Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0014891

Physical Location & Seq

Genomic Coordinates Forward (+)

51803070 .. 51809882

Gene Structure 6813 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG318800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3294 bp

ATGGTGAGCAGCAAGAATTCACCTTATGATTGTGGCCAGTTGATAGTTGACATCACCACTACCACTTTTACCAGAGCGAAGAAGAGGTGGCGCATGGCTTATGCAGCCTTGTATGCTGTAAGGGTGATGCTATCACTTCCCAAAGAGCTTATGTACAAGAGGAGCAACTACAAGGAATACTCAGAGATCTCGGAGTACGGATCTTTCGGTGGTTTCCAAGGTCTCAAAGAAAGCCAGCGGGAGAAGGGAGATTCGAACTCTGGTCTCGAGCATGAGGCTAAAAACTCCTCACCACTGGAGCTACAATCCCCTGCACATGATGAGTCTCGTTCTTACACTTCCCTGGAGGTGAAACCAAGCACTTCCCCTGATGAAACTGGTCACTATAGTTCTGTCCCCATCAACATTGACCATGCAGGGCTCGAAAAAATGGTGAAGGAAAAAGACTCGGCTGCTCTTGGAAAGTTTGGAGGAGTTACTGGCATAACCACTTCTCTTAAAACAAATCCTGAGTATGGGCTACCTGGCAACGACGCAGAAATCAACAAACGCCGTGAACTATTCGGCTCAAATACATACCCAAAGCCGCCTTCAAAAGGGCTCCTCTATTTTGTGCTGGACGCTCTGAAAGACATGACGATTCTGATTCTATGTGTCTGTGCAGCACTTTCTCTTGCATTTGGAATCAAAGAGTATGGAGTAGAGGAAGGCTGGTATGAAGGTGGAAGTATCTTTGTAGCTGTAATCATTGTCATTGTTGTCTCAGCTCTCAGTAACTTCAGACAGGAAATCCAATTCGACAAACTGTCTAAAATAAGCAGCAACATCAACGTTGAGGTATTTAGAGACAGACAAAGACGAGAAATCTCCATCTTTGATGTTGTGGTTGGAGATGTCATCTTCCTTAACATTGGAGATCAAATTCCTGCAGACGGGGTGTTCTTAGATGGCTATTCATTTCAAGTGGACGAGTCCAGCATGACCGGGGAGAGCGACTATGTAGAAGTTGATTCCAGAAGGCATCCCTTCCTCTTGTCTGGTGCAAAGGTGGCTGATGGTTATGGTAGAATGCTTGTCACATCAGTTGGGATGAACACTGCTTGGGGCGAAATGATGAGCTCAATTTCGCAGGAATCAAATGTAAGAACTCCATTACAAGCTCGGCTTGACAAACTTACCTCTTCTATTGGAAAGGTAGGCCTTGCAGTTGCTTTCTTAGTTCTTCTAGTTTTGTTGATTCGTTATTTTACTGGCAATACAAAAGATGAGAATGGCAATAAGGAGTATATTGGTAGCAAAACCAAAACAGATGATGTGTTGAATGCAGTAGTACGAATTGTATCTGCTGCAGTCACTATTGTGGTGGTGGCAATTCCTGAAGGCTTGCCACTGGCTGTCACACTAACACTTGCTTACTCTATGAAAAGAATGATGAAAGATCAGGCAATGGTTAGAAAACTATCGGCTTGTGAGACAATGGGGTCAGCAACCGTTATCTGTACTGACAAAACAGGAACTCTGACACTAAACCAAATGAAAGTAACTAAGTTTTGGGTAGGCCAAGAATCTATTGCAGAAGATAATTTATCCATCGCACCAAATGTTCGTGAGTTATTCTATCAAGGAGTTGCACCAAATGTTCGTGAGTTATTCCATCAAGGAGTTGGTTTGAACACAACTGGGAGTGTTTTCATGCCTCTTGCAGGATCCAAACCAGAAATCTCTGGCAGCCCAACAGAAAAAGCAATACTCTATTGGGCTGTTTTAGATTTGGGAATGGACATGCAAGAACTGAAGCCCAGGTATGATATTCTTCATGTTGAAACATTTAATTCAGACAAGAAACGAAGTGGGGTTCTTATAAAGAAAAAAGATGATAACACCATCCATGTGCACTGGAAAGGAGCTGCTGAGATGATAGTTGCAACATGCTCGAGTTATTATGATACCAATGGAACTGTAAAGCCCTTGGATGTAGACACAAGGTCTAAACTTGAGAAAATAATCCAAGGCATGGCAGCTAGTAGCCTCCGATGCATTGCTTTCGCTCATGCCCAAATTTCAGAGGAAGAAGTTGAATACAGTGATGATCACAAGACACACCAAAAGCTCAAAGAAGATGGGCTGATCTTGCTAGGGATAGTTGGCCTCAAAGATCCATGTCGACCAGGGGTCATCAATGCTGTGAGAATTTGCAAGGCTGCAGGTGTGGACATCAAAATGATAACTGGAGACAATATTTTCACAGCAAAAGCTATAGCTACAGAATGTGGAATACTAAAGAAAGAGAACGAGGTACAGGGCGGACAAGTTGTAGAAGGAGTCGAATTTCGAAACTACACTGACGCAGAGAGAAGGCAGAAGGTTGATAACATCCGTGTGATGGCAAGGTCCTCCCCATTTGACAAGCTTTTAATGGTACAGTGTTTGAAGGAGAAAGGTCATGTGGTTGCAGTCACTGGTGATGGTACGAATGATGCACCTGCATTGAAAGAAGCTGATATAGGACTTTCCATGGGCATCCAAGGAACTGAGGTGGCAAAAGAAAGCTCGGATATCATCATCTTGGATGACAACTTCAATTCTGTGGCCACAGTTTTGAGATGGGGGAGATGTGTTTATAACAATATACAGAAATTCATCCAATTTCAACTAACTGTTAATGTTGCAGCTCTAGTAATCAACTTTATTGCAGCAGTTTCAGCTGGTGAAGTTCCCCTAACAGCAGTTCAATTACTTTGGGTGAATCTCATCATGGACACGCTAGGTGCTCTGGCTCTTGCTACGGAGCGGCCAACTAATGAGCTAATGGAGAGGCAGCCTGTGGGTCGGACCGCTCCACTCATAACCAATATTATGTGGAGGAATCTTTTAGCCCAAGCTCTATATCAAATAGCTGTTCTCTTGATCTTGCAGTTCCAGGGTGAGTCTATATTCAATGTCACTGCTGCGGTGAATGATACTCTGATTTTCAATACCTTTGTATTATGCCAAGTTTTCAACGAGTTCAATTCAAGAAGCATGGAGAAGAAGAATGTATTCAAAGGTATTCTCAGGAATAGGCTGTTTATTGGGATTGTGGGGATAACTATTGTTCTTCAGGTTGTGATGGTGGAGTTTCTGAAGAAATTTGCAGATACAGAAAAGTTGAATGTTCAGCAATGGGTAGTTTGTATCATGATTGCAGCTGTATCATGGCCAATTGGTTGGGTTGTGAAGTTCATACCTGTTTCAGAGGACCCATACTTCAGAATTATCAAAAGGTCACTCCTCAGATTAAAAAACATTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1097

Amino Acids

121.21

Weight (kDa)

6.44

Isoelectric Point (pI)

35.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	157 - 224	1.4e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	277 - 375	5.4e-21	P-type ATPase actuator domain
Cation_ATPase	PF13246	570 - 723	2.1e-30	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	693 - 834	9.4e-13	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	905 - 1075	4.1e-46	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63380
fragaria_vesca	FvH4_2g22900
malus_domestica	MD11G1278100.v1.1
prunus_persica	Prupe.8G234200_v2.0.a1 Prupe.8G234200_v2.0.a1
pyrus_communis	pycom11g24580
rosa_chinensis	RchiOBHm_Chr6g0289831
rosa_laevigata	RLG00000012264
rosa_multiflora	Rmu_sc0032573.1_g000001 Rmu_ssc0000314.1_g000015
