1146, 1229
Bsh1236I	CGCG	2 cut(s)	124, 396
Bsh1285I	CGRYCG	2 cut(s)	403, 602
BshFI	GGCC	6 cut(s)	392, 402, 406, 596, 605, 702
BshNI	GGYRCC	2 cut(s)	462, 1356
BsiEI	CGRYCG	2 cut(s)	403, 602
BsiHKAI	GWGCWC	1 cut(s)	15
BsiHKCI	CYCGRG	2 cut(s)	227, 291
BsiSI	CCGG	9 cut(s)	292, 315, 403, 438, 533, 602, 609, 870, 1470
BslFI	GGGAC	1 cut(s)	274
BslI	CCNNNNNNNGG	5 cut(s)	118, 451, 472, 608, 934
BsmAI	GTCTC	5 cut(s)	513, 699, 1246, 1434, 1457
BsmFI	GGGAC	1 cut(s)	274
BsnI	GGCC	6 cut(s)	392, 402, 406, 596, 605, 702
Bso31I	GGTCTC	2 cut(s)	513, 699
BsoBI	CYCGRG	2 cut(s)	227, 291
Bsp119I	TTCGAA	2 cut(s)	1167, 1430
Bsp1286I	GDGCHC	1 cut(s)	15
Bsp13I	TCCGGA	1 cut(s)	532
Bsp1407I	TGTACA	2 cut(s)	627, 741
Bsp143I	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
BspANI	GGCC	6 cut(s)	392, 402, 406, 596, 605, 702
BspCNI	CTCAG	3 cut(s)	204, 1242, 1258
BspEI	TCCGGA	1 cut(s)	532
BspFNI	CGCG	2 cut(s)	124, 396
BspLI	GGNNCC	4 cut(s)	464, 531, 729, 1358
BspMI	ACCTGC	1 cut(s)	486
BspPI	GGATC	4 cut(s)	12, 524, 537, 823
BspT104I	TTCGAA	2 cut(s)	1167, 1430
BspT107I	GGYRCC	2 cut(s)	462, 1356
BspTNI	GGTCTC	2 cut(s)	513, 699
BsrFI	RCCGGY	2 cut(s)	402, 601
BsrGI	TGTACA	2 cut(s)	627, 741
BsrI	ACTGG	2 cut(s)	512, 689
BssAI	RCCGGY	2 cut(s)	402, 601
BssECI	CCNNGG	5 cut(s)	291, 394, 436, 811, 870
BssMI	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
BssNI	GRCGYC	2 cut(s)	168, 1357
BssT1I	CCWWGG	1 cut(s)	811
Bst2UI	CCWGG	3 cut(s)	473, 593, 1230
Bst4CI	ACNGT	2 cut(s)	770, 1342
Bst6I	CTCTTC	2 cut(s)	683, 975
BstACI	GRCGYC	2 cut(s)	168, 1357
BstAUI	TGTACA	2 cut(s)	627, 741
BstBAI	YACGTR	2 cut(s)	556, 950
BstBI	TTCGAA	2 cut(s)	1167, 1430
BstC8I	GCNNGC	5 cut(s)	82, 394, 404, 1096, 1130
BstDEI	CTNAG	4 cut(s)	191, 1237, 1250, 1266
BstDSI	CCRYGG	1 cut(s)	394
BstEII	GGTNACC	1 cut(s)	440
BstENI	CCTNNNNNAGG	1 cut(s)	470
BstF5I	GGATG	2 cut(s)	991, 1050
BstFNI	CGCG	2 cut(s)	124, 396
BstH2I	RGCGCY	1 cut(s)	1360
BstHHI	GCGC	3 cut(s)	124, 676, 1359
BstKTI	GATC	8 cut(s)	20, 273, 346, 532, 717, 818, 837, 1365
BstMAI	GTCTC	5 cut(s)	513, 699, 1246, 1434, 1457
