MD11G1182800.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003248
Physical Location & Seq
Reverse (-)
23051708 .. 23057008
5301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1182800.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGCTTGAAGCAAATAAGTTGCATTTCCGTCCAATTTTCCCGGAAAAAGAAAGTGGATCAATTTCATCTAAATTCTTGAAGGGTTGTTTCTCCTGTGGTGAAGACCCAAGTCAAGTTCAGGAAGAGGCTCCTCAAACTTCTGTGAAGTACTTAACTTGGCATAGGACGACATCATCTCGATCCTCTTCGTCAAGGAATCCTTTGGGAGCAAACTTAAAAGATGATATGGATGATCTTACTGGTCACCTCTTTGACAACTTCTGCATGAATTCAGGCAGTCATGCCTCAACCTTGGATAGGCTATATGCGTGGGAGAGGAAGCTTTATGATGAAGTCAAGGCCAGTGAGATGGTTAGAAGGGATTATGGCTTGAAGTGTAAGATTTTAAGGCAGCTAGAATCAAAAGGACAGAGTTCTCAAATAACTGATAAAACTCGTGCTGTTGTCAAGGATCTACATTCTAGAATCGGGGTTGCTATTCACAGAATTGACTCAATATCGAAGAGGATTGAGGATTTACGAGACAAAGAGCTCCAGCCTCAACTTGAAGAGTTAATAGAAGGGTTAAGGCGGATGTGGGAGGGGTTGGTTGTATGTCACAAGCTTCAGTTTCACATAATCTCAATAGCTTACAACAACAGCAACACCAAAATCTCCGTACAGTCAGAATCACGTCGTCAGATTACGATCCAGCTTAAAGACGAACTCAGCTCCCTATCATCAAGTTTTATGAAGTGGAATGGTGCTCAAAAGTCCTACTTGCAGTCTATAGACGGATGGCTTTTAAAATGCATCTCTTTTCCACAGAAGTCTTCCAAAAAGAAAAGGAGGCCGTACGAAATCCCCTATAGGAACTATGGCCCTCCAATATATGTCACCTGTGGTGACTGGTTGGAAAAGCTTGGCAGTTTACCTGCCAAAGAAGTGGAAGATTCTATAAAGACTTTAGTAGCTGAAACTTACCGTTTTTTACCACACCAAGAAAAGAAAGAGGAGAAGGGTGCAGGCCTATCTGATTTAACATCCTGGAAGCATGCCAATGGTAGTGATTCTGGTGCTAATATGCCAACAGATGAAGTTTCTCAAGACTGGATTTCAGGTTTCGACCATTTCCAAATAAGCTTGGCGGGGTTTTGTGGTAAGTTGAGCAACTTTGCAGAGTCGTCTATGGAGGCGAATGTAGACCTGCAGAAGGCCATCCAGAGTGCTAAGAGCCATTATGAACAGTTTAAAGCTCAGTCTTCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

417

Amino Acids

47.29

Weight (kDa)

8.45

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 1 - 319 5.1e-98 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 922, 1194
AccI GTMKAC 1 cut(s) 1182
AciI CCGC 2 cut(s) 571, 1127
AclWI GGATC 4 cut(s) 64, 174, 459, 682
AcsI RAATTY 2 cut(s) 71, 268
AcuI CTGAAG 1 cut(s) 590
AfaI GTAC 3 cut(s) 149, 660, 836
AfiI CCNNNNNNNGG 2 cut(s) 297, 1192
AgsI TTSAA 4 cut(s) 8, 79, 373, 548
AjiI CACGTC 1 cut(s) 674
AjnI CCWGG 1 cut(s) 1025
Alw21I GWGCWC 2 cut(s) 534, 748
Alw26I GTCTC 1 cut(s) 516
AlwI GGATC 4 cut(s) 64, 174, 459, 682
AoxI GGCC 5 cut(s) 339, 830, 859, 1006, 1194
ApeKI GCWGC 1 cut(s) 391
ApoI RAATTY 2 cut(s) 71, 268
AspS9I GGNCC 1 cut(s) 860
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 4 cut(s) 110, 236, 868, 896
BanII GRGCYC 1 cut(s) 534
BauI CACGAG 1 cut(s) 435
BbsI GAAGAC 3 cut(s) 108, 804, 1233
Bbv12I GWGCWC 2 cut(s) 534, 748
BbvI GCAGC 1 cut(s) 403
BccI CCATC 3 cut(s) 343, 771, 1205
BceAI ACGGC 1 cut(s) 817
BciT130I CCWGG 1 cut(s) 1027
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 516
BfaI CTAG 2 cut(s) 395, 462
BfmI CTRYAG 3 cut(s) 768, 847, 1187
