Rh5DG520800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008578
Physical Location & Seq
Reverse (-)
80886928 .. 80889603
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG520800.1

Sequence Viewer

Length: 1506 bp
ATGTCGTATCAAGTCCTTGCTTCCATTGGAGAGGCTTGCCCCAATCTCAGGGTGCTTGTGCTAGAATTGGTAGACCAGGAATCACCTGAAGCTTTTAAGATGAACCTGGACAGAATGCTTAATGGTTGTTTATGCTTGGAGTCTTTATCTATAAAGATTCGATGGAGAGGTGTAGGTGCAAATCCTTTACAGCCATTTCGATTCTTCCTGCCTAAAACTCTCAAAAGCATCAAGTTGCAGCCTGTGGTTGAGGAAGACGTAATCTATTTCACTAGTTTGAATGATGCATTTAAGAACTATTTGCCAACAGCTAATGTCAGCACCCCTGTTTCTAAATCCTCATTTGGATTTGCATTGCAGCGCTTGTCGCTTGTCTTAGATGTTATATCAGATCAGCTAATTGTTGTCATTGGCATTTGTGACAATTTGGTAGAGCTGGATCTTGAAGATAGACTAATTCAGGAACCATTGCCAAACCATGACTTAACCAGCGTGGGGCTTATGAGTCTGGCCTCTAATTTTCGTTTGAAGGGATTGTCTCTTGTAAGAAGTAGACAGAACTGCCAAGTCTCGTTTAAGGGAGTGGATGATATTGGCATGTTTGTTTTTTCTAAGTACTGCACGACTCTAGAGTCGGTTAGACTTTATGGGTTCTCCAAAGTTAGTGACGCAGGCTATGCATCAGTCCTACAATCGTATCAAAATTTGAAGAAATTCGAAGTGCGTAATGCCTTCCTTATGTCAGACTTGGCCTTTGTTGACCTTACTCGGTTCTCATGTTCTCTTGTTGAGGTGAAATTGTTGTCATGCTGTCTTATAACTTGTGAAACGGTAAATCAGCTCTCCAGTTCTGGGGTCCTGGAGGTGCTTGATCTGAATGGCTGTAGGAGCATAGCAGACTCCTGCCTCATCTCCATTGCATCGCTTCATAGCTTAACCATGCTGAATCTTTCAGGAGCTGATATTTCTGATTACGGCTTATCTGTTCTTGGGACGGGGAATCCACCCATCACACATTTGTGTCTTAGAAACTGTAAGGATGTGACTGATGAAGGAGTTTTACTTTTGTTTCTTGGAGGAGGCACAATTGGCAAAACACTGTCATCCCTCGATTTAGGACACATACCAGGAATATCTGATAAAGCAGTTCTCACAATTGCTGTGGCCGGTAGACAGATTACTGAGTTGTGTCTCAGGTATTGCTCCTCTATGACTAATTCGTCCCTAGAAGCTTTGGCTACAAGGAAACAGGATCGAGATGATTGTAAACTACTACGGAGGCTTGATCTTTTCAATTGCAAGGGTTTGTCTGCTGACTCATTTAGGTTGCTGAAGAGGCCTTTCTTCCCCGGACTGCACTGGGTTGGTATGGGGAATACTCGTTTGAATAGTAAAGGATGTCCAGCTCTCAGTGAAATTGGTTACGAAAGACCATGGCTGACGATATGTTTGGATGGCTGTGAAATGGGTTGTCATGATGGATGGCAATTCCATAGAGTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

501

Amino Acids

55.21

Weight (kDa)

6.21

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 4 - 493 5.1e-160 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 818
AasI GACNNNNNNGTC 2 cut(s) 631, 1219
AccI GTMKAC 3 cut(s) 72, 553, 1171
AclWI GGATC 2 cut(s) 447, 1260
AcoI YGGCCR 1 cut(s) 1164
AcsI RAATTY 2 cut(s) 703, 713
AcuI CTGAAG 2 cut(s) 108, 1352
AfaI GTAC 1 cut(s) 617
AfeI AGCGCT 1 cut(s) 362
AfiI CCNNNNNNNGG 3 cut(s) 48, 495, 852
AgsI TTSAA 6 cut(s) 280, 446, 529, 709, 1294, 1387
AhlI ACTAGT 1 cut(s) 272
AjnI CCWGG 4 cut(s) 75, 105, 858, 1126
AleI CACNNNNGTG 1 cut(s) 1018
