Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0016210

Physical Location & Seq

Genomic Coordinates Reverse (-)

39551020 .. 39554228

Gene Structure 3209 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1278400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2871 bp

ATGGCTACTTCAACCCGACACTACTGCTCCTCCACTTCCTCCGCCGTGCACCGCCACTCTATGTTGAAGAAAGCTCTGCCAAACCCGATTCTCCACTTCTTCAAACACCCTCAAAATCCCATTAAATTACCTTCCTTGAAGGAGGTTTGCGACGGAGGAAGCCTCGGGGAAGCTTTTCACTCACTCGGCAGTTTGCTCACTCATCCAAATTCCCTTCAGCTCTCTCTAGATGCGGCTTACTCGCCGGTCCTCGAGCTCTGCGCAAACGAGAAGGCTCTCTCAGAAGGGCAGCAAATCCACGCCCAGTTGATCAAATGCTGTGATGTGCCCGATTCTGCGTTTCTGAGTACTAAGCTTGTTTTTATGTACGGGAAATGTGGCTCTCTTTTGGACGCGGAGAAGGTGTTTGATAAAATGTCCCACAGAACGGTTTTCAGTTGGAACGCGATGATTGCTGCTTGTGCTTCAAACGGGAAGCCTTGGAAAGCTCTGGAGCTGTATAGGGACATGAGGGTTTTGGAGGAACCTCTCGATTCTTGCACTTTTCCTTGTATTCTGAAAGCGTGCGTTGCGATTAACAATCTCCGTTATGGGGCAGAAATTCATGGTCTGGCTATCAAATACGGGTATAGTAATGTAACACTCGTTGTCAACTCACTAGCTTCGATGTATGCAAACTGCAAATGTGTTGACGGGGCGAGGAAGTTATTTGATGGTGTGAAGGAAAAAGAGGATATTGTGTCGTGGAATTCAATTATTTCAGCGTATTCCGCAAATGGGAAATCAGCTGAAGCATTGGGGCTGTTTCGAGAAATGCTAAGCTCGGGACTTGCCCTGAACTCATATACCCTTGTCGCCGCTCTTCAAGCCTGCGAGGATTCTTTTTGTGGTAAACTTGGTATGGAGATCCATGCTGCTGTGTTGAAATCGGGTCACTTCTTTGACATTTATGTGGCGAATTCTTTGCTTGCTATGTACGTGAGATGTGGTAACATGCATGAGGCTGCAATAATCTTCAGAGACTTGGATGCCAAGGATGTTGTTTCGTGGAATACCATGCTCTCTGGTTTTTCCCAGAATGGTCTATATGACGAAACCCTGCTGTTGTTCCGAGATATGCAGAGTACTGATACAAAACCTGACCAGGTTTCACTATTAAATATCCTTGCAGCATCCGGTCGATTAGGAGATTTGTTGTCTGGGTTGGAAGCTCATGCTTATGCGATTAAAAAGGGATTCGACTCTGATTTGCAGCTCGGAAATACACTGATAGATATGTATGCTAGGTGTGGTTGTGTGAATTTCATGGGCCGTGCTTTTGACAAGATGCCTACTAAGGACTTAATTTCTTGGACAACTGTTATTGCTGGCTATGCTCAGAATAATTGTCACTTAAGGGCCTTAGAATTGTGCCGGAAGGTGCAGACGCTCCAATTGGATGCTGATGCAATGATGGTAGAAAGCATTTTAGCTGTTTGTGGGGCCTTGAAATGTGTTTCTTGGGTAAAAGAAGTTCATGGTTACGCTGTGAGAACAGGTTTATTCAATCTGGTGTTACAAAATGCAGTTGTCAACCTATATGGAGAGTGTGGATATGTGAAATATGCAGAGCTGATGTTCGAATTGATTGACTCTAAGGATATTGTGTCTTGGACGAGCATGATCTCTTGTTATGTTCATAATGGGTTTGCAAATGAGGCCCTTGAACTATGCCACTTCATGAAGGAAACAAATGTTGAACCTGATGCTATAGCACTAGTTAGTATACTATCGGCTGTTGCGAGTTTATCTGCTCTGAAGAAAGGGAAAGAGATTCATGGTTTTCTTATCCGGAAGGGCTTCATCTTAGAGGGATCTCTTGGAAGCTCTCTAGTCGATATGTATGCCCGCTCTGGAACTTTGGAGAATGCATATGAGGTATATAACTGTATTAAAAATAGAAGTCTAATTTTGTGGACTACCATGATTAATGCGTATGGAATGCATGGCCAAGGCAAGGCAGCCATTGATTTATTCGAGCAGATGGAAGGTGAAAGAAATGTTCCTGATCATATTACGTTTTTGGCTCTTCTATATGCCTGCAGTCATTCGGGCTTGATTGATGAGGGTAAAAGAATATTTGAAATTATGAGAGATGAATATCATTTGGAACCATGGCCTGAACATTCTGCCTGTATGGTTGATCTTCTTGGTCGCGCCAATCGGTTGGAAGAGGCATACCATTTTACGAATAGCATGCAGAGTGAACCTACAGCTGAGGTATGGTGTGCACTCCTTGGGGCTTGCCGGGTTCATTCTAACAAAGAATTCGGAGAAATTGCAGCAAAGAAAATCTTAGAGTTGGACCCGGAGAATCCTGGAAATTATGTGCTTGTATCTAATGTGTTTGCTGCTAGTGGAAGATGGAAAGATGTAGATGAAGTGAGGTTGAGAATGAAGGGGAGTGGATTGAAGAAAAATCCGGGATGTAGTTGGATTGAGGTTGGAAACAAGGTTCATACATTCACGGCGAGGGATAAATCTCATCCGCAGTCCGATGATATTTACCTCAAGTTAGCTCAAATCACAGAGAAATTGGAGAGAGAAGTAGATTATGTGGCTCAAACCAAATTTGTTTTGCACAATGTAGAGGAAGAAGAGAGAGTTAAAATGCTTTATGGACATAGTGAAAGGCTGGCAATTGCATACGGTCTGCTTAAAACTCCCAAAGGAACCCCTATCCGAATCACCAAGAACCTTCGGGTTTGTGGCGATTGCCATAATTTCATTAAACTAGTTTCAAAGGTTTTTAGGCGGGTGCTTGTTGTGAGGGATGCAAATAGATTTCATCACTTTGAAGATGGTATCTGTTCTTGTGGCGATTTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

957

Amino Acids

106.3

Weight (kDa)

6.38

Isoelectric Point (pI)

34.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	143 - 190	2e-07	PPR repeat family
PPR	PF01535	145 - 171	7.2e-07	PPR repeat
PPR_2	PF13041	245 - 291	4.3e-10	PPR repeat family
PPR	PF01535	247 - 277	3e-08	PPR repeat
PPR_2	PF13041	345 - 384	6.4e-08	PPR repeat family
PPR	PF01535	348 - 375	3.8e-06	PPR repeat
PPR_2	PF13041	546 - 592	2.7e-07	PPR repeat family
PPR	PF01535	549 - 576	4.1e-06	PPR repeat
PPR_2	PF13041	650 - 695	2.1e-08	PPR repeat family
PPR	PF01535	651 - 677	6.6e-06	PPR repeat
E_motif	PF20431	765 - 827	1.6e-28	E motif
Eplus_motif	PF20430	832 - 859	5.6e-09	E+ motif
DYW_deaminase	PF14432	865 - 956	1.2e-44	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016210)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63370 AT3G63370
fragaria_vesca	FvH4_2g22910
malus_domestica	MD11G1278400.v1.1
prunus_persica	Prupe.8G234400_v2.0.a1
pyrus_communis	pycom11g24590
rosa_chinensis	RchiOBHm_Chr6g0289871
rosa_laevigata	RLG00000012263
rosa_multiflora	Rmu_ssc0000314.1_g000006
rosa_rugosa	Rorug06G0207400
rosa_samantha	Rh6AG319000 Rh6BG326000 Rh6CG331900 Rh6DG317400
rosa_wichuraiana	Rw6G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	262
AccBSI	CCGCTC	2 cut(s)	860, 1890
AccI	GTMKAC	1 cut(s)	1765
