Rorug05G0256300

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003248
Physical Location & Seq
Forward (+)
28766240 .. 28770429
4190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0256300.1

Sequence Viewer

Length: 1344 bp
ATGGACCTTCTCTGCAACGCATACTCAAACGCCTCGGACAACGACGACGACGAAGAAGAAGAAGAAGAAGCTCGACCACTTAAACAACCGAGACCCGAAACCTCCAAACCCCCGAATTGGAAGCAACACAAACCCAATAACCCATTTCAGTATACCAACACTTACCATCCTCCGCCGGCCGCAACCCAGTTTCCGGTCATCCGTACTGAGACCCAAGTCCCTGGTAGATACATATCCAAGAGAGAGCGGGCTCTATTGGGCTCCCAGCCCAGCATCCCCGACCCGAACCCAAACCCGAATACCCTGGCCACCACCTCCCCTGTGTTGGGGAGTATTTCGGATTCTGATGTTCGTCGCGATATTGTGTCATTGTTGAGAAATAAAGCAAAGGTCTGTTCAAAGCCTGGAAAAATGCCTGAAAGAATGTCTGTGGGTCTAAATAGACATACCAAAGCTGTCAATGCGGTACATTGGTCACCTAGTCATGCTCATCTTCTAGCCTCTGCTTCGATGGATCACACAATCTGCATATGGAGTGTGTGGAACAGTGACCAGAAGGTAGCCCGTGTGTTGAATTTTCACAATGCAGCAGTTAAAGATGTAAAATGGTCGCGGGATGGATTGTTTGTGCTTTCTTGCGGATACGACTCCTCTTCAAGGTTAGTGGATGTTGAAAAGGGGATAGAGACTCAAGTTTTTAAGGAGGATCAAGTTGTCAGTGTTGTTAAGTTCCATCCTGACAGTTCAAAACTCTTCATTGCTGGGGGATCAAGAGGCAGCCTCAAGCTGTGGGATGTTAGAAATGGCAAGGTGGTCCATGACTACATTCGAGGTCATGATCCTATCCTTGATGTCGAATTCACCAAGAATGGCAAGCAGATTATTTCCTCGAGTGATGTATCTGGACGAAACCTTAGTGAGAATTCTATTGTTGTTTGGGATGTTTCACGAGAAGTTCCTCTCTCTAATCAGGTGTACGTAGAAGCATTCACCTGTCCCTGTGTTAGATTCCACCCGTTCGAACAATCGTTTGTTGCACAGTCAAATGGTAATTATATTGCAATCTTCTCTGCAAGGCCTCCCTTCAAGATGAACAAGTACAAGAGGTACGAAAGCCATGGGGTATCAGGGTTTCCAATAAAATGTGGATTTAGCTTGGATGGGGAAAAGCTATTGTCAGGCTCCTCAGATGGTTCAATATACTTCTATGATTATGGGTCTTCTGAGGTTATCAAGAAAATCAAGGCATTTGAGCAGGCCTGCATAGATATAGCGGTCCACCCGATCATACCTAATGTGATTGCTGCGTGCAATTGGAACGGAGAAGTTTCTGTATTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

447

Amino Acids

49.88

Weight (kDa)

