MD11G1112200.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008874
Physical Location & Seq
Forward (+)
9930075 .. 9931125
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1112200.v1.1.491

Sequence Viewer

Length: 900 bp
ATGATGACTGGTGAAGAGTTTTTAGTTACCAAATGTAATAGAACTGCACAAGGTTCTAGTGCTTTTGTTGGAACACATCTTAAGAGGGTTGCTGAACAACCGCAAGCGGTGACTGCCAGAGCTCAACTGTTAAAGAGAAGAAGAGCTCAATCTTCTGTCGATCCATCTGCTCCAATGTTGTCCCTAAATGTTTCAACTGTCCCAAATTCATCCAAGGCCTCTTCTTTCACAGTCCCAGATCCTACAGCTCCATCCCTCCCATGGGAAGCGAAAAGTACTACATCACTCTCACCCCTATTGCAGATTTCTCACACTCTCCCAGCCCAAGGAAGAAGTACTCCATTATTTCCACCCCTGTCACAGACTACGTTTGCCCTCCCAACCCCAGTCAGACGTACTGCAGTACTCCCAGCCTTATCCCAGATTGCTTCCTCTCTGCCATCCCGAGTGAAAACTACTAAATTGCATCCGCCTCTATCCTTGACTGCTCCATGTCTCAGACCCAAAGCCAAAACTACTACATCGCACCCGGCCCTATCCCAGAATGCTCCATCTTTCCTACCTCTACCCCAAATTGCTCCTCGACTCGCATCACACCCGACCCAGTCCTGGAATGCTCCCTCTCTCCGACCCCAGGTCCGAATTGTACCCCCACTCCCATCCCAACCTTGGGCTGCTCCACCAGTCCCACAGATATCTCAGAATGCTTCACCACTCCCACCAAAATCTCAGTCTGCTTCATCACTCTCTCCCAGACTGCTCAACCTCCCCTTCCACCACCCCAGATTGCTCCCTCTCTCCTACCCAAACGCCAGACTGGTCCACCTTCCCCACCCCAACCCCAGACTGCTGCACCCCCTCTTCCACCACAATCCCGGACTGCTTCAGCTGTACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

32.31

Weight (kDa)

11.82

Isoelectric Point (pI)