rosa_roxburghii	Rroxscaffold_4G00321460 Rroxscaffold_7G00177520 Rroxscaffold_7G00178050
rosa_rugosa	Rorug06G0207300
rosa_samantha	Rh6AG318800
rosa_wichuraiana	Rw6G027500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1862, 2622
AarI	CACCTGC	2 cut(s)	2195, 2491
AasI	GACNNNNNNGTC	1 cut(s)	1480
Acc36I	ACCTGC	2 cut(s)	2195, 2491
AccB7I	CCANNNNNTGG	1 cut(s)	2014
AccBSI	CCGCTC	2 cut(s)	2791, 2837
AccI	GTMKAC	2 cut(s)	1977, 2164
AciI	CCGC	6 cut(s)	238, 587, 2304, 2791, 2835, 2951
AclI	AACGTT	1 cut(s)	831
AclWI	GGATC	4 cut(s)	208, 1701, 1714, 2150
AcoI	YGGCCR	4 cut(s)	34, 2589, 2792, 3197
AcsI	RAATTY	7 cut(s)	16, 921, 2058, 2190, 2327, 2636, 3128
AcuI	CTGAAG	6 cut(s)	763, 1398, 1814, 3083, 3143, 3232
AfaI	GTAC	7 cut(s)	155, 197, 1332, 1501, 2298, 2421, 2470
AfiI	CCNNNNNNNGG	6 cut(s)	515, 596, 932, 1703, 2014, 2383
AhdI	GACNNNNNGTC	3 cut(s)	805, 1984, 2171
AjnI	CCWGG	5 cut(s)	342, 523, 1799, 2167, 2919
AjuI	GAANNNNNNNTTGG	2 cut(s)	574, 606
AleI	CACNNNNGTG	1 cut(s)	1358
Alw21I	GWGCWC	3 cut(s)	1121, 1896, 2773
Alw26I	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2226
Alw44I	GTGCAC	1 cut(s)	1892
AlwI	GGATC	4 cut(s)	208, 1701, 1714, 2150
AlwNI	CAGNNNCTG	1 cut(s)	2459
Ama87I	CYCGRG	2 cut(s)	266, 1933
AoxI	GGCC	7 cut(s)	34, 1198, 1558, 2146, 2589, 2792, 3197
ApaLI	GTGCAC	1 cut(s)	1892
ApoI	RAATTY	7 cut(s)	16, 921, 2058, 2190, 2327, 2636, 3128
Asp700I	GAANNNNTTC	1 cut(s)	3038
AspLEI	GCGC	1 cut(s)	93
AspS9I	GGNCC	3 cut(s)	2391, 2832, 3238
AsuC2I	CCSGG	1 cut(s)	985
AsuII	TTCGAA	2 cut(s)	254, 2332
AvaI	CYCGRG	2 cut(s)	266, 1933
AvaII	GGWCC	3 cut(s)	2391, 2832, 3238
BaeGI	GKGCMC	1 cut(s)	1896
BalI	TGGCCA	3 cut(s)	36, 2591, 3199
BamHI	GGATCC	1 cut(s)	1706
BanII	GRGCYC	3 cut(s)	423, 603, 1121
Bbv12I	GWGCWC	3 cut(s)	1121, 1896, 2773
BceAI	ACGGC	1 cut(s)	537
BcgI	CGANNNNNNTGC	4 cut(s)	1914, 1948, 2026, 2060
BciT130I	CCWGG	5 cut(s)	344, 525, 1801, 2169, 2921
BclI	TGATCA	1 cut(s)	2089
BcnI	CCSGG	1 cut(s)	985
BcoDI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2226
BfaI	CTAG	5 cut(s)	1226, 2022, 2135, 2675, 2765
BfmI	CTRYAG	6 cut(s)	385, 927, 1347, 2202, 2256, 2262
BfuAI	ACCTGC	2 cut(s)	2195, 2491
BglII	AGATCT	1 cut(s)	186
Bme1390I	CCNGG	6 cut(s)	344, 525, 985, 1801, 2169, 2921