7.21

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 137 - 274 5.7e-13 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 141 - 232 2.1e-10 WDR3 first beta-propeller domain
Beta-prop_WDR35_TULP_N PF24797 142 - 273 5.6e-06 WDR35/TULP4 N-terminal
WD40_Prp19 PF24814 142 - 357 1.8e-23 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 143 - 183 1.6e-07 THOC3 beta-propeller domain
Beta-prop_EML PF23409 144 - 296 7.3e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 146 - 179 5e-07 WD domain, G-beta repeat
EIF3I PF24805 146 - 252 8.7e-07 EIF3I
WD40_MABP1-WDR62_2nd PF24782 146 - 299 2.1e-08 MABP1/WDR62 second WD40 domain
Beta-prop_WDR5 PF25175 146 - 445 3.3e-42 WDR5 beta-propeller domain
WDR55 PF24796 151 - 299 3.2e-18 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 162 - 251 1.8e-06 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 184 - 301 4.8e-09 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 186 - 317 5.3e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 194 - 423 4.8e-23 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 223 - 321 1.5e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 278 - 444 1.3e-08 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 247
AccI GTMKAC 1 cut(s) 152
AccII CGCG 2 cut(s) 357, 613
AciI CCGC 7 cut(s) 173, 180, 247, 464, 613, 639, 1274
AclWI GGATC 4 cut(s) 522, 714, 775, 833
AcoI YGGCCR 2 cut(s) 177, 306
AcsI RAATTY 3 cut(s) 574, 857, 922
AfaI GTAC 5 cut(s) 205, 468, 977, 1100, 1109
AfiI CCNNNNNNNGG 5 cut(s) 117, 193, 325, 326, 657
AgsI TTSAA 7 cut(s) 399, 574, 657, 674, 747, 1087, 1197
AjnI CCWGG 3 cut(s) 220, 303, 403
AluBI AGCT 5 cut(s) 71, 455, 787, 1155, 1171
AluI AGCT 5 cut(s) 71, 455, 787, 1155, 1171
Alw26I GTCTC 3 cut(s) 85, 203, 680
AlwI GGATC 4 cut(s) 522, 714, 775, 833
Ama87I CYCGRG 1 cut(s) 889
AoxI GGCC 4 cut(s) 177, 306, 1076, 1257
ApeKI GCWGC 3 cut(s) 587, 777, 1304
ApoI RAATTY 3 cut(s) 574, 857, 922
AspS9I GGNCC 3 cut(s) 4, 814, 1276
AsuHPI GGTGA 3 cut(s) 468, 853, 982
AsuII TTCGAA 1 cut(s) 1020
AvaI CYCGRG 1 cut(s) 889
AvaII GGWCC 3 cut(s) 4, 814, 1276
BaeI ACNNNNGTAYC 2 cut(s) 458, 491
BalI TGGCCA 1 cut(s) 308
BanII GRGCYC 2 cut(s) 253, 263
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 1 cut(s) 1212
BbvI GCAGC 3 cut(s) 599, 789, 1291
BccI CCATC 6 cut(s) 174, 505, 611, 741, 1154, 1184
BciT130I CCWGG 3 cut(s) 222, 305, 405
BciVI GTATCC 1 cut(s) 635
BcoDI GTCTC 3 cut(s) 85, 203, 680
BfaI CTAG 2 cut(s) 480, 497
BfuI GTATCC 1 cut(s) 635
BisI GCNGC 4 cut(s) 180, 588, 778, 1305
BlsI GCNGC 4 cut(s) 181, 589, 779, 1306
Bme1390I CCNGG 3 cut(s) 222, 305, 405
Bme18I GGWCC 3 cut(s) 4, 814, 1276
BmeT110I CYCGRG 1 cut(s) 889
BmgT120I GGNCC 3 cut(s) 4, 814, 1276
BmiI GGNNCC 2 cut(s) 262, 1183
BmrFI CCNGG 3 cut(s) 222, 305, 405
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 282
BmuI ACTGGG 1 cut(s) 181
BoxI GACNNNNGTC 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 1212
BplI GAGNNNNNCTC 2 cut(s) 765, 797
Bpu14I TTCGAA 1 cut(s) 1020
BpuEI CTTGAG 2 cut(s) 675, 767
BsaAI YACGTR 1 cut(s) 979
BsaBI GATNNNNATC 1 cut(s) 232
