MD10G1296500.v1.1

Stem-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017260
Physical Location & Seq
Forward (+)
38425157 .. 38426889
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1296500.v1.1.491

Sequence Viewer

Length: 768 bp
ATGCTTGCAATATTCAACAAGGAAGTGGTGCAGCCACCGCAGGAGCTCCACAGCCCCGCCGCTGCCTCCGCCAATCCCGCCAAGAAGCCTCAAGAAATTGCCAAAGATTTCATGTCCTGCCATGATCCAAACAACGCAGTTTCAGTCTGTTTTGGAAACACAGCTTCGCTTGCTTACGTTCCTCCAGCCAACCCCTACACATCCCCAAAAAGGGTGTTCTGTTCATTGGACAACATATACTGCATTTTTCTGGGGGACCTGAACAACCTCTGCAGCCTGATCAAGCAGTACGGGCTGTCGAAGGGCACCAATGAGGCCATGTTCGTGGTGCAGGCCTATCGGACCCTCCGCGACCGCGGCCCGTACCCGGCTGATCAGGTCCTCAAGGATCTTGATGGAAGCTTTGGATTTGTGCTCTATGACACCAAGGCTGGAACCGTCTTTGCTGCATTGGGTGCTAATGAAGGGGTTAGCATGTTTTGGGGCATTGCAGCAGATGGTTCGGTGGTGATTTCTGACAACTTGGGGGTCGTAAAACAAAGCTGTGCTAAATCTTTTGCCCCATTTCCAACTGGGTGCATGTTCCACAGTGAGCAAGGGCTGATGAGCTTTGAGCATCCAACCAAGAAGATGAAGGCAATGCCTCGGATTGACAGTGAGGGGGTCATGTGTGGGGCCACCTTCAAGGTTGATGCCCAATCGAGGATTCCCAACATGCCACGTGTCGGAAGTGAAGCCAATTGGGCAGCATGGGGCCAACAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

256

Amino Acids

27.55

Weight (kDa)