BfuAI ACCTGC 2 cut(s) 922, 1194
BisI GCNGC 1 cut(s) 392
BlsI GCNGC 1 cut(s) 393
BmcAI AGTACT 1 cut(s) 149
Bme1390I CCNGG 2 cut(s) 41, 1027
BmgBI CACGTC 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 860
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 2 cut(s) 41, 1027
BmsI GCATC 1 cut(s) 801
BoxI GACNNNNGTC 1 cut(s) 108
BpiI GAAGAC 3 cut(s) 108, 804, 1233
BpmI CTGGAG 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 1068
BpuMI CCSGG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 686
BsaJI CCNNGG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 1192
Bse1I ACTGG 4 cut(s) 244, 342, 893, 1094
Bse8I GATNNNNATC 1 cut(s) 686
BseBI CCWGG 1 cut(s) 1027
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 5 cut(s) 235, 579, 782, 1022, 1197
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 2 cut(s) 297, 1192
BseMII CTCAG 2 cut(s) 721, 1250
BseNI ACTGG 4 cut(s) 244, 342, 893, 1094
BseRI GAGGAG 2 cut(s) 120, 1007
BseXI GCAGC 1 cut(s) 403
BsgI GTGCAG 1 cut(s) 1023
BshFI GGCC 5 cut(s) 341, 832, 861, 1008, 1196
BsiHKAI GWGCWC 2 cut(s) 534, 748
BsiSI CCGG 1 cut(s) 41
BsiWI CGTACG 1 cut(s) 834
BslI CCNNNNNNNGG 2 cut(s) 297, 1192
BsmAI GTCTC 1 cut(s) 516
BsnI GGCC 5 cut(s) 341, 832, 861, 1008, 1196
Bsp1286I GDGCHC 2 cut(s) 534, 748
Bsp143I GATC 5 cut(s) 56, 179, 232, 451, 687
BspACI CCGC 2 cut(s) 571, 1127
BspANI GGCC 5 cut(s) 341, 832, 861, 1008, 1196
BspCNI CTCAG 2 cut(s) 720, 1249
BspLI GGNNCC 1 cut(s) 129
BspMAI CTGCAG 1 cut(s) 1191
BspMI ACCTGC 2 cut(s) 922, 1194
BspPI GGATC 4 cut(s) 64, 174, 459, 682
BsrI ACTGG 4 cut(s) 244, 342, 893, 1094
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 5 cut(s) 56, 179, 232, 451, 687
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 1 cut(s) 291
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 1027
Bst4CI ACNGT 4 cut(s) 663, 965, 1227, 1248
Bst6I CTCTTC 4 cut(s) 117, 190, 497, 543
BstC8I GCNNGC 2 cut(s) 1006, 1035
BstDEI CTNAG 3 cut(s) 707, 1209, 1236
BstEII GGTNACC 1 cut(s) 242
BstENI CCTNNNNNAGG 2 cut(s) 295, 1190
BstF5I GGATG 5 cut(s) 235, 579, 782, 1022, 1197
BstKTI GATC 5 cut(s) 59, 182, 235, 454, 690
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 5 cut(s) 56, 179, 232, 451, 687
BstNI CCWGG 1 cut(s) 1027
BstNSI RCATGY 1 cut(s) 1037
BstPAI GACNNNNGTC 1 cut(s) 108
BstPI GGTNACC 1 cut(s) 242
BstSCI CCNGG 2 cut(s) 39, 1025
BstSFI CTRYAG 3 cut(s) 768, 847, 1187
BstV1I GCAGC 1 cut(s) 403
BstV2I GAAGAC 3 cut(s) 108, 804, 1233
BstX2I RGATCY 1 cut(s) 451
BstXI CCANNNNNNTGG 2 cut(s) 349, 925
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 5 cut(s) 341, 832, 861, 1008, 1196
BtrI CACGTC 1 cut(s) 674
BtsCI GGATG 5 cut(s) 235, 579, 782, 1022, 1197
