Rorug05G0430000

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008578
Physical Location & Seq
Forward (+)
59784221 .. 59785973
1753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0430000.1

Sequence Viewer

Length: 1149 bp
ATGGCAGAGGACGGCGGCGGCCGTAAGCGAGCTCACACCGAGTTCCACGACGGCGACATAATTTCAGAGATTCTGGCGAGGCTACCGGTCAAATCTCTGATGCGATTCCGGTGCGTATCCAAGTCGTGGAGTGCTCTGATCTCCGATCCTTACTTCACAAAGAAGCACTTAAGCTACACAGAAATGGGCATCACTGAGAACAGCTCCAGGCTCCTTTATTCACTGGACCCTCCACAATCCCTAGACCACGAAGCATTGAAGGATTTGAAGGATCATAGGGATGGTTATTTTGCCAACAGAGAGCTTGATCTGCCGGTAATGATCCCGGGCTCATCCAATCGTTATAAAAGGATCGTTGGTTGTTGCAATGGGTTAATATGTATTTTAGTTGAACAAGTCGACTTTATGTTATGGAATCCATGTACTAGATACTCCAACGTGTTACCAAGAACAAAATTCCCTTCCTATTTTCCAAAATTTAATAGTCGGAACGTGTTTTGTGGGTTCGGTTATGATTCTGCTACCGATGATTACAAGGTAATAATGGGGACTAGAAACTGCAAACAAATTCAGATTTTCACTCTAAAAACGAGTTCATGGAAGGTGCTCCCCTTAGCCAATGCTCTTGACATGTCAAGTCGGGCGGGGTTGTTATTGAATGGATCTTTGCACTGGTTGGCTGAACCCCAGCCAAATGCAGGGAATCGCCAAACAATATTGTCTTTTGATTTGGTGGAGGACAAGTTTCGTGAGATGATTCCATTACCCCATCATGATGCTTACTTTCAGGGTATGACGATTCTTAGAAATTATCTTTGTGTGTTTGAACTCTTAGTTGGCCGGGATCGTGTTAACACAATATGGTTGATGAAGGAGTATGGGGTGAAGCAATCTTGGACTCAAGTTATAAGGTGTTCTAAAAAGGTTATGCCTCAAGAGTACAGACATGTGTTCATGCAGCCTTTTTGTATTCTAGAGAATGGAGAACTTTTGGTTAATGATTGGCGTGCAGGTTTCTTGGTGTTATATGATCCAAAGGAAAATACATTCAAGAATGTTACCAAGACTCATGATCAATTGCGTTGTGGAGCTTTAGTTTACAGAGAGACTTTAGTTTCACCAGTAACCGGCAGTGTTGTAGACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

382

Amino Acids

43.97

Weight (kDa)

8.66

Isoelectric Point (pI)

