Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0011167

Physical Location & Seq

Genomic Coordinates Forward (+)

24990199 .. 24993899

Gene Structure 3701 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g22820.7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGCATGTTGATGAGAAAGTCACCTTGGTTTTGGGCTATGATCGAAAGAACCTTGTGGCAACCTTGGGCAAGGCCTTTGTGAAGGAAGTATCCTTGTGGCAAGCAAATATTTTACGTGACTGTCAAACTCTCGTCATTGATTTTACCAATTCACTTATGTTACAATGCATGTGTATTTATCAAACCTTGATCTTGGGCAAGGCATGTGTGAAGGGGATAGCCTTGGGCGCAATTGTCAAGCTCAACCTAATTAGAAAACCTTGGATCACAAGCCTTTGTTTAGTACATATCTTTTTAATTAACAACGCAGTAAGAAAACAATCACTTGGTCGTTTTATTTGTATTGTTAAGGAGCACTCCCTAAACTTGATCTTATACTATTACCATGTCACTGTTAATCAAGCTCCTTCATTGAACCTGACCAAATCTTTACCTTTAATATTAGGGAGTTTTAATGAAACACTATTGGTACTGTTTATTTTTAACAAAAAATCATATTTTTACATTTTCTGGTACTATTTACTTACACATTTATTTTTTTCCTTTAACATTAACTATCACCCCCTAAAACTTTACAAGTTATCAACCATCAACACAAAATCTTGCACTAGAAATGTTTTCTTTTGCGTTTTAATTTTTTTATTTGTAGGAAAGGGACAAAATGATTTAAGCACTAGTAATATGACAAGGGAAATTGTTTTTTTAAGGGATCAGTTGACTCATAGATGCATTTCAACATTCTTTTGGTATCATCGTGTGATTCACACAGCGTCAATACTCAAACCTAATACGTGTGTCAAATTGAATATTGAATATTCATGTCAAAATCAAAGTAATGTGTTTTTTATAAATCCTTTATCTTTCACCAATCATTCTCCATCAATCGTATGTATTACAAATAAAACATGCATTGATAATTCAATGATGAAAATGAATATTCCCACACCATTCACTATTTTAAGGATAAACCAACCTGTCTCCCCGTACACACCCTGGCGCACGTTAGCAGGGCCCCACCAGGCCCCCAAAACCACGGGTATGGTGAGTACTAGTAGTGAGCTCCAATCCAGCAAAGCCAACAAACCCTCCGCCCGTTCTTCCACCTCCCTCGATGCCGTCCACCGCAAAACCCTAACCCAGTTCTTCTCAGTGGCGCGAAAATCCTCGCTCCTCAAGCAATCCCTTGCCATCATCGTCGTCGTCCTCGTCCTATACGCCTTCCACAACACCTTCCACAACACCTTCCTCACGCCCGCCGCCACTTCCAAGCTCGAGAACGCCTTACCCTCCTTCTCCTCCGCCTCTTCCACTACTCAGGAAAATCAGCTTAATTTACAGGGAAAGCCTGTTAAAGTGTACCTCTACGATCTCCCTAAGCGGTTCACTTACGGAGTCATCGAGCATCATTCGCTTGCTCGCGGTGGCCGGCCCGACGACGACGTTTCGAAGCTCAAGTACCCGGGTCACCAGTACATGGGCGAGTGGTACCTGTTCCAGGACCTGCTCAAACCCGAATCGGAACGGGTCGGTTCGCCTGTTGAGAGGGTATTGGATCCGGAAGAGGCAGACTTGTTCTACGTGCCGTTCTTCTCGTCATTGAGCCTGATTGTTAACTCGGCGCAACCAGCTTCCGGGTCAGAGAAGCCGTTGTACAGCGACGAGGAGAATCAGGTGGACTTGATTGAGTGGCTAGAGTCGCAGGAGTACTGGAAGAGAAACAATGGCCGAGACCATGTGATCATGGCTTCGGACCCCAATGCTCTACACATAGTGATTGACAAAGTCAAAAATTGTGTACTGCTTGTTTCCGATTTTGGGCGGTTGAAGGAGGACCAAGGATCGCTTGTGAAGGACGTGATCGTGCCTTACTCTCACCGCATCAACACTTACACGGGCGACATCAGCGTCGAGAATAGGAACACCCTGTTGTTCTTCATGGGAAACCGCTTTCGAAAAGACGGTGGAAAGATACGTGATTTACTTTTCCAATTACTTGAAAATGAAGAGGATGTTATAGTAAAGCATGGAACCCAATCCACAAAGAATCGACGGGCTGCTACACGTGGGATGCATACGTCGAAGTTCTGTTTGAATCCGGCTGGTGATACTCCATCAGCCTGTCGACTTTTTGATTCTATAGTTAGTTTGTGTGTGCCTGTGATAATCAGTGATAGCATTGAGCTTCCATTCGAAGATGTTATAGACTACAGAAAGATAGCAATATTTGTAGAATCCAATGCAGCTTTAAAGCCAGGATTCTTAGTCTCAATGCTGAGAGGAATCCCCACTGAGAGAATTCTGGAGTACCAGACAGAGCTAAACAAGGTGAGGCATTATTTTCAATATGGAGAACCAAATGGATCGAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010393 GO:0010410 GO:0010413 GO:0010417 GO:0012505 GO:0015020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016763 GO:0017144 GO:0031221 GO:0031410 GO:0031982 GO:0031984 GO:0033692 GO:0034637 GO:0034645 GO:0035884 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045488 GO:0045489 GO:0045491 GO:0045492 GO:0047517 GO:0070589 GO:0070592 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0080116 GO:0097708 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

791

Amino Acids

89.96

Weight (kDa)

9.12

Isoelectric Point (pI)

41.26

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35100
fragaria_vesca	FvH4_3g12450 FvH4_3g12450
malus_domestica	MD05G1250200.v1.1 MD10G1231200.v1.1
prunus_persica	Prupe.4G113400_v2.0.a1
pyrus_communis	pycom03g23690 pycom05g22820 pycom10g19440
rosa_chinensis	RchiOBHm_Chr5g0020381
rosa_laevigata	RLG00000032518
rosa_multiflora	Rmu_sc0002087.1_g000001 Rmu_sc0007157.1_g000001
rosa_roxburghii	Rroxscaffold_1G00057880
rosa_rugosa	Rorug05G0058900
rosa_samantha	Rh5AG149100 Rh5BG148800 Rh5CG160400 Rh5DG147500
rosa_wichuraiana	Rw5G013220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	848
AasI	GACNNNNNNGTC	1 cut(s)	1904
Acc36I	ACCTGC	1 cut(s)	1509
Acc65I	GGTACC	1 cut(s)	1485
AccB1I	GGYRCC	1 cut(s)	1485
AccB7I	CCANNNNNTGG	1 cut(s)	1474
AccI	GTMKAC	1 cut(s)	2122
AccII	CGCG	2 cut(s)	1156, 1419
AccIII	TCCGGA	1 cut(s)	1555
AclWI	GGATC	6 cut(s)	272, 717, 1547, 1560, 1846, 2368
AcoI	YGGCCR	2 cut(s)	1423, 1723
AcsI	RAATTY	1 cut(s)	2295
AcvI	CACGTG	1 cut(s)	2063
AdeI	CACNNNGTG	1 cut(s)	1771
AfiI	CCNNNNNNNGG	4 cut(s)	1474, 1495, 1631, 1815
AflIII	ACRYGT	2 cut(s)	791, 2060