BtsIMutI CAGTG 2 cut(s) 349, 1244
BveI ACCTGC 2 cut(s) 922, 1194
Cac8I GCNNGC 2 cut(s) 1006, 1035
Cfr13I GGNCC 1 cut(s) 860
Csp6I GTAC 3 cut(s) 148, 659, 835
CviAII CATG 3 cut(s) 265, 281, 1034
CviQI GTAC 3 cut(s) 148, 659, 835
DdeI CTNAG 3 cut(s) 707, 1209, 1236
DpnI GATC 5 cut(s) 58, 181, 234, 453, 689
DpnII GATC 5 cut(s) 56, 179, 232, 451, 687
DraI TTTAAA 2 cut(s) 786, 1231
Eam1104I CTCTTC 4 cut(s) 117, 190, 497, 543
EarI CTCTTC 4 cut(s) 117, 190, 497, 543
EciI GGCGGA 1 cut(s) 586
Ecl136II GAGCTC 1 cut(s) 532
Eco130I CCWWGG 1 cut(s) 291
Eco147I AGGCCT 1 cut(s) 1008
Eco24I GRGCYC 1 cut(s) 534
Eco53kI GAGCTC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 590
Eco91I GGTNACC 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 532
EcoNI CCTNNNNNAGG 2 cut(s) 295, 1190
EcoO65I GGTNACC 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 1025
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 794
EcoT38I GRGCYC 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 3 cut(s) 268, 284, 1037
FalI AAGNNNNNCTT 4 cut(s) 184, 216, 743, 775
FatI CATG 3 cut(s) 264, 280, 1033
FauI CCCGC 1 cut(s) 1120
FblI GTMKAC 1 cut(s) 1182
Fnu4HI GCNGC 1 cut(s) 392
FokI GGATG 5 cut(s) 242, 586, 789, 1009, 1184
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 1 cut(s) 392
FspBI CTAG 2 cut(s) 395, 462
GluI GCNGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 5 cut(s) 341, 832, 861, 1008, 1196
HapII CCGG 1 cut(s) 41
Hin1II CATG 3 cut(s) 268, 284, 1037
HindIII AAGCTT 4 cut(s) 320, 602, 899, 1120
HinfI GANTC 8 cut(s) 196, 398, 465, 491, 668, 932, 1049, 1160
HpaII CCGG 1 cut(s) 41
HphI GGTGA 4 cut(s) 110, 236, 868, 896
Hpy166II GTNNAC 2 cut(s) 911, 1183
Hpy188I TCNGA 3 cut(s) 667, 681, 1015
Hpy188III TCNNGA 6 cut(s) 76, 119, 177, 462, 1085, 1201
Hpy8I GTNNAC 2 cut(s) 911, 1183
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 5 cut(s) 73, 351, 554, 991, 1186
HpyCH4III ACNGT 4 cut(s) 663, 965, 1227, 1248
HpyCH4IV ACGT 1 cut(s) 673
HpyCH4V TGCA 7 cut(s) 22, 264, 763, 792, 1004, 1157, 1189
HpyF3I CTNAG 3 cut(s) 707, 1209, 1236
HpySE526I ACGT 1 cut(s) 673
Hsp92II CATG 3 cut(s) 268, 284, 1037
Kzo9I GATC 5 cut(s) 56, 179, 232, 451, 687
LmnI GCTCC 4 cut(s) 133, 206, 537, 716
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 1 cut(s) 801
MaeI CTAG 2 cut(s) 395, 462
MaeII ACGT 1 cut(s) 673
MaeIII GTNAC 4 cut(s) 242, 596, 874, 884
MalI GATC 5 cut(s) 58, 181, 234, 453, 689
MboI GATC 5 cut(s) 56, 179, 232, 451, 687
MboII GAAGA 8 cut(s) 113, 134, 177, 514, 560, 804, 941, 1233
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 2 cut(s) 534, 748
MluCI AATT 5 cut(s) 33, 60, 71, 268, 486
MlyI GAGTC 2 cut(s) 485, 1169
