pycom10g26320

Thioredoxin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014533
Physical Location & Seq
Forward (+)
27654073 .. 27655383
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26320.1

Sequence Viewer

Length: 687 bp
ATGAGGGTAAAGGGGAATAAATCATCATACCATAGTTTGCACTATATAACGGTGGTGGGTCCAAAAACATTCATATCTATCCTAATCTCTTTTCTCCCCTTTTTGATAAAACTAAACTATCTAGCCCGTTGTTTTCAAACCGTGACAAAAAATAAGAATAAAGTGGTATTAGCAGCATCAGAGTTCCAGAGTTATACACACAGTCACGGATATCCGAATCAAACTACAGAAGGCCACCTAACTGAGCCAACCGTGCCAAGCAAGAGAAGCAACAGAAAGCAGAGAAGGAAGGGGAAGATGGCAGGTCAACTACAAGAAGAGCAGCAGGTAACTAGGCCCAAAGTCATGAAGGTTGACTCTGTGGAGACTTGGGACTCGTATGTTTCCCAAGCCACCAACCAAGGCTGTCCTATTATTACACATTTCACTGCTTCGTGGTGCATGCCTTCGGTGGCTATGAACTCATTCTTCGAGGAAATTGCCTCAGATTATCCGGATGTTCTGTTTCTCACCGTTGATGTCGACGAGGTTAAGGAGGTAGCGACCCGACTGGAGATAAAGGCGATGCCAACGTTTTCGCTGATAAGGGGCGGTGCAACGGTTGACAAGCTCGTGGGTGCCAATCCGGAAGAGATAAGGAAAAGGATTGAGGGTTTCCTTCAGTCCAAACGTGTGGATGTTGCGTAG

Protein Analysis

229

Amino Acids

25.75

Weight (kDa)

9.38

Isoelectric Point (pI)

