MD11G1047600.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008578
Physical Location & Seq
Forward (+)
4140857 .. 4143346
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1047600.v1.1.491

Sequence Viewer

Length: 1818 bp
ATGGGTTTGGAGAGACTGCACATCTGCCTATTAATAAAGGAGATTCTTCTCAAGCTGGACATCGAATCTCTTTCCTCGCTTGCTTGCGTCAGCAAAGCCATGAACTTCTCAGTCTCTCGGGCCCTTCCCTTTGTCTCCTCTCTCGATCTCTCCGCATTTTATCCGGATGCGCAGATTTTGAGTGGCATTGTTGGTGGCTGCACAGGCCTCATCAGCCTCACCGTTAACTGCCTCCGCCTCGAAAATTCGGCAATCAGAAATGTTCTGGGGCCACATATCCAAGAACTGAATTTGTTGAGCTGCTCTATGCTGTCCTATCAAGTCTTTGCTTCCATTGGACAGACCTGCCCTAATCTGAGGGTGCTTATGCTAGAATTGGTAGACTCGGATAAGGAACTTAAGTTTTACCTGGACTGCATGCTTAATGGATGCTTGTTCTTGGAGTCTTTATCTCTTAAGATTCGAGGGAGAGATTTCGATTCAACTGCTTTTCAGTCCATTGATTTCGTCCTACCTAGTACTCTTAAAAGCATGAAGTTGCAGTCGGTGCTTGAGCATGACGCGATTCGTCTAGTTGATCAAAGTAGAGTTGGTGCTGGCAGGAATAATGTGCAAAGAGCTCATATCAGCTTTCCCATCTCTCCATTGTCATCTGGATTGACATTGCAGCGCTTGTCCCTCGTCTTAGATGTTATATCTGATCAGCTGATCATTGTCATTGCCCGCAGTCTTCCCCATTTGGTAGAGCTAGATCTTGAAGATAGACCACATATGGAACCACTTCCGAACCTCGACTTGACAAACACCGGGCTTCAATATGTGGCATCCTGTCATCATTTGATTGGATTGTCTCTTGTACGAAGGAGACAAAACTACCAAATTTCATTCAAAAGAGTAACTGATGTGGGCATGTTTCTTCTTTCTGAGGGCTGCAAAGGTCTACAGTCAGTGAGACTCTGTGGGTTCTCCAAAGTCAGTGACGCAGGCTATACATCGATCTTACAATCATGTCTGAACTTGAAGAAATTTGAAGCTCGAAATGCCTTCCGATTGTCAGACTTGGCCTTTCTTAATGTTAATGAGTTCCCATGTTCCCTTGTTGAGGTAAAATTGCTGTCGTGTAGTTTTATAACCAGTGAAGCTGTGAAGCAGCTGACCTGTTCCAGCACCTTGGAGGTGCTTTACCTCTGTGGCTGTAAGAGCATAGCAGATTCCTGCCTCAGTTCCATCTCATGTCTGCGTAGCTTAACCACGCTGAATCTCACAGGAGCTGATATCACTGATTACGGCTTGTCCATTCTTGGCCAGGGGACTCCACCCATTACTCATTTGTGTCTCAGATACTGTACAAGGGTGACCGACGAAGGAGTTTCTCTTTTATTCGATAGTGGAGGCACAATTGGCAAAACTTTGTCGTCCCTGGATTTAGGATATATCCCGAATATATCAGATAAAGCCGTTCTTACAATTGCCATGGTCGGTACGGAGATTACAGAGTTGTGTTTAAGATGTTGCCTCTCTGTGACTGATTCTTCCCTGGAAGTTTTGGCTGCAAGGAAAAGGTTCCGAGACGAATGCAAACCACTTCGGAGGCTGGACCTTACAAGTTGCAGTGGTTTGTCTATTGATTCATTGGTGTTGCTGAAGAGGCCTTCCTTCGCGGGACTACACTGGCTTGGTATTGGGAATACTTATCTGTCTAGCAAAGGATACGGAGCTCTGAGTGATATTTGTAACCAAAAGCCATGGCTGAAGATATGTCTGGAATGTTGTGAAATGGGATGTTATGATGGATGGCAATTCCATGGAGGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

606

Amino Acids

66.37

Weight (kDa)

6.8

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 48 - 596 2e-185 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1130
Acc16I TGCGCA 1 cut(s) 171
Acc36I ACCTGC 1 cut(s) 353
AccI GTMKAC 2 cut(s) 381, 940
AccII CGCG 2 cut(s) 563, 1661
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 4 cut(s) 153, 235, 724, 1661
AcoI YGGCCR 1 cut(s) 1303