Bme18I	GGWCC	3 cut(s)	2391, 2832, 3238
BmeRI	GACNNNNNGTC	3 cut(s)	805, 1984, 2171
BmeT110I	CYCGRG	2 cut(s)	266, 1933
BmgT120I	GGNCC	3 cut(s)	2391, 2832, 3238
BmiI	GGNNCC	3 cut(s)	602, 1708, 3240
BmrFI	CCNGG	6 cut(s)	344, 525, 985, 1801, 2169, 2921
BmrI	ACTGGG	1 cut(s)	1689
BmsI	GCATC	5 cut(s)	117, 1030, 2024, 2467, 2529
BmuI	ACTGGG	1 cut(s)	1689
BpmI	CTGGAG	3 cut(s)	317, 365, 2250
Bpu14I	TTCGAA	2 cut(s)	254, 2332
BpuEI	CTTGAG	1 cut(s)	2015
BpuMI	CCSGG	1 cut(s)	985
BsaI	GGTCTC	2 cut(s)	227, 269
Bsc4I	CCNNNNNNNGG	6 cut(s)	515, 596, 932, 1703, 2014, 2383
Bse3DI	GCAATG	3 cut(s)	1452, 2037, 3167
BseBI	CCWGG	5 cut(s)	344, 525, 1801, 2169, 2921
BseGI	GGATG	8 cut(s)	1021, 1095, 1884, 1978, 2373, 2520, 2575, 2640
BseLI	CCNNNNNNNGG	6 cut(s)	515, 596, 932, 1703, 2014, 2383
BseMI	GCAATG	3 cut(s)	1452, 2037, 3167
BseMII	CTCAG	8 cut(s)	195, 501, 777, 784, 1902, 2523, 3067, 3286
BseRI	GAGGAG	5 cut(s)	175, 277, 486, 593, 3260
BseSI	GKGCMC	1 cut(s)	1896
BsgI	GTGCAG	2 cut(s)	297, 681
BshFI	GGCC	7 cut(s)	36, 1200, 1560, 2148, 2591, 2794, 3199
BsiHKAI	GWGCWC	3 cut(s)	1121, 1896, 2773
BsiHKCI	CYCGRG	2 cut(s)	266, 1933
BsiSI	CCGG	1 cut(s)	984
BslFI	GGGAC	1 cut(s)	380
BslI	CCNNNNNNNGG	6 cut(s)	515, 596, 932, 1703, 2014, 2383
BsmAI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2226
BsmFI	GGGAC	1 cut(s)	380
BsmI	GAATGC	2 cut(s)	1074, 1327
BsnI	GGCC	7 cut(s)	36, 1200, 1560, 2148, 2591, 2794, 3199
Bso31I	GGTCTC	2 cut(s)	227, 269
BsoBI	CYCGRG	2 cut(s)	266, 1933
Bsp119I	TTCGAA	2 cut(s)	254, 2332
Bsp1286I	GDGCHC	5 cut(s)	423, 603, 1121, 1896, 2773
Bsp1407I	TGTACA	1 cut(s)	153
Bsp143I	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
Bsp19I	CCATGG	1 cut(s)	2514
BspACI	CCGC	6 cut(s)	238, 587, 2304, 2791, 2835, 2951
BspANI	GGCC	7 cut(s)	36, 1200, 1560, 2148, 2591, 2794, 3199
BspCNI	CTCAG	8 cut(s)	194, 502, 776, 783, 1903, 2524, 3066, 3285
BspHI	TCATGA	1 cut(s)	3177
BspLI	GGNNCC	3 cut(s)	602, 1708, 3240
BspMAI	CTGCAG	3 cut(s)	931, 1351, 2206
BspMI	ACCTGC	2 cut(s)	2195, 2491
BspPI	GGATC	4 cut(s)	208, 1701, 1714, 2150
BspT104I	TTCGAA	2 cut(s)	254, 2332
BspTNI	GGTCTC	2 cut(s)	227, 269
BsrBI	CCGCTC	2 cut(s)	2791, 2837
BsrDI	GCAATG	3 cut(s)	1452, 2037, 3167
BsrGI	TGTACA	1 cut(s)	153