34.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 19 - 54 1.6e-09 F-box domain
FBA_3 PF08268 104 - 354 3.9e-27 F-box associated beta propeller domain
FBA_1 PF07734 119 - 333 2e-26 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 345, 908
Acc36I ACCTGC 1 cut(s) 1001
AccB7I CCANNNNNTGG 1 cut(s) 126
AccI GTMKAC 2 cut(s) 399, 1140
AciI CCGC 3 cut(s) 15, 18, 644
AclWI GGATC 7 cut(s) 140, 279, 316, 359, 670, 852, 1025
AcoI YGGCCR 2 cut(s) 19, 838
AcsI RAATTY 3 cut(s) 455, 476, 567
AfaI GTAC 2 cut(s) 424, 941
AfiI CCNNNNNNNGG 3 cut(s) 126, 698, 1127
AflII CTTAAG 1 cut(s) 169
AflIII ACRYGT 4 cut(s) 438, 492, 630, 946
AgeI ACCGGT 1 cut(s) 85
AgsI TTSAA 6 cut(s) 259, 268, 392, 658, 827, 1051
AjnI CCWGG 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 819, 851
AluBI AGCT 5 cut(s) 32, 174, 204, 304, 1091
AluI AGCT 5 cut(s) 32, 174, 204, 304, 1091
Alw21I GWGCWC 3 cut(s) 34, 136, 609
Alw26I GTCTC 1 cut(s) 1100
AlwI GGATC 7 cut(s) 140, 279, 316, 359, 670, 852, 1025
Ama87I CYCGRG 1 cut(s) 325
AoxI GGCC 2 cut(s) 19, 838
ApeKI GCWGC 1 cut(s) 958
ApoI RAATTY 3 cut(s) 455, 476, 567
AsiGI ACCGGT 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 3 cut(s) 326, 327, 842
AsuHPI GGTGA 2 cut(s) 895, 1110
AvaI CYCGRG 1 cut(s) 325
AvaII GGWCC 1 cut(s) 226
BanII GRGCYC 2 cut(s) 34, 332
Bbv12I GWGCWC 3 cut(s) 34, 136, 609
BbvI GCAGC 1 cut(s) 970
BccI CCATC 2 cut(s) 275, 777
BceAI ACGGC 3 cut(s) 6, 28, 67
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BciT130I CCWGG 1 cut(s) 208
BciVI GTATCC 1 cut(s) 127
BclI TGATCA 1 cut(s) 1072
BcnI CCSGG 3 cut(s) 326, 327, 842
BcoDI GTCTC 1 cut(s) 1100
BfaI CTAG 4 cut(s) 242, 426, 552, 974
BfrI CTTAAG 1 cut(s) 169
BfuAI ACCTGC 1 cut(s) 1001
BfuI GTATCC 1 cut(s) 127
BisI GCNGC 3 cut(s) 16, 19, 959
BlsI GCNGC 3 cut(s) 17, 20, 960
Bme1390I CCNGG 4 cut(s) 208, 326, 327, 842
Bme18I GGWCC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 2 cut(s) 212, 228
BmrFI CCNGG 4 cut(s) 208, 326, 327, 842
BmsI GCATC 3 cut(s) 90, 198, 766
BplI GAGNNNNNCTC 2 cut(s) 188, 220
BpmI CTGGAG 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 613
BpuEI CTTGAG 2 cut(s) 885, 918
BpuMI CCSGG 3 cut(s) 326, 327, 842
BsaJI CCNNGG 1 cut(s) 325
BsaWI WCCGGW 2 cut(s) 85, 108
BsaXI ACNNNNNCTCC 4 cut(s) 728, 758, 1080, 1110
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 698, 1127
Bse118I RCCGGY 3 cut(s) 85, 313, 1127
Bse1I ACTGG 3 cut(s) 228, 677, 1121
Bse3DI GCAATG 1 cut(s) 373
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 2 cut(s) 286, 332
BseLI CCNNNNNNNGG 3 cut(s) 126, 698, 1127
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 3 cut(s) 228, 677, 1121
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 1 cut(s) 970
BseYI CCCAGC 1 cut(s) 687
BsgI GTGCAG 1 cut(s) 1029
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 21, 840
BshTI ACCGGT 1 cut(s) 85
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 3 cut(s) 34, 136, 609
BsiHKCI CYCGRG 1 cut(s) 325
BsiSI CCGG 6 cut(s) 86, 109, 314, 326, 841, 1128
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 3 cut(s) 126, 698, 1127
BsmAI GTCTC 1 cut(s) 1100
BsmFI GGGAC 1 cut(s) 562
