Rh5BG008600

Belongs to the NARF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013504
Physical Location & Seq
Forward (+)
563185 .. 569245
6061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG008600.1

Sequence Viewer

Length: 1101 bp
ATGTCAGAGAAGTTCTCCGCGACGCTTCGAATCGGAGACCTTAACGACTACATAGCTCCCTCTCAGGCTTGCATAGTTTCTCTCAAATCCACCCCTACAAAGCCCTCTGACAAGTCTCAGGTTGCAACTTCCAGCAAGCAATTGAAAACAGAACCGGTTAAAATTTCACTCAAAGATTGCTTAGCATGCAGTGGGTGCATAACATCGGCTGAGACGGTTATGCTGGAGAAGCAAAGTTTGGATGAGTTTCTTTTGAATATTGATAAAGGAAAAGCTGTCATTGTCTCCCTATCTCCCCAATCAAGAGCTTCACTTGCAGTTCATTTTGGGATTTCGCAACTTCAGGTCTTTAGGAAATTGACAACTCTTTTTAAGTCCCTGGGCGTAAAGGCTATATTTGACACAAGTTGCAGTAGAGATCTAAGTCTTATTGAATCTTGTAATGAATTCATGACAAGGTACAACCAGTCAACTGATGATGAAAGCCGTAAATCTTTGTTGCCCATGATTTCATCAGCATGTCCAGGCTGGATATGTTATGCTGAAAAAACACTTGGATCCTATGTACTGCCTTATATTTCTTCAGTGAAGAGTCCCCAGCAAACAATTGGAGTTATTGTTAAAAAGCATATATGCCAAAAAATGGGTCTTAGGCCAGATGATGTTTACCATGTAACAGTGATGCCTTGTTATGATAAGAAGCTTGAGGCGTCACGCGATGACTTCGTATTTGAAGTTGATGGAGCCAAGAGTAATGAGGATGGAAGTCTTAGCGTTTCAGAGGTAGATTCAGTGTTGACAACTGGGGAAGTTTTAGATCTGATACAGTTAAAAGGAGTTGATTTTGAGGGCTTAGAAGAGTCTCCACTAGATAGCATGTTGACAAACTTTAGTGACGGACATCTTTACGGAGTCCGTGGAAGCTCAGGGGGTTATGCAGAAACAATTTTCCGATATGCTGCTAAAGTAATCTTTGGAAGAGAAATTGAAGGCCCTGTGGACTTCAGAACTATTAAAAATTCAGACTTCCAGGAAGTGACTTTAGAGGTAAGTGAAGTTCTTAAGGATTTGAGTTTGACCACATATTTGCTAGGGTTATAG

Protein Analysis

366

Amino Acids

40.19

Weight (kDa)

