Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0014891

Physical Location & Seq

Genomic Coordinates Forward (+)

39513361 .. 39525489

Gene Structure 12129 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1278100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3468 bp

ATGGCAAGCAGCAGTGACTCGGCCTACCACTGCGGCAAATCGCTCCTCGACGAACTTGACACCGGCCTCCGAGCTAAGAGAAGGTGGCGGATGGCCTTTTTGGCATTACGTGTTGTCCACGTCATGCTTGAGCTTCCCAAGATAGTATCCAGGACAAAAAAATATGGAGACGATGAAACTGGTCGCTACAGTTTTGTGGTCGACTTTGATGATCATGAAGATGCTGGACTTGCAGAAATTGTGAGGGAAAAGAACTCGGCTGCTACACAAAGCCACGATTCGCCTTATGGTTATGAAAAAATGCTCCTCGACATCACCACCACCACCCTCACCAGAGCCAAGATGAGATGGCGCATGGCCTACGCAGCAATATATGCTGTTCGGGCAATGCTTTCGCTTCCCAGAGAGCTTCTAAACAAGAGAAACAACTACAAAAAGGACCCCGAGATCTTGCACTCTTATTCTTACCCTGCACTGGACGTGAAACCAAGCACTGCCCACCAAGATATCAATACTGAAACTGATCGCCGTAGTTCCCTCCCCAACATTGAATTTGATCATGCAGGACTCGTACAAATGGTGAAGGATAAAGACTTGGCTGCTCTAAATGAGTCTGGAGGAGTTATCGGCGTGGCCACTTCTCTTGGGACAAACCCTAAAAATGGAATTCATGGAAATGAGTTAGAAGTCAACAGAAGGCGTGAAATCTTTGGTTCAAACACCTACCACAGGCAGCCTCCAAAAGGGTTGCTCTACTTTGTCCTCGACGCTTTAAAAGACACAACAATTCTGATCCTATGTGTCTGTGCGGCACTTTCTCTCGGATTTGGAATCAAAGAGCATGGAGCGGATGAAGGCTGGTACGAGGGAGGGAGCATCTTTGTTGCTGTATTCATTGTCATTGTTGTCTCAGCTCTCAGTAACTTCAGACAAGAATTACAGTTCAACAAGTTGTCTAAAATCAGTAGCAACATCAAAGTTGAGGTATTTAGAGACAGACAAAGACAAGAAATCTCCATCTTTGATATTGTAGTTGGAGATGTTGTGGTTCTGAACATGGGAGATCAAATACCTGCAGACGGATTGTTCTTAGATGGGTATTCCTTACAAGTGGACGAGTCAAGCATGACGGGAGAGAGTGACCATGTTGAAGTTGATTCCACCGAGAACCCCTTCCTGTTCTCCGGTACAAAGGTGGTAGATGGCCATGCTCGAATGCTCGTCACATCAGTTGGGATGAACACCGCATGGGGAGAAATGATGAGCTCAATATCGCAGGACTCAAATGAAAGAACACCATTACAAGCAAGGCTTGACAAACTAACCTCTTCTATTGGAAAGGTGGGCCTTGCAGTTGCCTTACTAGTTCTTGTGGTCTTGTTGATTCGTTACTTCACTGGTAACACAAAAGATGAGTATGGACATACGGAGTATAGTGGTAGCAACAAAGACATAGATAGTGTGTTGAGCGGAGTAGTTCGCATTGTTTCCGCTGCAGTCACTATTGTGGTTGTGGCAATCCCGGAAGGTCTGCCCTTGGCCGTCACATTAACACTTGCTTACTCAATGAAAAGAATGATGAAAGATCAGGCTATGGTCAGAAAACTACAAGCTTGTGAGACGATGGGATCAGCAACCGTTATTTGTACTGACAAAACAGGAACTTTGACAAAAAATGAAATGGAAGTGACTAAATTTTGTCTTGGCCAAGAAGAAATTGAAAAAGATAATTCAATCACACCAAATGTTTGTGAATTATTTCATCAAGGAGTTGGCTTGAACACGACCGGAAGTGTTTACATACCTCTTTTAGGATCAAAACCTGAAATTTCTGGTAGTCCAACAGAGAAAGCAATTCTCTATTGGGCCGTTTCGGATTTGGGTATGGACATGGAAAAGTTGAAGCCTAGATATGACATTCTTCATGTTGAAACGTTCAACTCAGATAAGAAACGAAGTGGGGTTCTTATACAAAAGAAGGATGACAACACCATCCATGTGCACTGGAAAGGAGCTGCTGAGATGATAGTTGCAATGTGCTCGAGTTATTATGAAACCAACGGAACTATAAAGTCCCTGAATGAAGAAGGGAGGTCAAAAATTGAGAATATAATCCAAGCCATGGCAGCTAAAAGCCTCCGGTGCATTGCTTTTGCTCATACTCAAATTTTAGAAAAAGAAATTGAATATAGCGACGATAACAAGACCCACCCAAAGCTGAAAGAAGATGGATTATTTTTGCTAGGGGTAGTTGGTCTCAAAGATCCATGCCGGGCAGGAGTCACCGATGCTGTGAGAATTTGCATGTCTGCAGGCGTGCAAATCAAAATGATTACTGGAGACAATGTTTTCACAGCTAAAGCTATAGCTACAGAATGTGGAATACTAAAGACTGAGGACGCAACAAATGGTGATCAAGTGATAGAAGGAGCAGAATTCCGAAACTACACACACGAAGAGAGAATGAAAAAGGTCAATCAAATTCGCGTGATGGCAAGATCTTCCCCGTTCGACAAGCTATTGATGGTGCAGTGTTTGAGGCAGAAAGGACATGTGGTTGCAGTCACAGGAGATGGCACAAATGATGCTCCTGCACTGAAAGAAGCTGATATAGGACTTTCCATGGGAATCCAAGGTACTGAGGTGGCAAAAGAAAGCTCAGACATCATCATCTTGGACGACAACTTCAATTCTGTGGCCACAGTTTTGAGGTGGGGGCGATGTGTTTATAACAATATACAGAAGTTCATCCAGTTTCAACTAACTGTTAACGTCGCAGCCCTAGTAATTAACTTTATCGCAGCAGTTTCATCTGGTGATGTTCCCCTAACAGCTGTTCAATTGCTGTGGGTGAATCTCATCATGGACACACTAGGCGCTCTCGCCCTTGCTACCGAGCGGCCGACAGATGAGCTAATGCAGAAGAAGCCTGTAGGTCGAAGTGCACCGCTCATAACCAATATTATGTGGAGAAACCTTCTATGCCAAGCTCTATATCAAATAGCCATCCTCTTAACCTTGCAGTTCAGTGGCGATTCCATCTTCAATGTCACTGATGCGGTGAATGATACTATGATTTTCAACACGTTCGTACTCTGTCAAATTTTCAACGAGTTCAACTCAAGGAGCATGGAGAAGCAGAATGTATTCAAAGGTATTCACAGGAACAAACTGTTTATGGGCATTGTGGGGACAACCATCGTTCTTCAATTTGTGATGGTGGAGTTCCTGAAAAAGTTTGCAGATACAGAGAGGTTGACTTTGTTGCAATGGGTAACTTGTATCGGAATTGCAGCTGTATCATGGCCAATTGGTTGGATTGTGAAGTTGATGCCAGTTCCAGATGAACCATTCTTCAAAACTATCAAGAAGTTACTCAGATTCAAAAGGAATGTGTCCAGGTTCCAGAGCTCGGGGACTAGGCCAAGAAGTCTGGTACCTGAGCTCAGAGCTTTAGGTGTGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1156

Amino Acids

128.1

Weight (kDa)

6.39

Isoelectric Point (pI)

35.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	205 - 273	1.6e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	325 - 424	4e-23	P-type ATPase actuator domain
Cation_ATPase	PF13246	607 - 759	4.6e-29	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	686 - 870	1.7e-11	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	941 - 1110	3.2e-47	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63380
fragaria_vesca	FvH4_2g22900
malus_domestica	MD11G1278100.v1.1
prunus_persica	Prupe.8G234200_v2.0.a1 Prupe.8G234200_v2.0.a1
