Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012786

Physical Location & Seq

Genomic Coordinates Reverse (-)

33339497 .. 33343622

Gene Structure 4126 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1237200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 603 bp

ATGGCCACCGCCGGGAACAAGAACATCAATGCCAAATTGGTGCTTCTTGGGGATGTTGGAGCTGGGAAGTCTAGTCTGGTGTTGCGCTTTGTAAAAGGACAATTCATTGAATTTCAGGAATCAACAATAGGTGCTGCCTTCTTCTCACAAACATTGGCTGTAAACGATGCAACTGTAAAATTTGAGATTTGGGATACAGCAGGTCAAGAGAGGTACCATAGTTTGGCGCCAATGTATTACAGAGGAGCTGCTGCTGCAATTATTGTGTATGATTTAACAAATCAAGCCTCATTTGAGCGAGCAAAAAAATGGGTCCTCGAACTCAAGTCACAAGGTAACCCAAACATGGTTATGGCACTAGCTGGTAATAAAGCGGATCTGGTGGAGGCCAGGAAAGTGGCCGCAGAGGATGCACAATCATATGCTCAAGAGAATGGTCTTTTCTTCCTGGAAACCTCTGCAAAAACTGCAGACAATGTCAATGACATTTTCTATGAGATAGCAAAGAGATTACCTCGAGTGCAGCCTGTGCAGAACCCCGCAGGAATGGTTCTTGTGGACAGACCTTCTGAAAGGGTGGCAAGCTCGTCTTGTTGCTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005783 GO:0005886 GO:0006810 GO:0006886 GO:0006996 GO:0007033 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0017076 GO:0019001 GO:0030139 GO:0031410 GO:0031982 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0033036 GO:0034613 GO:0035639 GO:0036094 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0045324 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0097708 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04014 ko04144 ko04145 ko04962 ko05014 ko05146 ko05152 map04014 map04144 map04145 map04962 map05014 map05146 map05152

Pfam Domains

Protein Families

Ras

Protein Analysis

201

Amino Acids

21.78

Weight (kDa)

6.6

Isoelectric Point (pI)