BsaI GGTCTC 2 cut(s) 85, 203
BsaJI CCNNGG 4 cut(s) 33, 220, 303, 1117
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 193, 325, 326, 657
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 187
Bse3DI GCAATG 1 cut(s) 756
Bse8I GATNNNNATC 1 cut(s) 232
BseBI CCWGG 3 cut(s) 222, 305, 405
BseDI CCNNGG 4 cut(s) 33, 220, 303, 1117
BseGI GGATG 9 cut(s) 166, 198, 273, 622, 673, 733, 799, 946, 1165
BseJI GATNNNNATC 1 cut(s) 232
BseLI CCNNNNNNNGG 5 cut(s) 117, 193, 325, 326, 657
BseMI GCAATG 1 cut(s) 756
BseMII CTCAG 3 cut(s) 198, 1200, 1215
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 2 cut(s) 640, 1174
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 3 cut(s) 599, 789, 1291
BseYI CCCAGC 3 cut(s) 264, 269, 761
Bsh1236I CGCG 2 cut(s) 357, 613
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 4 cut(s) 179, 308, 1078, 1259
BsiEI CGRYCG 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 889
BsiSI CCGG 2 cut(s) 176, 194
BslFI GGGAC 2 cut(s) 203, 981
BslI CCNNNNNNNGG 5 cut(s) 117, 193, 325, 326, 657
BsmAI GTCTC 3 cut(s) 85, 203, 680
BsmFI GGGAC 2 cut(s) 203, 981
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 4 cut(s) 179, 308, 1078, 1259
Bso31I GGTCTC 2 cut(s) 85, 203
BsoBI CYCGRG 1 cut(s) 889
Bsp119I TTCGAA 1 cut(s) 1020
Bsp1286I GDGCHC 2 cut(s) 253, 263
Bsp143I GATC 5 cut(s) 514, 706, 767, 838, 1284
Bsp19I CCATGG 1 cut(s) 1117
Bsp68I TCGCGA 1 cut(s) 357
BspACI CCGC 7 cut(s) 173, 180, 247, 464, 613, 639, 1274
BspANI GGCC 4 cut(s) 179, 308, 1078, 1259
BspCNI CTCAG 3 cut(s) 199, 1199, 1216
BspFNI CGCG 2 cut(s) 357, 613
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 2 cut(s) 262, 1183
BspPI GGATC 4 cut(s) 522, 714, 775, 833
BspT104I TTCGAA 1 cut(s) 1020
BspTNI GGTCTC 2 cut(s) 85, 203
BsrBI CCGCTC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 756
BsrFI RCCGGY 1 cut(s) 175
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 4 cut(s) 33, 220, 303, 1117
BssMI GATC 5 cut(s) 514, 706, 767, 838, 1284
BssNAI GTATAC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 948
BssT1I CCWWGG 1 cut(s) 1117
Bst1107I GTATAC 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 948
Bst2UI CCWGG 3 cut(s) 222, 305, 405
Bst4CI ACNGT 3 cut(s) 548, 743, 1041
Bst6I CTCTTC 2 cut(s) 658, 758
BstBAI YACGTR 1 cut(s) 979
BstBI TTCGAA 1 cut(s) 1020
BstC8I GCNNGC 6 cut(s) 177, 249, 875, 1257, 1261, 1309
BstDEI CTNAG 4 cut(s) 207, 914, 1186, 1224
BstDSI CCRYGG 1 cut(s) 1117
BstEII GGTNACC 1 cut(s) 474
BstENI CCTNNNNNAGG 1 cut(s) 655
BstF5I GGATG 9 cut(s) 166, 198, 273, 622, 673, 733, 799, 946, 1165
BstFNI CGCG 2 cut(s) 357, 613
BstKTI GATC 5 cut(s) 517, 709, 770, 841, 1287
BstMAI GTCTC 3 cut(s) 85, 203, 680
BstMBI GATC 5 cut(s) 514, 706, 767, 838, 1284
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNI CCWGG 3 cut(s) 222, 305, 405
BstPAI GACNNNNGTC 1 cut(s) 215
BstPI GGTNACC 1 cut(s) 474
BstSCI CCNGG 3 cut(s) 220, 303, 403
BstSNI TACGTA 1 cut(s) 979
BstUI CGCG 2 cut(s) 357, 613
BstV1I GCAGC 3 cut(s) 599, 789, 1291
BstV2I GAAGAC 1 cut(s) 1212
BstXI CCANNNNNNTGG 1 cut(s) 221
BstZ17I GTATAC 1 cut(s) 153