MmeI TCCRAC 1 cut(s) 873
Mph1103I ATGCAT 1 cut(s) 794
MseI TTAA 9 cut(s) 152, 215, 386, 554, 566, 696, 785, 1019, 1230
MslI CAYNNNNRTG 1 cut(s) 1038
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 2 cut(s) 41, 1027
MvaI CCWGG 1 cut(s) 1027
NciI CCSGG 1 cut(s) 41
NdeII GATC 5 cut(s) 56, 179, 232, 451, 687
NlaIII CATG 3 cut(s) 268, 284, 1037
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 4 cut(s) 242, 596, 874, 884
NsiI ATGCAT 1 cut(s) 794
NspI RCATGY 1 cut(s) 1037
PaeI GCATGC 1 cut(s) 1037
PceI AGGCCT 1 cut(s) 1008
PfeI GAWTC 6 cut(s) 196, 398, 465, 668, 932, 1049
Pfl23II CGTACG 1 cut(s) 834
PfoI TCCNGGA 2 cut(s) 39, 1025
PkrI GCNGC 1 cut(s) 393
PleI GAGTC 2 cut(s) 485, 1168
PpsI GAGTC 2 cut(s) 485, 1168
PshAI GACNNNNGTC 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 1025
PspEI GGTNACC 1 cut(s) 242
PspGI CCWGG 1 cut(s) 1025
PspLI CGTACG 1 cut(s) 834
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 1 cut(s) 860
PstI CTGCAG 1 cut(s) 1191
PsuI RGATCY 1 cut(s) 451
RsaI GTAC 3 cut(s) 149, 660, 836
RsaNI GTAC 3 cut(s) 148, 659, 835
RseI CAYNNNNRTG 1 cut(s) 1038
SacI GAGCTC 1 cut(s) 534
SaqAI TTAA 9 cut(s) 152, 215, 386, 554, 566, 696, 785, 1019, 1230
SatI GCNGC 1 cut(s) 392
Sau3AI GATC 5 cut(s) 56, 179, 232, 451, 687
Sau96I GGNCC 1 cut(s) 860
ScaI AGTACT 1 cut(s) 149
SchI GAGTC 2 cut(s) 485, 1169
ScrFI CCNGG 2 cut(s) 41, 1027
SduI GDGCHC 2 cut(s) 534, 748
SfaNI GCATC 1 cut(s) 801
SfcI CTRYAG 3 cut(s) 768, 847, 1187
SmiMI CAYNNNNRTG 1 cut(s) 1038
SmlI CTYRAG 1 cut(s) 1083
SmoI CTYRAG 1 cut(s) 1083
SphI GCATGC 1 cut(s) 1037
Sse9I AATT 5 cut(s) 33, 60, 71, 268, 486
SseBI AGGCCT 1 cut(s) 1008
SsiI CCGC 2 cut(s) 571, 1127
SspMI CTAG 2 cut(s) 395, 462
SstI GAGCTC 1 cut(s) 534
StuI AGGCCT 1 cut(s) 1008
StyD4I CCNGG 2 cut(s) 39, 1025
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 4 cut(s) 663, 965, 1227, 1248
TaiI ACGT 1 cut(s) 676
TaqI TCGA 3 cut(s) 178, 500, 1104
TasI AATT 5 cut(s) 33, 60, 71, 268, 486
TatI WGTACW 1 cut(s) 147
TfiI GAWTC 6 cut(s) 196, 398, 465, 668, 932, 1049
Tru1I TTAA 9 cut(s) 152, 215, 386, 554, 566, 696, 785, 1019, 1230
Tru9I TTAA 9 cut(s) 152, 215, 386, 554, 566, 696, 785, 1019, 1230
TscAI CASTG 2 cut(s) 349, 1251
TseFI GTSAC 4 cut(s) 242, 596, 874, 884
TseI GCWGC 1 cut(s) 391
Tsp45I GTSAC 4 cut(s) 242, 596, 874, 884
TspDTI ATGAA 6 cut(s) 54, 281, 345, 746, 1089, 1236
TspGWI ACGGA 3 cut(s) 17, 646, 789
TspRI CASTG 2 cut(s) 349, 1251
XagI CCTNNNNNAGG 2 cut(s) 295, 1190
XapI RAATTY 2 cut(s) 71, 268
XbaI TCTAGA 1 cut(s) 461
XceI RCATGY 1 cut(s) 1037
XmiI GTMKAC 1 cut(s) 1182
XspI CTAG 2 cut(s) 395, 462
ZrmI AGTACT 1 cut(s) 149
Zsp2I ATGCAT 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.