pycom12401g00190

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012401
OG0008317
Physical Location & Seq
Reverse (-)
86330 .. 88615
2286 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12401g00190.1

Sequence Viewer

Length: 2064 bp
ATGCGCCTTCTGTTGACTTCGACGTTGCGAAAAGGTGCCCCCAAGCCCGGGGATCTCTTAATAAAGGGTAGGCTACGAGGTTATCGTACGGACCTCCTCCCCAATACGAAGATAGTCCCGCCCCAGGTAGCTTGTCTCCTCTATGTCGAGCTGATCCTAGGGCGCCTTTCTGCTGGATCCCGTTTAAAGTCTGGTTTTGTTATTCCCATGGGAGTGGAAAAGCAATTGAGGCCAAAAACACGGGGTTTCGAGCGATGGGCCAGACCCGGTCATTTCTCAAGAACAATAGCTAAAGGGCCTGATACTACCACTTGGATCTGGAACCTACATGCTGATGCTCACGATTTCGATAGCCATACTAATGATTTGGAGGATATCTCTCAAAAGTATTTATGGCATGTATTTCCACGGTGCTCTTTTTCCAATTATGAAGCATGGTTAAGCGATCCTACTCACATTGGACCTAGTGCCCAGGTGGTTTGGCCAATAGTGGGCCAAGAAATATTGAATGGTGATATTTGGCGAGCATCCGGAATAACTAGTGAATTACAACTCTATTGTACCGCAATTGGTGCATTGGTCTTTGCAGCTTTAATGCTTTTTGCTGGTTGGTTTCATTACCACAAAGCTGCTCCAAAATTGGCTTGGTTCCAAGATGTAGAATCTATGTTGAATCACCATTTAGCAGGGCTACTAGGGCTTGGGTCTCTTTCTTGGGCGGGGCATCAAGTACATGTATCTTTACCTATTAACCAATTTCTAAATGCTGGAGTAGATCCTAAAGAGATCCCACTTCCTCATGAATTTATCTTGAATCGGGATCTTTTGGCTCAACTTTATCCCAGTTTTGCTGAGGGAGCAACCCCATTTTTAACCTTGAATTGGTCAAAATATGCGGAATTTCTTACTTTTCGTGGAGGATTAGATCCAGTAACTGGGGGTCTATGGCTAACCGATATTGCACACCATCATTTAGCTATTGCAATTCTTTTCCTGATAGCGGGTCACATGTATAGGACCAACTGGGGCATTGGTCATGGTATAAAAGAGATTTTAGAGGCTCATAAAGGTCCATTTACAGGCCAGGGCCATAAAGGCCTATATGAGATCCTAACAACGTCATGGCATGCTCAATTATCTATTAACCTAGCCATGTTAGGTTCTTTAACCATTGTTGTAGCTCACCATATGTATTCGATGCCCCCTTATCCATATCTAGCGACTGACTATGGTACCCAACTGTCATTGTTCACACATCATATGTGGATTGGTGGATTTCTCATAGTTGGTGCTGCTGCGCATGCAGCCATTTTTATGGTAAGAGACTATAATCCAACTACTGGATACAATAACCTCTTAGATCGTGTACTTAGGCATCGCGATGCAATCATATCACACCTCAACTGGGTATGTATATTTTTAGGCTTTCACAGTTTTGGTTTGTATATTCATAATGATACCATGAGCGCTTTAGGGCGTCCCCAAGATATGTTTTCAGATACCGCTATACAATTACAACCCGTCTTTGCTCAATGGATACAAAACACTCATTCTTTAGCACCCGGTGCAACAGCCCCTGGTGCAACAACAAGCACCAGTTTGACTTGGGGGGGTGCTGATTTAGTGGCAGTGGGCGGAAAAGTTGCTTTGTTACCTATTCCATTAGGAACCGCAGATTTTTTGGTACATCACATTCATGCATTTACGATTCATGTGACGGTATTGATACTCCTGAAAGGTGTTCTATTTGCTCGTAGCTCTCGTTTGATACCGGATAAAGCAAATCTTGGTTTTCGTTTCCCTTGTGATGGACCTGGAAGGGGGGGTACATGCCAAGTATCCGCTTGGGATCATGTCTTTTTAGGGCTATTCTGGATGTACAATGCAATTTCGGTAGTAATCTTCCATTTCAGTTGGAAAATGCAGTCAGACGTTTGGGGTAGTATAAGTGATCAAGGGGTAGTAACTCATATCACGGGAGGAAACTTTGCGCAGAGTTCCATTACTATTAATGGGTGGCCCCTTGCGCGCTTTAGGAGGAGCCCAGTTCCAGAAGGAAAGTAA