BstZI CGGCCG 1 cut(s) 177
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 4 cut(s) 179, 308, 1078, 1259
BtgI CCRYGG 1 cut(s) 1117
BtsCI GGATG 9 cut(s) 166, 198, 273, 622, 673, 733, 799, 946, 1165
BtsIMutI CAGTG 2 cut(s) 553, 724
BtuMI TCGCGA 1 cut(s) 357
Cac8I GCNNGC 6 cut(s) 177, 249, 875, 1257, 1261, 1309
CciI TCATGA 1 cut(s) 835
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 3 cut(s) 4, 814, 1276
Csp6I GTAC 5 cut(s) 204, 467, 976, 1099, 1108
CspCI CAANNNNNGTGG 2 cut(s) 645, 680
CviAII CATG 4 cut(s) 485, 818, 836, 1118
CviQI GTAC 5 cut(s) 204, 467, 976, 1099, 1108
DdeI CTNAG 4 cut(s) 207, 914, 1186, 1224
DpnI GATC 5 cut(s) 516, 708, 769, 840, 1286
DpnII GATC 5 cut(s) 514, 706, 767, 838, 1284
EaeI YGGCCR 2 cut(s) 177, 306
EagI CGGCCG 1 cut(s) 177
Eam1104I CTCTTC 2 cut(s) 658, 758
EarI CTCTTC 2 cut(s) 658, 758
EciI GGCGGA 1 cut(s) 162
EclXI CGGCCG 1 cut(s) 177
Eco105I TACGTA 1 cut(s) 979
Eco130I CCWWGG 1 cut(s) 1117
Eco147I AGGCCT 2 cut(s) 1078, 1259
Eco24I GRGCYC 2 cut(s) 253, 263
Eco31I GGTCTC 2 cut(s) 85, 203
Eco47I GGWCC 3 cut(s) 4, 814, 1276
Eco52I CGGCCG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 889
Eco91I GGTNACC 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 655
EcoO65I GGTNACC 1 cut(s) 474
EcoRI GAATTC 2 cut(s) 857, 922
EcoRII CCWGG 3 cut(s) 220, 303, 403
EcoT14I CCWWGG 1 cut(s) 1117
EcoT38I GRGCYC 2 cut(s) 253, 263
ErhI CCWWGG 1 cut(s) 1117
FaeI CATG 4 cut(s) 488, 821, 839, 1121
FaqI GGGAC 2 cut(s) 203, 981
FatI CATG 4 cut(s) 484, 817, 835, 1117
FauI CCCGC 2 cut(s) 240, 606
FauNDI CATATG 1 cut(s) 530
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 4 cut(s) 180, 588, 778, 1305
FokI GGATG 9 cut(s) 153, 185, 260, 629, 680, 720, 806, 953, 1172
FriOI GRGCYC 2 cut(s) 253, 263
Fsp4HI GCNGC 4 cut(s) 180, 588, 778, 1305
FspBI CTAG 2 cut(s) 480, 497
GluI GCNGC 4 cut(s) 180, 588, 778, 1305
GsaI CCCAGC 3 cut(s) 268, 273, 765
HaeIII GGCC 4 cut(s) 179, 308, 1078, 1259
HapII CCGG 2 cut(s) 176, 194
Hin1II CATG 4 cut(s) 488, 821, 839, 1121
HinfI GANTC 4 cut(s) 341, 647, 688, 1008
HpaII CCGG 2 cut(s) 176, 194
HphI GGTGA 3 cut(s) 468, 853, 982
Hpy166II GTNNAC 3 cut(s) 153, 976, 1279
Hpy188I TCNGA 5 cut(s) 37, 340, 346, 1189, 1225
Hpy188III TCNNGA 8 cut(s) 356, 737, 771, 836, 903, 948, 1087, 1234
Hpy8I GTNNAC 3 cut(s) 153, 976, 1279
Hpy99I CGWCG 4 cut(s) 47, 50, 53, 357
HpyAV CCTTC 3 cut(s) 17, 550, 1093
HpyCH4III ACNGT 3 cut(s) 548, 743, 1041
HpyCH4IV ACGT 1 cut(s) 978
HpyCH4V TGCA 8 cut(s) 15, 528, 587, 1037, 1061, 1073, 1263, 1311
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 4 cut(s) 207, 914, 1186, 1224
HpySE526I ACGT 1 cut(s) 978
Hsp92II CATG 4 cut(s) 488, 821, 839, 1121
KroI GCCGGC 1 cut(s) 175
KroNI GCCGGC 1 cut(s) 177
Kzo9I GATC 5 cut(s) 514, 706, 767, 838, 1284
LmnI GCTCC 2 cut(s) 266, 1187
Lsp1109I GCAGC 3 cut(s) 599, 789, 1291
LweI GCATC 1 cut(s) 282
MaeI CTAG 2 cut(s) 480, 497
MaeII ACGT 1 cut(s) 978
MaeIII GTNAC 2 cut(s) 474, 548
MalI GATC 5 cut(s) 516, 708, 769, 840, 1286
MbiI CCGCTC 1 cut(s) 247
MboI GATC 5 cut(s) 514, 706, 767, 838, 1284
MfeI CAATTG 1 cut(s) 1312
