Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0016210

Physical Location & Seq

Genomic Coordinates Reverse (-)

51350278 .. 51353649

Gene Structure 3372 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G027510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2871 bp

ATGGCTACTTCGTCTCCACACAGTTACACCACTCTTTCTGCCGTCCAATTCCGACCCATGTTGAACAAAGCCCAGCTAAACCCAGCTCTCCATTTCTTCAAATTCTCCGAAAAGCCCACCAAATTATTACCTTCCTTGAAGCAGGTTTGCGATGAAGGCAGCCTCAGGGAAGCTTTTACATCACTCAGCAATTTGCTAACCCATCAAGATTCACTCGAGCTTTCTTTAGATGGAGCTTACTCGCCGCTCCTTGAGCTCTGTGCAAAGAGGAAGGCTCTATCAGAAGGGCAGCAAATCCATGCCCACTTGATTAAATCCGGTGCTGTATGGGATTCCGCGTTTCTGAGTACCAAGCTTGTTTATATGTACGGGAAATGTGGCTCCGTTATGAATGCACAGAAGGTGTTTGATAAAATGTCTCACAGAACGATTTTCACTTGGAACGCAATGATTGGTGCTTGTGCGTCAAACGGGGAGCCTCTAAAGGCTCTTGAGATGTACTGTGACATGCGGGTTCTCGGGGTTCCTCTTGATTCGTTTACTTTCCCTTGCGTACTGAAAGCATGTGTTGCAGTTAATAGTCTCTGTTGTGGGGAAGAAATTCATGGCCTGGCTATCAAATGTGGGTATGATGATGTAGCATTTGTGGTTAACTCGCTAGTTGCAATGTATGCAAACTGCAATGATCTTGGTGCAGCGAGGAAATTGTTTGATGGTATGAGAGAAAAGGAGGATGTTGTGTCATGGAATTCTATTATTTCAGCATATTCAGCAAGGGGGATGTCCGTTGAAGCGTTGGGATTGTTTCGAGAAATGCAGCAGGTGGGACTTGCCATGAACACGTATACTTTTGTGGCTGCCCTTCAAGCGTGTGAGGATTCTTTTTGTGGAAAACTCAGTATGGAGATTCATGCTGCTGTGTTGAAACTGAATTATTGTTTTGATATTTATGTGGCGAATTCTCTGCTTGCAATGTATGTGAGGTGTGGTAAAATGGATGAGGCTGCAAGGATTTTTAATGACTTGGATGACAAGGATATTGTCTCATGGAATACATTACTCTCTGGTTTTGTCCAAAATGGACTATATGAGGAAGCCCTGCTGTTGTTCCATGATATGCAGAGGATTGGTCATAAACCTGACCAGGTTTCGTTGTTGAATATCCTTGCAGCATCTGGTCGGTTAGGAAGTTTATTGTCAGGAATGGAAGCTCATGCTTATGTGATAAAAAATGGATTCGATTCTGATTTGCAGGTTGGGAACACACTGATAGATATGTATGCTAAGTGTTGTTGTGTGAACTTGATGGGCCGTGCTTTTGACAAGATGCCAGACAAAGACTTCATTTCTTGGACAACAATTATTGCTGGCTATGCTCAGAACAATTGTCACATAAGGGCCTTAAAACTGTGCCAGAAGGTGCAAATGTTAGGGCTGGAAGTGGATGCGATGATGGTACAAAGCATTTTACTGGCTTGCGAATCTTTGAAATGTGTTTCTTTGGTAAAAGAAGTTCATGGTTACGCTATGAGGAGAGGTTTATTTGATCTCGTCTTGCAAAATGCAGTTGTCAATGTATATGGCCAGTGTGGTTACATAGATTACGCATATCGGATGTTCAAGTTGATTGAATCTAAAGATGTTGTGTCTTGGACGAGTATGATATCTTGTTATGTTCATAATGGGCTTGCAAATGAGGCTCTTGAACTTTGTCACTCCATGAAAGAAACAAATGTTGAACCTGATGCCATAGCACTTGTAAGTATACTATCTGCTGCCGCTAGTTTGTCTGCTTTGATGAAAGGGAAAGAAATTCACGGTTTTCTTACTAGGAAGGGCTTCATCTTGGAGGGATCTGTTGCGAGCTCTCTTGTTGATATGTATGCCCGCTGTGGAAATCTGAAGAATGCATACAACATATATAACTGTGTTAGAAACAAAAGTCTAATTTTATGGACTACCATGATCAATGCTTATGGGATGCATGGTCATGGCAAGGAAGCCATTGGTTTCTTCAGAAGGATGCAAAACCAAGAGATTGTTCCTGATCATATTACGTTTTTGGCTCTTCTATATGCCTGCAGTCATTCAGGCTTGATTGATGAGGGTAAAGGACTATTTGAAATCATGATACATGAGTATCAATTAGACCCCTGGCCAGAACATTTTGCTTGCATGGTTGATCTTCTTGGACGTGCCAATCGATTGGAAGAGGCGTATCATTTTGTCAATAGCATGGAGAGTCAACCTACTGCTGAAGTATGGTGTGCTCTTCTTGGGGCTTGCCGAGTTCATTCTAACAAAGGATTAGAAGAAATTGCTGCAAAGAAGATCTTAGAGTTGGGCACCAAGAATCCAGGAAATTATGTGCTTGTGTCGAATATCTTTGCTGCTAGGGGAAGATGGGAACATGTAGAAGAAGTGAGGATCAGAATGAAGGTAATTGGGTTGAAGAAAAATCCAGGATGTAGTTGGATTGAGGCAGGAAAAAAAGTTCATACATTCACGGCTAGGGATAAGTCTCATCCTCAGTCCAATGAAATTTACCAAAAGTTAGCTCAAATAACTGAGACATTAGAGAGGGAATCTGGCTATGTGGCTCAAACTAAATGTGTGTTGCAGAATGTAGAGGAAGAAGAGAAAGTTCAGATGCTTTATGGACACAGTGAAAGGTTAGCCATTGCATATGGTCTACTTAAGATTCCTGAGGGAACTCCTATTCGAATCACAAAGAACCTGCGGGTTTGTATGGATTGCCATACTTTTACTAAACTAGTTTCCAAAGTTTTTAGGCGGGTGCTTGTAGTTAGGGATGCTAATAGATTTCATCATTTCGAGGGTGGAAAATGTTCTTGTGGCGATTTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

956

Amino Acids

106.72

Weight (kDa)

6.99

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	143 - 190	7.3e-10	PPR repeat family
PPR	PF01535	145 - 175	2.7e-07	PPR repeat
PPR_2	PF13041	245 - 291	3.8e-10	PPR repeat family
PPR	PF01535	247 - 276	2.8e-07	PPR repeat
PPR_2	PF13041	345 - 384	7.9e-10	PPR repeat family
PPR	PF01535	348 - 377	1.8e-08	PPR repeat
PPR_2	PF13041	546 - 591	7.7e-09	PPR repeat family
PPR	PF01535	549 - 576	6.9e-07	PPR repeat
PPR_2	PF13041	649 - 695	2.4e-08	PPR repeat family
PPR	PF01535	651 - 677	6.9e-06	PPR repeat
E_motif	PF20431	765 - 827	6.9e-24	E motif
Eplus_motif	PF20430	832 - 856	8.7e-11	E+ motif
DYW_deaminase	PF14432	864 - 956	1.3e-42	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016210)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63370 AT3G63370
fragaria_vesca	FvH4_2g22910
malus_domestica	MD11G1278400.v1.1