AgsI	TTSAA	9 cut(s)	415, 735, 805, 812, 921, 1825, 1997, 2092, 2342
AhlI	ACTAGT	2 cut(s)	674, 1049
AjiI	CACGTC	1 cut(s)	1855
AjnI	CCWGG	4 cut(s)	992, 1017, 1494, 2251
AjuI	GAANNNNNNNTTGG	2 cut(s)	8, 40
Alw21I	GWGCWC	2 cut(s)	357, 1062
Alw26I	GTCTC	3 cut(s)	982, 1722, 2269
AlwI	GGATC	6 cut(s)	272, 717, 1547, 1560, 1846, 2368
AlwNI	CAGNNNCTG	1 cut(s)	1501
Ama87I	CYCGRG	2 cut(s)	1271, 1459
Aor13HI	TCCGGA	1 cut(s)	1555
AoxI	GGCC	6 cut(s)	72, 1010, 1020, 1423, 1427, 1723
ApaI	GGGCCC	1 cut(s)	1014
ApeKI	GCWGC	2 cut(s)	2054, 2240
ApoI	RAATTY	1 cut(s)	2295
Asp718I	GGTACC	1 cut(s)	1485
AspLEI	GCGC	4 cut(s)	230, 999, 1156, 1621
AspS9I	GGNCC	7 cut(s)	1010, 1011, 1021, 1428, 1498, 1750, 1831
AsuC2I	CCSGG	3 cut(s)	1460, 1461, 1633
AsuHPI	GGTGA	8 cut(s)	13, 551, 856, 1054, 1457, 1865, 2114, 2338
AsuII	TTCGAA	3 cut(s)	1445, 1951, 2190
AvaI	CYCGRG	2 cut(s)	1271, 1459
AvaII	GGWCC	3 cut(s)	1498, 1750, 1831
BaeGI	GKGCMC	1 cut(s)	1014
BamHI	GGATCC	1 cut(s)	1552
BanI	GGYRCC	1 cut(s)	1485
BanII	GRGCYC	2 cut(s)	1014, 1062
BarI	GAAGNNNNNNTAC	4 cut(s)	1439, 1471, 1634, 1666
BbrPI	CACGTG	1 cut(s)	2063
Bbv12I	GWGCWC	2 cut(s)	357, 1062
BbvI	GCAGC	2 cut(s)	2041, 2252
BccI	CCATC	4 cut(s)	596, 886, 1196, 2119
BceAI	ACGGC	3 cut(s)	1100, 1567, 1630
BciT130I	CCWGG	4 cut(s)	994, 1019, 1496, 2253
BciVI	GTATCC	1 cut(s)	100
BclI	TGATCA	1 cut(s)	1737
BcnI	CCSGG	3 cut(s)	1460, 1461, 1633
BcoDI	GTCTC	3 cut(s)	982, 1722, 2269
BcuI	ACTAGT	2 cut(s)	674, 1049
BfaI	CTAG	4 cut(s)	609, 675, 1050, 1691
BfmI	CTRYAG	2 cut(s)	2136, 2206
BfuAI	ACCTGC	1 cut(s)	1509
BfuI	GTATCC	1 cut(s)	100
BisI	GCNGC	3 cut(s)	1257, 2055, 2241
BlsI	GCNGC	3 cut(s)	1258, 2056, 2242
BmcAI	AGTACT	2 cut(s)	1048, 1706
Bme1390I	CCNGG	7 cut(s)	994, 1019, 1460, 1461, 1496, 1633, 2253
Bme18I	GGWCC	3 cut(s)	1498, 1750, 1831
BmeT110I	CYCGRG	2 cut(s)	1271, 1459
BmgBI	CACGTC	1 cut(s)	1855
BmgT120I	GGNCC	7 cut(s)	1010, 1011, 1021, 1428, 1498, 1750, 1831
BmiI	GGNNCC	7 cut(s)	1012, 1013, 1023, 1487, 1554, 1752, 2029
BmrFI	CCNGG	7 cut(s)	994, 1019, 1460, 1461, 1496, 1633, 2253
BmrI	ACTGGG	1 cut(s)	1132
BmsI	GCATC	5 cut(s)	716, 1102, 1411, 1887, 2058
BmuI	ACTGGG	1 cut(s)	1132
BpmI	CTGGAG	1 cut(s)	2321
Bpu10I	CCTNAGC	1 cut(s)	1374
Bpu14I	TTCGAA	3 cut(s)	1445, 1951, 2190
