MD10G1243400.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014603
Physical Location & Seq
Reverse (-)
33796202 .. 33797880
1679 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1243400.v1.1.491

Sequence Viewer

Length: 1608 bp
ATGGCCTCCATTTCTGCAATCTCCGTCCTCCTCTTCCTCACATTTTCTGCCTCACTTTCGGCAGCTCTATCTGCTTCCGTCATAGAAGACCTCAGGAACTTAAAACCACCGCATGATTTTAACTCCACGATCACCAAAAACTGCCATCACAACCCATCACTCAGATACTGCAGTTCCTTACCTATGGACCTTGATGAGATTTTCAAATCCACCATTGTTGCAAGCCATCTCTGCAACGAGTCCAAGAATCCAAACTGCATCGAATCATTCCCTAAAATAGACCTTCAAAACCGCCCAATGATCACGCCGCTCTACTTGTCATTCAACTTCTTTTGGAAGTATTGCCCTTTGAGCATCTTAACTATTGACTTATCCAACAGTTCCTTAGACAGCAATTTTCCCATTGATGTTCTTCAATGCACCCAAATCCAAGCTCTTGATATGAGCCATAATGACCTTTACGGCGATGTCCCAATAGAGAGCTTCTCTCCGCTTACCAACCTCACTTCTCTTAATTTATCATACAATCACTTCTCTGAGAGTAAAATCTCTGATGCCCTCTTCTTTAAGCGGTTCAATTCTTCGAGTTTTCTCCATTCGGGTCTACTCCCCGATAAGAAAAAGTTCAGGATCAAGGCTGTAATCTTGTTGGTTGGTTTTCCGATCTTTGTGATCCTGATGGTGTGTTGCTTGGGGTGGCTTTGTTTCCGGAGGCCTGATTTCCTCCCAAGAATGCTTCGAAGAAAGCACCGGTTTACATCAGCAATGCTTAAGGCAGCAACAAATGGATTTTCGAAAAGAAATTTGAAGGGGAAGAGTGAAGCAGTTGATATCTACAAAGGAACTCTGAGAGATGGTACTGATGTCAGGATTGAGATTTACCGAGGAGAAAACCACCCGAACTTGATTGAAGAATGCAAGGTTCTTGTTCAGTTATGCCACAAGAACTTGGTTAGTATATTGGGTTGGTGCAATAACAGAAGGTTGAGAGCCATCGTAACTGAATGGATGGAAGGTGAGAGCCTTGAGATGTGGCTGTTGGAGGCAAACAGTACACCATGGAATCACAGGCTGAAGATATTGATGGGGGTTGTGGAAGGCATTTGTTATCTGCAGGAGGAATGGCCTGAAGTTGGATATGACCTCAGGACAAGCAGTATCTTGCTGTCAAACAATCTGGAACCGCGAATTTCGAGGTTTAAGGTCGGGGATCAAAGCAGTAGAAGTAAGTCGATCCATGAAGCTGCACCATTTATGTACAAGTTCGGAGTGCTTCTACTAGAGATGCTCGTAAACAGGCGACCGCAGGAAGACTTTGAGACGGGGGAGGCTGGTTTTCTCGAGTATATCAGAATGCATTATCCTGAAAATTTGCAGAAGGTGATGGACAAGAAATTGGCGACACAAGATACTTTTGATCAAGCTAAACAGGCAATAGGCCTAGGGCTAATGTGTACAGACATACCCAGCAACCAACAACTAGGGTTGGGTCAAGTGTTTGATATCATTAGTCGAGCCTACCAGTCTTGCAGGGTTTTAGCATCACATAACCGCGAAAGATTACATGCCGAAAGAGGTAAAGGGCACAAGCGCATACTATCGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Pkinase_Tyr

Protein Analysis

536

Amino Acids

61.13

Weight (kDa)

