MD11G1000700.v1.1

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0017552
Physical Location & Seq
Forward (+)
101562 .. 102558
997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1000700.v1.1.491

Sequence Viewer

Length: 969 bp
ATGAGAGACCTGTCCTTGTTTCTAATCAAGAACTCTCTTGGAGCCAAGATGAAGAAAGGGTTGAGAAACTTTTGCAACGATGATGGCTCAACCTCTACCCTCAACCAACATCACAAGACCACCTGCACCGATGCTGCTGCTGCTACCTCCTCTAATCCTGCACCACTTCCGGTTGTTCGCTCTCCTACTACTGATTATAATTACTATTTGCAGGAAAATAATACCAGCAGTATTAATGATTCAGATCATGCTCTTCATCAGCCGCCGACGTTGGAGGAGATGATACTGAGGTTAGATTTAGAAGAGGAGATTGCGAGATCATCAAAGTATTCAAAACCGAAAGATTATAACAAGTACAAGTACTACAAGGGAAGAATGTCTTGCGTTAACAATTCTGATATTTTACGGTCTGCAAGGAATGCATTAAATCAGTACCCTCGCTTCTCGCTCGATGGCAAGGATGCCATGTATCGCTCCTCCTTCAGAAACAGCTTGGATCATGATTTTGAGCATGATCATACAAATGCTTTTACAAGGACGAAACTGCAGTACTGTAGGCTGCCAGCAACTGTAGCAGGGGAGAGGGTGAAGTGGTGCAAACCAGGAGTAGTAGCTAAGTTGATGGGGCTAGAGGCCATGCCAGTACCTGTGATGAGTAGTACTGGTAGTAAATTAGACATTGCTAGCGGTAGTATCATCGATGTCATGAACATGAAGAAACGGAATCTCATGATCAGGAGGAGAGCTCATGATCATCAAAGACATGAATTGGGGAGAAGACTTGTTAGTAGTTATATGGATATCAATGGCAACGGTAGGCAATGTGATCACAGAATTTTCAATAAGAAAGCTGCAGCAGCTGGCAAGAGGACTACTGCTGCTTTCTCCTCATGCACCACTTCCAAAACTACAAGTGCAGGTTACAATAATAATTACCATATCATGAAGGCAGGTGTTGCCAACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

323

Amino Acids

36.19

Weight (kDa)