BpuEI	CTTGAG	2 cut(s)	1157, 1436
BpuMI	CCSGG	3 cut(s)	1460, 1461, 1633
BsaAI	YACGTR	5 cut(s)	116, 792, 1579, 1973, 2063
BsaI	GGTCTC	1 cut(s)	1722
BsaJI	CCNNGG	8 cut(s)	24, 63, 222, 260, 992, 1032, 1459, 1834
BsaWI	WCCGGW	1 cut(s)	1555
BsaXI	ACNNNNNCTCC	2 cut(s)	1070, 1100
Bsc4I	CCNNNNNNNGG	4 cut(s)	1474, 1495, 1631, 1815
Bse118I	RCCGGY	1 cut(s)	1425
Bse1I	ACTGG	3 cut(s)	1138, 1468, 1712
BseAI	TCCGGA	1 cut(s)	1555
BseBI	CCWGG	4 cut(s)	994, 1019, 1496, 2253
BseDI	CCNNGG	8 cut(s)	24, 63, 222, 260, 992, 1032, 1459, 1834
BseGI	GGATG	2 cut(s)	2014, 2073
BseLI	CCNNNNNNNGG	4 cut(s)	1474, 1495, 1631, 1815
BseMII	CTCAG	4 cut(s)	1161, 1328, 2264, 2280
BseNI	ACTGG	3 cut(s)	1138, 1468, 1712
BseRI	GAGGAG	3 cut(s)	1160, 1285, 1676
BseSI	GKGCMC	1 cut(s)	1014
BseXI	GCAGC	2 cut(s)	2041, 2252
Bsh1236I	CGCG	2 cut(s)	1156, 1419
BshFI	GGCC	6 cut(s)	74, 1012, 1022, 1425, 1429, 1725
BshNI	GGYRCC	1 cut(s)	1485
BsiHKAI	GWGCWC	2 cut(s)	357, 1062
BsiHKCI	CYCGRG	2 cut(s)	1271, 1459
BsiSI	CCGG	5 cut(s)	1426, 1460, 1556, 1632, 2096
BslFI	GGGAC	1 cut(s)	669
BslI	CCNNNNNNNGG	4 cut(s)	1474, 1495, 1631, 1815
BsmAI	GTCTC	3 cut(s)	982, 1722, 2269
BsmFI	GGGAC	1 cut(s)	669
BsnI	GGCC	6 cut(s)	74, 1012, 1022, 1425, 1429, 1725
Bso31I	GGTCTC	1 cut(s)	1722
BsoBI	CYCGRG	2 cut(s)	1271, 1459
Bsp119I	TTCGAA	3 cut(s)	1445, 1951, 2190
Bsp120I	GGGCCC	1 cut(s)	1010
Bsp1286I	GDGCHC	3 cut(s)	357, 1014, 1062
Bsp13I	TCCGGA	1 cut(s)	1555
Bsp1407I	TGTACA	1 cut(s)	1650
BspANI	GGCC	6 cut(s)	74, 1012, 1022, 1425, 1429, 1725
BspCNI	CTCAG	4 cut(s)	1160, 1327, 2265, 2281
BspEI	TCCGGA	1 cut(s)	1555
BspFNI	CGCG	2 cut(s)	1156, 1419
BspLI	GGNNCC	7 cut(s)	1012, 1013, 1023, 1487, 1554, 1752, 2029
BspMI	ACCTGC	1 cut(s)	1509
BspPI	GGATC	6 cut(s)	272, 717, 1547, 1560, 1846, 2368
BspT104I	TTCGAA	3 cut(s)	1445, 1951, 2190
BspT107I	GGYRCC	1 cut(s)	1485
BspTNI	GGTCTC	1 cut(s)	1722
BsrFI	RCCGGY	1 cut(s)	1425
BsrGI	TGTACA	1 cut(s)	1650
BsrI	ACTGG	3 cut(s)	1138, 1468, 1712
BssAI	RCCGGY	1 cut(s)	1425
BssECI	CCNNGG	8 cut(s)	24, 63, 222, 260, 992, 1032, 1459, 1834
BssT1I	CCWWGG	5 cut(s)	24, 63, 222, 260, 1834
Bst2UI	CCWGG	4 cut(s)	994, 1019, 1496, 2253
Bst4CI	ACNGT	4 cut(s)	122, 394, 474, 1961