prunus_persica	Prupe.8G234400_v2.0.a1
pyrus_communis	pycom11g24590
rosa_chinensis	RchiOBHm_Chr6g0289871
rosa_laevigata	RLG00000012263
rosa_multiflora	Rmu_ssc0000314.1_g000006
rosa_rugosa	Rorug06G0207400
rosa_samantha	Rh6AG319000 Rh6BG326000 Rh6CG331900 Rh6DG317400
rosa_wichuraiana	Rw6G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	811
Acc36I	ACCTGC	4 cut(s)	133, 811, 1243, 2745
AccB1I	GGYRCC	1 cut(s)	2345
AccBSI	CCGCTC	1 cut(s)	247
AccI	GTMKAC	3 cut(s)	845, 1765, 2692
AccII	CGCG	1 cut(s)	338
AciI	CCGC	7 cut(s)	245, 336, 511, 1779, 1888, 2740, 2794
AclWI	GGATC	2 cut(s)	1861, 2435
AcoI	YGGCCR	2 cut(s)	1582, 2156
AcsI	RAATTY	6 cut(s)	101, 600, 748, 958, 1812, 2541
AcuI	CTGAAG	3 cut(s)	1922, 1999, 2277
AfaI	GTAC	5 cut(s)	349, 368, 500, 555, 1458
AflII	CTTAAG	1 cut(s)	2696
AflIII	ACRYGT	2 cut(s)	840, 2410
AhlI	ACTAGT	1 cut(s)	2773
AjiI	CACGTC	1 cut(s)	2195
AjnI	CCWGG	5 cut(s)	609, 1143, 2153, 2356, 2461
AjuI	GAANNNNNNNTTGG	2 cut(s)	2025, 2057
AloI	GAACNNNNNNTCC	2 cut(s)	2478, 2510
Alw21I	GWGCWC	3 cut(s)	258, 1868, 2272
Alw26I	GTCTC	6 cut(s)	18, 423, 587, 1048, 2526, 2564
AlwI	GGATC	2 cut(s)	1861, 2435
AlwNI	CAGNNNCTG	1 cut(s)	1175
Ama87I	CYCGRG	2 cut(s)	215, 518
AoxI	GGCC	5 cut(s)	607, 1309, 1398, 1582, 2156
ApoI	RAATTY	6 cut(s)	101, 600, 748, 958, 1812, 2541
Asp700I	GAANNNNTTC	3 cut(s)	600, 1838, 2848
AspS9I	GGNCC	2 cut(s)	1309, 1398
AsuII	TTCGAA	1 cut(s)	2722
AvaI	CYCGRG	2 cut(s)	215, 518
AxyI	CCTNAGG	2 cut(s)	164, 2706
BaeGI	GKGCMC	1 cut(s)	2348
BaeI	ACNNNNGTAYC	4 cut(s)	2123, 2123, 2156, 2156
BalI	TGGCCA	2 cut(s)	1584, 2158
BanI	GGYRCC	1 cut(s)	2345
BanII	GRGCYC	2 cut(s)	258, 1868
BarI	GAAGNNNNNNTAC	2 cut(s)	1895, 1927
Bbv12I	GWGCWC	3 cut(s)	258, 1868, 2272
BccI	CCATC	6 cut(s)	210, 224, 707, 1300, 1447, 2397
BceAI	ACGGC	3 cut(s)	26, 1296, 2523
BcgI	CGANNNNNNTGC	2 cut(s)	946, 980
BciT130I	CCWGG	5 cut(s)	611, 1145, 2155, 2358, 2463
BclI	TGATCA	2 cut(s)	1965, 2047
BcoDI	GTCTC	6 cut(s)	18, 423, 587, 1048, 2526, 2564
BcuI	ACTAGT	1 cut(s)	2773
BfaI	CTAG	6 cut(s)	659, 1782, 1830, 2394, 2511, 2774