pyrus_communis	pycom11g24580
rosa_chinensis	RchiOBHm_Chr6g0289831
rosa_laevigata	RLG00000012264
rosa_multiflora	Rmu_sc0032573.1_g000001 Rmu_ssc0000314.1_g000015
rosa_roxburghii	Rroxscaffold_4G00321460 Rroxscaffold_7G00177520 Rroxscaffold_7G00178050
rosa_rugosa	Rorug06G0207300
rosa_samantha	Rh6AG318800
rosa_wichuraiana	Rw6G027500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2730
Acc36I	ACCTGC	1 cut(s)	1081
Acc65I	GGTACC	1 cut(s)	3436
AccB1I	GGYRCC	1 cut(s)	3436
AccB7I	CCANNNNNTGG	1 cut(s)	2122
AccBSI	CCGCTC	4 cut(s)	848, 1470, 2899, 2950
AccI	GTMKAC	1 cut(s)	201
AccII	CGCG	1 cut(s)	2487
AclI	AACGTT	1 cut(s)	1934
AclWI	GGATC	4 cut(s)	787, 1636, 1822, 2258
AcoI	YGGCCR	7 cut(s)	633, 1204, 1539, 1705, 2697, 2900, 3305
AcsI	RAATTY	9 cut(s)	551, 666, 1694, 1827, 2166, 2298, 2435, 2481, 3102
AcuI	CTGAAG	1 cut(s)	910
AfaI	GTAC	7 cut(s)	573, 863, 1189, 1648, 2638, 3093, 3438
AfiI	CCNNNNNNNGG	8 cut(s)	475, 662, 729, 743, 1079, 1811, 2122, 3412
AflIII	ACRYGT	3 cut(s)	109, 2551, 3084
AhlI	ACTAGT	1 cut(s)	1363
AjiI	CACGTC	2 cut(s)	121, 481
AjnI	CCWGG	2 cut(s)	149, 3398
AleI	CACNNNNGTG	1 cut(s)	1505
Alw21I	GWGCWC	6 cut(s)	1268, 2004, 2042, 2947, 3413, 3447
Alw26I	GTCTC	6 cut(s)	162, 913, 987, 1613, 2261, 2334
Alw44I	GTGCAC	2 cut(s)	2000, 2943
AlwI	GGATC	4 cut(s)	787, 1636, 1822, 2258
Ama87I	CYCGRG	3 cut(s)	443, 2041, 3412
ApaLI	GTGCAC	2 cut(s)	2000, 2943
ApoI	RAATTY	9 cut(s)	551, 666, 1694, 1827, 2166, 2298, 2435, 2481, 3102
Asp700I	GAANNNNTTC	3 cut(s)	1172, 1758, 3146
Asp718I	GGTACC	1 cut(s)	3436
AspLEI	GCGC	2 cut(s)	354, 2879
AspS9I	GGNCC	3 cut(s)	439, 1345, 1866
AsuC2I	CCSGG	2 cut(s)	1523, 2273
AsuHPI	GGTGA	8 cut(s)	307, 322, 592, 2275, 2423, 2828, 2863, 3073
AvaI	CYCGRG	3 cut(s)	443, 2041, 3412
AvaII	GGWCC	1 cut(s)	439
BaeGI	GKGCMC	2 cut(s)	2004, 2947
BaeI	ACNNNNGTAYC	4 cut(s)	1179, 1212, 2628, 2661
BalI	TGGCCA	5 cut(s)	635, 1206, 1707, 2699, 3307
BanI	GGYRCC	1 cut(s)	3436
BanII	GRGCYC	3 cut(s)	1268, 3413, 3447
Bbv12I	GWGCWC	6 cut(s)	1268, 2004, 2042, 2947, 3413, 3447
BceAI	ACGGC	3 cut(s)	513, 1526, 1853
BcgI	CGANNNNNNTGC	4 cut(s)	375, 409, 2022, 2056
BciT130I	CCWGG	2 cut(s)	151, 3400
BciVI	GTATCC	1 cut(s)	157
BclI	TGATCA	3 cut(s)	211, 556, 2413
BcnI	CCSGG	2 cut(s)	1523, 2273