BssMI	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
BssT1I	CCWWGG	5 cut(s)	217, 1968, 2008, 2514, 2524
Bst2UI	CCWGG	5 cut(s)	344, 525, 1801, 2169, 2921
Bst4CI	ACNGT	7 cut(s)	807, 1492, 1960, 2084, 2424, 2596, 2659
Bst6I	CTCTTC	2 cut(s)	77, 1186
BstAUI	TGTACA	1 cut(s)	153
BstBI	TTCGAA	2 cut(s)	254, 2332
BstC8I	GCNNGC	2 cut(s)	236, 1385
BstDSI	CCRYGG	1 cut(s)	2514
BstENI	CCTNNNNNAGG	2 cut(s)	594, 1701
BstF5I	GGATG	8 cut(s)	1021, 1095, 1884, 1978, 2373, 2520, 2575, 2640
BstHHI	GCGC	1 cut(s)	93
BstKTI	GATC	9 cut(s)	189, 203, 919, 1441, 1709, 2092, 2130, 2158, 2910
BstMAI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2226
BstMBI	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
BstMWI	GCNNNNNNNGC	6 cut(s)	104, 113, 1049, 2034, 2131, 2702
BstNI	CCWGG	5 cut(s)	344, 525, 1801, 2169, 2921
BstNSI	RCATGY	2 cut(s)	1786, 1932
BstSCI	CCNGG	6 cut(s)	342, 523, 983, 1799, 2167, 2919
BstSFI	CTRYAG	6 cut(s)	385, 927, 1347, 2202, 2256, 2262
BstSLI	GKGCMC	1 cut(s)	1896
BstX2I	RGATCY	4 cut(s)	186, 200, 1706, 2155
BstXI	CCANNNNNNTGG	1 cut(s)	3207
BstYI	RGATCY	4 cut(s)	186, 200, 1706, 2155
BsuRI	GGCC	7 cut(s)	36, 1200, 1560, 2148, 2591, 2794, 3199
BtgI	CCRYGG	1 cut(s)	2514
BtgZI	GCGATG	1 cut(s)	1576
BtsCI	GGATG	8 cut(s)	1021, 1095, 1884, 1978, 2373, 2520, 2575, 2640
BtsI	GCAGTG	2 cut(s)	1095, 2943
BtsIMutI	CAGTG	9 cut(s)	293, 1095, 1388, 1894, 2089, 2340, 2429, 2457, 2943
BveI	ACCTGC	2 cut(s)	2195, 2491
Cac8I	GCNNGC	2 cut(s)	236, 1385
CaiI	CAGNNNCTG	1 cut(s)	2459
CciI	TCATGA	1 cut(s)	3177
CfoI	GCGC	1 cut(s)	93
Cfr13I	GGNCC	3 cut(s)	2391, 2832, 3238
CpoI	CGGWCCG	1 cut(s)	2832
CseI	GACGC	3 cut(s)	542, 629, 2354
Csp6I	GTAC	7 cut(s)	154, 196, 1331, 1500, 2297, 2420, 2469
CspI	CGGWCCG	1 cut(s)	2832
CviQI	GTAC	7 cut(s)	154, 196, 1331, 1500, 2297, 2420, 2469
DpnI	GATC	9 cut(s)	188, 202, 918, 1440, 1708, 2091, 2129, 2157, 2909
DpnII	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
DrdI	GACNNNNNNGTC	1 cut(s)	1480
DriI	GACNNNNNGTC	3 cut(s)	805, 1984, 2171
DseDI	GACNNNNNNGTC	1 cut(s)	1480
EaeI	YGGCCR	4 cut(s)	34, 2589, 2792, 3197
Eam1104I	CTCTTC	2 cut(s)	77, 1186
Eam1105I	GACNNNNNGTC	3 cut(s)	805, 1984, 2171
EarI	CTCTTC	2 cut(s)	77, 1186
EciI	GGCGGA	1 cut(s)	2319