AcsI RAATTY 4 cut(s) 244, 289, 879, 1025
AcuI CTGAAG 2 cut(s) 1664, 1772
AfaI GTAC 4 cut(s) 520, 858, 1348, 1483
AfeI AGCGCT 1 cut(s) 671
AfiI CCNNNNNNNGG 1 cut(s) 1102
AflII CTTAAG 2 cut(s) 398, 455
AgsI TTSAA 6 cut(s) 483, 758, 815, 889, 1021, 1031
AjnI CCWGG 4 cut(s) 408, 1305, 1419, 1536
Alw21I GWGCWC 2 cut(s) 622, 1720
Alw26I GTCTC 8 cut(s) 7, 118, 139, 855, 859, 946, 1340, 1563
AlwNI CAGNNNCTG 2 cut(s) 1271, 1344
Ama87I CYCGRG 1 cut(s) 117
Aor13HI TCCGGA 1 cut(s) 163
Aor51HI AGCGCT 1 cut(s) 671
AoxI GGCC 6 cut(s) 120, 205, 269, 1062, 1303, 1649
ApaI GGGCCC 1 cut(s) 124
ApeKI GCWGC 6 cut(s) 198, 300, 667, 930, 1150, 1550
ApoI RAATTY 4 cut(s) 244, 289, 879, 1025
AseI ATTAAT 1 cut(s) 32
Asp700I GAANNNNTTC 2 cut(s) 780, 1562
AspLEI GCGC 2 cut(s) 172, 672
AspS9I GGNCC 4 cut(s) 120, 121, 269, 1597
AsuC2I CCSGG 1 cut(s) 808
AsuHPI GGTGA 2 cut(s) 211, 1366
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 1 cut(s) 1597
BaeGI GKGCMC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 1305
BanII GRGCYC 3 cut(s) 124, 622, 1720
BbsI GAAGAC 1 cut(s) 722
Bbv12I GWGCWC 2 cut(s) 622, 1720
BbvI GCAGC 6 cut(s) 185, 287, 679, 917, 1162, 1537
BccI CCATC 4 cut(s) 644, 1235, 1784, 1788
BceAI ACGGC 2 cut(s) 1303, 1442
BcgI CGANNNNNNTGC 4 cut(s) 467, 501, 1557, 1591
BciT130I CCWGG 4 cut(s) 410, 1307, 1421, 1538
BciVI GTATCC 1 cut(s) 1703
BclI TGATCA 3 cut(s) 577, 700, 708
BcnI CCSGG 1 cut(s) 808
BcoDI GTCTC 8 cut(s) 7, 118, 139, 855, 859, 946, 1340, 1563
BfaI CTAG 5 cut(s) 371, 516, 572, 749, 1701
BfmI CTRYAG 1 cut(s) 941
BfoI RGCGCY 1 cut(s) 673
BfrI CTTAAG 2 cut(s) 398, 455
BfuAI ACCTGC 1 cut(s) 353
BfuI GTATCC 1 cut(s) 1703
BglII AGATCT 1 cut(s) 751
BisI GCNGC 6 cut(s) 199, 301, 668, 931, 1151, 1551
BlsI GCNGC 6 cut(s) 200, 302, 669, 932, 1152, 1552
BmcAI AGTACT 1 cut(s) 520
Bme1390I CCNGG 5 cut(s) 410, 808, 1307, 1421, 1538
Bme18I GGWCC 1 cut(s) 1597
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 4 cut(s) 120, 121, 269, 1597
BmiI GGNNCC 4 cut(s) 122, 270, 777, 1565
BmrFI CCNGG 5 cut(s) 410, 808, 1307, 1421, 1538
BmsI GCATC 3 cut(s) 157, 419, 833
BpiI GAAGAC 1 cut(s) 722
BpuEI CTTGAG 2 cut(s) 35, 572
BpuMI CCSGG 1 cut(s) 808
Bsa29I ATCGAT 1 cut(s) 995
BsaJI CCNNGG 7 cut(s) 1170, 1306, 1419, 1473, 1536, 1745, 1804
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 1102
Bse1I ACTGG 2 cut(s) 1134, 1676
Bse3DI GCAATG 2 cut(s) 662, 717
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 4 cut(s) 410, 1307, 1421, 1538
BseCI ATCGAT 1 cut(s) 995
