MD10G1336400.v1.1

Belongs to the NARF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013504
Physical Location & Seq
Forward (+)
41285178 .. 41288271
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1336400.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGTTTAAGCTTTTTAGTAATTTTTTTGGTGTGAAGGTTTCAACTTCTAGCAAGCAAGTGAAAACAGAACCAGTTAAAATTTCACTCAAAGATTGCTTGGCATGCAGTGGTTGTGTAACATCAGCCGAGACAGTTATGCTGGAGAAGCAAAGTTTGGATGAGTTTCTTCTTAATCTTGACAAAGGAAAAGCTGTCACTGTCTCCCTATCTCCCCAATCAAGAGCGTCCCTTGCCGTTTATTTTGGAATTTCACCACTTCAGGTCTCTAGGAAACTCACAACTTTTTTTAAGTCCTTGGGTGTAAAGGCTGTATTTGACACAAGTTGCAGTCGAGATTTAACACTTGTTGAATATTGTAACGAGTTCATCACAAGGTACAAACAAAGCCGGTCATCTGATGGTGAAAGGTGTCAATCTTTGTTGCCCATGATTTCATCAGCATGTCCAGGTTGGATATGCTATGCTGAAAAACAACTTGGATCCTATGTTCTGCCTTATATTTCATCAAGGAAGAGTCCCCAGCAAACAATTGGAGTTATTGTTAAAACTCATGTATGCCAAAAAATAGGTCTAAGGACAGCTGATGTTTACCATGTAACTGTGATGCCTTGTTATGATAAAATGCTTGAGGCTTCACGTGATGACTTCGTTTTTGAAGTTGATCAATCTGAAAATAATGAGAATGAAGGTCTAAGGGTTACAGAGGTAGACTCGGTGTTGACATCCGGGGAAGTTTTAGAACTGATACAGTTGAAAGGAGTAGATTTCAAAGGCTTAGAAGAGTCCCCTCTAGATAACATGCTGACAAATTTTAATGAAGGGAGTCTTTTTGGTGTCCATGGAAGCTTAGGGGGTTATGAGGAGACAATATTCAGCTATGCTGCTGAAGTGCTCTTTGGAAGAGAAGTCAAGGGCCCTATTGATTTCAATTCAGTTAAAAATTCAGACTTCCAGGAACTGACTTTGGAAGCAAGTCAAAGTATTGATGGCCTCTCGTTTAGGTGGATGGGAAGACTATTGAAATTTGCACTGTGTTATGGCTTCCGTAACCTGCAAAGCGTTGTCAGGAAAAAAAAAATTGGAAAATGCGATTATCATTTTCTAGAGATCATGGCATGCCCTTCTGGTGGTTGCTTAAATGGTGAAGGACAGATTAAGCCACAGCCTGAACAATCCGGAAAGGAGTTGATTCAGTTATTACAAGCAGCTTATATGGAAAACTTAAGGGTCTTGGTAGCTGATCCTTTTGGAAATCCTTTGGTGCAAAACTTATGCGACGAGTGGCTCGAGCATCCTGGATAG

Protein Analysis

434

Amino Acids

48.17

Weight (kDa)

5.5

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe_hyd_lg_C PF02906 63 - 381 1e-67 Iron only hydrogenase large subunit, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013504)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16440
fragaria_vesca FvH4_3g00590
malus_domestica MD00G1058500.v1.1 MD10G1336400.v1.1
prunus_persica Prupe.4G007100_v2.0.a1
pyrus_communis pycom05g32570
rosa_chinensis RchiOBHm_Chr5g0000801
rosa_laevigata RLG00000030897
rosa_multiflora Rmu_sc0000255.1_g000009
rosa_roxburghii Rroxscaffold_1G00075430
rosa_rugosa Rorug04G0386700 Rorug04G0386800.1 Rorug04G0386900
rosa_samantha Rh5AG006600 Rh5BG008600 Rh5CG007200 Rh5DG007200
rosa_wichuraiana Rw5G000680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1059
AccI GTMKAC 1 cut(s) 708
AccIII TCCGGA 1 cut(s) 1175
AclWI GGATC 3 cut(s) 474, 487, 1235
