MD11G1063100.v1.1

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013042
Physical Location & Seq
Reverse (-)
5471408 .. 5473179
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1063100.v1.1.491

Sequence Viewer

Length: 705 bp
ATGAGGTACCATTCTTTCTTAAGAAGCCCAAAGAAGGAAGAGCAACAAGTGCCGGACTGTACCCATAATTCTGGTGAAGAATTCTCTGACACTACTAATACAAAATTAGATTGTGTTTACACCAATGGAGACAATGATCATGAGGTTGATATCATGGATGATTGGGAGCGAATTTTGGGAATCGAAGAGGAAGAAGATGGAAAATTGCCGAGCGATAACATGTACAGCGGGCACAAGCTCAATTGGGATTTTATGGATTGGGATGAGTTTCCCAATGGAGAAGAAGAAGGGGAGGAGATAGAGCAAAAGGTTTATCAAGGCACTAATAAGTGCTTTTTTGAAGAGGAGAGCTACTACAAAAAAAAAGTAGTAAAGAGTGAGAGTGTAGGGTTTTGGGATGATGATGATGAGAAGAAGGTGCAACTGAATTTGAATTTGAATTATCAAGAGGTTTTGGATGCTTGGTCGGATCGTGGTTCTCTTTGGGCAGATGATTGTTCACTTTCTTCCATGGCTAGCAATGGCAATTATATGGGCGAGGTGCCGGTAATGGAAGAAGACAAGACAAGAAGAGAGGCAAGTGTTCTAAGGTACAAGGAGAAACGCCAGTCTAGGCTCTTCTCCAAAAAGATAAGGTACCAAGTCCGCAAGCTCAATGCAGATAAAAGACCTAGACTCAAGGGTCGATTTGTGAAGAGGGTTTGA

Protein Analysis

235

Amino Acids

27.72

Weight (kDa)

