MD11G1182900.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003248
Physical Location & Seq
Reverse (-)
23057010 .. 23058993
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1182900.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGGAGCCTCAAACTCTAAAATAGAAGAAGATAAGGCCCTGCAGCTATGTCGAGAAAGGAAGAAGTTTGTTAAACTGGCACTTGACGGCAGATGCTCGCTAGCAGCAGCTCATTTTATGTATATTCAATCACTAAAGAATACTGGAACAGCTCTTAGGAAATTTGTCGATCTTGGGGCTCCCGTTGAGTCTTCCTTGTACACTTCCACTAGTGCAACGCCAGAGCTTCTTGTTCAAACTGGCAAGAGTCTTTCTCAATTCTCTTTTTCTTCTCCATCTGTGTCACAACGCATAGATGCAGCTGAAACCTTTTTTCCATCTCCCTCTCCTCCAAATTCTACACGATTTCAGGCAAATCATATGAAGGGCAGCTTTTCTAAAACAGTTGAAGAAAAACCTCCTATTCCCCTTACTGCAACAGTAACCTCCTCGAGCACCCCACAGAATACCACACCTCGATCTGCTGGAAAACCTGAAACGTCACCAATTGAAGCTTCTTCACTCCCACCTCAAACTCCACCGTGGGATTACTTTGATTTTTTCCATCCAATTGACCATCAATTTTCTTTTCAAGAAGGGAAGGGAATGAACCAGGGGTTTGAAAATAACGATGATAGACAGCTTAGGGAAGAGGAGGGAATTCCTGATATGGAAGATGAAGAAGAGAAGGCTTCTTTTCATGGAAGGGAAGAATCTCAGGATTCTGAAGATGAATTCGATGAGCCTGCTACAAATACCCTTGTTCGAAGTTTTGAAAATCTTAATAGAGTGCAGGATCACATTGAAGCTAGTGCTTCACCTACAATGCCTTCTGCAGGAAGTGTAACTTCAGAAACTGAGTTATTGAATGGGGAGAAAAGTAACTCACCTGATTTTTCACCATTGAGAGCCACATCCTCACCAGTTGCAGTTGAAACTGACTCAACTAAAAAAACAGTTAAGAAGATTGCATTGAAAGTAAGGCTGCCCCAAAAGATTTTTTTTCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

331

Amino Acids

36.35

Weight (kDa)