68.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 101, 107, 470
AclWI GGATC 2 cut(s) 155, 233
AcsI RAATTY 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 869
AfaI GTAC 6 cut(s) 277, 337, 397, 405, 648, 893
AfiI CCNNNNNNNGG 7 cut(s) 261, 262, 326, 609, 634, 669, 670
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 1 cut(s) 195
AhdI GACNNNNNGTC 1 cut(s) 635
AjnI CCWGG 2 cut(s) 608, 633
AluBI AGCT 4 cut(s) 122, 146, 248, 889
AluI AGCT 4 cut(s) 122, 146, 248, 889
Alw21I GWGCWC 2 cut(s) 124, 148
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 2 cut(s) 155, 233
Ama87I CYCGRG 1 cut(s) 444
AoxI GGCC 2 cut(s) 216, 531
ApeKI GCWGC 2 cut(s) 674, 850
ApoI RAATTY 1 cut(s) 205
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
AspS9I GGNCC 3 cut(s) 532, 637, 820
AsuC2I CCSGG 2 cut(s) 530, 876
AsuHPI GGTGA 4 cut(s) 23, 121, 282, 702
AvaI CYCGRG 1 cut(s) 444
AvaII GGWCC 2 cut(s) 637, 820
BanII GRGCYC 2 cut(s) 124, 148
Bbv12I GWGCWC 2 cut(s) 124, 148
BbvI GCAGC 2 cut(s) 661, 837
BccI CCATC 5 cut(s) 172, 259, 448, 559, 667
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 2 cut(s) 610, 635
BcnI CCSGG 2 cut(s) 530, 876
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 2 cut(s) 243, 399
BfrI CTTAAG 1 cut(s) 80
BisI GCNGC 2 cut(s) 675, 851
BlsI GCNGC 2 cut(s) 676, 852
BmcAI AGTACT 3 cut(s) 277, 337, 405
Bme1390I CCNGG 4 cut(s) 530, 610, 635, 876
Bme18I GGWCC 2 cut(s) 637, 820
BmeRI GACNNNNNGTC 1 cut(s) 635
BmeT110I CYCGRG 1 cut(s) 444
BmgT120I GGNCC 3 cut(s) 532, 637, 820
BmrFI CCNGG 4 cut(s) 530, 610, 635, 876
BmrI ACTGGG 2 cut(s) 380, 598
BmsI GCATC 2 cut(s) 475, 599
BmuI ACTGGG 2 cut(s) 380, 598
BpuMI CCSGG 2 cut(s) 530, 876
BsaJI CCNNGG 5 cut(s) 213, 260, 325, 633, 668
BsaXI ACNNNNNCTCC 2 cut(s) 639, 669
Bsc4I CCNNNNNNNGG 7 cut(s) 261, 262, 326, 609, 634, 669, 670
Bse1I ACTGG 5 cut(s) 13, 386, 604, 683, 822
BseBI CCWGG 2 cut(s) 610, 635
BseDI CCNNGG 5 cut(s) 213, 260, 325, 633, 668
BseGI GGATG 5 cut(s) 209, 251, 440, 466, 659
BseLI CCNNNNNNNGG 7 cut(s) 261, 262, 326, 609, 634, 669, 670
BseMII CTCAG 3 cut(s) 511, 713, 743
BseNI ACTGG 5 cut(s) 13, 386, 604, 683, 822
BseRI GAGGAG 1 cut(s) 570
BseXI GCAGC 2 cut(s) 661, 837
BseYI CCCAGC 2 cut(s) 319, 409
BsgI GTGCAG 2 cut(s) 30, 836
BshFI GGCC 2 cut(s) 218, 533
BsiHKAI GWGCWC 2 cut(s) 124, 148
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 2 cut(s) 530, 876
BslFI GGGAC 4 cut(s) 166, 185, 218, 671
BslI CCNNNNNNNGG 7 cut(s) 261, 262, 326, 609, 634, 669, 670
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 4 cut(s) 166, 185, 218, 671
BsmI GAATGC 3 cut(s) 550, 619, 709
BsnI GGCC 2 cut(s) 218, 533
BsoBI CYCGRG 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 124, 148
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 2 cut(s) 160, 238
Bsp19I CCATGG 1 cut(s) 260
BspACI CCGC 3 cut(s) 101, 107, 470
BspANI GGCC 2 cut(s) 218, 533
BspCNI CTCAG 3 cut(s) 510, 712, 742
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 2 cut(s) 155, 233
