pycom10g20360

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014603
Physical Location & Seq
Reverse (-)
23269193 .. 23270910
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20360.2

Sequence Viewer

Length: 1620 bp
ATGGCCTCCATTTCTGCAACCTCCGTCCTCCTCTTCCTCACATTTTCCGCCTCACTTTCGGCAGCTCTATCTGCTTCCGTCATAGAAGACCTCAGGAACTTAAAACCACCGCATGATTTTAACTCCACGATCACGAAAAACTGCCATCACAACCCATCACTCAGATACTGCAGTTCCCTACCTATGGACCTTGATGAGATTTTCAAATCCACCATTGTTGCAAGCCATCTCTGCAACGAGTCCAAGAATCCAAACTGCACCGAATCATTCCCTAAAATAAACCTTCAAAACCGCCCAAAGATCACGCCGCTCTACTTGTCATTCAACTTCTTTTGGAAGTATTGCCCTTTGAGCATCTTAACTATTGACTTATCCAACAGTTCCTTAGAGAGCAATTTTCCCATTGATGTTCTTCAATGCACCCAAATCCAAGCTCTTGATATAAGCCATAATGACCTTCACGGCGATGTCCCAATAGAGAGCTTCTCTCCGCTTACCAACCTCACTTCTCTTAATTTATCATACAATCACTTCTCTGAGAGTAAAATCTCTGATGCCCTCTTCTTTAAGCGGTTCAATTCTTCGAGTTTTCTCCATTCAGGTCTACTCCCCGATAAGAAAAAGTTCAGGATCAAGGCTGTAATCTTGTTGGTTGGTTTTCCGATCTTTGTGATCCTGATGGTGTGTTGCTTGGGGTGGTTTTGTTTCCGGAGGCCTGATTTCCTCCCAAGAATGCTTCGAAGAAAGCACCGGTTTACACCAGCAATGCTTAAGGCAGCAACAAATGGATTTTCGAAAAGAAATTTGAAGGGGAAGAGTGAAGCAGTTGATATCTACAAAGGAACTCTGAGAGATGGTACTGACGTCAGGATTGAGATTTACCGAGGAGAAAACCACCGGAACTTGATTGAAGAATGCAAGGTTCTTGTTCAGTTATGCCACAAGAACTTGGTTAGTATATTGGGTTGGTGCAATAACAGAAGGTTGAGAGCCATCGTAACGGAATGGATTGAAGGTGAGAGCCTTGAGATGTGGCTGTTGGAGTCAAACAGTACACCATGGAATCACAGGTTGAAAATAGTGATGGGGGTTGTGGAAGGCATGTGTTATCTGCAGGAGGAATGGCCTGAAGTTGGATATGACCTCAGGACAAGCAGTATCTTGCTGTCAAACAATCTGGAACCGCTAATTTCGAGGTTTAAGGTCGGGGATCAAAGCAGTAGAAAGATGTACAAGTTTGGAGTGCTTCTACTAGAGATGCTCGTAAACAGGCGGCCGCAGGAAGACTTTGAGACGGGGGAGGCTGGTTTTCTCGAGTATATCAGAATGCATTATCCTGAAAATTTGCAGAAGGTGATAGACCAGAAATTGGCGACACAAGATACTTTTGATCAAGCTAGACAGGCAATAGGCCTAGGGCTAATGTGTACAGATATACCCAGCAACCAACAACTAGGGTTGGGTCAAGTGTTTGATATCATTAGTCGAGCCTACCAGTCTTGCAGGGTTTTAGCATCTCATAACCGCGAAAGATTACATGCCGAAAGAGGTAAACGGCACAAGCGCATACAATCAAGACGAACCATTGAATTCATCGAACCTCCAAAATCCAATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Pkinase_Tyr

Protein Analysis

540

Amino Acids

61.82

Weight (kDa)

8.92

Isoelectric Point (pI)