Ecl136II	GAGCTC	1 cut(s)	1119
Eco130I	CCWWGG	5 cut(s)	217, 1968, 2008, 2514, 2524
Eco147I	AGGCCT	1 cut(s)	1200
Eco24I	GRGCYC	3 cut(s)	423, 603, 1121
Eco31I	GGTCTC	2 cut(s)	227, 269
Eco32I	GATATC	1 cut(s)	2557
Eco47I	GGWCC	3 cut(s)	2391, 2832, 3238
Eco53kI	GAGCTC	1 cut(s)	1119
Eco57I	CTGAAG	6 cut(s)	763, 1398, 1814, 3083, 3143, 3232
Eco88I	CYCGRG	2 cut(s)	266, 1933
EcoICRI	GAGCTC	1 cut(s)	1119
EcoNI	CCTNNNNNAGG	2 cut(s)	594, 1701
EcoO109I	RGGNCCY	2 cut(s)	2391, 3238
EcoRI	GAATTC	1 cut(s)	16
EcoRII	CCWGG	5 cut(s)	342, 523, 1799, 2167, 2919
EcoRV	GATATC	1 cut(s)	2557
EcoT14I	CCWWGG	5 cut(s)	217, 1968, 2008, 2514, 2524
EcoT22I	ATGCAT	1 cut(s)	2039
EcoT38I	GRGCYC	3 cut(s)	423, 603, 1121
ErhI	CCWWGG	5 cut(s)	217, 1968, 2008, 2514, 2524
FalI	AAGNNNNNCTT	2 cut(s)	1149, 1181
FaqI	GGGAC	1 cut(s)	380
FauI	CCCGC	1 cut(s)	231
FbaI	TGATCA	1 cut(s)	2089
FblI	GTMKAC	2 cut(s)	1977, 2164
FokI	GGATG	8 cut(s)	1008, 1102, 1871, 1985, 2360, 2507, 2582, 2627
FriOI	GRGCYC	3 cut(s)	423, 603, 1121
FspBI	CTAG	5 cut(s)	1226, 2022, 2135, 2675, 2765
GlaI	GCGC	1 cut(s)	92
GsuI	CTGGAG	3 cut(s)	317, 365, 2250
HaeIII	GGCC	7 cut(s)	36, 1200, 1560, 2148, 2591, 2794, 3199
HapII	CCGG	1 cut(s)	984
HgaI	GACGC	3 cut(s)	542, 629, 2354
HhaI	GCGC	1 cut(s)	93
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HincII	GTYRAC	2 cut(s)	49, 2165
HindII	GTYRAC	2 cut(s)	49, 2165
HindIII	AAGCTT	1 cut(s)	2408
HpaII	CCGG	1 cut(s)	984
Hpy166II	GTNNAC	7 cut(s)	49, 557, 967, 1894, 1978, 2165, 2212
Hpy8I	GTNNAC	7 cut(s)	49, 557, 967, 1894, 1978, 2165, 2212
Hpy99I	CGWCG	1 cut(s)	536
HpyCH4III	ACNGT	7 cut(s)	807, 1492, 1960, 2084, 2424, 2596, 2659
HpyCH4IV	ACGT	1 cut(s)	831
HpyF10VI	GCNNNNNNNGC	6 cut(s)	104, 113, 1049, 2034, 2131, 2702
HpySE526I	ACGT	1 cut(s)	831
HspAI	GCGC	1 cut(s)	91
Ksp22I	TGATCA	1 cut(s)	2089
Kzo9I	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
LmnI	GCTCC	6 cut(s)	162, 298, 606, 1904, 2788, 2842
LweI	GCATC	5 cut(s)	117, 1030, 2024, 2467, 2529
MaeI	CTAG	5 cut(s)	1226, 2022, 2135, 2675, 2765
MaeII	ACGT	1 cut(s)	831
MalI	GATC	9 cut(s)	188, 202, 918, 1440, 1708, 2091, 2129, 2157, 2909
MbiI	CCGCTC	2 cut(s)	2791, 2837
MboI	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
MfeI	CAATTG	1 cut(s)	3201