Bst6I	CTCTTC	4 cut(s)	1309, 1554, 1706, 1998
BstAUI	TGTACA	1 cut(s)	1650
BstBAI	YACGTR	5 cut(s)	116, 792, 1579, 1973, 2063
BstBI	TTCGAA	3 cut(s)	1445, 1951, 2190
BstC8I	GCNNGC	5 cut(s)	102, 1254, 1413, 1417, 1427
BstDEI	CTNAG	6 cut(s)	1147, 1314, 1374, 2260, 2273, 2289
BstDSI	CCRYGG	1 cut(s)	1032
BstEII	GGTNACC	1 cut(s)	1463
BstENI	CCTNNNNNAGG	1 cut(s)	1493
BstF5I	GGATG	2 cut(s)	2014, 2073
BstFNI	CGCG	2 cut(s)	1156, 1419
BstHHI	GCGC	4 cut(s)	230, 999, 1156, 1621
BstMAI	GTCTC	3 cut(s)	982, 1722, 2269
BstMWI	GCNNNNNNNGC	6 cut(s)	227, 1174, 1408, 1625, 1696, 1902
BstNI	CCWGG	4 cut(s)	994, 1019, 1496, 2253
BstNSI	RCATGY	4 cut(s)	8, 172, 207, 909
BstPI	GGTNACC	1 cut(s)	1463
BstSCI	CCNGG	7 cut(s)	992, 1017, 1458, 1459, 1494, 1631, 2251
BstSFI	CTRYAG	2 cut(s)	2136, 2206
BstSLI	GKGCMC	1 cut(s)	1014
BstUI	CGCG	2 cut(s)	1156, 1419
BstV1I	GCAGC	2 cut(s)	2041, 2252
BstX2I	RGATCY	1 cut(s)	1552
BstXI	CCANNNNNNTGG	1 cut(s)	1039
BstYI	RGATCY	1 cut(s)	1552
BsuI	GTATCC	1 cut(s)	100
BsuRI	GGCC	6 cut(s)	74, 1012, 1022, 1425, 1429, 1725
BtgI	CCRYGG	1 cut(s)	1032
BtrI	CACGTC	1 cut(s)	1855
BtsCI	GGATG	2 cut(s)	2014, 2073
BtsIMutI	CAGTG	4 cut(s)	390, 1155, 2173, 2286
BveI	ACCTGC	1 cut(s)	1509
Cac8I	GCNNGC	5 cut(s)	102, 1254, 1413, 1417, 1427
CaiI	CAGNNNCTG	1 cut(s)	1501
CfoI	GCGC	4 cut(s)	230, 999, 1156, 1621
Cfr10I	RCCGGY	1 cut(s)	1425
Cfr13I	GGNCC	7 cut(s)	1010, 1011, 1021, 1428, 1498, 1750, 1831
Cfr9I	CCCGGG	1 cut(s)	1459
CseI	GACGC	2 cut(s)	759, 1894
DdeI	CTNAG	6 cut(s)	1147, 1314, 1374, 2260, 2273, 2289
DraI	TTTAAA	1 cut(s)	2247
DraIII	CACNNNGTG	1 cut(s)	1771
DrdI	GACNNNNNNGTC	1 cut(s)	1904
DseDI	GACNNNNNNGTC	1 cut(s)	1904
EaeI	YGGCCR	2 cut(s)	1423, 1723
Eam1104I	CTCTTC	4 cut(s)	1309, 1554, 1706, 1998
EarI	CTCTTC	4 cut(s)	1309, 1554, 1706, 1998
EciI	GGCGGA	2 cut(s)	1078, 1288
Ecl136II	GAGCTC	1 cut(s)	1060
Eco130I	CCWWGG	5 cut(s)	24, 63, 222, 260, 1834
Eco147I	AGGCCT	1 cut(s)	74
Eco24I	GRGCYC	2 cut(s)	1014, 1062
Eco31I	GGTCTC	1 cut(s)	1722
Eco47I	GGWCC	3 cut(s)	1498, 1750, 1831
Eco53kI	GAGCTC	1 cut(s)	1060
Eco72I	CACGTG	1 cut(s)	2063
Eco88I	CYCGRG	2 cut(s)	1271, 1459
Eco91I	GGTNACC	1 cut(s)	1463
EcoICRI	GAGCTC	1 cut(s)	1060
EcoNI	CCTNNNNNAGG	1 cut(s)	1493
