MD10G1332100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012145
Physical Location & Seq
Reverse (-)
40960840 .. 40963426
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1332100.v1.1.491

Sequence Viewer

Length: 765 bp
ATGACTTTGGTTATTTGCCCTACCGGGGCCATTGTTCACAAAAATTTGAAGAAAACAGCATCTTCTTGTAATGTTTTTGGCAATTTTTATCCACCAAGTTGTGATCATCGACAACCTAGTATTCATGAGAGCATCTTTCCAAAATCAAAGAAGAGAGACTTCTTGTTAGTGCCAGTCAGTTCTGCTAGAAACTCAGGAGGATTACCAGGGGATCATCAGGGAGCCGAGGATCCACGAGCTCTGGAAACAGTCCTCAAACTCTACACCGCTATCAAGAACAAAAACATTCGCGAGTTGTCTGAGATTATAGGAGATGAATGCCAATGTGTATGCAATTTATTCTCAGTCATCCAACCCCTCCGAGGAAAGAAGCAAGTGATGGATTTTTTCTCATATCTAATCCGAAGCTTGGGAAGTAACATCGAATTTGTTATAACGCCGACGTTGCACCATGGAATGAACGTCGGTGTTCAGTGGAGCTTAGAGTGGAACAAAACGCATGTGCCTCTGGGAAAGGGCTTTAGCTTCCATATATGCCAAATCTATAAAGGGAAGGTCATCATAAGAAATGTGGAGATGTTTATGGAACCACTCCTCCATATTGAACCCCTCAGACTGAAAATAATGGGAATTATGGCGGGTTTAATGGAAAAGATGGGTTCCAATTCAATGTTCAAGGGTAAAGCAAACGGAATTCTGTGGGTTTTACTCTTTCTGTTCATCAAGGCTGCCTTTTTAATCTTTTTCAAACTTATCTTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.62

Weight (kDa)

