Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0014891

Physical Location & Seq

Genomic Coordinates Reverse (-)

17731120 .. 17736064

Gene Structure 4945 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00178050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2892 bp

ATGGTGAGCAGCAAGAATTCACCTTATGACTGTGGCCAGTTGATAGTTGACATCACCACTACCACTTTCACCAGAGCGAAGAAGAGGTGGCGCATGGCGTATGCTGCCTTGTATGCTGTAAGGGTGATGCTATCACTTCCCAAAGAGCTTATGTACAAGAGGAGCAACTACAAGGACTACTCTGAGATCATCGAGTATGGATCTTTCGGTGGTTTCCAAGGTCTCAAAGAAAGCCGGCGGGAGAAGGGAGATTCAAACTCTGGTCTCGAGCATGAGGCTAAAAGCTCTTCACCACTGGAGCTACAATCCCCTGCACACGATGAGTCTCGTTCTTACACTTCCCTGGAGGTGAAACCAAGCACTTCCCCTGATGAAACTGGTCACTATAGTTCTGTCCCCAACAACATTGACCATGCAGGGCTCGAAAAAATGGTGAAGGAAAAAGACTCGGCTGCTCTTGGAAAGTTAGGAGGAGTTGCTGGCATAACCACTTCTCTTAAAACAAATATTGAGTATGGGCTACCTGGCGACGACGCAGAAATCAACAAACGCCGTGTACTATTCGGCTCAAATACATACCCAAAGCCGCCTTCAAAAGGGCTCCTCTATTTTGTGCTGGACGCTCTGAAAGACATGACGATTCTGATTCTATGTGTCTGTGCAGCACTTTCTCTTGCATTTGGAATCAAAGAGTATGGAGTAGAGGAAGGCTGGTATGAAGGTGGAAGTATCTTTGTAGCTGTAATCATTGTCATTGTTGTCTCAGCTCTCAGTAACTTCAGACAGGAAATCCAATTCGACAAGCTGTCTAAAATAAGCAGCAACATCAACGTTGAGGTATTTAGAGACAGACAAAGACGAGAAATCTCCATCTTTGATGTTGTGGTTGGAGATGTCGTCTTCCTTAACATTGGAGATCAAATTCCTGCAGATGGGGTGTTCTTAGATGGCTATTCATTTCAAGTGGACGAGTCCAGCATGACCGGGGAGAGCGACTATGTAGAAGTTGATTCCAGAAAGAATCCCTTCCTCTTGTCTGGTGCAAAGGTGGCTGATGGTTATGGTAGAATGCTTGTCACATCAGTTGGGATGAACACTGCTTGGGGTGAAATGATGAGCTCAATTTCGCAGGAATCAAATGTAAGAACTCCATTACAAGCTCGGCTTGACAAACTTACCTCTTCTATTGGAAAGGTAGGCCTTGCAGTTGCTTTCTTAGTTCTTCTAGTTTTGTTGATTCGTTATTTCACTGGCAATACAAAAGATAAGAATGGCAATAAGGAGTATATTGGTAGCAAAACAAAAACAGATGATGTGTTGAATGCAATAGTACGGATTGTATCTGCTGCAGTTACTATTGTGGTGGTGGCAATCCCTGAAGGCTTGCCACTGGCTGTCACACTAACACTTGCTTACTCTATGAAAAGAATGATGAAAGATCACGCAATGGTTAGAAAACTATCGGCTTGTGAGACAATGGGGTCAGCAACCGTTATCTGTACTGACAAAACAGGAACTTTGACACTAAACCAAATGAAAGTAACTCAGTTTTGGGTAGGCCAAGAATATATTGAAGAAGATAATTCATCCATCACACCAAATGTTCGTGAGTTATTCTATCAAGGAGTTGGTTTGAACACAACTGGGAGTGTTTTCATGCCTCTTGCAGGATCCAAACCAGAAATCTCTGGCAGCCCAACAGAAAAAGCAATACTCTATTGGGCTGTTTTAGATTTGGGCATGGACATGCAAGAACTGAGGCCCAGGTATGACATTCTTCATGTTGAAACATTTAATTCAGACAAGAAACGAAGTGGGGTTCTTATAAAGAAAAAAGATGATAGCACCGTCCACGTGCACTGGAAAGGAGCTGCTGAGATGATAGTTGCAACATGCTCGAGTTATTATGATACCAATGGAACTGCAAAGCCCTTGGATGTCGATACAAGGTCTAAAGTTGAGAAAATAATCCAAGGCATGGCAGCTAGTAGCCTCCGATGCATTGCTTTCGCTCATGCCCAAATTTCAGAGGAAGAAGTTGAATACAGTGATGATGACAAGAAGCACCAAAAGCTCAAAGAAGATGGGCTGATCTTGCTAGGGATAGTCGGCCTCAAAGATCCATGTCGACCAGGGGTCGTCCATGCTGTGGGAGTTTGCAAGGATGCGGGTGTGGACATCAAAATGATAACTGGAGACAATATTTTCACAGCAAAAGCTATAGCTACAGAATGTGGAATACTAAAGAATGAGAACGAGGTAAAGGATGGACAAGTTGTAGAAGGAGTCGAATTTCGAAACTACACTGATGCAGAGAGAATGCAGAAGGTTGATAACATCCGTGTGATGGCAAGGTCCTCTCCATTTGACAAGCTTCTAATGGTACAGTGTTTGAAGGAGAAAGGTCATGTGGTTGCAGTCACTGGTGATGGTACGAATGATGCACCTGCATTGAAAGAAGCTGATATAGGACTTTCCATGGGCATCCAAGGAACTGAAGTGGCAAAAGAAAGCTCGGATATCATCATCTTGGATGACAACTTCAATTCTGTGGCCACAGTTTTGAGATGGGGGAGATGTGTTTATAACAATATACAGAAATTCATCCAATTTCAACTAACTGTTAATGTTGCAGCTCTAGTAATCAACTTTATTGCAGCAGTTTCAGCTGGTGAAGTTCCCCTAACAGCAGTTCAATTACTTTGGGTGAATCTCATCATGGACACGCTAGGTGCTCTGGCTCTTGCTACGGAGCGGCCAACTAATGAGCTAATGGAGAGGCAGCCCGTGGGTCGGACCGCTCCACTCATAACCAATATTATGTGGAGGAATCTTTTTAGCCCAAGCTCTATATCAAATAGCTGTTCTCTTGATCTTGCAGTTCCAGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

963

Amino Acids

105.48

Weight (kDa)

5.6

Isoelectric Point (pI)

37.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	157 - 224	8.4e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	277 - 375	2e-21	P-type ATPase actuator domain
Cation_ATPase	PF13246	559 - 711	5e-31	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	680 - 822	5.7e-12	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	893 - 946	1.2e-16	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63380
fragaria_vesca	FvH4_2g22900
malus_domestica	MD11G1278100.v1.1
prunus_persica	Prupe.8G234200_v2.0.a1 Prupe.8G234200_v2.0.a1
pyrus_communis	pycom11g24580
rosa_chinensis	RchiOBHm_Chr6g0289831
rosa_laevigata	RLG00000012264
rosa_multiflora	Rmu_sc0032573.1_g000001 Rmu_ssc0000314.1_g000015
rosa_roxburghii	Rroxscaffold_4G00321460 Rroxscaffold_7G00177520 Rroxscaffold_7G00178050
rosa_rugosa	Rorug06G0207300
rosa_samantha	Rh6AG318800
rosa_wichuraiana	Rw6G027500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1826, 2586
AarI	CACCTGC	1 cut(s)	2455
AasI	GACNNNNNNGTC	1 cut(s)	1480
Acc36I	ACCTGC	1 cut(s)	2455
AccB7I	CCANNNNNTGG	2 cut(s)	1978, 2149
AccBSI	CCGCTC	2 cut(s)	2755, 2801
AccI	GTMKAC	1 cut(s)	2128