9.62

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 198 - 214 4.8e-06 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26415
fragaria_vesca FvH4_3g45980
malus_domestica MD11G1000700.v1.1
prunus_persica Prupe.6G001000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0084091
rosa_laevigata RLG00000037081
rosa_roxburghii Rroxscaffold_1G00000550
rosa_samantha Rh5AG541700 Rh5BG567200 Rh5CG589000 Rh5DG575400
rosa_wichuraiana Rw5G050380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 198, 348
AarI CACCTGC 2 cut(s) 131, 941
Acc36I ACCTGC 3 cut(s) 131, 908, 941
AciI CCGC 2 cut(s) 263, 687
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 1 cut(s) 834
AcuI CTGAAG 1 cut(s) 466
AfaI GTAC 6 cut(s) 356, 362, 434, 551, 645, 661
AgsI TTSAA 2 cut(s) 333, 841
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 6 cut(s) 492, 614, 746, 851, 860, 966
AluI AGCT 6 cut(s) 492, 614, 746, 851, 860, 966
Alw21I GWGCWC 1 cut(s) 748
AlwI GGATC 1 cut(s) 504
AlwNI CAGNNNCTG 3 cut(s) 569, 647, 860
AoxI GGCC 1 cut(s) 633
ApeKI GCWGC 8 cut(s) 134, 137, 140, 559, 851, 854, 857, 878
ApoI RAATTY 1 cut(s) 834
AseI ATTAAT 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 598
AsuNHI GCTAGC 1 cut(s) 683
BaeI ACNNNNGTAYC 2 cut(s) 275, 308
BanII GRGCYC 1 cut(s) 748
BarI GAAGNNNNNNTAC 2 cut(s) 425, 457
BbsI GAAGAC 1 cut(s) 784
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 8 cut(s) 121, 124, 127, 546, 838, 865, 866, 869
BccI CCATC 3 cut(s) 77, 446, 616
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 1 cut(s) 603
BclI TGATCA 4 cut(s) 514, 732, 751, 826
BfaI CTAG 3 cut(s) 629, 684, 967
BfmI CTRYAG 4 cut(s) 545, 553, 570, 852
BfuAI ACCTGC 3 cut(s) 131, 908, 941
BisI GCNGC 9 cut(s) 135, 138, 141, 263, 560, 852, 855, 858, 879
BlsI GCNGC 9 cut(s) 136, 139, 142, 264, 561, 853, 856, 859, 880
BmcAI AGTACT 3 cut(s) 362, 551, 661
Bme1390I CCNGG 1 cut(s) 603
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 603
BmsI GCATC 2 cut(s) 121, 451
BmtI GCTAGC 1 cut(s) 687
BpiI GAAGAC 1 cut(s) 784
BplI GAGNNNNNCTC 2 cut(s) 730, 762
Bsa29I ATCGAT 1 cut(s) 699
BsaBI GATNNNNATC 1 cut(s) 243
BsaWI WCCGGW 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 641, 667
Bse3DI GCAATG 2 cut(s) 678, 827
Bse8I GATNNNNATC 1 cut(s) 243
BseBI CCWGG 1 cut(s) 603
BseCI ATCGAT 1 cut(s) 699
BseGI GGATG 1 cut(s) 466
BseJI GATNNNNATC 1 cut(s) 243
BseMI GCAATG 2 cut(s) 678, 827
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 2 cut(s) 641, 667
BseRI GAGGAG 6 cut(s) 139, 290, 320, 466, 754, 877
BseXI GCAGC 8 cut(s) 121, 124, 127, 546, 838, 865, 866, 869
BsgI GTGCAG 3 cut(s) 109, 144, 936
BshFI GGCC 1 cut(s) 635
BshVI ATCGAT 1 cut(s) 699
BsiHKAI GWGCWC 1 cut(s) 748
BsiSI CCGG 1 cut(s) 170
BsmI GAATGC 1 cut(s) 424
BsnI GGCC 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 748
Bsp143I GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
BspACI CCGC 2 cut(s) 263, 687
BspANI GGCC 1 cut(s) 635
BspCNI CTCAG 1 cut(s) 279
BspDI ATCGAT 1 cut(s) 699
BspHI TCATGA 5 cut(s) 499, 705, 729, 748, 942
BspLI GGNNCC 1 cut(s) 43
BspMAI CTGCAG 2 cut(s) 549, 856
BspMI ACCTGC 3 cut(s) 131, 908, 941
BspOI GCTAGC 1 cut(s) 687
BspPI GGATC 1 cut(s) 504
BspQI GCTCTTC 1 cut(s) 258
BsrDI GCAATG 2 cut(s) 678, 827
BsrI ACTGG 2 cut(s) 641, 667
BssMI GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 4 cut(s) 408, 554, 571, 815
Bst6I CTCTTC 2 cut(s) 258, 297
BstAPI GCANNNNNTGC 2 cut(s) 419, 956
BstC8I GCNNGC 3 cut(s) 564, 685, 862
BstDEI CTNAG 2 cut(s) 287, 615
BstF5I GGATG 1 cut(s) 466
BstKTI GATC 7 cut(s) 247, 320, 499, 517, 735, 754, 829
BstMBI GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
BstMWI GCNNNNNNNGC 5 cut(s) 140, 419, 572, 857, 956
BstNI CCWGG 1 cut(s) 603
BstSCI CCNGG 1 cut(s) 601
BstSFI CTRYAG 4 cut(s) 545, 553, 570, 852
BstV1I GCAGC 8 cut(s) 121, 124, 127, 546, 838, 865, 866, 869
BstV2I GAAGAC 1 cut(s) 784
Bsu15I ATCGAT 1 cut(s) 699
BsuRI GGCC 1 cut(s) 635
BsuTUI ATCGAT 1 cut(s) 699
BtsCI GGATG 1 cut(s) 466
BveI ACCTGC 3 cut(s) 131, 908, 941
Cac8I GCNNGC 3 cut(s) 564, 685, 862
CaiI CAGNNNCTG 3 cut(s) 569, 647, 860
CciI TCATGA 5 cut(s) 499, 705, 729, 748, 942
ClaI ATCGAT 1 cut(s) 699
Csp6I GTAC 6 cut(s) 355, 361, 433, 550, 644, 660
CviQI GTAC 6 cut(s) 355, 361, 433, 550, 644, 660
