MD11G1068500.v1.1

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008317
Physical Location & Seq
Reverse (-)
5895570 .. 5896543
974 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1068500.v1.1.491

Sequence Viewer

Length: 771 bp
ATGTTAGACCATAAAGAAGTTATCATATCCCATTTAAGTTGGGCCAGTCTCTTTTTAGGGTTCCATACTTTTGGACTTTATGTGTATAATGATGTCATGCTTGCTTTTGCGTTCAATGCGGGTCGAAGCATATGGTTACCTAGTTGGTTAAATGCTATTAATGAGAATAGTAATTCACTATTCTTATCAATAGGACGTGGAGATTTCTTGGTTCATCATGCCATTGCTCTAGGTTTACATATAACTACATTGATATTAGTAAAAGGTGCTTTAGATGCACGTGGTTCCAAGTTAATGCCAAATAAAAAATATTTGGTTACAATTTTCCTTGCGATGGTCCGGGACGAGCATTGGAAGCACATCACATTATGGCAGGGTAACGTTTCACAGTTTAATGAGTCTTCCACTTATTTGATGGGATGGTTAAGAGATTATCTATGGTTAAACTCTTCACAACTTATCAATGGATATAACCCTTTTGGTATGAATAGTTTATCAATCTGGGTGTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACGGGATTTATGTTCTTAATTTCCTGGCGTGGATATTGGCAAGAATTGATTGAAACTTTAGCATGGGCTCATGAACACACACCTTTGGCTAATTTGATTCGATGGAGAGATAAACCGGTGGCTCTTTCCATTGTGCAAGCAAGATTGGTTGGATTAGCCCACTTTTCTGTTGGATATATATTTACTTATGCGGCTTTCTTGATTGCCTCTACATCGGGCAAATTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaA_PsaB

Protein Analysis

257

Amino Acids

29.36

Weight (kDa)

9.13

Isoelectric Point (pI)