AccII	CGCG	3 cut(s)	395, 446, 2196
AccIII	TCCGGA	1 cut(s)	1830
AciI	CCGC	9 cut(s)	42, 52, 233, 395, 771, 858, 1888, 2528, 2794
AclWI	GGATC	2 cut(s)	901, 1861
AcoI	YGGCCR	1 cut(s)	1987
AcsI	RAATTY	7 cut(s)	208, 600, 748, 958, 1300, 2306, 2609
AcuI	CTGAAG	4 cut(s)	200, 810, 1000, 1817
AfaI	GTAC	4 cut(s)	349, 368, 977, 1126
AfiI	CCNNNNNNNGG	4 cut(s)	427, 592, 777, 1996
AflII	CTTAAG	1 cut(s)	1393
AhlI	ACTAGT	2 cut(s)	1756, 2773
AjnI	CCWGG	2 cut(s)	1143, 2356
AloI	GAACNNNNNNTCC	2 cut(s)	2478, 2510
Alw21I	GWGCWC	3 cut(s)	51, 258, 2272
Alw26I	GTCTC	1 cut(s)	1014
Alw44I	GTGCAC	2 cut(s)	47, 2268
AlwI	GGATC	2 cut(s)	901, 1861
Ama87I	CYCGRG	3 cut(s)	164, 251, 823
Aor13HI	TCCGGA	1 cut(s)	1830
AoxI	GGCC	6 cut(s)	1309, 1398, 1482, 1698, 1987, 2156
ApaLI	GTGCAC	2 cut(s)	47, 2268
ApeKI	GCWGC	9 cut(s)	289, 455, 914, 1004, 1169, 1252, 2000, 2321, 2390
ApoI	RAATTY	7 cut(s)	208, 600, 748, 958, 1300, 2306, 2609
AseI	ATTAAT	1 cut(s)	1968
Asp700I	GAANNNNTTC	2 cut(s)	174, 1838
AspLEI	GCGC	2 cut(s)	263, 2198
AspS9I	GGNCC	6 cut(s)	247, 1309, 1398, 1482, 1699, 2344
AsuC2I	CCSGG	3 cut(s)	2288, 2348, 2463
AsuHPI	GGTGA	2 cut(s)	2042, 2719
AsuII	TTCGAA	1 cut(s)	1620
AvaI	CYCGRG	3 cut(s)	164, 251, 823
AvaII	GGWCC	2 cut(s)	247, 2344
BaeGI	GKGCMC	3 cut(s)	51, 330, 2272
BalI	TGGCCA	1 cut(s)	1989
BanII	GRGCYC	1 cut(s)	258
Bbv12I	GWGCWC	3 cut(s)	51, 258, 2272
BbvCI	CCTCAGC	1 cut(s)	2256
BbvI	GCAGC	9 cut(s)	301, 442, 901, 991, 1181, 1264, 2012, 2333, 2377
BccI	CCATC	5 cut(s)	707, 1447, 2017, 2397, 2834
BceAI	ACGGC	3 cut(s)	29, 1296, 2523
BcgI	CGANNNNNNTGC	8 cut(s)	6, 40, 946, 980, 1865, 1899, 2218, 2252
BciT130I	CCWGG	2 cut(s)	1145, 2358
BclI	TGATCA	2 cut(s)	309, 2047
BcnI	CCSGG	3 cut(s)	2288, 2348, 2463
BcoDI	GTCTC	1 cut(s)	1014
BcuI	ACTAGT	2 cut(s)	1756, 2773
BfaI	CTAG	7 cut(s)	227, 659, 1284, 1757, 1871, 2394, 2774
BfmI	CTRYAG	3 cut(s)	1749, 2080, 2250
BfrI	CTTAAG	1 cut(s)	1393
BlpI	GCTNAGC	1 cut(s)	818
BmcAI	AGTACT	2 cut(s)	349, 1126
Bme1390I	CCNGG	5 cut(s)	1145, 2288, 2348, 2358, 2463
Bme18I	GGWCC	2 cut(s)	247, 2344
BmeT110I	CYCGRG	3 cut(s)	164, 251, 823
BmgT120I	GGNCC	6 cut(s)	247, 1309, 1398, 1482, 1699, 2344
BmiI	GGNNCC	5 cut(s)	525, 1483, 2151, 2346, 2713
BmrFI	CCNGG	5 cut(s)	1145, 2288, 2348, 2358, 2463
BmrI	ACTGGG	1 cut(s)	298