35.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	6 - 135	3.2e-12	ADP-ribosylation factor family
Ras	PF00071	12 - 171	1.9e-58	Ras family
Roc	PF08477	12 - 126	1.2e-33	Ras of Complex, Roc, domain of DAPkinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19640 AT5G45130 AT5G45130
fragaria_vesca	FvH4_3g11910
malus_domestica	MD05G1256700.v1.1 MD10G1237200.v1.1
prunus_persica	Prupe.4G107100_v2.0.a1 Prupe.4G107100_v2.0.a1
pyrus_communis	pycom05g23600 pycom10g19850
rosa_chinensis	RchiOBHm_Chr5g0019441
rosa_laevigata	RLG00000032443
rosa_multiflora	Rmu_sc0002759.1_g000043
rosa_roxburghii	Rroxscaffold_1G00058620 Rroxscaffold_1G00074300
rosa_rugosa	Rorug05G0051000
rosa_samantha	Rh5CG151800
rosa_wichuraiana	Rw5G012560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	191
Acc65I	GGTACC	1 cut(s)	213
AccB1I	GGYRCC	2 cut(s)	213, 226
AccB7I	CCANNNNNTGG	1 cut(s)	223
AciI	CCGC	4 cut(s)	9, 374, 402, 540
AclWI	GGATC	1 cut(s)	384
AcoI	YGGCCR	2 cut(s)	3, 399
AcsI	RAATTY	2 cut(s)	110, 179
AcyI	GRCGYC	1 cut(s)	227
AfaI	GTAC	1 cut(s)	215
AfiI	CCNNNNNNNGG	3 cut(s)	12, 223, 346
AgsI	TTSAA	1 cut(s)	110
AjnI	CCWGG	2 cut(s)	389, 447
AluBI	AGCT	4 cut(s)	62, 248, 362, 585
AluI	AGCT	4 cut(s)	62, 248, 362, 585
AlwI	GGATC	1 cut(s)	384
Ama87I	CYCGRG	1 cut(s)	516
AoxI	GGCC	3 cut(s)	3, 387, 399
ApeKI	GCWGC	5 cut(s)	134, 248, 251, 254, 523
ApoI	RAATTY	2 cut(s)	110, 179
Asp718I	GGTACC	1 cut(s)	213
AspLEI	GCGC	2 cut(s)	87, 229
AspS9I	GGNCC	1 cut(s)	313
AsuC2I	CCSGG	1 cut(s)	13
AvaI	CYCGRG	1 cut(s)	516
AvaII	GGWCC	1 cut(s)	313
BaeI	ACNNNNGTAYC	2 cut(s)	205, 238
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	2 cut(s)	213, 226
BbvI	GCAGC	5 cut(s)	121, 235, 238, 241, 535
BciT130I	CCWGG	2 cut(s)	391, 449
BciVI	GTATCC	1 cut(s)	187
BcnI	CCSGG	1 cut(s)	13
BfaI	CTAG	2 cut(s)	72, 359
BfmI	CTRYAG	1 cut(s)	468
BfoI	RGCGCY	1 cut(s)	230
BfuAI	ACCTGC	1 cut(s)	191
BfuI	GTATCC	1 cut(s)	187
BisI	GCNGC	6 cut(s)	135, 249, 252, 255, 402, 524
BlsI	GCNGC	6 cut(s)	136, 250, 253, 256, 403, 525
Bme1390I	CCNGG	3 cut(s)	13, 391, 449
Bme18I	GGWCC	1 cut(s)	313
BmeT110I	CYCGRG	1 cut(s)	516
BmgT120I	GGNCC	1 cut(s)	313
BmiI	GGNNCC	3 cut(s)	215, 228, 314
BmrFI	CCNGG	3 cut(s)	13, 391, 449
BmsI	GCATC	2 cut(s)	157, 400
BplI	GAGNNNNNCTC	2 cut(s)	499, 531
BpuEI	CTTGAG	2 cut(s)	308, 411
BpuMI	CCSGG	1 cut(s)	13
BsaHI	GRCGYC	1 cut(s)	227
Bsc4I	CCNNNNNNNGG	3 cut(s)	12, 223, 346
BseBI	CCWGG	2 cut(s)	391, 449
BseGI	GGATG	2 cut(s)	58, 415
BseLI	CCNNNNNNNGG	3 cut(s)	12, 223, 346
BseRI	GAGGAG	1 cut(s)	258
BseXI	GCAGC	5 cut(s)	121, 235, 238, 241, 535
BseYI	CCCAGC	1 cut(s)	62
BsgI	GTGCAG	2 cut(s)	542, 551
BshFI	GGCC	3 cut(s)	5, 389, 401
BshNI	GGYRCC	2 cut(s)	213, 226
BsiHKCI	CYCGRG	1 cut(s)	516
BsiSI	CCGG	1 cut(s)	12
BslI	CCNNNNNNNGG	3 cut(s)	12, 223, 346