54.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014603)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20790
fragaria_vesca FvH4_3g11180
malus_domestica MD05G1265400.v1.1 MD10G1243400.v1.1
prunus_persica Prupe.4G100500_v2.0.a1
pyrus_communis pycom05g24940 pycom10g20360
rosa_chinensis RchiOBHm_Chr5g0018091
rosa_laevigata RLG00000032339
rosa_multiflora Rmu_sc0010280.1_g000019
rosa_roxburghii Rroxscaffold_1G00059530
rosa_rugosa Rorug05G0040400
rosa_samantha Rh5AG133100 Rh5BG131700 Rh5CG143700 Rh5DG133400
rosa_wichuraiana Rw5G011600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 867
AccB7I CCANNNNNTGG 1 cut(s) 1369
AccBSI CCGCTC 1 cut(s) 310
AccI GTMKAC 1 cut(s) 604
AccII CGCG 1 cut(s) 1527
AccIII TCCGGA 1 cut(s) 708
AclWI GGATC 3 cut(s) 638, 667, 1218
AcoI YGGCCR 1 cut(s) 1274
AcsI RAATTY 3 cut(s) 802, 1342, 1589
AcuI CTGAAG 1 cut(s) 1149
AcyI GRCGYC 1 cut(s) 864
AfaI GTAC 4 cut(s) 859, 1054, 1232, 1429
AfiI CCNNNNNNNGG 3 cut(s) 184, 1133, 1369
AflII CTTAAG 1 cut(s) 770
AgeI ACCGGT 1 cut(s) 750
AjuI GAANNNNNNNTTGG 1 cut(s) 1597
AluBI AGCT 4 cut(s) 65, 434, 483, 1397
AluI AGCT 4 cut(s) 65, 434, 483, 1397
Alw26I GTCTC 1 cut(s) 1286
AlwI GGATC 3 cut(s) 638, 667, 1218
AlwNI CAGNNNCTG 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 1313
Aor13HI TCCGGA 1 cut(s) 708
AoxI GGCC 5 cut(s) 3, 713, 1124, 1274, 1411
ApeKI GCWGC 2 cut(s) 62, 776
ApoI RAATTY 3 cut(s) 802, 1342, 1589
AsiGI ACCGGT 1 cut(s) 750
Asp700I GAANNNNTTC 1 cut(s) 623
AspA2I CCTAGG 1 cut(s) 1414
AspLEI GCGC 1 cut(s) 1566
AspS9I GGNCC 1 cut(s) 187
AsuHPI GGTGA 2 cut(s) 1028, 1366
AsuII TTCGAA 2 cut(s) 739, 794
AvaI CYCGRG 1 cut(s) 1313
AvaII GGWCC 1 cut(s) 187
AvrII CCTAGG 1 cut(s) 1414
AxyI CCTNAGG 2 cut(s) 92, 1145
BaeI ACNNNNGTAYC 4 cut(s) 157, 190, 849, 882
BbsI GAAGAC 2 cut(s) 93, 1290
BbvI GCAGC 2 cut(s) 74, 788
BccI CCATC 7 cut(s) 153, 163, 234, 673, 848, 1001, 1078
BceAI ACGGC 2 cut(s) 478, 1571
BclI TGATCA 1 cut(s) 1390
BcoDI GTCTC 1 cut(s) 1286
BfaI CTAG 4 cut(s) 1253, 1398, 1415, 1454
BfmI CTRYAG 2 cut(s) 169, 1112
BfrI CTTAAG 1 cut(s) 770
BisI GCNGC 5 cut(s) 63, 308, 777, 1274, 1277
BlnI CCTAGG 1 cut(s) 1414
BlsI GCNGC 5 cut(s) 64, 309, 778, 1275, 1278
Bme18I GGWCC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 1313
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 1182
BmsI GCATC 4 cut(s) 363, 544, 1248, 1523
BpiI GAAGAC 2 cut(s) 93, 1290
BplI GAGNNNNNCTC 4 cut(s) 470, 502, 472, 504
Bpu14I TTCGAA 2 cut(s) 739, 794
BpuEI CTTGAG 1 cut(s) 1046
BsaHI GRCGYC 1 cut(s) 864
BsaJI CCNNGG 3 cut(s) 883, 1058, 1414
BsaWI WCCGGW 3 cut(s) 708, 750, 897
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 1133, 1369
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 1 cut(s) 1495
Bse21I CCTNAGG 2 cut(s) 92, 1145
Bse3DI GCAATG 1 cut(s) 771
BseAI TCCGGA 1 cut(s) 708
