Rw5G045260

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008578
Physical Location & Seq
Reverse (-)
79128116 .. 79130077
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045260.1

Sequence Viewer

Length: 1407 bp
ATGAACCTGGACAGAATGCTTAATGGTTGTTTATGCTTGGAGTCTTTATCTATAAAGATTCGATGGAGAGGTGTAGGTGCAAATCCTTTACAGCCATTTCAATTCTTCCTGCCTAAAACTCTCAAAAGCATCAAGTTGCAGCCAGTGCTTGAGGAAGACGTAATCCATTTCACTAGTTTGAATGATGCATTTAGGAACTATTTGCCAACAGCTAATGTCAGCACCCCTGTTTATAAATCCTCATTTGCATTCGCATTGCAGCGCTTGTCGCTTGTCTTAGATGTTATATCAGATCAGCTAATTGTCGCCATTGGGATTTGTGACAATTTGGTAGAGCTGGATCTTGAAGATAGACCAATTCAGGAACCATTGCCAAACCATGACTTAACCAGCGTGGGGCTTATGAGTCTGGCCTCTAATTTTCGTTTGAAGGGATTGTCTCTTGTAAGAAGTAGACAGAACTGCCAAGTCTCATTTAAGGGAGTGGATGATATGGGCATGTTTGTTTTTTCTAAGTACTGCACGACTCTAGAGTCGGTGAGACTTTTTGGTTTCTCCAGAGTTAGTGATGCAGGCTATGCATCCGTCCTACAATCGTGTCAGAATTTGAAGAAATTCGAAGTGCGTAATGCCTTCCTTATGTCAGACTTGGCCTTTGTTGACCTTACTCGGTTCTCATGTTCTCTTGTTGAGGTGAAATTGTTGTCATGCTGTCTTATAACTTGTGAAACGGTAAATCAGCTCACCAGTTCTGGGGTCCTGGAGGTGCTTGATCTGTATGGCTGTAGGAGCATAGCAGACTCCTGCCTCATCTCCATTGCATCGCTTCATAGCTTAACCATGCTGAATCTTTCAGGAGCTGATATTTCTGATTACGGCTTATCTGTTCTTGGGACAGGGAATCCACCCATCACACATTTGTGTCTTAGAAACTGTAAGGGTGTGACTGATGAAGGAGTTTTACTTTTGTTTCTTGGAGGAGGCACAATTGGCAAAACACTGTCATCCCTCGATTTAGGACACATACCAGGAATATCTGATAAAGCAGTTCTCACAATTGCTGTGGCCGGTACAGGGATTACTGAGTTGTGTCTCAGTTATTGCTCCTCTATGACTAATTCGTCCCTAGAAGCTTTGGCTACAAGGAAACCGGATCAAGATGATTGTAAACTACTTCGGAGGCTTGATCTTTTCAATTGCAAGGGTTTGTCTGCTGACTCAATTAGGTTGCTGGAGAGGCCTTTCTTCCCCGGACTGCACTGGCTTGGTATTGGGAATACTCGTTTGAATACTAAAGGATGTCCAGCTCTCAGTGAAATTGGTTACGAAAGACCATGGCTGACGATATGTTTGGATGGCTGTGAAATGGGTTGTCATGATGGATGGCAATTCCATAGAGTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

468

Amino Acids

51.31

Weight (kDa)