AluBI AGCT 9 cut(s) 92, 311, 397, 436, 841, 933, 959, 1232, 1406
AluI AGCT 9 cut(s) 92, 311, 397, 436, 841, 933, 959, 1232, 1406
Alw26I GTCTC 3 cut(s) 543, 574, 1196
AlwI GGATC 2 cut(s) 447, 1260
AlwNI CAGNNNCTG 1 cut(s) 959
Aor51HI AGCGCT 1 cut(s) 362
AoxI GGCC 4 cut(s) 510, 750, 1164, 1337
ApeKI GCWGC 2 cut(s) 238, 358
ApoI RAATTY 2 cut(s) 703, 713
ArsI GACNNNNNNTTYG 4 cut(s) 737, 769, 1432, 1464
Asp700I GAANNNNTTC 1 cut(s) 713
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 856
AsuC2I CCSGG 1 cut(s) 1350
AsuHPI GGTGA 2 cut(s) 75, 805
AsuII TTCGAA 1 cut(s) 717
AvaII GGWCC 1 cut(s) 856
BaeI ACNNNNGTAYC 2 cut(s) 680, 713
BarI GAAGNNNNNNTAC 2 cut(s) 1044, 1076
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 250, 370
BccI CCATC 5 cut(s) 156, 1016, 1448, 1472, 1476
BceAI ACGGC 1 cut(s) 991
BciT130I CCWGG 4 cut(s) 77, 107, 860, 1128
BcnI CCSGG 1 cut(s) 1350
BcoDI GTCTC 3 cut(s) 543, 574, 1196
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 4 cut(s) 62, 273, 629, 1226
BfmI CTRYAG 1 cut(s) 883
BfoI RGCGCY 1 cut(s) 364
BisI GCNGC 2 cut(s) 239, 359
BlsI GCNGC 2 cut(s) 240, 360
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 5 cut(s) 77, 107, 860, 1128, 1350
Bme18I GGWCC 1 cut(s) 856
BmgT120I GGNCC 1 cut(s) 856
BmiI GGNNCC 2 cut(s) 465, 857
BmrFI CCNGG 5 cut(s) 77, 107, 860, 1128, 1350
BmrI ACTGGG 1 cut(s) 1369
BmsI GCATC 4 cut(s) 237, 274, 689, 929
BmuI ACTGGG 1 cut(s) 1369
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 2 cut(s) 829, 881
Bpu14I TTCGAA 1 cut(s) 717
BpuMI CCSGG 1 cut(s) 1350
BsaJI CCNNGG 2 cut(s) 1348, 1433
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 495, 852
Bse118I RCCGGY 1 cut(s) 1166
Bse1I ACTGG 2 cut(s) 846, 1364
Bse3DI GCAATG 3 cut(s) 353, 467, 915
BseBI CCWGG 4 cut(s) 77, 107, 860, 1128
BseDI CCNNGG 2 cut(s) 1348, 1433
BseGI GGATG 6 cut(s) 592, 1045, 1103, 1403, 1459, 1487
BseLI CCNNNNNNNGG 3 cut(s) 48, 495, 852
BseMI GCAATG 3 cut(s) 353, 467, 915
BseMII CTCAG 4 cut(s) 61, 1173, 1207, 1423
BseNI ACTGG 2 cut(s) 846, 1364
BseRI GAGGAG 2 cut(s) 1092, 1195
BseXI GCAGC 2 cut(s) 250, 370
BsgI GTGCAG 2 cut(s) 604, 1340
BshFI GGCC 4 cut(s) 512, 752, 1166, 1339
BsiSI CCGG 2 cut(s) 1167, 1350
BslFI GGGAC 2 cut(s) 1006, 1207
BslI CCNNNNNNNGG 3 cut(s) 48, 495, 852
BsmAI GTCTC 3 cut(s) 543, 574, 1196
BsmFI GGGAC 2 cut(s) 1006, 1207
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 4 cut(s) 512, 752, 1166, 1339
Bsp119I TTCGAA 1 cut(s) 717