BspQI GCTCTTC 1 cut(s) 136
BspTI CTTAAG 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 5 cut(s) 13, 386, 604, 683, 822
BssECI CCNNGG 5 cut(s) 213, 260, 325, 633, 668
BssMI GATC 2 cut(s) 160, 238
BssT1I CCWWGG 4 cut(s) 213, 260, 325, 668
Bst2UI CCWGG 2 cut(s) 610, 635
Bst4CI ACNGT 3 cut(s) 129, 199, 232
Bst6I CTCTTC 4 cut(s) 9, 136, 226, 866
BstAFI CTTAAG 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 891
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 3 cut(s) 497, 699, 729
BstDSI CCRYGG 1 cut(s) 260
BstF5I GGATG 5 cut(s) 209, 251, 440, 466, 659
BstKTI GATC 2 cut(s) 163, 241
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 2 cut(s) 160, 238
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 2 cut(s) 610, 635
BstSCI CCNGG 4 cut(s) 528, 608, 633, 874
BstSFI CTRYAG 2 cut(s) 243, 399
BstV1I GCAGC 2 cut(s) 661, 837
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 2 cut(s) 218, 533
BtgI CCRYGG 1 cut(s) 260
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 5 cut(s) 209, 251, 440, 466, 659
Cac8I GCNNGC 1 cut(s) 105
Cfr13I GGNCC 3 cut(s) 532, 637, 820
Csp6I GTAC 6 cut(s) 276, 336, 396, 404, 647, 892
CviAII CATG 2 cut(s) 261, 492
CviQI GTAC 6 cut(s) 276, 336, 396, 404, 647, 892
DdeI CTNAG 3 cut(s) 497, 699, 729
DpnI GATC 2 cut(s) 162, 240
DpnII GATC 2 cut(s) 160, 238
DriI GACNNNNNGTC 1 cut(s) 635
Eam1104I CTCTTC 4 cut(s) 9, 136, 226, 866
Eam1105I GACNNNNNGTC 1 cut(s) 635
EarI CTCTTC 4 cut(s) 9, 136, 226, 866
EciI GGCGGA 1 cut(s) 459
Ecl136II GAGCTC 2 cut(s) 122, 146
Eco130I CCWWGG 4 cut(s) 213, 260, 325, 668
Eco147I AGGCCT 1 cut(s) 218
Eco24I GRGCYC 2 cut(s) 124, 148
Eco32I GATATC 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 637, 820
Eco53kI GAGCTC 2 cut(s) 122, 146
Eco57I CTGAAG 1 cut(s) 869
Eco88I CYCGRG 1 cut(s) 444
EcoICRI GAGCTC 2 cut(s) 122, 146
EcoRII CCWGG 2 cut(s) 608, 633
EcoRV GATATC 1 cut(s) 696
EcoT14I CCWWGG 4 cut(s) 213, 260, 325, 668
EcoT38I GRGCYC 2 cut(s) 124, 148
ErhI CCWWGG 4 cut(s) 213, 260, 325, 668
FaeI CATG 2 cut(s) 264, 495
FaiI YATR 4 cut(s) 262, 493, 896, 898
FaqI GGGAC 4 cut(s) 166, 185, 218, 671
FatI CATG 2 cut(s) 260, 491
Fnu4HI GCNGC 2 cut(s) 675, 851
FokI GGATG 5 cut(s) 196, 238, 427, 453, 646
FriOI GRGCYC 2 cut(s) 124, 148
Fsp4HI GCNGC 2 cut(s) 675, 851
FspBI CTAG 1 cut(s) 57
GluI GCNGC 2 cut(s) 675, 851
GsaI CCCAGC 2 cut(s) 323, 413
HaeIII GGCC 2 cut(s) 218, 533
HapII CCGG 2 cut(s) 530, 876
Hin1II CATG 2 cut(s) 264, 495
HinfI GANTC 1 cut(s) 585
HpaII CCGG 2 cut(s) 530, 876
HphI GGTGA 4 cut(s) 23, 121, 282, 702
Hpy166II GTNNAC 1 cut(s) 823
Hpy188I TCNGA 5 cut(s) 392, 500, 629, 641, 702
Hpy188III TCNNGA 1 cut(s) 444
Hpy8I GTNNAC 1 cut(s) 823
HpyAV CCTTC 2 cut(s) 781, 836
HpyCH4III ACNGT 3 cut(s) 129, 199, 232
HpyCH4IV ACGT 2 cut(s) 368, 394
HpyCH4V TGCA 5 cut(s) 47, 301, 401, 466, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 3 cut(s) 497, 699, 729
HpySE526I ACGT 2 cut(s) 368, 394