5.94

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 2 - 460 2e-153 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 234, 719
AasI GACNNNNNNGTC 2 cut(s) 532, 1120
AccI GTMKAC 1 cut(s) 454
AclWI GGATC 2 cut(s) 348, 1161
AcoI YGGCCR 1 cut(s) 1065
AcsI RAATTY 2 cut(s) 604, 614
AfaI GTAC 2 cut(s) 518, 1072
AfeI AGCGCT 1 cut(s) 263
AfiI CCNNNNNNNGG 3 cut(s) 396, 753, 1074
AgsI TTSAA 7 cut(s) 101, 181, 347, 430, 610, 1195, 1288
AhlI ACTAGT 1 cut(s) 173
AjnI CCWGG 3 cut(s) 6, 759, 1027
AleI CACNNNNGTG 1 cut(s) 919
AluBI AGCT 8 cut(s) 212, 298, 337, 742, 834, 860, 1133, 1307
AluI AGCT 8 cut(s) 212, 298, 337, 742, 834, 860, 1133, 1307
Alw26I GTCTC 4 cut(s) 444, 475, 535, 1097
AlwI GGATC 2 cut(s) 348, 1161
AlwNI CAGNNNCTG 1 cut(s) 860
Aor51HI AGCGCT 1 cut(s) 263
AoxI GGCC 4 cut(s) 411, 651, 1065, 1238
ApeKI GCWGC 2 cut(s) 139, 259
ApoI RAATTY 2 cut(s) 604, 614
ArsI GACNNNNNNTTYG 4 cut(s) 638, 670, 1333, 1365
Asp700I GAANNNNTTC 1 cut(s) 614
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 757
AsuC2I CCSGG 1 cut(s) 1251
AsuHPI GGTGA 3 cut(s) 550, 706, 736
AsuII TTCGAA 1 cut(s) 618
AvaII GGWCC 1 cut(s) 757
BarI GAAGNNNNNNTAC 2 cut(s) 945, 977
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 151, 271
BccI CCATC 5 cut(s) 57, 917, 1349, 1373, 1377
BceAI ACGGC 1 cut(s) 892
BciT130I CCWGG 3 cut(s) 8, 761, 1029
BcnI CCSGG 1 cut(s) 1251
BcoDI GTCTC 4 cut(s) 444, 475, 535, 1097
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 3 cut(s) 174, 530, 1127
BfmI CTRYAG 1 cut(s) 784
BfoI RGCGCY 1 cut(s) 265
BisI GCNGC 2 cut(s) 140, 260
BlsI GCNGC 2 cut(s) 141, 261
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 4 cut(s) 8, 761, 1029, 1251
Bme18I GGWCC 1 cut(s) 757
BmgT120I GGNCC 1 cut(s) 757
BmiI GGNNCC 2 cut(s) 366, 758
BmrFI CCNGG 4 cut(s) 8, 761, 1029, 1251
BmsI GCATC 5 cut(s) 138, 175, 559, 590, 830
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 3 cut(s) 541, 782, 1253
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 1251
BsaJI CCNNGG 2 cut(s) 1249, 1334
BsaWI WCCGGW 1 cut(s) 1150
Bsc4I CCNNNNNNNGG 3 cut(s) 396, 753, 1074
Bse118I RCCGGY 1 cut(s) 1067
Bse1I ACTGG 3 cut(s) 143, 747, 1265
Bse3DI GCAATG 3 cut(s) 254, 368, 816
BseBI CCWGG 3 cut(s) 8, 761, 1029
BseDI CCNNGG 2 cut(s) 1249, 1334
BseGI GGATG 6 cut(s) 493, 581, 1004, 1304, 1360, 1388
BseLI CCNNNNNNNGG 3 cut(s) 396, 753, 1074
BseMI GCAATG 3 cut(s) 254, 368, 816
BseMII CTCAG 3 cut(s) 1074, 1108, 1324
BseNI ACTGG 3 cut(s) 143, 747, 1265
BseRI GAGGAG 2 cut(s) 993, 1096
