Rorug05G0429900

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008578
Physical Location & Seq
Reverse (-)
59773891 .. 59776464
2574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0429900.1

Sequence Viewer

Length: 528 bp
ATGGTTTATCACTCTAGTTTTGTAGATGACGAAGGAATTACTAAAGCTTGTGGGTTCCCTCTGCTGCCTTTGAAAAGCCACATAAAGGGTCCTGCTCCAGTTTCAGATCTAGATAGGACTGATATTGTTGATGAAGCAATTACATTCTTTCGCGCTAATGTCTTCTTTAGAAACTTTGATGTCAAGAGCTCGGCTGATAAGCTTCTCATTTATCTGACATTCTACATTAATGTGGCTTTGAAGAGGCTGGAAGGTTGCAGAACTTTGGCTGTAGGTACCAAAGCCATTATTAATCTAGGTCTCGAAAAAGTCCCTGTGCCTGGAGAGCCAGGTTTCCCTTTGCCAGGCCTTTTTCCTGTCCCTCAATCTGACGAGGAAGCAGAATTGCTCAGGAATTATTTGAGGCAGATAAGGGAGGAAGCAAGTGGGAGATTGTTGAGTGTTGCTTATAAATCGAATGGGACTCCTAATAAGTGGTGGTTGGCATTTGCCAAAAGGAAATTTATGAACATCATTCTTCCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

175

Amino Acids

19.6

Weight (kDa)

