pycom05g29280

Stem-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0017260
Physical Location & Seq
Forward (+)
29744256 .. 29745613
1358 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29280.3

Sequence Viewer

Length: 768 bp
ATGCTGGCAATATTCAATAAGGAAGTAGTGCAGCCACCGCAGGAGCTCCACAGCCCCACCGCTGCCTCCGTCAATCCCGCCATGAAGCCTCAAGAAATTGCCAAAGACTTCATGTCCTTCCATGATCCAAACAATGCCTTTTCAGTCTGTTTTGGAAACACAGCTTTGCTTGCTTATGTTCCTCCAACCAACCCCTACTCATCTCAAAAACGGGTGTTCTGTTCTTTGGACAACATACACTGCATTTTCCTTGGGGGCCTGAACAACCTCTGCAGCCTGATCAAGCAGTACGGGCTGTCGAAGGGCACCAATGAGGCCATGTTTGTGGTGGAGGCCTATCGGACTCTTCGCGACCGCGGCCCTTACCCAGCTGATCAGGTCCTCAAGGATCTTGATGGAAGCTTTGGCTTTGTGATTTATGACACCAAGGCTGGAACCGTCTTTGCTGCATTGGGTGCTAATGAAGGGGTTAGCATGTTCTGGGGCATTGCAGCAGATGGTTCGGTGGTGATTTCTGACAACTTGGGGGTCATGAAACAAAGCTGTGCTAAATCTTTTGCCCCATTTCCAACTGGGTGCATGTTCCACAGTGAGCAAGGATTGATGAGCTTTGAGCATCCAACCAAGAAGATGAAGGCAATGCCTAGAGTTGATAGTGAGGGTGTTATGTGTGGGGCCACCTTCAAGGTTGACGTCCAATCAAGGATTCCCAACATGCCACGTGTTGGTAGTGAAGCTAATTGGGCAGCATGGGGCCAACCAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

256

Amino Acids

27.71

Weight (kDa)