BmsI	GCATC	8 cut(s)	220, 1018, 1181, 1317, 1429, 1435, 1735, 2803
BmuI	ACTGGG	1 cut(s)	298
BplI	GAGNNNNNCTC	2 cut(s)	147, 179
BpmI	CTGGAG	1 cut(s)	512
Bpu10I	CCTNAGC	1 cut(s)	2256
Bpu1102I	GCTNAGC	1 cut(s)	818
Bpu14I	TTCGAA	1 cut(s)	1620
BpuEI	CTTGAG	1 cut(s)	2534
BpuMI	CCSGG	3 cut(s)	2288, 2348, 2463
BsaAI	YACGTR	1 cut(s)	979
BsaBI	GATNNNNATC	1 cut(s)	2139
BsaJI	CCNNGG	6 cut(s)	163, 479, 1032, 1990, 2153, 2275
BsaWI	WCCGGW	2 cut(s)	1175, 1830
BsaXI	ACNNNNNCTCC	6 cut(s)	11, 14, 41, 44, 389, 419
Bsc4I	CCNNNNNNNGG	4 cut(s)	427, 592, 777, 1996
Bse118I	RCCGGY	1 cut(s)	244
Bse1I	ACTGG	1 cut(s)	304
Bse3DI	GCAATG	1 cut(s)	1455
Bse8I	GATNNNNATC	1 cut(s)	2139
BseAI	TCCGGA	1 cut(s)	1830
BseBI	CCWGG	2 cut(s)	1145, 2358
BseDI	CCNNGG	6 cut(s)	163, 479, 1032, 1990, 2153, 2275
BseGI	GGATG	8 cut(s)	202, 1033, 1042, 1172, 1444, 2471, 2524, 2818
BseJI	GATNNNNATC	1 cut(s)	2139
BseLI	CCNNNNNNNGG	4 cut(s)	427, 592, 777, 1996
BseMI	GCAATG	1 cut(s)	1455
BseMII	CTCAG	4 cut(s)	294, 335, 1391, 2247
BseNI	ACTGG	1 cut(s)	304
BseRI	GAGGAG	1 cut(s)	19
BseSI	GKGCMC	3 cut(s)	51, 330, 2272
BseXI	GCAGC	9 cut(s)	301, 442, 901, 991, 1181, 1264, 2012, 2333, 2377
BsgI	GTGCAG	1 cut(s)	1442
Bsh1236I	CGCG	3 cut(s)	395, 446, 2196
Bsh1285I	CGRYCG	1 cut(s)	1180
BshFI	GGCC	6 cut(s)	1311, 1400, 1484, 1700, 1989, 2158
BsiEI	CGRYCG	1 cut(s)	1180
BsiHKAI	GWGCWC	3 cut(s)	51, 258, 2272
BsiHKCI	CYCGRG	3 cut(s)	164, 251, 823
BsiSI	CCGG	7 cut(s)	245, 1176, 1414, 1831, 2287, 2348, 2462
BslFI	GGGAC	3 cut(s)	403, 518, 840
BslI	CCNNNNNNNGG	4 cut(s)	427, 592, 777, 1996
BsmAI	GTCTC	1 cut(s)	1014
BsmFI	GGGAC	3 cut(s)	403, 518, 840
BsmI	GAATGC	2 cut(s)	1912, 1986
BsnI	GGCC	6 cut(s)	1311, 1400, 1484, 1700, 1989, 2158
BsoBI	CYCGRG	3 cut(s)	164, 251, 823
Bsp119I	TTCGAA	1 cut(s)	1620
Bsp1286I	GDGCHC	4 cut(s)	51, 258, 330, 2272
Bsp13I	TCCGGA	1 cut(s)	1830
Bsp143I	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
Bsp1720I	GCTNAGC	1 cut(s)	818
Bsp19I	CCATGG	1 cut(s)	2153
BspACI	CCGC	9 cut(s)	42, 52, 233, 395, 771, 858, 1888, 2528, 2794
BspANI	GGCC	6 cut(s)	1311, 1400, 1484, 1700, 1989, 2158
BspCNI	CTCAG	4 cut(s)	293, 336, 1390, 2248
BspEI	TCCGGA	1 cut(s)	1830
BspFNI	CGCG	3 cut(s)	395, 446, 2196
BspHI	TCATGA	1 cut(s)	1719
BspLI	GGNNCC	5 cut(s)	525, 1483, 2151, 2346, 2713