AciI	CCGC	5 cut(s)	238, 587, 2168, 2755, 2799
AclI	AACGTT	1 cut(s)	831
AclWI	GGATC	4 cut(s)	208, 1665, 1678, 2114
AcoI	YGGCCR	3 cut(s)	34, 2553, 2756
AcsI	RAATTY	5 cut(s)	16, 921, 2022, 2291, 2600
AcuI	CTGAAG	3 cut(s)	763, 1398, 2517
AcvI	CACGTG	1 cut(s)	1855
AfaI	GTAC	6 cut(s)	155, 558, 1332, 1501, 2385, 2434
AfiI	CCNNNNNNNGG	7 cut(s)	596, 932, 1667, 1978, 2149, 2347, 2793
AhdI	GACNNNNNGTC	2 cut(s)	805, 2135
AjnI	CCWGG	5 cut(s)	342, 523, 1763, 2131, 2884
AjuI	GAANNNNNNNTTGG	2 cut(s)	574, 606
Alw21I	GWGCWC	3 cut(s)	1121, 1860, 2737
Alw26I	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
Alw44I	GTGCAC	1 cut(s)	1856
AlwI	GGATC	4 cut(s)	208, 1665, 1678, 2114
AlwNI	CAGNNNCTG	1 cut(s)	2423
Ama87I	CYCGRG	2 cut(s)	266, 1897
AoxI	GGCC	7 cut(s)	34, 1198, 1558, 1760, 2110, 2553, 2756
ApaLI	GTGCAC	1 cut(s)	1856
ApoI	RAATTY	5 cut(s)	16, 921, 2022, 2291, 2600
Asp700I	GAANNNNTTC	1 cut(s)	1025
AspLEI	GCGC	1 cut(s)	93
AspS9I	GGNCC	3 cut(s)	1761, 2355, 2796
AsuC2I	CCSGG	1 cut(s)	985
AsuII	TTCGAA	1 cut(s)	2296
AvaI	CYCGRG	2 cut(s)	266, 1897
AvaII	GGWCC	2 cut(s)	2355, 2796
BaeGI	GKGCMC	1 cut(s)	1860
BalI	TGGCCA	2 cut(s)	36, 2555
BamHI	GGATCC	1 cut(s)	1670
BanII	GRGCYC	3 cut(s)	423, 603, 1121
BbrPI	CACGTG	1 cut(s)	1855
BbsI	GAAGAC	1 cut(s)	892
Bbv12I	GWGCWC	3 cut(s)	1121, 1860, 2737
BceAI	ACGGC	1 cut(s)	537
BcgI	CGANNNNNNTGC	4 cut(s)	1878, 1912, 1990, 2024
BciT130I	CCWGG	5 cut(s)	344, 525, 1765, 2133, 2886
BcnI	CCSGG	1 cut(s)	985
BcoDI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
BfaI	CTAG	5 cut(s)	1226, 1986, 2099, 2639, 2729
BfmI	CTRYAG	5 cut(s)	385, 927, 1347, 2220, 2226
BfuAI	ACCTGC	1 cut(s)	2455
Bme1390I	CCNGG	6 cut(s)	344, 525, 985, 1765, 2133, 2886
Bme18I	GGWCC	2 cut(s)	2355, 2796
BmeRI	GACNNNNNGTC	2 cut(s)	805, 2135
BmeT110I	CYCGRG	2 cut(s)	266, 1897
BmgT120I	GGNCC	3 cut(s)	1761, 2355, 2796
BmiI	GGNNCC	2 cut(s)	602, 1672
BmrFI	CCNGG	6 cut(s)	344, 525, 985, 1765, 2133, 2886
BmrI	ACTGGG	1 cut(s)	1653
BmsI	GCATC	6 cut(s)	117, 1988, 2155, 2299, 2431, 2493
BmuI	ACTGGG	1 cut(s)	1653
BpiI	GAAGAC	1 cut(s)	892
BpmI	CTGGAG	3 cut(s)	317, 365, 2214
Bpu14I	TTCGAA	1 cut(s)	2296
BpuMI	CCSGG	1 cut(s)	985
BsaAI	YACGTR	1 cut(s)	1855
BsaI	GGTCTC	2 cut(s)	227, 269
