Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009781

Physical Location & Seq

Genomic Coordinates Forward (+)

512324 .. 519795

Gene Structure 7472 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1003400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2124 bp

ATGCCAGAGTTGCGAAGCGGAGTGCGCAGATCAAAACGGCTTGATGATCTTCAGCCTTCCTCACAACCACCAATTGGTCAGGCCGAAAATTGTGTACTACCTGCCCAGAATAAACCTCGGAGAAGAGCAGGTGGTGGTAGGGGAAGAGGTGGTAATGCTGTAGCAAAAGGTCCTTCACCGGCAATACCCACTAGGCAGACTGCAGCTGGTAGAGGTCGGGGCGTTAGGTTGATAGATTTAGACCCTGAACCTTGCGAGGTCCGTCCTGAAGCTGCTGCTTTAGGAGCTGCTGAACCCATTTACAATCGTGCGGACGTTGTAGCAGATAAAGATATTGCGATGGAGGGTGGTGGAAGTGCGGACAAAATAGTGGGAGGTGAAGAAGAAGCAGGCGGGACTCCTGTTCCTGATAGGGTACAAGTTGGTAACTCTCCTGTGTACAAGGTAGAAAGGAAGTTGGGTAAGGGTGGTTTTGGGCAAGTTTATGTTGGCAGAAGGGTTAGTGGTGGCACTGATAGGACAGGACCTGACGCTATTGAGGTTGCTTTGAAGTTTGAACACCGAAATAGTAAAGGTTGCAATTACGGCCCTCCTTATGAGTGGCAAGTTTACAATAATCTAAATGGATGTTATGGAATTCCTTGGGTTCATTACAAGGGTCGCCAGGGAGACTTTTACATTCTGGTCATGGACATTCTTGGACCCAGTCTTTGGGATGTTTGGAATTCTCTTGGGCAATCGATGTCTCCAAATATGGTGGCATGCATTGCCGTAGAGGCAATATCAATTCTTGAAAAGCTTCACTTAAAAGGGTTTGTGCATGGCGATGTAAAACCAGAGAATTTTTTACTTGGTCAACCTGCTACACCTGATGAGAAGAAGCTATATCTTATTGATCTTGGATTGGCGTCTAAATGGAAGGAAGCATCTGGTCAACATGTTGAATATGATCAGAGGCCTGACATATTCAGAGGAACAATAAGGTATGCAAGTGTACATGCACATTTAGGTCGGACTGGAAGTCGAAGGGATGATCTTGAGTCATTGGCATACACATTAATATTTCTCATAAAAGGAAGGTTACCCTGGCAGGGTTATCAGGGGGATAACAAGAGTTTTCTTGTTTGTAAGAAAAAGATGGCCACCTCTCCAGAGTTGATGTGCTGCTTCTGCCCTCCCCCATTCAAACAGTTTCTTGAAGCTATTACAATTATGAAGTTTGATGAAGAACCAAATTATGCAAAGTTGATATCCTTCTTTGAAAGCTTGATCGAGCCATGCCTGCCACTAAGACCAATTAGAATTGATGGAGCTCTTAAGGTAGGACAAAAACGGGGAAGAATGTCTATAAATTTGGAAGAAGATGAACAGCCAAAGAAGAAAGTTCGACTGGGTAGTCCTGCTACCCAGTGGATTTCAGTGTACAATGCGCGTCGTCCAATGAAGCAAAGGTATCACTACAATGTGGCGGACTCAAGGCTGCATCAGCATGTCGATAAGGGTAATGAAGATGGCTTGTATATCAGCTGTGTAGCGTCTTCATCAAGTCTATGGGCCTTAATCATGGATGCAGGGACAAGTTTCACTGCCCAGGTTTATGAGCTGTCAACTGTCTTCCTGCATAAGGATTGGATCATGGAACAGTGGGAAAACAACTTCTATATTAGTTCAATAGCTGGTGCGGCTAATGGGAGTTCCTTGGTTGTTATGTCCAAAGGTACTCCATACACCCAGCAGTCGTACAAGGTAAGCGAATCTTTTCCCTTCAAGTGGATAAATAAGAAGTGGAAAGAAGGTTTCCATGTAACATCCATGACAACCGCTGGCAATCGCTGGGGTGTTGTTATGTCCAGGAATGCTGGATTTTCGGACCAGGTTGTGGAGCTTGATTTTGTATACCCAAGTGAAGGCATCCACAGGCGATGGGAAAGTGGCTACAGAATAACATCCATGGCTGCCACCGCTGATCAAGCAGCCTTCATTTTGAGCATACCAAAACGCAAGTTGATGGATGAAACCCAAGAAACTCTGCGTACCTCCGCCTTCCCAAGCAGCCATGTAAAGGAAAAATGGTCAAAAAATCTCTATATTGCATCGATTTGTTACGGACGGACCGTTTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007623 GO:0008150 GO:0008152 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010468 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0030162 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032501 GO:0032922 GO:0036211 GO:0042176 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045732 GO:0045862 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0061136 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:2000058 GO:2000060

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

708

Amino Acids

78.72

Weight (kDa)

9.04

Isoelectric Point (pI)

46.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	147 - 378	8e-16	Protein kinase domain
Pkinase_fungal	PF17667	258 - 356	1e-08	Fungal protein kinase
DUF7477	PF24289	440 - 706	2.9e-160	Domain of unknown function (DUF7477)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009781)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03940 AT5G18190 AT5G18190
fragaria_vesca	FvH4_1g28110 FvH4_1g28110 FvH4_1g28110 FvH4_1g28110
malus_domestica	MD12G1003400.v1.1
prunus_persica	Prupe.7G003800_v2.0.a1
pyrus_communis	pycom12g00290
rosa_chinensis	RchiOBHm_Chr3g0497131
rosa_laevigata	RLG00000022480
rosa_multiflora	Rmu_ssc0000003.1_g000001
rosa_roxburghii	Rroxscaffold_6G00388390
rosa_rugosa	Rorug03G0290200 Rorug03G0290300
rosa_samantha	Rh3AG334300 Rh3BG371400 Rh3CG368300 Rh3DG369300
rosa_wichuraiana	Rw3G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	119
AasI	GACNNNNNNGTC	1 cut(s)	1395
Acc16I	TGCGCA	1 cut(s)	26
Acc36I	ACCTGC	3 cut(s)	109, 119, 868
AccB7I	CCANNNNNTGG	3 cut(s)	74, 711, 1879
AccI	GTMKAC	1 cut(s)	1896
AccII	CGCG	1 cut(s)	1432
AciI	CCGC	9 cut(s)	18, 311, 359, 393, 1469, 1682, 1821, 1962, 2040
AclWI	GGATC	1 cut(s)	1640
AcoI	YGGCCR	1 cut(s)	1140
AcsI	RAATTY	4 cut(s)	636, 724, 841, 1351