BcoDI	GTCTC	6 cut(s)	162, 913, 987, 1613, 2261, 2334
BcuI	ACTAGT	1 cut(s)	1363
BfaI	CTAG	6 cut(s)	1364, 1908, 2243, 2783, 2873, 3420
BfmI	CTRYAG	7 cut(s)	187, 1074, 1494, 2310, 2364, 2370, 2931
BfoI	RGCGCY	1 cut(s)	2880
BfuAI	ACCTGC	1 cut(s)	1081
BfuI	GTATCC	1 cut(s)	157
BglI	GCCNNNNNGGC	1 cut(s)	101
BglII	AGATCT	2 cut(s)	447, 2498
Bme1390I	CCNGG	4 cut(s)	151, 1523, 2273, 3400
Bme18I	GGWCC	1 cut(s)	439
BmeT110I	CYCGRG	3 cut(s)	443, 2041, 3412
BmgBI	CACGTC	2 cut(s)	121, 481
BmgT120I	GGNCC	3 cut(s)	439, 1345, 1866
BmiI	GGNNCC	3 cut(s)	441, 3404, 3438
BmrFI	CCNGG	4 cut(s)	151, 1523, 2273, 3400
BmsI	GCATC	6 cut(s)	211, 885, 2278, 2575, 3046, 3321
BplI	GAGNNNNNCTC	2 cut(s)	3104, 3136
BpmI	CTGGAG	2 cut(s)	636, 2358
Bpu10I	CCTNAGC	1 cut(s)	3441
BpuEI	CTTGAG	2 cut(s)	149, 3106
BpuMI	CCSGG	2 cut(s)	1523, 2273
BsaAI	YACGTR	1 cut(s)	110
BsaI	GGTCTC	1 cut(s)	2261
BsaJI	CCNNGG	4 cut(s)	1536, 2121, 2622, 2632
BsaWI	WCCGGW	3 cut(s)	1184, 1787, 2139
BsaXI	ACNNNNNCTCC	4 cut(s)	1029, 1059, 2331, 2361
Bsc4I	CCNNNNNNNGG	8 cut(s)	475, 662, 729, 743, 1079, 1811, 2122, 3412
Bse118I	RCCGGY	1 cut(s)	62
Bse1I	ACTGG	7 cut(s)	184, 480, 1402, 2009, 2341, 2752, 3335
Bse3DI	GCAATG	4 cut(s)	393, 2040, 2145, 3275
BseBI	CCWGG	2 cut(s)	151, 3400
BseDI	CCNNGG	4 cut(s)	1536, 2121, 2622, 2632
BseGI	GGATG	7 cut(s)	96, 856, 1242, 1987, 1992, 2748, 3006
BseLI	CCNNNNNNNGG	8 cut(s)	475, 662, 729, 743, 1079, 1811, 2122, 3412
BseMI	GCAATG	4 cut(s)	393, 2040, 2145, 3275
BseNI	ACTGG	7 cut(s)	184, 480, 1402, 2009, 2341, 2752, 3335
BseRI	GAGGAG	3 cut(s)	35, 296, 633
BseSI	GKGCMC	2 cut(s)	2004, 2947
BseX3I	CGGCCG	1 cut(s)	2900
BsgI	GTGCAG	3 cut(s)	456, 2549, 2577
Bsh1236I	CGCG	1 cut(s)	2487
Bsh1285I	CGRYCG	2 cut(s)	1788, 2903
BshNI	GGYRCC	1 cut(s)	3436
BsiEI	CGRYCG	2 cut(s)	1788, 2903
BsiHKAI	GWGCWC	6 cut(s)	1268, 2004, 2042, 2947, 3413, 3447
BsiHKCI	CYCGRG	3 cut(s)	443, 2041, 3412
BsiSI	CCGG	6 cut(s)	63, 1185, 1523, 1788, 2140, 2272
BslFI	GGGAC	4 cut(s)	661, 2059, 3205, 3430
BslI	CCNNNNNNNGG	8 cut(s)	475, 662, 729, 743, 1079, 1811, 2122, 3412
BsmAI	GTCTC	6 cut(s)	162, 913, 987, 1613, 2261, 2334
BsmBI	CGTCTC	2 cut(s)	162, 1613
BsmFI	GGGAC	4 cut(s)	661, 2059, 3205, 3430
BsmI	GAATGC	1 cut(s)	1221
Bso31I	GGTCTC	1 cut(s)	2261