BsaXI	ACNNNNNCTCC	2 cut(s)	882, 912
Bsc4I	CCNNNNNNNGG	7 cut(s)	596, 932, 1667, 1978, 2149, 2347, 2793
Bse118I	RCCGGY	1 cut(s)	234
Bse1I	ACTGG	9 cut(s)	37, 300, 382, 1255, 1395, 1648, 1865, 2197, 2428
Bse3DI	GCAATG	2 cut(s)	1452, 2001
BseBI	CCWGG	5 cut(s)	344, 525, 1765, 2133, 2886
BseGI	GGATG	9 cut(s)	1095, 1586, 1942, 2170, 2272, 2337, 2484, 2539, 2604
BseLI	CCNNNNNNNGG	7 cut(s)	596, 932, 1667, 1978, 2149, 2347, 2793
BseMI	GCAATG	2 cut(s)	1452, 2001
BseMII	CTCAG	6 cut(s)	174, 777, 784, 1559, 1748, 1866
BseNI	ACTGG	9 cut(s)	37, 300, 382, 1255, 1395, 1648, 1865, 2197, 2428
BseRI	GAGGAG	3 cut(s)	175, 486, 593
BseSI	GKGCMC	1 cut(s)	1860
BsgI	GTGCAG	2 cut(s)	297, 681
BshFI	GGCC	7 cut(s)	36, 1200, 1560, 1762, 2112, 2555, 2758
BsiHKAI	GWGCWC	3 cut(s)	1121, 1860, 2737
BsiHKCI	CYCGRG	2 cut(s)	266, 1897
BsiSI	CCGG	2 cut(s)	235, 984
BslFI	GGGAC	1 cut(s)	380
BslI	CCNNNNNNNGG	7 cut(s)	596, 932, 1667, 1978, 2149, 2347, 2793
BsmAI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
BsmFI	GGGAC	1 cut(s)	380
BsmI	GAATGC	3 cut(s)	1074, 1327, 2325
BsnI	GGCC	7 cut(s)	36, 1200, 1560, 1762, 2112, 2555, 2758
Bso31I	GGTCTC	2 cut(s)	227, 269
BsoBI	CYCGRG	2 cut(s)	266, 1897
Bsp119I	TTCGAA	1 cut(s)	2296
Bsp1286I	GDGCHC	5 cut(s)	423, 603, 1121, 1860, 2737
Bsp1407I	TGTACA	1 cut(s)	153
Bsp143I	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
Bsp19I	CCATGG	1 cut(s)	2478
BspACI	CCGC	5 cut(s)	238, 587, 2168, 2755, 2799
BspANI	GGCC	7 cut(s)	36, 1200, 1560, 1762, 2112, 2555, 2758
BspCNI	CTCAG	6 cut(s)	175, 776, 783, 1558, 1749, 1867
BspLI	GGNNCC	2 cut(s)	602, 1672
BspMAI	CTGCAG	2 cut(s)	931, 1351
BspMI	ACCTGC	1 cut(s)	2455
BspPI	GGATC	4 cut(s)	208, 1665, 1678, 2114
BspQI	GCTCTTC	1 cut(s)	292
BspT104I	TTCGAA	1 cut(s)	2296
BspTNI	GGTCTC	2 cut(s)	227, 269
BsrBI	CCGCTC	2 cut(s)	2755, 2801
BsrDI	GCAATG	2 cut(s)	1452, 2001
BsrFI	RCCGGY	1 cut(s)	234
BsrGI	TGTACA	1 cut(s)	153
BsrI	ACTGG	9 cut(s)	37, 300, 382, 1255, 1395, 1648, 1865, 2197, 2428
BssAI	RCCGGY	1 cut(s)	234
BssMI	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
BssT1I	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
Bst2UI	CCWGG	5 cut(s)	344, 525, 1765, 2133, 2886
Bst4CI	ACNGT	7 cut(s)	32, 1492, 1849, 2048, 2388, 2560, 2623