Protein Analysis

688

Amino Acids

76.15

Weight (kDa)

8.73

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaA_PsaB PF00223 92 - 673 1.1e-257 Photosystem I psaA/psaB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1299, 1992
Acc65I GGTACC 1 cut(s) 1232
AccB1I GGYRCC 3 cut(s) 35, 162, 1232
AccB7I CCANNNNNTGG 2 cut(s) 935, 1340
AccII CGCG 2 cut(s) 1380, 2029
AccIII TCCGGA 1 cut(s) 530
AciI CCGC 9 cut(s) 119, 564, 719, 896, 1001, 1503, 1635, 1671, 1842
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 2 cut(s) 803, 899
AcyI GRCGYC 2 cut(s) 163, 1477
AdeI CACNNNGTG 1 cut(s) 1565
AfaI GTAC 8 cut(s) 88, 562, 732, 1234, 1368, 1686, 1828, 1880
AfeI AGCGCT 1 cut(s) 1468
AflIII ACRYGT 2 cut(s) 733, 1008
AgsI TTSAA 4 cut(s) 508, 673, 814, 880
AhlI ACTAGT 1 cut(s) 539
AjnI CCWGG 5 cut(s) 123, 471, 1083, 1576, 1813
AluBI AGCT 8 cut(s) 131, 151, 290, 590, 629, 977, 1181, 1758
AluI AGCT 8 cut(s) 131, 151, 290, 590, 629, 977, 1181, 1758
Alw21I GWGCWC 1 cut(s) 416
Alw26I GTCTC 3 cut(s) 140, 711, 1317
AlwNI CAGNNNCTG 2 cut(s) 935, 1577
Ama87I CYCGRG 1 cut(s) 47
Aor13HI TCCGGA 1 cut(s) 530
Aor51HI AGCGCT 1 cut(s) 1468
AoxI GGCC 9 cut(s) 230, 258, 296, 482, 493, 1081, 1087, 1096, 2018
ApeKI GCWGC 5 cut(s) 587, 629, 1292, 1295, 1304
ApoI RAATTY 2 cut(s) 803, 899
AseI ATTAAT 1 cut(s) 2010
Asp718I GGTACC 1 cut(s) 1232
AspA2I CCTAGG 1 cut(s) 157
AspLEI GCGC 7 cut(s) 6, 165, 1300, 1469, 1993, 2029, 2031
AsuC2I CCSGG 4 cut(s) 48, 49, 267, 1563
AsuHPI GGTGA 3 cut(s) 524, 668, 1175
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 5 cut(s) 91, 461, 1017, 1070, 1811
AvrII CCTAGG 1 cut(s) 157
BaeGI GKGCMC 2 cut(s) 40, 472
BaeI ACNNNNGTAYC 2 cut(s) 1216, 1249
BalI TGGCCA 1 cut(s) 484
BamHI GGATCC 1 cut(s) 176
BanI GGYRCC 3 cut(s) 35, 162, 1232
BanII GRGCYC 1 cut(s) 2045
BarI GAAGNNNNNNTAC 2 cut(s) 1810, 1842
Bbv12I GWGCWC 1 cut(s) 416
BbvCI CCTCAGC 1 cut(s) 852
BbvI GCAGC 5 cut(s) 599, 616, 1279, 1282, 1316
BccI CCATC 3 cut(s) 249, 975, 1802
BciT130I CCWGG 5 cut(s) 125, 473, 1085, 1578, 1815
BciVI GTATCC 3 cut(s) 1337, 1530, 1849
BclI TGATCA 1 cut(s) 1951
BcnI CCSGG 4 cut(s) 48, 49, 267, 1563
BcoDI GTCTC 3 cut(s) 140, 711, 1317