BsnI GGCC 2 cut(s) 21, 840
BsoBI CYCGRG 1 cut(s) 325
Bsp1286I GDGCHC 4 cut(s) 34, 136, 332, 609
BspACI CCGC 3 cut(s) 15, 18, 644
BspANI GGCC 2 cut(s) 21, 840
BspCNI CTCAG 1 cut(s) 187
BspHI TCATGA 2 cut(s) 772, 1069
BspLI GGNNCC 2 cut(s) 212, 228
BspMI ACCTGC 1 cut(s) 1001
BspPI GGATC 7 cut(s) 140, 279, 316, 359, 670, 852, 1025
BspTI CTTAAG 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 3 cut(s) 85, 313, 1127
BsrI ACTGG 3 cut(s) 228, 677, 1121
BssAI RCCGGY 3 cut(s) 85, 313, 1127
BssECI CCNNGG 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 208
BstAFI CTTAAG 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 30, 1008
BstDEI CTNAG 4 cut(s) 195, 613, 803, 832
BstF5I GGATG 2 cut(s) 286, 332
BstMAI GTCTC 1 cut(s) 1100
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 2 cut(s) 634, 950
BstSCI CCNGG 4 cut(s) 206, 324, 325, 840
BstV1I GCAGC 1 cut(s) 970
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BstZI CGGCCG 1 cut(s) 19
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 2 cut(s) 21, 840
BtsCI GGATG 2 cut(s) 286, 332
BtsI GCAGTG 1 cut(s) 1138
BtsIMutI CAGTG 4 cut(s) 192, 221, 670, 1138
BveI ACCTGC 1 cut(s) 1001
Cac8I GCNNGC 2 cut(s) 30, 1008
CciI TCATGA 2 cut(s) 772, 1069
Cfr10I RCCGGY 3 cut(s) 85, 313, 1127
Cfr13I GGNCC 1 cut(s) 226
Cfr9I CCCGGG 1 cut(s) 325
Csp6I GTAC 2 cut(s) 423, 940
CspAI ACCGGT 1 cut(s) 85
CviAII CATG 7 cut(s) 420, 597, 631, 773, 947, 955, 1070
CviQI GTAC 2 cut(s) 423, 940
DdeI CTNAG 4 cut(s) 195, 613, 803, 832
EaeI YGGCCR 2 cut(s) 19, 838
EagI CGGCCG 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 32
EclXI CGGCCG 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 34, 332
Eco47I GGWCC 1 cut(s) 226
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 206
EcoT38I GRGCYC 2 cut(s) 34, 332
FaeI CATG 7 cut(s) 423, 600, 634, 776, 950, 958, 1073
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 1 cut(s) 562
FatI CATG 7 cut(s) 419, 596, 630, 772, 946, 954, 1069
FauI CCCGC 1 cut(s) 637
FbaI TGATCA 1 cut(s) 1072
FblI GTMKAC 2 cut(s) 399, 1140
Fnu4HI GCNGC 3 cut(s) 16, 19, 959
FokI GGATG 2 cut(s) 293, 319
FriOI GRGCYC 2 cut(s) 34, 332
Fsp4HI GCNGC 3 cut(s) 16, 19, 959
FspBI CTAG 4 cut(s) 242, 426, 552, 974
GluI GCNGC 3 cut(s) 16, 19, 959
GsaI CCCAGC 1 cut(s) 691
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 2 cut(s) 21, 840
HapII CCGG 6 cut(s) 86, 109, 314, 326, 841, 1128
Hin1II CATG 7 cut(s) 423, 600, 634, 776, 950, 958, 1073
HincII GTYRAC 2 cut(s) 400, 853
HindII GTYRAC 2 cut(s) 400, 853
HinfI GANTC 9 cut(s) 70, 105, 415, 515, 703, 757, 799, 898, 1066
HpaI GTTAAC 1 cut(s) 853
HpaII CCGG 6 cut(s) 86, 109, 314, 326, 841, 1128
HphI GGTGA 2 cut(s) 895, 1110
Hpy166II GTNNAC 4 cut(s) 400, 853, 1099, 1141
Hpy188I TCNGA 7 cut(s) 67, 99, 138, 145, 489, 573, 1148
Hpy188III TCNNGA 7 cut(s) 626, 749, 773, 935, 974, 1051, 1070
Hpy8I GTNNAC 4 cut(s) 400, 853, 1099, 1141
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 5 cut(s) 253, 262, 471, 595, 865
HpyCH4IV ACGT 2 cut(s) 438, 492
HpyCH4V TGCA 6 cut(s) 366, 561, 670, 698, 958, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 4 cut(s) 195, 613, 803, 832
