Rh5BG131700

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014603
Physical Location & Seq
Reverse (-)
12972641 .. 12974326
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG131700.1

Sequence Viewer

Length: 1602 bp
ATGGCTTCCATTTCTGCAACTTTCGTCATCCTACTATTCACGATTTCATTCTCAGTTTCTGCAACTCTATCTGCATCAGTTATAGATGACCTTAAACGTTTAAAACCACCTCAAGATTTCAACTCCACAATCATGAAAAACTGTCTTCAGAACCCATCTCTCAGATACTGCAGTACTTCTTCTCCAATGGACCTTGATGAGATTTTCAAATCCACCATTGTTGCAAGCCATCTCTGCAACGAGTCCAAGAACTCGAACTGCATCGAGTCATTCCCTAAAGTAGATCTTCGAAGCCGCCCAAACATTGCACCACTCTACTTGTCATTCACCTTCTTTTGGAAGTATTGCCCTTTAAGCATCTTATCTATTGACCTGTCCAATGCCTCTTTGAAGAGCAATTTTCCCATTGATGTCCTTCACTGCACCCAAATCCAATCTCTTGATATGAGCCATAATGAACTGTATGGTGATGTGCCAATAGAGAACTTCTCTGCCCTCACAAATCTCACAGTTCTGAATCTGTCATATAATCACTTCTCAGAGAGTAAAATCTCTGATACGCTGTTCTTCAAAAAGTTTAACTCTTCCAGTTTTCTTCATTCGGGTCTCATTCCAGATCACAAAAAGTTCAGAATCAAGGCTATCGTCTTGTTGATTGGTTTTCCTATCTTTGTGATTCTGAAGGTGTGTTGCTTGTGGTGGCTCTGTTTTCGCAGGCCTGATTTCCTCCCAAGAATGTTTCGAAGAAAGCACAGGTTTACACCAGCAATACTCAAGGCAGCAACCAATGGATTTTCGAGACACAATTTGATGGGGAAGAGCGCATCAGTTGATATTTACAAAGGAACTCTGAGAGATGGTACTGATGTCAGGATTGAGGTTTACAGTAGCGAAAGCCACCCGCAAGTGATTGAAGAATGCAAGGTTCTTGTTCAGCTATGCCATAAGAACCTGGCTAATTTACTTGGTTGGTGTGATAACCGAAAGTTGAGGGCCATTGTTACTGAATGGATGGAAGGAGAGAACCTAGAGAGCTGGCTTTTGGGATCAAGTTTTACACCATGGAACTACAGGTTGAGAATATTGATGGGGGTTGTGGAAGGCATGTGTTATCTACAGGAGCAGTGGCCTGAAGTTGGGTATGATCTGAGGACAAGTAGTATCATGTTGTGTAACTACAGTCTGGACCCAAAGATTTCAAGGTTTAAAGTTGGAAATCAAAGCAGTAGCAGTAGAAGGATGTACAAGTTTGGAGTATTCCTGCTAGAAATGATGGTAAACAGGAGGCCACAAGAGTTTGAGAGAGGCGAGGCTGGTTTTATAGAGTATATCAGAATGCATTATCCTGGAAACTTAGAGCAACTAATAGACCCGAAAATGGAATTGACTCAAAACACTGTTGACCAAGCTAAACAGGCAATATCCCTGGGGCTAATGTGCACAGAAGTATCTAGCAACCGGCAACCAACCTTAGAACAGGTGTTTGATATTGTAAGTAGAGCCTACCAGCCGTGCAGAGTTTTAGCATCTCATCATAATCATTATCACAGAAGATTTCATGCAGAAAGAGGAAAAGGACACAGGCGTGTGCAATCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Pkinase_Tyr

Protein Analysis

533

Amino Acids

61.39

Weight (kDa)

9.05

Isoelectric Point (pI)