BspMAI	CTGCAG	1 cut(s)	2084
BspPI	GGATC	2 cut(s)	901, 1861
BspQI	GCTCTTC	2 cut(s)	867, 2073
BspT104I	TTCGAA	1 cut(s)	1620
BspTI	CTTAAG	1 cut(s)	1393
BsrBI	CCGCTC	2 cut(s)	860, 1890
BsrDI	GCAATG	1 cut(s)	1455
BsrFI	RCCGGY	1 cut(s)	244
BsrI	ACTGG	1 cut(s)	304
BssAI	RCCGGY	1 cut(s)	244
BssECI	CCNNGG	6 cut(s)	163, 479, 1032, 1990, 2153, 2275
BssMI	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
BssNAI	GTATAC	1 cut(s)	1766
BssT1I	CCWWGG	5 cut(s)	479, 1032, 1990, 2153, 2275
Bst1107I	GTATAC	1 cut(s)	1766
Bst2UI	CCWGG	2 cut(s)	1145, 2358
Bst4CI	ACNGT	4 cut(s)	430, 1360, 1928, 2690
Bst6I	CTCTTC	4 cut(s)	867, 2073, 2205, 2631
BstAFI	CTTAAG	1 cut(s)	1393
BstBAI	YACGTR	1 cut(s)	979
BstBI	TTCGAA	1 cut(s)	1620
BstC8I	GCNNGC	9 cut(s)	565, 871, 969, 1369, 1888, 2080, 2237, 2284, 2676
BstDSI	CCRYGG	1 cut(s)	2153
BstF5I	GGATG	8 cut(s)	202, 1033, 1042, 1172, 1444, 2471, 2524, 2818
BstFNI	CGCG	3 cut(s)	395, 446, 2196
BstHHI	GCGC	2 cut(s)	263, 2198
BstKTI	GATC	6 cut(s)	312, 909, 1665, 1856, 2050, 2185
BstMAI	GTCTC	1 cut(s)	1014
BstMBI	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
BstMCI	CGRYCG	1 cut(s)	1180
BstMWI	GCNNNNNNNGC	7 cut(s)	452, 461, 569, 770, 866, 1373, 1697
BstNI	CCWGG	2 cut(s)	1145, 2358
BstNSI	RCATGY	2 cut(s)	997, 2239
BstSCI	CCNGG	5 cut(s)	1143, 2286, 2346, 2356, 2461
BstSFI	CTRYAG	3 cut(s)	1749, 2080, 2250
BstSLI	GKGCMC	3 cut(s)	51, 330, 2272
BstUI	CGCG	3 cut(s)	395, 446, 2196
BstV1I	GCAGC	9 cut(s)	301, 442, 901, 991, 1181, 1264, 2012, 2333, 2377
BstX2I	RGATCY	2 cut(s)	906, 1853
BstXI	CCANNNNNNTGG	1 cut(s)	2206
BstYI	RGATCY	2 cut(s)	906, 1853
BstZ17I	GTATAC	1 cut(s)	1766
BsuRI	GGCC	6 cut(s)	1311, 1400, 1484, 1700, 1989, 2158
BtgI	CCRYGG	1 cut(s)	2153
BtgZI	GCGATG	1 cut(s)	461
BtsCI	GGATG	8 cut(s)	202, 1033, 1042, 1172, 1444, 2471, 2524, 2818
BtsIMutI	CAGTG	1 cut(s)	1265
Cac8I	GCNNGC	9 cut(s)	565, 871, 969, 1369, 1888, 2080, 2237, 2284, 2676
CciI	TCATGA	1 cut(s)	1719
CfoI	GCGC	2 cut(s)	263, 2198
Cfr10I	RCCGGY	1 cut(s)	244
Cfr13I	GGNCC	6 cut(s)	247, 1309, 1398, 1482, 1699, 2344
CseI	GACGC	2 cut(s)	401, 1435
CsiI	ACCWGGT	1 cut(s)	1143
Csp6I	GTAC	4 cut(s)	348, 367, 976, 1125
CviQI	GTAC	4 cut(s)	348, 367, 976, 1125
DpnI	GATC	6 cut(s)	311, 908, 1664, 1855, 2049, 2184
DpnII	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