HpySE526I ACGT 2 cut(s) 438, 492
Hsp92II CATG 7 cut(s) 423, 600, 634, 776, 950, 958, 1073
Ksp22I TGATCA 1 cut(s) 1072
KspAI GTTAAC 1 cut(s) 853
LmnI GCTCC 4 cut(s) 209, 216, 612, 1088
Lsp1109I GCAGC 1 cut(s) 970
LweI GCATC 3 cut(s) 90, 198, 766
MaeI CTAG 4 cut(s) 242, 426, 552, 974
MaeII ACGT 2 cut(s) 438, 492
MaeIII GTNAC 3 cut(s) 441, 1057, 1123
MfeI CAATTG 1 cut(s) 1076
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 4 cut(s) 34, 136, 332, 609
MluCI AATT 6 cut(s) 60, 455, 476, 567, 808, 1076
MlyI GAGTC 2 cut(s) 892, 1060
MmeI TCCRAC 2 cut(s) 459, 467
MnlI CCTC 4 cut(s) 72, 240, 730, 942
MseI TTAA 5 cut(s) 170, 374, 480, 852, 996
MslI CAYNNNNRTG 3 cut(s) 182, 279, 774
MspCI CTTAAG 1 cut(s) 169
MspI CCGG 6 cut(s) 86, 109, 314, 326, 841, 1128
MspR9I CCNGG 4 cut(s) 208, 326, 327, 842
MunI CAATTG 1 cut(s) 1076
MvaI CCWGG 1 cut(s) 208
MwoI GCNNNNNNNGC 1 cut(s) 310
NciI CCSGG 3 cut(s) 326, 327, 842
NlaIII CATG 7 cut(s) 423, 600, 634, 776, 950, 958, 1073
NlaIV GGNNCC 2 cut(s) 212, 228
NspI RCATGY 2 cut(s) 634, 950
PagI TCATGA 2 cut(s) 772, 1069
PciI ACATGT 2 cut(s) 630, 946
PfeI GAWTC 7 cut(s) 70, 105, 415, 515, 703, 757, 799
PflMI CCANNNNNTGG 1 cut(s) 126
PinAI ACCGGT 1 cut(s) 85
PkrI GCNGC 3 cut(s) 17, 20, 960
PleI GAGTC 2 cut(s) 892, 1060
PpsI GAGTC 2 cut(s) 892, 1060
PscI ACATGT 2 cut(s) 630, 946
PsiI TTATAA 2 cut(s) 345, 908
Psp124BI GAGCTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 687
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 212, 228
PspPI GGNCC 1 cut(s) 226
PsuI RGATCY 1 cut(s) 662
RsaI GTAC 2 cut(s) 424, 941
RsaNI GTAC 2 cut(s) 423, 940
RseI CAYNNNNRTG 3 cut(s) 182, 279, 774
SacI GAGCTC 1 cut(s) 34
SalI GTCGAC 1 cut(s) 398
SaqAI TTAA 5 cut(s) 170, 374, 480, 852, 996
SatI GCNGC 3 cut(s) 16, 19, 959
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 2 cut(s) 892, 1060
ScrFI CCNGG 4 cut(s) 208, 326, 327, 842
SduI GDGCHC 4 cut(s) 34, 136, 332, 609
SfaNI GCATC 3 cut(s) 90, 198, 766
SinI GGWCC 1 cut(s) 226
SmaI CCCGGG 1 cut(s) 327
SmiMI CAYNNNNRTG 3 cut(s) 182, 279, 774
SmlI CTYRAG 3 cut(s) 169, 900, 933
SmoI CTYRAG 3 cut(s) 169, 900, 933
Sse9I AATT 6 cut(s) 60, 455, 476, 567, 808, 1076
SsiI CCGC 3 cut(s) 15, 18, 644
SspI AATATT 1 cut(s) 717
SspMI CTAG 4 cut(s) 242, 426, 552, 974
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 4 cut(s) 206, 324, 325, 840
TaiI ACGT 2 cut(s) 441, 495
TaqI TCGA 1 cut(s) 399
TasI AATT 6 cut(s) 60, 455, 476, 567, 808, 1076
TatI WGTACW 2 cut(s) 422, 939
TauI GCSGC 2 cut(s) 18, 21
TfiI GAWTC 7 cut(s) 70, 105, 415, 515, 703, 757, 799
Tru1I TTAA 5 cut(s) 170, 374, 480, 852, 996
Tru9I TTAA 5 cut(s) 170, 374, 480, 852, 996
TscAI CASTG 4 cut(s) 199, 228, 677, 1138
TseI GCWGC 1 cut(s) 958
TspDTI ATGAA 3 cut(s) 585, 884, 943
TspMI CCCGGG 1 cut(s) 325
TspRI CASTG 4 cut(s) 199, 228, 677, 1138
Van91I CCANNNNNTGG 1 cut(s) 126
Vha464I CTTAAG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 3 cut(s) 455, 476, 567
XbaI TCTAGA 1 cut(s) 973
XceI RCATGY 2 cut(s) 634, 950
XmaI CCCGGG 1 cut(s) 325
XmiI GTMKAC 2 cut(s) 399, 1140
XspI CTAG 4 cut(s) 242, 426, 552, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.