BfmI	CTRYAG	1 cut(s)	2080
BfrI	CTTAAG	1 cut(s)	2696
BfuAI	ACCTGC	4 cut(s)	133, 811, 1243, 2745
BglII	AGATCT	1 cut(s)	2331
Bme1390I	CCNGG	5 cut(s)	611, 1145, 2155, 2358, 2463
BmeT110I	CYCGRG	2 cut(s)	215, 518
BmgBI	CACGTC	1 cut(s)	2195
BmgT120I	GGNCC	2 cut(s)	1309, 1398
BmiI	GGNNCC	4 cut(s)	382, 477, 525, 2347
BmrFI	CCNGG	5 cut(s)	611, 1145, 2155, 2358, 2463
BmsI	GCATC	8 cut(s)	1181, 1317, 1435, 1735, 1971, 2013, 2640, 2803
BplI	GAGNNNNNCTC	2 cut(s)	259, 291
Bpu14I	TTCGAA	1 cut(s)	2722
BpuEI	CTTGAG	2 cut(s)	272, 512
Bsa29I	ATCGAT	1 cut(s)	2203
BsaAI	YACGTR	1 cut(s)	843
BsaJI	CCNNGG	1 cut(s)	2153
BsaWI	WCCGGW	1 cut(s)	317
BsaXI	ACNNNNNCTCC	1 cut(s)	28
Bse1I	ACTGG	2 cut(s)	1476, 1585
Bse21I	CCTNAGG	2 cut(s)	164, 2706
Bse3DI	GCAATG	5 cut(s)	453, 672, 688, 978, 2679
BseBI	CCWGG	5 cut(s)	611, 1145, 2155, 2358, 2463
BseCI	ATCGAT	1 cut(s)	2203
BseDI	CCNNGG	1 cut(s)	2153
BseMI	GCAATG	5 cut(s)	453, 672, 688, 978, 2679
BseMII	CTCAG	8 cut(s)	178, 199, 335, 910, 1391, 2543, 2559, 2697
BseNI	ACTGG	2 cut(s)	1476, 1585
BseRI	GAGGAG	1 cut(s)	1546
BseSI	GKGCMC	1 cut(s)	2348
BseYI	CCCAGC	2 cut(s)	72, 82
BsgI	GTGCAG	1 cut(s)	714
Bsh1236I	CGCG	1 cut(s)	338
BshFI	GGCC	5 cut(s)	609, 1311, 1400, 1584, 2158
BshNI	GGYRCC	1 cut(s)	2345
BshVI	ATCGAT	1 cut(s)	2203
BsiHKAI	GWGCWC	3 cut(s)	258, 1868, 2272
BsiHKCI	CYCGRG	2 cut(s)	215, 518
BsiSI	CCGG	1 cut(s)	318
BslFI	GGGAC	1 cut(s)	840
BsmAI	GTCTC	6 cut(s)	18, 423, 587, 1048, 2526, 2564
BsmBI	CGTCTC	1 cut(s)	18
BsmFI	GGGAC	1 cut(s)	840
BsmI	GAATGC	2 cut(s)	397, 1912
BsnI	GGCC	5 cut(s)	609, 1311, 1400, 1584, 2158
BsoBI	CYCGRG	2 cut(s)	215, 518
Bsp119I	TTCGAA	1 cut(s)	2722
Bsp1286I	GDGCHC	4 cut(s)	258, 1868, 2272, 2348
Bsp143I	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
BspACI	CCGC	7 cut(s)	245, 336, 511, 1779, 1888, 2740, 2794
BspANI	GGCC	5 cut(s)	609, 1311, 1400, 1584, 2158
BspCNI	CTCAG	8 cut(s)	177, 198, 336, 909, 1390, 2542, 2560, 2698
BspDI	ATCGAT	1 cut(s)	2203
BspFNI	CGCG	1 cut(s)	338
BspHI	TCATGA	1 cut(s)	2127
BspLI	GGNNCC	4 cut(s)	382, 477, 525, 2347
