Rorug02G0303800

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008874
Physical Location & Seq
Reverse (-)
34017636 .. 34022189
4554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0303800.1

Sequence Viewer

Length: 675 bp
ATGGAGGGCAAACACCTGTTTTTGAAGCTGGCAGAGTACGGGGTGCGGGTCAATCTTAATGATTCTGATCGGCAAAAGGCTGAGGAAATTTCCCACCTCTTCATCAAAAGATTTCCTGATGAGTCGGTACAGAAATCGATAATCTTTGCCATCGCCAGGGTTACGAGAAACCGCCTTAGACCTAAGGTGATAGACGAGGAGCTTATTCGAAAATATATTGAAAACACGAAGAAGCGGAAGAAGCCGGAATTCGAGATGATGTCGGAGGATGAGGAGGAGGAGGAGGAGACGCTGTTGGAGGAGGGGGAAGAAGAACCGACAGAAGAAGACAGGAAGGTGTGGTTGGTACTTTGCAGGAGACTGGGATTCAGAATGAAACGAGGAGAAGATGTCAAGATGCTCAGGCTGATGATTCCTTTCTTAGCCCAATGTTCTGGACTTTATCCACCAAAAGGATCCACAAGACGTGGCTGCATCAGAATATTCATGTACCAGTTCCTAAGATATATCGATATGTATTGCCTTCGCGACCTGAACGACGTGGGGATGTTGCTCGACCTCATTGAGGCTCTCAAGTCACGTCGAGCAAATCCAGAAAATAAGTTAAAGCAAAATATCGTACCAACTAAAGAAGAAGGGATTGCTGCCCCCATGTACCTCTTAAAACTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

26.59

Weight (kDa)

7.61

Isoelectric Point (pI)

