Rorug05G0051000

Haloacid dehalogenase-like hydrolase domain-containing protein At3g48420

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012786
Physical Location & Seq
Forward (+)
4444007 .. 4447195
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0051000.1

Sequence Viewer

Length: 1503 bp
ATGAACCAGTCATGCATACCGGCCGTTTGTTTCTTTTTTCTGACTGGCCAGGATATATTTCTGTTAAAACAAATCCGGCCAGCCTTCTTCTTCATTCTGGCAACTATTGTGGAGCCTCAGCCTGTTTTGGGATCTAGAAGAACATTGCAACAACAGACTGGGATCAGTACTAGTAGTGATGAGGATCTTGTGACCAACTTGCCTGGCCAGCCTGCCGTAGACTTCAAGCACTATGCCGGCTATGTCACTGTGAACGAAACAAACGGACGGGCACTCTTTTACTGGTTCTTTGAGGCTGCTACCACTCCAGATCAAAAACCTCTTGTTTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCCAGCCAAGAGATTGGTCCATTCCTATTGGACATTAAGAGCCAGGGACTCATATTTAATAACTACTCATGGAATAAAGAGGCCAACGTCCTATTCTTGGAATCTCCGATTGGGGTTGGCTTTTCATACACCAACACAACTTCTGATTTTGATAAGCTAGGAGATAACTTCACAGCGCAAGATTCATACACATTCCTGCATAAGTGGTTTCTCAAATTCCCATCATACAGAAACCGGACCTTGTACATTGCAGGCGAGAGTTATGCAGGAAAATATATTCCGGAGCTAGCTGAGTTCATCTTTGATAGGAACTCAGATCCTTCCCTCCATATTGATCTCAAGGGTATCCTGATGGGTAATCCTGAAACAGATGATGCTGAGGATTGGAGAGGTCTGGTGGATTATGCTTGGAGTCATGCTGTGGTTTCGGACGAAACTCACAAATTAATAAGCGAGTACTGTGATTTCCACAGTAATAATACTTGGGAAAATAAAAATTGTACACAAGGTGTGGATGAAGTATTTAAACAGTACAAGGAGATAGATATATACAGCCTTTACACCCTGACCTGCACTACAAATGATACAGCAACTTCGAATACTTCTGATTCAAAAGTTCAAGTCATGACGATGAAGCACACATCTACAATGATACCAAGGATTATGGGAGGCTATGATGCATGTCTGGATGGTTATTCGAAAAAATTCTTCAACAGAGCAGATGTGCAAAAGGCCCTCCATGCCAGTGATGGTCTCCAGCTCAAGAACTGGAGCATCTGCAATAATCAAATATTTACCAATTGGTCAGATTCAAAGCCATCTCTTCTTCCCATATACAAGAAACTCATTGCAGCAGGACTAAGAATATGGTTGTACAGTGGAGACACGGATGGAAGAGTACCTGTGCTGTCCACAAGATATAGCTTAGGTGCTCTAGGGCTGCCTATTACAAAGACATGGAGACCTTGGTACCATCAGAAGCAGGTGACTGGCTGGTATCAAGAATATGAGGGGCTTACATATGCAACACTTAGAGGAGCTGGGCATGATGTTCCCTCCTTCAAACCAGCAGAGTCATTGGTTCTATTCACATCTTTTCTCCTTGGGGAATCTCCGCCTTCGACTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

500

Amino Acids

56.38

Weight (kDa)

5.43

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 68 - 492 1.8e-139 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19640 AT5G45130 AT5G45130
fragaria_vesca FvH4_3g11910
malus_domestica MD05G1256700.v1.1 MD10G1237200.v1.1
prunus_persica Prupe.4G107100_v2.0.a1 Prupe.4G107100_v2.0.a1
pyrus_communis pycom05g23600 pycom10g19850
rosa_chinensis RchiOBHm_Chr5g0019441
rosa_laevigata RLG00000032443
rosa_multiflora Rmu_sc0002759.1_g000043
rosa_roxburghii Rroxscaffold_1G00058620 Rroxscaffold_1G00074300
rosa_rugosa Rorug05G0051000
rosa_samantha Rh5CG151800
rosa_wichuraiana Rw5G012560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1345