MflI	RGATCY	4 cut(s)	186, 200, 1706, 2155
MhlI	GDGCHC	5 cut(s)	423, 603, 1121, 1896, 2773
MlsI	TGGCCA	3 cut(s)	36, 2591, 3199
MluNI	TGGCCA	3 cut(s)	36, 2591, 3199
MlyI	GAGTC	5 cut(s)	332, 440, 980, 2331, 2936
MmeI	TCCRAC	2 cut(s)	868, 2810
Mox20I	TGGCCA	3 cut(s)	36, 2591, 3199
Mph1103I	ATGCAT	1 cut(s)	2039
MroXI	GAANNNNTTC	1 cut(s)	3038
MscI	TGGCCA	3 cut(s)	36, 2591, 3199
MseI	TTAA	6 cut(s)	498, 906, 1830, 2414, 2661, 3278
MslI	CAYNNNNRTG	4 cut(s)	1358, 1889, 2219, 2378
Msp20I	TGGCCA	3 cut(s)	36, 2591, 3199
MspA1I	CMGCKG	3 cut(s)	238, 2705, 3188
MspI	CCGG	1 cut(s)	984
MspR9I	CCNGG	6 cut(s)	344, 525, 985, 1801, 2169, 2921
MunI	CAATTG	1 cut(s)	3201
Mva1269I	GAATGC	2 cut(s)	1074, 1327
MvaI	CCWGG	5 cut(s)	344, 525, 1801, 2169, 2921
MwoI	GCNNNNNNNGC	6 cut(s)	104, 113, 1049, 2034, 2131, 2702
NciI	CCSGG	1 cut(s)	985
NcoI	CCATGG	1 cut(s)	2514
NdeII	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
NlaIV	GGNNCC	3 cut(s)	602, 1708, 3240
NmeAIII	GCCGAG	2 cut(s)	428, 1141
NmuCI	GTSAC	7 cut(s)	380, 1075, 1351, 1396, 2455, 2941, 3264
NsiI	ATGCAT	1 cut(s)	2039
NspI	RCATGY	2 cut(s)	1786, 1932
NspV	TTCGAA	2 cut(s)	254, 2332
OliI	CACNNNNGTG	1 cut(s)	1358
PaeR7I	CTCGAG	2 cut(s)	266, 1933
PagI	TCATGA	1 cut(s)	3177
PaqCI	CACCTGC	2 cut(s)	2195, 2491
PceI	AGGCCT	1 cut(s)	1200
PctI	GAATGC	2 cut(s)	1074, 1327
PdmI	GAANNNNTTC	1 cut(s)	3038
PflMI	CCANNNNNTGG	1 cut(s)	2014
PleI	GAGTC	5 cut(s)	331, 440, 979, 2330, 2935
PpsI	GAGTC	5 cut(s)	331, 440, 979, 2330, 2935
PpuMI	RGGWCCY	2 cut(s)	2391, 3238
PsiI	TTATAA	2 cut(s)	1862, 2622
Psp124BI	GAGCTC	1 cut(s)	1121
Psp1406I	AACGTT	1 cut(s)	831
Psp5II	RGGWCCY	2 cut(s)	2391, 3238
Psp6I	CCWGG	5 cut(s)	342, 523, 1799, 2167, 2919
PspGI	CCWGG	5 cut(s)	342, 523, 1799, 2167, 2919
PspN4I	GGNNCC	3 cut(s)	602, 1708, 3240
PspPI	GGNCC	3 cut(s)	2391, 2832, 3238
PspPPI	RGGWCCY	2 cut(s)	2391, 3238
PspXI	VCTCGAGB	1 cut(s)	1933
PstI	CTGCAG	3 cut(s)	931, 1351, 2206
PstNI	CAGNNNCTG	1 cut(s)	2459
PsuI	RGATCY	4 cut(s)	186, 200, 1706, 2155
PvuII	CAGCTG	2 cut(s)	2705, 3188
RsaI	GTAC	7 cut(s)	155, 197, 1332, 1501, 2298, 2421, 2470
RsaNI	GTAC	7 cut(s)	154, 196, 1331, 1500, 2297, 2420, 2469
RseI	CAYNNNNRTG	4 cut(s)	1358, 1889, 2219, 2378
Rsr2I	CGGWCCG	1 cut(s)	2832