MhlI GDGCHC 2 cut(s) 253, 263
MlsI TGGCCA 1 cut(s) 308
MluCI AATT 6 cut(s) 115, 574, 857, 922, 1051, 1312
MluNI TGGCCA 1 cut(s) 308
MlyI GAGTC 2 cut(s) 641, 682
Mox20I TGGCCA 1 cut(s) 308
MroNI GCCGGC 1 cut(s) 175
MscI TGGCCA 1 cut(s) 308
MseI TTAA 4 cut(s) 81, 594, 699, 726
Msp20I TGGCCA 1 cut(s) 308
MspI CCGG 2 cut(s) 176, 194
MspR9I CCNGG 3 cut(s) 222, 305, 405
MunI CAATTG 1 cut(s) 1312
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 3 cut(s) 222, 305, 405
MvnI CGCG 2 cut(s) 357, 613
MwoI GCNNNNNNNGC 1 cut(s) 461
NaeI GCCGGC 1 cut(s) 177
NcoI CCATGG 1 cut(s) 1117
NdeI CATATG 1 cut(s) 530
NdeII GATC 5 cut(s) 514, 706, 767, 838, 1284
NgoMIV GCCGGC 1 cut(s) 175
NlaIII CATG 4 cut(s) 488, 821, 839, 1121
NlaIV GGNNCC 2 cut(s) 262, 1183
NmuCI GTSAC 2 cut(s) 474, 548
NruI TCGCGA 1 cut(s) 357
NspV TTCGAA 1 cut(s) 1020
PaeR7I CTCGAG 1 cut(s) 889
PagI TCATGA 1 cut(s) 835
PceI AGGCCT 2 cut(s) 1078, 1259
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 986
PdiI GCCGGC 1 cut(s) 177
PfeI GAWTC 2 cut(s) 341, 1008
PkrI GCNGC 4 cut(s) 181, 589, 779, 1306
PleI GAGTC 2 cut(s) 641, 682
PpsI GAGTC 2 cut(s) 641, 682
Ppu21I YACGTR 1 cut(s) 979
PshAI GACNNNNGTC 1 cut(s) 215
Psp6I CCWGG 3 cut(s) 220, 303, 403
PspEI GGTNACC 1 cut(s) 474
PspFI CCCAGC 3 cut(s) 264, 269, 761
PspGI CCWGG 3 cut(s) 220, 303, 403
PspN4I GGNNCC 2 cut(s) 262, 1183
PspPI GGNCC 3 cut(s) 4, 814, 1276
PspXI VCTCGAGB 1 cut(s) 889
RruI TCGCGA 1 cut(s) 357
RsaI GTAC 5 cut(s) 205, 468, 977, 1100, 1109
RsaNI GTAC 5 cut(s) 204, 467, 976, 1099, 1108
SaqAI TTAA 4 cut(s) 81, 594, 699, 726
SatI GCNGC 4 cut(s) 180, 588, 778, 1305
Sau3AI GATC 5 cut(s) 514, 706, 767, 838, 1284
Sau96I GGNCC 3 cut(s) 4, 814, 1276
SchI GAGTC 2 cut(s) 641, 682
ScrFI CCNGG 3 cut(s) 222, 305, 405
SduI GDGCHC 2 cut(s) 253, 263
SfaNI GCATC 1 cut(s) 282
Sfr274I CTCGAG 1 cut(s) 889
SfuI TTCGAA 1 cut(s) 1020
SinI GGWCC 3 cut(s) 4, 814, 1276
SlaI CTCGAG 1 cut(s) 889
SmlI CTYRAG 3 cut(s) 690, 782, 889
SmoI CTYRAG 3 cut(s) 690, 782, 889
SnaBI TACGTA 1 cut(s) 979
Sse9I AATT 6 cut(s) 115, 574, 857, 922, 1051, 1312
SseBI AGGCCT 2 cut(s) 1078, 1259
SsiI CCGC 7 cut(s) 173, 180, 247, 464, 613, 639, 1274
SspMI CTAG 2 cut(s) 480, 497
StuI AGGCCT 2 cut(s) 1078, 1259
StyD4I CCNGG 3 cut(s) 220, 303, 403
StyI CCWWGG 1 cut(s) 1117
TaaI ACNGT 3 cut(s) 548, 743, 1041
TaiI ACGT 1 cut(s) 981
TaqI TCGA 6 cut(s) 73, 509, 829, 855, 890, 1020
TasI AATT 6 cut(s) 115, 574, 857, 922, 1051, 1312
TatI WGTACW 1 cut(s) 1098
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 2 cut(s) 341, 1008
Tru1I TTAA 4 cut(s) 81, 594, 699, 726
Tru9I TTAA 4 cut(s) 81, 594, 699, 726
TscAI CASTG 2 cut(s) 553, 724
TseFI GTSAC 2 cut(s) 474, 548
TseI GCWGC 3 cut(s) 587, 777, 1304
Tsp45I GTSAC 2 cut(s) 474, 548
TspDTI ATGAA 2 cut(s) 745, 1106
TspGWI ACGGA 2 cut(s) 191, 1335
TspRI CASTG 2 cut(s) 553, 724
VpaK11BI GGWCC 3 cut(s) 4, 814, 1276
XagI CCTNNNNNAGG 1 cut(s) 655
XapI RAATTY 3 cut(s) 574, 857, 922
XhoI CTCGAG 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 152
XspI CTAG 2 cut(s) 480, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.