64.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 528
AciI CCGC 3 cut(s) 46, 172, 235
AclWI GGATC 2 cut(s) 450, 463
AcsI RAATTY 2 cut(s) 87, 248
AfaI GTAC 6 cut(s) 38, 129, 348, 491, 621, 656
AfiI CCNNNNNNNGG 3 cut(s) 156, 452, 565
AgsI TTSAA 2 cut(s) 25, 221
AjiI CACGTC 3 cut(s) 467, 541, 581
AjnI CCWGG 1 cut(s) 155
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 2 cut(s) 28, 202
AluI AGCT 2 cut(s) 28, 202
Alw26I GTCTC 2 cut(s) 281, 352
AlwI GGATC 2 cut(s) 450, 463
ApeKI GCWGC 2 cut(s) 471, 644
ApoI RAATTY 2 cut(s) 87, 248
AsuHPI GGTGA 1 cut(s) 199
AsuII TTCGAA 1 cut(s) 208
AxyI CCTNAGG 1 cut(s) 183
BamHI GGATCC 1 cut(s) 455
BbsI GAAGAC 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 458, 631
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 281, 352
BfaI CTAG 1 cut(s) 668
BisI GCNGC 2 cut(s) 472, 645
BlsI GCNGC 2 cut(s) 473, 646
Bme1390I CCNGG 1 cut(s) 157
BmgBI CACGTC 3 cut(s) 467, 541, 581
BmiI GGNNCC 1 cut(s) 457
BmrFI CCNGG 1 cut(s) 157
BmrI ACTGGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 387, 483
BmuI ACTGGG 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 333
Bpu10I CCTNAGC 2 cut(s) 81, 401
Bpu14I TTCGAA 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 557
Bsa29I ATCGAT 2 cut(s) 137, 510
BsaBI GATNNNNATC 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 156
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 375, 405
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 452, 565
Bse1I ACTGG 2 cut(s) 366, 493
Bse21I CCTNAGG 1 cut(s) 183
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 1 cut(s) 157
BseCI ATCGAT 2 cut(s) 137, 510
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 2 cut(s) 274, 552
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 3 cut(s) 156, 452, 565
BseMII CTCAG 2 cut(s) 72, 415
BseNI ACTGG 2 cut(s) 366, 493
BseRI GAGGAG 8 cut(s) 212, 287, 290, 293, 296, 299, 314, 396
BseXI GCAGC 2 cut(s) 458, 631
Bsh1236I CGCG 1 cut(s) 528
BshVI ATCGAT 2 cut(s) 137, 510
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 3 cut(s) 156, 452, 565
BsmAI GTCTC 2 cut(s) 281, 352
BsmBI CGTCTC 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 208
Bsp143I GATC 2 cut(s) 67, 455
Bsp68I TCGCGA 1 cut(s) 528
BspACI CCGC 3 cut(s) 46, 172, 235
BspCNI CTCAG 2 cut(s) 73, 414
BspDI ATCGAT 2 cut(s) 137, 510
BspFNI CGCG 1 cut(s) 528
BspLI GGNNCC 1 cut(s) 457
BspPI GGATC 2 cut(s) 450, 463
BspT104I TTCGAA 1 cut(s) 208
BsrI ACTGG 2 cut(s) 366, 493
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 2 cut(s) 67, 455
Bst2UI CCWGG 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 104
BstBI TTCGAA 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 6 cut(s) 81, 176, 183, 401, 421, 500
BstENI CCTNNNNNAGG 1 cut(s) 563
BstF5I GGATG 2 cut(s) 274, 552
BstFNI CGCG 1 cut(s) 528
BstKTI GATC 2 cut(s) 70, 458
BstMAI GTCTC 2 cut(s) 281, 352
BstMBI GATC 2 cut(s) 67, 455
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 155
BstUI CGCG 1 cut(s) 528
BstV1I GCAGC 2 cut(s) 458, 631
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 455
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 1 cut(s) 455
Bsu15I ATCGAT 2 cut(s) 137, 510
Bsu36I CCTNAGG 1 cut(s) 183
BsuTUI ATCGAT 2 cut(s) 137, 510
BtgZI GCGATG 1 cut(s) 136
BtrI CACGTC 3 cut(s) 467, 541, 581
BtsCI GGATG 2 cut(s) 274, 552
BtuMI TCGCGA 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 30
ClaI ATCGAT 2 cut(s) 137, 510
CseI GACGC 1 cut(s) 298
Csp6I GTAC 6 cut(s) 37, 128, 347, 490, 620, 655
CviAII CATG 2 cut(s) 487, 652
CviJI RGCY 8 cut(s) 28, 80, 202, 244, 406, 425, 471, 569
CviKI_1 RGCY 8 cut(s) 28, 80, 202, 244, 406, 425, 471, 569
CviQI GTAC 6 cut(s) 37, 128, 347, 490, 620, 655
DdeI CTNAG 6 cut(s) 81, 176, 183, 401, 421, 500
DpnI GATC 2 cut(s) 69, 457
DpnII GATC 2 cut(s) 67, 455
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 183
EcoNI CCTNNNNNAGG 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 155
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 2 cut(s) 490, 655
FaiI YATR 5 cut(s) 216, 488, 507, 515, 653
FatI CATG 2 cut(s) 486, 651
FauI CCCGC 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 472, 645
FokI GGATG 2 cut(s) 281, 559
Fsp4HI GCNGC 2 cut(s) 472, 645
FspBI CTAG 1 cut(s) 668
GluI GCNGC 2 cut(s) 472, 645
HapII CCGG 1 cut(s) 245
HgaI GACGC 1 cut(s) 298
Hin1II CATG 2 cut(s) 490, 655
HinfI GANTC 4 cut(s) 62, 122, 366, 412
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 199
Hpy188I TCNGA 4 cut(s) 67, 265, 371, 479
Hpy188III TCNNGA 6 cut(s) 116, 253, 394, 435, 527, 593
Hpy99I CGWCG 2 cut(s) 542, 585
HpyAV CCTTC 3 cut(s) 328, 533, 629
HpyCH4IV ACGT 3 cut(s) 466, 540, 580
HpyCH4V TGCA 2 cut(s) 354, 474
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 6 cut(s) 81, 176, 183, 401, 421, 500
HpySE526I ACGT 3 cut(s) 466, 540, 580
Hsp92II CATG 2 cut(s) 490, 655
Kzo9I GATC 2 cut(s) 67, 455
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 2 cut(s) 458, 631
LweI GCATC 2 cut(s) 387, 483
MaeI CTAG 1 cut(s) 668
MaeII ACGT 3 cut(s) 466, 540, 580
MaeIII GTNAC 2 cut(s) 160, 576
MalI GATC 2 cut(s) 69, 457
MboI GATC 2 cut(s) 67, 455
MboII GAAGA 9 cut(s) 91, 241, 250, 320, 323, 335, 338, 398, 644
MflI RGATCY 1 cut(s) 455
MluCI AATT 2 cut(s) 87, 248
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 243, 276
MseI TTAA 3 cut(s) 57, 605, 662
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 157
MvaI CCWGG 1 cut(s) 157
MvnI CGCG 1 cut(s) 528
MwoI GCNNNNNNNGC 1 cut(s) 241
NdeII GATC 2 cut(s) 67, 455
NlaIII CATG 2 cut(s) 490, 655
NlaIV GGNNCC 1 cut(s) 457
NmuCI GTSAC 1 cut(s) 576
NruI TCGCGA 1 cut(s) 528
NspV TTCGAA 1 cut(s) 208
PfeI GAWTC 3 cut(s) 62, 366, 412
PkrI GCNGC 2 cut(s) 473, 646
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 457
PsuI RGATCY 1 cut(s) 455
RruI TCGCGA 1 cut(s) 528
RsaI GTAC 6 cut(s) 38, 129, 348, 491, 621, 656
RsaNI GTAC 6 cut(s) 37, 128, 347, 490, 620, 655
SaqAI TTAA 3 cut(s) 57, 605, 662
SatI GCNGC 2 cut(s) 472, 645
Sau3AI GATC 2 cut(s) 67, 455
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 157
SfaNI GCATC 2 cut(s) 387, 483
SfuI TTCGAA 1 cut(s) 208
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse9I AATT 2 cut(s) 87, 248
SsiI CCGC 3 cut(s) 46, 172, 235
SspI AATATT 1 cut(s) 483
SspMI CTAG 1 cut(s) 668
StyD4I CCNGG 1 cut(s) 155
TaiI ACGT 3 cut(s) 469, 543, 583
TaqI TCGA 6 cut(s) 137, 208, 252, 510, 555, 583
TasI AATT 2 cut(s) 87, 248
TfiI GAWTC 3 cut(s) 62, 366, 412
Tru1I TTAA 3 cut(s) 57, 605, 662
Tru9I TTAA 3 cut(s) 57, 605, 662
TseFI GTSAC 1 cut(s) 576
TseI GCWGC 2 cut(s) 471, 644
Tsp45I GTSAC 1 cut(s) 576
TspDTI ATGAA 3 cut(s) 91, 389, 475
XagI CCTNNNNNAGG 1 cut(s) 563
XapI RAATTY 2 cut(s) 87, 248
XspI CTAG 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.