4.9

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCT PF06203 191 - 233 2.8e-18 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013042)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07050 AT1G07050
fragaria_vesca FvH4_3g39550
malus_domestica MD03G1059900.v1.1 MD11G1063100.v1.1
prunus_persica Prupe.6G050300_v2.0.a1 Prupe.6G050300_v2.0.a1
pyrus_communis pycom11g05280
rosa_chinensis RchiOBHm_Chr5g0071321
rosa_laevigata RLG00000036202
rosa_multiflora Rmu_sc0006990.1_g000005
rosa_roxburghii Rroxscaffold_1G00009810
rosa_rugosa Rorug05G0411300
rosa_samantha Rh5AG467700 Rh5BG486200 Rh5CG510600 Rh5DG497400
rosa_wichuraiana Rw5G043450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 681
Acc65I GGTACC 2 cut(s) 6, 636
AccB1I GGYRCC 3 cut(s) 6, 541, 636
AciI CCGC 2 cut(s) 228, 646
AclWI GGATC 1 cut(s) 477
AcsI RAATTY 4 cut(s) 80, 171, 427, 433
AfaI GTAC 5 cut(s) 8, 61, 224, 593, 638
AfiI CCNNNNNNNGG 1 cut(s) 34
AflII CTTAAG 1 cut(s) 19
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 3 cut(s) 341, 433, 439
AluBI AGCT 3 cut(s) 238, 351, 652
AluI AGCT 3 cut(s) 238, 351, 652
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 1 cut(s) 477
ApoI RAATTY 4 cut(s) 80, 171, 427, 433
Asp718I GGTACC 2 cut(s) 6, 636
AsuHPI GGTGA 1 cut(s) 86
AsuNHI GCTAGC 1 cut(s) 515
BaeGI GKGCMC 1 cut(s) 234
BanI GGYRCC 3 cut(s) 6, 541, 636
BbsI GAAGAC 1 cut(s) 564
BccI CCATC 1 cut(s) 191
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 3 cut(s) 516, 612, 672
BfrI CTTAAG 1 cut(s) 19
BmiI GGNNCC 3 cut(s) 8, 543, 638
BmsI GCATC 1 cut(s) 448
BmtI GCTAGC 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 510
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 1 cut(s) 607
Bse3DI GCAATG 1 cut(s) 526
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 4 cut(s) 163, 268, 403, 463
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 526
BseNI ACTGG 1 cut(s) 607
BseRI GAGGAG 2 cut(s) 308, 359
BseSI GKGCMC 1 cut(s) 234
BshNI GGYRCC 3 cut(s) 6, 541, 636
BsiSI CCGG 2 cut(s) 53, 545
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 234
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 2 cut(s) 136, 469
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 2 cut(s) 228, 646
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 3 cut(s) 8, 543, 638
BspOI GCTAGC 1 cut(s) 519
BspPI GGATC 1 cut(s) 477
BspQI GCTCTTC 2 cut(s) 33, 623
BspT107I GGYRCC 3 cut(s) 6, 541, 636
BspTI CTTAAG 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 526
BsrFI RCCGGY 1 cut(s) 544
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 1 cut(s) 607
BssAI RCCGGY 1 cut(s) 544
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 2 cut(s) 136, 469
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 6 cut(s) 33, 180, 336, 565, 623, 689
BstAFI CTTAAG 1 cut(s) 19
BstAPI GCANNNNNTGC 1 cut(s) 49
BstAUI TGTACA 1 cut(s) 222
BstC8I GCNNGC 3 cut(s) 230, 517, 650
BstDEI CTNAG 1 cut(s) 587
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 4 cut(s) 163, 268, 403, 463
BstKTI GATC 2 cut(s) 139, 472
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 2 cut(s) 136, 469
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNSI RCATGY 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 234
BstV2I GAAGAC 1 cut(s) 564
BstXI CCANNNNNNTGG 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 4 cut(s) 163, 268, 403, 463
Cac8I GCNNGC 3 cut(s) 230, 517, 650
CciI TCATGA 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 544
Csp6I GTAC 5 cut(s) 7, 60, 223, 592, 637
CviAII CATG 4 cut(s) 140, 154, 220, 511
CviJI RGCY 6 cut(s) 27, 238, 351, 515, 616, 652
CviKI_1 RGCY 6 cut(s) 27, 238, 351, 515, 616, 652
CviQI GTAC 5 cut(s) 7, 60, 223, 592, 637
DdeI CTNAG 1 cut(s) 587
DpnI GATC 2 cut(s) 138, 471
DpnII GATC 2 cut(s) 136, 469
DrdI GACNNNNNNGTC 1 cut(s) 681
DseDI GACNNNNNNGTC 1 cut(s) 681
Eam1104I CTCTTC 6 cut(s) 33, 180, 336, 565, 623, 689
EarI CTCTTC 6 cut(s) 33, 180, 336, 565, 623, 689
Eco130I CCWWGG 1 cut(s) 510
Eco32I GATATC 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 80
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 4 cut(s) 143, 157, 223, 514
FaiI YATR 8 cut(s) 66, 141, 155, 221, 254, 512, 531, 533
FatI CATG 4 cut(s) 139, 153, 219, 510
FauI CCCGC 1 cut(s) 221
FbaI TGATCA 1 cut(s) 136
FokI GGATG 4 cut(s) 170, 275, 410, 470
FspBI CTAG 3 cut(s) 516, 612, 672
HapII CCGG 2 cut(s) 53, 545
Hin1II CATG 4 cut(s) 143, 157, 223, 514
HinfI GANTC 2 cut(s) 180, 675
HpaII CCGG 2 cut(s) 53, 545
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 2 cut(s) 118, 500
Hpy188I TCNGA 2 cut(s) 88, 469
Hpy188III TCNNGA 2 cut(s) 140, 446
Hpy8I GTNNAC 2 cut(s) 118, 500
HpyAV CCTTC 3 cut(s) 28, 281, 409
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 421, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 1 cut(s) 587
Hsp92II CATG 4 cut(s) 143, 157, 223, 514
KpnI GGTACC 2 cut(s) 10, 640
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 136, 469
LguI GCTCTTC 2 cut(s) 33, 623
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 4 cut(s) 57, 66, 558, 620
LweI GCATC 1 cut(s) 448
MaeI CTAG 3 cut(s) 516, 612, 672
MalI GATC 2 cut(s) 138, 471
MboI GATC 2 cut(s) 136, 469
MfeI CAATTG 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 234
MlyI GAGTC 1 cut(s) 669
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 8 cut(s) 136, 181, 286, 337, 442, 532, 568, 690
MseI TTAA 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 228
MspCI CTTAAG 1 cut(s) 19
MspI CCGG 2 cut(s) 53, 545
MunI CAATTG 1 cut(s) 241
MwoI GCNNNNNNNGC 1 cut(s) 49
NcoI CCATGG 1 cut(s) 510
NdeII GATC 2 cut(s) 136, 469
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 4 cut(s) 143, 157, 223, 514
NlaIV GGNNCC 3 cut(s) 8, 543, 638
NmeAIII GCCGAG 1 cut(s) 234
NspI RCATGY 1 cut(s) 223
PagI TCATGA 1 cut(s) 139
PciI ACATGT 1 cut(s) 219
PciSI GCTCTTC 2 cut(s) 33, 623
PfeI GAWTC 1 cut(s) 180
PleI GAGTC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 669
PscI ACATGT 1 cut(s) 219
PspN4I GGNNCC 3 cut(s) 8, 543, 638
RsaI GTAC 5 cut(s) 8, 61, 224, 593, 638
RsaNI GTAC 5 cut(s) 7, 60, 223, 592, 637
SapI GCTCTTC 2 cut(s) 33, 623
SaqAI TTAA 1 cut(s) 20
Sau3AI GATC 2 cut(s) 136, 469
SchI GAGTC 1 cut(s) 669
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 1 cut(s) 448
SmlI CTYRAG 2 cut(s) 19, 677
SmoI CTYRAG 2 cut(s) 19, 677
SsiI CCGC 2 cut(s) 228, 646
SspMI CTAG 3 cut(s) 516, 612, 672
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 1 cut(s) 59
TaqI TCGA 2 cut(s) 183, 685
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
Vha464I CTTAAG 1 cut(s) 19
XapI RAATTY 4 cut(s) 80, 171, 427, 433
XceI RCATGY 1 cut(s) 223
XspI CTAG 3 cut(s) 516, 612, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.