8.7

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 278 - 405 9.6e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 298 - 411 8.3e-08 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014603)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20790
fragaria_vesca FvH4_3g11180
malus_domestica MD05G1265400.v1.1 MD10G1243400.v1.1
prunus_persica Prupe.4G100500_v2.0.a1
pyrus_communis pycom05g24940 pycom10g20360
rosa_chinensis RchiOBHm_Chr5g0018091
rosa_laevigata RLG00000032339
rosa_multiflora Rmu_sc0010280.1_g000019
rosa_roxburghii Rroxscaffold_1G00059530
rosa_rugosa Rorug05G0040400
rosa_samantha Rh5AG133100 Rh5BG131700 Rh5CG143700 Rh5DG133400
rosa_wichuraiana Rw5G011600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 310
AccI GTMKAC 1 cut(s) 604
AccII CGCG 2 cut(s) 1186, 1554
AccIII TCCGGA 1 cut(s) 708
AciI CCGC 8 cut(s) 110, 292, 308, 491, 571, 1184, 1304, 1552
AclWI GGATC 4 cut(s) 638, 667, 1218, 1228
AcsI RAATTY 3 cut(s) 802, 1188, 1369
AcuI CTGAAG 2 cut(s) 1094, 1149
AfaI GTAC 4 cut(s) 859, 1054, 1259, 1456
AfiI CCNNNNNNNGG 1 cut(s) 1133
AflII CTTAAG 1 cut(s) 770
AgeI ACCGGT 1 cut(s) 750
AgsI TTSAA 7 cut(s) 205, 287, 325, 416, 577, 808, 911
AluBI AGCT 5 cut(s) 65, 434, 483, 1244, 1424
AluI AGCT 5 cut(s) 65, 434, 483, 1244, 1424
Alw26I GTCTC 1 cut(s) 1313
AlwI GGATC 4 cut(s) 638, 667, 1218, 1228
AlwNI CAGNNNCTG 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 1340
Aor13HI TCCGGA 1 cut(s) 708
AoxI GGCC 4 cut(s) 3, 713, 1124, 1438
ApeKI GCWGC 3 cut(s) 62, 776, 1244
ApoI RAATTY 3 cut(s) 802, 1188, 1369
AsiGI ACCGGT 1 cut(s) 750
Asp700I GAANNNNTTC 1 cut(s) 623
AspA2I CCTAGG 1 cut(s) 1441
AspLEI GCGC 1 cut(s) 1593
AspS9I GGNCC 1 cut(s) 187
AsuHPI GGTGA 3 cut(s) 124, 1028, 1393
AsuII TTCGAA 2 cut(s) 739, 794
AvaI CYCGRG 1 cut(s) 1340
AvaII GGWCC 1 cut(s) 187
AvrII CCTAGG 1 cut(s) 1441
AxyI CCTNAGG 2 cut(s) 92, 1145
BaeGI GKGCMC 1 cut(s) 1587
BaeI ACNNNNGTAYC 4 cut(s) 157, 190, 849, 882
BbsI GAAGAC 2 cut(s) 93, 1317
BbvI GCAGC 3 cut(s) 74, 788, 1231
BccI CCATC 9 cut(s) 153, 163, 234, 673, 848, 1001, 1003, 1078, 1378
BceAI ACGGC 1 cut(s) 478
BclI TGATCA 2 cut(s) 300, 1417
BcoDI GTCTC 1 cut(s) 1313
BfaI CTAG 3 cut(s) 1280, 1442, 1481
BfmI CTRYAG 2 cut(s) 169, 1112
BfrI CTTAAG 1 cut(s) 770
BisI GCNGC 4 cut(s) 63, 308, 777, 1245
BlnI CCTAGG 1 cut(s) 1441
BlsI GCNGC 4 cut(s) 64, 309, 778, 1246
Bme18I GGWCC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 1340
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 1182
BmsI GCATC 5 cut(s) 267, 363, 544, 1275, 1550
BpiI GAAGAC 2 cut(s) 93, 1317
BplI GAGNNNNNCTC 4 cut(s) 470, 502, 472, 504
Bpu14I TTCGAA 2 cut(s) 739, 794
BpuEI CTTGAG 1 cut(s) 1046
BsaJI CCNNGG 3 cut(s) 883, 1058, 1441