Acc36I ACCTGC 2 cut(s) 950, 1345
Acc65I GGTACC 1 cut(s) 1341
AccB1I GGYRCC 1 cut(s) 1341
AccI GTMKAC 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 1 cut(s) 1487
AclWI GGATC 4 cut(s) 139, 170, 192, 683
AcoI YGGCCR 4 cut(s) 21, 46, 77, 205
AcsI RAATTY 2 cut(s) 587, 1076
AdeI CACNNNGTG 1 cut(s) 881
AfaI GTAC 8 cut(s) 169, 617, 830, 874, 905, 1247, 1272, 1343
AfiI CCNNNNNNNGG 2 cut(s) 128, 469
AgsI TTSAA 6 cut(s) 226, 984, 992, 1084, 1185, 1435
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 4 cut(s) 48, 202, 346, 414
AjuI GAANNNNNNNTTGG 4 cut(s) 449, 465, 481, 497
AluBI AGCT 6 cut(s) 529, 658, 662, 1132, 1296, 1412
AluI AGCT 6 cut(s) 529, 658, 662, 1132, 1296, 1412
Alw21I GWGCWC 2 cut(s) 356, 1306
Alw26I GTCTC 3 cut(s) 1130, 1248, 1327
AlwI GGATC 4 cut(s) 139, 170, 192, 683
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 6 cut(s) 21, 46, 77, 205, 453, 1104
ApeKI GCWGC 3 cut(s) 296, 1223, 1312
ApoI RAATTY 2 cut(s) 587, 1076
AseI ATTAAT 1 cut(s) 818
Asp700I GAANNNNTTC 1 cut(s) 1076
Asp718I GGTACC 1 cut(s) 1341
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 4 cut(s) 344, 389, 609, 1105
AsuHPI GGTGA 1 cut(s) 1369
AsuII TTCGAA 2 cut(s) 968, 1070
AsuNHI GCTAGC 1 cut(s) 658
AvaII GGWCC 3 cut(s) 344, 389, 609
BaeGI GKGCMC 1 cut(s) 274
BaeI ACNNNNGTAYC 2 cut(s) 1325, 1358
BalI TGGCCA 2 cut(s) 48, 207
BanI GGYRCC 1 cut(s) 1341
Bbv12I GWGCWC 2 cut(s) 356, 1306
BbvCI CCTCAGC 2 cut(s) 117, 750
BbvI GCAGC 3 cut(s) 283, 1235, 1299
BccI CCATC 7 cut(s) 601, 718, 1055, 1115, 1198, 1256, 1353
BceAI ACGGC 2 cut(s) 8, 200
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BciT130I CCWGG 4 cut(s) 50, 204, 348, 416
BciVI GTATCC 1 cut(s) 728
BcoDI GTCTC 3 cut(s) 1130, 1248, 1327
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 5 cut(s) 135, 171, 530, 659, 1307
BfuAI ACCTGC 2 cut(s) 950, 1345
BfuI GTATCC 1 cut(s) 728
BisI GCNGC 3 cut(s) 297, 1224, 1313
BlsI GCNGC 3 cut(s) 298, 1225, 1314
BmcAI AGTACT 2 cut(s) 169, 830
Bme1390I CCNGG 4 cut(s) 50, 204, 348, 416
Bme18I GGWCC 3 cut(s) 344, 389, 609
BmgT120I GGNCC 4 cut(s) 344, 389, 609, 1105
BmiI GGNNCC 3 cut(s) 114, 373, 1343
BmrFI CCNGG 4 cut(s) 50, 204, 348, 416
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 3 cut(s) 736, 1039, 1155
BmtI GCTAGC 1 cut(s) 662
BmuI ACTGGG 1 cut(s) 168
BpmI CTGGAG 3 cut(s) 291, 1112, 1162
Bpu10I CCTNAGC 3 cut(s) 117, 750, 1297
Bpu14I TTCGAA 2 cut(s) 968, 1070
BpuEI CTTGAG 2 cut(s) 695, 1118
BsaBI GATNNNNATC 1 cut(s) 183
BsaI GGTCTC 2 cut(s) 1130, 1327