AcuI	CTGAAG	2 cut(s)	35, 288
AcyI	GRCGYC	1 cut(s)	908
AfaI	GTAC	8 cut(s)	96, 417, 440, 996, 1424, 1720, 1742, 2035
AfiI	CCNNNNNNNGG	8 cut(s)	74, 711, 1091, 1624, 1770, 1879, 1907, 2062
AflII	CTTAAG	1 cut(s)	1316
AflIII	ACRYGT	1 cut(s)	937
AgsI	TTSAA	9 cut(s)	550, 557, 794, 944, 1186, 1199, 1262, 1671, 1768
AhdI	GACNNNNNGTC	1 cut(s)	1020
AjnI	CCWGG	5 cut(s)	663, 1085, 1590, 1850, 1872
Alw21I	GWGCWC	1 cut(s)	1315
Alw26I	GTCTC	2 cut(s)	663, 750
AlwI	GGATC	1 cut(s)	1640
AlwNI	CAGNNNCTG	1 cut(s)	527
AoxI	GGCC	5 cut(s)	81, 586, 956, 1140, 1554
ApeKI	GCWGC	9 cut(s)	203, 272, 275, 287, 1164, 1480, 1955, 1973, 2052
ApoI	RAATTY	4 cut(s)	636, 724, 841, 1351
ArsI	GACNNNNNNTTYG	3 cut(s)	693, 725, 2100
AseI	ATTAAT	1 cut(s)	1058
Asp700I	GAANNNNTTC	2 cut(s)	798, 1758
AspLEI	GCGC	2 cut(s)	27, 1432
AspS9I	GGNCC	8 cut(s)	170, 259, 524, 587, 701, 1554, 1870, 2112
AsuHPI	GGTGA	2 cut(s)	168, 389
AvaII	GGWCC	6 cut(s)	170, 259, 524, 701, 1870, 2112
BalI	TGGCCA	1 cut(s)	1142
BanII	GRGCYC	1 cut(s)	1315
BbsI	GAAGAC	2 cut(s)	1530, 1606
Bbv12I	GWGCWC	1 cut(s)	1315
BbvI	GCAGC	9 cut(s)	215, 259, 262, 274, 1151, 1467, 1942, 1985, 2064
BccI	CCATC	6 cut(s)	334, 1132, 1301, 1505, 1917, 2002
BceAI	ACGGC	3 cut(s)	53, 601, 755
BciT130I	CCWGG	5 cut(s)	665, 1087, 1592, 1852, 1874
BclI	TGATCA	2 cut(s)	949, 1966
BcoDI	GTCTC	2 cut(s)	663, 750
BfaI	CTAG	1 cut(s)	192
BfmI	CTRYAG	3 cut(s)	159, 201, 1936
BfrI	CTTAAG	1 cut(s)	1316
BfuAI	ACCTGC	3 cut(s)	109, 119, 868
BglI	GCCNNNNNGGC	1 cut(s)	776
Bme1390I	CCNGG	5 cut(s)	665, 1087, 1592, 1852, 1874
Bme18I	GGWCC	6 cut(s)	170, 259, 524, 701, 1870, 2112
BmeRI	GACNNNNNGTC	1 cut(s)	1020
BmgT120I	GGNCC	8 cut(s)	170, 259, 524, 587, 701, 1554, 1870, 2112
BmiI	GGNNCC	1 cut(s)	703
BmrFI	CCNGG	5 cut(s)	665, 1087, 1592, 1852, 1874
BmrI	ACTGGG	3 cut(s)	699, 1400, 1402
BmsI	GCATC	5 cut(s)	935, 1492, 1558, 1920, 2102
BmuI	ACTGGG	3 cut(s)	699, 1400, 1402
BpiI	GAAGAC	2 cut(s)	1530, 1606
BpmI	CTGGAG	1 cut(s)	1134
BpuEI	CTTGAG	2 cut(s)	1058, 1459
Bsa29I	ATCGAT	2 cut(s)	740, 2096
BsaHI	GRCGYC	1 cut(s)	908
BsaJI	CCNNGG	7 cut(s)	116, 641, 664, 1085, 1590, 1698, 1950
Bsc4I	CCNNNNNNNGG	8 cut(s)	74, 711, 1091, 1624, 1770, 1879, 1907, 2062