53.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 269 - 392 6.8e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 295 - 413 1.3e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014603)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20790
fragaria_vesca FvH4_3g11180
malus_domestica MD05G1265400.v1.1 MD10G1243400.v1.1
prunus_persica Prupe.4G100500_v2.0.a1
pyrus_communis pycom05g24940 pycom10g20360
rosa_chinensis RchiOBHm_Chr5g0018091
rosa_laevigata RLG00000032339
rosa_multiflora Rmu_sc0010280.1_g000019
rosa_roxburghii Rroxscaffold_1G00059530
rosa_rugosa Rorug05G0040400
rosa_samantha Rh5AG133100 Rh5BG131700 Rh5CG143700 Rh5DG133400
rosa_wichuraiana Rw5G011600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 295, 902
AclI AACGTT 1 cut(s) 97
AclWI GGATC 1 cut(s) 1054
AcuI CTGAAG 3 cut(s) 131, 701, 1152
AfaI GTAC 3 cut(s) 175, 862, 1244
AfiI CCNNNNNNNGG 3 cut(s) 336, 1136, 1378
AgsI TTSAA 6 cut(s) 121, 208, 391, 571, 914, 1200
AjnI CCWGG 3 cut(s) 951, 1345, 1425
AleI CACNNNNGTG 1 cut(s) 1584
AluBI AGCT 3 cut(s) 937, 1035, 1409
AluI AGCT 3 cut(s) 937, 1035, 1409
Alw21I GWGCWC 1 cut(s) 1442
Alw26I GTCTC 2 cut(s) 611, 793
Alw44I GTGCAC 1 cut(s) 1438
AlwI GGATC 1 cut(s) 1054
AlwNI CAGNNNCTG 2 cut(s) 59, 168
AoxI GGCC 4 cut(s) 716, 993, 1127, 1286
ApaLI GTGCAC 1 cut(s) 1438
ApeKI GCWGC 1 cut(s) 779
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 3 cut(s) 190, 993, 1186
AsuHPI GGTGA 2 cut(s) 319, 479
AsuII TTCGAA 2 cut(s) 289, 742
AvaII GGWCC 2 cut(s) 190, 1186
BaeGI GKGCMC 1 cut(s) 1442
BaeI ACNNNNGTAYC 6 cut(s) 157, 190, 852, 885, 1431, 1464
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 1442
BbvI GCAGC 1 cut(s) 791
BccI CCATC 7 cut(s) 163, 237, 805, 851, 1006, 1081, 1267
BceAI ACGGC 1 cut(s) 1495
BciT130I CCWGG 3 cut(s) 953, 1347, 1427
BcoDI GTCTC 2 cut(s) 611, 793
BfaI CTAG 3 cut(s) 1028, 1265, 1452
BfmI CTRYAG 4 cut(s) 169, 1069, 1115, 1177
BglII AGATCT 1 cut(s) 283
BisI GCNGC 2 cut(s) 295, 780
BlsI GCNGC 2 cut(s) 296, 781
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 3 cut(s) 953, 1347, 1427
Bme18I GGWCC 2 cut(s) 190, 1186
BmgT120I GGNCC 3 cut(s) 190, 993, 1186
BmiI GGNNCC 1 cut(s) 1188
BmrFI CCNGG 3 cut(s) 953, 1347, 1427
BmsI GCATC 5 cut(s) 83, 270, 366, 833, 1535
BpiI GAAGAC 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 473, 505
Bpu14I TTCGAA 2 cut(s) 289, 742
BpuEI CTTGAG 2 cut(s) 96, 758
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 3 cut(s) 1061, 1425, 1426
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 3 cut(s) 336, 1136, 1378
Bse118I RCCGGY 1 cut(s) 1458