BseDI CCNNGG 3 cut(s) 883, 1058, 1414
BseLI CCNNNNNNNGG 3 cut(s) 184, 1133, 1369
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 5 cut(s) 106, 175, 528, 839, 1159
BseNI ACTGG 1 cut(s) 1495
BseRI GAGGAG 2 cut(s) 20, 900
BseX3I CGGCCG 1 cut(s) 1274
BseXI GCAGC 2 cut(s) 74, 788
BseYI CCCAGC 1 cut(s) 1439
BsgI GTGCAG 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 1527
Bsh1285I CGRYCG 1 cut(s) 1277
BshFI GGCC 5 cut(s) 5, 715, 1126, 1276, 1413
BshTI ACCGGT 1 cut(s) 750
BsiEI CGRYCG 1 cut(s) 1277
BsiHKCI CYCGRG 1 cut(s) 1313
BsiSI CCGG 3 cut(s) 709, 751, 898
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 3 cut(s) 184, 1133, 1369
BsmAI GTCTC 1 cut(s) 1286
BsmBI CGTCTC 1 cut(s) 1286
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 3 cut(s) 738, 920, 1332
BsnI GGCC 5 cut(s) 5, 715, 1126, 1276, 1413
BsoBI CYCGRG 1 cut(s) 1313
Bsp119I TTCGAA 2 cut(s) 739, 794
Bsp13I TCCGGA 1 cut(s) 708
Bsp1407I TGTACA 2 cut(s) 1230, 1427
Bsp143I GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
Bsp19I CCATGG 1 cut(s) 1058
BspANI GGCC 5 cut(s) 5, 715, 1126, 1276, 1413
BspCNI CTCAG 5 cut(s) 105, 174, 529, 840, 1158
BspEI TCCGGA 1 cut(s) 708
BspFNI CGCG 1 cut(s) 1527
BspLI GGNNCC 1 cut(s) 1182
BspMAI CTGCAG 2 cut(s) 173, 1116
BspPI GGATC 3 cut(s) 638, 667, 1218
BspT104I TTCGAA 2 cut(s) 739, 794
BspTI CTTAAG 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 750
BsrGI TGTACA 2 cut(s) 1230, 1427
BsrI ACTGG 1 cut(s) 1495
BssAI RCCGGY 1 cut(s) 750
BssECI CCNNGG 3 cut(s) 883, 1058, 1414
BssMI GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
BssNI GRCGYC 1 cut(s) 864
BssT1I CCWWGG 2 cut(s) 1058, 1414
Bst4CI ACNGT 2 cut(s) 380, 1052
Bst6I CTCTTC 3 cut(s) 38, 566, 809
BstACI GRCGYC 1 cut(s) 864
BstAFI CTTAAG 1 cut(s) 770
BstAUI TGTACA 2 cut(s) 1230, 1427
BstBI TTCGAA 2 cut(s) 739, 794
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 6 cut(s) 92, 161, 385, 537, 848, 1145
BstDSI CCRYGG 1 cut(s) 1058
BstFNI CGCG 1 cut(s) 1527
BstHHI GCGC 1 cut(s) 1566
BstKTI GATC 7 cut(s) 132, 303, 633, 666, 675, 1213, 1393
BstMAI GTCTC 1 cut(s) 1286
BstMBI GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
BstMCI CGRYCG 1 cut(s) 1277
BstMWI GCNNNNNNNGC 4 cut(s) 71, 231, 351, 1403
BstNSI RCATGY 2 cut(s) 1105, 1541
BstSFI CTRYAG 2 cut(s) 169, 1112
BstUI CGCG 1 cut(s) 1527
BstV1I GCAGC 2 cut(s) 74, 788
BstV2I GAAGAC 2 cut(s) 93, 1290
BstZI CGGCCG 1 cut(s) 1274
Bsu36I CCTNAGG 2 cut(s) 92, 1145
BsuRI GGCC 5 cut(s) 5, 715, 1126, 1276, 1413
BtgI CCRYGG 1 cut(s) 1058
BtgZI GCGATG 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 223
CaiI CAGNNNCTG 1 cut(s) 168
CciNI GCGGCCGC 1 cut(s) 1274
CfoI GCGC 1 cut(s) 1566
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 1 cut(s) 187
Csp6I GTAC 4 cut(s) 858, 1053, 1231, 1428
CspAI ACCGGT 1 cut(s) 750