EaeI	YGGCCR	1 cut(s)	1987
Eam1104I	CTCTTC	4 cut(s)	867, 2073, 2205, 2631
EarI	CTCTTC	4 cut(s)	867, 2073, 2205, 2631
EciI	GGCGGA	1 cut(s)	31
Ecl136II	GAGCTC	1 cut(s)	256
Eco130I	CCWWGG	5 cut(s)	479, 1032, 1990, 2153, 2275
Eco24I	GRGCYC	1 cut(s)	258
Eco47I	GGWCC	2 cut(s)	247, 2344
Eco53kI	GAGCTC	1 cut(s)	256
Eco57I	CTGAAG	4 cut(s)	200, 810, 1000, 1817
Eco88I	CYCGRG	3 cut(s)	164, 251, 823
EcoICRI	GAGCTC	1 cut(s)	256
EcoO109I	RGGNCCY	3 cut(s)	1398, 1482, 1699
EcoRI	GAATTC	3 cut(s)	748, 958, 2306
EcoRII	CCWGG	2 cut(s)	1143, 2356
EcoT14I	CCWWGG	5 cut(s)	479, 1032, 1990, 2153, 2275
EcoT22I	ATGCAT	3 cut(s)	999, 1912, 1986
EcoT38I	GRGCYC	1 cut(s)	258
ErhI	CCWWGG	5 cut(s)	479, 1032, 1990, 2153, 2275
FalI	AAGNNNNNCTT	2 cut(s)	2318, 2350
FaqI	GGGAC	3 cut(s)	403, 518, 840
FauI	CCCGC	2 cut(s)	1895, 2787
FauNDI	CATATG	1 cut(s)	1912
FbaI	TGATCA	2 cut(s)	309, 2047
FblI	GTMKAC	1 cut(s)	1765
FokI	GGATG	8 cut(s)	189, 1040, 1049, 1159, 1451, 2478, 2511, 2825
FriOI	GRGCYC	1 cut(s)	258
FspBI	CTAG	7 cut(s)	227, 659, 1284, 1757, 1871, 2394, 2774
FspI	TGCGCA	1 cut(s)	262
GlaI	GCGC	2 cut(s)	262, 2197
GsuI	CTGGAG	1 cut(s)	512
HaeIII	GGCC	6 cut(s)	1311, 1400, 1484, 1700, 1989, 2158
HapII	CCGG	7 cut(s)	245, 1176, 1414, 1831, 2287, 2348, 2462
HgaI	GACGC	2 cut(s)	401, 1435
HhaI	GCGC	2 cut(s)	263, 2198
Hin6I	GCGC	2 cut(s)	261, 2196
HinP1I	GCGC	2 cut(s)	261, 2196
HincII	GTYRAC	3 cut(s)	652, 691, 1573
HindII	GTYRAC	3 cut(s)	652, 691, 1573
HindIII	AAGCTT	2 cut(s)	171, 353
HpaII	CCGG	7 cut(s)	245, 1176, 1414, 1831, 2287, 2348, 2462
HphI	GGTGA	2 cut(s)	2042, 2719
Hpy166II	GTNNAC	9 cut(s)	49, 652, 691, 893, 1573, 1766, 1956, 2246, 2270
Hpy188III	TCNNGA	9 cut(s)	227, 491, 530, 809, 825, 1720, 1831, 1893, 2045
Hpy8I	GTNNAC	9 cut(s)	49, 652, 691, 893, 1573, 1766, 1956, 2246, 2270
Hpy99I	CGWCG	1 cut(s)	155
HpyCH4III	ACNGT	4 cut(s)	430, 1360, 1928, 2690
HpyCH4IV	ACGT	2 cut(s)	978, 2057
HpyF10VI	GCNNNNNNNGC	7 cut(s)	452, 461, 569, 770, 866, 1373, 1697
HpySE526I	ACGT	2 cut(s)	978, 2057
HspAI	GCGC	2 cut(s)	261, 2196
Kpn2I	TCCGGA	1 cut(s)	1830
Ksp22I	TGATCA	2 cut(s)	309, 2047
Kzo9I	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
LguI	GCTCTTC	2 cut(s)	867, 2073
LmnI	GCTCC	3 cut(s)	32, 493, 1434