52.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 119 - 216 2.5e-18 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11530
fragaria_vesca FvH4_3g02911
malus_domestica MD05G1336400.v1.1 MD10G1311900.v1.1
prunus_persica Prupe.4G029100_v2.0.a1
pyrus_communis pycom05g30790 pycom10g26320
rosa_chinensis RchiOBHm_Chr5g0004481
rosa_laevigata RLG00000031232
rosa_multiflora Rmu_sc0000485.1_g000001
rosa_roxburghii Rroxscaffold_1G00071040
rosa_rugosa Rorug04G0412100
rosa_samantha Rh5AG039700 Rh5BG038700 Rh5CG042800 Rh5DG038500
rosa_wichuraiana Rw5G003790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 293, 316
AccB1I GGYRCC 1 cut(s) 617
AccI GTMKAC 1 cut(s) 522
AccIII TCCGGA 2 cut(s) 493, 625
AciI CCGC 1 cut(s) 591
AclI AACGTT 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 644
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 1 cut(s) 137
AluBI AGCT 1 cut(s) 610
AluI AGCT 1 cut(s) 610
Alw26I GTCTC 1 cut(s) 359
Aor13HI TCCGGA 2 cut(s) 493, 625
AoxI GGCC 2 cut(s) 232, 335
ApeKI GCWGC 2 cut(s) 173, 322
Asp700I GAANNNNTTC 1 cut(s) 464
AspS9I GGNCC 2 cut(s) 59, 336
AsuHPI GGTGA 1 cut(s) 502
AvaII GGWCC 1 cut(s) 59
BanI GGYRCC 1 cut(s) 617
BauI CACGAG 1 cut(s) 611
BbvI GCAGC 2 cut(s) 185, 334
BccI CCATC 1 cut(s) 292
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BcoDI GTCTC 1 cut(s) 359
BfaI CTAG 2 cut(s) 122, 333
BfmI CTRYAG 1 cut(s) 225
BfuAI ACCTGC 2 cut(s) 293, 316
BisI GCNGC 2 cut(s) 174, 323
BlsI GCNGC 2 cut(s) 175, 324
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 59, 336
BmiI GGNNCC 2 cut(s) 60, 619
BmsI GCATC 2 cut(s) 185, 555
BpmI CTGGAG 1 cut(s) 572
BsaJI CCNNGG 1 cut(s) 400
BsaWI WCCGGW 2 cut(s) 493, 625
Bse1I ACTGG 1 cut(s) 555
BseAI TCCGGA 2 cut(s) 493, 625
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 2 cut(s) 502, 682
BseMII CTCAG 2 cut(s) 234, 498
BseNI ACTGG 1 cut(s) 555
BseXI GCAGC 2 cut(s) 185, 334
BshFI GGCC 2 cut(s) 234, 337
BshNI GGYRCC 1 cut(s) 617
BsiSI CCGG 2 cut(s) 494, 626
BslFI GGGAC 1 cut(s) 386
BsmAI GTCTC 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 386
BsnI GGCC 2 cut(s) 234, 337
Bsp13I TCCGGA 2 cut(s) 493, 625
BspACI CCGC 1 cut(s) 591
BspANI GGCC 2 cut(s) 234, 337
BspCNI CTCAG 2 cut(s) 235, 497
BspEI TCCGGA 2 cut(s) 493, 625
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 60, 619
BspMI ACCTGC 2 cut(s) 293, 316
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 617
BsrI ACTGG 1 cut(s) 555
BssECI CCNNGG 1 cut(s) 400
BssSI CACGAG 1 cut(s) 611
BssT1I CCWWGG 1 cut(s) 400
Bst2BI CACGAG 1 cut(s) 611
Bst4CI ACNGT 6 cut(s) 52, 142, 203, 253, 514, 601
Bst6I CTCTTC 2 cut(s) 312, 624
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 243, 484
BstF5I GGATG 2 cut(s) 502, 682
BstMAI GTCTC 1 cut(s) 359
BstMWI GCNNNNNNNGC 2 cut(s) 253, 267
BstNSI RCATGY 1 cut(s) 445
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 2 cut(s) 185, 334
BstXI CCANNNNNNTGG 1 cut(s) 673
BsuRI GGCC 2 cut(s) 234, 337
BtgZI GCGATG 1 cut(s) 578
BtsCI GGATG 2 cut(s) 502, 682
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 2 cut(s) 293, 316
Cac8I GCNNGC 1 cut(s) 443
CciI TCATGA 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 59, 336
CviAII CATG 2 cut(s) 346, 442
CviJI RGCY 8 cut(s) 125, 234, 247, 337, 392, 405, 455, 610
CviKI_1 RGCY 8 cut(s) 125, 234, 247, 337, 392, 405, 455, 610
DdeI CTNAG 2 cut(s) 243, 484
Eam1104I CTCTTC 2 cut(s) 312, 624
EarI CTCTTC 2 cut(s) 312, 624
Eco130I CCWWGG 1 cut(s) 400
Eco32I GATATC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 644
EcoRV GATATC 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 2 cut(s) 349, 445
FaqI GGGAC 1 cut(s) 386
FatI CATG 2 cut(s) 345, 441
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 174, 323
FokI GGATG 1 cut(s) 509
Fsp4HI GCNGC 2 cut(s) 174, 323
FspBI CTAG 2 cut(s) 122, 333
GluI GCNGC 2 cut(s) 174, 323
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 2 cut(s) 234, 337
HapII CCGG 2 cut(s) 494, 626
Hin1II CATG 2 cut(s) 349, 445
HincII GTYRAC 4 cut(s) 308, 355, 523, 604
HindII GTYRAC 4 cut(s) 308, 355, 523, 604
HinfI GANTC 3 cut(s) 217, 356, 374
HpaII CCGG 2 cut(s) 494, 626
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 4 cut(s) 308, 355, 523, 604
Hpy188I TCNGA 3 cut(s) 181, 216, 487
Hpy188III TCNNGA 4 cut(s) 187, 346, 494, 626
Hpy8I GTNNAC 4 cut(s) 308, 355, 523, 604
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 6 cut(s) 224, 279, 283, 343, 456, 668
HpyCH4III ACNGT 6 cut(s) 52, 142, 203, 253, 514, 601
HpyCH4IV ACGT 2 cut(s) 572, 670
HpyCH4V TGCA 3 cut(s) 40, 441, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 267
HpyF3I CTNAG 2 cut(s) 243, 484
HpySE526I ACGT 2 cut(s) 572, 670
Hsp92II CATG 2 cut(s) 349, 445
Kpn2I TCCGGA 2 cut(s) 493, 625
LguI GCTCTTC 1 cut(s) 312
LpnPI CCDG 6 cut(s) 200, 288, 311, 507, 536, 639
Lsp1109I GCAGC 2 cut(s) 185, 334
LweI GCATC 2 cut(s) 185, 555
MaeI CTAG 2 cut(s) 122, 333
MaeII ACGT 2 cut(s) 572, 670
MaeIII GTNAC 3 cut(s) 142, 203, 328
MboII GAAGA 4 cut(s) 307, 329, 460, 641
MluCI AATT 1 cut(s) 477
MlyI GAGTC 2 cut(s) 350, 368
MnlI CCTC 5 cut(s) 466, 493, 520, 529, 643
MroI TCCGGA 2 cut(s) 493, 625
MroXI GAANNNNTTC 1 cut(s) 464
MseI TTAA 1 cut(s) 531
MspI CCGG 2 cut(s) 494, 626
MwoI GCNNNNNNNGC 2 cut(s) 253, 267
NlaIII CATG 2 cut(s) 349, 445
NlaIV GGNNCC 2 cut(s) 60, 619
NmuCI GTSAC 2 cut(s) 142, 203
NspI RCATGY 1 cut(s) 445
PaeI GCATGC 1 cut(s) 445
PagI TCATGA 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 217
PkrI GCNGC 2 cut(s) 175, 324
PleI GAGTC 2 cut(s) 350, 368
PpsI GAGTC 2 cut(s) 350, 368
Psp1406I AACGTT 1 cut(s) 572
PspN4I GGNNCC 2 cut(s) 60, 619
PspPI GGNCC 2 cut(s) 59, 336
SalI GTCGAC 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 2 cut(s) 174, 323
Sau96I GGNCC 2 cut(s) 59, 336
SchI GAGTC 2 cut(s) 350, 368
SetI ASST 9 cut(s) 240, 307, 330, 354, 531, 540, 575, 612, 673
SfaNI GCATC 2 cut(s) 185, 555
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 1 cut(s) 59
SphI GCATGC 1 cut(s) 445
Sse9I AATT 1 cut(s) 477
SsiI CCGC 1 cut(s) 591
SspMI CTAG 2 cut(s) 122, 333
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 6 cut(s) 52, 142, 203, 253, 514, 601
TaiI ACGT 2 cut(s) 575, 673
TaqI TCGA 2 cut(s) 471, 522
TasI AATT 1 cut(s) 477
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 2 cut(s) 142, 203
TseI GCWGC 2 cut(s) 173, 322
Tsp45I GTSAC 2 cut(s) 142, 203
TspDTI ATGAA 3 cut(s) 61, 362, 473
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 59
XceI RCATGY 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 2 cut(s) 122, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.