BstMBI	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
BstMCI	CGRYCG	2 cut(s)	403, 602
BstMWI	GCNNNNNNNGC	4 cut(s)	90, 142, 602, 673
BstNI	CCWGG	3 cut(s)	473, 593, 1230
BstNSI	RCATGY	1 cut(s)	1126
BstPI	GGTNACC	1 cut(s)	440
BstSFI	CTRYAG	2 cut(s)	1113, 1183
BstUI	CGCG	2 cut(s)	124, 396
BstV1I	GCAGC	3 cut(s)	1018, 1146, 1229
BstX2I	RGATCY	1 cut(s)	529
BstYI	RGATCY	1 cut(s)	529
BstZI	CGGCCG	1 cut(s)	400
BsuRI	GGCC	6 cut(s)	392, 402, 406, 596, 605, 702
BtgI	CCRYGG	1 cut(s)	394
BtrI	CACGTC	1 cut(s)	832
BtsCI	GGATG	2 cut(s)	991, 1050
BtsIMutI	CAGTG	1 cut(s)	1263
BveI	ACCTGC	1 cut(s)	486
Cac8I	GCNNGC	5 cut(s)	82, 394, 404, 1096, 1130
CaiI	CAGNNNCTG	1 cut(s)	478
CfoI	GCGC	3 cut(s)	124, 676, 1359
Cfr10I	RCCGGY	2 cut(s)	402, 601
Cfr13I	GGNCC	5 cut(s)	391, 475, 595, 727, 808
Cfr42I	CCGCGG	1 cut(s)	397
Cfr9I	CCCGGG	1 cut(s)	291
CseI	GACGC	2 cut(s)	157, 871
Csp6I	GTAC	5 cut(s)	433, 463, 628, 682, 742
CviAII	CATG	7 cut(s)	77, 451, 710, 718, 913, 1001, 1123
CviQI	GTAC	5 cut(s)	433, 463, 628, 682, 742
DdeI	CTNAG	4 cut(s)	191, 1237, 1250, 1266
DinI	GGCGCC	1 cut(s)	1358
DpnI	GATC	8 cut(s)	19, 272, 345, 531, 716, 817, 836, 1364
DpnII	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
DraI	TTTAAA	1 cut(s)	1224
EaeI	YGGCCR	2 cut(s)	400, 700
EagI	CGGCCG	1 cut(s)	400
Eam1104I	CTCTTC	2 cut(s)	683, 975
EarI	CTCTTC	2 cut(s)	683, 975
EciI	GGCGGA	2 cut(s)	244, 954
Ecl136II	GAGCTC	1 cut(s)	13
EclXI	CGGCCG	1 cut(s)	400
Eco130I	CCWWGG	1 cut(s)	811
Eco24I	GRGCYC	1 cut(s)	15
Eco31I	GGTCTC	2 cut(s)	513, 699
Eco32I	GATATC	1 cut(s)	877
Eco47I	GGWCC	3 cut(s)	475, 727, 808
Eco52I	CGGCCG	1 cut(s)	400
Eco53kI	GAGCTC	1 cut(s)	13
Eco88I	CYCGRG	2 cut(s)	227, 291
Eco91I	GGTNACC	1 cut(s)	440
EcoICRI	GAGCTC	1 cut(s)	13
EcoNI	CCTNNNNNAGG	1 cut(s)	470
EcoO109I	RGGNCCY	1 cut(s)	475
EcoO65I	GGTNACC	1 cut(s)	440
EcoRI	GAATTC	1 cut(s)	1272
EcoRII	CCWGG	3 cut(s)	471, 591, 1228
EcoRV	GATATC	1 cut(s)	877
EcoT14I	CCWWGG	1 cut(s)	811
EcoT22I	ATGCAT	1 cut(s)	1050
EcoT38I	GRGCYC	1 cut(s)	15
EgeI	GGCGCC	1 cut(s)	1358
EheI	GGCGCC	1 cut(s)	1358
ErhI	CCWWGG	1 cut(s)	811