BsaWI WCCGGW 2 cut(s) 708, 750
Bsc4I CCNNNNNNNGG 1 cut(s) 1133
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 1 cut(s) 1522
Bse21I CCTNAGG 2 cut(s) 92, 1145
Bse3DI GCAATG 1 cut(s) 771
BseAI TCCGGA 1 cut(s) 708
BseDI CCNNGG 3 cut(s) 883, 1058, 1441
BseGI GGATG 1 cut(s) 1014
BseLI CCNNNNNNNGG 1 cut(s) 1133
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 5 cut(s) 106, 175, 528, 839, 1159
BseNI ACTGG 1 cut(s) 1522
BseRI GAGGAG 2 cut(s) 20, 900
BseSI GKGCMC 1 cut(s) 1587
BseXI GCAGC 3 cut(s) 74, 788, 1231
BseYI CCCAGC 1 cut(s) 1466
BsgI GTGCAG 1 cut(s) 1230
Bsh1236I CGCG 2 cut(s) 1186, 1554
Bsh1285I CGRYCG 1 cut(s) 1304
BshFI GGCC 4 cut(s) 5, 715, 1126, 1440
BshTI ACCGGT 1 cut(s) 750
BsiEI CGRYCG 1 cut(s) 1304
BsiHKCI CYCGRG 1 cut(s) 1340
BsiSI CCGG 2 cut(s) 709, 751
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 1133
BsmAI GTCTC 1 cut(s) 1313
BsmBI CGTCTC 1 cut(s) 1313
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 3 cut(s) 738, 920, 1359
BsnI GGCC 4 cut(s) 5, 715, 1126, 1440
BsoBI CYCGRG 1 cut(s) 1340
Bsp119I TTCGAA 2 cut(s) 739, 794
Bsp1286I GDGCHC 1 cut(s) 1587
Bsp13I TCCGGA 1 cut(s) 708
Bsp1407I TGTACA 2 cut(s) 1257, 1454
Bsp143I GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
Bsp19I CCATGG 1 cut(s) 1058
BspACI CCGC 8 cut(s) 110, 292, 308, 491, 571, 1184, 1304, 1552
BspANI GGCC 4 cut(s) 5, 715, 1126, 1440
BspCNI CTCAG 5 cut(s) 105, 174, 529, 840, 1158
BspEI TCCGGA 1 cut(s) 708
BspFNI CGCG 2 cut(s) 1186, 1554
BspLI GGNNCC 1 cut(s) 1182
BspMAI CTGCAG 2 cut(s) 173, 1116
BspPI GGATC 4 cut(s) 638, 667, 1218, 1228
BspT104I TTCGAA 2 cut(s) 739, 794
BspTI CTTAAG 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 750
BsrGI TGTACA 2 cut(s) 1257, 1454
BsrI ACTGG 1 cut(s) 1522
BssAI RCCGGY 1 cut(s) 750
BssECI CCNNGG 3 cut(s) 883, 1058, 1441
BssMI GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
BssT1I CCWWGG 2 cut(s) 1058, 1441
Bst4CI ACNGT 2 cut(s) 380, 1052
Bst6I CTCTTC 3 cut(s) 38, 566, 809
BstAFI CTTAAG 1 cut(s) 770
BstAUI TGTACA 2 cut(s) 1257, 1454
BstBI TTCGAA 2 cut(s) 739, 794
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 6 cut(s) 92, 161, 385, 537, 848, 1145
BstDSI CCRYGG 1 cut(s) 1058
BstF5I GGATG 1 cut(s) 1014
BstFNI CGCG 2 cut(s) 1186, 1554
BstHHI GCGC 1 cut(s) 1593
BstKTI GATC 8 cut(s) 132, 303, 633, 666, 675, 1213, 1236, 1420
BstMAI GTCTC 1 cut(s) 1313
BstMBI GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
BstMCI CGRYCG 1 cut(s) 1304
BstMWI GCNNNNNNNGC 4 cut(s) 71, 231, 351, 1430
BstNSI RCATGY 1 cut(s) 1568
BstSFI CTRYAG 2 cut(s) 169, 1112
BstSLI GKGCMC 1 cut(s) 1587
BstUI CGCG 2 cut(s) 1186, 1554
BstV1I GCAGC 3 cut(s) 74, 788, 1231
BstV2I GAAGAC 2 cut(s) 93, 1317