EcoO109I	RGGNCCY	4 cut(s)	1010, 1011, 1021, 1498
EcoO65I	GGTNACC	1 cut(s)	1463
EcoRI	GAATTC	1 cut(s)	2295
EcoRII	CCWGG	4 cut(s)	992, 1017, 1494, 2251
EcoT14I	CCWWGG	5 cut(s)	24, 63, 222, 260, 1834
EcoT22I	ATGCAT	5 cut(s)	6, 170, 731, 911, 2073
EcoT38I	GRGCYC	2 cut(s)	1014, 1062
ErhI	CCWWGG	5 cut(s)	24, 63, 222, 260, 1834
FalI	AAGNNNNNCTT	2 cut(s)	1827, 1859
FaqI	GGGAC	1 cut(s)	669
FauI	CCCGC	1 cut(s)	1261
FbaI	TGATCA	1 cut(s)	1737
FblI	GTMKAC	1 cut(s)	2122
Fnu4HI	GCNGC	3 cut(s)	1257, 2055, 2241
FokI	GGATG	2 cut(s)	2021, 2080
FriOI	GRGCYC	2 cut(s)	1014, 1062
FseI	GGCCGGCC	1 cut(s)	1429
Fsp4HI	GCNGC	3 cut(s)	1257, 2055, 2241
FspBI	CTAG	4 cut(s)	609, 675, 1050, 1691
GlaI	GCGC	4 cut(s)	229, 998, 1155, 1620
GluI	GCNGC	3 cut(s)	1257, 2055, 2241
GsuI	CTGGAG	1 cut(s)	2321
HaeIII	GGCC	6 cut(s)	74, 1012, 1022, 1425, 1429, 1725
HapII	CCGG	5 cut(s)	1426, 1460, 1556, 1632, 2096
HgaI	GACGC	2 cut(s)	759, 1894
HhaI	GCGC	4 cut(s)	230, 999, 1156, 1621
Hin6I	GCGC	4 cut(s)	228, 997, 1154, 1619
HinP1I	GCGC	4 cut(s)	228, 997, 1154, 1619
HincII	GTYRAC	3 cut(s)	717, 1612, 2123
HindII	GTYRAC	3 cut(s)	717, 1612, 2123
HpaI	GTTAAC	1 cut(s)	1612
HpaII	CCGG	5 cut(s)	1426, 1460, 1556, 1632, 2096
HphI	GGTGA	8 cut(s)	13, 551, 856, 1054, 1457, 1865, 2114, 2338
Hpy166II	GTNNAC	9 cut(s)	717, 987, 1120, 1357, 1383, 1612, 1675, 1796, 2123
Hpy188I	TCNGA	4 cut(s)	1519, 1639, 1750, 1810
Hpy188III	TCNNGA	6 cut(s)	1273, 1316, 1556, 1909, 2300, 2364
Hpy8I	GTNNAC	9 cut(s)	717, 987, 1120, 1357, 1383, 1612, 1675, 1796, 2123
Hpy99I	CGWCG	9 cut(s)	1199, 1202, 1436, 1439, 1442, 1661, 1910, 2052, 2080
HpyAV	CCTTC	9 cut(s)	76, 205, 417, 1228, 1240, 1252, 1300, 1819, 1843
HpyCH4III	ACNGT	4 cut(s)	122, 394, 474, 1961
HpyCH4IV	ACGT	9 cut(s)	115, 791, 1001, 1440, 1578, 1854, 1972, 2062, 2075
HpyCH4V	TGCA	7 cut(s)	4, 168, 606, 729, 909, 2071, 2240
HpyF10VI	GCNNNNNNNGC	6 cut(s)	227, 1174, 1408, 1625, 1696, 1902
HpyF3I	CTNAG	6 cut(s)	1147, 1314, 1374, 2260, 2273, 2289
HpySE526I	ACGT	9 cut(s)	115, 791, 1001, 1440, 1578, 1854, 1972, 2062, 2075
HspAI	GCGC	4 cut(s)	228, 997, 1154, 1619
Kpn2I	TCCGGA	1 cut(s)	1555
KpnI	GGTACC	1 cut(s)	1489
KroI	GCCGGC	1 cut(s)	1425
KroNI	GCCGGC	1 cut(s)	1427
Ksp22I	TGATCA	1 cut(s)	1737