DdeI CTNAG 2 cut(s) 287, 615
DpnI GATC 7 cut(s) 246, 319, 498, 516, 734, 753, 828
DpnII GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
Eam1104I CTCTTC 2 cut(s) 258, 297
EarI CTCTTC 2 cut(s) 258, 297
Ecl136II GAGCTC 1 cut(s) 746
Eco24I GRGCYC 1 cut(s) 748
Eco32I GATATC 1 cut(s) 802
Eco53kI GAGCTC 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 746
EcoRII CCWGG 1 cut(s) 601
EcoRV GATATC 1 cut(s) 802
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 748
FalI AAGNNNNNCTT 2 cut(s) 364, 396
FbaI TGATCA 4 cut(s) 514, 732, 751, 826
Fnu4HI GCNGC 9 cut(s) 135, 138, 141, 263, 560, 852, 855, 858, 879
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 9 cut(s) 135, 138, 141, 263, 560, 852, 855, 858, 879
FspBI CTAG 3 cut(s) 629, 684, 967
GluI GCNGC 9 cut(s) 135, 138, 141, 263, 560, 852, 855, 858, 879
HaeIII GGCC 1 cut(s) 635
HapII CCGG 1 cut(s) 170
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 2 cut(s) 239, 724
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 3 cut(s) 244, 397, 485
Hpy188III TCNNGA 7 cut(s) 28, 500, 706, 730, 736, 749, 943
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 1 cut(s) 271
HpyAV CCTTC 2 cut(s) 490, 940
HpyCH4III ACNGT 4 cut(s) 408, 554, 571, 815
HpyCH4IV ACGT 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 419, 572, 857, 956
HpyF3I CTNAG 2 cut(s) 287, 615
HpySE526I ACGT 1 cut(s) 269
Ksp22I TGATCA 4 cut(s) 514, 732, 751, 826
KspAI GTTAAC 1 cut(s) 388
Kzo9I GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 2 cut(s) 41, 479
Lsp1109I GCAGC 8 cut(s) 121, 124, 127, 546, 838, 865, 866, 869
LweI GCATC 2 cut(s) 121, 451
MaeI CTAG 3 cut(s) 629, 684, 967
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 1 cut(s) 920
MalI GATC 7 cut(s) 246, 319, 498, 516, 734, 753, 828
MboI GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
MboII GAAGA 6 cut(s) 64, 245, 314, 384, 727, 789
MhlI GDGCHC 1 cut(s) 748
MluCI AATT 6 cut(s) 199, 391, 671, 767, 834, 931
MmeI TCCRAC 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 424
MseI TTAA 3 cut(s) 234, 387, 425
MslI CAYNNNNRTG 2 cut(s) 522, 710
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 603
Mva1269I GAATGC 1 cut(s) 424
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 5 cut(s) 140, 419, 572, 857, 956
NdeII GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
NheI GCTAGC 1 cut(s) 683
NlaIV GGNNCC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 424
PagI TCATGA 5 cut(s) 499, 705, 729, 748, 942
PaqCI CACCTGC 2 cut(s) 131, 941
PciSI GCTCTTC 1 cut(s) 258
PctI GAATGC 1 cut(s) 424
PfeI GAWTC 2 cut(s) 239, 724
PkrI GCNGC 9 cut(s) 136, 139, 142, 264, 561, 853, 856, 859, 880
PshBI ATTAAT 1 cut(s) 234
PsiI TTATAA 2 cut(s) 198, 348
Psp124BI GAGCTC 1 cut(s) 748
Psp6I CCWGG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 1 cut(s) 43
PstI CTGCAG 2 cut(s) 549, 856
PstNI CAGNNNCTG 3 cut(s) 569, 647, 860
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 6 cut(s) 356, 362, 434, 551, 645, 661
RsaNI GTAC 6 cut(s) 355, 361, 433, 550, 644, 660
RseI CAYNNNNRTG 2 cut(s) 522, 710
SacI GAGCTC 1 cut(s) 748
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 3 cut(s) 234, 387, 425
SatI GCNGC 9 cut(s) 135, 138, 141, 263, 560, 852, 855, 858, 879
Sau3AI GATC 7 cut(s) 244, 317, 496, 514, 732, 751, 826
ScaI AGTACT 3 cut(s) 362, 551, 661
ScrFI CCNGG 1 cut(s) 603
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 2 cut(s) 121, 451
SfcI CTRYAG 4 cut(s) 545, 553, 570, 852
SmiMI CAYNNNNRTG 2 cut(s) 522, 710
Sse9I AATT 6 cut(s) 199, 391, 671, 767, 834, 931
SsiI CCGC 2 cut(s) 263, 687
SspMI CTAG 3 cut(s) 629, 684, 967
SstI GAGCTC 1 cut(s) 748
StyD4I CCNGG 1 cut(s) 601
TaaI ACNGT 4 cut(s) 408, 554, 571, 815
TaiI ACGT 1 cut(s) 272
TaqI TCGA 2 cut(s) 450, 699
TasI AATT 6 cut(s) 199, 391, 671, 767, 834, 931
TatI WGTACW 4 cut(s) 354, 360, 549, 659
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 2 cut(s) 239, 724
Tru1I TTAA 3 cut(s) 234, 387, 425
Tru9I TTAA 3 cut(s) 234, 387, 425
TseI GCWGC 8 cut(s) 134, 137, 140, 559, 851, 854, 857, 878
TspDTI ATGAA 6 cut(s) 65, 245, 722, 728, 780, 959
TspGWI ACGGA 1 cut(s) 736
VspI ATTAAT 1 cut(s) 234
XapI RAATTY 1 cut(s) 834
XspI CTAG 3 cut(s) 629, 684, 967
ZrmI AGTACT 3 cut(s) 362, 551, 661
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.