Bse118I	RCCGGY	1 cut(s)	178
Bse1I	ACTGG	4 cut(s)	705, 1021, 1395, 1408
Bse3DI	GCAATG	1 cut(s)	765
BseBI	CCWGG	5 cut(s)	665, 1087, 1592, 1852, 1874
BseCI	ATCGAT	2 cut(s)	740, 2096
BseDI	CCNNGG	7 cut(s)	116, 641, 664, 1085, 1590, 1698, 1950
BseGI	GGATG	8 cut(s)	632, 721, 1036, 1573, 1808, 1911, 1946, 2017
BseLI	CCNNNNNNNGG	8 cut(s)	74, 711, 1091, 1624, 1770, 1879, 1907, 2062
BseMI	GCAATG	1 cut(s)	765
BseNI	ACTGG	4 cut(s)	705, 1021, 1395, 1408
BseXI	GCAGC	9 cut(s)	215, 259, 262, 274, 1151, 1467, 1942, 1985, 2064
BseYI	CCCAGC	2 cut(s)	1731, 1833
Bsh1236I	CGCG	1 cut(s)	1432
BshFI	GGCC	5 cut(s)	83, 588, 958, 1142, 1556
BshVI	ATCGAT	2 cut(s)	740, 2096
BsiHKAI	GWGCWC	1 cut(s)	1315
BsiSI	CCGG	1 cut(s)	179
BslFI	GGGAC	2 cut(s)	409, 1588
BslI	CCNNNNNNNGG	8 cut(s)	74, 711, 1091, 1624, 1770, 1879, 1907, 2062
BsmAI	GTCTC	2 cut(s)	663, 750
BsmFI	GGGAC	2 cut(s)	409, 1588
BsmI	GAATGC	1 cut(s)	1861
BsnI	GGCC	5 cut(s)	83, 588, 958, 1142, 1556
Bsp1286I	GDGCHC	1 cut(s)	1315
Bsp1407I	TGTACA	3 cut(s)	438, 994, 1422
Bsp143I	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
Bsp19I	CCATGG	1 cut(s)	1950
BspACI	CCGC	9 cut(s)	18, 311, 359, 393, 1469, 1682, 1821, 1962, 2040
BspANI	GGCC	5 cut(s)	83, 588, 958, 1142, 1556
BspDI	ATCGAT	2 cut(s)	740, 2096
BspFNI	CGCG	1 cut(s)	1432
BspLI	GGNNCC	1 cut(s)	703
BspMAI	CTGCAG	1 cut(s)	205
BspMI	ACCTGC	3 cut(s)	109, 119, 868
BspPI	GGATC	1 cut(s)	1640
BspQI	GCTCTTC	1 cut(s)	118
BspTI	CTTAAG	1 cut(s)	1316
BsrDI	GCAATG	1 cut(s)	765
BsrFI	RCCGGY	1 cut(s)	178
BsrGI	TGTACA	3 cut(s)	438, 994, 1422
BsrI	ACTGG	4 cut(s)	705, 1021, 1395, 1408
BssAI	RCCGGY	1 cut(s)	178
BssECI	CCNNGG	7 cut(s)	116, 641, 664, 1085, 1590, 1698, 1950
BssMI	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
BssNAI	GTATAC	1 cut(s)	1897
BssNI	GRCGYC	1 cut(s)	908
BssT1I	CCWWGG	3 cut(s)	641, 1698, 1950
Bst1107I	GTATAC	1 cut(s)	1897
Bst2UI	CCWGG	5 cut(s)	665, 1087, 1592, 1852, 1874
Bst4CI	ACNGT	4 cut(s)	1191, 1612, 1644, 2116
Bst6I	CTCTTC	2 cut(s)	118, 139
BstACI	GRCGYC	1 cut(s)	908
BstAFI	CTTAAG	1 cut(s)	1316
BstAPI	GCANNNNNTGC	1 cut(s)	767
BstAUI	TGTACA	3 cut(s)	438, 994, 1422