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 4 cut(s) 113, 1059, 1102, 1538
CviQI GTAC 4 cut(s) 858, 1053, 1231, 1428
DdeI CTNAG 6 cut(s) 92, 161, 385, 537, 848, 1145
DpnI GATC 7 cut(s) 131, 302, 632, 665, 674, 1212, 1392
DpnII GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
EaeI YGGCCR 1 cut(s) 1274
EagI CGGCCG 1 cut(s) 1274
Eam1104I CTCTTC 3 cut(s) 38, 566, 809
EarI CTCTTC 3 cut(s) 38, 566, 809
EciI GGCGGA 1 cut(s) 37
EclXI CGGCCG 1 cut(s) 1274
Eco130I CCWWGG 2 cut(s) 1058, 1414
Eco147I AGGCCT 2 cut(s) 715, 1413
Eco32I GATATC 2 cut(s) 832, 1477
Eco47I GGWCC 1 cut(s) 187
Eco52I CGGCCG 1 cut(s) 1274
Eco57I CTGAAG 1 cut(s) 1149
Eco81I CCTNAGG 2 cut(s) 92, 1145
Eco88I CYCGRG 1 cut(s) 1313
EcoRI GAATTC 1 cut(s) 1589
EcoRV GATATC 2 cut(s) 832, 1477
EcoT14I CCWWGG 2 cut(s) 1058, 1414
EcoT22I ATGCAT 1 cut(s) 1332
ErhI CCWWGG 2 cut(s) 1058, 1414
Esp3I CGTCTC 1 cut(s) 1286
FaeI CATG 4 cut(s) 116, 1062, 1105, 1541
FaqI GGGAC 1 cut(s) 455
FatI CATG 4 cut(s) 112, 1058, 1101, 1537
FbaI TGATCA 1 cut(s) 1390
FblI GTMKAC 1 cut(s) 604
Fnu4HI GCNGC 5 cut(s) 63, 308, 777, 1274, 1277
Fsp4HI GCNGC 5 cut(s) 63, 308, 777, 1274, 1277
FspBI CTAG 4 cut(s) 1253, 1398, 1415, 1454
GlaI GCGC 1 cut(s) 1565
GluI GCNGC 5 cut(s) 63, 308, 777, 1274, 1277
GsaI CCCAGC 1 cut(s) 1443
HaeIII GGCC 5 cut(s) 5, 715, 1126, 1276, 1413
HapII CCGG 3 cut(s) 709, 751, 898
HhaI GCGC 1 cut(s) 1566
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 4 cut(s) 116, 1062, 1105, 1541
Hin6I GCGC 1 cut(s) 1564
HinP1I GCGC 1 cut(s) 1564
HinfI GANTC 5 cut(s) 239, 247, 263, 1043, 1063
HpaII CCGG 3 cut(s) 709, 751, 898
HphI GGTGA 2 cut(s) 1028, 1366
Hpy166II GTNNAC 6 cut(s) 605, 756, 1055, 1267, 1428, 1553
Hpy188I TCNGA 6 cut(s) 164, 538, 553, 663, 849, 1325
Hpy8I GTNNAC 6 cut(s) 605, 756, 1055, 1267, 1428, 1553
HpyAV CCTTC 7 cut(s) 293, 467, 802, 975, 1007, 1091, 1345
HpyCH4III ACNGT 2 cut(s) 380, 1052
HpyCH4IV ACGT 1 cut(s) 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 231, 351, 1403
HpyF3I CTNAG 6 cut(s) 92, 161, 385, 537, 848, 1145
HpySE526I ACGT 1 cut(s) 864
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 4 cut(s) 116, 1062, 1105, 1541
HspAI GCGC 1 cut(s) 1564
Kpn2I TCCGGA 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 1390
Kzo9I GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
Lsp1109I GCAGC 2 cut(s) 74, 788
LweI GCATC 4 cut(s) 363, 544, 1248, 1523
MaeI CTAG 4 cut(s) 1253, 1398, 1415, 1454
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 1 cut(s) 997
MalI GATC 7 cut(s) 131, 302, 632, 665, 674, 1212, 1392
MbiI CCGCTC 1 cut(s) 310
MboI GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
MboII GAAGA 9 cut(s) 25, 98, 404, 553, 573, 753, 826, 923, 1295
MluCI AATT 9 cut(s) 394, 514, 577, 802, 1188, 1342, 1367, 1589, 1612
MlyI GAGTC 2 cut(s) 248, 1052
MmeI TCCRAC 3 cut(s) 399, 1020, 1114