BsaJI CCNNGG 5 cut(s) 347, 415, 1028, 1337, 1474
BsaWI WCCGGW 2 cut(s) 606, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 469
Bse118I RCCGGY 2 cut(s) 19, 236
Bse1I ACTGG 7 cut(s) 7, 49, 163, 287, 1116, 1145, 1366
Bse3DI GCAATG 3 cut(s) 143, 618, 1218
Bse8I GATNNNNATC 1 cut(s) 183
BseAI TCCGGA 1 cut(s) 652
BseBI CCWGG 4 cut(s) 50, 204, 348, 416
BseDI CCNNGG 5 cut(s) 347, 415, 1028, 1337, 1474
BseGI GGATG 3 cut(s) 892, 1066, 1267
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 128, 469
BseMI GCAATG 3 cut(s) 143, 618, 1218
BseMII CTCAG 4 cut(s) 131, 654, 699, 741
BseNI ACTGG 7 cut(s) 7, 49, 163, 287, 1116, 1145, 1366
BseRI GAGGAG 1 cut(s) 1422
BseSI GKGCMC 1 cut(s) 274
BseX3I CGGCCG 1 cut(s) 21
BseXI GCAGC 3 cut(s) 283, 1235, 1299
BseYI CCCAGC 1 cut(s) 1412
BsgI GTGCAG 1 cut(s) 928
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 6 cut(s) 23, 48, 79, 207, 455, 1106
BshNI GGYRCC 1 cut(s) 1341
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 356, 1306
BsiSI CCGG 5 cut(s) 20, 76, 237, 607, 653
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 128, 469
BsmAI GTCTC 3 cut(s) 1130, 1248, 1327
BsmFI GGGAC 1 cut(s) 432
BsnI GGCC 6 cut(s) 23, 48, 79, 207, 455, 1106
Bso31I GGTCTC 2 cut(s) 1130, 1327
Bsp119I TTCGAA 2 cut(s) 968, 1070
Bsp1286I GDGCHC 3 cut(s) 274, 356, 1306
Bsp13I TCCGGA 1 cut(s) 652
Bsp1407I TGTACA 3 cut(s) 615, 872, 1245
Bsp143I GATC 6 cut(s) 131, 162, 184, 310, 688, 706
BspACI CCGC 1 cut(s) 1487
BspANI GGCC 6 cut(s) 23, 48, 79, 207, 455, 1106
BspCNI CTCAG 4 cut(s) 130, 655, 698, 742
BspEI TCCGGA 1 cut(s) 652
BspHI TCATGA 1 cut(s) 996
BspLI GGNNCC 3 cut(s) 114, 373, 1343
BspMI ACCTGC 2 cut(s) 950, 1345
BspOI GCTAGC 1 cut(s) 662
BspPI GGATC 4 cut(s) 139, 170, 192, 683
BspQI GCTCTTC 1 cut(s) 361
BspT104I TTCGAA 2 cut(s) 968, 1070
BspT107I GGYRCC 1 cut(s) 1341
BspTNI GGTCTC 2 cut(s) 1130, 1327
BsrDI GCAATG 3 cut(s) 143, 618, 1218
BsrFI RCCGGY 2 cut(s) 19, 236
BsrGI TGTACA 3 cut(s) 615, 872, 1245
BsrI ACTGG 7 cut(s) 7, 49, 163, 287, 1116, 1145, 1366
BssAI RCCGGY 2 cut(s) 19, 236
BssECI CCNNGG 5 cut(s) 347, 415, 1028, 1337, 1474
BssMI GATC 6 cut(s) 131, 162, 184, 310, 688, 706
BssT1I CCWWGG 3 cut(s) 1028, 1337, 1474
Bst2UI CCWGG 4 cut(s) 50, 204, 348, 416
Bst4CI ACNGT 5 cut(s) 250, 833, 845, 903, 1250
Bst6I CTCTTC 3 cut(s) 361, 1200, 1261
BstAUI TGTACA 3 cut(s) 615, 872, 1245
BstBI TTCGAA 2 cut(s) 968, 1070
BstC8I GCNNGC 7 cut(s) 81, 209, 213, 238, 376, 625, 660
BstDEI CTNAG 7 cut(s) 117, 663, 685, 750, 1232, 1297, 1403
BstF5I GGATG 3 cut(s) 892, 1066, 1267