9.4

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22370 AT4G22370 AT4G22370
fragaria_vesca FvH4_3g00940 FvH4_3g00940
malus_domestica MD10G1332100.v1.1
prunus_persica Prupe.4G010000_v2.0.a1 Prupe.4G010000_v2.0.a1
pyrus_communis pycom10g28150
rosa_chinensis RchiOBHm_Chr5g0001461
rosa_laevigata RLG00000030930
rosa_multiflora Rmu_sc0003749.1_g000012
rosa_roxburghii Rroxscaffold_1G00074930
rosa_rugosa Rorug04G0390400
rosa_samantha Rh5AG011300 Rh5BG013800 Rh5CG012300 Rh5DG011500
rosa_wichuraiana Rw5G001070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AccII CGCG 1 cut(s) 291
AciI CCGC 2 cut(s) 267, 638
AclWI GGATC 3 cut(s) 219, 224, 237
AcsI RAATTY 3 cut(s) 43, 425, 693
AfiI CCNNNNNNNGG 3 cut(s) 25, 362, 409
AgsI TTSAA 5 cut(s) 49, 605, 669, 676, 748
AjnI CCWGG 1 cut(s) 205
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AloI GAACNNNNNNTCC 2 cut(s) 579, 611
AluBI AGCT 4 cut(s) 239, 408, 480, 525
AluI AGCT 4 cut(s) 239, 408, 480, 525
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 3 cut(s) 219, 224, 237
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 3 cut(s) 43, 425, 693
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 1 cut(s) 25
BamHI GGATCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 241
BauI CACGAG 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 1 cut(s) 715
BccI CCATC 2 cut(s) 373, 649
BciT130I CCWGG 1 cut(s) 207
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 117, 186
BisI GCNGC 1 cut(s) 729
BlsI GCNGC 1 cut(s) 730
Bme1390I CCNGG 2 cut(s) 25, 207
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 5 cut(s) 28, 223, 231, 588, 661
BmrFI CCNGG 2 cut(s) 25, 207
BmsI GCATC 2 cut(s) 68, 141
BpuMI CCSGG 1 cut(s) 25
BsaJI CCNNGG 5 cut(s) 24, 206, 225, 361, 451
BsaXI ACNNNNNCTCC 2 cut(s) 579, 609
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 362, 409
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 5 cut(s) 24, 206, 225, 361, 451
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 3 cut(s) 25, 362, 409
BseMII CTCAG 4 cut(s) 207, 291, 357, 625
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 1 cut(s) 715
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 3 cut(s) 25, 362, 409
BsmAI GTCTC 1 cut(s) 150
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 241
Bsp143I GATC 3 cut(s) 103, 211, 229
Bsp19I CCATGG 1 cut(s) 451
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 2 cut(s) 267, 638
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 4 cut(s) 206, 292, 356, 624
BspFNI CGCG 1 cut(s) 291
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 5 cut(s) 28, 223, 231, 588, 661
BspPI GGATC 3 cut(s) 219, 224, 237
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 5 cut(s) 24, 206, 225, 361, 451
BssMI GATC 3 cut(s) 103, 211, 229
BssSI CACGAG 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 451
Bst2BI CACGAG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 146
BstDEI CTNAG 5 cut(s) 193, 300, 343, 481, 611
BstDSI CCRYGG 1 cut(s) 451
BstF5I GGATG 1 cut(s) 348
BstFNI CGCG 1 cut(s) 291
BstKTI GATC 3 cut(s) 106, 214, 232
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 3 cut(s) 103, 211, 229
BstMWI GCNNNNNNNGC 1 cut(s) 445
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 503
BstSCI CCNGG 2 cut(s) 23, 205
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 715
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 451
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 479
BtuMI TCGCGA 1 cut(s) 291
CciI TCATGA 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 27
CviAII CATG 3 cut(s) 125, 452, 500
CviJI RGCY 8 cut(s) 29, 224, 239, 408, 480, 519, 525, 728
CviKI_1 RGCY 8 cut(s) 29, 224, 239, 408, 480, 519, 525, 728
DdeI CTNAG 5 cut(s) 193, 300, 343, 481, 611
DpnI GATC 3 cut(s) 105, 213, 231
DpnII GATC 3 cut(s) 103, 211, 229
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 239
EcoICRI GAGCTC 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 3 cut(s) 128, 455, 503
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 716, 748
FatI CATG 3 cut(s) 124, 451, 499
FauI CCCGC 1 cut(s) 631
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 1 cut(s) 729
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 1 cut(s) 729
FspBI CTAG 2 cut(s) 117, 186
GluI GCNGC 1 cut(s) 729
HaeIII GGCC 1 cut(s) 29
HapII CCGG 1 cut(s) 24
Hin1II CATG 3 cut(s) 128, 455, 503
HindIII AAGCTT 1 cut(s) 406
HpaII CCGG 1 cut(s) 24
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 4 cut(s) 301, 362, 404, 614
Hpy188III TCNNGA 5 cut(s) 125, 195, 242, 274, 290
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 2 cut(s) 445, 467
HpyAV CCTTC 1 cut(s) 547
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 2 cut(s) 443, 462
HpyCH4V TGCA 2 cut(s) 333, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 445
HpyF3I CTNAG 5 cut(s) 193, 300, 343, 481, 611
HpySE526I ACGT 2 cut(s) 443, 462
Hsp92II CATG 3 cut(s) 128, 455, 503
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 3 cut(s) 103, 211, 229
LmnI GCTCC 2 cut(s) 221, 477
LpnPI CCDG 8 cut(s) 37, 180, 186, 192, 203, 219, 227, 494
Lsp1109I GCAGC 1 cut(s) 715
LweI GCATC 2 cut(s) 68, 141
MaeI CTAG 2 cut(s) 117, 186
MaeII ACGT 2 cut(s) 443, 462
MaeIII GTNAC 1 cut(s) 416
MalI GATC 3 cut(s) 105, 213, 231
MboI GATC 3 cut(s) 103, 211, 229
MboII GAAGA 3 cut(s) 54, 61, 163
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 7 cut(s) 43, 82, 334, 425, 630, 664, 693
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 8 cut(s) 191, 220, 263, 356, 368, 516, 605, 620
MseI TTAA 3 cut(s) 644, 737, 763
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 2 cut(s) 25, 207
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 445
NciI CCSGG 1 cut(s) 25
NcoI CCATGG 1 cut(s) 451
NdeII GATC 3 cut(s) 103, 211, 229
NlaIII CATG 3 cut(s) 128, 455, 503
NlaIV GGNNCC 5 cut(s) 28, 223, 231, 588, 661
NmeAIII GCCGAG 1 cut(s) 250
NruI TCGCGA 1 cut(s) 291
NspI RCATGY 1 cut(s) 503
PagI TCATGA 1 cut(s) 124
PctI GAATGC 1 cut(s) 323
PkrI GCNGC 1 cut(s) 730
PsiI TTATAA 1 cut(s) 434
Psp124BI GAGCTC 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 5 cut(s) 28, 223, 231, 588, 661
PspPI GGNCC 1 cut(s) 27
PsuI RGATCY 1 cut(s) 229
RruI TCGCGA 1 cut(s) 291
SacI GAGCTC 1 cut(s) 241
SaqAI TTAA 3 cut(s) 644, 737, 763
SatI GCNGC 1 cut(s) 729
Sau3AI GATC 3 cut(s) 103, 211, 229
Sau96I GGNCC 1 cut(s) 27
ScrFI CCNGG 2 cut(s) 25, 207
SduI GDGCHC 1 cut(s) 241
SetI ASST 8 cut(s) 118, 241, 410, 446, 465, 482, 527, 558
SfaNI GCATC 2 cut(s) 68, 141
Sse9I AATT 7 cut(s) 43, 82, 334, 425, 630, 664, 693
SsiI CCGC 2 cut(s) 267, 638
SspMI CTAG 2 cut(s) 117, 186
SstI GAGCTC 1 cut(s) 241
StyD4I CCNGG 2 cut(s) 23, 205
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 2 cut(s) 446, 465
TaqI TCGA 2 cut(s) 109, 423
TasI AATT 7 cut(s) 43, 82, 334, 425, 630, 664, 693
Tru1I TTAA 3 cut(s) 644, 737, 763
Tru9I TTAA 3 cut(s) 644, 737, 763
TscAI CASTG 1 cut(s) 479
TseI GCWGC 1 cut(s) 728
TspDTI ATGAA 4 cut(s) 113, 330, 473, 709
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 1 cut(s) 479
XapI RAATTY 3 cut(s) 43, 425, 693
XceI RCATGY 1 cut(s) 503
XspI CTAG 2 cut(s) 117, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.