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 6 cut(s) 158, 465, 540, 695, 1148, 1217
BfoI RGCGCY 2 cut(s) 166, 1470
BfuI GTATCC 3 cut(s) 1337, 1530, 1849
BglI GCCNNNNNGGC 1 cut(s) 1095
BisI GCNGC 5 cut(s) 588, 630, 1293, 1296, 1305
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 5 cut(s) 589, 631, 1294, 1297, 1306
Bme1390I CCNGG 9 cut(s) 48, 49, 125, 267, 473, 1085, 1563, 1578, 1815
Bme18I GGWCC 5 cut(s) 91, 461, 1017, 1070, 1811
BmeT110I CYCGRG 1 cut(s) 47
BmiI GGNNCC 9 cut(s) 37, 164, 178, 323, 650, 1234, 1669, 2021, 2042
BmrFI CCNGG 9 cut(s) 48, 49, 125, 267, 473, 1085, 1563, 1578, 1815
BmrI ACTGGG 5 cut(s) 837, 945, 1033, 1414, 2039
BmsI GCATC 6 cut(s) 325, 536, 733, 1188, 1372, 1384
BmuI ACTGGG 5 cut(s) 837, 945, 1033, 1414, 2039
BplI GAGNNNNNCTC 2 cut(s) 362, 394
BpmI CTGGAG 1 cut(s) 789
Bpu10I CCTNAGC 1 cut(s) 852
BpuEI CTTGAG 1 cut(s) 262
BpuMI CCSGG 4 cut(s) 48, 49, 267, 1563
BsaHI GRCGYC 2 cut(s) 163, 1477
BsaI GGTCTC 1 cut(s) 711
BsaJI CCNNGG 9 cut(s) 47, 48, 123, 157, 207, 407, 471, 1084, 1576
BsaWI WCCGGW 2 cut(s) 530, 1771
Bse1I ACTGG 8 cut(s) 843, 929, 940, 1028, 1345, 1409, 1596, 2045
BseAI TCCGGA 1 cut(s) 530
BseBI CCWGG 5 cut(s) 125, 473, 1085, 1578, 1815
BseDI CCNNGG 9 cut(s) 47, 48, 123, 157, 207, 407, 471, 1084, 1576
BseGI GGATG 2 cut(s) 527, 1881
BseMII CTCAG 1 cut(s) 843
BseNI ACTGG 8 cut(s) 843, 929, 940, 1028, 1345, 1409, 1596, 2045
BsePI GCGCGC 1 cut(s) 2027
BseRI GAGGAG 3 cut(s) 86, 128, 2053
BseSI GKGCMC 2 cut(s) 40, 472
BseXI GCAGC 5 cut(s) 599, 616, 1279, 1282, 1316
Bsh1236I CGCG 2 cut(s) 1380, 2029
BshFI GGCC 9 cut(s) 232, 260, 298, 484, 495, 1083, 1089, 1098, 2020
BshNI GGYRCC 3 cut(s) 35, 162, 1232
BsiHKAI GWGCWC 1 cut(s) 416
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 5 cut(s) 48, 267, 531, 1563, 1772
BsiWI CGTACG 1 cut(s) 86
BslFI GGGAC 2 cut(s) 101, 1464
BsmAI GTCTC 3 cut(s) 140, 711, 1317
BsmFI GGGAC 2 cut(s) 101, 1464
BsnI GGCC 9 cut(s) 232, 260, 298, 484, 495, 1083, 1089, 1098, 2020
Bso31I GGTCTC 1 cut(s) 711
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 4 cut(s) 40, 416, 472, 2045
Bsp13I TCCGGA 1 cut(s) 530
Bsp1407I TGTACA 1 cut(s) 1878
Bsp19I CCATGG 1 cut(s) 207
Bsp68I TCGCGA 1 cut(s) 1380