Bst6I	CTCTTC	3 cut(s)	77, 292, 1186
BstAUI	TGTACA	1 cut(s)	153
BstBAI	YACGTR	1 cut(s)	1855
BstBI	TTCGAA	1 cut(s)	2296
BstC8I	GCNNGC	3 cut(s)	236, 481, 1385
BstDEI	CTNAG	8 cut(s)	183, 763, 770, 943, 1216, 1545, 1757, 1875
BstDSI	CCRYGG	2 cut(s)	2478, 2787
BstENI	CCTNNNNNAGG	2 cut(s)	594, 1665
BstF5I	GGATG	9 cut(s)	1095, 1586, 1942, 2170, 2272, 2337, 2484, 2539, 2604
BstHHI	GCGC	1 cut(s)	93
BstKTI	GATC	8 cut(s)	189, 203, 919, 1441, 1673, 2094, 2122, 2875
BstMAI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
BstMBI	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
BstMWI	GCNNNNNNNGC	7 cut(s)	104, 113, 1049, 1998, 2071, 2095, 2666
BstNI	CCWGG	5 cut(s)	344, 525, 1765, 2133, 2886
BstNSI	RCATGY	2 cut(s)	1750, 1896
BstSCI	CCNGG	6 cut(s)	342, 523, 983, 1763, 2131, 2884
BstSFI	CTRYAG	5 cut(s)	385, 927, 1347, 2220, 2226
BstSLI	GKGCMC	1 cut(s)	1860
BstV2I	GAAGAC	1 cut(s)	892
BstX2I	RGATCY	3 cut(s)	200, 1670, 2119
BstYI	RGATCY	3 cut(s)	200, 1670, 2119
BsuRI	GGCC	7 cut(s)	36, 1200, 1560, 1762, 2112, 2555, 2758
BtgI	CCRYGG	2 cut(s)	2478, 2787
BtsCI	GGATG	9 cut(s)	1095, 1586, 1942, 2170, 2272, 2337, 2484, 2539, 2604
BtsI	GCAGTG	1 cut(s)	1095
BtsIMutI	CAGTG	9 cut(s)	293, 1095, 1248, 1388, 1858, 2053, 2304, 2393, 2421
BveI	ACCTGC	1 cut(s)	2455
Cac8I	GCNNGC	3 cut(s)	236, 481, 1385
CaiI	CAGNNNCTG	1 cut(s)	2423
CfoI	GCGC	1 cut(s)	93
Cfr10I	RCCGGY	1 cut(s)	234
Cfr13I	GGNCC	3 cut(s)	1761, 2355, 2796
CpoI	CGGWCCG	1 cut(s)	2796
CseI	GACGC	2 cut(s)	542, 629
Csp6I	GTAC	6 cut(s)	154, 557, 1331, 1500, 2384, 2433
CspI	CGGWCCG	1 cut(s)	2796
CviQI	GTAC	6 cut(s)	154, 557, 1331, 1500, 2384, 2433
DdeI	CTNAG	8 cut(s)	183, 763, 770, 943, 1216, 1545, 1757, 1875
DpnI	GATC	8 cut(s)	188, 202, 918, 1440, 1672, 2093, 2121, 2874
DpnII	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
DrdI	GACNNNNNNGTC	1 cut(s)	1480
DriI	GACNNNNNGTC	2 cut(s)	805, 2135
DseDI	GACNNNNNNGTC	1 cut(s)	1480
EaeI	YGGCCR	3 cut(s)	34, 2553, 2756
Eam1104I	CTCTTC	3 cut(s)	77, 292, 1186
Eam1105I	GACNNNNNGTC	2 cut(s)	805, 2135
EarI	CTCTTC	3 cut(s)	77, 292, 1186
Ecl136II	GAGCTC	1 cut(s)	1119
Eco130I	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
Eco147I	AGGCCT	1 cut(s)	1200
Eco24I	GRGCYC	3 cut(s)	423, 603, 1121
Eco31I	GGTCTC	2 cut(s)	227, 269