BseDI CCNNGG 7 cut(s) 1170, 1306, 1419, 1473, 1536, 1745, 1804
BseGI GGATG 5 cut(s) 172, 434, 824, 1787, 1799
BseLI CCNNNNNNNGG 1 cut(s) 1102
BseMI GCAATG 2 cut(s) 662, 717
BseMII CTCAG 6 cut(s) 123, 347, 915, 1234, 1351, 1712
BseNI ACTGG 2 cut(s) 1134, 1676
BseRI GAGGAG 1 cut(s) 127
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 6 cut(s) 185, 287, 679, 917, 1162, 1537
BsgI GTGCAG 1 cut(s) 184
Bsh1236I CGCG 2 cut(s) 563, 1661
BshFI GGCC 6 cut(s) 122, 207, 271, 1064, 1305, 1651
BshVI ATCGAT 1 cut(s) 995
BsiHKAI GWGCWC 2 cut(s) 622, 1720
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 2 cut(s) 164, 807
BslFI GGGAC 4 cut(s) 661, 1324, 1402, 1677
BslI CCNNNNNNNGG 1 cut(s) 1102
BsmAI GTCTC 8 cut(s) 7, 118, 139, 855, 859, 946, 1340, 1563
BsmBI CGTCTC 1 cut(s) 1563
BsmFI GGGAC 4 cut(s) 661, 1324, 1402, 1677
BsmI GAATGC 1 cut(s) 1580
BsnI GGCC 6 cut(s) 122, 207, 271, 1064, 1305, 1651
BsoBI CYCGRG 1 cut(s) 117
Bsp120I GGGCCC 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 124, 622, 1720
Bsp13I TCCGGA 1 cut(s) 163
Bsp1407I TGTACA 1 cut(s) 1346
Bsp143I GATC 6 cut(s) 145, 577, 700, 708, 751, 996
Bsp19I CCATGG 3 cut(s) 1473, 1745, 1804
BspACI CCGC 4 cut(s) 153, 235, 724, 1661
BspANI GGCC 6 cut(s) 122, 207, 271, 1064, 1305, 1651
BspCNI CTCAG 6 cut(s) 122, 348, 916, 1233, 1350, 1713
BspDI ATCGAT 1 cut(s) 995
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 2 cut(s) 563, 1661
BspLI GGNNCC 4 cut(s) 122, 270, 777, 1565
BspMI ACCTGC 1 cut(s) 353
BspTI CTTAAG 2 cut(s) 398, 455
BsrDI GCAATG 2 cut(s) 662, 717
BsrGI TGTACA 1 cut(s) 1346
BsrI ACTGG 2 cut(s) 1134, 1676
BssECI CCNNGG 7 cut(s) 1170, 1306, 1419, 1473, 1536, 1745, 1804
BssMI GATC 6 cut(s) 145, 577, 700, 708, 751, 996
BssT1I CCWWGG 4 cut(s) 1170, 1473, 1745, 1804
Bst2UI CCWGG 4 cut(s) 410, 1307, 1421, 1538
Bst4CI ACNGT 3 cut(s) 223, 945, 1346
Bst6I CTCTTC 1 cut(s) 1640
BstAFI CTTAAG 2 cut(s) 398, 455
BstAPI GCANNNNNTGC 1 cut(s) 547
BstAUI TGTACA 1 cut(s) 1346
BstC8I GCNNGC 6 cut(s) 81, 85, 419, 598, 724, 985
BstDEI CTNAG 7 cut(s) 109, 356, 685, 924, 1220, 1337, 1721
BstDSI CCRYGG 3 cut(s) 1473, 1745, 1804
BstEII GGTNACC 1 cut(s) 1354
BstENI CCTNNNNNAGG 1 cut(s) 1100
BstF5I GGATG 5 cut(s) 172, 434, 824, 1787, 1799
BstFNI CGCG 2 cut(s) 563, 1661
BstH2I RGCGCY 1 cut(s) 673
BstHHI GCGC 2 cut(s) 172, 672
BstKTI GATC 6 cut(s) 148, 580, 703, 711, 754, 999
BstMAI GTCTC 8 cut(s) 7, 118, 139, 855, 859, 946, 1340, 1563
BstMBI GATC 6 cut(s) 145, 577, 700, 708, 751, 996
BstMWI GCNNNNNNNGC 7 cut(s) 204, 213, 547, 1040, 1200, 1401, 1648