8.87

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
P21-Arc PF04062 2 - 171 1.9e-62 ARP2/3 complex ARPC3 (21 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 275
AccII CGCG 1 cut(s) 153
AcsI RAATTY 1 cut(s) 500
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 3 cut(s) 85, 320, 344
AgsI TTSAA 2 cut(s) 73, 241
AjnI CCWGG 3 cut(s) 319, 328, 343
AluBI AGCT 3 cut(s) 47, 189, 202
AluI AGCT 3 cut(s) 47, 189, 202
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 1 cut(s) 305
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 1 cut(s) 500
AseI ATTAAT 2 cut(s) 228, 291
Asp718I GGTACC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 89
AvaII GGWCC 1 cut(s) 89
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 51
BciT130I CCWGG 3 cut(s) 321, 330, 345
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 3 cut(s) 15, 110, 296
BfmI CTRYAG 1 cut(s) 270
BglII AGATCT 1 cut(s) 106
BisI GCNGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 66
Bme1390I CCNGG 3 cut(s) 321, 330, 345
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 3 cut(s) 56, 90, 277
BmrFI CCNGG 3 cut(s) 321, 330, 345
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 2 cut(s) 81, 342
Bpu10I CCTNAGC 1 cut(s) 389
BsaI GGTCTC 1 cut(s) 305
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 320, 344
Bse1I ACTGG 1 cut(s) 98
BseBI CCWGG 3 cut(s) 321, 330, 345
BseLI CCNNNNNNNGG 3 cut(s) 85, 320, 344
BseMII CTCAG 1 cut(s) 403
BseNI ACTGG 1 cut(s) 98
BseXI GCAGC 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 348
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 191
BslFI GGGAC 3 cut(s) 296, 344, 475
BslI CCNNNNNNNGG 3 cut(s) 85, 320, 344
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 3 cut(s) 296, 344, 475
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 1 cut(s) 106
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 402
BspFNI CGCG 1 cut(s) 153
BspHI TCATGA 1 cut(s) 524
BspLI GGNNCC 3 cut(s) 56, 90, 277
BspT107I GGYRCC 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 98
BssMI GATC 1 cut(s) 106
Bst2UI CCWGG 3 cut(s) 321, 330, 345
Bst6I CTCTTC 1 cut(s) 236
BstDEI CTNAG 1 cut(s) 389
BstENI CCTNNNNNAGG 1 cut(s) 342
BstFNI CGCG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstNI CCWGG 3 cut(s) 321, 330, 345
BstSCI CCNGG 3 cut(s) 319, 328, 343
BstSFI CTRYAG 1 cut(s) 270
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 51
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BsuRI GGCC 1 cut(s) 348
CciI TCATGA 1 cut(s) 524
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 1 cut(s) 276
CviAII CATG 1 cut(s) 525
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 1 cut(s) 389
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 189
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 89
EcoRII CCWGG 3 cut(s) 319, 328, 343
EcoT38I GRGCYC 1 cut(s) 191
FaeI CATG 1 cut(s) 528
FaiI YATR 4 cut(s) 83, 450, 506, 526
FaqI GGGAC 3 cut(s) 296, 344, 475
FatI CATG 1 cut(s) 524
Fnu4HI GCNGC 1 cut(s) 65
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 65
FspBI CTAG 3 cut(s) 15, 110, 296
GlaI GCGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 65
GsuI CTGGAG 2 cut(s) 81, 342
HaeIII GGCC 1 cut(s) 348
HhaI GCGC 1 cut(s) 155
Hin1II CATG 1 cut(s) 528
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HindIII AAGCTT 2 cut(s) 45, 200
HinfI GANTC 1 cut(s) 463
Hpy188I TCNGA 3 cut(s) 106, 216, 370
Hpy188III TCNNGA 5 cut(s) 110, 184, 302, 391, 525
HpyAV CCTTC 2 cut(s) 26, 245
HpyCH4V TGCA 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpyF3I CTNAG 1 cut(s) 389
Hsp92II CATG 1 cut(s) 528
HspAI GCGC 1 cut(s) 153
KpnI GGTACC 1 cut(s) 279
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 1 cut(s) 51
MaeI CTAG 3 cut(s) 15, 110, 296
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 3 cut(s) 154, 253, 509
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 5 cut(s) 36, 138, 383, 394, 500
MlyI GAGTC 1 cut(s) 457
MnlI CCTC 6 cut(s) 69, 237, 367, 372, 396, 409
MseI TTAA 2 cut(s) 228, 291
MslI CAYNNNNRTG 1 cut(s) 230
MspR9I CCNGG 3 cut(s) 321, 330, 345
MvaI CCWGG 3 cut(s) 321, 330, 345
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 325
NdeII GATC 1 cut(s) 106
NlaIII CATG 1 cut(s) 528
NlaIV GGNNCC 3 cut(s) 56, 90, 277
NmeAIII GCCGAG 1 cut(s) 170
PagI TCATGA 1 cut(s) 524
PceI AGGCCT 1 cut(s) 348
PkrI GCNGC 1 cut(s) 66
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 89
PshBI ATTAAT 2 cut(s) 228, 291
PsiI TTATAA 1 cut(s) 450
Psp124BI GAGCTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 89
Psp6I CCWGG 3 cut(s) 319, 328, 343
PspGI CCWGG 3 cut(s) 319, 328, 343
PspN4I GGNNCC 3 cut(s) 56, 90, 277
PspPI GGNCC 1 cut(s) 89
PspPPI RGGWCCY 1 cut(s) 89
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 1 cut(s) 230
SacI GAGCTC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 228, 291
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 3 cut(s) 321, 330, 345
SduI GDGCHC 1 cut(s) 191
SetI ASST 7 cut(s) 49, 191, 204, 256, 277, 301, 334
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 5 cut(s) 36, 138, 383, 394, 500
SseBI AGGCCT 1 cut(s) 348
SspMI CTAG 3 cut(s) 15, 110, 296
SstI GAGCTC 1 cut(s) 191
StuI AGGCCT 1 cut(s) 348
StyD4I CCNGG 3 cut(s) 319, 328, 343
TaqI TCGA 2 cut(s) 303, 455
TasI AATT 5 cut(s) 36, 138, 383, 394, 500
Tru1I TTAA 2 cut(s) 228, 291
Tru9I TTAA 2 cut(s) 228, 291
TseI GCWGC 1 cut(s) 64
TspDTI ATGAA 2 cut(s) 147, 521
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 2 cut(s) 228, 291
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 1 cut(s) 500
XbaI TCTAGA 1 cut(s) 109
XspI CTAG 3 cut(s) 15, 110, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.