KspAI	GTTAAC	1 cut(s)	1612
LmnI	GCTCC	4 cut(s)	352, 409, 1065, 1173
Lsp1109I	GCAGC	2 cut(s)	2041, 2252
LweI	GCATC	5 cut(s)	716, 1102, 1411, 1887, 2058
MaeI	CTAG	4 cut(s)	609, 675, 1050, 1691
MaeII	ACGT	9 cut(s)	115, 791, 1001, 1440, 1578, 1854, 1972, 2062, 2075
MaeIII	GTNAC	5 cut(s)	19, 116, 159, 388, 1463
MboII	GAAGA	9 cut(s)	1089, 1135, 1296, 1571, 1579, 1723, 1924, 2015, 2204
MfeI	CAATTG	1 cut(s)	231
MflI	RGATCY	1 cut(s)	1552
MhlI	GDGCHC	3 cut(s)	357, 1014, 1062
MlyI	GAGTC	3 cut(s)	712, 1401, 1703
Mph1103I	ATGCAT	5 cut(s)	6, 170, 731, 911, 2073
MroI	TCCGGA	1 cut(s)	1555
MroNI	GCCGGC	1 cut(s)	1425
MslI	CAYNNNNRTG	2 cut(s)	9, 1037
MspI	CCGG	5 cut(s)	1426, 1460, 1556, 1632, 2096
MspR9I	CCNGG	7 cut(s)	994, 1019, 1460, 1461, 1496, 1633, 2253
MunI	CAATTG	1 cut(s)	231
MvaI	CCWGG	4 cut(s)	994, 1019, 1496, 2253
MvnI	CGCG	2 cut(s)	1156, 1419
MwoI	GCNNNNNNNGC	6 cut(s)	227, 1174, 1408, 1625, 1696, 1902
NaeI	GCCGGC	1 cut(s)	1427
NciI	CCSGG	3 cut(s)	1460, 1461, 1633
NgoMIV	GCCGGC	1 cut(s)	1425
NlaIV	GGNNCC	7 cut(s)	1012, 1013, 1023, 1487, 1554, 1752, 2029
NmeAIII	GCCGAG	2 cut(s)	1595, 1751
NmuCI	GTSAC	4 cut(s)	19, 116, 388, 1463
NsiI	ATGCAT	5 cut(s)	6, 170, 731, 911, 2073
NspI	RCATGY	4 cut(s)	8, 172, 207, 909
NspV	TTCGAA	3 cut(s)	1445, 1951, 2190
PacI	TTAATTAA	1 cut(s)	300
PaeR7I	CTCGAG	1 cut(s)	1271
PceI	AGGCCT	1 cut(s)	74
PcsI	WCGNNNNNNNCGW	2 cut(s)	1203, 1395
PdiI	GCCGGC	1 cut(s)	1427
PfeI	GAWTC	9 cut(s)	760, 1514, 1666, 2044, 2092, 2132, 2231, 2256, 2280
PflFI	GACNNNGTC	1 cut(s)	1781
PflMI	CCANNNNNTGG	1 cut(s)	1474
PfoI	TCCNGGA	1 cut(s)	1494
PkrI	GCNGC	3 cut(s)	1258, 2056, 2242
PleI	GAGTC	3 cut(s)	712, 1400, 1702
PmaCI	CACGTG	1 cut(s)	2063
PmlI	CACGTG	1 cut(s)	2063
PpsI	GAGTC	3 cut(s)	712, 1400, 1702
Ppu21I	YACGTR	5 cut(s)	116, 792, 1579, 1973, 2063
PpuMI	RGGWCCY	1 cut(s)	1498
PsiI	TTATAA	1 cut(s)	848
Psp124BI	GAGCTC	1 cut(s)	1062
Psp5II	RGGWCCY	1 cut(s)	1498
Psp6I	CCWGG	4 cut(s)	992, 1017, 1494, 2251
PspCI	CACGTG	1 cut(s)	2063
PspEI	GGTNACC	1 cut(s)	1463
PspGI	CCWGG	4 cut(s)	992, 1017, 1494, 2251
PspN4I	GGNNCC	7 cut(s)	1012, 1013, 1023, 1487, 1554, 1752, 2029
PspOMI	GGGCCC	1 cut(s)	1010
PspPI	GGNCC	7 cut(s)	1010, 1011, 1021, 1428, 1498, 1750, 1831