BstNI CCWGG 4 cut(s) 410, 1307, 1421, 1538
BstNSI RCATGY 2 cut(s) 421, 913
BstPI GGTNACC 1 cut(s) 1354
BstSCI CCNGG 5 cut(s) 408, 806, 1305, 1419, 1536
BstSFI CTRYAG 1 cut(s) 941
BstSLI GKGCMC 1 cut(s) 124
BstUI CGCG 2 cut(s) 563, 1661
BstV1I GCAGC 6 cut(s) 185, 287, 679, 917, 1162, 1537
BstV2I GAAGAC 1 cut(s) 722
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 1171
BstYI RGATCY 1 cut(s) 751
Bsu15I ATCGAT 1 cut(s) 995
BsuI GTATCC 1 cut(s) 1703
BsuRI GGCC 6 cut(s) 122, 207, 271, 1064, 1305, 1651
BsuTUI ATCGAT 1 cut(s) 995
BtgI CCRYGG 3 cut(s) 1473, 1745, 1804
BtsCI GGATG 5 cut(s) 172, 434, 824, 1787, 1799
BtsI GCAGTG 1 cut(s) 1618
BtsIMutI CAGTG 6 cut(s) 954, 982, 1141, 1278, 1618, 1669
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 6 cut(s) 81, 85, 419, 598, 724, 985
CaiI CAGNNNCTG 2 cut(s) 1271, 1344
CfoI GCGC 2 cut(s) 172, 672
Cfr13I GGNCC 4 cut(s) 120, 121, 269, 1597
ClaI ATCGAT 1 cut(s) 995
CseI GACGC 3 cut(s) 76, 569, 989
Csp6I GTAC 4 cut(s) 519, 857, 1347, 1482
CviQI GTAC 4 cut(s) 519, 857, 1347, 1482
DdeI CTNAG 7 cut(s) 109, 356, 685, 924, 1220, 1337, 1721
DpnI GATC 6 cut(s) 147, 579, 702, 710, 753, 998
DpnII GATC 6 cut(s) 145, 577, 700, 708, 751, 996
EaeI YGGCCR 1 cut(s) 1303
Eam1104I CTCTTC 1 cut(s) 1640
EarI CTCTTC 1 cut(s) 1640
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 2 cut(s) 620, 1718
Eco130I CCWWGG 4 cut(s) 1170, 1473, 1745, 1804
Eco147I AGGCCT 2 cut(s) 207, 1651
Eco24I GRGCYC 3 cut(s) 124, 622, 1720
Eco32I GATATC 1 cut(s) 1276
Eco47I GGWCC 1 cut(s) 1597
Eco47III AGCGCT 1 cut(s) 671
Eco53kI GAGCTC 2 cut(s) 620, 1718
Eco57I CTGAAG 2 cut(s) 1664, 1772
Eco88I CYCGRG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 1354
EcoICRI GAGCTC 2 cut(s) 620, 1718
EcoNI CCTNNNNNAGG 1 cut(s) 1100
EcoO109I RGGNCCY 1 cut(s) 121
EcoO65I GGTNACC 1 cut(s) 1354
EcoRII CCWGG 4 cut(s) 408, 1305, 1419, 1536
EcoRV GATATC 1 cut(s) 1276
EcoT14I CCWWGG 4 cut(s) 1170, 1473, 1745, 1804
EcoT38I GRGCYC 3 cut(s) 124, 622, 1720
ErhI CCWWGG 4 cut(s) 1170, 1473, 1745, 1804
Esp3I CGTCTC 1 cut(s) 1563
FalI AAGNNNNNCTT 2 cut(s) 1446, 1478
FaqI GGGAC 4 cut(s) 661, 1324, 1402, 1677
FauI CCCGC 2 cut(s) 731, 1654
FauNDI CATATG 1 cut(s) 771
FbaI TGATCA 3 cut(s) 577, 700, 708
FblI GTMKAC 2 cut(s) 381, 940
Fnu4HI GCNGC 6 cut(s) 199, 301, 668, 931, 1151, 1551
FokI GGATG 5 cut(s) 179, 441, 811, 1794, 1806
FriOI GRGCYC 3 cut(s) 124, 622, 1720
Fsp4HI GCNGC 6 cut(s) 199, 301, 668, 931, 1151, 1551
FspBI CTAG 5 cut(s) 371, 516, 572, 749, 1701