BseXI GCAGC 2 cut(s) 151, 271
BsgI GTGCAG 2 cut(s) 505, 1241
BshFI GGCC 4 cut(s) 413, 653, 1067, 1240
BsiSI CCGG 3 cut(s) 1068, 1151, 1251
BslFI GGGAC 2 cut(s) 907, 1108
BslI CCNNNNNNNGG 3 cut(s) 396, 753, 1074
BsmAI GTCTC 4 cut(s) 444, 475, 535, 1097
BsmFI GGGAC 2 cut(s) 907, 1108
BsmI GAATGC 2 cut(s) 21, 248
BsnI GGCC 4 cut(s) 413, 653, 1067, 1240
Bsp119I TTCGAA 1 cut(s) 618
Bsp143I GATC 5 cut(s) 292, 340, 772, 1153, 1186
Bsp19I CCATGG 1 cut(s) 1334
BspANI GGCC 4 cut(s) 413, 653, 1067, 1240
BspCNI CTCAG 3 cut(s) 1075, 1107, 1323
BspHI TCATGA 1 cut(s) 1375
BspLI GGNNCC 2 cut(s) 366, 758
BspPI GGATC 2 cut(s) 348, 1161
BspT104I TTCGAA 1 cut(s) 618
BsrDI GCAATG 3 cut(s) 254, 368, 816
BsrFI RCCGGY 1 cut(s) 1067
BsrI ACTGG 3 cut(s) 143, 747, 1265
BssAI RCCGGY 1 cut(s) 1067
BssECI CCNNGG 2 cut(s) 1249, 1334
BssMI GATC 5 cut(s) 292, 340, 772, 1153, 1186
BssT1I CCWWGG 1 cut(s) 1334
Bst2UI CCWGG 3 cut(s) 8, 761, 1029
Bst4CI ACNGT 3 cut(s) 733, 935, 1002
BstAPI GCANNNNNTGC 2 cut(s) 145, 578
BstBI TTCGAA 1 cut(s) 618
BstC8I GCNNGC 1 cut(s) 574
BstDEI CTNAG 6 cut(s) 277, 513, 926, 1083, 1094, 1310
BstDSI CCRYGG 1 cut(s) 1334
BstF5I GGATG 6 cut(s) 493, 581, 1004, 1304, 1360, 1388
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 5 cut(s) 295, 343, 775, 1156, 1189
BstMAI GTCTC 4 cut(s) 444, 475, 535, 1097
BstMBI GATC 5 cut(s) 292, 340, 772, 1153, 1186
BstMWI GCNNNNNNNGC 6 cut(s) 145, 268, 578, 789, 990, 1237
BstNI CCWGG 3 cut(s) 8, 761, 1029
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 4 cut(s) 6, 759, 1027, 1249
BstSFI CTRYAG 1 cut(s) 784
BstV1I GCAGC 2 cut(s) 151, 271
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuRI GGCC 4 cut(s) 413, 653, 1067, 1240
BtgI CCRYGG 1 cut(s) 1334
BtgZI GCGATG 1 cut(s) 807
BtsCI GGATG 6 cut(s) 493, 581, 1004, 1304, 1360, 1388
BtsIMutI CAGTG 4 cut(s) 150, 998, 1258, 1318
Cac8I GCNNGC 1 cut(s) 574
CaiI CAGNNNCTG 1 cut(s) 860
CciI TCATGA 1 cut(s) 1375
CfoI GCGC 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 1 cut(s) 757
Csp6I GTAC 2 cut(s) 517, 1071
CspCI CAANNNNNGTGG 2 cut(s) 1044, 1079
CviAII CATG 7 cut(s) 380, 499, 678, 708, 841, 1335, 1376
CviQI GTAC 2 cut(s) 517, 1071
DdeI CTNAG 6 cut(s) 277, 513, 926, 1083, 1094, 1310
DpnI GATC 5 cut(s) 294, 342, 774, 1155, 1188
DpnII GATC 5 cut(s) 292, 340, 772, 1153, 1186
DrdI GACNNNNNNGTC 2 cut(s) 532, 1120
DseDI GACNNNNNNGTC 2 cut(s) 532, 1120
EaeI YGGCCR 1 cut(s) 1065
Eco130I CCWWGG 1 cut(s) 1334
Eco147I AGGCCT 1 cut(s) 1240