5.11

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 57 2.6e-24 Protein of unknown function (DUF630)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 783
AcsI RAATTY 4 cut(s) 161, 334, 639, 713
AcuI CTGAAG 2 cut(s) 726, 813
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 815
AhlI ACTAGT 1 cut(s) 209
AjnI CCWGG 1 cut(s) 591
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AluBI AGCT 9 cut(s) 46, 110, 152, 226, 302, 372, 494, 622, 788
AluI AGCT 9 cut(s) 46, 110, 152, 226, 302, 372, 494, 622, 788
Alw21I GWGCWC 1 cut(s) 437
AlwI GGATC 1 cut(s) 783
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 6 cut(s) 43, 104, 107, 299, 369, 964
ApoI RAATTY 4 cut(s) 161, 334, 639, 713
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 5 cut(s) 474, 789, 858, 870, 891
AsuII TTCGAA 1 cut(s) 745
AsuNHI GCTAGC 1 cut(s) 100
AvaI CYCGRG 1 cut(s) 430
BanII GRGCYC 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 6 cut(s) 55, 116, 119, 311, 381, 951
BccI CCATC 4 cut(s) 283, 325, 552, 564
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 4 cut(s) 32, 66, 707, 741
BciT130I CCWGG 1 cut(s) 593
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 3 cut(s) 101, 210, 789
BfmI CTRYAG 2 cut(s) 41, 813
BisI GCNGC 6 cut(s) 44, 105, 108, 300, 370, 965
BlsI GCNGC 6 cut(s) 45, 106, 109, 301, 371, 966
Bme1390I CCNGG 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 7, 180
BmrFI CCNGG 1 cut(s) 593
BmsI GCATC 2 cut(s) 83, 286
BmtI GCTAGC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 238, 270
Bpu10I CCTNAGC 1 cut(s) 623
Bpu14I TTCGAA 1 cut(s) 745
BsaJI CCNNGG 2 cut(s) 521, 592
Bsc4I CCNNNNNNNGG 1 cut(s) 815
Bse1I ACTGG 4 cut(s) 81, 148, 244, 902
BseBI CCWGG 1 cut(s) 593
BseDI CCNNGG 2 cut(s) 521, 592
BseGI GGATG 2 cut(s) 544, 893
BseLI CCNNNNNNNGG 1 cut(s) 815
BseMII CTCAG 2 cut(s) 710, 828
BseNI ACTGG 4 cut(s) 81, 148, 244, 902
BseRI GAGGAG 3 cut(s) 318, 418, 647
BseXI GCAGC 6 cut(s) 55, 116, 119, 311, 381, 951
BsgI GTGCAG 1 cut(s) 791
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 430
BslI CCNNNNNNNGG 1 cut(s) 815
BsnI GGCC 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 745
Bsp1286I GDGCHC 2 cut(s) 181, 437
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 3 cut(s) 169, 458, 775
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 2 cut(s) 709, 829
BspLI GGNNCC 2 cut(s) 7, 180
BspMAI CTGCAG 2 cut(s) 45, 817
BspOI GCTAGC 1 cut(s) 104
BspPI GGATC 1 cut(s) 783
BspT104I TTCGAA 1 cut(s) 745
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 4 cut(s) 81, 148, 244, 902
BssECI CCNNGG 2 cut(s) 521, 592
BssMI GATC 3 cut(s) 169, 458, 775
Bst2UI CCWGG 1 cut(s) 593
Bst4CI ACNGT 4 cut(s) 385, 421, 522, 937
Bst6I CTCTTC 2 cut(s) 624, 657
BstAUI TGTACA 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 745
BstC8I GCNNGC 3 cut(s) 98, 102, 726
BstDEI CTNAG 4 cut(s) 155, 623, 696, 837
BstDSI CCRYGG 1 cut(s) 521
BstENI CCTNNNNNAGG 1 cut(s) 813
BstF5I GGATG 2 cut(s) 544, 893
BstKTI GATC 3 cut(s) 172, 461, 778
BstMBI GATC 3 cut(s) 169, 458, 775
BstNI CCWGG 1 cut(s) 593
BstSCI CCNGG 1 cut(s) 591
BstSFI CTRYAG 2 cut(s) 41, 813
BstV1I GCAGC 6 cut(s) 55, 116, 119, 311, 381, 951
BstV2I GAAGAC 1 cut(s) 183
BsuRI GGCC 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 521
BtsCI GGATG 2 cut(s) 544, 893
Cac8I GCNNGC 3 cut(s) 98, 102, 726
CaiI CAGNNNCTG 1 cut(s) 836
Cfr13I GGNCC 1 cut(s) 37
Csp6I GTAC 1 cut(s) 199
CviAII CATG 2 cut(s) 680, 990
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 4 cut(s) 155, 623, 696, 837
DpnI GATC 3 cut(s) 171, 460, 777
DpnII GATC 3 cut(s) 169, 458, 775
Eam1104I CTCTTC 2 cut(s) 624, 657
EarI CTCTTC 2 cut(s) 624, 657
Eco24I GRGCYC 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 726, 813