5.16

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe_hyd_lg_C PF02906 91 - 350 5.9e-59 Iron only hydrogenase large subunit, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013504)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16440
fragaria_vesca FvH4_3g00590
malus_domestica MD00G1058500.v1.1 MD10G1336400.v1.1
prunus_persica Prupe.4G007100_v2.0.a1
pyrus_communis pycom05g32570
rosa_chinensis RchiOBHm_Chr5g0000801
rosa_laevigata RLG00000030897
rosa_multiflora Rmu_sc0000255.1_g000009
rosa_roxburghii Rroxscaffold_1G00075430
rosa_rugosa Rorug04G0386700 Rorug04G0386800.1 Rorug04G0386900
rosa_samantha Rh5AG006600 Rh5BG008600 Rh5CG007200 Rh5DG007200
rosa_wichuraiana Rw5G000680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 643
AccII CGCG 2 cut(s) 20, 717
AciI CCGC 1 cut(s) 18
AclWI GGATC 2 cut(s) 552, 565
AcsI RAATTY 3 cut(s) 162, 446, 1018
AcuI CTGAAG 3 cut(s) 326, 567, 988
AcyI GRCGYC 1 cut(s) 710
AfaI GTAC 2 cut(s) 461, 567
AfiI CCNNNNNNNGG 1 cut(s) 643
AflII CTTAAG 1 cut(s) 1061
AgeI ACCGGT 1 cut(s) 154
AgsI TTSAA 5 cut(s) 145, 256, 434, 734, 989
AjnI CCWGG 3 cut(s) 378, 523, 1029
AjuI GAANNNNNNNTTGG 4 cut(s) 221, 253, 537, 569
AluBI AGCT 5 cut(s) 56, 275, 308, 703, 924
AluI AGCT 5 cut(s) 56, 275, 308, 703, 924
Alw26I GTCTC 5 cut(s) 30, 120, 206, 289, 867
AlwI GGATC 2 cut(s) 552, 565
AoxI GGCC 2 cut(s) 653, 991
ApeKI GCWGC 1 cut(s) 959
ApoI RAATTY 3 cut(s) 162, 446, 1018
ArsI GACNNNNNNTTYG 6 cut(s) 631, 663, 713, 745, 1069, 1101
AsiGI ACCGGT 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 992
AsuII TTCGAA 1 cut(s) 28
BamHI GGATCC 1 cut(s) 557
BbvI GCAGC 1 cut(s) 946
BccI CCATC 2 cut(s) 734, 755
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 3 cut(s) 380, 525, 1031
BcoDI GTCTC 5 cut(s) 30, 120, 206, 289, 867
BfaI CTAG 2 cut(s) 869, 1091
BfrI CTTAAG 1 cut(s) 1061
BglII AGATCT 2 cut(s) 418, 817
BisI GCNGC 1 cut(s) 960
BlpI GCTNAGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 961
Bme1390I CCNGG 3 cut(s) 380, 525, 1031
BmgT120I GGNCC 1 cut(s) 992
BmiI GGNNCC 2 cut(s) 559, 745
BmrFI CCNGG 3 cut(s) 380, 525, 1031
BmrI ACTGGG 1 cut(s) 813
BmsI GCATC 1 cut(s) 672
BmuI ACTGGG 1 cut(s) 813
BplI GAGNNNNNCTC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 925
Bpu1102I GCTNAGC 1 cut(s) 181
Bpu14I TTCGAA 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 725
BsaHI GRCGYC 1 cut(s) 710
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 3 cut(s) 378, 379, 916
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 643
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 2 cut(s) 466, 808
BseBI CCWGG 3 cut(s) 380, 525, 1031
BseDI CCNNGG 3 cut(s) 378, 379, 916
BseGI GGATG 2 cut(s) 247, 766
BseLI CCNNNNNNNGG 1 cut(s) 643
BseMII CTCAG 4 cut(s) 77, 131, 201, 939
BseNI ACTGG 2 cut(s) 466, 808
BseXI GCAGC 1 cut(s) 946
BseYI CCCAGC 1 cut(s) 597
Bsh1236I CGCG 2 cut(s) 20, 717
BshFI GGCC 2 cut(s) 655, 993
BshTI ACCGGT 1 cut(s) 154
BsiSI CCGG 1 cut(s) 155
BslFI GGGAC 2 cut(s) 361, 579
BslI CCNNNNNNNGG 1 cut(s) 643
BsmAI GTCTC 5 cut(s) 30, 120, 206, 289, 867
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 2 cut(s) 361, 579
BsnI GGCC 2 cut(s) 655, 993
Bso31I GGTCTC 1 cut(s) 30
Bsp119I TTCGAA 1 cut(s) 28
Bsp143I GATC 3 cut(s) 418, 557, 817