BsoBI	CYCGRG	3 cut(s)	443, 2041, 3412
Bsp1286I	GDGCHC	6 cut(s)	1268, 2004, 2042, 2947, 3413, 3447
Bsp19I	CCATGG	2 cut(s)	2121, 2622
BspFNI	CGCG	1 cut(s)	2487
BspHI	TCATGA	1 cut(s)	214
BspLI	GGNNCC	3 cut(s)	441, 3404, 3438
BspMAI	CTGCAG	3 cut(s)	1078, 1498, 2314
BspMI	ACCTGC	1 cut(s)	1081
BspPI	GGATC	4 cut(s)	787, 1636, 1822, 2258
BspT107I	GGYRCC	1 cut(s)	3436
BspTNI	GGTCTC	1 cut(s)	2261
BsrBI	CCGCTC	4 cut(s)	848, 1470, 2899, 2950
BsrDI	GCAATG	4 cut(s)	393, 2040, 2145, 3275
BsrFI	RCCGGY	1 cut(s)	62
BsrI	ACTGG	7 cut(s)	184, 480, 1402, 2009, 2341, 2752, 3335
BssAI	RCCGGY	1 cut(s)	62
BssECI	CCNNGG	4 cut(s)	1536, 2121, 2622, 2632
BssT1I	CCWWGG	4 cut(s)	1536, 2121, 2622, 2632
Bst2UI	CCWGG	2 cut(s)	151, 3400
Bst4CI	ACNGT	6 cut(s)	191, 942, 1639, 2704, 2767, 3174
Bst6I	CTCTTC	2 cut(s)	1333, 2451
BstAPI	GCANNNNNTGC	1 cut(s)	374
BstBAI	YACGTR	1 cut(s)	110
BstC8I	GCNNGC	3 cut(s)	7, 2314, 2318
BstDSI	CCRYGG	2 cut(s)	2121, 2622
BstENI	CCTNNNNNAGG	3 cut(s)	727, 741, 1809
BstF5I	GGATG	7 cut(s)	96, 856, 1242, 1987, 1992, 2748, 3006
BstFNI	CGCG	1 cut(s)	2487
BstH2I	RGCGCY	1 cut(s)	2880
BstHHI	GCGC	2 cut(s)	354, 2879
BstMAI	GTCTC	6 cut(s)	162, 913, 987, 1613, 2261, 2334
BstMCI	CGRYCG	2 cut(s)	1788, 2903
BstMWI	GCNNNNNNNGC	8 cut(s)	101, 230, 365, 374, 383, 2126, 2142, 2926
BstNI	CCWGG	2 cut(s)	151, 3400
BstNSI	RCATGY	2 cut(s)	2308, 2555
BstSCI	CCNGG	4 cut(s)	149, 1521, 2271, 3398
BstSFI	CTRYAG	7 cut(s)	187, 1074, 1494, 2310, 2364, 2370, 2931
BstSLI	GKGCMC	2 cut(s)	2004, 2947
BstUI	CGCG	1 cut(s)	2487
BstX2I	RGATCY	3 cut(s)	447, 2263, 2498
BstXI	CCANNNNNNTGG	1 cut(s)	3315
BstYI	RGATCY	3 cut(s)	447, 2263, 2498
BstZI	CGGCCG	1 cut(s)	2900
BsuI	GTATCC	1 cut(s)	157
BtgI	CCRYGG	2 cut(s)	2121, 2622
BtgZI	GCGATG	1 cut(s)	2734
BtrI	CACGTC	2 cut(s)	121, 481
BtsCI	GGATG	7 cut(s)	96, 856, 1242, 1987, 1992, 2748, 3006
BtsI	GCAGTG	4 cut(s)	19, 28, 492, 2537
BveI	ACCTGC	1 cut(s)	1081
Cac8I	GCNNGC	3 cut(s)	7, 2314, 2318
CciI	TCATGA	1 cut(s)	214
CfoI	GCGC	2 cut(s)	354, 2879
Cfr10I	RCCGGY	1 cut(s)	62
Cfr13I	GGNCC	3 cut(s)	439, 1345, 1866
CseI	GACGC	2 cut(s)	776, 2408
Csp6I	GTAC	7 cut(s)	572, 862, 1188, 1647, 2637, 3092, 3437
CspCI	CAANNNNNGTGG	4 cut(s)	625, 660, 2828, 2863