Bsp143I GATC 5 cut(s) 391, 439, 871, 1252, 1285
Bsp19I CCATGG 1 cut(s) 1433
BspANI GGCC 4 cut(s) 512, 752, 1166, 1339
BspCNI CTCAG 4 cut(s) 60, 1174, 1206, 1422
BspHI TCATGA 1 cut(s) 1474
BspLI GGNNCC 2 cut(s) 465, 857
BspPI GGATC 2 cut(s) 447, 1260
BspT104I TTCGAA 1 cut(s) 717
BsrDI GCAATG 3 cut(s) 353, 467, 915
BsrFI RCCGGY 1 cut(s) 1166
BsrI ACTGG 2 cut(s) 846, 1364
BssAI RCCGGY 1 cut(s) 1166
BssECI CCNNGG 2 cut(s) 1348, 1433
BssMI GATC 5 cut(s) 391, 439, 871, 1252, 1285
BssT1I CCWWGG 1 cut(s) 1433
Bst2UI CCWGG 4 cut(s) 77, 107, 860, 1128
Bst4CI ACNGT 3 cut(s) 832, 1034, 1101
Bst6I CTCTTC 1 cut(s) 1328
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 2 cut(s) 37, 673
BstDEI CTNAG 7 cut(s) 47, 376, 612, 1025, 1182, 1193, 1409
BstDSI CCRYGG 1 cut(s) 1433
BstF5I GGATG 6 cut(s) 592, 1045, 1103, 1403, 1459, 1487
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 5 cut(s) 394, 442, 874, 1255, 1288
BstMAI GTCTC 3 cut(s) 543, 574, 1196
BstMBI GATC 5 cut(s) 391, 439, 871, 1252, 1285
BstMWI GCNNNNNNNGC 5 cut(s) 367, 677, 888, 1089, 1336
BstNI CCWGG 4 cut(s) 77, 107, 860, 1128
BstNSI RCATGY 1 cut(s) 601
BstSCI CCNGG 5 cut(s) 75, 105, 858, 1126, 1348
BstSFI CTRYAG 1 cut(s) 883
BstV1I GCAGC 2 cut(s) 250, 370
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 4 cut(s) 512, 752, 1166, 1339
BtgI CCRYGG 1 cut(s) 1433
BtgZI GCGATG 1 cut(s) 906
BtsCI GGATG 6 cut(s) 592, 1045, 1103, 1403, 1459, 1487
BtsIMutI CAGTG 3 cut(s) 1097, 1357, 1417
Cac8I GCNNGC 2 cut(s) 37, 673
CaiI CAGNNNCTG 1 cut(s) 959
CciI TCATGA 1 cut(s) 1474
CfoI GCGC 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 1166
Cfr13I GGNCC 1 cut(s) 856
CseI GACGC 1 cut(s) 677
Csp6I GTAC 1 cut(s) 616
CspCI CAANNNNNGTGG 2 cut(s) 1143, 1178
CviAII CATG 7 cut(s) 479, 598, 777, 807, 940, 1434, 1475
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 7 cut(s) 47, 376, 612, 1025, 1182, 1193, 1409
DpnI GATC 5 cut(s) 393, 441, 873, 1254, 1287
DpnII GATC 5 cut(s) 391, 439, 871, 1252, 1285
DrdI GACNNNNNNGTC 2 cut(s) 631, 1219
DseDI GACNNNNNNGTC 2 cut(s) 631, 1219
EaeI YGGCCR 1 cut(s) 1164
Eam1104I CTCTTC 1 cut(s) 1328
EarI CTCTTC 1 cut(s) 1328
Eco130I CCWWGG 1 cut(s) 1433
Eco147I AGGCCT 1 cut(s) 1339
Eco47I GGWCC 1 cut(s) 856
Eco47III AGCGCT 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 108, 1352
EcoO109I RGGNCCY 1 cut(s) 856
EcoRII CCWGG 4 cut(s) 75, 105, 858, 1126
EcoT14I CCWWGG 1 cut(s) 1433