Eco88I CYCGRG 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 813
EcoO109I RGGNCCY 1 cut(s) 37
EcoRI GAATTC 2 cut(s) 639, 713
EcoRII CCWGG 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 181
FaeI CATG 2 cut(s) 683, 993
FaiI YATR 9 cut(s) 49, 119, 123, 293, 360, 362, 650, 681, 991
FalI AAGNNNNNCTT 4 cut(s) 356, 388, 811, 843
FatI CATG 2 cut(s) 679, 989
FauNDI CATATG 1 cut(s) 360
Fnu4HI GCNGC 6 cut(s) 44, 105, 108, 300, 370, 965
FokI GGATG 2 cut(s) 531, 880
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 6 cut(s) 44, 105, 108, 300, 370, 965
FspBI CTAG 3 cut(s) 101, 210, 789
GluI GCNGC 6 cut(s) 44, 105, 108, 300, 370, 965
HaeIII GGCC 1 cut(s) 38
Hin1II CATG 2 cut(s) 683, 993
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 5 cut(s) 188, 247, 692, 701, 920
HphI GGTGA 5 cut(s) 474, 789, 858, 870, 891
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 2 cut(s) 706, 832
Hpy188III TCNNGA 4 cut(s) 53, 572, 644, 698
Hpy8I GTNNAC 1 cut(s) 201
HpyAV CCTTC 6 cut(s) 358, 569, 574, 661, 678, 819
HpyCH4III ACNGT 4 cut(s) 385, 421, 522, 937
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 8 cut(s) 43, 215, 299, 416, 772, 815, 908, 950
HpyF3I CTNAG 4 cut(s) 155, 623, 696, 837
HpySE526I ACGT 1 cut(s) 479
Hsp92II CATG 2 cut(s) 683, 993
Kzo9I GATC 3 cut(s) 169, 458, 775
LmnI GCTCC 2 cut(s) 5, 184
Lsp1109I GCAGC 6 cut(s) 55, 116, 119, 311, 381, 951
LweI GCATC 2 cut(s) 83, 286
MaeI CTAG 3 cut(s) 101, 210, 789
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 5 cut(s) 282, 421, 480, 823, 860
MalI GATC 3 cut(s) 171, 460, 777
MboI GATC 3 cut(s) 169, 458, 775
MfeI CAATTG 2 cut(s) 486, 549
MhlI GDGCHC 2 cut(s) 181, 437
MluCI AATT 8 cut(s) 161, 257, 334, 486, 549, 560, 639, 713
MlyI GAGTC 3 cut(s) 197, 256, 914
MseI TTAA 3 cut(s) 72, 762, 939
MspA1I CMGCKG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 593
MunI CAATTG 2 cut(s) 486, 549
MvaI CCWGG 1 cut(s) 593
NdeI CATATG 1 cut(s) 360
NdeII GATC 3 cut(s) 169, 458, 775
NheI GCTAGC 1 cut(s) 100
NlaIII CATG 2 cut(s) 683, 993
NlaIV GGNNCC 2 cut(s) 7, 180
NmuCI GTSAC 2 cut(s) 282, 480
NspV TTCGAA 1 cut(s) 745
PaeR7I CTCGAG 1 cut(s) 430
PfeI GAWTC 2 cut(s) 692, 701
PkrI GCNGC 6 cut(s) 45, 106, 109, 301, 371, 966
PleI GAGTC 3 cut(s) 196, 255, 914
PpsI GAGTC 3 cut(s) 196, 255, 914
Psp6I CCWGG 1 cut(s) 591
PspGI CCWGG 1 cut(s) 591
PspN4I GGNNCC 2 cut(s) 7, 180
PspPI GGNCC 1 cut(s) 37
PspXI VCTCGAGB 1 cut(s) 430
PstI CTGCAG 2 cut(s) 45, 817
PstNI CAGNNNCTG 1 cut(s) 836
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SaqAI TTAA 3 cut(s) 72, 762, 939
SatI GCNGC 6 cut(s) 44, 105, 108, 300, 370, 965
Sau3AI GATC 3 cut(s) 169, 458, 775
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 3 cut(s) 197, 256, 914
ScrFI CCNGG 1 cut(s) 593
SduI GDGCHC 2 cut(s) 181, 437
SfaNI GCATC 2 cut(s) 83, 286
SfcI CTRYAG 2 cut(s) 41, 813
Sfr274I CTCGAG 1 cut(s) 430
SfuI TTCGAA 1 cut(s) 745
SlaI CTCGAG 1 cut(s) 430
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
SpeI ACTAGT 1 cut(s) 209
Sse9I AATT 8 cut(s) 161, 257, 334, 486, 549, 560, 639, 713
SspMI CTAG 3 cut(s) 101, 210, 789
StyD4I CCNGG 1 cut(s) 591
TaaI ACNGT 4 cut(s) 385, 421, 522, 937
TaiI ACGT 1 cut(s) 482
TaqI TCGA 6 cut(s) 52, 168, 431, 457, 717, 745
TasI AATT 8 cut(s) 161, 257, 334, 486, 549, 560, 639, 713
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 2 cut(s) 692, 701
Tru1I TTAA 3 cut(s) 72, 762, 939
Tru9I TTAA 3 cut(s) 72, 762, 939
TseFI GTSAC 2 cut(s) 282, 480
TseI GCWGC 6 cut(s) 43, 104, 107, 299, 369, 964
Tsp45I GTSAC 2 cut(s) 282, 480
TspDTI ATGAA 5 cut(s) 377, 602, 668, 672, 726
XagI CCTNNNNNAGG 1 cut(s) 813
XapI RAATTY 4 cut(s) 161, 334, 639, 713
XhoI CTCGAG 1 cut(s) 430
XspI CTAG 3 cut(s) 101, 210, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.