CviQI	GTAC	7 cut(s)	572, 862, 1188, 1647, 2637, 3092, 3437
DraI	TTTAAA	1 cut(s)	774
EaeI	YGGCCR	7 cut(s)	633, 1204, 1539, 1705, 2697, 2900, 3305
EagI	CGGCCG	1 cut(s)	2900
Eam1104I	CTCTTC	2 cut(s)	1333, 2451
EarI	CTCTTC	2 cut(s)	1333, 2451
EciI	GGCGGA	1 cut(s)	103
Ecl136II	GAGCTC	3 cut(s)	1266, 3411, 3445
EclXI	CGGCCG	1 cut(s)	2900
Eco130I	CCWWGG	4 cut(s)	1536, 2121, 2622, 2632
Eco24I	GRGCYC	3 cut(s)	1268, 3413, 3447
Eco31I	GGTCTC	1 cut(s)	2261
Eco32I	GATATC	1 cut(s)	508
Eco47I	GGWCC	1 cut(s)	439
Eco52I	CGGCCG	1 cut(s)	2900
Eco53kI	GAGCTC	3 cut(s)	1266, 3411, 3445
Eco57I	CTGAAG	1 cut(s)	910
Eco88I	CYCGRG	3 cut(s)	443, 2041, 3412
EcoICRI	GAGCTC	3 cut(s)	1266, 3411, 3445
EcoNI	CCTNNNNNAGG	3 cut(s)	727, 741, 1809
EcoO109I	RGGNCCY	1 cut(s)	439
EcoRI	GAATTC	2 cut(s)	666, 2435
EcoRII	CCWGG	2 cut(s)	149, 3398
EcoRV	GATATC	1 cut(s)	508
EcoT14I	CCWWGG	4 cut(s)	1536, 2121, 2622, 2632
EcoT38I	GRGCYC	3 cut(s)	1268, 3413, 3447
ErhI	CCWWGG	4 cut(s)	1536, 2121, 2622, 2632
Esp3I	CGTCTC	2 cut(s)	162, 1613
FalI	AAGNNNNNCTT	2 cut(s)	1296, 1328
FaqI	GGGAC	4 cut(s)	661, 2059, 3205, 3430
FbaI	TGATCA	3 cut(s)	211, 556, 2413
FblI	GTMKAC	1 cut(s)	201
FokI	GGATG	7 cut(s)	103, 863, 1249, 1979, 1994, 2735, 2993
FriOI	GRGCYC	3 cut(s)	1268, 3413, 3447
FspBI	CTAG	6 cut(s)	1364, 1908, 2243, 2783, 2873, 3420
GlaI	GCGC	2 cut(s)	353, 2878
GsuI	CTGGAG	2 cut(s)	636, 2358
HaeII	RGCGCY	1 cut(s)	2880
HapII	CCGG	6 cut(s)	63, 1185, 1523, 1788, 2140, 2272
HgaI	GACGC	2 cut(s)	776, 2408
HhaI	GCGC	2 cut(s)	354, 2879
Hin6I	GCGC	2 cut(s)	352, 2877
HinP1I	GCGC	2 cut(s)	352, 2877
HincII	GTYRAC	4 cut(s)	202, 691, 2770, 3258
HindII	GTYRAC	4 cut(s)	202, 691, 2770, 3258
HindIII	AAGCTT	1 cut(s)	1611
HpaI	GTTAAC	1 cut(s)	2770
HpaII	CCGG	6 cut(s)	63, 1185, 1523, 1788, 2140, 2272
HphI	GGTGA	8 cut(s)	307, 322, 592, 2275, 2423, 2828, 2863, 3073
Hpy188III	TCNNGA	6 cut(s)	215, 615, 3229, 3341, 3367, 3406
Hpy99I	CGWCG	4 cut(s)	53, 770, 2198, 2777
HpyCH4III	ACNGT	6 cut(s)	191, 942, 1639, 2704, 2767, 3174
HpyCH4IV	ACGT	6 cut(s)	109, 120, 480, 1934, 2772, 3086
HpyF10VI	GCNNNNNNNGC	8 cut(s)	101, 230, 365, 374, 383, 2126, 2142, 2926
HpySE526I	ACGT	6 cut(s)	109, 120, 480, 1934, 2772, 3086
HspAI	GCGC	2 cut(s)	352, 2877
KpnI	GGTACC	1 cut(s)	3440