BsnI	GGCC	3 cut(s)	5, 389, 401
BsoBI	CYCGRG	1 cut(s)	516
Bsp143I	GATC	1 cut(s)	376
BspACI	CCGC	4 cut(s)	9, 374, 402, 540
BspANI	GGCC	3 cut(s)	5, 389, 401
BspLI	GGNNCC	3 cut(s)	215, 228, 314
BspMAI	CTGCAG	1 cut(s)	472
BspMI	ACCTGC	1 cut(s)	191
BspPI	GGATC	1 cut(s)	384
BspT107I	GGYRCC	2 cut(s)	213, 226
BssMI	GATC	1 cut(s)	376
BssNI	GRCGYC	1 cut(s)	227
Bst2UI	CCWGG	2 cut(s)	391, 449
Bst4CI	ACNGT	1 cut(s)	175
BstACI	GRCGYC	1 cut(s)	227
BstAPI	GCANNNNNTGC	3 cut(s)	410, 467, 529
BstC8I	GCNNGC	2 cut(s)	300, 583
BstEII	GGTNACC	1 cut(s)	335
BstF5I	GGATG	2 cut(s)	58, 415
BstH2I	RGCGCY	1 cut(s)	230
BstHHI	GCGC	2 cut(s)	87, 229
BstKTI	GATC	1 cut(s)	379
BstMBI	GATC	1 cut(s)	376
BstMWI	GCNNNNNNNGC	4 cut(s)	254, 410, 467, 529
BstNI	CCWGG	2 cut(s)	391, 449
BstPI	GGTNACC	1 cut(s)	335
BstSCI	CCNGG	3 cut(s)	11, 389, 447
BstSFI	CTRYAG	1 cut(s)	468
BstV1I	GCAGC	5 cut(s)	121, 235, 238, 241, 535
BstX2I	RGATCY	1 cut(s)	376
BstXI	CCANNNNNNTGG	1 cut(s)	397
BstYI	RGATCY	1 cut(s)	376
BsuI	GTATCC	1 cut(s)	187
BsuRI	GGCC	3 cut(s)	5, 389, 401
BtsCI	GGATG	2 cut(s)	58, 415
BveI	ACCTGC	1 cut(s)	191
Cac8I	GCNNGC	2 cut(s)	300, 583
CfoI	GCGC	2 cut(s)	87, 229
Cfr13I	GGNCC	1 cut(s)	313
Csp6I	GTAC	1 cut(s)	214
CviAII	CATG	1 cut(s)	346
CviQI	GTAC	1 cut(s)	214
DinI	GGCGCC	1 cut(s)	228
DpnI	GATC	1 cut(s)	378
DpnII	GATC	1 cut(s)	376
EaeI	YGGCCR	2 cut(s)	3, 399
Eco47I	GGWCC	1 cut(s)	313
Eco88I	CYCGRG	1 cut(s)	516
Eco91I	GGTNACC	1 cut(s)	335
EcoO109I	RGGNCCY	1 cut(s)	313
EcoO65I	GGTNACC	1 cut(s)	335
EcoRII	CCWGG	2 cut(s)	389, 447
EgeI	GGCGCC	1 cut(s)	228
EheI	GGCGCC	1 cut(s)	228
FaeI	CATG	1 cut(s)	349
FaiI	YATR	8 cut(s)	219, 270, 347, 353, 421, 423, 495, 601
FalI	AAGNNNNNCTT	1 cut(s)	574
FatI	CATG	1 cut(s)	345
FauI	CCCGC	1 cut(s)	547
FauNDI	CATATG	1 cut(s)	421
Fnu4HI	GCNGC	6 cut(s)	135, 249, 252, 255, 402, 524
FokI	GGATG	2 cut(s)	65, 422
Fsp4HI	GCNGC	6 cut(s)	135, 249, 252, 255, 402, 524
FspBI	CTAG	2 cut(s)	72, 359
GlaI	GCGC	2 cut(s)	86, 228
GluI	GCNGC	6 cut(s)	135, 249, 252, 255, 402, 524
GsaI	CCCAGC	1 cut(s)	66
HaeII	RGCGCY	1 cut(s)	230
HaeIII	GGCC	3 cut(s)	5, 389, 401
HapII	CCGG	1 cut(s)	12
HhaI	GCGC	2 cut(s)	87, 229
Hin1I	GRCGYC	1 cut(s)	227
Hin1II	CATG	1 cut(s)	349
Hin6I	GCGC	2 cut(s)	85, 227
HinP1I	GCGC	2 cut(s)	85, 227
HinfI	GANTC	1 cut(s)	119
HpaII	CCGG	1 cut(s)	12
Hpy166II	GTNNAC	2 cut(s)	163, 559
Hpy188I	TCNGA	1 cut(s)	571
Hpy188III	TCNNGA	3 cut(s)	116, 206, 428
Hpy8I	GTNNAC	2 cut(s)	163, 559
HpyAV	CCTTC	2 cut(s)	148, 576
HpyCH4III	ACNGT	1 cut(s)	175
HpyCH4V	TGCA	7 cut(s)	170, 257, 413, 461, 470, 523, 532
HpyF10VI	GCNNNNNNNGC	4 cut(s)	254, 410, 467, 529
Hsp92I	GRCGYC	1 cut(s)	227
Hsp92II	CATG	1 cut(s)	349
HspAI	GCGC	2 cut(s)	85, 227
KasI	GGCGCC	1 cut(s)	226
KpnI	GGTACC	1 cut(s)	217