BstC8I	GCNNGC	4 cut(s)	391, 763, 1283, 1825
BstDEI	CTNAG	1 cut(s)	1289
BstDSI	CCRYGG	1 cut(s)	1950
BstEII	GGTNACC	1 cut(s)	1080
BstENI	CCTNNNNNAGG	1 cut(s)	1622
BstF5I	GGATG	8 cut(s)	632, 721, 1036, 1573, 1808, 1911, 1946, 2017
BstFNI	CGCG	1 cut(s)	1432
BstHHI	GCGC	2 cut(s)	27, 1432
BstKTI	GATC	8 cut(s)	32, 49, 898, 952, 1036, 1272, 1635, 1969
BstMAI	GTCTC	2 cut(s)	663, 750
BstMBI	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
BstNI	CCWGG	5 cut(s)	665, 1087, 1592, 1852, 1874
BstNSI	RCATGY	4 cut(s)	765, 941, 1001, 1493
BstPI	GGTNACC	1 cut(s)	1080
BstSCI	CCNGG	5 cut(s)	663, 1085, 1590, 1850, 1872
BstSFI	CTRYAG	3 cut(s)	159, 201, 1936
BstUI	CGCG	1 cut(s)	1432
BstV1I	GCAGC	9 cut(s)	215, 259, 262, 274, 1151, 1467, 1942, 1985, 2064
BstV2I	GAAGAC	2 cut(s)	1530, 1606
BstZ17I	GTATAC	1 cut(s)	1897
Bsu15I	ATCGAT	2 cut(s)	740, 2096
BsuRI	GGCC	5 cut(s)	83, 588, 958, 1142, 1556
BsuTUI	ATCGAT	2 cut(s)	740, 2096
BtgI	CCRYGG	1 cut(s)	1950
BtgZI	GCGATG	3 cut(s)	353, 840, 1936
BtsCI	GGATG	8 cut(s)	632, 721, 1036, 1573, 1808, 1911, 1946, 2017
BtsI	GCAGTG	1 cut(s)	1584
BtsIMutI	CAGTG	5 cut(s)	510, 1415, 1425, 1584, 1649
BveI	ACCTGC	3 cut(s)	109, 119, 868
Cac8I	GCNNGC	4 cut(s)	391, 763, 1283, 1825
CaiI	CAGNNNCTG	1 cut(s)	527
CfoI	GCGC	2 cut(s)	27, 1432
Cfr10I	RCCGGY	1 cut(s)	178
Cfr13I	GGNCC	8 cut(s)	170, 259, 524, 587, 701, 1554, 1870, 2112
ClaI	ATCGAT	2 cut(s)	740, 2096
CpoI	CGGWCCG	1 cut(s)	2112
CseI	GACGC	4 cut(s)	539, 897, 1421, 1524
CsiI	ACCWGGT	1 cut(s)	1872
Csp6I	GTAC	8 cut(s)	95, 416, 439, 995, 1423, 1719, 1741, 2034
CspCI	CAANNNNNGTGG	2 cut(s)	738, 773
CspI	CGGWCCG	1 cut(s)	2112
CviQI	GTAC	8 cut(s)	95, 416, 439, 995, 1423, 1719, 1741, 2034
DdeI	CTNAG	1 cut(s)	1289
DpnI	GATC	8 cut(s)	31, 48, 897, 951, 1035, 1271, 1634, 1968
DpnII	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
DrdI	GACNNNNNNGTC	1 cut(s)	1395
DriI	GACNNNNNGTC	1 cut(s)	1020
DseDI	GACNNNNNNGTC	1 cut(s)	1395
EaeI	YGGCCR	1 cut(s)	1140
Eam1104I	CTCTTC	2 cut(s)	118, 139
Eam1105I	GACNNNNNGTC	1 cut(s)	1020
EarI	CTCTTC	2 cut(s)	118, 139
EciI	GGCGGA	2 cut(s)	1484, 2029
Ecl136II	GAGCTC	1 cut(s)	1313
Eco130I	CCWWGG	3 cut(s)	641, 1698, 1950