Bse1I ACTGG 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 3 cut(s) 953, 1347, 1427
BseDI CCNNGG 3 cut(s) 1061, 1425, 1426
BseGI GGATG 3 cut(s) 27, 1017, 1245
BseLI CCNNNNNNNGG 3 cut(s) 336, 1136, 1378
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 5 cut(s) 66, 175, 552, 842, 1139
BseNI ACTGG 1 cut(s) 588
BseSI GKGCMC 1 cut(s) 1442
BseXI GCAGC 1 cut(s) 791
BsgI GTGCAG 2 cut(s) 406, 1534
BshFI GGCC 4 cut(s) 718, 995, 1129, 1288
BsiHKAI GWGCWC 1 cut(s) 1442
BsiSI CCGG 1 cut(s) 1459
BslI CCNNNNNNNGG 3 cut(s) 336, 1136, 1378
BsmAI GTCTC 2 cut(s) 611, 793
BsmI GAATGC 2 cut(s) 923, 1341
BsnI GGCC 4 cut(s) 718, 995, 1129, 1288
Bso31I GGTCTC 1 cut(s) 611
Bsp119I TTCGAA 2 cut(s) 289, 742
Bsp1286I GDGCHC 1 cut(s) 1442
Bsp1407I TGTACA 1 cut(s) 1242
Bsp143I GATC 4 cut(s) 283, 616, 1046, 1144
Bsp19I CCATGG 1 cut(s) 1061
BspACI CCGC 2 cut(s) 295, 902
BspANI GGCC 4 cut(s) 718, 995, 1129, 1288
BspCNI CTCAG 5 cut(s) 65, 174, 551, 843, 1140
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 1188
BspMAI CTGCAG 1 cut(s) 173
BspPI GGATC 1 cut(s) 1054
BspQI GCTCTTC 2 cut(s) 386, 812
BspT104I TTCGAA 2 cut(s) 289, 742
BspTNI GGTCTC 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 1458
BsrGI TGTACA 1 cut(s) 1242
BsrI ACTGG 1 cut(s) 588
BssAI RCCGGY 1 cut(s) 1458
BssECI CCNNGG 3 cut(s) 1061, 1425, 1426
BssMI GATC 4 cut(s) 283, 616, 1046, 1144
BssT1I CCWWGG 1 cut(s) 1061
Bst2UI CCWGG 3 cut(s) 953, 1347, 1427
Bst4CI ACNGT 6 cut(s) 143, 462, 511, 887, 1181, 1399
Bst6I CTCTTC 3 cut(s) 386, 589, 812
BstAUI TGTACA 1 cut(s) 1242
BstBI TTCGAA 2 cut(s) 289, 742
BstC8I GCNNGC 3 cut(s) 226, 716, 1037
BstDEI CTNAG 7 cut(s) 52, 161, 538, 851, 1148, 1354, 1471
BstDSI CCRYGG 1 cut(s) 1061
BstF5I GGATG 3 cut(s) 27, 1017, 1245
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 4 cut(s) 286, 619, 1049, 1147
BstMAI GTCTC 2 cut(s) 611, 793
BstMBI GATC 4 cut(s) 283, 616, 1046, 1144
BstMWI GCNNNNNNNGC 3 cut(s) 234, 354, 1415
BstNI CCWGG 3 cut(s) 953, 1347, 1427
BstNSI RCATGY 1 cut(s) 1108
BstSCI CCNGG 3 cut(s) 951, 1345, 1425
BstSFI CTRYAG 4 cut(s) 169, 1069, 1115, 1177
BstSLI GKGCMC 1 cut(s) 1442
BstV1I GCAGC 1 cut(s) 791
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 4 cut(s) 718, 995, 1129, 1288
BtgI CCRYGG 1 cut(s) 1061
BtsCI GGATG 3 cut(s) 27, 1017, 1245
BtsI GCAGTG 2 cut(s) 418, 1130
BtsIMutI CAGTG 3 cut(s) 418, 1130, 1395
Cac8I GCNNGC 3 cut(s) 226, 716, 1037
CaiI CAGNNNCTG 2 cut(s) 59, 168
CciI TCATGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 824
Cfr10I RCCGGY 1 cut(s) 1458
Cfr13I GGNCC 3 cut(s) 190, 993, 1186
Csp6I GTAC 3 cut(s) 174, 861, 1243