PspPPI	RGGWCCY	1 cut(s)	1498
PstNI	CAGNNNCTG	1 cut(s)	1501
PsuI	RGATCY	1 cut(s)	1552
PsyI	GACNNNGTC	1 cut(s)	1781
RigI	GGCCGGCC	1 cut(s)	1429
RseI	CAYNNNNRTG	2 cut(s)	9, 1037
SacI	GAGCTC	1 cut(s)	1062
SalI	GTCGAC	1 cut(s)	2121
SatI	GCNGC	3 cut(s)	1257, 2055, 2241
Sau96I	GGNCC	7 cut(s)	1010, 1011, 1021, 1428, 1498, 1750, 1831
ScaI	AGTACT	2 cut(s)	1048, 1706
SchI	GAGTC	3 cut(s)	712, 1401, 1703
ScrFI	CCNGG	7 cut(s)	994, 1019, 1460, 1461, 1496, 1633, 2253
SduI	GDGCHC	3 cut(s)	357, 1014, 1062
SfaNI	GCATC	5 cut(s)	716, 1102, 1411, 1887, 2058
SfcI	CTRYAG	2 cut(s)	2136, 2206
Sfr274I	CTCGAG	1 cut(s)	1271
SfuI	TTCGAA	3 cut(s)	1445, 1951, 2190
SinI	GGWCC	3 cut(s)	1498, 1750, 1831
SlaI	CTCGAG	1 cut(s)	1271
SmaI	CCCGGG	1 cut(s)	1461
SmiMI	CAYNNNNRTG	2 cut(s)	9, 1037
SmlI	CTYRAG	3 cut(s)	1172, 1271, 1451
SmoI	CTYRAG	3 cut(s)	1172, 1271, 1451
SpeI	ACTAGT	2 cut(s)	674, 1049
SseBI	AGGCCT	1 cut(s)	74
SspI	AATATT	6 cut(s)	109, 441, 808, 815, 937, 2223
SspMI	CTAG	4 cut(s)	609, 675, 1050, 1691
SstI	GAGCTC	1 cut(s)	1062
StuI	AGGCCT	1 cut(s)	74
StyD4I	CCNGG	7 cut(s)	992, 1017, 1458, 1459, 1494, 1631, 2251
StyI	CCWWGG	5 cut(s)	24, 63, 222, 260, 1834
TaaI	ACNGT	4 cut(s)	122, 394, 474, 1961
TaiI	ACGT	9 cut(s)	118, 794, 1004, 1443, 1581, 1857, 1975, 2065, 2078
TatI	WGTACW	6 cut(s)	283, 1046, 1470, 1650, 1704, 1795
TauI	GCSGC	1 cut(s)	1259
TfiI	GAWTC	9 cut(s)	760, 1514, 1666, 2044, 2092, 2132, 2231, 2256, 2280
TscAI	CASTG	4 cut(s)	397, 1155, 2173, 2293
TseFI	GTSAC	4 cut(s)	19, 116, 388, 1463
TseI	GCWGC	2 cut(s)	2054, 2240
Tsp45I	GTSAC	4 cut(s)	19, 116, 388, 1463
TspDTI	ATGAA	7 cut(s)	399, 471, 807, 941, 947, 1924, 2016
TspGWI	ACGGA	1 cut(s)	1404
TspMI	CCCGGG	1 cut(s)	1459
TspRI	CASTG	4 cut(s)	397, 1155, 2173, 2293
Tth111I	GACNNNGTC	1 cut(s)	1781
Van91I	CCANNNNNTGG	1 cut(s)	1474
VpaK11BI	GGWCC	3 cut(s)	1498, 1750, 1831
XagI	CCTNNNNNAGG	1 cut(s)	1493
XapI	RAATTY	1 cut(s)	2295
XceI	RCATGY	4 cut(s)	8, 172, 207, 909
XhoI	CTCGAG	1 cut(s)	1271
XmaI	CCCGGG	1 cut(s)	1459
XmiI	GTMKAC	1 cut(s)	2122
XspI	CTAG	4 cut(s)	609, 675, 1050, 1691
ZrmI	AGTACT	2 cut(s)	1048, 1706
Zsp2I	ATGCAT	5 cut(s)	6, 170, 731, 911, 2073

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.