Eco32I	GATATC	1 cut(s)	2521
Eco47I	GGWCC	2 cut(s)	2355, 2796
Eco53kI	GAGCTC	1 cut(s)	1119
Eco57I	CTGAAG	3 cut(s)	763, 1398, 2517
Eco72I	CACGTG	1 cut(s)	1855
Eco88I	CYCGRG	2 cut(s)	266, 1897
EcoICRI	GAGCTC	1 cut(s)	1119
EcoNI	CCTNNNNNAGG	2 cut(s)	594, 1665
EcoO109I	RGGNCCY	1 cut(s)	2355
EcoRI	GAATTC	1 cut(s)	16
EcoRII	CCWGG	5 cut(s)	342, 523, 1763, 2131, 2884
EcoRV	GATATC	1 cut(s)	2521
EcoT14I	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
EcoT22I	ATGCAT	1 cut(s)	2003
EcoT38I	GRGCYC	3 cut(s)	423, 603, 1121
ErhI	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
FalI	AAGNNNNNCTT	4 cut(s)	1010, 1042, 1149, 1181
FaqI	GGGAC	1 cut(s)	380
FauI	CCCGC	2 cut(s)	231, 2161
FblI	GTMKAC	1 cut(s)	2128
FokI	GGATG	9 cut(s)	1102, 1573, 1949, 2177, 2279, 2324, 2471, 2546, 2591
FriOI	GRGCYC	3 cut(s)	423, 603, 1121
FspBI	CTAG	5 cut(s)	1226, 1986, 2099, 2639, 2729
GlaI	GCGC	1 cut(s)	92
GsuI	CTGGAG	3 cut(s)	317, 365, 2214
HaeIII	GGCC	7 cut(s)	36, 1200, 1560, 1762, 2112, 2555, 2758
HapII	CCGG	2 cut(s)	235, 984
HgaI	GACGC	2 cut(s)	542, 629
HhaI	GCGC	1 cut(s)	93
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HincII	GTYRAC	2 cut(s)	49, 2129
HindII	GTYRAC	2 cut(s)	49, 2129
HindIII	AAGCTT	1 cut(s)	2372
HpaII	CCGG	2 cut(s)	235, 984
Hpy166II	GTNNAC	7 cut(s)	49, 557, 967, 1852, 1858, 2129, 2176
Hpy188I	TCNGA	9 cut(s)	184, 627, 645, 782, 1801, 1997, 2029, 2518, 2796
Hpy188III	TCNNGA	4 cut(s)	266, 1014, 1607, 2870
Hpy8I	GTNNAC	7 cut(s)	49, 557, 967, 1852, 1858, 2129, 2176
Hpy99I	CGWCG	2 cut(s)	533, 536
HpyCH4III	ACNGT	7 cut(s)	32, 1492, 1849, 2048, 2388, 2560, 2623
HpyCH4IV	ACGT	2 cut(s)	831, 1854
HpyF10VI	GCNNNNNNNGC	7 cut(s)	104, 113, 1049, 1998, 2071, 2095, 2666
HpyF3I	CTNAG	8 cut(s)	183, 763, 770, 943, 1216, 1545, 1757, 1875
HpySE526I	ACGT	2 cut(s)	831, 1854
HspAI	GCGC	1 cut(s)	91
KroI	GCCGGC	1 cut(s)	234
KroNI	GCCGGC	1 cut(s)	236
Kzo9I	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
LguI	GCTCTTC	1 cut(s)	292
LmnI	GCTCC	6 cut(s)	162, 298, 606, 1868, 2752, 2806
LweI	GCATC	6 cut(s)	117, 1988, 2155, 2299, 2431, 2493
MaeI	CTAG	5 cut(s)	1226, 1986, 2099, 2639, 2729
MaeII	ACGT	2 cut(s)	831, 1854
MaeIII	GTNAC	7 cut(s)	380, 773, 1075, 1351, 1396, 1540, 2419