FspI TGCGCA 1 cut(s) 171
GlaI GCGC 2 cut(s) 171, 671
GluI GCNGC 6 cut(s) 199, 301, 668, 931, 1151, 1551
HaeII RGCGCY 1 cut(s) 673
HaeIII GGCC 6 cut(s) 122, 207, 271, 1064, 1305, 1651
HapII CCGG 2 cut(s) 164, 807
HgaI GACGC 3 cut(s) 76, 569, 989
HhaI GCGC 2 cut(s) 172, 672
Hin6I GCGC 2 cut(s) 170, 670
HinP1I GCGC 2 cut(s) 170, 670
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 2 cut(s) 164, 807
HphI GGTGA 2 cut(s) 211, 1366
Hpy166II GTNNAC 3 cut(s) 226, 382, 941
Hpy188III TCNNGA 6 cut(s) 143, 164, 654, 755, 1438, 1763
Hpy8I GTNNAC 3 cut(s) 226, 382, 941
Hpy99I CGWCG 1 cut(s) 1364
HpyAV CCTTC 6 cut(s) 134, 855, 1054, 1358, 1662, 1666
HpyCH4III ACNGT 3 cut(s) 223, 945, 1346
HpyF10VI GCNNNNNNNGC 7 cut(s) 204, 213, 547, 1040, 1200, 1401, 1648
HpyF3I CTNAG 7 cut(s) 109, 356, 685, 924, 1220, 1337, 1721
HspAI GCGC 2 cut(s) 170, 670
Kpn2I TCCGGA 1 cut(s) 163
Ksp22I TGATCA 3 cut(s) 577, 700, 708
KspAI GTTAAC 1 cut(s) 226
Kzo9I GATC 6 cut(s) 145, 577, 700, 708, 751, 996
LmnI GCTCC 2 cut(s) 1268, 1715
Lsp1109I GCAGC 6 cut(s) 185, 287, 679, 917, 1162, 1537
LweI GCATC 3 cut(s) 157, 419, 833
MaeI CTAG 5 cut(s) 371, 516, 572, 749, 1701
MaeIII GTNAC 5 cut(s) 895, 977, 1354, 1522, 1733
MalI GATC 6 cut(s) 147, 579, 702, 710, 753, 998
MboI GATC 6 cut(s) 145, 577, 700, 708, 751, 996
MboII GAAGA 8 cut(s) 38, 722, 770, 908, 1033, 1524, 1657, 1765
MfeI CAATTG 2 cut(s) 1398, 1467
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 3 cut(s) 124, 622, 1720
MlsI TGGCCA 1 cut(s) 1305
MluCI AATT 9 cut(s) 244, 289, 374, 879, 1025, 1109, 1398, 1467, 1799
MluNI TGGCCA 1 cut(s) 1305
MlyI GAGTC 4 cut(s) 377, 452, 948, 1306
Mox20I TGGCCA 1 cut(s) 1305
MroI TCCGGA 1 cut(s) 163
MroXI GAANNNNTTC 2 cut(s) 780, 1562
MscI TGGCCA 1 cut(s) 1305
Msp20I TGGCCA 1 cut(s) 1305
MspA1I CMGCKG 2 cut(s) 706, 1153
MspCI CTTAAG 2 cut(s) 398, 455
MspI CCGG 2 cut(s) 164, 807
MspR9I CCNGG 5 cut(s) 410, 808, 1307, 1421, 1538
MunI CAATTG 2 cut(s) 1398, 1467
Mva1269I GAATGC 1 cut(s) 1580
MvaI CCWGG 4 cut(s) 410, 1307, 1421, 1538
MvnI CGCG 2 cut(s) 563, 1661
MwoI GCNNNNNNNGC 7 cut(s) 204, 213, 547, 1040, 1200, 1401, 1648
NciI CCSGG 1 cut(s) 808
NcoI CCATGG 3 cut(s) 1473, 1745, 1804
NdeI CATATG 1 cut(s) 771
NdeII GATC 6 cut(s) 145, 577, 700, 708, 751, 996
NlaIV GGNNCC 4 cut(s) 122, 270, 777, 1565
NmuCI GTSAC 3 cut(s) 977, 1354, 1522
NsbI TGCGCA 1 cut(s) 171
NspI RCATGY 2 cut(s) 421, 913
PaeI GCATGC 1 cut(s) 421
PceI AGGCCT 2 cut(s) 207, 1651
PctI GAATGC 1 cut(s) 1580
PdmI GAANNNNTTC 2 cut(s) 780, 1562