BspACI CCGC 9 cut(s) 119, 564, 719, 896, 1001, 1503, 1635, 1671, 1842
BspANI GGCC 9 cut(s) 232, 260, 298, 484, 495, 1083, 1089, 1098, 2020
BspCNI CTCAG 1 cut(s) 844
BspEI TCCGGA 1 cut(s) 530
BspFNI CGCG 2 cut(s) 1380, 2029
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 9 cut(s) 37, 164, 178, 323, 650, 1234, 1669, 2021, 2042
BspT107I GGYRCC 3 cut(s) 35, 162, 1232
BspTNI GGTCTC 1 cut(s) 711
BsrGI TGTACA 1 cut(s) 1878
BsrI ACTGG 8 cut(s) 843, 929, 940, 1028, 1345, 1409, 1596, 2045
BssECI CCNNGG 9 cut(s) 47, 48, 123, 157, 207, 407, 471, 1084, 1576
BssHII GCGCGC 1 cut(s) 2027
BssNI GRCGYC 2 cut(s) 163, 1477
BssT1I CCWWGG 2 cut(s) 157, 207
Bst2UI CCWGG 5 cut(s) 125, 473, 1085, 1578, 1815
Bst4CI ACNGT 4 cut(s) 411, 1242, 1433, 1720
BstACI GRCGYC 2 cut(s) 163, 1477
BstAPI GCANNNNNTGC 2 cut(s) 572, 1565
BstAUI TGTACA 1 cut(s) 1878
BstC8I GCNNGC 4 cut(s) 525, 1128, 1302, 2029
BstDEI CTNAG 3 cut(s) 852, 1357, 1370
BstDSI CCRYGG 2 cut(s) 207, 407
BstF5I GGATG 2 cut(s) 527, 1881
BstFNI CGCG 2 cut(s) 1380, 2029
BstH2I RGCGCY 2 cut(s) 166, 1470
BstHHI GCGC 7 cut(s) 6, 165, 1300, 1469, 1993, 2029, 2031
BstMAI GTCTC 3 cut(s) 140, 711, 1317
BstNI CCWGG 5 cut(s) 125, 473, 1085, 1578, 1815
BstNSI RCATGY 7 cut(s) 332, 401, 737, 1012, 1130, 1304, 1833
BstSCI CCNGG 9 cut(s) 46, 47, 123, 265, 471, 1083, 1561, 1576, 1813
BstSLI GKGCMC 2 cut(s) 40, 472
BstUI CGCG 2 cut(s) 1380, 2029
BstV1I GCAGC 5 cut(s) 599, 616, 1279, 1282, 1316
BstX2I RGATCY 8 cut(s) 52, 176, 315, 775, 786, 820, 925, 1107
BstXI CCANNNNNNTGG 2 cut(s) 214, 1315
BstYI RGATCY 8 cut(s) 52, 176, 315, 775, 786, 820, 925, 1107
BsuI GTATCC 3 cut(s) 1337, 1530, 1849
BsuRI GGCC 9 cut(s) 232, 260, 298, 484, 495, 1083, 1089, 1098, 2020
BtgI CCRYGG 2 cut(s) 207, 407
BtgZI GCGATG 3 cut(s) 268, 1361, 1395
BtsCI GGATG 2 cut(s) 527, 1881
BtsI GCAGTG 1 cut(s) 1635
BtsIMutI CAGTG 1 cut(s) 1635
BtuMI TCGCGA 1 cut(s) 1380
Cac8I GCNNGC 4 cut(s) 525, 1128, 1302, 2029
CaiI CAGNNNCTG 2 cut(s) 935, 1577
CciI TCATGA 1 cut(s) 799
CfoI GCGC 7 cut(s) 6, 165, 1300, 1469, 1993, 2029, 2031
Cfr9I CCCGGG 1 cut(s) 47
CseI GACGC 1 cut(s) 1466
Csp6I GTAC 8 cut(s) 87, 561, 731, 1233, 1367, 1685, 1827, 1879
CviQI GTAC 8 cut(s) 87, 561, 731, 1233, 1367, 1685, 1827, 1879