6.51

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017260)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g04880
malus_domestica MD05G1315200.v1.1 MD10G1296500.v1.1
prunus_persica Prupe.4G044100_v2.0.a1
pyrus_communis pycom05g29280
rosa_chinensis RchiOBHm_Chr5g0008041
rosa_laevigata RLG00000031530
rosa_multiflora Rmu_sc0007348.1_g000001
rosa_rugosa Rorug04G0432900
rosa_samantha Rh5AG060900 Rh5BG057700 Rh5DG056900
rosa_wichuraiana Rw5G005970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 696
AccB1I GGYRCC 1 cut(s) 305
AccB7I CCANNNNNTGG 1 cut(s) 725
AccII CGCG 2 cut(s) 351, 357
AciI CCGC 5 cut(s) 38, 60, 78, 355, 357
AclWI GGATC 2 cut(s) 119, 396
AcvI CACGTG 1 cut(s) 722
AcyI GRCGYC 1 cut(s) 693
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 1 cut(s) 725
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 2 cut(s) 16, 685
AhdI GACNNNNNGTC 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
AluBI AGCT 7 cut(s) 46, 164, 371, 402, 543, 609, 737
AluI AGCT 7 cut(s) 46, 164, 371, 402, 543, 609, 737
Alw21I GWGCWC 1 cut(s) 48
AlwI GGATC 2 cut(s) 119, 396
AoxI GGCC 6 cut(s) 256, 315, 333, 358, 675, 754
ApeKI GCWGC 6 cut(s) 31, 62, 273, 446, 491, 746
AspS9I GGNCC 5 cut(s) 256, 359, 379, 675, 754
AsuHPI GGTGA 1 cut(s) 520
AvaII GGWCC 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 308
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 722
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
BccI CCATC 2 cut(s) 389, 491
BcgI CGANNNNNNTGC 2 cut(s) 483, 517
BclI TGATCA 2 cut(s) 279, 373
BfaI CTAG 2 cut(s) 645, 766
BfmI CTRYAG 1 cut(s) 271
BisI GCNGC 7 cut(s) 32, 63, 274, 358, 447, 492, 747
BlsI GCNGC 7 cut(s) 33, 64, 275, 359, 448, 493, 748
Bme18I GGWCC 1 cut(s) 379
BmeRI GACNNNNNGTC 1 cut(s) 112
BmgT120I GGNCC 5 cut(s) 256, 359, 379, 675, 754
BmiI GGNNCC 5 cut(s) 257, 307, 436, 676, 755
BmrI ACTGGG 1 cut(s) 582
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 75, 368
BsaAI YACGTR 1 cut(s) 722
BsaHI GRCGYC 1 cut(s) 693
BsaJI CCNNGG 3 cut(s) 250, 355, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse1I ACTGG 1 cut(s) 577
Bse3DI GCAATG 2 cut(s) 486, 645
BseDI CCNNGG 3 cut(s) 250, 355, 426
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 1 cut(s) 725
BseMI GCAATG 2 cut(s) 486, 645
BseNI ACTGG 1 cut(s) 577
BseSI GKGCMC 1 cut(s) 308
BseXI GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 50
Bsh1236I CGCG 2 cut(s) 351, 357
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 6 cut(s) 258, 317, 335, 360, 677, 756
BshNI GGYRCC 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 725
BsnI GGCC 6 cut(s) 258, 317, 335, 360, 677, 756
Bsp1286I GDGCHC 2 cut(s) 48, 308
Bsp143I GATC 4 cut(s) 124, 279, 373, 388
Bsp68I TCGCGA 1 cut(s) 351
BspACI CCGC 5 cut(s) 38, 60, 78, 355, 357
BspANI GGCC 6 cut(s) 258, 317, 335, 360, 677, 756
BspFNI CGCG 2 cut(s) 351, 357
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 5 cut(s) 257, 307, 436, 676, 755
BspMAI CTGCAG 1 cut(s) 275
BspPI GGATC 2 cut(s) 119, 396
BspT107I GGYRCC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 486, 645
BsrI ACTGG 1 cut(s) 577
BssECI CCNNGG 3 cut(s) 250, 355, 426
BssMI GATC 4 cut(s) 124, 279, 373, 388
BssNI GRCGYC 1 cut(s) 693
BssT1I CCWWGG 2 cut(s) 250, 426
Bst4CI ACNGT 2 cut(s) 439, 590
Bst6I CTCTTC 1 cut(s) 351
BstACI GRCGYC 1 cut(s) 693
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 2 cut(s) 6, 171
BstDSI CCRYGG 1 cut(s) 355
BstF5I GGATG 1 cut(s) 616
BstFNI CGCG 2 cut(s) 351, 357
BstKTI GATC 4 cut(s) 127, 282, 376, 391
BstMBI GATC 4 cut(s) 124, 279, 373, 388
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 6 cut(s) 37, 170, 292, 357, 455, 743
BstNSI RCATGY 3 cut(s) 478, 583, 718
BstSFI CTRYAG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 308
BstUI CGCG 2 cut(s) 351, 357
BstV1I GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 1 cut(s) 325
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 6 cut(s) 258, 317, 335, 360, 677, 756
BtgI CCRYGG 1 cut(s) 355
BtsCI GGATG 1 cut(s) 616
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 238, 595
BtuMI TCGCGA 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 6, 171
CciI TCATGA 1 cut(s) 531
Cfr13I GGNCC 5 cut(s) 256, 359, 379, 675, 754
Cfr42I CCGCGG 1 cut(s) 358
Csp6I GTAC 1 cut(s) 289
CviAII CATG 9 cut(s) 82, 112, 122, 319, 475, 532, 580, 715, 750
CviQI GTAC 1 cut(s) 289
DpnI GATC 4 cut(s) 126, 281, 375, 390
DpnII GATC 4 cut(s) 124, 279, 373, 388
DriI GACNNNNNGTC 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 351