Lsp1109I	GCAGC	9 cut(s)	301, 442, 901, 991, 1181, 1264, 2012, 2333, 2377
LweI	GCATC	8 cut(s)	220, 1018, 1181, 1317, 1429, 1435, 1735, 2803
MabI	ACCWGGT	1 cut(s)	1143
MaeI	CTAG	7 cut(s)	227, 659, 1284, 1757, 1871, 2394, 2774
MaeII	ACGT	2 cut(s)	978, 2057
MaeIII	GTNAC	6 cut(s)	637, 932, 989, 1388, 1520, 1554
MalI	GATC	6 cut(s)	311, 908, 1664, 1855, 2049, 2184
MbiI	CCGCTC	2 cut(s)	860, 1890
MboI	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
MfeI	CAATTG	2 cut(s)	1433, 2679
MflI	RGATCY	2 cut(s)	906, 1853
MhlI	GDGCHC	4 cut(s)	51, 258, 330, 2272
MlsI	TGGCCA	1 cut(s)	1989
MluNI	TGGCCA	1 cut(s)	1989
MlyI	GAGTC	2 cut(s)	1235, 1625
MmeI	TCCRAC	6 cut(s)	419, 1185, 2187, 2322, 2453, 2464
Mox20I	TGGCCA	1 cut(s)	1989
Mph1103I	ATGCAT	3 cut(s)	999, 1912, 1986
MroI	TCCGGA	1 cut(s)	1830
MroXI	GAANNNNTTC	2 cut(s)	174, 1838
MscI	TGGCCA	1 cut(s)	1989
MslI	CAYNNNNRTG	2 cut(s)	950, 1218
Msp20I	TGGCCA	1 cut(s)	1989
MspA1I	CMGCKG	2 cut(s)	788, 2255
MspCI	CTTAAG	1 cut(s)	1393
MspI	CCGG	7 cut(s)	245, 1176, 1414, 1831, 2287, 2348, 2462
MspR9I	CCNGG	5 cut(s)	1145, 2288, 2348, 2358, 2463
MunI	CAATTG	2 cut(s)	1433, 2679
Mva1269I	GAATGC	2 cut(s)	1912, 1986
MvaI	CCWGG	2 cut(s)	1145, 2358
MvnI	CGCG	3 cut(s)	395, 446, 2196
MwoI	GCNNNNNNNGC	7 cut(s)	452, 461, 569, 770, 866, 1373, 1697
NciI	CCSGG	3 cut(s)	2288, 2348, 2463
NcoI	CCATGG	1 cut(s)	2153
NdeI	CATATG	1 cut(s)	1912
NdeII	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
NlaIV	GGNNCC	5 cut(s)	525, 1483, 2151, 2346, 2713
NmeAIII	GCCGAG	1 cut(s)	165
NmuCI	GTSAC	2 cut(s)	932, 1388
NsbI	TGCGCA	1 cut(s)	262
NsiI	ATGCAT	3 cut(s)	999, 1912, 1986
NspI	RCATGY	2 cut(s)	997, 2239
NspV	TTCGAA	1 cut(s)	1620
PaeI	GCATGC	1 cut(s)	2239
PaeR7I	CTCGAG	1 cut(s)	251
PagI	TCATGA	1 cut(s)	1719
PciSI	GCTCTTC	2 cut(s)	867, 2073
PcsI	WCGNNNNNNNCGW	1 cut(s)	1778
PctI	GAATGC	2 cut(s)	1912, 1986
PdmI	GAANNNNTTC	2 cut(s)	174, 1838
PfeI	GAWTC	8 cut(s)	88, 332, 533, 878, 1236, 1813, 2353, 2724
PfoI	TCCNGGA	2 cut(s)	2356, 2461
PleI	GAGTC	2 cut(s)	1235, 1625
PpsI	GAGTC	2 cut(s)	1235, 1625
Ppu21I	YACGTR	1 cut(s)	979
PshBI	ATTAAT	1 cut(s)	1968
Psp124BI	GAGCTC	1 cut(s)	258
Psp6I	CCWGG	2 cut(s)	1143, 2356
PspGI	CCWGG	2 cut(s)	1143, 2356
PspN4I	GGNNCC	5 cut(s)	525, 1483, 2151, 2346, 2713
PspPI	GGNCC	6 cut(s)	247, 1309, 1398, 1482, 1699, 2344