Mph1103I ATGCAT 1 cut(s) 1332
MroI TCCGGA 1 cut(s) 708
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
MslI CAYNNNNRTG 1 cut(s) 465
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 3 cut(s) 709, 751, 898
Mva1269I GAATGC 3 cut(s) 738, 920, 1332
MvnI CGCG 1 cut(s) 1527
MwoI GCNNNNNNNGC 4 cut(s) 71, 231, 351, 1403
NcoI CCATGG 1 cut(s) 1058
NdeII GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
NlaIII CATG 4 cut(s) 116, 1062, 1105, 1541
NlaIV GGNNCC 1 cut(s) 1182
NotI GCGGCCGC 1 cut(s) 1274
NsiI ATGCAT 1 cut(s) 1332
NspI RCATGY 2 cut(s) 1105, 1541
NspV TTCGAA 2 cut(s) 739, 794
PaeR7I CTCGAG 1 cut(s) 1313
PceI AGGCCT 2 cut(s) 715, 1413
PctI GAATGC 3 cut(s) 738, 920, 1332
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 247, 263, 1063
PflMI CCANNNNNTGG 1 cut(s) 1369
PinAI ACCGGT 1 cut(s) 750
PkrI GCNGC 5 cut(s) 64, 309, 778, 1275, 1278
PleI GAGTC 2 cut(s) 247, 1051
PpsI GAGTC 2 cut(s) 247, 1051
PspFI CCCAGC 1 cut(s) 1439
PspN4I GGNNCC 1 cut(s) 1182
PspPI GGNCC 1 cut(s) 187
PstI CTGCAG 2 cut(s) 173, 1116
PstNI CAGNNNCTG 1 cut(s) 168
RsaI GTAC 4 cut(s) 859, 1054, 1232, 1429
RsaNI GTAC 4 cut(s) 858, 1053, 1231, 1428
RseI CAYNNNNRTG 1 cut(s) 465
SaqAI TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
SatI GCNGC 5 cut(s) 63, 308, 777, 1274, 1277
Sau3AI GATC 7 cut(s) 129, 300, 630, 663, 672, 1210, 1390
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 2 cut(s) 248, 1052
SfaNI GCATC 4 cut(s) 363, 544, 1248, 1523
SfcI CTRYAG 2 cut(s) 169, 1112
Sfr274I CTCGAG 1 cut(s) 1313
SfuI TTCGAA 2 cut(s) 739, 794
SinI GGWCC 1 cut(s) 187
SlaI CTCGAG 1 cut(s) 1313
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 3 cut(s) 770, 1025, 1313
SmoI CTYRAG 3 cut(s) 770, 1025, 1313
Sse9I AATT 9 cut(s) 394, 514, 577, 802, 1188, 1342, 1367, 1589, 1612
SseBI AGGCCT 2 cut(s) 715, 1413
SspMI CTAG 4 cut(s) 1253, 1398, 1415, 1454
StuI AGGCCT 2 cut(s) 715, 1413
StyI CCWWGG 2 cut(s) 1058, 1414
TaaI ACNGT 2 cut(s) 380, 1052
TaiI ACGT 1 cut(s) 867
TaqI TCGA 7 cut(s) 584, 739, 794, 1193, 1314, 1486, 1596
TasI AATT 9 cut(s) 394, 514, 577, 802, 1188, 1342, 1367, 1589, 1612
TatI WGTACW 3 cut(s) 1052, 1230, 1427
TauI GCSGC 3 cut(s) 310, 1276, 1279
TfiI GAWTC 3 cut(s) 247, 263, 1063
Tru1I TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
Tru9I TTAA 7 cut(s) 101, 120, 359, 513, 567, 771, 1200
TseI GCWGC 2 cut(s) 62, 776
TspDTI ATGAA 1 cut(s) 1582
TspGWI ACGGA 3 cut(s) 13, 67, 1016
Van91I CCANNNNNTGG 1 cut(s) 1369
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 3 cut(s) 802, 1342, 1589
XceI RCATGY 2 cut(s) 1105, 1541
XhoI CTCGAG 1 cut(s) 1313
XmaJI CCTAGG 1 cut(s) 1414
XmiI GTMKAC 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 4 cut(s) 1253, 1398, 1415, 1454
ZraI GACGTC 1 cut(s) 865
Zsp2I ATGCAT 1 cut(s) 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.