EcoT22I ATGCAT 2 cut(s) 289, 682
ErhI CCWWGG 1 cut(s) 1433
FaeI CATG 7 cut(s) 482, 601, 780, 810, 943, 1437, 1478
FaqI GGGAC 2 cut(s) 1006, 1207
FatI CATG 7 cut(s) 478, 597, 776, 806, 939, 1433, 1474
FblI GTMKAC 3 cut(s) 72, 553, 1171
Fnu4HI GCNGC 2 cut(s) 239, 359
FokI GGATG 6 cut(s) 599, 1052, 1090, 1410, 1466, 1494
Fsp4HI GCNGC 2 cut(s) 239, 359
FspBI CTAG 4 cut(s) 62, 273, 629, 1226
GlaI GCGC 1 cut(s) 362
GluI GCNGC 2 cut(s) 239, 359
GsuI CTGGAG 2 cut(s) 829, 881
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 4 cut(s) 512, 752, 1166, 1339
HapII CCGG 2 cut(s) 1167, 1350
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 363
Hin1II CATG 7 cut(s) 482, 601, 780, 810, 943, 1437, 1478
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 2 cut(s) 90, 1230
HpaII CCGG 2 cut(s) 1167, 1350
HphI GGTGA 2 cut(s) 75, 805
Hpy166II GTNNAC 5 cut(s) 73, 554, 760, 1172, 1268
Hpy188I TCNGA 5 cut(s) 391, 745, 876, 970, 1138
Hpy188III TCNNGA 6 cut(s) 443, 461, 629, 954, 1256, 1475
Hpy8I GTNNAC 5 cut(s) 73, 554, 760, 1172, 1268
HpyAV CCTTC 3 cut(s) 523, 742, 1046
HpyCH4III ACNGT 3 cut(s) 832, 1034, 1101
HpyCH4IV ACGT 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 5 cut(s) 367, 677, 888, 1089, 1336
HpyF3I CTNAG 7 cut(s) 47, 376, 612, 1025, 1182, 1193, 1409
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 7 cut(s) 482, 601, 780, 810, 943, 1437, 1478
HspAI GCGC 1 cut(s) 361
Kzo9I GATC 5 cut(s) 391, 439, 871, 1252, 1285
LmnI GCTCC 3 cut(s) 888, 956, 1208
Lsp1109I GCAGC 2 cut(s) 250, 370
LweI GCATC 4 cut(s) 237, 274, 689, 929
MaeI CTAG 4 cut(s) 62, 273, 629, 1226
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 4 cut(s) 419, 665, 1042, 1421
MalI GATC 5 cut(s) 393, 441, 873, 1254, 1287
MboI GATC 5 cut(s) 391, 439, 871, 1252, 1285
MboII GAAGA 6 cut(s) 196, 266, 458, 721, 1336, 1345
MfeI CAATTG 3 cut(s) 1086, 1155, 1294
MflI RGATCY 1 cut(s) 439
MlyI GAGTC 6 cut(s) 149, 514, 619, 641, 893, 1310
Mph1103I ATGCAT 2 cut(s) 289, 682
MroXI GAANNNNTTC 1 cut(s) 713
MseI TTAA 6 cut(s) 96, 120, 291, 485, 576, 935
MslI CAYNNNNRTG 1 cut(s) 1018
MspI CCGG 2 cut(s) 1167, 1350
MspR9I CCNGG 5 cut(s) 77, 107, 860, 1128, 1350
MunI CAATTG 3 cut(s) 1086, 1155, 1294
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 4 cut(s) 77, 107, 860, 1128
MwoI GCNNNNNNNGC 5 cut(s) 367, 677, 888, 1089, 1336
NciI CCSGG 1 cut(s) 1350
NcoI CCATGG 1 cut(s) 1433
NdeII GATC 5 cut(s) 391, 439, 871, 1252, 1285