Hsp92II CATG 2 cut(s) 264, 495
Kzo9I GATC 2 cut(s) 160, 238
LguI GCTCTTC 1 cut(s) 136
LmnI GCTCC 8 cut(s) 175, 253, 493, 553, 583, 622, 682, 795
Lsp1109I GCAGC 2 cut(s) 661, 837
LweI GCATC 2 cut(s) 475, 599
MaeI CTAG 1 cut(s) 57
MaeII ACGT 2 cut(s) 368, 394
MaeIII GTNAC 3 cut(s) 25, 109, 357
MalI GATC 2 cut(s) 162, 240
MboI GATC 2 cut(s) 160, 238
MboII GAAGA 7 cut(s) 26, 144, 150, 153, 213, 342, 853
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 124, 148
MluCI AATT 4 cut(s) 205, 461, 573, 642
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 2 cut(s) 49, 652
MseI TTAA 2 cut(s) 81, 131
MspA1I CMGCKG 1 cut(s) 889
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 2 cut(s) 530, 876
MspR9I CCNGG 4 cut(s) 530, 610, 635, 876
Mva1269I GAATGC 3 cut(s) 550, 619, 709
MvaI CCWGG 2 cut(s) 610, 635
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 2 cut(s) 530, 876
NcoI CCATGG 1 cut(s) 260
NdeII GATC 2 cut(s) 160, 238
NlaIII CATG 2 cut(s) 264, 495
NmuCI GTSAC 2 cut(s) 109, 357
PceI AGGCCT 1 cut(s) 218
PciSI GCTCTTC 1 cut(s) 136
PctI GAATGC 3 cut(s) 550, 619, 709
PflFI GACNNNGTC 1 cut(s) 604
PfoI TCCNGGA 2 cut(s) 608, 874
PkrI GCNGC 2 cut(s) 676, 852
PleI GAGTC 1 cut(s) 579
PpsI GAGTC 1 cut(s) 579
Psp124BI GAGCTC 2 cut(s) 124, 148
Psp6I CCWGG 2 cut(s) 608, 633
PspFI CCCAGC 2 cut(s) 319, 409
PspGI CCWGG 2 cut(s) 608, 633
PspPI GGNCC 3 cut(s) 532, 637, 820
PstI CTGCAG 1 cut(s) 403
PsuI RGATCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 889
RsaI GTAC 6 cut(s) 277, 337, 397, 405, 648, 893
RsaNI GTAC 6 cut(s) 276, 336, 396, 404, 647, 892
SacI GAGCTC 2 cut(s) 124, 148
SapI GCTCTTC 1 cut(s) 136
SaqAI TTAA 2 cut(s) 81, 131
SatI GCNGC 2 cut(s) 675, 851
Sau3AI GATC 2 cut(s) 160, 238
Sau96I GGNCC 3 cut(s) 532, 637, 820
ScaI AGTACT 3 cut(s) 277, 337, 405
SchI GAGTC 1 cut(s) 579
ScrFI CCNGG 4 cut(s) 530, 610, 635, 876
SduI GDGCHC 2 cut(s) 124, 148
SfaNI GCATC 2 cut(s) 475, 599
SfcI CTRYAG 2 cut(s) 243, 399
SinI GGWCC 2 cut(s) 637, 820
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 4 cut(s) 205, 461, 573, 642
SseBI AGGCCT 1 cut(s) 218
SsiI CCGC 3 cut(s) 101, 107, 470
SspMI CTAG 1 cut(s) 57
SstI GAGCTC 2 cut(s) 124, 148
StuI AGGCCT 1 cut(s) 218
StyD4I CCNGG 4 cut(s) 528, 608, 633, 874
StyI CCWWGG 4 cut(s) 213, 260, 325, 668
TaaI ACNGT 3 cut(s) 129, 199, 232
TaiI ACGT 2 cut(s) 371, 397
TaqI TCGA 2 cut(s) 159, 583
TasI AATT 4 cut(s) 205, 461, 573, 642
TatI WGTACW 4 cut(s) 275, 335, 403, 891
Tru1I TTAA 2 cut(s) 81, 131
Tru9I TTAA 2 cut(s) 81, 131
TseFI GTSAC 2 cut(s) 109, 357
TseI GCWGC 2 cut(s) 674, 850
Tsp45I GTSAC 2 cut(s) 109, 357
TspDTI ATGAA 2 cut(s) 198, 729
Tth111I GACNNNGTC 1 cut(s) 604
Vha464I CTTAAG 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 637, 820
XapI RAATTY 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 2 cut(s) 258, 666
XspI CTAG 1 cut(s) 57
ZrmI AGTACT 3 cut(s) 277, 337, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.