Eco47I GGWCC 1 cut(s) 757
Eco47III AGCGCT 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 757
EcoRII CCWGG 3 cut(s) 6, 759, 1027
EcoT14I CCWWGG 1 cut(s) 1334
EcoT22I ATGCAT 2 cut(s) 190, 583
ErhI CCWWGG 1 cut(s) 1334
FaeI CATG 7 cut(s) 383, 502, 681, 711, 844, 1338, 1379
FaqI GGGAC 2 cut(s) 907, 1108
FatI CATG 7 cut(s) 379, 498, 677, 707, 840, 1334, 1375
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 2 cut(s) 140, 260
FokI GGATG 6 cut(s) 500, 568, 991, 1311, 1367, 1395
Fsp4HI GCNGC 2 cut(s) 140, 260
FspBI CTAG 3 cut(s) 174, 530, 1127
GlaI GCGC 1 cut(s) 263
GluI GCNGC 2 cut(s) 140, 260
GsuI CTGGAG 3 cut(s) 541, 782, 1253
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 4 cut(s) 413, 653, 1067, 1240
HapII CCGG 3 cut(s) 1068, 1151, 1251
HhaI GCGC 1 cut(s) 264
Hin1II CATG 7 cut(s) 383, 502, 681, 711, 844, 1338, 1379
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HindIII AAGCTT 1 cut(s) 1131
HinfI GANTC 9 cut(s) 41, 58, 406, 526, 533, 800, 847, 901, 1217
HpaII CCGG 3 cut(s) 1068, 1151, 1251
HphI GGTGA 3 cut(s) 550, 706, 736
Hpy166II GTNNAC 3 cut(s) 455, 661, 1169
Hpy188I TCNGA 6 cut(s) 292, 603, 646, 871, 1039, 1179
Hpy188III TCNNGA 7 cut(s) 344, 362, 530, 558, 855, 1157, 1376
Hpy8I GTNNAC 3 cut(s) 455, 661, 1169
HpyAV CCTTC 3 cut(s) 424, 643, 947
HpyCH4III ACNGT 3 cut(s) 733, 935, 1002
HpyCH4IV ACGT 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 6 cut(s) 145, 268, 578, 789, 990, 1237
HpyF3I CTNAG 6 cut(s) 277, 513, 926, 1083, 1094, 1310
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 7 cut(s) 383, 502, 681, 711, 844, 1338, 1379
HspAI GCGC 1 cut(s) 262
Kzo9I GATC 5 cut(s) 292, 340, 772, 1153, 1186
LmnI GCTCC 3 cut(s) 789, 857, 1109
Lsp1109I GCAGC 2 cut(s) 151, 271
LweI GCATC 5 cut(s) 138, 175, 559, 590, 830
MaeI CTAG 3 cut(s) 174, 530, 1127
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 3 cut(s) 320, 943, 1322
MalI GATC 5 cut(s) 294, 342, 774, 1155, 1188
MboI GATC 5 cut(s) 292, 340, 772, 1153, 1186
MboII GAAGA 5 cut(s) 97, 167, 359, 622, 1237
MfeI CAATTG 3 cut(s) 987, 1056, 1195
MflI RGATCY 1 cut(s) 340
MlyI GAGTC 6 cut(s) 50, 415, 520, 542, 794, 1211
Mph1103I ATGCAT 2 cut(s) 190, 583
MroXI GAANNNNTTC 1 cut(s) 614
MseI TTAA 4 cut(s) 21, 386, 477, 836
MslI CAYNNNNRTG 1 cut(s) 919
MspI CCGG 3 cut(s) 1068, 1151, 1251
MspR9I CCNGG 4 cut(s) 8, 761, 1029, 1251
MunI CAATTG 3 cut(s) 987, 1056, 1195
Mva1269I GAATGC 2 cut(s) 21, 248
MvaI CCWGG 3 cut(s) 8, 761, 1029
MwoI GCNNNNNNNGC 6 cut(s) 145, 268, 578, 789, 990, 1237