CspCI CAANNNNNGTGG 4 cut(s) 202, 237, 300, 335
CviAII CATG 5 cut(s) 133, 1062, 1105, 1165, 1559
CviQI GTAC 3 cut(s) 174, 861, 1243
DdeI CTNAG 7 cut(s) 52, 161, 538, 851, 1148, 1354, 1471
DpnI GATC 4 cut(s) 285, 618, 1048, 1146
DpnII GATC 4 cut(s) 283, 616, 1046, 1144
DraI TTTAAA 2 cut(s) 102, 1207
Eam1104I CTCTTC 3 cut(s) 386, 589, 812
EarI CTCTTC 3 cut(s) 386, 589, 812
Eco130I CCWWGG 1 cut(s) 1061
Eco147I AGGCCT 1 cut(s) 718
Eco31I GGTCTC 1 cut(s) 611
Eco47I GGWCC 2 cut(s) 190, 1186
Eco57I CTGAAG 3 cut(s) 131, 701, 1152
EcoRII CCWGG 3 cut(s) 951, 1345, 1425
EcoT14I CCWWGG 1 cut(s) 1061
EcoT22I ATGCAT 1 cut(s) 1341
ErhI CCWWGG 1 cut(s) 1061
FaeI CATG 5 cut(s) 136, 1065, 1108, 1168, 1562
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 5 cut(s) 132, 1061, 1104, 1164, 1558
FauI CCCGC 1 cut(s) 909
Fnu4HI GCNGC 2 cut(s) 295, 780
FokI GGATG 3 cut(s) 14, 1024, 1252
Fsp4HI GCNGC 2 cut(s) 295, 780
FspBI CTAG 3 cut(s) 1028, 1265, 1452
GlaI GCGC 1 cut(s) 823
GluI GCNGC 2 cut(s) 295, 780
HaeIII GGCC 4 cut(s) 718, 995, 1129, 1288
HapII CCGG 1 cut(s) 1459
HhaI GCGC 1 cut(s) 824
Hin1II CATG 5 cut(s) 136, 1065, 1108, 1168, 1562
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 1 cut(s) 1402
HindII GTYRAC 1 cut(s) 1402
HinfI GANTC 6 cut(s) 242, 266, 517, 633, 676, 1387
HpaII CCGG 1 cut(s) 1459
HphI GGTGA 2 cut(s) 319, 479
Hpy166II GTNNAC 5 cut(s) 759, 883, 1279, 1402, 1440
Hpy188III TCNNGA 9 cut(s) 40, 113, 133, 440, 614, 798, 871, 1184, 1596
Hpy8I GTNNAC 5 cut(s) 759, 883, 1279, 1402, 1440
HpyAV CCTTC 6 cut(s) 340, 425, 676, 1010, 1094, 1230
HpyCH4III ACNGT 6 cut(s) 143, 462, 511, 887, 1181, 1399
HpyCH4IV ACGT 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 3 cut(s) 234, 354, 1415
HpyF3I CTNAG 7 cut(s) 52, 161, 538, 851, 1148, 1354, 1471
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 5 cut(s) 136, 1065, 1108, 1168, 1562
HspAI GCGC 1 cut(s) 822
Kzo9I GATC 4 cut(s) 283, 616, 1046, 1144
LguI GCTCTTC 2 cut(s) 386, 812
LmnI GCTCC 1 cut(s) 1120
Lsp1109I GCAGC 1 cut(s) 791
LweI GCATC 5 cut(s) 83, 270, 366, 833, 1535
MaeI CTAG 3 cut(s) 1028, 1265, 1452
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 2 cut(s) 1000, 1172
MalI GATC 4 cut(s) 285, 618, 1048, 1146
MboI GATC 4 cut(s) 283, 616, 1046, 1144
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 1442
MluCI AATT 4 cut(s) 397, 805, 958, 1382
MlyI GAGTC 3 cut(s) 251, 275, 1381
MmeI TCCRAC 1 cut(s) 1192
Mph1103I ATGCAT 1 cut(s) 1341
MseI TTAA 5 cut(s) 93, 101, 353, 579, 1206
MslI CAYNNNNRTG 2 cut(s) 131, 1584
MspI CCGG 1 cut(s) 1459
MspR9I CCNGG 3 cut(s) 953, 1347, 1427