BspMAI	CTGCAG	1 cut(s)	2084
BspMI	ACCTGC	4 cut(s)	133, 811, 1243, 2745
BspPI	GGATC	2 cut(s)	1861, 2435
BspQI	GCTCTTC	2 cut(s)	2073, 2277
BspT104I	TTCGAA	1 cut(s)	2722
BspT107I	GGYRCC	1 cut(s)	2345
BspTI	CTTAAG	1 cut(s)	2696
BsrBI	CCGCTC	1 cut(s)	247
BsrDI	GCAATG	5 cut(s)	453, 672, 688, 978, 2679
BsrI	ACTGG	2 cut(s)	1476, 1585
BssECI	CCNNGG	1 cut(s)	2153
BssMI	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
BssNAI	GTATAC	2 cut(s)	846, 1766
Bst1107I	GTATAC	2 cut(s)	846, 1766
Bst2UI	CCWGG	5 cut(s)	611, 1145, 2155, 2358, 2463
Bst4CI	ACNGT	6 cut(s)	23, 503, 1410, 1820, 1928, 2666
Bst6I	CTCTTC	4 cut(s)	2073, 2205, 2277, 2631
BstAFI	CTTAAG	1 cut(s)	2696
BstAPI	GCANNNNNTGC	2 cut(s)	569, 671
BstBAI	YACGTR	1 cut(s)	843
BstBI	TTCGAA	1 cut(s)	2722
BstC8I	GCNNGC	9 cut(s)	969, 1369, 1477, 1689, 1864, 1888, 2080, 2173, 2284
BstFNI	CGCG	1 cut(s)	338
BstKTI	GATC	8 cut(s)	688, 1549, 1856, 1968, 2050, 2185, 2334, 2430
BstMAI	GTCTC	6 cut(s)	18, 423, 587, 1048, 2526, 2564
BstMBI	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
BstMWI	GCNNNNNNNGC	8 cut(s)	156, 253, 569, 671, 770, 866, 1373, 1697
BstNI	CCWGG	5 cut(s)	611, 1145, 2155, 2358, 2463
BstNSI	RCATGY	3 cut(s)	511, 567, 2414
BstSCI	CCNGG	5 cut(s)	609, 1143, 2153, 2356, 2461
BstSFI	CTRYAG	1 cut(s)	2080
BstSLI	GKGCMC	1 cut(s)	2348
BstUI	CGCG	1 cut(s)	338
BstX2I	RGATCY	2 cut(s)	1853, 2331
BstXI	CCANNNNNNTGG	1 cut(s)	2206
BstYI	RGATCY	2 cut(s)	1853, 2331
BstZ17I	GTATAC	2 cut(s)	846, 1766
Bsu15I	ATCGAT	1 cut(s)	2203
Bsu36I	CCTNAGG	2 cut(s)	164, 2706
BsuRI	GGCC	5 cut(s)	609, 1311, 1400, 1584, 2158
BsuTUI	ATCGAT	1 cut(s)	2203
BtgZI	GCGATG	2 cut(s)	165, 1463
BtrI	CACGTC	1 cut(s)	2195
BtsIMutI	CAGTG	3 cut(s)	1265, 1592, 2671
BveI	ACCTGC	4 cut(s)	133, 811, 1243, 2745
Cac8I	GCNNGC	9 cut(s)	969, 1369, 1477, 1689, 1864, 1888, 2080, 2173, 2284
CaiI	CAGNNNCTG	1 cut(s)	1175
CciI	TCATGA	1 cut(s)	2127
Cfr13I	GGNCC	2 cut(s)	1309, 1398
ClaI	ATCGAT	1 cut(s)	2203
CseI	GACGC	1 cut(s)	453
CsiI	ACCWGGT	1 cut(s)	1143
Csp6I	GTAC	5 cut(s)	348, 367, 499, 554, 1457