RsrII	CGGWCCG	1 cut(s)	2832
SacI	GAGCTC	1 cut(s)	1121
SalI	GTCGAC	1 cut(s)	2163
SaqAI	TTAA	6 cut(s)	498, 906, 1830, 2414, 2661, 3278
Sau3AI	GATC	9 cut(s)	186, 200, 916, 1438, 1706, 2089, 2127, 2155, 2907
Sau96I	GGNCC	3 cut(s)	2391, 2832, 3238
SchI	GAGTC	5 cut(s)	332, 440, 980, 2331, 2936
ScrFI	CCNGG	6 cut(s)	344, 525, 985, 1801, 2169, 2921
SduI	GDGCHC	5 cut(s)	423, 603, 1121, 1896, 2773
SfaNI	GCATC	5 cut(s)	117, 1030, 2024, 2467, 2529
SfcI	CTRYAG	6 cut(s)	385, 927, 1347, 2202, 2256, 2262
Sfr274I	CTCGAG	2 cut(s)	266, 1933
SfuI	TTCGAA	2 cut(s)	254, 2332
SinI	GGWCC	3 cut(s)	2391, 2832, 3238
SlaI	CTCGAG	2 cut(s)	266, 1933
SmiMI	CAYNNNNRTG	4 cut(s)	1358, 1889, 2219, 2378
SmlI	CTYRAG	3 cut(s)	266, 1933, 1994
SmoI	CTYRAG	3 cut(s)	266, 1933, 1994
SseBI	AGGCCT	1 cut(s)	1200
SsiI	CCGC	6 cut(s)	238, 587, 2304, 2791, 2835, 2951
SspI	AATATT	2 cut(s)	2239, 2854
SspMI	CTAG	5 cut(s)	1226, 2022, 2135, 2675, 2765
SstI	GAGCTC	1 cut(s)	1121
StuI	AGGCCT	1 cut(s)	1200
StyD4I	CCNGG	6 cut(s)	342, 523, 983, 1799, 2167, 2919
StyI	CCWWGG	5 cut(s)	217, 1968, 2008, 2514, 2524
TaaI	ACNGT	7 cut(s)	807, 1492, 1960, 2084, 2424, 2596, 2659
TaiI	ACGT	1 cut(s)	834
TaqI	TCGA	8 cut(s)	254, 267, 423, 798, 1934, 2164, 2325, 2332
TatI	WGTACW	2 cut(s)	153, 1499
TauI	GCSGC	2 cut(s)	589, 2794
Tru1I	TTAA	6 cut(s)	498, 906, 1830, 2414, 2661, 3278
Tru9I	TTAA	6 cut(s)	498, 906, 1830, 2414, 2661, 3278
TscAI	CASTG	9 cut(s)	300, 1102, 1395, 1901, 2089, 2347, 2429, 2464, 2950
TseFI	GTSAC	7 cut(s)	380, 1075, 1351, 1396, 2455, 2941, 3264
Tsp45I	GTSAC	7 cut(s)	380, 1075, 1351, 1396, 2455, 2941, 3264
TspGWI	ACGGA	3 cut(s)	213, 2366, 2801
TspRI	CASTG	9 cut(s)	300, 1102, 1395, 1901, 2089, 2347, 2429, 2464, 2950
Van91I	CCANNNNNTGG	1 cut(s)	2014
VneI	GTGCAC	1 cut(s)	1892
VpaK11BI	GGWCC	3 cut(s)	2391, 2832, 3238
XagI	CCTNNNNNAGG	2 cut(s)	594, 1701
XapI	RAATTY	7 cut(s)	16, 921, 2058, 2190, 2327, 2636, 3128
XceI	RCATGY	2 cut(s)	1786, 1932
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1661
XhoI	CTCGAG	2 cut(s)	266, 1933
XmiI	GTMKAC	2 cut(s)	1977, 2164
XmnI	GAANNNNTTC	1 cut(s)	3038
XspI	CTAG	5 cut(s)	1226, 2022, 2135, 2675, 2765
Zsp2I	ATGCAT	1 cut(s)	2039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.