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 6 cut(s) 134, 165, 187, 313, 691, 709
BstMAI GTCTC 3 cut(s) 1130, 1248, 1327
BstMBI GATC 6 cut(s) 131, 162, 184, 310, 688, 706
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 2 cut(s) 208, 1112
BstNI CCWGG 4 cut(s) 50, 204, 348, 416
BstNSI RCATGY 1 cut(s) 1056
BstSCI CCNGG 4 cut(s) 48, 202, 346, 414
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 3 cut(s) 283, 1235, 1299
BstX2I RGATCY 3 cut(s) 131, 184, 688
BstXI CCANNNNNNTGG 1 cut(s) 386
BstYI RGATCY 3 cut(s) 131, 184, 688
BstZI CGGCCG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 728
BsuRI GGCC 6 cut(s) 23, 48, 79, 207, 455, 1106
BtsCI GGATG 3 cut(s) 892, 1066, 1267
BtsIMutI CAGTG 3 cut(s) 246, 1123, 1255
BveI ACCTGC 2 cut(s) 950, 1345
Cac8I GCNNGC 7 cut(s) 81, 209, 213, 238, 376, 625, 660
CciI TCATGA 1 cut(s) 996
CfoI GCGC 1 cut(s) 550
Cfr10I RCCGGY 2 cut(s) 19, 236
Cfr13I GGNCC 4 cut(s) 344, 389, 609, 1105
Csp6I GTAC 8 cut(s) 168, 616, 829, 873, 904, 1246, 1271, 1342
CspCI CAANNNNNGTGG 2 cut(s) 90, 125
CviAII CATG 8 cut(s) 12, 441, 788, 997, 1053, 1112, 1329, 1418
CviQI GTAC 8 cut(s) 168, 616, 829, 873, 904, 1246, 1271, 1342
DdeI CTNAG 7 cut(s) 117, 663, 685, 750, 1232, 1297, 1403
DpnI GATC 6 cut(s) 133, 164, 186, 312, 690, 708
DpnII GATC 6 cut(s) 131, 162, 184, 310, 688, 706
DraI TTTAAA 1 cut(s) 898
DraIII CACNNNGTG 1 cut(s) 881
EaeI YGGCCR 4 cut(s) 21, 46, 77, 205
EagI CGGCCG 1 cut(s) 21
Eam1104I CTCTTC 3 cut(s) 361, 1200, 1261
EarI CTCTTC 3 cut(s) 361, 1200, 1261
EciI GGCGGA 1 cut(s) 1476
EclXI CGGCCG 1 cut(s) 21
Eco130I CCWWGG 3 cut(s) 1028, 1337, 1474
Eco31I GGTCTC 2 cut(s) 1130, 1327
Eco47I GGWCC 3 cut(s) 344, 389, 609
Eco52I CGGCCG 1 cut(s) 21
EcoO109I RGGNCCY 2 cut(s) 344, 1105
EcoRII CCWGG 4 cut(s) 48, 202, 346, 414
EcoT14I CCWWGG 3 cut(s) 1028, 1337, 1474
EcoT22I ATGCAT 2 cut(s) 17, 1054
ErhI CCWWGG 3 cut(s) 1028, 1337, 1474
FaeI CATG 8 cut(s) 15, 444, 791, 1000, 1056, 1115, 1332, 1421
FaqI GGGAC 1 cut(s) 432
FatI CATG 8 cut(s) 11, 440, 787, 996, 1052, 1111, 1328, 1417
FauNDI CATATG 1 cut(s) 1393
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 297, 1224, 1313
FokI GGATG 3 cut(s) 899, 1073, 1274
Fsp4HI GCNGC 3 cut(s) 297, 1224, 1313
FspBI CTAG 5 cut(s) 135, 171, 530, 659, 1307
GlaI GCGC 1 cut(s) 549
GluI GCNGC 3 cut(s) 297, 1224, 1313
GsaI CCCAGC 1 cut(s) 1416
GsuI CTGGAG 3 cut(s) 291, 1112, 1162
HaeIII GGCC 6 cut(s) 23, 48, 79, 207, 455, 1106
HapII CCGG 5 cut(s) 20, 76, 237, 607, 653
HhaI GCGC 1 cut(s) 550
Hin1II CATG 8 cut(s) 15, 444, 791, 1000, 1056, 1115, 1332, 1421