NciI CCSGG 1 cut(s) 1251
NcoI CCATGG 1 cut(s) 1334
NdeII GATC 5 cut(s) 292, 340, 772, 1153, 1186
NlaIII CATG 7 cut(s) 383, 502, 681, 711, 844, 1338, 1379
NlaIV GGNNCC 2 cut(s) 366, 758
NmuCI GTSAC 2 cut(s) 320, 943
NsiI ATGCAT 2 cut(s) 190, 583
NspI RCATGY 1 cut(s) 502
NspV TTCGAA 1 cut(s) 618
OliI CACNNNNGTG 1 cut(s) 919
PagI TCATGA 1 cut(s) 1375
PceI AGGCCT 1 cut(s) 1240
PctI GAATGC 2 cut(s) 21, 248
PdmI GAANNNNTTC 1 cut(s) 614
PfeI GAWTC 3 cut(s) 58, 847, 901
PfoI TCCNGGA 1 cut(s) 759
PkrI GCNGC 2 cut(s) 141, 261
PleI GAGTC 6 cut(s) 49, 414, 520, 541, 794, 1211
PpsI GAGTC 6 cut(s) 49, 414, 520, 541, 794, 1211
PpuMI RGGWCCY 1 cut(s) 757
PsiI TTATAA 2 cut(s) 234, 719
Psp5II RGGWCCY 1 cut(s) 757
Psp6I CCWGG 3 cut(s) 6, 759, 1027
PspGI CCWGG 3 cut(s) 6, 759, 1027
PspN4I GGNNCC 2 cut(s) 366, 758
PspPI GGNCC 1 cut(s) 757
PspPPI RGGWCCY 1 cut(s) 757
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 2 cut(s) 518, 1072
RsaNI GTAC 2 cut(s) 517, 1071
RseI CAYNNNNRTG 1 cut(s) 919
SaqAI TTAA 4 cut(s) 21, 386, 477, 836
SatI GCNGC 2 cut(s) 140, 260
Sau3AI GATC 5 cut(s) 292, 340, 772, 1153, 1186
Sau96I GGNCC 1 cut(s) 757
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 6 cut(s) 50, 415, 520, 542, 794, 1211
ScrFI CCNGG 4 cut(s) 8, 761, 1029, 1251
SfaNI GCATC 5 cut(s) 138, 175, 559, 590, 830
SfcI CTRYAG 1 cut(s) 784
SfuI TTCGAA 1 cut(s) 618
SinI GGWCC 1 cut(s) 757
SmiMI CAYNNNNRTG 1 cut(s) 919
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SpeI ACTAGT 1 cut(s) 173
SseBI AGGCCT 1 cut(s) 1240
SspMI CTAG 3 cut(s) 174, 530, 1127
StuI AGGCCT 1 cut(s) 1240
StyD4I CCNGG 4 cut(s) 6, 759, 1027, 1249
StyI CCWWGG 1 cut(s) 1334
TaaI ACNGT 3 cut(s) 733, 935, 1002
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 61, 618, 1011
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 3 cut(s) 58, 847, 901
Tru1I TTAA 4 cut(s) 21, 386, 477, 836
Tru9I TTAA 4 cut(s) 21, 386, 477, 836
TscAI CASTG 4 cut(s) 150, 1005, 1265, 1318
TseFI GTSAC 2 cut(s) 320, 943
TseI GCWGC 2 cut(s) 139, 259
Tsp45I GTSAC 2 cut(s) 320, 943
TspDTI ATGAA 3 cut(s) 17, 818, 966
TspGWI ACGGA 1 cut(s) 574
TspRI CASTG 4 cut(s) 150, 1005, 1265, 1318
VpaK11BI GGWCC 1 cut(s) 757
XapI RAATTY 2 cut(s) 604, 614
XbaI TCTAGA 1 cut(s) 529
XceI RCATGY 1 cut(s) 502
XmiI GTMKAC 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 614
XspI CTAG 3 cut(s) 174, 530, 1127
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 2 cut(s) 190, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.