Ksp22I	TGATCA	3 cut(s)	211, 556, 2413
KspAI	GTTAAC	1 cut(s)	2770
LmnI	GCTCC	8 cut(s)	48, 309, 845, 873, 2012, 2429, 2593, 3126
LweI	GCATC	6 cut(s)	211, 885, 2278, 2575, 3046, 3321
MaeI	CTAG	6 cut(s)	1364, 1908, 2243, 2783, 2873, 3420
MaeII	ACGT	6 cut(s)	109, 120, 480, 1934, 2772, 3086
MbiI	CCGCTC	4 cut(s)	848, 1470, 2899, 2950
MfeI	CAATTG	2 cut(s)	2840, 3309
MflI	RGATCY	3 cut(s)	447, 2263, 2498
MhlI	GDGCHC	6 cut(s)	1268, 2004, 2042, 2947, 3413, 3447
MlsI	TGGCCA	5 cut(s)	635, 1206, 1707, 2699, 3307
MluNI	TGGCCA	5 cut(s)	635, 1206, 1707, 2699, 3307
MlyI	GAGTC	6 cut(s)	11, 561, 620, 1127, 1274, 2289
MmeI	TCCRAC	3 cut(s)	1015, 1865, 3296
Mox20I	TGGCCA	5 cut(s)	635, 1206, 1707, 2699, 3307
MroXI	GAANNNNTTC	3 cut(s)	1172, 1758, 3146
MscI	TGGCCA	5 cut(s)	635, 1206, 1707, 2699, 3307
MseI	TTAA	5 cut(s)	773, 1550, 2769, 2790, 3014
MslI	CAYNNNNRTG	4 cut(s)	219, 675, 1505, 1997
Msp20I	TGGCCA	5 cut(s)	635, 1206, 1707, 2699, 3307
MspA1I	CMGCKG	3 cut(s)	1493, 2834, 3296
MspI	CCGG	6 cut(s)	63, 1185, 1523, 1788, 2140, 2272
MspR9I	CCNGG	4 cut(s)	151, 1523, 2273, 3400
MunI	CAATTG	2 cut(s)	2840, 3309
Mva1269I	GAATGC	1 cut(s)	1221
MvaI	CCWGG	2 cut(s)	151, 3400
MvnI	CGCG	1 cut(s)	2487
MwoI	GCNNNNNNNGC	8 cut(s)	101, 230, 365, 374, 383, 2126, 2142, 2926
NciI	CCSGG	2 cut(s)	1523, 2273
NcoI	CCATGG	2 cut(s)	2121, 2622
NlaIV	GGNNCC	3 cut(s)	441, 3404, 3438
NmeAIII	GCCGAG	1 cut(s)	236
NmuCI	GTSAC	9 cut(s)	14, 1139, 1222, 1498, 1543, 1687, 2281, 2563, 3049
NspI	RCATGY	2 cut(s)	2308, 2555
OliI	CACNNNNGTG	1 cut(s)	1505
PaeR7I	CTCGAG	1 cut(s)	2041
PagI	TCATGA	1 cut(s)	214
PciI	ACATGT	1 cut(s)	2551
PctI	GAATGC	1 cut(s)	1221
PdmI	GAANNNNTTC	3 cut(s)	1172, 1758, 3146
PfeI	GAWTC	8 cut(s)	278, 831, 1157, 1384, 2628, 2854, 3035, 3381
PflMI	CCANNNNNTGG	1 cut(s)	2122
PfoI	TCCNGGA	2 cut(s)	149, 1521
PleI	GAGTC	6 cut(s)	11, 561, 619, 1126, 1274, 2288
PpsI	GAGTC	6 cut(s)	11, 561, 619, 1126, 1274, 2288
Ppu21I	YACGTR	1 cut(s)	110
PpuMI	RGGWCCY	1 cut(s)	439
PscI	ACATGT	1 cut(s)	2551
PsiI	TTATAA	1 cut(s)	2730
Psp124BI	GAGCTC	3 cut(s)	1268, 3413, 3447
Psp1406I	AACGTT	1 cut(s)	1934
Psp5II	RGGWCCY	1 cut(s)	439
Psp6I	CCWGG	2 cut(s)	149, 3398
PspGI	CCWGG	2 cut(s)	149, 3398
PspN4I	GGNNCC	3 cut(s)	441, 3404, 3438