CviQI	GTAC	5 cut(s)	348, 367, 499, 554, 1457
DpnI	GATC	8 cut(s)	687, 1548, 1855, 1967, 2049, 2184, 2333, 2429
DpnII	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
EaeI	YGGCCR	2 cut(s)	1582, 2156
Eam1104I	CTCTTC	4 cut(s)	2073, 2205, 2277, 2631
EarI	CTCTTC	4 cut(s)	2073, 2205, 2277, 2631
Ecl136II	GAGCTC	2 cut(s)	256, 1866
Eco24I	GRGCYC	2 cut(s)	258, 1868
Eco32I	GATATC	1 cut(s)	1665
Eco53kI	GAGCTC	2 cut(s)	256, 1866
Eco57I	CTGAAG	3 cut(s)	1922, 1999, 2277
Eco81I	CCTNAGG	2 cut(s)	164, 2706
Eco88I	CYCGRG	2 cut(s)	215, 518
EcoICRI	GAGCTC	2 cut(s)	256, 1866
EcoO109I	RGGNCCY	1 cut(s)	1398
EcoRI	GAATTC	2 cut(s)	748, 958
EcoRII	CCWGG	5 cut(s)	609, 1143, 2153, 2356, 2461
EcoRV	GATATC	1 cut(s)	1665
EcoT22I	ATGCAT	2 cut(s)	1912, 1986
EcoT38I	GRGCYC	2 cut(s)	258, 1868
Esp3I	CGTCTC	1 cut(s)	18
FalI	AAGNNNNNCTT	2 cut(s)	2318, 2350
FaqI	GGGAC	1 cut(s)	840
FauI	CCCGC	4 cut(s)	504, 1895, 2733, 2787
FauNDI	CATATG	1 cut(s)	2686
FbaI	TGATCA	2 cut(s)	1965, 2047
FblI	GTMKAC	3 cut(s)	845, 1765, 2692
FriOI	GRGCYC	2 cut(s)	258, 1868
FspBI	CTAG	6 cut(s)	659, 1782, 1830, 2394, 2511, 2774
GsaI	CCCAGC	2 cut(s)	76, 86
HaeIII	GGCC	5 cut(s)	609, 1311, 1400, 1584, 2158
HapII	CCGG	1 cut(s)	318
HgaI	GACGC	1 cut(s)	453
HincII	GTYRAC	2 cut(s)	652, 2246
HindII	GTYRAC	2 cut(s)	652, 2246
HindIII	AAGCTT	2 cut(s)	171, 353
HpaI	GTTAAC	1 cut(s)	652
HpaII	CCGG	1 cut(s)	318
Hpy166II	GTNNAC	7 cut(s)	540, 652, 846, 1300, 1766, 2246, 2693
Hpy188III	TCNNGA	9 cut(s)	206, 491, 530, 809, 1200, 1703, 2045, 2128, 2705
Hpy8I	GTNNAC	7 cut(s)	540, 652, 846, 1300, 1766, 2246, 2693
HpyCH4III	ACNGT	6 cut(s)	23, 503, 1410, 1820, 1928, 2666
HpyCH4IV	ACGT	3 cut(s)	842, 2057, 2194
HpyF10VI	GCNNNNNNNGC	8 cut(s)	156, 253, 569, 671, 770, 866, 1373, 1697
HpySE526I	ACGT	3 cut(s)	842, 2057, 2194
Ksp22I	TGATCA	2 cut(s)	1965, 2047
KspAI	GTTAAC	1 cut(s)	652
Kzo9I	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
LguI	GCTCTTC	2 cut(s)	2073, 2277
LmnI	GCTCC	4 cut(s)	233, 252, 386, 475
LweI	GCATC	8 cut(s)	1181, 1317, 1435, 1735, 1971, 2013, 2640, 2803
MabI	ACCWGGT	1 cut(s)	1143