Eco147I	AGGCCT	1 cut(s)	958
Eco24I	GRGCYC	1 cut(s)	1315
Eco32I	GATATC	1 cut(s)	1251
Eco47I	GGWCC	6 cut(s)	170, 259, 524, 701, 1870, 2112
Eco53kI	GAGCTC	1 cut(s)	1313
Eco57I	CTGAAG	2 cut(s)	35, 288
Eco91I	GGTNACC	1 cut(s)	1080
EcoICRI	GAGCTC	1 cut(s)	1313
EcoNI	CCTNNNNNAGG	1 cut(s)	1622
EcoO109I	RGGNCCY	2 cut(s)	170, 524
EcoO65I	GGTNACC	1 cut(s)	1080
EcoRI	GAATTC	2 cut(s)	636, 724
EcoRII	CCWGG	5 cut(s)	663, 1085, 1590, 1850, 1872
EcoRV	GATATC	1 cut(s)	1251
EcoT14I	CCWWGG	3 cut(s)	641, 1698, 1950
EcoT22I	ATGCAT	1 cut(s)	767
EcoT38I	GRGCYC	1 cut(s)	1315
ErhI	CCWWGG	3 cut(s)	641, 1698, 1950
FalI	AAGNNNNNCTT	4 cut(s)	788, 820, 1741, 1773
FaqI	GGGAC	2 cut(s)	409, 1588
FauI	CCCGC	1 cut(s)	386
FbaI	TGATCA	2 cut(s)	949, 1966
FblI	GTMKAC	1 cut(s)	1896
FokI	GGATG	8 cut(s)	639, 728, 1043, 1580, 1795, 1898, 1933, 2024
FriOI	GRGCYC	1 cut(s)	1315
FspBI	CTAG	1 cut(s)	192
FspI	TGCGCA	1 cut(s)	26
GlaI	GCGC	2 cut(s)	26, 1431
GsaI	CCCAGC	2 cut(s)	1735, 1837
GsuI	CTGGAG	1 cut(s)	1134
HaeIII	GGCC	5 cut(s)	83, 588, 958, 1142, 1556
HapII	CCGG	1 cut(s)	179
HgaI	GACGC	4 cut(s)	539, 897, 1421, 1524
HhaI	GCGC	2 cut(s)	27, 1432
Hin1I	GRCGYC	1 cut(s)	908
Hin6I	GCGC	2 cut(s)	25, 1430
HinP1I	GCGC	2 cut(s)	25, 1430
HincII	GTYRAC	3 cut(s)	857, 935, 1608
HindII	GTYRAC	3 cut(s)	857, 935, 1608
HindIII	AAGCTT	2 cut(s)	797, 1264
HinfI	GANTC	4 cut(s)	397, 1040, 1472, 1754
HpaII	CCGG	1 cut(s)	179
HphI	GGTGA	2 cut(s)	168, 389
Hpy166II	GTNNAC	9 cut(s)	95, 439, 610, 857, 935, 995, 1423, 1608, 1897
Hpy188I	TCNGA	5 cut(s)	120, 954, 971, 1014, 1870
Hpy188III	TCNNGA	6 cut(s)	266, 407, 791, 1037, 1151, 1196
Hpy8I	GTNNAC	9 cut(s)	95, 439, 610, 857, 935, 995, 1423, 1608, 1897
Hpy99I	CGWCG	1 cut(s)	1437
HpyCH4III	ACNGT	4 cut(s)	1191, 1612, 1644, 2116
HpyCH4IV	ACGT	1 cut(s)	315
HpyF3I	CTNAG	1 cut(s)	1289
HpySE526I	ACGT	1 cut(s)	315
Hsp92I	GRCGYC	1 cut(s)	908
HspAI	GCGC	2 cut(s)	25, 1430
Ksp22I	TGATCA	2 cut(s)	949, 1966
Kzo9I	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
LguI	GCTCTTC	1 cut(s)	118
LmnI	GCTCC	3 cut(s)	284, 1310, 1882
Lsp1109I	GCAGC	9 cut(s)	215, 259, 262, 274, 1151, 1467, 1942, 1985, 2064
LweI	GCATC	5 cut(s)	935, 1492, 1558, 1920, 2102