PspPI	GGNCC	3 cut(s)	439, 1345, 1866
PspPPI	RGGWCCY	1 cut(s)	439
PspXI	VCTCGAGB	1 cut(s)	2041
PstI	CTGCAG	3 cut(s)	1078, 1498, 2314
PsuI	RGATCY	3 cut(s)	447, 2263, 2498
PvuII	CAGCTG	2 cut(s)	2834, 3296
RsaI	GTAC	7 cut(s)	573, 863, 1189, 1648, 2638, 3093, 3438
RsaNI	GTAC	7 cut(s)	572, 862, 1188, 1647, 2637, 3092, 3437
RseI	CAYNNNNRTG	4 cut(s)	219, 675, 1505, 1997
SacI	GAGCTC	3 cut(s)	1268, 3413, 3447
SalI	GTCGAC	1 cut(s)	200
SaqAI	TTAA	5 cut(s)	773, 1550, 2769, 2790, 3014
Sau96I	GGNCC	3 cut(s)	439, 1345, 1866
SchI	GAGTC	6 cut(s)	11, 561, 620, 1127, 1274, 2289
ScrFI	CCNGG	4 cut(s)	151, 1523, 2273, 3400
SduI	GDGCHC	6 cut(s)	1268, 2004, 2042, 2947, 3413, 3447
SfaNI	GCATC	6 cut(s)	211, 885, 2278, 2575, 3046, 3321
SfcI	CTRYAG	7 cut(s)	187, 1074, 1494, 2310, 2364, 2370, 2931
Sfr274I	CTCGAG	1 cut(s)	2041
SinI	GGWCC	1 cut(s)	439
SlaI	CTCGAG	1 cut(s)	2041
SmiMI	CAYNNNNRTG	4 cut(s)	219, 675, 1505, 1997
SmlI	CTYRAG	3 cut(s)	128, 2041, 3121
SmoI	CTYRAG	3 cut(s)	128, 2041, 3121
SpeI	ACTAGT	1 cut(s)	1363
SspI	AATATT	1 cut(s)	2962
SspMI	CTAG	6 cut(s)	1364, 1908, 2243, 2783, 2873, 3420
SstI	GAGCTC	3 cut(s)	1268, 3413, 3447
StyD4I	CCNGG	4 cut(s)	149, 1521, 2271, 3398
StyI	CCWWGG	4 cut(s)	1536, 2121, 2622, 2632
TaaI	ACNGT	6 cut(s)	191, 942, 1639, 2704, 2767, 3174
TaiI	ACGT	6 cut(s)	112, 123, 483, 1937, 2775, 3089
TaqI	TCGA	8 cut(s)	48, 201, 309, 765, 1213, 2042, 2511, 2938
TatI	WGTACW	1 cut(s)	1646
TauI	GCSGC	3 cut(s)	36, 812, 2902
TfiI	GAWTC	8 cut(s)	278, 831, 1157, 1384, 2628, 2854, 3035, 3381
Tru1I	TTAA	5 cut(s)	773, 1550, 2769, 2790, 3014
Tru9I	TTAA	5 cut(s)	773, 1550, 2769, 2790, 3014
TseFI	GTSAC	9 cut(s)	14, 1139, 1222, 1498, 1543, 1687, 2281, 2563, 3049
Tsp45I	GTSAC	9 cut(s)	14, 1139, 1222, 1498, 1543, 1687, 2281, 2563, 3049
TspGWI	ACGGA	3 cut(s)	1095, 1442, 2076
Van91I	CCANNNNNTGG	1 cut(s)	2122
VneI	GTGCAC	2 cut(s)	2000, 2943
VpaK11BI	GGWCC	1 cut(s)	439
XagI	CCTNNNNNAGG	3 cut(s)	727, 741, 1809
XapI	RAATTY	9 cut(s)	551, 666, 1694, 1827, 2166, 2298, 2435, 2481, 3102
XceI	RCATGY	2 cut(s)	2308, 2555
XhoI	CTCGAG	1 cut(s)	2041
XmiI	GTMKAC	1 cut(s)	201
XmnI	GAANNNNTTC	3 cut(s)	1172, 1758, 3146
XspI	CTAG	6 cut(s)	1364, 1908, 2243, 2783, 2873, 3420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.