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HinfI GANTC 9 cut(s) 420, 473, 554, 784, 980, 1181, 1445, 1481, 1495
HpaII CCGG 5 cut(s) 20, 76, 237, 607, 653
HphI GGTGA 1 cut(s) 1369
Hpy166II GTNNAC 4 cut(s) 220, 253, 875, 1284
Hpy188I TCNGA 8 cut(s) 42, 480, 517, 688, 802, 979, 1180, 1350
Hpy188III TCNNGA 9 cut(s) 135, 308, 653, 721, 734, 997, 1058, 1135, 1373
Hpy8I GTNNAC 4 cut(s) 220, 253, 875, 1284
HpyAV CCTTC 4 cut(s) 94, 702, 1441, 1500
HpyCH4III ACNGT 5 cut(s) 250, 833, 845, 903, 1250
HpyCH4IV ACGT 1 cut(s) 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 1112
HpyF3I CTNAG 7 cut(s) 117, 663, 685, 750, 1232, 1297, 1403
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 8 cut(s) 15, 444, 791, 1000, 1056, 1115, 1332, 1421
HspAI GCGC 1 cut(s) 548
Kpn2I TCCGGA 1 cut(s) 652
KpnI GGTACC 1 cut(s) 1345
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Kzo9I GATC 6 cut(s) 131, 162, 184, 310, 688, 706
LguI GCTCTTC 1 cut(s) 361
LmnI GCTCC 5 cut(s) 112, 371, 655, 1143, 1409
Lsp1109I GCAGC 3 cut(s) 283, 1235, 1299
LweI GCATC 3 cut(s) 736, 1039, 1155
MaeI CTAG 5 cut(s) 135, 171, 530, 659, 1307
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 3 cut(s) 190, 244, 1357
MalI GATC 6 cut(s) 133, 164, 186, 312, 690, 708
MboI GATC 6 cut(s) 131, 162, 184, 310, 688, 706
MboII GAAGA 8 cut(s) 79, 82, 150, 348, 1072, 1187, 1190, 1278
MfeI CAATTG 1 cut(s) 1171
MflI RGATCY 3 cut(s) 131, 184, 688
MhlI GDGCHC 3 cut(s) 274, 356, 1306
MlsI TGGCCA 2 cut(s) 48, 207
MluCI AATT 5 cut(s) 587, 815, 868, 1076, 1171
MluNI TGGCCA 2 cut(s) 48, 207
MlyI GAGTC 4 cut(s) 414, 793, 1454, 1489
Mox20I TGGCCA 2 cut(s) 48, 207
Mph1103I ATGCAT 2 cut(s) 17, 1054
MroI TCCGGA 1 cut(s) 652
MroNI GCCGGC 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 1076
MscI TGGCCA 2 cut(s) 48, 207
MseI TTAA 6 cut(s) 65, 336, 408, 429, 818, 897
MslI CAYNNNNRTG 2 cut(s) 1001, 1116
Msp20I TGGCCA 2 cut(s) 48, 207
MspI CCGG 5 cut(s) 20, 76, 237, 607, 653
MspR9I CCNGG 4 cut(s) 50, 204, 348, 416
MunI CAATTG 1 cut(s) 1171
MvaI CCWGG 4 cut(s) 50, 204, 348, 416
MwoI GCNNNNNNNGC 2 cut(s) 208, 1112
NaeI GCCGGC 1 cut(s) 238
NdeI CATATG 1 cut(s) 1393
NdeII GATC 6 cut(s) 131, 162, 184, 310, 688, 706
NgoMIV GCCGGC 1 cut(s) 236
NheI GCTAGC 1 cut(s) 658
NlaIII CATG 8 cut(s) 15, 444, 791, 1000, 1056, 1115, 1332, 1421
NlaIV GGNNCC 3 cut(s) 114, 373, 1343
NmuCI GTSAC 3 cut(s) 190, 244, 1357
NsiI ATGCAT 2 cut(s) 17, 1054
NspI RCATGY 1 cut(s) 1056
NspV TTCGAA 2 cut(s) 968, 1070
PagI TCATGA 1 cut(s) 996