MabI	ACCWGGT	1 cut(s)	1872
MaeI	CTAG	1 cut(s)	192
MaeII	ACGT	1 cut(s)	315
MaeIII	GTNAC	4 cut(s)	425, 1080, 1804, 2102
MalI	GATC	8 cut(s)	31, 48, 897, 951, 1035, 1271, 1634, 1968
MboI	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
MfeI	CAATTG	1 cut(s)	72
MhlI	GDGCHC	1 cut(s)	1315
MlsI	TGGCCA	1 cut(s)	1142
MluNI	TGGCCA	1 cut(s)	1142
MlyI	GAGTC	3 cut(s)	391, 1049, 1466
MmeI	TCCRAC	1 cut(s)	992
Mox20I	TGGCCA	1 cut(s)	1142
Mph1103I	ATGCAT	1 cut(s)	767
MroXI	GAANNNNTTC	2 cut(s)	798, 1758
MscI	TGGCCA	1 cut(s)	1142
MseI	TTAA	4 cut(s)	806, 1058, 1317, 1559
MslI	CAYNNNNRTG	3 cut(s)	825, 1461, 1488
Msp20I	TGGCCA	1 cut(s)	1142
MspA1I	CMGCKG	4 cut(s)	206, 1527, 1823, 1964
MspCI	CTTAAG	1 cut(s)	1316
MspI	CCGG	1 cut(s)	179
MspR9I	CCNGG	5 cut(s)	665, 1087, 1592, 1852, 1874
MunI	CAATTG	1 cut(s)	72
Mva1269I	GAATGC	1 cut(s)	1861
MvaI	CCWGG	5 cut(s)	665, 1087, 1592, 1852, 1874
MvnI	CGCG	1 cut(s)	1432
NcoI	CCATGG	1 cut(s)	1950
NdeII	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
NlaIV	GGNNCC	1 cut(s)	703
NsbI	TGCGCA	1 cut(s)	26
NsiI	ATGCAT	1 cut(s)	767
NspI	RCATGY	4 cut(s)	765, 941, 1001, 1493
PaeI	GCATGC	1 cut(s)	765
PaqCI	CACCTGC	1 cut(s)	119
PceI	AGGCCT	1 cut(s)	958
PciI	ACATGT	1 cut(s)	937
PciSI	GCTCTTC	1 cut(s)	118
PcsI	WCGNNNNNNNCGW	1 cut(s)	2112
PctI	GAATGC	1 cut(s)	1861
PdmI	GAANNNNTTC	2 cut(s)	798, 1758
PfeI	GAWTC	1 cut(s)	1754
PflFI	GACNNNGTC	1 cut(s)	705
PflMI	CCANNNNNTGG	3 cut(s)	74, 711, 1879
PfoI	TCCNGGA	1 cut(s)	1850
PleI	GAGTC	3 cut(s)	391, 1048, 1466
PpsI	GAGTC	3 cut(s)	391, 1048, 1466
PpuMI	RGGWCCY	2 cut(s)	170, 524
PscI	ACATGT	1 cut(s)	937
PshBI	ATTAAT	1 cut(s)	1058
Psp124BI	GAGCTC	1 cut(s)	1315
Psp5II	RGGWCCY	2 cut(s)	170, 524
Psp6I	CCWGG	5 cut(s)	663, 1085, 1590, 1850, 1872
PspEI	GGTNACC	1 cut(s)	1080
PspFI	CCCAGC	2 cut(s)	1731, 1833
PspGI	CCWGG	5 cut(s)	663, 1085, 1590, 1850, 1872
PspN4I	GGNNCC	1 cut(s)	703
PspPI	GGNCC	8 cut(s)	170, 259, 524, 587, 701, 1554, 1870, 2112
PspPPI	RGGWCCY	2 cut(s)	170, 524
PstI	CTGCAG	1 cut(s)	205
PstNI	CAGNNNCTG	1 cut(s)	527
PsyI	GACNNNGTC	1 cut(s)	705
PvuII	CAGCTG	2 cut(s)	206, 1527
RsaI	GTAC	8 cut(s)	96, 417, 440, 996, 1424, 1720, 1742, 2035