MaeI	CTAG	6 cut(s)	659, 1782, 1830, 2394, 2511, 2774
MaeII	ACGT	3 cut(s)	842, 2057, 2194
MaeIII	GTNAC	6 cut(s)	23, 503, 1388, 1520, 1592, 1712
MalI	GATC	8 cut(s)	687, 1548, 1855, 1967, 2049, 2184, 2333, 2429
MbiI	CCGCTC	1 cut(s)	247
MboI	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
MfeI	CAATTG	1 cut(s)	1384
MflI	RGATCY	2 cut(s)	1853, 2331
MhlI	GDGCHC	4 cut(s)	258, 1868, 2272, 2348
MlsI	TGGCCA	2 cut(s)	1584, 2158
MluNI	TGGCCA	2 cut(s)	1584, 2158
MlyI	GAGTC	1 cut(s)	2251
MmeI	TCCRAC	2 cut(s)	76, 2453
Mox20I	TGGCCA	2 cut(s)	1584, 2158
Mph1103I	ATGCAT	2 cut(s)	1912, 1986
MroXI	GAANNNNTTC	3 cut(s)	600, 1838, 2848
MscI	TGGCCA	2 cut(s)	1584, 2158
MseI	TTAA	6 cut(s)	312, 576, 651, 1017, 1403, 2697
MslI	CAYNNNNRTG	2 cut(s)	1218, 1989
Msp20I	TGGCCA	2 cut(s)	1584, 2158
MspA1I	CMGCKG	1 cut(s)	1890
MspCI	CTTAAG	1 cut(s)	2696
MspI	CCGG	1 cut(s)	318
MspR9I	CCNGG	5 cut(s)	611, 1145, 2155, 2358, 2463
MunI	CAATTG	1 cut(s)	1384
Mva1269I	GAATGC	2 cut(s)	397, 1912
MvaI	CCWGG	5 cut(s)	611, 1145, 2155, 2358, 2463
MvnI	CGCG	1 cut(s)	338
MwoI	GCNNNNNNNGC	8 cut(s)	156, 253, 569, 671, 770, 866, 1373, 1697
NdeI	CATATG	1 cut(s)	2686
NdeII	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
NlaIV	GGNNCC	4 cut(s)	382, 477, 525, 2347
NmeAIII	GCCGAG	1 cut(s)	2312
NmuCI	GTSAC	3 cut(s)	503, 1388, 1712
NsiI	ATGCAT	2 cut(s)	1912, 1986
NspI	RCATGY	3 cut(s)	511, 567, 2414
NspV	TTCGAA	1 cut(s)	2722
PaeR7I	CTCGAG	1 cut(s)	215
PagI	TCATGA	1 cut(s)	2127
PaqCI	CACCTGC	1 cut(s)	811
PciI	ACATGT	1 cut(s)	2410
PciSI	GCTCTTC	2 cut(s)	2073, 2277
PcsI	WCGNNNNNNNCGW	1 cut(s)	2200
PctI	GAATGC	2 cut(s)	397, 1912
PdmI	GAANNNNTTC	3 cut(s)	600, 1838, 2848
PfoI	TCCNGGA	2 cut(s)	2356, 2461
PleI	GAGTC	1 cut(s)	2250
PpsI	GAGTC	1 cut(s)	2250
Ppu21I	YACGTR	1 cut(s)	843
PscI	ACATGT	1 cut(s)	2410
Psp124BI	GAGCTC	2 cut(s)	258, 1868
Psp6I	CCWGG	5 cut(s)	609, 1143, 2153, 2356, 2461
PspFI	CCCAGC	2 cut(s)	72, 82
PspGI	CCWGG	5 cut(s)	609, 1143, 2153, 2356, 2461
PspN4I	GGNNCC	4 cut(s)	382, 477, 525, 2347
PspPI	GGNCC	2 cut(s)	1309, 1398
PspXI	VCTCGAGB	1 cut(s)	215