Eam1105I GACNNNNNGTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 351
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 250, 426
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 46
Eco72I CACGTG 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 2 cut(s) 256, 379
EcoT14I CCWWGG 2 cut(s) 250, 426
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 250, 426
FaeI CATG 9 cut(s) 85, 115, 125, 322, 478, 535, 583, 718, 753
FatI CATG 9 cut(s) 81, 111, 121, 318, 474, 531, 579, 714, 749
FauI CCCGC 1 cut(s) 85
FbaI TGATCA 2 cut(s) 279, 373
Fnu4HI GCNGC 7 cut(s) 32, 63, 274, 358, 447, 492, 747
FokI GGATG 1 cut(s) 603
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 7 cut(s) 32, 63, 274, 358, 447, 492, 747
FspBI CTAG 2 cut(s) 645, 766
GluI GCNGC 7 cut(s) 32, 63, 274, 358, 447, 492, 747
GsaI CCCAGC 1 cut(s) 371
HaeIII GGCC 6 cut(s) 258, 317, 335, 360, 677, 756
Hin1I GRCGYC 1 cut(s) 693
Hin1II CATG 9 cut(s) 85, 115, 125, 322, 478, 535, 583, 718, 753
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 2 cut(s) 343, 706
HphI GGTGA 1 cut(s) 520
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 2 cut(s) 342, 517
Hpy188III TCNNGA 4 cut(s) 92, 350, 392, 532
Hpy8I GTNNAC 1 cut(s) 691
HpyAV CCTTC 5 cut(s) 127, 295, 458, 628, 691
HpyCH4III ACNGT 2 cut(s) 439, 590
HpyCH4IV ACGT 2 cut(s) 693, 721
HpyCH4V TGCA 6 cut(s) 31, 243, 273, 449, 491, 579
HpyF10VI GCNNNNNNNGC 6 cut(s) 37, 170, 292, 357, 455, 743
HpySE526I ACGT 2 cut(s) 693, 721
Hsp92I GRCGYC 1 cut(s) 693
Hsp92II CATG 9 cut(s) 85, 115, 125, 322, 478, 535, 583, 718, 753
Ksp22I TGATCA 2 cut(s) 279, 373
KspI CCGCGG 1 cut(s) 358
Kzo9I GATC 4 cut(s) 124, 279, 373, 388
LmnI GCTCC 2 cut(s) 43, 51
LpnPI CCDG 8 cut(s) 26, 272, 290, 362, 381, 417, 466, 558
Lsp1109I GCAGC 6 cut(s) 43, 49, 285, 433, 503, 758
LweI GCATC 1 cut(s) 625
MaeI CTAG 2 cut(s) 645, 766
MaeII ACGT 2 cut(s) 693, 721
MalI GATC 4 cut(s) 126, 281, 375, 390
MboI GATC 4 cut(s) 124, 279, 373, 388
MboII GAAGA 2 cut(s) 338, 640
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 48, 308
MluCI AATT 2 cut(s) 96, 739
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 3 cut(s) 209, 593, 644
MnlI CCTC 8 cut(s) 76, 99, 192, 278, 307, 325, 392, 652
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 3 cut(s) 62, 357, 371
MvnI CGCG 2 cut(s) 351, 357
MwoI GCNNNNNNNGC 6 cut(s) 37, 170, 292, 357, 455, 743
NdeII GATC 4 cut(s) 124, 279, 373, 388
NlaIII CATG 9 cut(s) 85, 115, 125, 322, 478, 535, 583, 718, 753
NlaIV GGNNCC 5 cut(s) 257, 307, 436, 676, 755
NruI TCGCGA 1 cut(s) 351
NspI RCATGY 3 cut(s) 478, 583, 718
PagI TCATGA 1 cut(s) 531
PceI AGGCCT 1 cut(s) 335
PfeI GAWTC 1 cut(s) 706
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 7 cut(s) 33, 64, 275, 359, 448, 493, 748
PleI GAGTC 1 cut(s) 337
PmaCI CACGTG 1 cut(s) 722
PmlI CACGTG 1 cut(s) 722
PpsI GAGTC 1 cut(s) 337
Ppu21I YACGTR 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 379
PspCI CACGTG 1 cut(s) 722
PspFI CCCAGC 1 cut(s) 367
PspN4I GGNNCC 5 cut(s) 257, 307, 436, 676, 755
PspPI GGNCC 5 cut(s) 256, 359, 379, 675, 754
PspPPI RGGWCCY 1 cut(s) 379
PstI CTGCAG 1 cut(s) 275
PsuI RGATCY 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 371
RruI TCGCGA 1 cut(s) 351
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 358
SatI GCNGC 7 cut(s) 32, 63, 274, 358, 447, 492, 747
Sau3AI GATC 4 cut(s) 124, 279, 373, 388
Sau96I GGNCC 5 cut(s) 256, 359, 379, 675, 754
SchI GAGTC 1 cut(s) 337
SduI GDGCHC 2 cut(s) 48, 308
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 271
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 90, 383
SmoI CTYRAG 2 cut(s) 90, 383
Sse9I AATT 2 cut(s) 96, 739
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 5 cut(s) 38, 60, 78, 355, 357
SspI AATATT 1 cut(s) 12
SspMI CTAG 2 cut(s) 645, 766
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 335
StyI CCWWGG 2 cut(s) 250, 426
TaaI ACNGT 2 cut(s) 439, 590
TaiI ACGT 2 cut(s) 696, 724
TaqI TCGA 1 cut(s) 299
TasI AATT 2 cut(s) 96, 739
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 1 cut(s) 706
TscAI CASTG 2 cut(s) 245, 595
TseI GCWGC 6 cut(s) 31, 62, 273, 446, 491, 746
TspDTI ATGAA 5 cut(s) 98, 100, 477, 548, 647
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 2 cut(s) 245, 595
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 379
XceI RCATGY 3 cut(s) 478, 583, 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XspI CTAG 2 cut(s) 645, 766
ZraI GACGTC 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.