AcsI RAATTY 5 cut(s) 78, 246, 808, 940, 1022
AcuI CTGAAG 2 cut(s) 242, 906
AcvI CACGTG 1 cut(s) 638
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 1 cut(s) 1127
AflII CTTAAG 1 cut(s) 1222
AgsI TTSAA 7 cut(s) 42, 350, 656, 754, 769, 928, 1021
AjnI CCWGG 3 cut(s) 445, 951, 1294
AjuI GAANNNNNNNTTGG 6 cut(s) 137, 169, 459, 491, 879, 911
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
AluBI AGCT 7 cut(s) 10, 191, 581, 846, 876, 1208, 1238
AluI AGCT 7 cut(s) 10, 191, 581, 846, 876, 1208, 1238
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 4 cut(s) 122, 205, 268, 857
AlwI GGATC 3 cut(s) 474, 487, 1235
AlwNI CAGNNNCTG 1 cut(s) 958
Ama87I CYCGRG 1 cut(s) 1286
Aor13HI TCCGGA 1 cut(s) 1175
AoxI GGCC 2 cut(s) 913, 988
ApaI GGGCCC 1 cut(s) 917
ApeKI GCWGC 2 cut(s) 881, 1205
ApoI RAATTY 5 cut(s) 78, 246, 808, 940, 1022
ArsI GACNNNNNNTTYG 4 cut(s) 553, 585, 635, 667
AspS9I GGNCC 2 cut(s) 913, 914
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 3 cut(s) 243, 413, 1154
AvaI CYCGRG 1 cut(s) 1286
BaeGI GKGCMC 1 cut(s) 917
BamHI GGATCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 917
BbrPI CACGTG 1 cut(s) 638
BbsI GAAGAC 1 cut(s) 1018
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 2 cut(s) 868, 1217
BccI CCATC 3 cut(s) 392, 980, 1000
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 3 cut(s) 447, 953, 1296
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 4 cut(s) 122, 205, 268, 857
BfaI CTAG 4 cut(s) 48, 267, 791, 1103
BfrI CTTAAG 1 cut(s) 1222
BfuAI ACCTGC 1 cut(s) 1059
BisI GCNGC 2 cut(s) 882, 1206
BlsI GCNGC 2 cut(s) 883, 1207
Bme1390I CCNGG 4 cut(s) 447, 727, 953, 1296
BmeT110I CYCGRG 1 cut(s) 1286
BmgT120I GGNCC 2 cut(s) 913, 914
BmiI GGNNCC 2 cut(s) 481, 915
BmrFI CCNGG 4 cut(s) 447, 727, 953, 1296
BmsI GCATC 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 1018
BplI GAGNNNNNCTC 2 cut(s) 695, 727
BpmI CTGGAG 1 cut(s) 161
Bpu10I CCTNAGC 1 cut(s) 847
BpuEI CTTGAG 1 cut(s) 647
BpuMI CCSGG 1 cut(s) 727
BsaAI YACGTR 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 268
BsaJI CCNNGG 3 cut(s) 294, 726, 838
BsaWI WCCGGW 1 cut(s) 1175
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 1 cut(s) 1127
Bse118I RCCGGY 1 cut(s) 387
Bse1I ACTGG 1 cut(s) 71
BseAI TCCGGA 1 cut(s) 1175
BseBI CCWGG 3 cut(s) 447, 953, 1296
BseDI CCNNGG 3 cut(s) 294, 726, 838
BseGI GGATG 4 cut(s) 163, 722, 1011, 1291
BseLI CCNNNNNNNGG 1 cut(s) 1127
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 1 cut(s) 875
BseSI GKGCMC 1 cut(s) 917