MalI	GATC	8 cut(s)	188, 202, 918, 1440, 1672, 2093, 2121, 2874
MbiI	CCGCTC	2 cut(s)	2755, 2801
MboI	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
MflI	RGATCY	3 cut(s)	200, 1670, 2119
MhlI	GDGCHC	5 cut(s)	423, 603, 1121, 1860, 2737
MlsI	TGGCCA	2 cut(s)	36, 2555
MluNI	TGGCCA	2 cut(s)	36, 2555
MlyI	GAGTC	4 cut(s)	332, 440, 980, 2295
MmeI	TCCRAC	2 cut(s)	868, 2774
Mox20I	TGGCCA	2 cut(s)	36, 2555
Mph1103I	ATGCAT	1 cut(s)	2003
MroNI	GCCGGC	1 cut(s)	234
MroXI	GAANNNNTTC	1 cut(s)	1025
MscI	TGGCCA	2 cut(s)	36, 2555
MseI	TTAA	4 cut(s)	498, 906, 1794, 2625
MslI	CAYNNNNRTG	3 cut(s)	1745, 2183, 2342
Msp20I	TGGCCA	2 cut(s)	36, 2555
MspA1I	CMGCKG	1 cut(s)	2669
MspI	CCGG	2 cut(s)	235, 984
MspR9I	CCNGG	6 cut(s)	344, 525, 985, 1765, 2133, 2886
Mva1269I	GAATGC	3 cut(s)	1074, 1327, 2325
MvaI	CCWGG	5 cut(s)	344, 525, 1765, 2133, 2886
MwoI	GCNNNNNNNGC	7 cut(s)	104, 113, 1049, 1998, 2071, 2095, 2666
NaeI	GCCGGC	1 cut(s)	236
NciI	CCSGG	1 cut(s)	985
NcoI	CCATGG	1 cut(s)	2478
NdeII	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
NgoMIV	GCCGGC	1 cut(s)	234
NlaIV	GGNNCC	2 cut(s)	602, 1672
NmeAIII	GCCGAG	2 cut(s)	428, 1141
NmuCI	GTSAC	4 cut(s)	380, 1075, 1396, 2419
NsiI	ATGCAT	1 cut(s)	2003
NspI	RCATGY	2 cut(s)	1750, 1896
NspV	TTCGAA	1 cut(s)	2296
PaeR7I	CTCGAG	2 cut(s)	266, 1897
PaqCI	CACCTGC	1 cut(s)	2455
PceI	AGGCCT	1 cut(s)	1200
PciSI	GCTCTTC	1 cut(s)	292
PctI	GAATGC	3 cut(s)	1074, 1327, 2325
PdiI	GCCGGC	1 cut(s)	236
PdmI	GAANNNNTTC	1 cut(s)	1025
PflMI	CCANNNNNTGG	2 cut(s)	1978, 2149
PleI	GAGTC	4 cut(s)	331, 440, 979, 2294
PmaCI	CACGTG	1 cut(s)	1855
PmlI	CACGTG	1 cut(s)	1855
PpsI	GAGTC	4 cut(s)	331, 440, 979, 2294
Ppu21I	YACGTR	1 cut(s)	1855
PpuMI	RGGWCCY	1 cut(s)	2355
PsiI	TTATAA	2 cut(s)	1826, 2586
Psp124BI	GAGCTC	1 cut(s)	1121
Psp1406I	AACGTT	1 cut(s)	831
Psp5II	RGGWCCY	1 cut(s)	2355
Psp6I	CCWGG	5 cut(s)	342, 523, 1763, 2131, 2884
PspCI	CACGTG	1 cut(s)	1855
PspGI	CCWGG	5 cut(s)	342, 523, 1763, 2131, 2884
PspN4I	GGNNCC	2 cut(s)	602, 1672
PspPI	GGNCC	3 cut(s)	1761, 2355, 2796
PspPPI	RGGWCCY	1 cut(s)	2355
PspXI	VCTCGAGB	1 cut(s)	1897
PstI	CTGCAG	2 cut(s)	931, 1351
PstNI	CAGNNNCTG	1 cut(s)	2423
PsuI	RGATCY	3 cut(s)	200, 1670, 2119
PvuII	CAGCTG	1 cut(s)	2669
RsaI	GTAC	6 cut(s)	155, 558, 1332, 1501, 2385, 2434