FaeI	CATG	7 cut(s)	80, 454, 713, 721, 916, 1004, 1126
FalI	AAGNNNNNCTT	2 cut(s)	804, 836
FaqI	GGGAC	1 cut(s)	274
FatI	CATG	7 cut(s)	76, 450, 709, 717, 912, 1000, 1122
FauI	CCCGC	3 cut(s)	89, 217, 401
FauNDI	CATATG	1 cut(s)	1050
FbaI	TGATCA	1 cut(s)	714
FblI	GTMKAC	1 cut(s)	1024
FokI	GGATG	2 cut(s)	998, 1057
FriOI	GRGCYC	1 cut(s)	15
FseI	GGCCGGCC	1 cut(s)	406
FspBI	CTAG	1 cut(s)	1482
GlaI	GCGC	3 cut(s)	123, 675, 1358
GsuI	CTGGAG	2 cut(s)	495, 689
HaeII	RGCGCY	1 cut(s)	1360
HaeIII	GGCC	6 cut(s)	392, 402, 406, 596, 605, 702
HapII	CCGG	9 cut(s)	292, 315, 403, 438, 533, 602, 609, 870, 1470
HgaI	GACGC	2 cut(s)	157, 871
HhaI	GCGC	3 cut(s)	124, 676, 1359
Hin1I	GRCGYC	2 cut(s)	168, 1357
Hin1II	CATG	7 cut(s)	80, 454, 713, 721, 916, 1004, 1126
Hin6I	GCGC	3 cut(s)	122, 674, 1357
HinP1I	GCGC	3 cut(s)	122, 674, 1357
HincII	GTYRAC	2 cut(s)	589, 1025
HindII	GTYRAC	2 cut(s)	589, 1025
HpaI	GTTAAC	1 cut(s)	589
HpaII	CCGG	9 cut(s)	292, 315, 403, 438, 533, 602, 609, 870, 1470
HphI	GGTGA	4 cut(s)	434, 842, 1091, 1315
Hpy166II	GTNNAC	3 cut(s)	360, 589, 1025
Hpy188I	TCNGA	5 cut(s)	17, 496, 616, 727, 929
Hpy188III	TCNNGA	7 cut(s)	229, 349, 517, 533, 1014, 1277, 1420
Hpy8I	GTNNAC	3 cut(s)	360, 589, 1025
Hpy99I	CGWCG	6 cut(s)	167, 416, 419, 638, 887, 1029
HpyAV	CCTTC	9 cut(s)	66, 196, 208, 247, 256, 796, 820, 1174, 1182
HpyCH4III	ACNGT	2 cut(s)	770, 1342
HpyCH4IV	ACGT	4 cut(s)	417, 555, 831, 949
HpyCH4V	TGCA	2 cut(s)	1048, 1217
HpyF10VI	GCNNNNNNNGC	4 cut(s)	90, 142, 602, 673
HpyF3I	CTNAG	4 cut(s)	191, 1237, 1250, 1266
HpySE526I	ACGT	4 cut(s)	417, 555, 831, 949
Hsp92I	GRCGYC	2 cut(s)	168, 1357
Hsp92II	CATG	7 cut(s)	80, 454, 713, 721, 916, 1004, 1126
HspAI	GCGC	3 cut(s)	122, 674, 1357
KasI	GGCGCC	1 cut(s)	1356
Kpn2I	TCCGGA	1 cut(s)	532
KpnI	GGTACC	1 cut(s)	466
KroI	GCCGGC	1 cut(s)	402
KroNI	GCCGGC	1 cut(s)	404
Ksp22I	TGATCA	1 cut(s)	714
KspAI	GTTAAC	1 cut(s)	589
KspI	CCGCGG	1 cut(s)	397
Kzo9I	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
LmnI	GCTCC	3 cut(s)	18, 141, 1380
Lsp1109I	GCAGC	3 cut(s)	1018, 1146, 1229
LweI	GCATC	3 cut(s)	388, 864, 1035