6.88

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 230 4.6e-101 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017260)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g04880
malus_domestica MD05G1315200.v1.1 MD10G1296500.v1.1
prunus_persica Prupe.4G044100_v2.0.a1
pyrus_communis pycom05g29280
rosa_chinensis RchiOBHm_Chr5g0008041
rosa_laevigata RLG00000031530
rosa_multiflora Rmu_sc0007348.1_g000001
rosa_rugosa Rorug04G0432900
rosa_samantha Rh5AG060900 Rh5BG057700 Rh5DG056900
rosa_wichuraiana Rw5G005970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 305
AccII CGCG 2 cut(s) 351, 357
AciI CCGC 8 cut(s) 38, 57, 60, 69, 78, 349, 355, 357
AclWI GGATC 2 cut(s) 119, 396
AcvI CACGTG 1 cut(s) 722
AfaI GTAC 2 cut(s) 290, 365
AfiI CCNNNNNNNGG 5 cut(s) 210, 211, 367, 702, 725
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 2 cut(s) 16, 685
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
AluBI AGCT 5 cut(s) 46, 164, 402, 543, 609
AluI AGCT 5 cut(s) 46, 164, 402, 543, 609
Alw21I GWGCWC 2 cut(s) 48, 417
AlwI GGATC 2 cut(s) 119, 396
AoxI GGCC 5 cut(s) 315, 333, 358, 675, 754
ApeKI GCWGC 6 cut(s) 31, 62, 273, 446, 491, 746
AspS9I GGNCC 6 cut(s) 256, 342, 359, 379, 675, 754
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 520
AvaII GGWCC 3 cut(s) 256, 342, 379
BaeGI GKGCMC 1 cut(s) 308
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 722
Bbv12I GWGCWC 2 cut(s) 48, 417
BbvI GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
BccI CCATC 2 cut(s) 389, 491
BcgI CGANNNNNNTGC 4 cut(s) 320, 354, 483, 517
BclI TGATCA 2 cut(s) 279, 373
BcnI CCSGG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 271
BglI GCCNNNNNGGC 1 cut(s) 743
BisI GCNGC 8 cut(s) 32, 60, 63, 274, 358, 447, 492, 747
BlsI GCNGC 8 cut(s) 33, 61, 64, 275, 359, 448, 493, 748
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 3 cut(s) 256, 342, 379
BmgT120I GGNCC 6 cut(s) 256, 342, 359, 379, 675, 754
BmiI GGNNCC 6 cut(s) 257, 307, 344, 436, 676, 755
BmrFI CCNGG 1 cut(s) 368
BmrI ACTGGG 1 cut(s) 582
BmsI GCATC 2 cut(s) 625, 682
BmuI ACTGGG 1 cut(s) 582
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 2 cut(s) 75, 368
BpuMI CCSGG 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 722
BsaJI CCNNGG 3 cut(s) 355, 426, 644
Bsc4I CCNNNNNNNGG 5 cut(s) 210, 211, 367, 702, 725
Bse1I ACTGG 1 cut(s) 577
Bse3DI GCAATG 2 cut(s) 486, 645
BseDI CCNNGG 3 cut(s) 355, 426, 644
BseGI GGATG 2 cut(s) 200, 616
BseLI CCNNNNNNNGG 5 cut(s) 210, 211, 367, 702, 725
BseMI GCAATG 2 cut(s) 486, 645
BseNI ACTGG 1 cut(s) 577
BseSI GKGCMC 1 cut(s) 308
BseXI GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
BsgI GTGCAG 2 cut(s) 50, 350
Bsh1236I CGCG 2 cut(s) 351, 357
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 5 cut(s) 317, 335, 360, 677, 756
BshNI GGYRCC 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 2 cut(s) 48, 417
BsiSI CCGG 1 cut(s) 368
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 5 cut(s) 210, 211, 367, 702, 725
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 5 cut(s) 317, 335, 360, 677, 756
Bsp1286I GDGCHC 3 cut(s) 48, 308, 417
Bsp143I GATC 4 cut(s) 124, 279, 373, 388
BspACI CCGC 8 cut(s) 38, 57, 60, 69, 78, 349, 355, 357
BspANI GGCC 5 cut(s) 317, 335, 360, 677, 756
BspFNI CGCG 2 cut(s) 351, 357
BspLI GGNNCC 6 cut(s) 257, 307, 344, 436, 676, 755
BspMAI CTGCAG 1 cut(s) 275
BspPI GGATC 2 cut(s) 119, 396
BspT107I GGYRCC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 486, 645
BsrI ACTGG 1 cut(s) 577
BssECI CCNNGG 3 cut(s) 355, 426, 644
BssMI GATC 4 cut(s) 124, 279, 373, 388
BssT1I CCWWGG 1 cut(s) 426
Bst4CI ACNGT 3 cut(s) 439, 590, 656
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 3 cut(s) 6, 171, 333
BstDSI CCRYGG 1 cut(s) 355
BstF5I GGATG 2 cut(s) 200, 616
BstFNI CGCG 2 cut(s) 351, 357
BstKTI GATC 4 cut(s) 127, 282, 376, 391
BstMBI GATC 4 cut(s) 124, 279, 373, 388
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 8 cut(s) 37, 68, 77, 170, 292, 357, 455, 743
BstNSI RCATGY 3 cut(s) 478, 583, 718
BstSCI CCNGG 1 cut(s) 366
BstSFI CTRYAG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 308
BstUI CGCG 2 cut(s) 351, 357
BstV1I GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 1 cut(s) 325
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 5 cut(s) 317, 335, 360, 677, 756
BtgI CCRYGG 1 cut(s) 355
BtsCI GGATG 2 cut(s) 200, 616
BtsIMutI CAGTG 2 cut(s) 595, 661