PsrI	GAACNNNNNNTAC	2 cut(s)	1497, 1529
PstI	CTGCAG	1 cut(s)	2084
PstNI	CAGNNNCTG	1 cut(s)	1175
PsuI	RGATCY	2 cut(s)	1853, 2331
RsaI	GTAC	5 cut(s)	349, 368, 500, 555, 1458
RsaNI	GTAC	5 cut(s)	348, 367, 499, 554, 1457
RseI	CAYNNNNRTG	2 cut(s)	1218, 1989
SacI	GAGCTC	2 cut(s)	258, 1868
SapI	GCTCTTC	2 cut(s)	2073, 2277
SaqAI	TTAA	6 cut(s)	312, 576, 651, 1017, 1403, 2697
Sau3AI	GATC	8 cut(s)	685, 1546, 1853, 1965, 2047, 2182, 2331, 2427
Sau96I	GGNCC	2 cut(s)	1309, 1398
SchI	GAGTC	1 cut(s)	2251
ScrFI	CCNGG	5 cut(s)	611, 1145, 2155, 2358, 2463
SduI	GDGCHC	4 cut(s)	258, 1868, 2272, 2348
SexAI	ACCWGGT	1 cut(s)	1143
SfaNI	GCATC	8 cut(s)	1181, 1317, 1435, 1735, 1971, 2013, 2640, 2803
SfcI	CTRYAG	1 cut(s)	2080
Sfr274I	CTCGAG	1 cut(s)	215
SfuI	TTCGAA	1 cut(s)	2722
SlaI	CTCGAG	1 cut(s)	215
SmiMI	CAYNNNNRTG	2 cut(s)	1218, 1989
SmlI	CTYRAG	4 cut(s)	215, 251, 491, 2696
SmoI	CTYRAG	4 cut(s)	215, 251, 491, 2696
SpeI	ACTAGT	1 cut(s)	2773
SsiI	CCGC	7 cut(s)	245, 336, 511, 1779, 1888, 2740, 2794
SspMI	CTAG	6 cut(s)	659, 1782, 1830, 2394, 2511, 2774
SstI	GAGCTC	2 cut(s)	258, 1868
StyD4I	CCNGG	5 cut(s)	609, 1143, 2153, 2356, 2461
TaaI	ACNGT	6 cut(s)	23, 503, 1410, 1820, 1928, 2666
TaiI	ACGT	3 cut(s)	845, 2060, 2197
TaqI	TCGA	7 cut(s)	216, 808, 1239, 2203, 2378, 2722, 2835
TatI	WGTACW	1 cut(s)	498
TauI	GCSGC	2 cut(s)	247, 1781
Tru1I	TTAA	6 cut(s)	312, 576, 651, 1017, 1403, 2697
Tru9I	TTAA	6 cut(s)	312, 576, 651, 1017, 1403, 2697
TscAI	CASTG	3 cut(s)	1272, 1592, 2671
TseFI	GTSAC	3 cut(s)	503, 1388, 1712
Tsp45I	GTSAC	3 cut(s)	503, 1388, 1712
TspGWI	ACGGA	2 cut(s)	373, 775
TspRI	CASTG	3 cut(s)	1272, 1592, 2671
Vha464I	CTTAAG	1 cut(s)	2696
XapI	RAATTY	6 cut(s)	101, 600, 748, 958, 1812, 2541
XceI	RCATGY	3 cut(s)	511, 567, 2414
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2469
XhoI	CTCGAG	1 cut(s)	215
XmiI	GTMKAC	3 cut(s)	845, 1765, 2692
XmnI	GAANNNNTTC	3 cut(s)	600, 1838, 2848
XspI	CTAG	6 cut(s)	659, 1782, 1830, 2394, 2511, 2774
Zsp2I	ATGCAT	2 cut(s)	1912, 1986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.