MaeI	CTAG	1 cut(s)	1482
MaeII	ACGT	4 cut(s)	417, 555, 831, 949
MaeIII	GTNAC	1 cut(s)	440
MalI	GATC	8 cut(s)	19, 272, 345, 531, 716, 817, 836, 1364
MboI	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
MboII	GAAGA	7 cut(s)	51, 548, 556, 700, 901, 992, 1181
MflI	RGATCY	1 cut(s)	529
MhlI	GDGCHC	1 cut(s)	15
MluCI	AATT	6 cut(s)	107, 265, 307, 965, 1272, 1388
Mly113I	GGCGCC	1 cut(s)	1357
MlyI	GAGTC	2 cut(s)	378, 1030
MmeI	TCCRAC	2 cut(s)	300, 594
Mph1103I	ATGCAT	1 cut(s)	1050
MroI	TCCGGA	1 cut(s)	532
MroNI	GCCGGC	1 cut(s)	402
MroXI	GAANNNNTTC	2 cut(s)	236, 356
MseI	TTAA	3 cut(s)	306, 588, 1223
MslI	CAYNNNNRTG	1 cut(s)	1127
MspA1I	CMGCKG	1 cut(s)	396
MspI	CCGG	9 cut(s)	292, 315, 403, 438, 533, 602, 609, 870, 1470
MvaI	CCWGG	3 cut(s)	473, 593, 1230
MvnI	CGCG	2 cut(s)	124, 396
MwoI	GCNNNNNNNGC	4 cut(s)	90, 142, 602, 673
NaeI	GCCGGC	1 cut(s)	404
NarI	GGCGCC	1 cut(s)	1357
NciI	CCSGG	7 cut(s)	292, 293, 315, 438, 610, 871, 1471
NdeI	CATATG	1 cut(s)	1050
NdeII	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
NgoMIV	GCCGGC	1 cut(s)	402
NlaIII	CATG	7 cut(s)	80, 454, 713, 721, 916, 1004, 1126
NlaIV	GGNNCC	4 cut(s)	464, 531, 729, 1358
NmeAIII	GCCGAG	1 cut(s)	728
NmuCI	GTSAC	1 cut(s)	440
NsiI	ATGCAT	1 cut(s)	1050
NspI	RCATGY	1 cut(s)	1126
NspV	TTCGAA	2 cut(s)	1167, 1430
PaeR7I	CTCGAG	1 cut(s)	227
PcsI	WCGNNNNNNNCGW	3 cut(s)	159, 171, 620
PdiI	GCCGGC	1 cut(s)	404
PdmI	GAANNNNTTC	2 cut(s)	236, 356
PfeI	GAWTC	8 cut(s)	491, 643, 1069, 1109, 1208, 1233, 1257, 1427
PflMI	CCANNNNNTGG	1 cut(s)	451
PfoI	TCCNGGA	2 cut(s)	313, 471
PleI	GAGTC	2 cut(s)	377, 1029
PluTI	GGCGCC	1 cut(s)	1360
PpsI	GAGTC	2 cut(s)	377, 1029
Ppu21I	YACGTR	2 cut(s)	556, 950
PpuMI	RGGWCCY	1 cut(s)	475
PsiI	TTATAA	1 cut(s)	1478
Psp124BI	GAGCTC	1 cut(s)	15
Psp5II	RGGWCCY	1 cut(s)	475
Psp6I	CCWGG	3 cut(s)	471, 591, 1228
PspEI	GGTNACC	1 cut(s)	440
PspGI	CCWGG	3 cut(s)	471, 591, 1228
PspN4I	GGNNCC	4 cut(s)	464, 531, 729, 1358
PspPI	GGNCC	5 cut(s)	391, 475, 595, 727, 808
PspPPI	RGGWCCY	1 cut(s)	475
PstNI	CAGNNNCTG	1 cut(s)	478
PsuI	RGATCY	1 cut(s)	529
RigI	GGCCGGCC	1 cut(s)	406