PfeI GAWTC 9 cut(s) 43, 65, 460, 479, 565, 1211, 1258, 1529, 1628
PkrI GCNGC 6 cut(s) 200, 302, 669, 932, 1152, 1552
PleI GAGTC 4 cut(s) 377, 451, 948, 1306
PpsI GAGTC 4 cut(s) 377, 451, 948, 1306
PshBI ATTAAT 1 cut(s) 32
PsiI TTATAA 1 cut(s) 1130
Psp124BI GAGCTC 2 cut(s) 622, 1720
Psp6I CCWGG 4 cut(s) 408, 1305, 1419, 1536
PspEI GGTNACC 1 cut(s) 1354
PspGI CCWGG 4 cut(s) 408, 1305, 1419, 1536
PspN4I GGNNCC 4 cut(s) 122, 270, 777, 1565
PspOMI GGGCCC 1 cut(s) 120
PspPI GGNCC 4 cut(s) 120, 121, 269, 1597
PstNI CAGNNNCTG 2 cut(s) 1271, 1344
PsuI RGATCY 1 cut(s) 751
PvuII CAGCTG 2 cut(s) 706, 1153
RsaI GTAC 4 cut(s) 520, 858, 1348, 1483
RsaNI GTAC 4 cut(s) 519, 857, 1347, 1482
SacI GAGCTC 2 cut(s) 622, 1720
SatI GCNGC 6 cut(s) 199, 301, 668, 931, 1151, 1551
Sau3AI GATC 6 cut(s) 145, 577, 700, 708, 751, 996
Sau96I GGNCC 4 cut(s) 120, 121, 269, 1597
ScaI AGTACT 1 cut(s) 520
SchI GAGTC 4 cut(s) 377, 452, 948, 1306
ScrFI CCNGG 5 cut(s) 410, 808, 1307, 1421, 1538
SduI GDGCHC 3 cut(s) 124, 622, 1720
SfaNI GCATC 3 cut(s) 157, 419, 833
SfcI CTRYAG 1 cut(s) 941
SinI GGWCC 1 cut(s) 1597
SmlI CTYRAG 4 cut(s) 50, 398, 455, 551
SmoI CTYRAG 4 cut(s) 50, 398, 455, 551
SphI GCATGC 1 cut(s) 421
Sse9I AATT 9 cut(s) 244, 289, 374, 879, 1025, 1109, 1398, 1467, 1799
SseBI AGGCCT 2 cut(s) 207, 1651
SsiI CCGC 4 cut(s) 153, 235, 724, 1661
SspMI CTAG 5 cut(s) 371, 516, 572, 749, 1701
SstI GAGCTC 2 cut(s) 622, 1720
StuI AGGCCT 2 cut(s) 207, 1651
StyD4I CCNGG 5 cut(s) 408, 806, 1305, 1419, 1536
StyI CCWWGG 4 cut(s) 1170, 1473, 1745, 1804
TaaI ACNGT 3 cut(s) 223, 945, 1346
TaqI TCGA 9 cut(s) 63, 144, 240, 463, 477, 792, 995, 1036, 1383
TaqII GACCGA 1 cut(s) 1373
TasI AATT 9 cut(s) 244, 289, 374, 879, 1025, 1109, 1398, 1467, 1799
TatI WGTACW 2 cut(s) 518, 1346
TfiI GAWTC 9 cut(s) 43, 65, 460, 479, 565, 1211, 1258, 1529, 1628
TscAI CASTG 6 cut(s) 954, 982, 1141, 1285, 1618, 1676
TseFI GTSAC 3 cut(s) 977, 1354, 1522
TseI GCWGC 6 cut(s) 198, 300, 667, 930, 1150, 1550
Tsp45I GTSAC 3 cut(s) 977, 1354, 1522
TspDTI ATGAA 4 cut(s) 116, 548, 873, 1620
TspGWI ACGGA 2 cut(s) 1499, 1728
TspRI CASTG 6 cut(s) 954, 982, 1141, 1285, 1618, 1676
Vha464I CTTAAG 2 cut(s) 398, 455
VpaK11BI GGWCC 1 cut(s) 1597
VspI ATTAAT 1 cut(s) 32
XagI CCTNNNNNAGG 1 cut(s) 1100
XapI RAATTY 4 cut(s) 244, 289, 879, 1025
XceI RCATGY 2 cut(s) 421, 913
XmiI GTMKAC 2 cut(s) 381, 940
XmnI GAANNNNTTC 2 cut(s) 780, 1562
XspI CTAG 5 cut(s) 371, 516, 572, 749, 1701
ZrmI AGTACT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.