RsaNI	GTAC	6 cut(s)	154, 557, 1331, 1500, 2384, 2433
RseI	CAYNNNNRTG	3 cut(s)	1745, 2183, 2342
Rsr2I	CGGWCCG	1 cut(s)	2796
RsrII	CGGWCCG	1 cut(s)	2796
SacI	GAGCTC	1 cut(s)	1121
SalI	GTCGAC	1 cut(s)	2127
SapI	GCTCTTC	1 cut(s)	292
SaqAI	TTAA	4 cut(s)	498, 906, 1794, 2625
Sau3AI	GATC	8 cut(s)	186, 200, 916, 1438, 1670, 2091, 2119, 2872
Sau96I	GGNCC	3 cut(s)	1761, 2355, 2796
SchI	GAGTC	4 cut(s)	332, 440, 980, 2295
ScrFI	CCNGG	6 cut(s)	344, 525, 985, 1765, 2133, 2886
SduI	GDGCHC	5 cut(s)	423, 603, 1121, 1860, 2737
SfaNI	GCATC	6 cut(s)	117, 1988, 2155, 2299, 2431, 2493
SfcI	CTRYAG	5 cut(s)	385, 927, 1347, 2220, 2226
Sfr274I	CTCGAG	2 cut(s)	266, 1897
SfuI	TTCGAA	1 cut(s)	2296
SinI	GGWCC	2 cut(s)	2355, 2796
SlaI	CTCGAG	2 cut(s)	266, 1897
SmiMI	CAYNNNNRTG	3 cut(s)	1745, 2183, 2342
SmlI	CTYRAG	2 cut(s)	266, 1897
SmoI	CTYRAG	2 cut(s)	266, 1897
SseBI	AGGCCT	1 cut(s)	1200
SsiI	CCGC	5 cut(s)	238, 587, 2168, 2755, 2799
SspI	AATATT	3 cut(s)	508, 2203, 2818
SspMI	CTAG	5 cut(s)	1226, 1986, 2099, 2639, 2729
SstI	GAGCTC	1 cut(s)	1121
StuI	AGGCCT	1 cut(s)	1200
StyD4I	CCNGG	6 cut(s)	342, 523, 983, 1763, 2131, 2884
StyI	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
TaaI	ACNGT	7 cut(s)	32, 1492, 1849, 2048, 2388, 2560, 2623
TaiI	ACGT	2 cut(s)	834, 1857
TaqI	TCGA	9 cut(s)	192, 267, 423, 798, 1898, 1941, 2128, 2289, 2296
TatI	WGTACW	3 cut(s)	153, 556, 1499
TauI	GCSGC	2 cut(s)	589, 2758
Tru1I	TTAA	4 cut(s)	498, 906, 1794, 2625
Tru9I	TTAA	4 cut(s)	498, 906, 1794, 2625
TscAI	CASTG	9 cut(s)	300, 1102, 1255, 1395, 1865, 2053, 2311, 2393, 2428
TseFI	GTSAC	4 cut(s)	380, 1075, 1396, 2419
Tsp45I	GTSAC	4 cut(s)	380, 1075, 1396, 2419
TspGWI	ACGGA	3 cut(s)	1348, 2330, 2765
TspRI	CASTG	9 cut(s)	300, 1102, 1255, 1395, 1865, 2053, 2311, 2393, 2428
Van91I	CCANNNNNTGG	2 cut(s)	1978, 2149
VneI	GTGCAC	1 cut(s)	1856
VpaK11BI	GGWCC	2 cut(s)	2355, 2796
XagI	CCTNNNNNAGG	2 cut(s)	594, 1665
XapI	RAATTY	5 cut(s)	16, 921, 2022, 2291, 2600
XceI	RCATGY	2 cut(s)	1750, 1896
XhoI	CTCGAG	2 cut(s)	266, 1897
XmiI	GTMKAC	1 cut(s)	2128
XmnI	GAANNNNTTC	1 cut(s)	1025
XspI	CTAG	5 cut(s)	1226, 1986, 2099, 2639, 2729
Zsp2I	ATGCAT	1 cut(s)	2003

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.