Bsu36I CCTNAGG 2 cut(s) 92, 1145
BsuRI GGCC 4 cut(s) 5, 715, 1126, 1440
BtgI CCRYGG 1 cut(s) 1058
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 1 cut(s) 1014
Cac8I GCNNGC 1 cut(s) 223
CaiI CAGNNNCTG 1 cut(s) 168
CfoI GCGC 1 cut(s) 1593
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 1 cut(s) 187
Csp6I GTAC 4 cut(s) 858, 1053, 1258, 1455
CspAI ACCGGT 1 cut(s) 750
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 4 cut(s) 113, 1059, 1238, 1565
CviQI GTAC 4 cut(s) 858, 1053, 1258, 1455
DdeI CTNAG 6 cut(s) 92, 161, 385, 537, 848, 1145
DpnI GATC 8 cut(s) 131, 302, 632, 665, 674, 1212, 1235, 1419
DpnII GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
Eam1104I CTCTTC 3 cut(s) 38, 566, 809
EarI CTCTTC 3 cut(s) 38, 566, 809
Eco130I CCWWGG 2 cut(s) 1058, 1441
Eco147I AGGCCT 2 cut(s) 715, 1440
Eco32I GATATC 2 cut(s) 832, 1504
Eco47I GGWCC 1 cut(s) 187
Eco57I CTGAAG 2 cut(s) 1094, 1149
Eco81I CCTNAGG 2 cut(s) 92, 1145
Eco88I CYCGRG 1 cut(s) 1340
EcoRV GATATC 2 cut(s) 832, 1504
EcoT14I CCWWGG 2 cut(s) 1058, 1441
EcoT22I ATGCAT 1 cut(s) 1359
ErhI CCWWGG 2 cut(s) 1058, 1441
Esp3I CGTCTC 1 cut(s) 1313
FaeI CATG 4 cut(s) 116, 1062, 1241, 1568
FaqI GGGAC 1 cut(s) 455
FatI CATG 4 cut(s) 112, 1058, 1237, 1564
FbaI TGATCA 2 cut(s) 300, 1417
FblI GTMKAC 1 cut(s) 604
Fnu4HI GCNGC 4 cut(s) 63, 308, 777, 1245
FokI GGATG 1 cut(s) 1021
Fsp4HI GCNGC 4 cut(s) 63, 308, 777, 1245
FspBI CTAG 3 cut(s) 1280, 1442, 1481
GlaI GCGC 1 cut(s) 1592
GluI GCNGC 4 cut(s) 63, 308, 777, 1245
GsaI CCCAGC 1 cut(s) 1470
HaeIII GGCC 4 cut(s) 5, 715, 1126, 1440
HapII CCGG 2 cut(s) 709, 751
HhaI GCGC 1 cut(s) 1593
Hin1II CATG 4 cut(s) 116, 1062, 1241, 1568
Hin6I GCGC 1 cut(s) 1591
HinP1I GCGC 1 cut(s) 1591
HinfI GANTC 4 cut(s) 239, 247, 263, 1063
HpaII CCGG 2 cut(s) 709, 751
HphI GGTGA 3 cut(s) 124, 1028, 1393
Hpy166II GTNNAC 5 cut(s) 605, 756, 1055, 1294, 1455
Hpy188I TCNGA 7 cut(s) 164, 538, 553, 663, 849, 1268, 1352
Hpy8I GTNNAC 5 cut(s) 605, 756, 1055, 1294, 1455
HpyAV CCTTC 6 cut(s) 293, 802, 975, 1007, 1091, 1372
HpyCH4III ACNGT 2 cut(s) 380, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 231, 351, 1430
HpyF3I CTNAG 6 cut(s) 92, 161, 385, 537, 848, 1145
Hsp92II CATG 4 cut(s) 116, 1062, 1241, 1568
HspAI GCGC 1 cut(s) 1591
Kpn2I TCCGGA 1 cut(s) 708
Ksp22I TGATCA 2 cut(s) 300, 1417
Kzo9I GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
Lsp1109I GCAGC 3 cut(s) 74, 788, 1231
LweI GCATC 5 cut(s) 267, 363, 544, 1275, 1550
MaeI CTAG 3 cut(s) 1280, 1442, 1481
MaeIII GTNAC 1 cut(s) 997
MalI GATC 8 cut(s) 131, 302, 632, 665, 674, 1212, 1235, 1419
MbiI CCGCTC 1 cut(s) 310
MboI GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
MhlI GDGCHC 1 cut(s) 1587