RsaI	GTAC	5 cut(s)	434, 464, 629, 683, 743
RsaNI	GTAC	5 cut(s)	433, 463, 628, 682, 742
RseI	CAYNNNNRTG	1 cut(s)	1127
SacI	GAGCTC	1 cut(s)	15
SacII	CCGCGG	1 cut(s)	397
SalI	GTCGAC	1 cut(s)	1023
SaqAI	TTAA	3 cut(s)	306, 588, 1223
Sau3AI	GATC	8 cut(s)	17, 270, 343, 529, 714, 815, 834, 1362
Sau96I	GGNCC	5 cut(s)	391, 475, 595, 727, 808
ScaI	AGTACT	1 cut(s)	683
SchI	GAGTC	2 cut(s)	378, 1030
SduI	GDGCHC	1 cut(s)	15
SfaNI	GCATC	3 cut(s)	388, 864, 1035
SfcI	CTRYAG	2 cut(s)	1113, 1183
SfiI	GGCCNNNNNGGCC	1 cut(s)	602
SfoI	GGCGCC	1 cut(s)	1358
Sfr274I	CTCGAG	1 cut(s)	227
Sfr303I	CCGCGG	1 cut(s)	397
SfuI	TTCGAA	2 cut(s)	1167, 1430
SgrBI	CCGCGG	1 cut(s)	397
SinI	GGWCC	3 cut(s)	475, 727, 808
SlaI	CTCGAG	1 cut(s)	227
SmaI	CCCGGG	1 cut(s)	293
SmiMI	CAYNNNNRTG	1 cut(s)	1127
SmlI	CTYRAG	3 cut(s)	140, 227, 428
SmoI	CTYRAG	3 cut(s)	140, 227, 428
Sse9I	AATT	6 cut(s)	107, 265, 307, 965, 1272, 1388
SspDI	GGCGCC	1 cut(s)	1356
SspI	AATATT	1 cut(s)	1200
SspMI	CTAG	1 cut(s)	1482
SstI	GAGCTC	1 cut(s)	15
StyI	CCWWGG	1 cut(s)	811
TaaI	ACNGT	2 cut(s)	770, 1342
TaiI	ACGT	4 cut(s)	420, 558, 834, 952
TaqI	TCGA	8 cut(s)	228, 269, 516, 885, 1024, 1055, 1167, 1430
TasI	AATT	6 cut(s)	107, 265, 307, 965, 1272, 1388
TatI	WGTACW	3 cut(s)	627, 681, 741
TauI	GCSGC	7 cut(s)	87, 90, 93, 215, 260, 399, 402
TfiI	GAWTC	8 cut(s)	491, 643, 1069, 1109, 1208, 1233, 1257, 1427
Tru1I	TTAA	3 cut(s)	306, 588, 1223
Tru9I	TTAA	3 cut(s)	306, 588, 1223
TscAI	CASTG	1 cut(s)	1270
TseFI	GTSAC	1 cut(s)	440
TseI	GCWGC	3 cut(s)	1031, 1159, 1217
Tsp45I	GTSAC	1 cut(s)	440
TspDTI	ATGAA	4 cut(s)	17, 901, 993, 1362
TspGWI	ACGGA	1 cut(s)	381
TspMI	CCCGGG	1 cut(s)	291
TspRI	CASTG	1 cut(s)	1270
Van91I	CCANNNNNTGG	1 cut(s)	451
VpaK11BI	GGWCC	3 cut(s)	475, 727, 808
XagI	CCTNNNNNAGG	1 cut(s)	470
XapI	RAATTY	1 cut(s)	1272
XceI	RCATGY	1 cut(s)	1126
XhoI	CTCGAG	1 cut(s)	227
XmaI	CCCGGG	1 cut(s)	291
XmiI	GTMKAC	1 cut(s)	1024
XmnI	GAANNNNTTC	2 cut(s)	236, 356
XspI	CTAG	1 cut(s)	1482
ZrmI	AGTACT	1 cut(s)	683
Zsp2I	ATGCAT	1 cut(s)	1050

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.