25.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaA_PsaB PF00223 1 - 37 1.1e-13 Photosystem I psaA/psaB protein
PsaA_PsaB PF00223 42 - 105 1.2e-22 Photosystem I psaA/psaB protein
PsaA_PsaB PF00223 117 - 249 1.1e-70 Photosystem I psaA/psaB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 119, 731
AclI AACGTT 1 cut(s) 381
AcsI RAATTY 1 cut(s) 761
AcvI CACGTG 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 334
AgeI ACCGGT 1 cut(s) 655
AgsI TTSAA 2 cut(s) 115, 593
AjiI CACGTC 1 cut(s) 197
AjnI CCWGG 1 cut(s) 563
Alw26I GTCTC 1 cut(s) 53
AoxI GGCC 1 cut(s) 42
ApoI RAATTY 1 cut(s) 761
ArsI GACNNNNNNTTYG 2 cut(s) 516, 548
AseI ATTAAT 1 cut(s) 159
AsiGI ACCGGT 1 cut(s) 655
AspS9I GGNCC 2 cut(s) 42, 337
AsuC2I CCSGG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 610
BbrPI CACGTG 1 cut(s) 281
BbsI GAAGAC 1 cut(s) 393
BccI CCATC 4 cut(s) 328, 409, 414, 636
BciT130I CCWGG 1 cut(s) 565
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 141, 230
BisI GCNGC 1 cut(s) 732
BlsI GCNGC 1 cut(s) 733
Bme1390I CCNGG 2 cut(s) 341, 565
Bme18I GGWCC 1 cut(s) 337
BmgBI CACGTC 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 42, 337
BmiI GGNNCC 2 cut(s) 62, 286
BmrFI CCNGG 2 cut(s) 341, 565
BmsI GCATC 1 cut(s) 265
BpiI GAAGAC 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 341
BsaAI YACGTR 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 655
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse118I RCCGGY 1 cut(s) 655
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 222
BseBI CCWGG 1 cut(s) 565
BseGI GGATG 2 cut(s) 425, 516
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMI GCAATG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 45
BshFI GGCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 655
BsiSI CCGG 2 cut(s) 340, 656
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 610
BspACI CCGC 2 cut(s) 119, 731
BspANI GGCC 1 cut(s) 44
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 2 cut(s) 62, 286
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 655
BsrI ACTGG 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 655
Bst2UI CCWGG 1 cut(s) 565
Bst4CI ACNGT 1 cut(s) 390
Bst6I CTCTTC 1 cut(s) 454
BstBAI YACGTR 1 cut(s) 281
BstC8I GCNNGC 2 cut(s) 102, 678
BstEII GGTNACC 1 cut(s) 135
BstF5I GGATG 2 cut(s) 425, 516
BstMAI GTCTC 1 cut(s) 53
BstMWI GCNNNNNNNGC 3 cut(s) 116, 275, 355
BstNI CCWGG 1 cut(s) 565
BstPI GGTNACC 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 339, 563
BstV2I GAAGAC 1 cut(s) 393
BstXI CCANNNNNNTGG 1 cut(s) 71
BsuRI GGCC 1 cut(s) 44
BtgZI GCGATG 1 cut(s) 347
BtrI CACGTC 1 cut(s) 197
BtsCI GGATG 2 cut(s) 425, 516
Cac8I GCNNGC 2 cut(s) 102, 678
CciI TCATGA 1 cut(s) 610
Cfr10I RCCGGY 1 cut(s) 655
Cfr13I GGNCC 2 cut(s) 42, 337
CspAI ACCGGT 1 cut(s) 655
CviAII CATG 4 cut(s) 97, 218, 603, 611
CviJI RGCY 7 cut(s) 44, 539, 608, 629, 662, 698, 734
CviKI_1 RGCY 7 cut(s) 44, 539, 608, 629, 662, 698, 734
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 337
Eco72I CACGTG 1 cut(s) 281
Eco91I GGTNACC 1 cut(s) 135
EcoO65I GGTNACC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 610
FaeI CATG 4 cut(s) 100, 221, 606, 614
FaqI GGGAC 1 cut(s) 356
FatI CATG 4 cut(s) 96, 217, 602, 610
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 131
Fnu4HI GCNGC 1 cut(s) 732
FokI GGATG 2 cut(s) 432, 523
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 1 cut(s) 732
FspBI CTAG 2 cut(s) 141, 230
GluI GCNGC 1 cut(s) 732
HaeIII GGCC 1 cut(s) 44
HapII CCGG 2 cut(s) 340, 656
Hin1II CATG 4 cut(s) 100, 221, 606, 614
HinfI GANTC 2 cut(s) 398, 637
HpaII CCGG 2 cut(s) 340, 656
Hpy166II GTNNAC 1 cut(s) 236
Hpy188III TCNNGA 2 cut(s) 611, 739
Hpy8I GTNNAC 1 cut(s) 236
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4IV ACGT 3 cut(s) 196, 280, 381
HpyCH4V TGCA 2 cut(s) 278, 676
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 275, 355
HpySE526I ACGT 3 cut(s) 196, 280, 381
Hsp92II CATG 4 cut(s) 100, 221, 606, 614
LpnPI CCDG 7 cut(s) 58, 353, 359, 487, 550, 577, 669
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 141, 230
MaeII ACGT 3 cut(s) 196, 280, 381
MaeIII GTNAC 3 cut(s) 135, 316, 377
MboII GAAGA 2 cut(s) 393, 441
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 6 cut(s) 172, 321, 558, 584, 631, 761
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 670, 691
MnlI CCTC 1 cut(s) 757
MseI TTAA 9 cut(s) 35, 149, 159, 293, 393, 425, 443, 557, 769
MspI CCGG 2 cut(s) 340, 656
MspR9I CCNGG 2 cut(s) 341, 565
MvaI CCWGG 1 cut(s) 565
MwoI GCNNNNNNNGC 3 cut(s) 116, 275, 355
NciI CCSGG 1 cut(s) 341
NdeI CATATG 1 cut(s) 131
NlaIII CATG 4 cut(s) 100, 221, 606, 614
NlaIV GGNNCC 2 cut(s) 62, 286
PagI TCATGA 1 cut(s) 610
PfeI GAWTC 1 cut(s) 637
PfoI TCCNGGA 1 cut(s) 339
PinAI ACCGGT 1 cut(s) 655
PkrI GCNGC 1 cut(s) 733
PleI GAGTC 1 cut(s) 406
PmaCI CACGTG 1 cut(s) 281
PmlI CACGTG 1 cut(s) 281
PpsI GAGTC 1 cut(s) 406
Ppu21I YACGTR 1 cut(s) 281
PshBI ATTAAT 1 cut(s) 159
Psp1406I AACGTT 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 563
PspCI CACGTG 1 cut(s) 281
PspEI GGTNACC 1 cut(s) 135
PspGI CCWGG 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 62, 286
PspPI GGNCC 2 cut(s) 42, 337
SaqAI TTAA 9 cut(s) 35, 149, 159, 293, 393, 425, 443, 557, 769
SatI GCNGC 1 cut(s) 732
Sau96I GGNCC 2 cut(s) 42, 337
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 341, 565
SduI GDGCHC 1 cut(s) 610
SetI ASST 7 cut(s) 142, 199, 235, 268, 283, 384, 625
SfaNI GCATC 1 cut(s) 265
SinI GGWCC 1 cut(s) 337
Sse9I AATT 6 cut(s) 172, 321, 558, 584, 631, 761
SsiI CCGC 2 cut(s) 119, 731
SspI AATATT 1 cut(s) 311
SspMI CTAG 2 cut(s) 141, 230
StyD4I CCNGG 2 cut(s) 339, 563
TaaI ACNGT 1 cut(s) 390
TaiI ACGT 3 cut(s) 199, 283, 384
TaqI TCGA 2 cut(s) 124, 640
TasI AATT 6 cut(s) 172, 321, 558, 584, 631, 761
TauI GCSGC 1 cut(s) 734
TfiI GAWTC 1 cut(s) 637
Tru1I TTAA 9 cut(s) 35, 149, 159, 293, 393, 425, 443, 557, 769
Tru9I TTAA 9 cut(s) 35, 149, 159, 293, 393, 425, 443, 557, 769
TspDTI ATGAA 3 cut(s) 203, 500, 627
VpaK11BI GGWCC 1 cut(s) 337
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 1 cut(s) 761
XcmI CCANNNNNNNNNTGG 2 cut(s) 412, 707
XspI CTAG 2 cut(s) 141, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.