MluCI AATT 7 cut(s) 394, 514, 577, 802, 1188, 1369, 1394
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 3 cut(s) 399, 1020, 1114
Mph1103I ATGCAT 1 cut(s) 1359
MroI TCCGGA 1 cut(s) 708
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 2 cut(s) 709, 751
Mva1269I GAATGC 3 cut(s) 738, 920, 1359
MvnI CGCG 2 cut(s) 1186, 1554
MwoI GCNNNNNNNGC 4 cut(s) 71, 231, 351, 1430
NcoI CCATGG 1 cut(s) 1058
NdeII GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
NlaIII CATG 4 cut(s) 116, 1062, 1241, 1568
NlaIV GGNNCC 1 cut(s) 1182
NsiI ATGCAT 1 cut(s) 1359
NspI RCATGY 1 cut(s) 1568
NspV TTCGAA 2 cut(s) 739, 794
PaeR7I CTCGAG 1 cut(s) 1340
PceI AGGCCT 2 cut(s) 715, 1440
PctI GAATGC 3 cut(s) 738, 920, 1359
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 247, 263, 1063
PinAI ACCGGT 1 cut(s) 750
PkrI GCNGC 4 cut(s) 64, 309, 778, 1246
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 1466
PspN4I GGNNCC 1 cut(s) 1182
PspPI GGNCC 1 cut(s) 187
PstI CTGCAG 2 cut(s) 173, 1116
PstNI CAGNNNCTG 1 cut(s) 168
RsaI GTAC 4 cut(s) 859, 1054, 1259, 1456
RsaNI GTAC 4 cut(s) 858, 1053, 1258, 1455
SaqAI TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
SatI GCNGC 4 cut(s) 63, 308, 777, 1245
Sau3AI GATC 8 cut(s) 129, 300, 630, 663, 672, 1210, 1233, 1417
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 1 cut(s) 248
SduI GDGCHC 1 cut(s) 1587
SfaNI GCATC 5 cut(s) 267, 363, 544, 1275, 1550
SfcI CTRYAG 2 cut(s) 169, 1112
Sfr274I CTCGAG 1 cut(s) 1340
SfuI TTCGAA 2 cut(s) 739, 794
SinI GGWCC 1 cut(s) 187
SlaI CTCGAG 1 cut(s) 1340
SmlI CTYRAG 3 cut(s) 770, 1025, 1340
SmoI CTYRAG 3 cut(s) 770, 1025, 1340
Sse9I AATT 7 cut(s) 394, 514, 577, 802, 1188, 1369, 1394
SseBI AGGCCT 2 cut(s) 715, 1440
SsiI CCGC 8 cut(s) 110, 292, 308, 491, 571, 1184, 1304, 1552
SspMI CTAG 3 cut(s) 1280, 1442, 1481
StuI AGGCCT 2 cut(s) 715, 1440
StyI CCWWGG 2 cut(s) 1058, 1441
TaaI ACNGT 2 cut(s) 380, 1052
TaqI TCGA 9 cut(s) 261, 584, 739, 794, 1193, 1232, 1341, 1513, 1601
TasI AATT 7 cut(s) 394, 514, 577, 802, 1188, 1369, 1394
TatI WGTACW 3 cut(s) 1052, 1257, 1454
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 3 cut(s) 247, 263, 1063
Tru1I TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
Tru9I TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
TseI GCWGC 3 cut(s) 62, 776, 1244
TspDTI ATGAA 1 cut(s) 1254
TspGWI ACGGA 2 cut(s) 13, 67
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 3 cut(s) 802, 1188, 1369
XceI RCATGY 1 cut(s) 1568
XhoI CTCGAG 1 cut(s) 1340
XmaJI CCTAGG 1 cut(s) 1441
XmiI GTMKAC 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 3 cut(s) 1280, 1442, 1481
Zsp2I ATGCAT 1 cut(s) 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.