Bsp1720I GCTNAGC 1 cut(s) 181
BspACI CCGC 1 cut(s) 18
BspANI GGCC 2 cut(s) 655, 993
BspCNI CTCAG 4 cut(s) 76, 130, 202, 938
BspFNI CGCG 2 cut(s) 20, 717
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 2 cut(s) 559, 745
BspPI GGATC 2 cut(s) 552, 565
BspT104I TTCGAA 1 cut(s) 28
BspTI CTTAAG 1 cut(s) 1061
BspTNI GGTCTC 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 2 cut(s) 466, 808
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 378, 379, 916
BssMI GATC 3 cut(s) 418, 557, 817
BssNI GRCGYC 1 cut(s) 710
Bst2UI CCWGG 3 cut(s) 380, 525, 1031
Bst4CI ACNGT 3 cut(s) 217, 679, 828
Bst6I CTCTTC 3 cut(s) 584, 852, 973
BstACI GRCGYC 1 cut(s) 710
BstAFI CTTAAG 1 cut(s) 1061
BstAPI GCANNNNNTGC 1 cut(s) 195
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 70, 137, 187
BstDEI CTNAG 9 cut(s) 63, 117, 181, 210, 422, 650, 770, 853, 925
BstDSI CCRYGG 1 cut(s) 916
BstF5I GGATG 2 cut(s) 247, 766
BstFNI CGCG 2 cut(s) 20, 717
BstKTI GATC 3 cut(s) 421, 560, 820
BstMAI GTCTC 5 cut(s) 30, 120, 206, 289, 867
BstMBI GATC 3 cut(s) 418, 557, 817
BstMWI GCNNNNNNNGC 4 cut(s) 186, 195, 229, 314
BstNI CCWGG 3 cut(s) 380, 525, 1031
BstNSI RCATGY 3 cut(s) 189, 522, 880
BstSCI CCNGG 3 cut(s) 378, 523, 1029
BstUI CGCG 2 cut(s) 20, 717
BstV1I GCAGC 1 cut(s) 946
BstX2I RGATCY 3 cut(s) 418, 557, 817
BstYI RGATCY 3 cut(s) 418, 557, 817
BsuRI GGCC 2 cut(s) 655, 993
BtgI CCRYGG 1 cut(s) 916
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 2 cut(s) 247, 766
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 4 cut(s) 196, 591, 684, 798
Cac8I GCNNGC 3 cut(s) 70, 137, 187
CciI TCATGA 1 cut(s) 450
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 992
CseI GACGC 2 cut(s) 31, 699
Csp6I GTAC 2 cut(s) 460, 566
CspAI ACCGGT 1 cut(s) 154
CviAII CATG 6 cut(s) 186, 451, 505, 519, 671, 877
CviQI GTAC 2 cut(s) 460, 566
DdeI CTNAG 9 cut(s) 63, 117, 181, 210, 422, 650, 770, 853, 925
DpnI GATC 3 cut(s) 420, 559, 819
DpnII GATC 3 cut(s) 418, 557, 817
Eam1104I CTCTTC 3 cut(s) 584, 852, 973
EarI CTCTTC 3 cut(s) 584, 852, 973
Eco31I GGTCTC 1 cut(s) 30
Eco57I CTGAAG 3 cut(s) 326, 567, 988
EcoO109I RGGNCCY 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 378, 523, 1029
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 6 cut(s) 189, 454, 508, 522, 674, 880
FaqI GGGAC 2 cut(s) 361, 579
FatI CATG 6 cut(s) 185, 450, 504, 518, 670, 876
Fnu4HI GCNGC 1 cut(s) 960
FokI GGATG 2 cut(s) 254, 773
Fsp4HI GCNGC 1 cut(s) 960
FspBI CTAG 2 cut(s) 869, 1091
GluI GCNGC 1 cut(s) 960
GsaI CCCAGC 1 cut(s) 601
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 2 cut(s) 655, 993
HapII CCGG 1 cut(s) 155
HgaI GACGC 2 cut(s) 31, 699
Hin1I GRCGYC 1 cut(s) 710
Hin1II CATG 6 cut(s) 189, 454, 508, 522, 674, 880
HincII GTYRAC 3 cut(s) 471, 798, 882
HindII GTYRAC 3 cut(s) 471, 798, 882
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 6 cut(s) 30, 434, 592, 788, 860, 912
HpaII CCGG 1 cut(s) 155
Hpy166II GTNNAC 5 cut(s) 471, 667, 798, 882, 1000
Hpy188I TCNGA 8 cut(s) 7, 35, 109, 781, 822, 953, 1007, 1024
Hpy188III TCNNGA 2 cut(s) 303, 451
Hpy8I GTNNAC 5 cut(s) 471, 667, 798, 882, 1000
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 1 cut(s) 983
HpyCH4III ACNGT 3 cut(s) 217, 679, 828
