MD10G1311900.v1.1

Thioredoxin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014533
Physical Location & Seq
Forward (+)
39653573 .. 39655729
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1311900.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGCAGGTCAACTACGAGAAGAGCAGCAGGTAACTAGGCCCAAAGTCATGAAGGTTGACTCTGTGGAGACTTGGGACTCGCATGTTTCCCAAGCCACCAACCAAGGCCGTCCTATTATTGCACATTTCACTGCTTCGTGGTGCATGCCTTCGGTGGCTATGAACTCATTCTTCGAGGAAATTGCCTCAGATTATCCGGATGTTCTGTTTCTCACCGTTGATGTCGACGAGGTTAAGGAGGTAGCGACCCGATTGGAGATAAAGGCGATGCCAACCTTTTCGCTGATAAGGGACGGTGCAACGGTTGACAAGCTCGTGGGTGCCAATCCGGAAGAGATAAGGAAAAGGATTGAGGGTTTCTTTCAGTCCAGACGTGCGGATGTTGCATAG

Protein Analysis

130

Amino Acids

14.53

Weight (kDa)

5.02

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 19 - 117 5.8e-20 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11530
fragaria_vesca FvH4_3g02911
malus_domestica MD05G1336400.v1.1 MD10G1311900.v1.1
prunus_persica Prupe.4G029100_v2.0.a1
pyrus_communis pycom05g30790 pycom10g26320
rosa_chinensis RchiOBHm_Chr5g0004481
rosa_laevigata RLG00000031232
rosa_multiflora Rmu_sc0000485.1_g000001
rosa_roxburghii Rroxscaffold_1G00071040
rosa_rugosa Rorug04G0412100
rosa_samantha Rh5AG039700 Rh5BG038700 Rh5CG042800 Rh5DG038500
rosa_wichuraiana Rw5G003790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 225
AccIII TCCGGA 2 cut(s) 196, 328
AciI CCGC 1 cut(s) 377
AjiI CACGTC 1 cut(s) 374
AluBI AGCT 1 cut(s) 313
AluI AGCT 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 62
Aor13HI TCCGGA 2 cut(s) 196, 328
AoxI GGCC 2 cut(s) 38, 106
ApeKI GCWGC 1 cut(s) 25
Asp700I GAANNNNTTC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 205
BanI GGYRCC 1 cut(s) 320
BauI CACGAG 1 cut(s) 314
BbvI GCAGC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 1 cut(s) 36
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
BmgBI CACGTC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 1 cut(s) 322
BmsI GCATC 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 2 cut(s) 196, 328
BseAI TCCGGA 2 cut(s) 196, 328
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 2 cut(s) 205, 385
BseMII CTCAG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 37
BshFI GGCC 2 cut(s) 40, 108
BshNI GGYRCC 1 cut(s) 320
BsiSI CCGG 2 cut(s) 197, 329
BslFI GGGAC 2 cut(s) 89, 305
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 2 cut(s) 89, 305
BsnI GGCC 2 cut(s) 40, 108
Bsp13I TCCGGA 2 cut(s) 196, 328
BspACI CCGC 1 cut(s) 377
BspANI GGCC 2 cut(s) 40, 108
BspCNI CTCAG 1 cut(s) 200
BspEI TCCGGA 2 cut(s) 196, 328
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 322
BspMI ACCTGC 1 cut(s) 19
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 103
BssSI CACGAG 1 cut(s) 314
BssT1I CCWWGG 1 cut(s) 103
Bst2BI CACGAG 1 cut(s) 314
Bst4CI ACNGT 3 cut(s) 217, 296, 304
Bst6I CTCTTC 2 cut(s) 15, 327
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 187
BstF5I GGATG 2 cut(s) 205, 385
BstMAI GTCTC 1 cut(s) 62
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNSI RCATGY 2 cut(s) 86, 148
BstV1I GCAGC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 40, 108
BtgZI GCGATG 1 cut(s) 281
BtrI CACGTC 1 cut(s) 374
BtsCI GGATG 2 cut(s) 205, 385
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 146
CciI TCATGA 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 39
CviAII CATG 3 cut(s) 49, 83, 145
CviJI RGCY 5 cut(s) 40, 95, 108, 158, 313
CviKI_1 RGCY 5 cut(s) 40, 95, 108, 158, 313
DdeI CTNAG 1 cut(s) 187
Eam1104I CTCTTC 2 cut(s) 15, 327
EarI CTCTTC 2 cut(s) 15, 327
Eco130I CCWWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 3 cut(s) 52, 86, 148
FaiI YATR 5 cut(s) 50, 84, 146, 161, 388
FaqI GGGAC 2 cut(s) 89, 305
FatI CATG 3 cut(s) 48, 82, 144
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 1 cut(s) 26
FspBI CTAG 1 cut(s) 36
GluI GCNGC 1 cut(s) 26
HaeIII GGCC 2 cut(s) 40, 108
HapII CCGG 2 cut(s) 197, 329
Hin1II CATG 3 cut(s) 52, 86, 148
HincII GTYRAC 4 cut(s) 11, 58, 226, 307
HindII GTYRAC 4 cut(s) 11, 58, 226, 307
HinfI GANTC 2 cut(s) 59, 77
HpaII CCGG 2 cut(s) 197, 329
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 4 cut(s) 11, 58, 226, 307
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 4 cut(s) 49, 197, 329, 369
Hpy8I GTNNAC 4 cut(s) 11, 58, 226, 307
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 2 cut(s) 46, 159
HpyCH4III ACNGT 3 cut(s) 217, 296, 304
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 4 cut(s) 122, 144, 299, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 1 cut(s) 187
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 3 cut(s) 52, 86, 148
Kpn2I TCCGGA 2 cut(s) 196, 328
LguI GCTCTTC 1 cut(s) 15
LpnPI CCDG 4 cut(s) 14, 210, 342, 382
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 1 cut(s) 258
MaeI CTAG 1 cut(s) 36
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 31
MboII GAAGA 3 cut(s) 32, 163, 344
MluCI AATT 1 cut(s) 180
MlyI GAGTC 2 cut(s) 53, 71
MnlI CCTC 5 cut(s) 169, 196, 223, 232, 346
MroI TCCGGA 2 cut(s) 196, 328
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 1 cut(s) 234
MspI CCGG 2 cut(s) 197, 329
MwoI GCNNNNNNNGC 1 cut(s) 383
NlaIII CATG 3 cut(s) 52, 86, 148
NlaIV GGNNCC 1 cut(s) 322
NspI RCATGY 2 cut(s) 86, 148
PaeI GCATGC 1 cut(s) 148
PagI TCATGA 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 167
PkrI GCNGC 1 cut(s) 27
PleI GAGTC 2 cut(s) 53, 71
PpsI GAGTC 2 cut(s) 53, 71
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 39
SalI GTCGAC 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 26
Sau96I GGNCC 1 cut(s) 39
SchI GAGTC 2 cut(s) 53, 71
SetI ASST 8 cut(s) 10, 33, 57, 234, 243, 278, 315, 376
SfaNI GCATC 1 cut(s) 258
SphI GCATGC 1 cut(s) 148
Sse9I AATT 1 cut(s) 180
SsiI CCGC 1 cut(s) 377
SspMI CTAG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 3 cut(s) 217, 296, 304
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 174, 225
TasI AATT 1 cut(s) 180
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 65, 176
TspRI CASTG 1 cut(s) 136
XceI RCATGY 2 cut(s) 86, 148
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.