BseXI GCAGC 2 cut(s) 868, 1217
BseYI CCCAGC 1 cut(s) 519
BshFI GGCC 2 cut(s) 915, 990
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 1286
BsiSI CCGG 3 cut(s) 388, 726, 1176
BslFI GGGAC 3 cut(s) 211, 501, 769
BslI CCNNNNNNNGG 1 cut(s) 1127
BsmAI GTCTC 4 cut(s) 122, 205, 268, 857
BsmFI GGGAC 3 cut(s) 211, 501, 769
BsnI GGCC 2 cut(s) 915, 990
Bso31I GGTCTC 1 cut(s) 268
BsoBI CYCGRG 1 cut(s) 1286
Bsp120I GGGCCC 1 cut(s) 913
Bsp1286I GDGCHC 2 cut(s) 894, 917
Bsp13I TCCGGA 1 cut(s) 1175
Bsp143I GATC 4 cut(s) 479, 661, 1107, 1240
Bsp19I CCATGG 1 cut(s) 838
BspANI GGCC 2 cut(s) 915, 990
BspEI TCCGGA 1 cut(s) 1175
BspLI GGNNCC 2 cut(s) 481, 915
BspMI ACCTGC 1 cut(s) 1059
BspPI GGATC 3 cut(s) 474, 487, 1235
BspTI CTTAAG 1 cut(s) 1222
BspTNI GGTCTC 1 cut(s) 268
BsrFI RCCGGY 1 cut(s) 387
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 387
BssECI CCNNGG 3 cut(s) 294, 726, 838
BssMI GATC 4 cut(s) 479, 661, 1107, 1240
BssT1I CCWWGG 2 cut(s) 294, 838
Bst2UI CCWGG 3 cut(s) 447, 953, 1296
Bst4CI ACNGT 5 cut(s) 133, 199, 601, 750, 1032
Bst6I CTCTTC 3 cut(s) 506, 774, 895
BstAFI CTTAAG 1 cut(s) 1222
BstBAI YACGTR 1 cut(s) 638
BstC8I GCNNGC 3 cut(s) 53, 103, 1117
BstDEI CTNAG 4 cut(s) 572, 692, 775, 847
BstDSI CCRYGG 1 cut(s) 838
BstF5I GGATG 4 cut(s) 163, 722, 1011, 1291
BstKTI GATC 4 cut(s) 482, 664, 1110, 1243
BstMAI GTCTC 4 cut(s) 122, 205, 268, 857
BstMBI GATC 4 cut(s) 479, 661, 1107, 1240
BstMWI GCNNNNNNNGC 3 cut(s) 102, 145, 230
BstNI CCWGG 3 cut(s) 447, 953, 1296
BstNSI RCATGY 4 cut(s) 105, 444, 802, 1119
BstSCI CCNGG 4 cut(s) 445, 725, 951, 1294
BstSLI GKGCMC 1 cut(s) 917
BstV1I GCAGC 2 cut(s) 868, 1217
BstV2I GAAGAC 1 cut(s) 1018
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BsuRI GGCC 2 cut(s) 915, 990
BtgI CCRYGG 1 cut(s) 838
BtsCI GGATG 4 cut(s) 163, 722, 1011, 1291
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 3 cut(s) 112, 195, 1028
BveI ACCTGC 1 cut(s) 1059
Cac8I GCNNGC 3 cut(s) 53, 103, 1117
CaiI CAGNNNCTG 1 cut(s) 958
Cfr10I RCCGGY 1 cut(s) 387
Cfr13I GGNCC 2 cut(s) 913, 914
CseI GACGC 1 cut(s) 213
Csp6I GTAC 1 cut(s) 376
CviAII CATG 9 cut(s) 102, 427, 441, 551, 593, 799, 839, 1111, 1116
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 4 cut(s) 572, 692, 775, 847
DpnI GATC 4 cut(s) 481, 663, 1109, 1242
DpnII GATC 4 cut(s) 479, 661, 1107, 1240
Eam1104I CTCTTC 3 cut(s) 506, 774, 895
EarI CTCTTC 3 cut(s) 506, 774, 895
Eco130I CCWWGG 2 cut(s) 294, 838
Eco24I GRGCYC 1 cut(s) 917
Eco31I GGTCTC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 242, 906