PspXI	VCTCGAGB	1 cut(s)	251
PsrI	GAACNNNNNNTAC	2 cut(s)	1497, 1529
PstI	CTGCAG	1 cut(s)	2084
PsuI	RGATCY	2 cut(s)	906, 1853
PvuII	CAGCTG	2 cut(s)	788, 2255
RsaI	GTAC	4 cut(s)	349, 368, 977, 1126
RsaNI	GTAC	4 cut(s)	348, 367, 976, 1125
RseI	CAYNNNNRTG	2 cut(s)	950, 1218
SacI	GAGCTC	1 cut(s)	258
SapI	GCTCTTC	2 cut(s)	867, 2073
Sau3AI	GATC	6 cut(s)	309, 906, 1662, 1853, 2047, 2182
Sau96I	GGNCC	6 cut(s)	247, 1309, 1398, 1482, 1699, 2344
ScaI	AGTACT	2 cut(s)	349, 1126
SchI	GAGTC	2 cut(s)	1235, 1625
ScrFI	CCNGG	5 cut(s)	1145, 2288, 2348, 2358, 2463
SduI	GDGCHC	4 cut(s)	51, 258, 330, 2272
SexAI	ACCWGGT	1 cut(s)	1143
SfaNI	GCATC	8 cut(s)	220, 1018, 1181, 1317, 1429, 1435, 1735, 2803
SfcI	CTRYAG	3 cut(s)	1749, 2080, 2250
Sfr274I	CTCGAG	1 cut(s)	251
SfuI	TTCGAA	1 cut(s)	1620
SinI	GGWCC	2 cut(s)	247, 2344
SlaI	CTCGAG	1 cut(s)	251
SmiMI	CAYNNNNRTG	2 cut(s)	950, 1218
SmlI	CTYRAG	3 cut(s)	251, 1393, 2549
SmoI	CTYRAG	3 cut(s)	251, 1393, 2549
SpeI	ACTAGT	2 cut(s)	1756, 2773
SphI	GCATGC	1 cut(s)	2239
SsiI	CCGC	9 cut(s)	42, 52, 233, 395, 771, 858, 1888, 2528, 2794
SspI	AATATT	1 cut(s)	2118
SspMI	CTAG	7 cut(s)	227, 659, 1284, 1757, 1871, 2394, 2774
SstI	GAGCTC	1 cut(s)	258
StyD4I	CCNGG	5 cut(s)	1143, 2286, 2346, 2356, 2461
StyI	CCWWGG	5 cut(s)	479, 1032, 1990, 2153, 2275
TaaI	ACNGT	4 cut(s)	430, 1360, 1928, 2690
TaiI	ACGT	2 cut(s)	981, 2060
TaqI	TCGA	9 cut(s)	252, 531, 665, 808, 1180, 1239, 1620, 1875, 2016
TatI	WGTACW	2 cut(s)	347, 1124
TauI	GCSGC	2 cut(s)	236, 860
TfiI	GAWTC	8 cut(s)	88, 332, 533, 878, 1236, 1813, 2353, 2724
TscAI	CASTG	1 cut(s)	1272
TseFI	GTSAC	2 cut(s)	932, 1388
TseI	GCWGC	9 cut(s)	289, 455, 914, 1004, 1169, 1252, 2000, 2321, 2390
Tsp45I	GTSAC	2 cut(s)	932, 1388
TspGWI	ACGGA	2 cut(s)	168, 575
TspRI	CASTG	1 cut(s)	1272
Vha464I	CTTAAG	1 cut(s)	1393
VneI	GTGCAC	2 cut(s)	47, 2268
VpaK11BI	GGWCC	2 cut(s)	247, 2344
VspI	ATTAAT	1 cut(s)	1968
XapI	RAATTY	7 cut(s)	208, 600, 748, 958, 1300, 2306, 2609
XbaI	TCTAGA	1 cut(s)	226
XceI	RCATGY	2 cut(s)	997, 2239
XhoI	CTCGAG	1 cut(s)	251
XmiI	GTMKAC	1 cut(s)	1765
XmnI	GAANNNNTTC	2 cut(s)	174, 1838
XspI	CTAG	7 cut(s)	227, 659, 1284, 1757, 1871, 2394, 2774
ZrmI	AGTACT	2 cut(s)	349, 1126
Zsp2I	ATGCAT	3 cut(s)	999, 1912, 1986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.