DdeI CTNAG 3 cut(s) 852, 1357, 1370
DinI GGCGCC 1 cut(s) 164
DraI TTTAAA 1 cut(s) 186
DraIII CACNNNGTG 1 cut(s) 1565
EaeI YGGCCR 1 cut(s) 482
EciI GGCGGA 1 cut(s) 1650
Eco130I CCWWGG 2 cut(s) 157, 207
Eco147I AGGCCT 1 cut(s) 1098
Eco24I GRGCYC 1 cut(s) 2045
Eco31I GGTCTC 1 cut(s) 711
Eco32I GATATC 1 cut(s) 376
Eco47I GGWCC 5 cut(s) 91, 461, 1017, 1070, 1811
Eco47III AGCGCT 1 cut(s) 1468
Eco88I CYCGRG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 296
EcoRII CCWGG 5 cut(s) 123, 471, 1083, 1576, 1813
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 157, 207
EcoT22I ATGCAT 1 cut(s) 1702
EcoT38I GRGCYC 1 cut(s) 2045
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
ErhI CCWWGG 2 cut(s) 157, 207
FalI AAGNNNNNCTT 2 cut(s) 1770, 1802
FaqI GGGAC 2 cut(s) 101, 1464
FauI CCCGC 3 cut(s) 126, 712, 994
FauNDI CATATG 2 cut(s) 1188, 1260
FbaI TGATCA 1 cut(s) 1951
Fnu4HI GCNGC 5 cut(s) 588, 630, 1293, 1296, 1305
FokI GGATG 2 cut(s) 514, 1888
FriOI GRGCYC 1 cut(s) 2045
Fsp4HI GCNGC 5 cut(s) 588, 630, 1293, 1296, 1305
FspBI CTAG 6 cut(s) 158, 465, 540, 695, 1148, 1217
FspI TGCGCA 2 cut(s) 1299, 1992
GlaI GCGC 7 cut(s) 5, 164, 1299, 1468, 1992, 2028, 2030
GluI GCNGC 5 cut(s) 588, 630, 1293, 1296, 1305
GsuI CTGGAG 1 cut(s) 789
HaeII RGCGCY 2 cut(s) 166, 1470
HaeIII GGCC 9 cut(s) 232, 260, 298, 484, 495, 1083, 1089, 1098, 2020
HapII CCGG 5 cut(s) 48, 267, 531, 1563, 1772
HgaI GACGC 1 cut(s) 1466
HhaI GCGC 7 cut(s) 6, 165, 1300, 1469, 1993, 2029, 2031
Hin1I GRCGYC 2 cut(s) 163, 1477
Hin6I GCGC 7 cut(s) 4, 163, 1298, 1467, 1991, 2027, 2029
HinP1I GCGC 7 cut(s) 4, 163, 1298, 1467, 1991, 2027, 2029
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 4 cut(s) 662, 673, 814, 1708
HpaII CCGG 5 cut(s) 48, 267, 531, 1563, 1772
HphI GGTGA 3 cut(s) 524, 668, 1175
Hpy166II GTNNAC 3 cut(s) 15, 1251, 1367
Hpy188I TCNGA 2 cut(s) 1498, 1930
Hpy8I GTNNAC 3 cut(s) 15, 1251, 1367
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 3 cut(s) 17, 1812, 2048
HpyCH4III ACNGT 4 cut(s) 411, 1242, 1433, 1720
HpyCH4IV ACGT 3 cut(s) 23, 1118, 1932
HpyF3I CTNAG 3 cut(s) 852, 1357, 1370
HpySE526I ACGT 3 cut(s) 23, 1118, 1932
Hsp92I GRCGYC 2 cut(s) 163, 1477
HspAI GCGC 7 cut(s) 4, 163, 1298, 1467, 1991, 2027, 2029