PaqCI CACCTGC 1 cut(s) 1345
PciSI GCTCTTC 1 cut(s) 361
PdiI GCCGGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 1076
PfeI GAWTC 5 cut(s) 473, 554, 980, 1181, 1481
PkrI GCNGC 3 cut(s) 298, 1225, 1314
PleI GAGTC 4 cut(s) 414, 792, 1453, 1489
PpsI GAGTC 4 cut(s) 414, 792, 1453, 1489
PpuMI RGGWCCY 1 cut(s) 344
PshBI ATTAAT 1 cut(s) 818
Psp5II RGGWCCY 1 cut(s) 344
Psp6I CCWGG 4 cut(s) 48, 202, 346, 414
PspFI CCCAGC 1 cut(s) 1412
PspGI CCWGG 4 cut(s) 48, 202, 346, 414
PspN4I GGNNCC 3 cut(s) 114, 373, 1343
PspPI GGNCC 4 cut(s) 344, 389, 609, 1105
PspPPI RGGWCCY 1 cut(s) 344
PsuI RGATCY 3 cut(s) 131, 184, 688
RsaI GTAC 8 cut(s) 169, 617, 830, 874, 905, 1247, 1272, 1343
RsaNI GTAC 8 cut(s) 168, 616, 829, 873, 904, 1246, 1271, 1342
RseI CAYNNNNRTG 2 cut(s) 1001, 1116
SapI GCTCTTC 1 cut(s) 361
SaqAI TTAA 6 cut(s) 65, 336, 408, 429, 818, 897
SatI GCNGC 3 cut(s) 297, 1224, 1313
Sau3AI GATC 6 cut(s) 131, 162, 184, 310, 688, 706
Sau96I GGNCC 4 cut(s) 344, 389, 609, 1105
ScaI AGTACT 2 cut(s) 169, 830
SchI GAGTC 4 cut(s) 414, 793, 1454, 1489
ScrFI CCNGG 4 cut(s) 50, 204, 348, 416
SduI GDGCHC 3 cut(s) 274, 356, 1306
SfaNI GCATC 3 cut(s) 736, 1039, 1155
SfuI TTCGAA 2 cut(s) 968, 1070
SinI GGWCC 3 cut(s) 344, 389, 609
SmiMI CAYNNNNRTG 2 cut(s) 1001, 1116
SmlI CTYRAG 2 cut(s) 710, 1133
SmoI CTYRAG 2 cut(s) 710, 1133
SpeI ACTAGT 1 cut(s) 170
Sse9I AATT 5 cut(s) 587, 815, 868, 1076, 1171
SsiI CCGC 1 cut(s) 1487
SspI AATATT 1 cut(s) 1164
SspMI CTAG 5 cut(s) 135, 171, 530, 659, 1307
StyD4I CCNGG 4 cut(s) 48, 202, 346, 414
StyI CCWWGG 3 cut(s) 1028, 1337, 1474
TaaI ACNGT 5 cut(s) 250, 833, 845, 903, 1250
TaiI ACGT 1 cut(s) 462
TaqI TCGA 3 cut(s) 968, 1070, 1493
TasI AATT 5 cut(s) 587, 815, 868, 1076, 1171
TatI WGTACW 6 cut(s) 167, 615, 828, 872, 903, 1245
TfiI GAWTC 5 cut(s) 473, 554, 980, 1181, 1481
Tru1I TTAA 6 cut(s) 65, 336, 408, 429, 818, 897
Tru9I TTAA 6 cut(s) 65, 336, 408, 429, 818, 897
TscAI CASTG 3 cut(s) 253, 1123, 1255
TseFI GTSAC 3 cut(s) 190, 244, 1357
TseI GCWGC 3 cut(s) 296, 1223, 1312
Tsp45I GTSAC 3 cut(s) 190, 244, 1357
TspDTI ATGAA 7 cut(s) 17, 82, 486, 546, 658, 903, 1019
TspGWI ACGGA 2 cut(s) 279, 1274
TspRI CASTG 3 cut(s) 253, 1123, 1255
VpaK11BI GGWCC 3 cut(s) 344, 389, 609
VspI ATTAAT 1 cut(s) 818
XapI RAATTY 2 cut(s) 587, 1076
XbaI TCTAGA 1 cut(s) 134
XceI RCATGY 1 cut(s) 1056
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 1076
XspI CTAG 5 cut(s) 135, 171, 530, 659, 1307
ZrmI AGTACT 2 cut(s) 169, 830
Zsp2I ATGCAT 2 cut(s) 17, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.