Kzo9I	GATC	1 cut(s)	376
LmnI	GCTCC	2 cut(s)	59, 245
Lsp1109I	GCAGC	5 cut(s)	121, 235, 238, 241, 535
LweI	GCATC	2 cut(s)	157, 400
MaeI	CTAG	2 cut(s)	72, 359
MaeIII	GTNAC	2 cut(s)	327, 335
MalI	GATC	1 cut(s)	378
MboI	GATC	1 cut(s)	376
MboII	GAAGA	2 cut(s)	133, 436
MflI	RGATCY	1 cut(s)	376
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	35, 101, 110, 179, 258
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	227
MmeI	TCCRAC	1 cut(s)	37
MnlI	CCTC	8 cut(s)	204, 236, 298, 326, 379, 400, 466, 525
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	275
MslI	CAYNNNNRTG	1 cut(s)	350
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	1 cut(s)	12
MspR9I	CCNGG	3 cut(s)	13, 391, 449
MvaI	CCWGG	2 cut(s)	391, 449
MwoI	GCNNNNNNNGC	4 cut(s)	254, 410, 467, 529
NarI	GGCGCC	1 cut(s)	227
NciI	CCSGG	1 cut(s)	13
NdeI	CATATG	1 cut(s)	421
NdeII	GATC	1 cut(s)	376
NlaIII	CATG	1 cut(s)	349
NlaIV	GGNNCC	3 cut(s)	215, 228, 314
NmuCI	GTSAC	1 cut(s)	327
PaeR7I	CTCGAG	1 cut(s)	516
PfeI	GAWTC	1 cut(s)	119
PflFI	GACNNNGTC	1 cut(s)	476
PflMI	CCANNNNNTGG	1 cut(s)	223
PfoI	TCCNGGA	1 cut(s)	447
PkrI	GCNGC	6 cut(s)	136, 250, 253, 256, 403, 525
PluTI	GGCGCC	1 cut(s)	230
PpuMI	RGGWCCY	1 cut(s)	313
Psp5II	RGGWCCY	1 cut(s)	313
Psp6I	CCWGG	2 cut(s)	389, 447
PspEI	GGTNACC	1 cut(s)	335
PspFI	CCCAGC	1 cut(s)	62
PspGI	CCWGG	2 cut(s)	389, 447
PspN4I	GGNNCC	3 cut(s)	215, 228, 314
PspPI	GGNCC	1 cut(s)	313
PspPPI	RGGWCCY	1 cut(s)	313
PspXI	VCTCGAGB	1 cut(s)	516
PstI	CTGCAG	1 cut(s)	472
PsuI	RGATCY	1 cut(s)	376
PsyI	GACNNNGTC	1 cut(s)	476
RsaI	GTAC	1 cut(s)	215
RsaNI	GTAC	1 cut(s)	214
RseI	CAYNNNNRTG	1 cut(s)	350
SaqAI	TTAA	1 cut(s)	275
SatI	GCNGC	6 cut(s)	135, 249, 252, 255, 402, 524
Sau3AI	GATC	1 cut(s)	376
Sau96I	GGNCC	1 cut(s)	313
ScrFI	CCNGG	3 cut(s)	13, 391, 449
SfaNI	GCATC	2 cut(s)	157, 400
SfcI	CTRYAG	1 cut(s)	468
SfoI	GGCGCC	1 cut(s)	228
Sfr274I	CTCGAG	1 cut(s)	516
SinI	GGWCC	1 cut(s)	313
SlaI	CTCGAG	1 cut(s)	516
SmiMI	CAYNNNNRTG	1 cut(s)	350
SmlI	CTYRAG	3 cut(s)	323, 426, 516
SmoI	CTYRAG	3 cut(s)	323, 426, 516
Sse9I	AATT	5 cut(s)	35, 101, 110, 179, 258
SsiI	CCGC	4 cut(s)	9, 374, 402, 540
SspDI	GGCGCC	1 cut(s)	226
SspMI	CTAG	2 cut(s)	72, 359
StyD4I	CCNGG	3 cut(s)	11, 389, 447
TaaI	ACNGT	1 cut(s)	175
TaqI	TCGA	2 cut(s)	318, 517
TasI	AATT	5 cut(s)	35, 101, 110, 179, 258
TauI	GCSGC	1 cut(s)	404
TfiI	GAWTC	1 cut(s)	119
Tru1I	TTAA	1 cut(s)	275
Tru9I	TTAA	1 cut(s)	275
TseFI	GTSAC	1 cut(s)	327
TseI	GCWGC	5 cut(s)	134, 248, 251, 254, 523
Tsp45I	GTSAC	1 cut(s)	327
TspDTI	ATGAA	1 cut(s)	94
Tth111I	GACNNNGTC	1 cut(s)	476
Van91I	CCANNNNNTGG	1 cut(s)	223
VpaK11BI	GGWCC	1 cut(s)	313
XapI	RAATTY	2 cut(s)	110, 179
XhoI	CTCGAG	1 cut(s)	516
XspI	CTAG	2 cut(s)	72, 359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.