KasI GGCGCC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 530
KpnI GGTACC 1 cut(s) 1236
Ksp22I TGATCA 1 cut(s) 1951
LmnI GCTCC 3 cut(s) 637, 857, 2040
Lsp1109I GCAGC 5 cut(s) 599, 616, 1279, 1282, 1316
LweI GCATC 6 cut(s) 325, 536, 733, 1188, 1372, 1384
MaeI CTAG 6 cut(s) 158, 465, 540, 695, 1148, 1217
MaeII ACGT 3 cut(s) 23, 1118, 1932
MaeIII GTNAC 5 cut(s) 931, 1004, 1650, 1714, 1963
MboII GAAGA 2 cut(s) 121, 1894
MfeI CAATTG 2 cut(s) 224, 567
MflI RGATCY 8 cut(s) 52, 176, 315, 775, 786, 820, 925, 1107
MhlI GDGCHC 4 cut(s) 40, 416, 472, 2045
MlsI TGGCCA 1 cut(s) 484
MluNI TGGCCA 1 cut(s) 484
Mly113I GGCGCC 1 cut(s) 163
MmeI TCCRAC 2 cut(s) 1358, 1895
Mox20I TGGCCA 1 cut(s) 484
Mph1103I ATGCAT 1 cut(s) 1702
MroI TCCGGA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 484
MseI TTAA 9 cut(s) 59, 185, 440, 593, 750, 872, 1143, 1166, 2010
MslI CAYNNNNRTG 6 cut(s) 212, 333, 360, 1313, 1380, 1695
Msp20I TGGCCA 1 cut(s) 484
MspI CCGG 5 cut(s) 48, 267, 531, 1563, 1772
MspR9I CCNGG 9 cut(s) 48, 49, 125, 267, 473, 1085, 1563, 1578, 1815
MunI CAATTG 2 cut(s) 224, 567
MvaI CCWGG 5 cut(s) 125, 473, 1085, 1578, 1815
MvnI CGCG 2 cut(s) 1380, 2029
NarI GGCGCC 1 cut(s) 163
NciI CCSGG 4 cut(s) 48, 49, 267, 1563
NcoI CCATGG 1 cut(s) 207
NdeI CATATG 2 cut(s) 1188, 1260
NlaIV GGNNCC 9 cut(s) 37, 164, 178, 323, 650, 1234, 1669, 2021, 2042
NmuCI GTSAC 2 cut(s) 1004, 1714
NruI TCGCGA 1 cut(s) 1380
NsbI TGCGCA 2 cut(s) 1299, 1992
NsiI ATGCAT 1 cut(s) 1702
NspI RCATGY 7 cut(s) 332, 401, 737, 1012, 1130, 1304, 1833
PaeI GCATGC 2 cut(s) 1130, 1304
PagI TCATGA 1 cut(s) 799
PauI GCGCGC 1 cut(s) 2027
PceI AGGCCT 1 cut(s) 1098
PciI ACATGT 2 cut(s) 733, 1008
PcsI WCGNNNNNNNCGW 2 cut(s) 82, 1759
PfeI GAWTC 4 cut(s) 662, 673, 814, 1708
Pfl23II CGTACG 1 cut(s) 86
PflFI GACNNNGTC 1 cut(s) 267
PflMI CCANNNNNTGG 2 cut(s) 935, 1340
PkrI GCNGC 5 cut(s) 589, 631, 1294, 1297, 1306
PluTI GGCGCC 1 cut(s) 166
PscI ACATGT 2 cut(s) 733, 1008
PshBI ATTAAT 1 cut(s) 2010
Psp6I CCWGG 5 cut(s) 123, 471, 1083, 1576, 1813
PspGI CCWGG 5 cut(s) 123, 471, 1083, 1576, 1813
PspLI CGTACG 1 cut(s) 86
PspN4I GGNNCC 9 cut(s) 37, 164, 178, 323, 650, 1234, 1669, 2021, 2042
PstNI CAGNNNCTG 2 cut(s) 935, 1577
PsuI RGATCY 8 cut(s) 52, 176, 315, 775, 786, 820, 925, 1107