RsaNI	GTAC	8 cut(s)	95, 416, 439, 995, 1423, 1719, 1741, 2034
RseI	CAYNNNNRTG	3 cut(s)	825, 1461, 1488
Rsr2I	CGGWCCG	1 cut(s)	2112
RsrII	CGGWCCG	1 cut(s)	2112
SacI	GAGCTC	1 cut(s)	1315
SapI	GCTCTTC	1 cut(s)	118
SaqAI	TTAA	4 cut(s)	806, 1058, 1317, 1559
Sau3AI	GATC	8 cut(s)	29, 46, 895, 949, 1033, 1269, 1632, 1966
Sau96I	GGNCC	8 cut(s)	170, 259, 524, 587, 701, 1554, 1870, 2112
SchI	GAGTC	3 cut(s)	391, 1049, 1466
ScrFI	CCNGG	5 cut(s)	665, 1087, 1592, 1852, 1874
SduI	GDGCHC	1 cut(s)	1315
SexAI	ACCWGGT	1 cut(s)	1872
SfaNI	GCATC	5 cut(s)	935, 1492, 1558, 1920, 2102
SfcI	CTRYAG	3 cut(s)	159, 201, 1936
SinI	GGWCC	6 cut(s)	170, 259, 524, 701, 1870, 2112
SmiMI	CAYNNNNRTG	3 cut(s)	825, 1461, 1488
SmlI	CTYRAG	3 cut(s)	1037, 1316, 1474
SmoI	CTYRAG	3 cut(s)	1037, 1316, 1474
SphI	GCATGC	1 cut(s)	765
SseBI	AGGCCT	1 cut(s)	958
SsiI	CCGC	9 cut(s)	18, 311, 359, 393, 1469, 1682, 1821, 1962, 2040
SspI	AATATT	1 cut(s)	1062
SspMI	CTAG	1 cut(s)	192
SstI	GAGCTC	1 cut(s)	1315
StuI	AGGCCT	1 cut(s)	958
StyD4I	CCNGG	5 cut(s)	663, 1085, 1590, 1850, 1872
StyI	CCWWGG	3 cut(s)	641, 1698, 1950
TaaI	ACNGT	4 cut(s)	1191, 1612, 1644, 2116
TaiI	ACGT	1 cut(s)	318
TaqI	TCGA	6 cut(s)	740, 1024, 1272, 1387, 1494, 2096
TatI	WGTACW	4 cut(s)	94, 438, 994, 1422
TauI	GCSGC	1 cut(s)	1685
TfiI	GAWTC	1 cut(s)	1754
Tru1I	TTAA	4 cut(s)	806, 1058, 1317, 1559
Tru9I	TTAA	4 cut(s)	806, 1058, 1317, 1559
TscAI	CASTG	5 cut(s)	517, 1415, 1425, 1591, 1649
TseI	GCWGC	9 cut(s)	203, 272, 275, 287, 1164, 1480, 1955, 1973, 2052
TspDTI	ATGAA	9 cut(s)	638, 1229, 1239, 1380, 1457, 1521, 1530, 1969, 2028
TspGWI	ACGGA	2 cut(s)	251, 2121
TspRI	CASTG	5 cut(s)	517, 1415, 1425, 1591, 1649
Tth111I	GACNNNGTC	1 cut(s)	705
Van91I	CCANNNNNTGG	3 cut(s)	74, 711, 1879
Vha464I	CTTAAG	1 cut(s)	1316
VpaK11BI	GGWCC	6 cut(s)	170, 259, 524, 701, 1870, 2112
VspI	ATTAAT	1 cut(s)	1058
XagI	CCTNNNNNAGG	1 cut(s)	1622
XapI	RAATTY	4 cut(s)	636, 724, 841, 1351
XceI	RCATGY	4 cut(s)	765, 941, 1001, 1493
XmiI	GTMKAC	1 cut(s)	1896
XmnI	GAANNNNTTC	2 cut(s)	798, 1758
XspI	CTAG	1 cut(s)	192
Zsp2I	ATGCAT	1 cut(s)	767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.