Eco72I CACGTG 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 1286
EcoO109I RGGNCCY 2 cut(s) 913, 914
EcoRII CCWGG 3 cut(s) 445, 951, 1294
EcoT14I CCWWGG 2 cut(s) 294, 838
EcoT38I GRGCYC 1 cut(s) 917
ErhI CCWWGG 2 cut(s) 294, 838
FaeI CATG 9 cut(s) 105, 430, 444, 554, 596, 802, 842, 1114, 1119
FalI AAGNNNNNCTT 2 cut(s) 810, 842
FaqI GGGAC 3 cut(s) 211, 501, 769
FatI CATG 9 cut(s) 101, 426, 440, 550, 592, 798, 838, 1110, 1115
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 2 cut(s) 882, 1206
FokI GGATG 4 cut(s) 170, 709, 1018, 1278
FriOI GRGCYC 1 cut(s) 917
Fsp4HI GCNGC 2 cut(s) 882, 1206
FspBI CTAG 4 cut(s) 48, 267, 791, 1103
GluI GCNGC 2 cut(s) 882, 1206
GsaI CCCAGC 1 cut(s) 523
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 2 cut(s) 915, 990
HapII CCGG 3 cut(s) 388, 726, 1176
HgaI GACGC 1 cut(s) 213
Hin1II CATG 9 cut(s) 105, 430, 444, 554, 596, 802, 842, 1114, 1119
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HindIII AAGCTT 2 cut(s) 8, 844
HinfI GANTC 5 cut(s) 514, 710, 782, 823, 1189
HpaII CCGG 3 cut(s) 388, 726, 1176
HphI GGTGA 3 cut(s) 243, 413, 1154
Hpy166II GTNNAC 3 cut(s) 589, 709, 720
Hpy188I TCNGA 3 cut(s) 397, 670, 946
Hpy188III TCNNGA 7 cut(s) 176, 219, 332, 791, 1066, 1103, 1176
Hpy8I GTNNAC 3 cut(s) 589, 709, 720
Hpy99I CGWCG 1 cut(s) 1280
HpyAV CCTTC 5 cut(s) 28, 680, 812, 1131, 1139
HpyCH4III ACNGT 5 cut(s) 133, 199, 601, 750, 1032
HpyCH4IV ACGT 1 cut(s) 637
HpyCH4V TGCA 5 cut(s) 105, 327, 1028, 1054, 1264
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 145, 230
HpyF3I CTNAG 4 cut(s) 572, 692, 775, 847
HpySE526I ACGT 1 cut(s) 637
Hsp92II CATG 9 cut(s) 105, 430, 444, 554, 596, 802, 842, 1114, 1119
Kpn2I TCCGGA 1 cut(s) 1175
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 4 cut(s) 479, 661, 1107, 1240
Lsp1109I GCAGC 2 cut(s) 868, 1217
LweI GCATC 1 cut(s) 594
MaeI CTAG 4 cut(s) 48, 267, 791, 1103
MaeII ACGT 1 cut(s) 637
MaeIII GTNAC 6 cut(s) 115, 193, 356, 595, 697, 1046
MalI GATC 4 cut(s) 481, 663, 1109, 1242
MboI GATC 4 cut(s) 479, 661, 1107, 1240
MboII GAAGA 5 cut(s) 158, 523, 791, 912, 1023
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 2 cut(s) 894, 917
MluCI AATT 9 cut(s) 19, 78, 246, 528, 808, 928, 940, 1022, 1077
MlyI GAGTC 4 cut(s) 523, 704, 791, 832
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 5 cut(s) 622, 697, 798, 853, 1001
MroI TCCGGA 1 cut(s) 1175
MslI CAYNNNNRTG 1 cut(s) 439
MspA1I CMGCKG 1 cut(s) 581
MspCI CTTAAG 1 cut(s) 1222