PsyI GACNNNGTC 1 cut(s) 267
PteI GCGCGC 1 cut(s) 2027
RruI TCGCGA 1 cut(s) 1380
RsaI GTAC 8 cut(s) 88, 562, 732, 1234, 1368, 1686, 1828, 1880
RsaNI GTAC 8 cut(s) 87, 561, 731, 1233, 1367, 1685, 1827, 1879
RseI CAYNNNNRTG 6 cut(s) 212, 333, 360, 1313, 1380, 1695
SaqAI TTAA 9 cut(s) 59, 185, 440, 593, 750, 872, 1143, 1166, 2010
SatI GCNGC 5 cut(s) 588, 630, 1293, 1296, 1305
ScrFI CCNGG 9 cut(s) 48, 49, 125, 267, 473, 1085, 1563, 1578, 1815
SduI GDGCHC 4 cut(s) 40, 416, 472, 2045
SfaNI GCATC 6 cut(s) 325, 536, 733, 1188, 1372, 1384
SfiI GGCCNNNNNGGCC 1 cut(s) 1095
SfoI GGCGCC 1 cut(s) 164
SinI GGWCC 5 cut(s) 91, 461, 1017, 1070, 1811
SmaI CCCGGG 1 cut(s) 49
SmiMI CAYNNNNRTG 6 cut(s) 212, 333, 360, 1313, 1380, 1695
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
SpeI ACTAGT 1 cut(s) 539
SphI GCATGC 2 cut(s) 1130, 1304
SseBI AGGCCT 1 cut(s) 1098
SsiI CCGC 9 cut(s) 119, 564, 719, 896, 1001, 1503, 1635, 1671, 1842
SspDI GGCGCC 1 cut(s) 162
SspI AATATT 1 cut(s) 504
SspMI CTAG 6 cut(s) 158, 465, 540, 695, 1148, 1217
StuI AGGCCT 1 cut(s) 1098
StyD4I CCNGG 9 cut(s) 46, 47, 123, 265, 471, 1083, 1561, 1576, 1813
StyI CCWWGG 2 cut(s) 157, 207
TaaI ACNGT 4 cut(s) 411, 1242, 1433, 1720
TaiI ACGT 3 cut(s) 26, 1121, 1935
TaqI TCGA 5 cut(s) 20, 147, 249, 348, 1196
TatI WGTACW 3 cut(s) 730, 1366, 1878
TfiI GAWTC 4 cut(s) 662, 673, 814, 1708
Tru1I TTAA 9 cut(s) 59, 185, 440, 593, 750, 872, 1143, 1166, 2010
Tru9I TTAA 9 cut(s) 59, 185, 440, 593, 750, 872, 1143, 1166, 2010
TscAI CASTG 1 cut(s) 1635
TseFI GTSAC 2 cut(s) 1004, 1714
TseI GCWGC 5 cut(s) 587, 629, 1292, 1295, 1304
Tsp45I GTSAC 2 cut(s) 1004, 1714
TspDTI ATGAA 6 cut(s) 444, 605, 816, 1439, 1685, 1700
TspGWI ACGGA 1 cut(s) 104
TspMI CCCGGG 1 cut(s) 47
TspRI CASTG 1 cut(s) 1635
Tth111I GACNNNGTC 1 cut(s) 267
Van91I CCANNNNNTGG 2 cut(s) 935, 1340
VpaK11BI GGWCC 5 cut(s) 91, 461, 1017, 1070, 1811
VspI ATTAAT 1 cut(s) 2010
XapI RAATTY 2 cut(s) 803, 899
XceI RCATGY 7 cut(s) 332, 401, 737, 1012, 1130, 1304, 1833
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XmaI CCCGGG 1 cut(s) 47
XmaJI CCTAGG 1 cut(s) 157
XspI CTAG 6 cut(s) 158, 465, 540, 695, 1148, 1217
Zsp2I ATGCAT 1 cut(s) 1702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.