Cac8I GCNNGC 3 cut(s) 6, 171, 333
Cfr13I GGNCC 6 cut(s) 256, 342, 359, 379, 675, 754
Cfr42I CCGCGG 1 cut(s) 358
Csp6I GTAC 2 cut(s) 289, 364
CviAII CATG 8 cut(s) 112, 122, 319, 475, 580, 667, 715, 750
CviQI GTAC 2 cut(s) 289, 364
DpnI GATC 4 cut(s) 126, 281, 375, 390
DpnII GATC 4 cut(s) 124, 279, 373, 388
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 48
Eco47I GGWCC 3 cut(s) 256, 342, 379
Eco53kI GAGCTC 1 cut(s) 46
Eco72I CACGTG 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 2 cut(s) 256, 379
EcoT14I CCWWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 8 cut(s) 115, 125, 322, 478, 583, 670, 718, 753
FaqI GGGAC 1 cut(s) 269
FatI CATG 8 cut(s) 111, 121, 318, 474, 579, 666, 714, 749
FauI CCCGC 2 cut(s) 64, 85
FbaI TGATCA 2 cut(s) 279, 373
Fnu4HI GCNGC 8 cut(s) 32, 60, 63, 274, 358, 447, 492, 747
FokI GGATG 2 cut(s) 187, 603
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 8 cut(s) 32, 60, 63, 274, 358, 447, 492, 747
GluI GCNGC 8 cut(s) 32, 60, 63, 274, 358, 447, 492, 747
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 5 cut(s) 317, 335, 360, 677, 756
HapII CCGG 1 cut(s) 368
Hin1II CATG 8 cut(s) 115, 125, 322, 478, 583, 670, 718, 753
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 1 cut(s) 706
HpaII CCGG 1 cut(s) 368
HphI GGTGA 1 cut(s) 520
Hpy188I TCNGA 4 cut(s) 342, 517, 648, 728
Hpy188III TCNNGA 2 cut(s) 92, 392
HpyAV CCTTC 4 cut(s) 295, 458, 628, 691
HpyCH4III ACNGT 3 cut(s) 439, 590, 656
HpyCH4IV ACGT 2 cut(s) 177, 721
HpyCH4V TGCA 8 cut(s) 8, 31, 243, 273, 331, 449, 491, 579
HpyF10VI GCNNNNNNNGC 8 cut(s) 37, 68, 77, 170, 292, 357, 455, 743
HpySE526I ACGT 2 cut(s) 177, 721
Hsp92II CATG 8 cut(s) 115, 125, 322, 478, 583, 670, 718, 753
Ksp22I TGATCA 2 cut(s) 279, 373
KspI CCGCGG 1 cut(s) 358
Kzo9I GATC 4 cut(s) 124, 279, 373, 388
LmnI GCTCC 2 cut(s) 43, 51
Lsp1109I GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
LweI GCATC 2 cut(s) 625, 682
MaeII ACGT 2 cut(s) 177, 721
MalI GATC 4 cut(s) 126, 281, 375, 390
MboI GATC 4 cut(s) 124, 279, 373, 388
MboII GAAGA 1 cut(s) 640
MfeI CAATTG 1 cut(s) 739
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 3 cut(s) 48, 308, 417
MluCI AATT 2 cut(s) 96, 739
MmeI TCCRAC 3 cut(s) 593, 644, 706
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 2 cut(s) 62, 357
MspI CCGG 1 cut(s) 368
MspR9I CCNGG 1 cut(s) 368
MunI CAATTG 1 cut(s) 739
MvnI CGCG 2 cut(s) 351, 357
MwoI GCNNNNNNNGC 8 cut(s) 37, 68, 77, 170, 292, 357, 455, 743
NciI CCSGG 1 cut(s) 368
NdeII GATC 4 cut(s) 124, 279, 373, 388
NlaIII CATG 8 cut(s) 115, 125, 322, 478, 583, 670, 718, 753
NlaIV GGNNCC 6 cut(s) 257, 307, 344, 436, 676, 755
NspI RCATGY 3 cut(s) 478, 583, 718
PceI AGGCCT 1 cut(s) 335
PfeI GAWTC 1 cut(s) 706
PkrI GCNGC 8 cut(s) 33, 61, 64, 275, 359, 448, 493, 748
PmaCI CACGTG 1 cut(s) 722
PmlI CACGTG 1 cut(s) 722
Ppu21I YACGTR 1 cut(s) 722
PpuMI RGGWCCY 2 cut(s) 256, 379
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 2 cut(s) 256, 379
PspCI CACGTG 1 cut(s) 722
PspN4I GGNNCC 6 cut(s) 257, 307, 344, 436, 676, 755
PspPI GGNCC 6 cut(s) 256, 342, 359, 379, 675, 754
PspPPI RGGWCCY 2 cut(s) 256, 379
PstI CTGCAG 1 cut(s) 275
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 2 cut(s) 290, 365
RsaNI GTAC 2 cut(s) 289, 364
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 358
SatI GCNGC 8 cut(s) 32, 60, 63, 274, 358, 447, 492, 747
Sau3AI GATC 4 cut(s) 124, 279, 373, 388
Sau96I GGNCC 6 cut(s) 256, 342, 359, 379, 675, 754
ScrFI CCNGG 1 cut(s) 368
SduI GDGCHC 3 cut(s) 48, 308, 417
SfaNI GCATC 2 cut(s) 625, 682
SfcI CTRYAG 1 cut(s) 271
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 3 cut(s) 256, 342, 379
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 90, 383
SmoI CTYRAG 2 cut(s) 90, 383
Sse9I AATT 2 cut(s) 96, 739
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 8 cut(s) 38, 57, 60, 69, 78, 349, 355, 357
SspI AATATT 1 cut(s) 12
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 3 cut(s) 439, 590, 656
TaiI ACGT 2 cut(s) 180, 724
TaqI TCGA 2 cut(s) 299, 701
TasI AATT 2 cut(s) 96, 739
TauI GCSGC 2 cut(s) 62, 360
TfiI GAWTC 1 cut(s) 706
TscAI CASTG 2 cut(s) 595, 661
TseI GCWGC 6 cut(s) 31, 62, 273, 446, 491, 746
TspDTI ATGAA 4 cut(s) 100, 213, 477, 647
TspRI CASTG 2 cut(s) 595, 661
VpaK11BI GGWCC 3 cut(s) 256, 342, 379
XceI RCATGY 3 cut(s) 478, 583, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.