MspI CCGG 3 cut(s) 388, 726, 1176
MspR9I CCNGG 4 cut(s) 447, 727, 953, 1296
MunI CAATTG 1 cut(s) 528
MvaI CCWGG 3 cut(s) 447, 953, 1296
MwoI GCNNNNNNNGC 3 cut(s) 102, 145, 230
NciI CCSGG 1 cut(s) 727
NcoI CCATGG 1 cut(s) 838
NdeII GATC 4 cut(s) 479, 661, 1107, 1240
NlaIII CATG 9 cut(s) 105, 430, 444, 554, 596, 802, 842, 1114, 1119
NlaIV GGNNCC 2 cut(s) 481, 915
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 4 cut(s) 105, 444, 802, 1119
PaeI GCATGC 2 cut(s) 105, 1119
PaeR7I CTCGAG 1 cut(s) 1286
PcsI WCGNNNNNNNCGW 1 cut(s) 1284
PfeI GAWTC 1 cut(s) 1189
PfoI TCCNGGA 2 cut(s) 951, 1294
PkrI GCNGC 2 cut(s) 883, 1207
PleI GAGTC 4 cut(s) 522, 704, 790, 831
PmaCI CACGTG 1 cut(s) 638
PmlI CACGTG 1 cut(s) 638
PpsI GAGTC 4 cut(s) 522, 704, 790, 831
Ppu21I YACGTR 1 cut(s) 638
Psp6I CCWGG 3 cut(s) 445, 951, 1294
PspCI CACGTG 1 cut(s) 638
PspFI CCCAGC 1 cut(s) 519
PspGI CCWGG 3 cut(s) 445, 951, 1294
PspN4I GGNNCC 2 cut(s) 481, 915
PspOMI GGGCCC 1 cut(s) 913
PspPI GGNCC 2 cut(s) 913, 914
PspXI VCTCGAGB 1 cut(s) 1286
PstNI CAGNNNCTG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 439
SatI GCNGC 2 cut(s) 882, 1206
Sau3AI GATC 4 cut(s) 479, 661, 1107, 1240
Sau96I GGNCC 2 cut(s) 913, 914
SchI GAGTC 4 cut(s) 523, 704, 791, 832
ScrFI CCNGG 4 cut(s) 447, 727, 953, 1296
SduI GDGCHC 2 cut(s) 894, 917
SfaNI GCATC 1 cut(s) 594
Sfr274I CTCGAG 1 cut(s) 1286
SlaI CTCGAG 1 cut(s) 1286
SmiMI CAYNNNNRTG 1 cut(s) 439
SmlI CTYRAG 3 cut(s) 626, 1222, 1286
SmoI CTYRAG 3 cut(s) 626, 1222, 1286
SphI GCATGC 2 cut(s) 105, 1119
Sse9I AATT 9 cut(s) 19, 78, 246, 528, 808, 928, 940, 1022, 1077
SspI AATATT 2 cut(s) 353, 870
SspMI CTAG 4 cut(s) 48, 267, 791, 1103
StyD4I CCNGG 4 cut(s) 445, 725, 951, 1294
StyI CCWWGG 2 cut(s) 294, 838
TaaI ACNGT 5 cut(s) 133, 199, 601, 750, 1032
TaiI ACGT 1 cut(s) 640
TaqI TCGA 2 cut(s) 331, 1287
TasI AATT 9 cut(s) 19, 78, 246, 528, 808, 928, 940, 1022, 1077
TfiI GAWTC 1 cut(s) 1189
TscAI CASTG 3 cut(s) 112, 202, 1035
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 2 cut(s) 881, 1205
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 5 cut(s) 355, 423, 492, 699, 831
TspGWI ACGGA 1 cut(s) 1034
TspRI CASTG 3 cut(s) 112, 202, 1035
Vha464I CTTAAG 1 cut(s) 1222
XapI RAATTY 5 cut(s) 78, 246, 808, 940, 1022
XbaI TCTAGA 2 cut(s) 790, 1102
XceI RCATGY 4 cut(s) 105, 444, 802, 1119
XcmI CCANNNNNNNNNTGG 1 cut(s) 527
XhoI CTCGAG 1 cut(s) 1286
XmiI GTMKAC 1 cut(s) 708
XspI CTAG 4 cut(s) 48, 267, 791, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.