Mva1269I GAATGC 2 cut(s) 923, 1341
MvaI CCWGG 3 cut(s) 953, 1347, 1427
MwoI GCNNNNNNNGC 3 cut(s) 234, 354, 1415
NcoI CCATGG 1 cut(s) 1061
NdeII GATC 4 cut(s) 283, 616, 1046, 1144
NlaIII CATG 5 cut(s) 136, 1065, 1108, 1168, 1562
NlaIV GGNNCC 1 cut(s) 1188
NsiI ATGCAT 1 cut(s) 1341
NspI RCATGY 1 cut(s) 1108
NspV TTCGAA 2 cut(s) 289, 742
OliI CACNNNNGTG 1 cut(s) 1584
PagI TCATGA 1 cut(s) 132
PasI CCCWGGG 1 cut(s) 1426
PceI AGGCCT 1 cut(s) 718
PciSI GCTCTTC 2 cut(s) 386, 812
PctI GAATGC 2 cut(s) 923, 1341
PfeI GAWTC 3 cut(s) 517, 633, 676
PfoI TCCNGGA 1 cut(s) 1345
PkrI GCNGC 2 cut(s) 296, 781
PleI GAGTC 3 cut(s) 250, 274, 1381
PpsI GAGTC 3 cut(s) 250, 274, 1381
Psp1406I AACGTT 1 cut(s) 97
Psp6I CCWGG 3 cut(s) 951, 1345, 1425
PspGI CCWGG 3 cut(s) 951, 1345, 1425
PspN4I GGNNCC 1 cut(s) 1188
PspPI GGNCC 3 cut(s) 190, 993, 1186
PstI CTGCAG 1 cut(s) 173
PstNI CAGNNNCTG 2 cut(s) 59, 168
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 3 cut(s) 175, 862, 1244
RsaNI GTAC 3 cut(s) 174, 861, 1243
RseI CAYNNNNRTG 2 cut(s) 131, 1584
SapI GCTCTTC 2 cut(s) 386, 812
SaqAI TTAA 5 cut(s) 93, 101, 353, 579, 1206
SatI GCNGC 2 cut(s) 295, 780
Sau3AI GATC 4 cut(s) 283, 616, 1046, 1144
Sau96I GGNCC 3 cut(s) 190, 993, 1186
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 3 cut(s) 251, 275, 1381
ScrFI CCNGG 3 cut(s) 953, 1347, 1427
SduI GDGCHC 1 cut(s) 1442
SfaNI GCATC 5 cut(s) 83, 270, 366, 833, 1535
SfcI CTRYAG 4 cut(s) 169, 1069, 1115, 1177
SfuI TTCGAA 2 cut(s) 289, 742
SinI GGWCC 2 cut(s) 190, 1186
SmiMI CAYNNNNRTG 2 cut(s) 131, 1584
SmlI CTYRAG 2 cut(s) 111, 773
SmoI CTYRAG 2 cut(s) 111, 773
Sse9I AATT 4 cut(s) 397, 805, 958, 1382
SseBI AGGCCT 1 cut(s) 718
SsiI CCGC 2 cut(s) 295, 902
SspI AATATT 1 cut(s) 1083
SspMI CTAG 3 cut(s) 1028, 1265, 1452
StuI AGGCCT 1 cut(s) 718
StyD4I CCNGG 3 cut(s) 951, 1345, 1425
StyI CCWWGG 1 cut(s) 1061
TaaI ACNGT 6 cut(s) 143, 462, 511, 887, 1181, 1399
TaiI ACGT 1 cut(s) 100
TaqI TCGA 5 cut(s) 254, 264, 289, 742, 797
TasI AATT 4 cut(s) 397, 805, 958, 1382
TatI WGTACW 2 cut(s) 173, 1242
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 3 cut(s) 517, 633, 676
Tru1I TTAA 5 cut(s) 93, 101, 353, 579, 1206
Tru9I TTAA 5 cut(s) 93, 101, 353, 579, 1206
TscAI CASTG 3 cut(s) 425, 1130, 1402
TseI GCWGC 1 cut(s) 779
TspDTI ATGAA 5 cut(s) 36, 149, 471, 587, 1547
TspRI CASTG 3 cut(s) 425, 1130, 1402
VneI GTGCAC 1 cut(s) 1438
VpaK11BI GGWCC 2 cut(s) 190, 1186
XceI RCATGY 1 cut(s) 1108
XspI CTAG 3 cut(s) 1028, 1265, 1452
ZrmI AGTACT 1 cut(s) 175
Zsp2I ATGCAT 1 cut(s) 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.