NlaIII CATG 7 cut(s) 482, 601, 780, 810, 943, 1437, 1478
NlaIV GGNNCC 2 cut(s) 465, 857
NmuCI GTSAC 3 cut(s) 419, 665, 1042
NsiI ATGCAT 2 cut(s) 289, 682
NspI RCATGY 1 cut(s) 601
NspV TTCGAA 1 cut(s) 717
OliI CACNNNNGTG 1 cut(s) 1018
PagI TCATGA 1 cut(s) 1474
PceI AGGCCT 1 cut(s) 1339
PctI GAATGC 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 713
PfeI GAWTC 5 cut(s) 80, 157, 201, 946, 1000
PfoI TCCNGGA 1 cut(s) 858
PkrI GCNGC 2 cut(s) 240, 360
PleI GAGTC 6 cut(s) 148, 513, 619, 640, 893, 1310
PpsI GAGTC 6 cut(s) 148, 513, 619, 640, 893, 1310
PpuMI RGGWCCY 1 cut(s) 856
PsiI TTATAA 1 cut(s) 818
Psp5II RGGWCCY 1 cut(s) 856
Psp6I CCWGG 4 cut(s) 75, 105, 858, 1126
PspGI CCWGG 4 cut(s) 75, 105, 858, 1126
PspN4I GGNNCC 2 cut(s) 465, 857
PspPI GGNCC 1 cut(s) 856
PspPPI RGGWCCY 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 959
PsuI RGATCY 1 cut(s) 439
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 1 cut(s) 1018
SaqAI TTAA 6 cut(s) 96, 120, 291, 485, 576, 935
SatI GCNGC 2 cut(s) 239, 359
Sau3AI GATC 5 cut(s) 391, 439, 871, 1252, 1285
Sau96I GGNCC 1 cut(s) 856
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 6 cut(s) 149, 514, 619, 641, 893, 1310
ScrFI CCNGG 5 cut(s) 77, 107, 860, 1128, 1350
SfaNI GCATC 4 cut(s) 237, 274, 689, 929
SfcI CTRYAG 1 cut(s) 883
SfuI TTCGAA 1 cut(s) 717
SinI GGWCC 1 cut(s) 856
SmiMI CAYNNNNRTG 1 cut(s) 1018
SpeI ACTAGT 1 cut(s) 272
SseBI AGGCCT 1 cut(s) 1339
SspMI CTAG 4 cut(s) 62, 273, 629, 1226
StuI AGGCCT 1 cut(s) 1339
StyD4I CCNGG 5 cut(s) 75, 105, 858, 1126, 1348
StyI CCWWGG 1 cut(s) 1433
TaaI ACNGT 3 cut(s) 832, 1034, 1101
TaiI ACGT 1 cut(s) 261
TaqI TCGA 5 cut(s) 160, 199, 717, 1110, 1255
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 5 cut(s) 80, 157, 201, 946, 1000
Tru1I TTAA 6 cut(s) 96, 120, 291, 485, 576, 935
Tru9I TTAA 6 cut(s) 96, 120, 291, 485, 576, 935
TscAI CASTG 3 cut(s) 1104, 1364, 1417
TseFI GTSAC 3 cut(s) 419, 665, 1042
TseI GCWGC 2 cut(s) 238, 358
Tsp45I GTSAC 3 cut(s) 419, 665, 1042
TspDTI ATGAA 3 cut(s) 116, 917, 1065
TspGWI ACGGA 1 cut(s) 1291
TspRI CASTG 3 cut(s) 1104, 1364, 1417
VpaK11BI GGWCC 1 cut(s) 856
XapI RAATTY 2 cut(s) 703, 713
XbaI TCTAGA 1 cut(s) 628
XceI RCATGY 1 cut(s) 601
XmiI GTMKAC 3 cut(s) 72, 553, 1171
XmnI GAANNNNTTC 1 cut(s) 713
XspI CTAG 4 cut(s) 62, 273, 629, 1226
ZrmI AGTACT 1 cut(s) 617
Zsp2I ATGCAT 2 cut(s) 289, 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.