HpyCH4V TGCA 7 cut(s) 72, 125, 189, 198, 317, 411, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 195, 229, 314
HpyF3I CTNAG 9 cut(s) 63, 117, 181, 210, 422, 650, 770, 853, 925
Hsp92I GRCGYC 1 cut(s) 710
Hsp92II CATG 6 cut(s) 189, 454, 508, 522, 674, 880
Kzo9I GATC 3 cut(s) 418, 557, 817
LmnI GCTCC 2 cut(s) 61, 743
Lsp1109I GCAGC 1 cut(s) 946
LweI GCATC 1 cut(s) 672
MaeI CTAG 2 cut(s) 869, 1091
MaeIII GTNAC 4 cut(s) 673, 711, 893, 1036
MalI GATC 3 cut(s) 420, 559, 819
MboI GATC 3 cut(s) 418, 557, 817
MboII GAAGA 4 cut(s) 573, 601, 869, 990
MfeI CAATTG 2 cut(s) 140, 606
MflI RGATCY 3 cut(s) 418, 557, 817
MluCI AATT 8 cut(s) 140, 162, 356, 446, 606, 945, 984, 1018
MlyI GAGTC 3 cut(s) 601, 869, 921
MnlI CCTC 7 cut(s) 70, 115, 700, 751, 775, 841, 1039
MseI TTAA 7 cut(s) 42, 159, 372, 621, 830, 1014, 1062
MslI CAYNNNNRTG 1 cut(s) 517
MspCI CTTAAG 1 cut(s) 1061
MspI CCGG 1 cut(s) 155
MspR9I CCNGG 3 cut(s) 380, 525, 1031
MunI CAATTG 2 cut(s) 140, 606
MvaI CCWGG 3 cut(s) 380, 525, 1031
MvnI CGCG 2 cut(s) 20, 717
MwoI GCNNNNNNNGC 4 cut(s) 186, 195, 229, 314
NdeII GATC 3 cut(s) 418, 557, 817
NlaIII CATG 6 cut(s) 189, 454, 508, 522, 674, 880
NlaIV GGNNCC 2 cut(s) 559, 745
NmuCI GTSAC 3 cut(s) 711, 893, 1036
NspI RCATGY 3 cut(s) 189, 522, 880
NspV TTCGAA 1 cut(s) 28
PaeI GCATGC 1 cut(s) 189
PagI TCATGA 1 cut(s) 450
PasI CCCWGGG 1 cut(s) 379
PfeI GAWTC 3 cut(s) 30, 434, 788
PflMI CCANNNNNTGG 1 cut(s) 643
PfoI TCCNGGA 1 cut(s) 1029
PinAI ACCGGT 1 cut(s) 154
PkrI GCNGC 1 cut(s) 961
PleI GAGTC 3 cut(s) 600, 868, 920
PpsI GAGTC 3 cut(s) 600, 868, 920
Psp6I CCWGG 3 cut(s) 378, 523, 1029
PspFI CCCAGC 1 cut(s) 597
PspGI CCWGG 3 cut(s) 378, 523, 1029
PspN4I GGNNCC 2 cut(s) 559, 745
PspPI GGNCC 1 cut(s) 992
PsrI GAACNNNNNNTAC 2 cut(s) 1041, 1073
PsuI RGATCY 3 cut(s) 418, 557, 817
RsaI GTAC 2 cut(s) 461, 567
RsaNI GTAC 2 cut(s) 460, 566
RseI CAYNNNNRTG 1 cut(s) 517
SaqAI TTAA 7 cut(s) 42, 159, 372, 621, 830, 1014, 1062
SatI GCNGC 1 cut(s) 960
Sau3AI GATC 3 cut(s) 418, 557, 817
Sau96I GGNCC 1 cut(s) 992
SchI GAGTC 3 cut(s) 601, 869, 921
ScrFI CCNGG 3 cut(s) 380, 525, 1031
SfaNI GCATC 1 cut(s) 672
SfuI TTCGAA 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 517
SmlI CTYRAG 2 cut(s) 704, 1061
SmoI CTYRAG 2 cut(s) 704, 1061
SphI GCATGC 1 cut(s) 189
Sse9I AATT 8 cut(s) 140, 162, 356, 446, 606, 945, 984, 1018
SsiI CCGC 1 cut(s) 18
SspI AATATT 1 cut(s) 259
SspMI CTAG 2 cut(s) 869, 1091
StyD4I CCNGG 3 cut(s) 378, 523, 1029
TaaI ACNGT 3 cut(s) 217, 679, 828
TaqI TCGA 1 cut(s) 28
TasI AATT 8 cut(s) 140, 162, 356, 446, 606, 945, 984, 1018
TatI WGTACW 1 cut(s) 565
TfiI GAWTC 3 cut(s) 30, 434, 788
Tru1I TTAA 7 cut(s) 42, 159, 372, 621, 830, 1014, 1062
Tru9I TTAA 7 cut(s) 42, 159, 372, 621, 830, 1014, 1062
TscAI CASTG 4 cut(s) 196, 591, 684, 798
TseFI GTSAC 3 cut(s) 711, 893, 1036
TseI GCWGC 1 cut(s) 959
Tsp45I GTSAC 3 cut(s) 711, 893, 1036
TspDTI ATGAA 5 cut(s) 311, 439, 459, 495, 501
TspGWI ACGGA 3 cut(s) 905, 912, 924
TspRI CASTG 4 cut(s) 196, 591, 684, 798
Van91I CCANNNNNTGG 1 cut(s) 643
Vha464I CTTAAG 1 cut(s) 1061
XapI RAATTY 3 cut(s) 162, 446, 1018
XceI RCATGY 3 cut(s) 189, 522, 880
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XspI CTAG 2 cut(s) 869, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.